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Information on EC 2.3.1.67 - 1-alkylglycerophosphocholine O-acetyltransferase and Organism(s) Rattus norvegicus

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Rattus norvegicus
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The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
lyso-paf-at, acetyl-coa:lyso-paf acetyltransferase, lyso-paf at, lyso-paf:acetyl-coa acetyltransferase, lyso-paf-acetyltransferase, acetyl-coa lyso-paf acetyltransferase, lyso-paf-act, lyso-pafat, lysopafat, lysopaf-at, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1-alkyl-2-lyso-sn-glycero-3-phosphocholine acetyltransferase
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-
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acetyl-CoA:1-alkyl-2-lyso-sn-glycero-3-phosphocholine 2-O-acetyltransferase
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-
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acetyl-CoA:lyso-PAF acetyltransferase
acetyltransferase, 1-alkyl-2-lysolecithin
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-
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acetyltransferase, 1-alkylglycerophosphocholine
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acyl-CoA:1-alkyl-sn-glycero-3-phosphocholine acyltransferase
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blood platelet-activating factor acetyltransferase
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-
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lyso-GPC:acetyl CoA acetyltransferase
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lyso-platelet activating factor:acetyl-CoA acetyltransferase
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lyso-platelet-activating factor:acetyl-CoA acetyltransferase
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lysoPAF:acetyl CoA acetyltransferase
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PAF acetyltransferase
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platelet-activating factor acylhydrolase
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platelet-activating factor-synthesizing enzyme
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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-
-
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PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:1-alkyl-sn-glycero-3-phosphocholine 2-O-acetyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
76773-96-1
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + 1-palmitoyl-2-lyso-sn-glycero-3-phosphocholine
CoA + 2-acetyl-1-palmitoyl-sn-glycero-3-phosphocholine
show the reaction diagram
acetyl-CoA + acyl-lyso-glycero-3-phosphocholine
CoA + acetyl-acyl-glycero-3-phosphocholine
show the reaction diagram
-
worse substrate than alkyl-lyso-glycero-3-phosphocholine
-
-
?
acetyl-CoA + alkyl-lyso-glycero-3-phosphoethanolamine
CoA + 2-acetyl-alkyl-glycero-3-phosphoethanolamine
show the reaction diagram
-
least enzyme activity
-
?
acetyl-CoA + alkyl-lyso-monomethylethanamine
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + alkyl-lyso-N',N'-dimethylethanamine
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + alkyl-sn-glycero-3-phosphocholine
CoA + 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine
show the reaction diagram
acetyl-CoA + octadecyl-lyso-sn-glycero-3-phosphocholine
CoA + 2-acetyl-1-octadecyl-sn-glycero-3-phosphocholine
show the reaction diagram
butyryl-CoA + 1-alkyl-2-lyso-sn-glycero-3-phosphocholine
2-butyryl-1-alkyl-sn-glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
hexanoyl-CoA + 1-alkyl-2-lyso-sn-glycero-3-phosphocholine
2-hexanoyl-1-alkyl-sn-glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + alkyl-lyso-glycero-3-phosphoethanolamine
CoA + 1-alkyl-2-oleoyl-glycero-3-phosphoethanolamine
show the reaction diagram
-
10% of the activity observed in presence of acetyl-CoA
-
?
palmitoyl-CoA + alkyl-lyso-glycero-3-phosphoethanolamine
CoA + acetyl-2-palmitoyl-glycero-3-phosphoethanolamine
show the reaction diagram
-
5% of the activity observed in presence of acetyl-CoA
-
?
propionyl-CoA + alkyl-lyso-glycero-phosphocholine
CoA + 2-propionyl-1-alkyl-sn-glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
activation reversed by addition of EGTA in excess of Ca2+, Ca2+ reduces the apparent Km for acetyl-CoA, maximum effect between 0.0001 and 0.01 mM, the action of Ca2+ seems to be independent of the presence of calmodulin or phosphorylation
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-methoxyphaseolidin
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IC50: 0.048 mM
1-methoxyphaseolin
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IC50: 0.057 mM
2-methoxy-5-methyl-3-tetradecanoyloxy-1,4-benzoquinone
-
derivative of a metabolite from Penicillium sp. F33 , strong inhibition. Compound also significantly suppresses the gene expression of lyso-PAF acetyltransferase/LPCAT2 in mouse bone marrow-derived macrophages stimulated by lipopolysaccharide
3-decanoyloxy-2-methoxy-5-methyl-1,4-benzoquinone
-
derivative of a metabolite from Penicillium sp. F33 , strong inhibition. Compound also significantly suppresses the gene expression of lyso-PAF acetyltransferase/LPCAT2 in mouse bone marrow-derived macrophages stimulated by lipopolysaccharide
4-(1-Naphthylvinyl)pyridine
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IC50: 0.043 mM
6,8-diprenylgenistein
-
IC50: 0.019 mM
acetyl-salicylic acid
-
weak inhibition
baicalein
-
IC50: 0.148 mM
CaCl2
-
15% inhibition
chiyusaponin
-
IC50: 0.2 mM
dexamethasone
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2 mg/kg for 3 days in liver and spleen
diisopropylfluorophosphate
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98% inhibition at 10 mM
honokiol
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IC50: 0.15 mM
indomethacin
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weak inhibition at 1 mM
licoricidin
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IC50: 0.0077 mM
luteolin
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IC50: 0.045 mM
magnolol
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IC50: 0.15 mM
medroxyprogesterone
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50 mg/kg in liver
MgCl2
-
98% inhibition
MnCl2
-
50% inhibition
nordihydroguaiaretic acid
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IC50: 0.29 mM
p-bromophenacyl bromide
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90% inhibition at 0.1 mM
palmitoyl-CoA
-
85% inhibition at 10 uM due to detergent effect
palmitoyllyso-glycero-3-phosphocholine
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competitive inhibitor for alkyl-sn-glycero-3-phosphocholine
quercetin
-
IC50: 0.08 mM
sodium dodecylsulfate
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inactivation at 0.1%
tetradecanoic acid
-
-
Trifluoperazine
-
-
Triton X-100
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75 and 95% inhibition at 0.2 and 0.6 mM respectively due to detergent
Urea
-
inactivation at 8 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
AMP-dependent protein kinase
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activity increases 2-3fold in presence of MgATP2-, activation is reversible, stimulation is optimal with 30 U/ml protein kinase
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.067 - 0.274
acetyl-CoA
0.046
butyryl-CoA
-
-
0.02
hexanoyl-CoA
-
-
0.128
propionyl-CoA
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-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.048
1-methoxyphaseolidin
Rattus norvegicus
-
IC50: 0.048 mM
0.057
1-methoxyphaseolin
Rattus norvegicus
-
IC50: 0.057 mM
0.02
2-methoxy-5-methyl-3-tetradecanoyloxy-1,4-benzoquinone
Rattus norvegicus
-
pH 6.9, 37°C
0.003
3-decanoyloxy-2-methoxy-5-methyl-1,4-benzoquinone
Rattus norvegicus
-
pH 6.9, 37°C
0.043
4-(1-Naphthylvinyl)pyridine
Rattus norvegicus
-
IC50: 0.043 mM
0.019
6,8-diprenylgenistein
Rattus norvegicus
-
IC50: 0.019 mM
0.148
baicalein
Rattus norvegicus
-
IC50: 0.148 mM
0.2
chiyusaponin
Rattus norvegicus
-
IC50: 0.2 mM
0.15
honokiol
Rattus norvegicus
-
IC50: 0.15 mM
0.0077
licoricidin
Rattus norvegicus
-
IC50: 0.0077 mM
0.045
luteolin
Rattus norvegicus
-
IC50: 0.045 mM
0.15
magnolol
Rattus norvegicus
-
IC50: 0.15 mM
0.29
nordihydroguaiaretic acid
Rattus norvegicus
-
IC50: 0.29 mM
0.08
quercetin
Rattus norvegicus
-
IC50: 0.08 mM
0.183
tetradecanoic acid
Rattus norvegicus
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pH 6.9, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0002
0.0004
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liver specific activity is reduced in presence of dexamethasone and medroxyprogesterone
0.001
-
spleen specific activity is reduced in presence of dexamethasone
0.002
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kidney medulla
0.0021
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A23187 stimulated cell
0.0026
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bone marrow
0.0039
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lymph nodes
0.004
0.01
-
spleen microsome
additional information
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
assay at
8.4
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PCAT1_RAT
534
1
59762
Swiss-Prot
Mitochondrion (Reliability: 2)
PCAT2_RAT
544
1
59829
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29000
-
x * 29000, SDS-PAGE and affinity labelling experiments
800000
-
MW after gel filtration, aggregate of several proteins or protein aggregates coated with lipids
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 29000, SDS-PAGE and affinity labelling experiments
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
stable to lyophilization
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
solubilization in sodium deoxycholate, precipitation by ammonium sulfate, ultragel AcA-22 chromatography, DEAE-sepharose CL-6B chromatography, covalent chromatography with thiopropyl-sepharose 6B
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lee, T.C.; Malone, B.; Snyder, F.
A new de novo pathway for the formation of 1-alkyl-2-acetyl-sn-glycerols, precursors of platelet activating factor. Biochemical characterization of 1-alkyl-2-lyso-sn-glycero-3-P:acetyl-CoA acetyltransferase in rat spleen
J. Biol. Chem.
261
5373-5377
1986
Rattus norvegicus
Manually annotated by BRENDA team
Gomez-Cambronero, J.; Velasco, S.; Sanchez-Crespo, M.; Vivanco, F.; Mato, J.M.
Partial purification and characterization of 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine: acetyl-CoA acetyltransferase from rat spleen
Biochem. J.
237
439-445
1986
Canis lupus familiaris, Rattus norvegicus
Manually annotated by BRENDA team
Lee, T.C.
Biosynthesis of platelet activating factor. Substrate specificity of 1-alkyl-2-lyso-sn-glycero-3-phosphocholine:acetyl-CoA acetyltransferase in rat spleen microsomes
J. Biol. Chem.
260
10952-10955
1985
Rattus norvegicus
Manually annotated by BRENDA team
Wykle, R.L.; Malone, B.; Snyder, F.
Enzymatic synthesis of 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine, a hypotensive and platelet-aggregating lipid
J. Biol. Chem.
255
10256-10260
1980
Rattus norvegicus
Manually annotated by BRENDA team
Gomez-Cambronero, J.; Velasco, S.; Mato, J.M.; Sanchez-Crespo, M.
Modulation of lyso-platelet activating factor: acetyl-CoA acetyltransferase from rat splenic microsomes. The role of cyclic AMP-dependent protein kinase
Biochim. Biophys. Acta
845
516-519
1985
Rattus norvegicus
Manually annotated by BRENDA team
Gomez-Cambronero, J.; Nieto, M.L.; Mato, J.M.; Sanchez-Crespo, M.
Modulation of lyso-platelet-activating factor: acetyl-CoA acetyltransferase from rat splenic microsomes. The role of calcium ions
Biochim. Biophys. Acta
845
511-515
1985
Rattus norvegicus
Manually annotated by BRENDA team
Yamazaki, R.; Sugatani, J.; Fujii, I.; Kuroyanagi, M.; Umehara, K.; Ueno, A.; Suzuki, Y.; Miwa, M.
Development of a novel method for determination of acetyl-CoA:1-alkyl-sn-glycero-3-phosphocholine acetyltransferase activity and its application to screening for acetyltransferase inhibitors. Inhibition by magnolol and honokiol from Magnoliae cortex
Biochem. Pharmacol.
47
995-1006
1994
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Yanoshita, R.; Chang, H.W.; Son, K.H.; Kudo, I.; Samejina, Y.
Inhibition of lysoPAF acetyltransferase activity by flavonoids
Inflamm. Res.
45
546-549
1996
Rattus norvegicus
Manually annotated by BRENDA team
Nagumo, S.; Fukuju, A.; Takayama, M.; Nagai, M.; Yanoshita, R.; Samejima, Y.
Inhibition of lysoPAF acetyltransferase activity by components of licorice root
Biol. Pharm. Bull.
22
1144-1146
1999
Rattus norvegicus
Manually annotated by BRENDA team
Ihara, Y.; Frenkel, R.A.; Johnston, J.M.
Hormonal regulation of platelet-activating factor-acetyltransferase activity in rat tissues
Arch. Biochem. Biophys.
304
503-507
1993
Rattus norvegicus
Manually annotated by BRENDA team
Yamazaki, Y.; Yasuda, K.; Matsuyama, T.; Ishihara, T.; Higa, R.; Sawairi, T.; Yamaguchi, M.; Egi, M.; Akai, S.; Miyase, T.; Ikari, A.; Miwa, M.; Sugatani, J.
A Penicillium sp. F33 metabolite and its synthetic derivatives inhibit acetyl-CoA:1-O-alkyl-sn-glycero-3-phosphocholine acetyltransferase (a key enzyme in platelet-activating factor biosynthesis) and carrageenan-induced paw edema in mice
Biochem. Pharmacol.
86
632-644
2013
Mus musculus, Mus musculus (Q8BYI6), Rattus norvegicus
Manually annotated by BRENDA team