Reference on EC 2.3.1.54 - formate C-acetyltransferase
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Weidner, G.; Sawers, G.
Molecular characterization of the genes encoding pyruvate formate-lyase and its activating enzyme of Clostridium pasteurianum
J. Bacteriol.
178
2440-2444
1996
Clostridium butyricum, Clostridium kluyveri, Clostridium pasteurianum, Clostridium pasteurianum (Q46266), Clostridium sp., Escherichia coli, Enterococcus faecalis, no activity in Clostridium pasteurianum, Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguinis, Clostridium pasteurianum DSM 525, Clostridium butyricum DSM 552, Streptococcus sanguinis DSM 20066
Kulzer, R.; Pils, T.; Kappl, R.; Huttermann, J.; Knappe, J.
Reconstitution and characterization of the polynuclear iron-sulfur cluster in pyruvate formate-lyase-activating enzyme. Molecular properties of the holoenzyme form
J. Biol. Chem.
273
4897-4903
1998
Escherichia coli
Conradt, H.; Hohmann-Berger, M.; Hohmann, H.P.; Blaschkowski, H.P.; Knappe, J.
Pyruvate formate-lyase (inactive form) and pyruvate formate-lyase activating enzyme of Escherichia coli: isolation and structural properties
Arch. Biochem. Biophys.
228
133-142
1984
Escherichia coli, Streptococcus mutans
Rdel, W.; Plaga, W.; Frank, W.; Knappe, J.
Primary structures of Escherichia coli pyruvate formate-lyase and pyruvate-formate-lyase-activating enzyme deduced from the DNA nucleotide sequences
Eur. J. Biochem.
177
153-158
1988
Escherichia coli
Thauer, R.K.; Kirchniawy, F.H.; Jungermann, K.A.
Properties and function of the pyruvate-formate-lyase reaction in clostridiae
Eur. J. Biochem.
27
282-290
1972
Clostridium beijerinckii, Clostridium butyricum, Clostridium kluyveri, Clostridium sp., Escherichia coli, Enterococcus faecalis, Veillonella parvula, no activity in Clostridium pasteurianum
Wood, N.P.; Jungermann, K.
Inactivation of the pyruvate formate lyase of Clostridium butyricum
FEBS Lett.
27
49-52
1972
Clostridium butyricum, Escherichia coli, Enterococcus faecalis
Knappe, J.; Balschkowski, H.P.; Grbner, P.; Schmitt, T.
Pyruvate formate-lyase of Escherichia coli: the acetyl-enzyme intermediate
Eur. J. Biochem.
50
253-263
1974
Escherichia coli
Knappe, J.; Blaschkowski, H.P.
Pyruvate formate-lyase from Escherichia coli and its activation system
Methods Enzymol.
41B
508-518
1975
Clostridium sp., Escherichia coli, Enterococcus faecalis
-
Takahashi, S.; Abbe, K.; Yamada, T.
Purification of pyruvate formate-lyase from Streptococcus mutans and its regulatory properties
J. Bacteriol.
149
1034-1040
1982
Clostridium butyricum, Clostridium sp., Escherichia coli, Enterococcus faecalis, Lactobacillus delbrueckii subsp. bulgaricus, Lactococcus lactis, Streptococcus mutans, Streptococcus mutans JC2
Ulissi-DeMario, L.; Brush, E.J.; Kozarich, J.W.
Mechanism-based inactivation of pyruvate formate-lyase reaction in Clostridiae
J. Am. Chem. Soc.
113
4341-4342
1991
Escherichia coli
-
Wagner, A.F.V.; Frey, M.; Neugebauer, F.A.; Schfer, W.; Knappe, J.
The free radical in pyruvate formate-lyase is located on glycine-734
Proc. Natl. Acad. Sci. USA
89
996-1000
1992
Escherichia coli
Knappe, J.; Elbert, S.; Frey, M.; Wagner, A.F.V.
Pyruvate formate-lyase mechanism involving the protein-based glycyl radical
Biochem. Soc. Trans.
21
731-734
1993
Escherichia coli
-
Kaiser, M.; Sawers, G.
Pyruvate formate-lyase is not essential for nitrate respiration by Escherichia coli
FEMS Microbiol. Lett.
117
163-168
1994
Escherichia coli
Broderick, J.B.; Duderstadt, R.E.; Fernandez, D.C.; Wojtuszewski, K.; Henshaw, T.F.; Johnson, M.K.
Pyruvate formate-lyase activating enzyme is an iron-sulfur protein
J. Am. Chem. Soc.
119
7396-7397
1997
Escherichia coli
-
Himo, F.; Eriksson, L.A.
Catalytic mechanism of pyruvate formate-lyase (PFL). A theoretical study
J. Am. Chem. Soc.
120
11449-11455
1998
Escherichia coli
-
Becker, A.; Fritz-Wolf, K.; Kabsch, W.; Knappe, J.; Schultz, S.; Wagner, A.F.V.
Structure and mechanism of the glycyl radical enzyme pyruvate formate-lyase
Nat. Struct. Biol.
6
969-975
1999
Escherichia coli, Escherichia coli (P09373)
Asanuma, N.; Hino, T.
Effects of pH and energy supply on activity and amount of pyruvate formate-lyase in Streptococcus bovis
Appl. Environ. Microbiol.
66
3773-3777
2000
Clostridium butyricum, Escherichia coli, Lactococcus lactis, Streptococcus equinus, Streptococcus mutans
Wagner, A.F.V.; Schultz, S.; Bomke, J.; Pils, T.; Lehmann, W.D.; Knappe, J.
YfiD of Escherichia coli and Y06I of bacteriophage T4 as autonomous glycyl radical cofactors reconstituting the catalytic center of oxygen-fragmented pyruvate formate-lyase
Biochem. Biophys. Res. Commun.
285
456-462
2001
Tequatrovirus T4, Escherichia coli, Escherichia coli (P09373), Haemophilus influenzae, Pasteurella multocida, Yersinia pestis, Salmonella enterica subsp. enterica serovar Typhimurium, Vibrio cholerae serotype O1
Zhang, W.; Wong, K.K.; Magliozzo, R.S.; Kozarich, J.W.
Inactivation of pyruvate formate-lyase by dioxygen: Defining the mechanistic interplay of glycine 734 and cysteine 419 by rapid freeze-quench EPR
Biochemistry
40
4123-4130
2001
Escherichia coli
Asanuma, N.; Hino, T.
Molecular characterization and expression of pyruvate formate-lyase-activating enzyme in a ruminal bacterium, Streptococcus bovis
Appl. Environ. Microbiol.
68
3352-3357
2002
Clostridium pasteurianum, Escherichia coli, Lactococcus lactis, Streptococcus equinus, Streptococcus equinus (O66391)
Becker, A.; Kabsch, W.
X-ray structure of pyruvate formate-lyase in complex with pyruvate and CoA. How the enzyme uses the Cys-418 thiyl radical for pyruvate cleavage
J. Biol. Chem.
277
40036-40042
2002
Escherichia coli (P09373)
Lehtioe, L.; Leppaenen, V.M.; Kozarich, J.W.; Goldman, A.
Structure of Escherichia coli pyruvate formate-lyase with pyruvate
Acta Crystallogr. Sect. D
58
2209-2212
2002
Escherichia coli (P09373), Escherichia coli
Melchiorsen, C.R.; Jokumsen, K.V.; Villadsen, J.; Israelsen, H.; Arnau, J.
The level of pyruvate-formate lyase controls the shift from homolactic to mixed-acid product formation in Lactococcus lactis
Appl. Microbiol. Biotechnol.
58
338-344
2002
Escherichia coli, Lactococcus lactis
Hasona, A.; Kim, Y.; Healy, F.G.; Ingram, L.O.; Shanmugam, K.T.
Pyruvate formate lyase and acetate kinase are essential for anaerobic growth of Escherichia coli on xylose
J. Bacteriol.
186
7593-7600
2004
Escherichia coli
Plaga, W.; Vielhaber, G.; Wallach, J.; Knappe, J.
Modification of Cys-418 of pyruvate format-lyase by methacrylic acid, based on its radical mechanism
FEBS Lett.
466
45-48
2000
Escherichia coli
Gelius-Dietrich, G.; Henze, K.
Pyruvate formate lyase (PFL) and PFL activating enzyme in the chytrid fungus Neocallimastix frontalis: a free-radical enzyme system conserved across divergent eukaryotic lineages
J. Eukaryot. Microbiol.
51
456-463
2004
Neocallimastix frontalis (Q6RFH7), Neocallimastix frontalis
De Lucas, M.; Fernandes, P.A.; Eriksson, L.A.; Ramos, M.J.
Pyruvate formate lyase: a new perspective
J. Phys. Chem. B
107
5751-5757
2003
Escherichia coli
-
Boxma, B.; Voncken, F.; Jannink, S.; van Alen, T.; Akhmanova, A.; van Weelden, S.W.; van Hellemond, J.J.; Ricard, G.; Huynen, M.; Tielens, A.G.; Hackstein, J.H.
The anaerobic chytridiomycete fungus Piromyces sp. E2 produces ethanol via pyruvate:formate lyase and an alcohol dehydrogenase E
Mol. Microbiol.
51
1389-1399
2004
Piromyces sp. E2
Takahashi-Abbe, S.; Abe, K.; Takahashi, N.
Biochemical and functional properties of a pyruvate formate-lyase (PFL)-activating system in Streptococcus mutans
Oral Microbiol. Immunol.
18
293-297
2003
Streptococcus mutans
Derzelle, S.; Bolotin, A.; Mistou, M.Y.; Rul, F.
Proteome analysis of Streptococcus thermophilus grown in milk reveals pyruvate formate-lyase as the major upregulated protein
Appl. Environ. Microbiol.
71
8597-8605
2005
Streptococcus thermophilus (Q5M2Y4), Streptococcus thermophilus, Streptococcus thermophilus LMG18311 (Q5M2Y4)
Meshulam-Simon, G.; Behrens, S.; Choo, A.D.; Spormann, A.M.
Hydrogen metabolism in Shewanella oneidensis MR-1
Appl. Environ. Microbiol.
73
1153-1165
2007
Shewanella oneidensis, Shewanella oneidensis MR-1 / ATCC 700550
Nnyepi, M.R.; Peng, Y.; Broderick, J.B.
Inactivation of E. coli pyruvate formate-lyase: role of AdhE and small molecules
Arch. Biochem. Biophys.
459
1-9
2007
Escherichia coli
Yang, D.F.; Wei, Y.T.; Huang, R.B.
Computer-aided design of the stability of pyruvate formate-lyase from Escherichia coli by site-directed mutagenesis
Biosci. Biotechnol. Biochem.
71
746-753
2007
Escherichia coli, Escherichia coli JM109
Zhu, J.; Shalel-Levanon, S.; Bennett, G.; San, K.Y.
The YfiD protein contributes to the pyruvate formate-lyase flux in an Escherichia coli arcA mutant strain
Biotechnol. Bioeng.
97
138-143
2007
Escherichia coli
Sparling, R.; Islam, R.; Cicek, N.; Carere, C.; Chow, H.; Levin, D.B.
Formate synthesis by Clostridium thermocellum during anaerobic fermentation
Can. J. Microbiol.
52
681-688
2006
Acetivibrio thermocellus
Lucas, M.d.e.F.; Ramos, M.J.
Theoretical study of the suicide inhibition mechanism of the enzyme pyruvate formate lyase by methacrylate
J. Am. Chem. Soc.
127
6902-6909
2005
Escherichia coli
Atteia, A.; van Lis, R.; Gelius-Dietrich, G.; Adrait, A.; Garin, J.; Joyard, J.; Rolland, N.; Martin, W.
Pyruvate formate-lyase and a novel route of eukaryotic ATP synthesis in Chlamydomonas mitochondria
J. Biol. Chem.
281
9909-9918
2006
Chlamydomonas reinhardtii (Q1RS83), Chlamydomonas reinhardtii
Lehtioe, L.; Grossmann, J.G.; Kokona, B.; Fairman, R.; Goldman, A.
Crystal structure of a glycyl radical enzyme from Archaeoglobus fulgidus
J. Mol. Biol.
357
221-235
2006
Archaeoglobus fulgidus
Hemschemeier, A.; Jacobs, J.; Happe, T.
Biochemical and physiological characterization of the pyruvate formate-lyase Pfl1 of Chlamydomonas reinhardtii, a typically bacterial enzyme in a eukaryotic alga
Eukaryot. Cell
7
518-526
2008
Chlamydomonas reinhardtii (P37836), Chlamydomonas reinhardtii
Condic-Jurkic, K.; Perchyonok, V.T.; Zipse, H.; Smith, D.M.
On the modeling of arginine-bound carboxylates: A case study with Pyruvate Formate-Lyase
J. Comput. Chem.
29
2425-2433
2008
Escherichia coli (P09373)
Wang, Q.; Ou, M.S.; Kim, Y.; Ingram, L.O.; Shanmugam, K.T.
Metabolic Flux Control at the Pyruvate Node in an Anaerobic Escherichia coli with Active Pyruvate Dehydrogenase
Appl. Environ. Microbiol.
76
2107-2114
2010
Escherichia coli str. K-12 substr. W3110
Yesilkaya, H.; Spissu, F.; Carvalho, S.M.; Terra, V.S.; Homer, K.A.; Benisty, R.; Porat, N.; Neves, A.R.; Andrew, P.W.
Pyruvate formate lyase is required for pneumococcal fermentative metabolism and virulence
Infect. Immun.
77
5418-5427
2009
Streptococcus pneumoniae D39
Utrilla, J.; Gosset, G.; Martinez, A.
ATP limitation in a pyruvate formate lyase mutant of Escherichia coli MG1655 increases glycolytic flux to D-lactate
J. Ind. Microbiol. Biotechnol.
36
1057-1062
2009
Escherichia coli str. K-12 substr. MG1655
Leibig, M.; Liebeke, M.; Mader, D.; Lalk, M.; Peschel, A.; Goetz, F.
Pyruvate formate lyase acts as a formate supplier for metabolic processes during anaerobiosis in Staphylococcus aureus
J. Bacteriol.
193
952-962
2011
Staphylococcus aureus
Crain, A.V.; Broderick, J.B.
Pyruvate formate-lyase and its activation by pyruvate formate-lyase activating enzyme
J. Biol. Chem.
289
5723-5729
2013
Escherichia coli (P09373)
Zhou, J.; Olson, D.; Lanahan, A.; Tian, L.; Murphy, S.; Lo, J.; Lynd, L.
Physiological roles of pyruvate ferredoxin oxidoreductase and pyruvate formate-lyase in Thermoanaerobacterium saccharolyticum JW/SL-YS485
Biotechnol. Biofuels
8
138
2015
Thermoanaerobacterium saccharolyticum (I3VT07), Thermoanaerobacterium saccharolyticum, Thermoanaerobacterium saccharolyticum DSM 8691 (I3VT07)
Zhang, Y.; Dai, Z.; Krivoruchko, A.; Chen, Y.; Siewers, V.; Nielsen, J.
Functional pyruvate formate lyase pathway expressed with two different electron donors in Saccharomyces cerevisiae at aerobic growth
FEMS Yeast Res.
15
fov024
2015
Escherichia coli (P09373), Escherichia coli
Khelifi, N.; Amin Ali, O.; Roche, P.; Grossi, V.; Brochier-Armanet, C.; Valette, O.; Ollivier, B.; Dolla, A.; Hirschler-Rea, A.
Anaerobic oxidation of long-chain n-alkanes by the hyperthermophilic sulfate-reducing archaeon, Archaeoglobus fulgidus
ISME J.
8
2153-2166
2014
Archaeoglobus fulgidus (O28823), Archaeoglobus fulgidus
Doi, Y.; Ikegami, Y.
Pyruvate formate-lyase is essential for fumarate-independent anaerobic glycerol utilization in the Enterococcus faecalis strain W11
J. Bacteriol.
196
2472-2480
2014
Enterococcus faecalis, Enterococcus faecalis W11
Doberenz, C.; Zorn, M.; Falke, D.; Nannemann, D.; Hunger, D.; Beyer, L.; Ihling, C.H.; Meiler, J.; Sinz, A.; Sawers, R.G.
Pyruvate formate-lyase interacts directly with the formate channel FocA to regulate formate translocation
J. Mol. Biol.
426
2827-2839
2014
Escherichia coli (P09373), Escherichia coli
Zhang, Y.; Dai, Z.; Krivoruchko, A.; Chen, Y.; Siewers, V.; Nielsen, J.
Functional pyruvate formate lyase pathway expressed with two different electron donors in Saccharomyces cerevisiae at aerobic growth
FEMS Yeast Res.
15
fov024
2015
Escherichia coli (P09373), Escherichia coli
Crain, A.; Broderick, J.
Pyruvate formate-lyase and its activation by pyruvate formate-lyase activating enzyme
J. Biol. Chem.
289
5723-5729
2014
Escherichia coli (P09373)
Al-Bayati, F.A.; Kahya, H.F.; Damianou, A.; Shafeeq, S.; Kuipers, O.P.; Andrew, P.W.; Yesilkaya, H.
Pneumococcal galactose catabolism is controlled by multiple regulators acting on pyruvate formate lyase
Sci. Rep.
7
43587
2017
Streptococcus pneumoniae (A0A0H2ZQE4), Streptococcus pneumoniae
Zelcbuch, L.; Lindner, S.N.; Zegman, Y.; Vainberg Slutskin, I.; Antonovsky, N.; Gleizer, S.; Milo, R.; Bar-Even, A.
Pyruvate formate-lyase enables efficient growth of Escherichia coli on acetate and formate
Biochemistry
55
2423-2426
2016
Escherichia coli
Hanzevacki, M.; Condic-Jurkic, K.; Banhatti, R.D.; Smith, A.S.; Smith, D.M.
The influence of chemical change on protein dynamics a case study with pyruvate formate-lyase
Chemistry
25
8741-8753
2019
Escherichia coli (P09373)
Hanzevacki, M.; Banhatti, R.D.; Condic-Jurkic, K.; Smith, A.S.; Smith, D.M.
Exploring reactive conformations of coenzyme A during binding and unbinding to pyruvate formate-lyase
J. Phys. Chem. A
123
9345-9356
2019
Escherichia coli (P09373)
Kim, S.; Lee, J.; Lee, S.; Ha, J.; Lee, J.; Choi, Y.; Oh, H.; Yoon, Y.; Choi, K.
Invasion of intestinal cells by Staphylococcus aureus is mediated by pyruvate formate lyase (Pfl) protein
J. Pure Appl. Microbiol.
13
647-652
2019
Staphylococcus aureus, Staphylococcus aureus NCCP10862, Staphylococcus aureus KACC10768
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