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Information on EC 2.3.1.51 - 1-acylglycerol-3-phosphate O-acyltransferase and Organism(s) Bos taurus

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EC Tree
IUBMB Comments
Acyl-[acyl-carrier protein] can also act as an acyl donor. The animal enzyme is specific for the transfer of unsaturated fatty acyl groups.
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This record set is specific for:
Bos taurus
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Word Map
The taxonomic range for the selected organisms is: Bos taurus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
abhd5, agpat2, lpaat, lysophosphatidic acid acyltransferase, lpaat-beta, agpat1, 1-acylglycerol-3-phosphate o-acyltransferase, agpat3, lpa acyltransferase, agpat4, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1-acyl-sn-glycero-3-phosphate acyltransferase
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1-acyl-sn-glycerol 3-phosphate acyltransferase
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1-acylglycero-3-phosphate acyltransferase
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1-acylglycerolphosphate acyltransferase
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1-acylglycerophosphate acyltransferase
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acyltransferase, 1-acylglycerol phosphate
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lysophosphatidic acid-acyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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PATHWAY SOURCE
PATHWAYS
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-, -, -, -, -, -, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
acyl-CoA:1-acyl-sn-glycerol-3-phosphate 2-O-acyltransferase
Acyl-[acyl-carrier protein] can also act as an acyl donor. The animal enzyme is specific for the transfer of unsaturated fatty acyl groups.
CAS REGISTRY NUMBER
COMMENTARY hide
51901-16-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
the enzyme is active towards acyl-CoAs of chain length C-8 to C-18
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?
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
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-
-
?
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
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-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
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maximum activity is obtained with palmitoyl-CoA at a concentration of 0.002-0.003 mM
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?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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1-palmitoyl- and 1-oleoyl-sn-glycerol 3-phosphate are probably the substrates for the enzyme in vivo, since palmitic and oleic acids are the major fatty acid components at the sn-1 position of cow milk triacylglycerols. The specificity of the enzyme plays a major role in determining the fatty acid composition at the sn-2 position of cow milk triacylglycerols, though the influence of other factors such as the in vivo concentration of the acyl-CoAs and the specificities of the monoacylglycerol acyltransferases cannot be ruled out
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?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
stearoyl-CoA
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strongly inhibitory at 0.002 mM
additional information
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the enzyme can utilize the monomer form of long-chain acyl-CoA but it is inhibited by the micellar form of these substrates
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Jersey cow
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-
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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lactating
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
-
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Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PLCD_BOVIN
378
3
43907
Swiss-Prot
Secretory Pathway (Reliability: 1)
PLCA_BOVIN
287
3
32005
Swiss-Prot
Secretory Pathway (Reliability: 1)
Q1RMV6_BOVIN
278
4
31374
TrEMBL
Secretory Pathway (Reliability: 1)
F1MZS9_BOVIN
278
4
31358
TrEMBL
Secretory Pathway (Reliability: 1)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Marshall, M.O.; Knudsen, J.
The specificity of 1-acyl-sn-glycerol 3-phosphate acyltransferase in microsomal fractions from lactating cow mammary gland towards short, medium and long chain acyl-CoA esters
Biochim. Biophys. Acta
489
236-241
1977
Bos taurus
Manually annotated by BRENDA team