Information on EC 2.3.1.41 - beta-ketoacyl-[acyl-carrier-protein] synthase I

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.3.1.41
-
RECOMMENDED NAME
GeneOntology No.
beta-ketoacyl-[acyl-carrier-protein] synthase I
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
show the reaction diagram
mechanism
-
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
show the reaction diagram
structure
-
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
show the reaction diagram
acetylation of the synthase III-like polyketid synthase at Ser118
-
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
show the reaction diagram
active site cysteine
-
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
show the reaction diagram
active site cysteine
-
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
show the reaction diagram
synthaseIII/FabH: His-Asn-Cys catalytic triad
-
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
show the reaction diagram
synthaseIII/FabH: His-Asn-Cys catalytic triad
P0A3C5
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
show the reaction diagram
active site cysteine; structure of active site, substrate binding pocket, and subunit structure in relation to substrate specificity of the 3 isozyme types
-
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
show the reaction diagram
synthaseII/FabB: His-HIs-Cys catalytic triad; synthaseIII/FabH: His-Asn-Cys catalytic triad
-
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
show the reaction diagram
mechanism and active site structure and function
-
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
show the reaction diagram
active site cysteine; mechanism
-
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
show the reaction diagram
active site cysteine
-
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
show the reaction diagram
mechanism, roles of H303, H337, C164
-
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
show the reaction diagram
catalytic active site residues are Cys163 and His333, the active site contains two oxyanion holes, stabilizing the thioester oxyanion of acetylated CoA and of acetylated Cys92, respectively
P0A953
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
show the reaction diagram
comparison of active sites in isoforms KAS I, KAS II and related enzymes, geometry and catalytic role of equivalent residues
-
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
show the reaction diagram
beta-ketoacyl-acyl-carrier protein synthase enzyme joins short carbon units to construct fatty acyl chains by a three-step Claisen condensation reaction, the reaction starts with a trans thioesterification of the acyl primer substrate from ACP to the enzyme, subsequently, the donor substrate malonyl-ACP is decarboxylated to form a carbanion intermediate, which in the third step attacks C1 of the primer substrate giving rise to an elongated acyl chain, H298 serves as a catalytic base in the decarboxylation step, the enzyme possesses a Cys-His-His catalytic triad, Lys328 has a dual role in catalysis: its charge influences acyl transfer to the active site Cys, and the steric restraint imposed on H333, as an obligate hydrogen bond donor at Ne, is of critical importance for decarboxylation activity, active sites of the wild-type KAS I, its H298 mutants, and their acyl complexes, overview
-
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
show the reaction diagram
structure-activity analysis, reaction mechanism
-, Q9NWU1
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Acyl group transfer
-
-
-
-
condensation
-
-
condensation
-
-
condensation
-
-
condensation
P0A3C5
-
condensation
-
-
condensation
-
-
condensation
P99159
-
condensation
-
-
decarboxylation
-
-
decarboxylation
-
-
decarboxylation
-
-
decarboxylation
P99159
-
PATHWAY
KEGG Link
MetaCyc Link
8-amino-7-oxononanoate biosynthesis I
-
Biotin metabolism
-
Fatty acid biosynthesis
-
fatty acid biosynthesis initiation II
-
fatty acid biosynthesis initiation III
-
fatty acid elongation -- saturated
-
Metabolic pathways
-
mycolate biosynthesis
-
octanoyl-ACP biosynthesis (mitochondria, yeast)
-
palmitate biosynthesis I (animals and fungi)
-
palmitate biosynthesis II (bacteria and plants)
-
palmitoleate biosynthesis I
-
stearate biosynthesis II (bacteria and plants)
-
stearate biosynthesis III (fungi)
-
superpathway of fatty acid biosynthesis initiation (E. coli)
-
SYSTEMATIC NAME
IUBMB Comments
acyl-[acyl-carrier protein]:malonyl-[acyl-carrier protein] C-acyltransferase (decarboxylating)
This enzyme is responsible for the chain-elongation step of dissociated (type II) fatty-acid biosynthesis, i.e. the addition of two C atoms to the fatty-acid chain. Escherichia coli mutants that lack this enzyme are deficient in unsaturated fatty acids. The enzyme can use fatty acyl thioesters of ACP (C2 to C16) as substrates, as well as fatty acyl thioesters of Co-A (C4 to C16) [4]. The substrate specificity is very similar to that of EC 2.3.1.179, beta-ketoacyl-ACP synthase II, with the exception that the latter enzyme is far more active with palmitoleoyl-ACP (C16Delta9) as substrate, allowing the organism to regulate its fatty-acid composition with changes in temperature [4,5].
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3-ketoacyl-ACP synthase
-
-
3-ketoacyl-ACP synthase
-
-
3-ketoacyl-acyl carrier protein synthase
-
-
-
-
3-oxoacyl synthase
-
-
3-oxoacyl-[acyl-carrier-protein] synthase
-
-
-
-
acyl-malonyl(acyl-carrier-protein)-condensing enzyme
-
-
-
-
beta-ketoacyl ACP synthase I
-
-
beta-ketoacyl acyl carrier protein synthase
-
-
-
-
beta-ketoacyl synthase
-
-
beta-ketoacyl synthetase
-
-
-
-
beta-ketoacyl [ACP] synthase
Q9NWU1
-
beta-ketoacyl-ACP synthase
Q9NWU1
-
beta-ketoacyl-ACP synthase
Q586W9
-
beta-ketoacyl-ACP synthase I
-
-
beta-ketoacyl-ACP synthase I
-
-
beta-ketoacyl-ACP synthase I
-
-
beta-ketoacyl-ACP synthetase
-
-
-
-
beta-ketoacyl-ACP-synthase
Q8L3X9
-
beta-ketoacyl-acyl carrier protein synthase I
-
-
beta-ketoacyl-acyl carrier protein synthetase
-
-
-
-
beta-ketoacyl-CoA synthase-I
-
-
beta-ketoacyl-[acyl carrier protein (ACP)] synthase III
-
-
beta-ketoacyl-[acyl carrier protein] synthase
-
-
-
-
beta-ketoacyl-[acyl-carrier-protein] synthase I
P52410
-
beta-ketoacyl-[acyl-carrier-protein] synthase I
-
-
beta-ketoacylsynthase
-
-
-
-
condensing enzyme
-
-
-
-
Cys-His-His-type beta-ketoacyl-acyl carrier protein synthase
-
-
elongase
Q586W9
-
elongating beta-ketoacyl-acyl carrier protein synthase
-
-
fatty acid condensing enzyme
-
-
-
-
KAS
Q9NWU1
-
synthase, 3-oxoacyl-[acyl-carrier-protein]
-
-
-
-
additional information
-
in vertebrates, yeast and Mycobacteria beta-ketoacyl synthase activity is a domain of the multifunctional polypeptide chains of fatty acid synthase EC 2.3.1.85 and EC 2.3.1.86
additional information
-
in vertebrates, yeast and Mycobacteria beta-ketoacyl synthase activity is a domain of the multifunctional polypeptide chains of fatty acid synthase EC 2.3.1.85 and EC 2.3.1.86
additional information
-
the enzyme belongs to the superfamily of condensing enzymes which includes thiolases
additional information
-
enzyme shows also acetyl coenzyme A-acyl-carrier-protein transacylase activity EC 2.3.1.38, 12% compared to beta-keto-[acyl-carrier-protein] synthase activity EC 2.3.1.41
additional information
-
gene DpsC, encoding apolyketide synthase, exhibits also beta-keto-[acyl-carrier-protein] synthase III activity, which is restricted for propionyl-[acyl-carrier-protein] as the only [acyl-carrier-protein]-substrate
additional information
-
3 structural classes of condensing enzymes
CAS REGISTRY NUMBER
COMMENTARY
9077-10-5
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
synthase II
-
-
Manually annotated by BRENDA team
expression in Escherichia coli
Swissprot
Manually annotated by BRENDA team
genes fabH1 and fabH2, 2 synthase III isozymes
-
-
Manually annotated by BRENDA team
cv. Westar
-
-
Manually annotated by BRENDA team
synthase I and II
-
-
Manually annotated by BRENDA team
seed plastid-specific isoform with similarities to synthase I type
-
-
Manually annotated by BRENDA team
synthase IV
-
-
Manually annotated by BRENDA team
B; synthases I and II
-
-
Manually annotated by BRENDA team
gene fabB
-
-
Manually annotated by BRENDA team
K12 strains UC1, WN1 is a fabF-mutant lacking synthase II, and various mutants, overview; synthases I and II
-
-
Manually annotated by BRENDA team
synthase I
-
-
Manually annotated by BRENDA team
synthase III, gene fabH
-
-
Manually annotated by BRENDA team
synthases I, II, and III
-
-
Manually annotated by BRENDA team
Euglena gracilis Z
Z
-
-
Manually annotated by BRENDA team
expression in Escherichia coli, without N-terminal targeting sequence
-
-
Manually annotated by BRENDA team
the human beta-ketoacyl [ACP] synthase from the mitochondrial type II fatty acid synthase
SwissProt
Manually annotated by BRENDA team
cv. Bonus
-
-
Manually annotated by BRENDA team
sp. lactis IL1403
-
-
Manually annotated by BRENDA team
synthase I, i.e. KasA
SwissProt
Manually annotated by BRENDA team
synthase II, i.e. KasB
-
-
Manually annotated by BRENDA team
FabF/FabH; strain 3D7, gene fabBF, encoding the only elongating beta-ketoacyl-ACP synthase in the organism
-
-
Manually annotated by BRENDA team
synthase I and II
-
-
Manually annotated by BRENDA team
synthase III
-
-
Manually annotated by BRENDA team
methicillin-resistant and vancomycin-resistant strains
-
-
Manually annotated by BRENDA team
strain ATCC 35556; synthase III, gene fabH
GenBank
Manually annotated by BRENDA team
synthase III, gene fabH
SwissProt
Manually annotated by BRENDA team
synthase III, gene fabH
-
-
Manually annotated by BRENDA team
synthase III-like activity
-
-
Manually annotated by BRENDA team
isozyme II
-
-
Manually annotated by BRENDA team
synthase II
-
-
Manually annotated by BRENDA team
mitochondrial beta-ketoacyl synthase; ssp. brucei, strain 427, procyclic trypanosomes
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
-
FabB catalyzes the elongation of cis-3-decenoyl-ACP produced by FabA to generate the first 12-carbon cis-unsaturated fatty acid intermediate, 3-oxo-cis-5-decenoyl-ACP, which is converted to cis-5-dodecenoyl-ACP. FabB is essential in the unsaturated fatty acid, UFA, synthesis pathway, strains lacking FabB are UFA auxotroph, overview. FabB cannot be substituted by FabF
physiological function
P52410
crucial for fatty acid synthesis, deficiency results in semidwarf and variegated leaves, in a mutant chloroplast division is arrested at early developmental stages of rosette leaves
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxo-6-octadecenoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
best substrate, highly specific for, reaction is part of petroselinic acid biosynthesis, synthase IV
-
-
?
acetyl-CoA + malonyl-CoA
3-oxobutyryl-CoA + CoA
show the reaction diagram
-
-
formation of about 1% triacetic acid lactone
-
?
acetyl-CoA + malonyl-[acyl-carrier protein]
acetoacyl-[acyl-carrier protein] + CO2 + CoA
show the reaction diagram
-
-
-
?
acetyl-CoA + malonyl-[acyl-carrier protein]
acetoacyl-[acyl-carrier protein] + CO2 + CoA
show the reaction diagram
P0A3C5
synthase III, best substrate
-
?
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
ir
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
r
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
r
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
?
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
synthase III
-
-
?
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
P99159
synthase III
-
ir
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
synthase III, recombinant enzyme from E. coli is absolutely specific for
-
r
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
synthase I
-
-
?
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-, P63454
Kas A and KasB
-
-
?
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxobutanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
acyl-CoA + [acyl-carrier protein]
acyl-[acyl-carrier protein] + CoA
show the reaction diagram
-
tetradecanoyl-CoA and cis-9-hexadecenoyl-CoA are poor substrates
-
r
acyl-CoA + [acyl-carrier protein]
acyl-[acyl-carrier protein] + CoA
show the reaction diagram
-
catalyzes fatty acyl transfer between CoA and [acyl-carrier-protein], but no direct transfer to the enzyme as with acyl-[acyl-carrier-protein]
-
-
?
acyl-CoA + [acyl-carrier protein]
acyl-[acyl-carrier protein] + CoA
show the reaction diagram
-
catalyzes fatty acyl transfer between CoA and [acyl-carrier-protein], but no direct transfer to the enzyme as with acyl-[acyl-carrier-protein]
-
r
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
Q8L3X9
-
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
part of non-associated fatty acid synthase system of plants and prokaryotes
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
part of non-associated fatty acid synthase system of plants and prokaryotes
-
-
ir
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
part of non-associated fatty acid synthase system of plants and prokaryotes
-
-
r
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
part of non-associated fatty acid synthase system of plants and prokaryotes
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
part of non-associated fatty acid synthase system of plants and prokaryotes
-
-
-
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
involved in biosynthesis of saturated fatty acids
-
-
ir
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
involved in biosynthesis of saturated fatty acids
-
-
r
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
involved in biosynthesis of saturated fatty acids
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
involved in biosynthesis of saturated fatty acids
-
-
r
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
key enzyme for regulation of medium-chain fatty acid synthesis in seeds
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
P99159
initiation of fatty acid biosynthesis, part of fatty acid synthase II
-
-
ir
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
involved in biosynthesis of unsaturated and saturated fatty acids
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-, P63454
part of fatty acid synthase system I and II, involved in cell wall mycolic acids biosynthesis, overview
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
initiation of fatty acid biosynthesis with acetyl-CoA as a primer, overview substrate specificity
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
responsible for chain elongation during de novo fatty acid synthesis
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
responsible for chain elongation during de novo fatty acid synthesis
-
-
-
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
involved in biosynthesis of mono-unsaturated long-chain fatty acids
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
initiation of both straight- and branched-chain fatty acid biosynthesis resulting in a wide variety of produced fatty acids in vivo
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
acyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-, C4 to C14 acyl-[ACP]s, the enzyme readily elongates C4:0-through C10:0-PfACPs, has significantly less activity with C12:0- and C14:0-ACPs, and no capacity for elongation of C16:0-ACP and C18:0-ACP
-
-
?
an acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
a 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
the endoplasmic reticulum enzyme is involved in synthesis of very long chain fatty acids, KCS-I displays a preference for 20:0-CoA, substrate specificity, overview
-
-
?
butanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxohexanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
butyryl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
beta-ketohexanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
ir
butyryl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
beta-ketohexanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
synthase III
-
-
?
butyryl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
beta-ketohexanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
P99159
synthase III
-
-
?
C10-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
C12-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
C12-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
C14-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
C14-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
C16-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
C4-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
C6-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
C6-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
C8-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
best substrate
-
-
?
C8-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
C10-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
cis-3-decenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxo-cis-5-dodecenoyl-[acyl-carrier protein] + acyl-carrier protein
show the reaction diagram
-
-
-
-
-
cis-3-decenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxo-cis-5-dodecenoyl-[acyl-carrier protein] + acyl-carrier protein
show the reaction diagram
-
-
-
-
?
cis-3-decenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxo-cis-5-dodecenoyl-[acyl-carrier protein] + acyl-carrier protein
show the reaction diagram
-
key step in the unsaturated fatty acid, UFA, synthesis pathway. FabB is negatively regulated by the FabR protein
the product is not incorporated into the complex lipids of the bacterium
-
?
cis-9-hexadecenoyl-CoA + malonyl-[acyl-carrier protein]
3-oxo-hexadecenoyl-[acyl-carrier protein] + CO2 + CoA
show the reaction diagram
-
-
-
-
?
cis-9-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxo-cis-11-octadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
cis-9-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxo-cis-11-octadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
r
cis-9-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxo-cis-11-octadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
poor substrate, synthase I
-
-
?
cis-9-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxo-cis-11-octadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
poor substrate, synthase I
-
-
r
cis-9-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxo-cis-11-octadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
best substrate, synthase II
-
-
?
cis-9-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxo-cis-11-octadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
best substrate, synthase II
-
-
r
cis-mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
cis-mono-unsaturated 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
cis-mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
cis-mono-unsaturated 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
cis-mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
cis-mono-unsaturated 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
not cis-vaccenoyl-[acyl-carrier-protein], i.e. cis-11-octenoyl-[acyl-carrier-protein]
-
-
?
cis-mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
cis-mono-unsaturated 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
not cis-vaccenoyl-[acyl-carrier-protein], i.e. cis-11-octenoyl-[acyl-carrier-protein]
-
-
r
cis-mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
cis-mono-unsaturated 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
from C-10 to C-16, e.g. cis-5-decenoyl-[acyl-carrier-protein], cis-5-dodecenoyl-[acyl-carrier-protein], cis-7-tetradecenoyl-[acyl-carrier-protein], cis-9-hexenoyl-[acyl-carrier-protein], i.e. palmitoleoyl-[acyl-carrier-protein], poor substrates
-
-
-
cis-mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
cis-mono-unsaturated 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
from C-10 to C-16, e.g. cis-5-decenoyl-[acyl-carrier-protein], cis-5-dodecenoyl-[acyl-carrier-protein], cis-7-tetradecenoyl-[acyl-carrier-protein], cis-9-hexenoyl-[acyl-carrier-protein], i.e. palmitoleoyl-[acyl-carrier-protein], poor substrates
-
-
?
cis-mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
cis-mono-unsaturated 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
from C-10 to C-16, e.g. cis-5-decenoyl-[acyl-carrier-protein], cis-5-dodecenoyl-[acyl-carrier-protein], cis-7-tetradecenoyl-[acyl-carrier-protein], cis-9-hexenoyl-[acyl-carrier-protein], i.e. palmitoleoyl-[acyl-carrier-protein], poor substrates
-
-
r
decanoyl-ACP + malonyl-ACP
?
show the reaction diagram
P52410
-
-
-
?
decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
dodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
dodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
dodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
dodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
dodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
synthase I
-
-
?
decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
dodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
synthase IV
-
-
?
dodecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxotetradecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
dodecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxotetradecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
dodecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxotetradecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
dodecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxotetradecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
eicosanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxodocosanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
eicosanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxodocosanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-, P63454
Kas A and KasB
-
-
?
hexadecanoyl-CoA + malonyl-[acyl-carrier protein]
3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + CoA
show the reaction diagram
-, P63454
Kas A and KasB, preference for
-
-
?
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
not
-
-
-
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
poor substrate
-
-
?
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-, P63454
KasA and KasB, preference for
-
-
?
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
only synthase II, best substrate
-
-
?
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
i.e. palmitoyl-[acyl-carrier-protein]
-
-
?
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
i.e. palmitoyl-[acyl-carrier-protein]
-
-
?
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
i.e. palmitoyl-[acyl-carrier-protein]
-
-
?
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
preferred substrate, synthase III
-
-
?
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
ir
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
r
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
characterization of hexanoyl-enzyme intermediate
-
-
r
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
best substrate, synthase I
-
-
?
isobutyryl-CoA + malonyl-[acyl-carrier protein]
?
show the reaction diagram
-
synthase III
-
-
?
isobutyryl-CoA + malonyl-[acyl-carrier protein]
?
show the reaction diagram
P99159
synthase III
-
-
?
isobutyryl-CoA + malonyl-[acyl-carrier protein]
?
show the reaction diagram
-
isobutyryl-CoA is a precursor of branched-chain fatty acid synthesis
-
-
?
malonyl-ACP + lauroyl-ACP
?
show the reaction diagram
-
-
-
-
?
malonyl-ACP + lauroyl-CoA
?
show the reaction diagram
-
-
-
-
?
malonyl-ACP + palmitoyl-ACP
?
show the reaction diagram
-
-
-
-
?
malonyl-ACP + palmitoyl-CoA
?
show the reaction diagram
-
-
-
-
?
Malonyl-CoA
Acetyl-CoA + CO2
show the reaction diagram
-
decarboxylation reaction
-
-
?
malonyl-CoA + lauroyl-ACP
?
show the reaction diagram
-
-
-
-
?
malonyl-CoA + palmitoyl-ACP
?
show the reaction diagram
-
-
-
-
?
malonyl-phosphopantetheine-14-mer + lauroyl-ACP
?
show the reaction diagram
-
-
-
-
?
malonyl-phosphopantetheine-14-mer + palmitoyl-ACP
?
show the reaction diagram
-
-
-
-
?
malonyl-phosphopantetheine-16-mer + lauroyl-ACP
?
show the reaction diagram
-
-
-
-
?
malonyl-phosphopantetheine-16-mer + palmitoyl-ACP
?
show the reaction diagram
-
-
-
-
?
malonyl-phosphopantetheine-8-mer + lauroyl-ACP
?
show the reaction diagram
-
-
-
-
?
malonyl-phosphopantetheine-8-mer + palmitoyl-ACP
?
show the reaction diagram
-
-
-
-
?
malonyl-[acyl-carrier protein]
acetyl-[acyl-carrier protein] + CO2
show the reaction diagram
-
-
-
-
?
malonyl-[acyl-carrier protein]
acetyl-[acyl-carrier protein] + CO2
show the reaction diagram
-
catalyzes malonyl-[acyl-carrier-protein] decarboxylation independent of fatty acyl-[acyl-carrier-protein]
-
?
malonyl-[acyl-carrier protein]
acetyl-[acyl-carrier protein] + CO2
show the reaction diagram
-
decarboxylation with 2-3% the rate of beta-ketoacyl-[acyl-carrier-protein]-synthesis
-
?
malonyl-[acyl-carrier protein]
acetyl-[acyl-carrier protein] + CO2
show the reaction diagram
-
wild-type synthase I and mutants C163A and C163S
-
?
malonyl-[acyl-carrier protein] + lauroyl-CoA
? + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
malonyl-[acyl-carrier protein] + myristoyl-CoA
? + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
myristoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
myristoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
octadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoeicosanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
octadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoeicosanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
synthase I and II
-
-
?
octanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
ir
octanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
r
octanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
octanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
octanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
palmitoyl-[acyl-carrier protein] + malonyl-CoA
3-oxostearoyl-[acyl-carrier protein] + CO2 + CoA
show the reaction diagram
-
-
-
-
?
palmitoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
palmitoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
propionyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
beta-ketopentanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
ir
propionyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
beta-ketopentanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
absolutely specific for
-
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
r
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
r
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
r
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
r
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
r
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
P99159
-
-
ir
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
synthase II, parenchyma and lamina epidermis: palmitoyl-[acyl-carrier-protein] is the only product, sheath epidermis: acyl-[acyl-carrier-protein] up to C22 chain length
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
substrate specificity, overview
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
substrate specificity, overview
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
substrate specificity, overview
-
-
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
substrate specificity, overview
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
substrate specificity, overview
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
substrate specificity, overview
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
substrate specificity, overview
-
ir
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
substrate specificity, overview
-
r
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
substrate specificity, overview
-
r
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
substrate specificity, overview
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
substrate specificity, overview
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-, P63454
substrate specificity, overview
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
substrate specificity, overview
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
substrate specificity, overview
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
substrate specificity, overview
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
P0A3C5
substrate specificity, overview
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
preference for medium-chain length [acyl-carrier-protein]-substrates, synthase IV
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
synthase III, broad specificity
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
synthase III, broad specificity
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
not hexadecanoyl-[acyl-carrier-protein]
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
not hexadecanoyl-[acyl-carrier-protein]
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
specific for acyl-[acyl-carrier-protein] thioesters, not acyl-CoA or acyl pantetheine thioesters
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
specific for acyl-[acyl-carrier-protein] thioesters, not acyl-CoA or acyl pantetheine thioesters
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
fatty acid composition of Brassica napus wild-type and transgenic plants overexpressing the E. coli synthase III
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-, P63454
KasA and KasB, preference for long-chain acyl-[acyl-carrier-protein]s with at least 16 C-atoms
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
not octodecanoyl-[acyl-carrier-protein], i.e stearoyl-[acyl-carrier-protein]
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
not octodecanoyl-[acyl-carrier-protein], i.e stearoyl-[acyl-carrier-protein]
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
Euglena gracilis Z
-
-
-
?
stearoyl-[acyl-carrier protein] + malonyl-CoA
3-oxoeicosanoyl-[acyl-carrier protein] + CO2 + CoA
show the reaction diagram
-
only in sheath epidermis
-
-
?
tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
-
tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
poor substrate
-
-
r
tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
synthase I
-
-
?
tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
i.e. myristoyl-[acyl-carrier-protein], only synthase II
-
-
?
malonyl-[acyl-carrier protein] + palmitoyl-CoA
? + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
-
enzyme also catalyzes a malonyl-CoA-CO2 exchange reaction, overview: substrates and kinetics
-
-
-
additional information
?
-
P0A3C5
synthase III, FabH, has a preference for straight short-chain CoA primers
-
-
-
additional information
?
-
-
synthase I and II have nearly the same substrate specificity
-
-
-
additional information
?
-
-
synthase I is specific for short-chain fatty acid prolongation while synthase II is active in C18 formation from C16
-
-
-
additional information
?
-
-
synthase III isozymes also utilize iso- and anteiso-branched-chain acyl-CoA primers as substrates
-
-
-
additional information
?
-
-
malonyl-CoA-CO2 exchange reaction
-
-
-
additional information
?
-
-
important role in generation of the lipoic acid precursor octanoyl-acyl-carrier protein
-
-
-
additional information
?
-
-
enzyme is able to use Escherichia coli acyl-carrier protein. Enzyme extends longer, physiologically relevant acyl-CoA primers when paired with its native acyl-carrier protein than with Escherichia coli acyl-carrier protein
-
-
-
additional information
?
-
Q8L3X9
no substrate: acetyl-[acyl-carrier protein]
-
-
-
additional information
?
-
-
preference for acyl-[acyl-carrier protein] in decreasing order: C12, C10, C6, C8, C4, C2, C14, C16
-
-
-
additional information
?
-
-
rank of activity in decreasing order: isobutyryl-CoA, hexanoyl-CoA, butyryl-CoA, isovaleryl-CoA, acetyl-CoA
-
-
-
additional information
?
-
-
inhibition of FabH leads to loss of accumulation of acetoacetyl-ACP
-
-
-
additional information
?
-
Q586W9, -
the organism, with the unusual lipid metabolism of the trypanosome, uses a microsomal elongase pathway, ELO, for de novo fatty acid synthesis, a second FAS pathway with a type II synthase is localized to the mitochondrion and is essential for bloodstream and procyclic life cycle stages of the parasite, in vivo precursors of mitochondrial fatty acid synthesis, overview
-
-
-
additional information
?
-
-
enzyme active site structure of wild-type and mutants with or without bound C12 or C8, overview
-
-
-
additional information
?
-
-
FabH produces C14 long-chain beta-ketoacyl-ACP
-
-
-
additional information
?
-
-, Q9NWU1
KAS catalyzes the C2 fatty acid elongation reaction using a Cys-His-His catalytic triad
-
-
-
additional information
?
-
Q586W9, -
octanoate, the precursor for lipoic acid synthesis, is also produced by the mitochondrial FAS
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxo-6-octadecenoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
reaction is part of petroselinic acid biosynthesis, synthase IV
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
part of non-associated fatty acid synthase system of plants and prokaryotes
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
part of non-associated fatty acid synthase system of plants and prokaryotes
-
-
ir
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
part of non-associated fatty acid synthase system of plants and prokaryotes
-
-
r
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
part of non-associated fatty acid synthase system of plants and prokaryotes
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
part of non-associated fatty acid synthase system of plants and prokaryotes
-
-
-
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
involved in biosynthesis of saturated fatty acids
-
-
ir
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
involved in biosynthesis of saturated fatty acids
-
-
r
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
involved in biosynthesis of saturated fatty acids
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
involved in biosynthesis of saturated fatty acids
-
-
r
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
key enzyme for regulation of medium-chain fatty acid synthesis in seeds
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
P99159
initiation of fatty acid biosynthesis, part of fatty acid synthase II
-
-
ir
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
involved in biosynthesis of unsaturated and saturated fatty acids
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-, P63454
part of fatty acid synthase system I and II, involved in cell wall mycolic acids biosynthesis, overview
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
initiation of fatty acid biosynthesis with acetyl-CoA as a primer, overview substrate specificity
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
responsible for chain elongation during de novo fatty acid synthesis
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
responsible for chain elongation during de novo fatty acid synthesis
-
-
-
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
involved in biosynthesis of mono-unsaturated long-chain fatty acids
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
initiation of both straight- and branched-chain fatty acid biosynthesis resulting in a wide variety of produced fatty acids in vivo
-
-
?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
acyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
an acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
a 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
show the reaction diagram
-
the endoplasmic reticulum enzyme is involved in synthesis of very long chain fatty acids
-
-
?
cis-3-decenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
3-oxo-cis-5-dodecenoyl-[acyl-carrier protein] + acyl-carrier protein
show the reaction diagram
-
key step in the unsaturated fatty acid, UFA, synthesis pathway. FabB is negatively regulated by the FabR protein
the product is not incorporated into the complex lipids of the bacterium
-
?
additional information
?
-
-
important role in generation of the lipoic acid precursor octanoyl-acyl-carrier protein
-
-
-
additional information
?
-
-
inhibition of FabH leads to loss of accumulation of acetoacetyl-ACP
-
-
-
additional information
?
-
Q586W9, -
the organism, with the unusual lipid metabolism of the trypanosome, uses a microsomal elongase pathway, ELO, for de novo fatty acid synthesis, a second FAS pathway with a type II synthase is localized to the mitochondrion and is essential for bloodstream and procyclic life cycle stages of the parasite, in vivo precursors of mitochondrial fatty acid synthesis, overview
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
4-phenyl-5-phenylimino-[1,2,4]dithiazolidin-3-one
P99159
i.e. HR19; strong, synthase III
5-chloro-4-phenyl-[1,2]-dithiol-3-one
P99159
complete inhibition at 0.01 nM, synthase III; i.e. HR12
acyl-CoA
-
at high concentrations
arsenite
-
weak at 1 mM
arsenite
-
synthase II and I
arsenite
-
synthase III
Butyryl-CoA
-
synthase III mutants C122Aand C122Q: inhibition of decarboxylation activity in presence of
C75
-
a synthetic inhibitor of fatty acid synthase, inhibits also the beta-ketoacyl synthase, not in simple first order rate reaction, C75 is a competitive, irreversible inhibitor of the overall reaction, the beta-ketoacyl synthase activity and the enoyl reductase activity substrates do not protect the thioesterase activity of rat liver FAS from inactivation by C75, but malonyl-CoA and acetyl-CoA protect the FAS beta-ketoacyl synthase reaction from inactivation by C75 in a competitive manner, overview
cerulenin
-
antibiotic, protection by hexanoyl-[acyl-carrier-protein], decanoyl-[acyl-carrier-protein], and tetradecanoyl-[acyl-carrier-protein]
cerulenin
-
no inhibition
cerulenin
-
synthase II, moderate
cerulenin
-
12-carbon mycotoxin, irreversible inhibition, binding motif; at 0.05 mM: wild-type is completely inactivated, mutant I108F and A193M show only 10% reduced activity
cerulenin
-
analysis of the binding mechanism, mimicks the condensation transition state; FabB: inhibition is irreversible
cerulenin
-, P63454
Kas A and KasB
cerulenin
P99159
weak, synthase III
cerulenin
-
synthase IV
cerulenin
-
strong
cerulenin
-
50% inhibition at 0.2 mM
cerulenin
-
specific inhibition of the enzyme
cerulenin
-
irreversible inhibition, IC50: 0.0158 mM
cerulenin
-, Q9NWU1
-
iodoacetamide
-
no inhibition of malonyl-[acyl-carrier-protein]-decarboxylation
iodoacetamide
-
-
iodoacetamide
-
after incubation with 2-mercaptethanol
iodoacetamide
-
acyl-[acyl-carrier-protein]-derivatives, e.g. acetyl-[acyl-carrier-protein], hexanoyl-CoA, octanoyl-CoA, decanoyl-CoA protect
iodoacetamide
-
acyl-[acyl-carrier-protein]-derivatives, e.g. acetyl-[acyl-carrier-protein], hexanoyl-CoA, octanoyl-CoA, decanoyl-CoA protect; synthase I and II
iodoacetamide
-
kinetics
N-(3-pyridinyl)-hexanamide
-
result of an in silicio screening, antimicrobial activity, binding to beta-ketoacyl-[acyl-carrier-protein] synthase I characterized by saturation-transfer difference NMR spectroscopy
-
N-ethylmaleimide
-
no inhibition of malonyl-[acyl-carrier-protein]-decarboxylation
N-ethylmaleimide
-
-
N-ethylmaleimide
-
acyl-[acyl-carrier-protein]-derivatives protect
N-ethylmaleimide
-
after incubation with 2-mercaptoethanol
N-ethylmaleimide
-
5 mM
N-ethylmaleimide
-
-
p-chloromercuribenzoate
-
complete inhibition at 1 mM
palmitoyl-CoA
-
substrate inhibition above 0.003 mM
platencin
-
exhibits a broad-spectrum Gram-positive antibacterial activity through inhibition of fatty acid biosynthesis, targets the two essential proteins, beta-ketoacyl-[acyl carrier protein] synthase II and III, i.e. FabF and FabH, purified FabH IC50: 0.016 mM, overview
SB418011
P0A3C5
strong, synthase III
thiolactomycin
-
synthase III
thiolactomycin
-
synthase III
thiolactomycin
P0A3C5
synthase III; weak
thiolactomycin
-
analysis of the binding mechanism, mimics malonyl-[acyl-carrier-protein]; FabB: inhibition is reversible, competitive
thiolactomycin
-, P63454
Kas A and KasB
thiolactomycin
P99159
synthase III; weak
thiolactomycin
-
competitive inhibition, IC50: 0.0236 mM
thiolactomycin
-
-
Urea
-
about 50% inhibition at 1 M, complete inactivation at 4 M
[Acyl-carrier-protein]
-
wild-type synthase I and mutants, not mutants C163S and K328A, overview
isobutyryl-CoA
-
synthase III mutants C122Aand C122Q: inhibition of decarboxylation activity in presence of
additional information
-
the enzyme shows strong substrate inhibition
-
additional information
-
in vivo inhibition assays, no inhibition by platensimycin
-
additional information
-, Q9NWU1
inhibitor binding structure determination
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
activation
2-mercaptoethanol
-
required in high concentration, 0.5 M
2-mercaptoethanol
-
required
dithiothreitol
-
activation
dithiothreitol
-
activation; can replace 2-mercaptoethanol
dithiothreitol
-
activation; required
dithiothreitol
-
required
EDTA
-
7 mM, stimulates
phosphate
-
activation, 0.2 M
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0024
-
acetyl-CoA
-
synthase III
0.0062
-
acetyl-CoA
P99159
synthase III
0.0403
-
acetyl-CoA
P0A3C5
synthase III
0.019
-
acetyl-[acyl-carrier protein]
-
pH 7.0
0.031
-
acetyl-[acyl-carrier protein]
-
synthase I
0.04
-
acetyl-[acyl-carrier protein]
-
acyl-carrier-protein, fatty acyl transfer between tetradecanoyl-CoA and acyl-carrier-protein
0.04
-
acetyl-[acyl-carrier protein]
-
synthase II
0.05
-
acetyl-[acyl-carrier protein]
-
pH 6.8, 37C
0.0039
-
butanoyl-[acyl-carrier protein]
-
pH 6.8, 37C
0.00071
-
Butyryl-CoA
-
synthase III
0.0023
-
Butyryl-CoA
P99159
synthase III
0.403
-
C10-[acyl-carrier protein]
-
pH 7.5, 37C, recombinant enzyme
0.196
-
C12-[acyl-carrier protein]
-
pH 7.5, 37C, recombinant enzyme
0.437
-
C14-[acyl-carrier protein]
-
pH 7.5, 37C, recombinant enzyme
0.211
-
C4-[acyl-carrier protein]
-
pH 7.5, 37C, recombinant enzyme
0.116
-
C6-[acyl-carrier protein]
-
pH 7.5, 37C, recombinant enzyme
0.198
-
C8-[acyl-carrier protein]
-
pH 7.5, 37C, recombinant enzyme
0.012
-
cis-3-decenoyl-[acyl-carrier protein]
-
synthase I
0.014
-
cis-3-decenoyl-[acyl-carrier protein]
-
synthase II
0.017
-
cis-9-hexadecenoyl-[acyl-carrier protein]
-
synthase II
0.138
-
cis-9-hexadecenoyl-[acyl-carrier protein]
-
synthase I
0.0018
-
decanoyl-[acyl-carrier protein]
-
pH 6.8, 37C
0.0031
-
decanoyl-[acyl-carrier protein]
-
synthase IV
0.0133
-
decanoyl-[acyl-carrier protein]
-
synthase II
0.0023
-
dodecanoyl-[acyl-carrier protein]
-
pH 7.0, 37C
0.0095
-
dodecanoyl-[acyl-carrier protein]
-
pH 6.8, 37C
0.002
-
eicosanoyl-[acyl-carrier protein]
-, P63454
isozyme Kas B
0.0025
-
eicosanoyl-[acyl-carrier protein]
-, P63454
isozyme Kas A
0.0019
-
hexanoyl-[acyl-carrier protein]
-
pH 6.8, 37C
0.0075
-
hexanoyl-[acyl-carrier protein]
-
-
0.00032
-
isobutyryl-CoA
P99159
synthase III
0.00041
-
isobutyryl-CoA
-
synthase III
0.0032
-
lauroyl-ACP
-
wild type protein, acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 6.5, 25C
0.0039
-
lauroyl-ACP
-
wild type protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25C
0.0067
-
lauroyl-ACP
-
K151Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25C
0.05
-
lauroyl-ACP
-
R62Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25C
0.056
-
lauroyl-ACP
-
R66Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25C
0.06
-
lauroyl-ACP
-
K63Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25C
0.0586
-
Lauroyl-CoA
-
wild type protein, acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 6.5, 25C
0.0058
-
malonyl-ACP
-
donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25C
0.0115
-
malonyl-ACP
-
wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25C
0.004
-
malonyl-CoA
-
in presence of [acyl-carrier-protein]
0.0055
-
malonyl-CoA
-
-
0.009
-
malonyl-CoA
-
donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25C
0.016
-
malonyl-CoA
-
without [acyl-carrier-protein]
0.153
-
malonyl-CoA
-
wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25C
0.0086
-
malonyl-phosphopantetheine-14-mer
-
donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25C
-
0.0158
-
malonyl-phosphopantetheine-14-mer
-
wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25C
-
0.0051
-
malonyl-phosphopantetheine-16-mer
-
donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25C
-
0.029
-
malonyl-phosphopantetheine-16-mer
-
wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25C
-
0.0047
-
malonyl-phosphopantetheine-8-mer
-
donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25C
-
0.0142
-
malonyl-phosphopantetheine-8-mer
-
wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25C
-
0.0037
-
malonyl-[acyl-carrier protein]
-
synthase III
0.0135
-
malonyl-[acyl-carrier protein]
-, P63454
isozymes Kas A and KasB; isozymes Kas A and KasB
0.0186
-
malonyl-[acyl-carrier protein]
P0A3C5
synthase III
0.0508
-
myristoyl-[acyl-carrier protein]
-
pH 6.8, 37C
0.0109
-
octanoyl-[acyl-carrier protein]
-
pH 6.8, 37C
0.0004
-
palmitoyl-CoA
-
acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 8.5, 25C
0.0014
-
palmitoyl-[acyl-carrier protein]
-, P63454
isozyme Kas B
0.0032
-
palmitoyl-[acyl-carrier protein]
-, P63454
isozyme Kas A
0.0036
-
palmitoyl-[acyl-carrier protein]
-
synthase II
0.0091
-
tetradecanoyl-[acyl-carrier protein]
-
-
0.0139
-
tetradecanoyl-[acyl-carrier protein]
-
synthase II
0.025
-
malonyl-[acyl-carrier protein]
-
pH 7.0
additional information
-
additional information
-
-
-
additional information
-
additional information
-
kinetic data of synthase I and II at different temperatures with various substrates
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
mutants
-
additional information
-
additional information
-
kinetics
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
91.2
-
butanoyl-[acyl-carrier protein]
-
pH 6.8, 37C
1.32
-
butyryl-[acyl-carrier protein]
-
pH 7.5, 37C, recombinant enzyme
3.07
-
C10-[acyl-carrier protein]
-
pH 7.5, 37C, recombinant enzyme
0.69
-
C12-[acyl-carrier protein]
-
pH 7.5, 37C, recombinant enzyme
1.28
-
C14-[acyl-carrier protein]
-
pH 7.5, 37C, recombinant enzyme
1.32
-
C4-[acyl-carrier protein]
-
pH 7.5, 37C, recombinant enzyme
0.96
-
C6-[acyl-carrier protein]
-
pH 7.5, 37C, recombinant enzyme
1.63
-
C8-[acyl-carrier protein]
-
pH 7.5, 37C, recombinant enzyme
3.07
-
decanoyl-[acyl-carrier protein]
-
pH 7.5, 37C, recombinant enzyme
551
-
decanoyl-[acyl-carrier protein]
-
pH 6.8, 37C
0.69
-
dodecanoyl-[acyl-carrier protein]
-
pH 7.5, 37C, recombinant enzyme
303
-
dodecanoyl-[acyl-carrier protein]
-
pH 6.8, 37C
0.01
-
eicosanoyl-[acyl-carrier protein]
-, P63454
isozyme KasB
0.075
-
eicosanoyl-[acyl-carrier protein]
-, P63454
isozyme KasA
0.96
-
hexanoyl-[acyl-carrier protein]
-
pH 7.5, 37C, recombinant enzyme
353
-
hexanoyl-[acyl-carrier protein]
-
pH 6.8, 37C
0.025
-
lauroyl-ACP
-
K151Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25C
0.033
-
lauroyl-ACP
-
wild type protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25C
0.053
-
lauroyl-ACP
-
R66Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25C
0.057
-
lauroyl-ACP
-
wild type protein, acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 6.5, 25C
0.077
-
lauroyl-ACP
-
R62Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25C
0.083
-
lauroyl-ACP
-
K63Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25C
0.073
-
Lauroyl-CoA
-
wild type protein, acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 6.5, 25C
0.011
-
malonyl-ACP
-
wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25C
0.47
-
malonyl-ACP
-
donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25C
0.12
-
malonyl-CoA
-
wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25C
0.35
-
malonyl-CoA
-
donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25C
0.04
-
malonyl-phosphopantetheine-14-mer
-
wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25C
-
0.38
-
malonyl-phosphopantetheine-14-mer
-
donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25C
-
0.11
-
malonyl-phosphopantetheine-16-mer
-
wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25C
-
0.3
-
malonyl-phosphopantetheine-16-mer
-
donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25C
-
0.018
-
malonyl-phosphopantetheine-8-mer
-
wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25C
-
0.35
-
malonyl-phosphopantetheine-8-mer
-
donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25C
-
0.0233
-
malonyl-[acyl-carrier protein]
-, P63454
isozyme KasB
0.08
-
malonyl-[acyl-carrier protein]
-, P63454
isozyme KasA
6
-
myristoyl-[acyl-carrier protein]
-
pH 6.8, 37C
1.63
-
octanoyl-[acyl-carrier protein]
-
pH 7.5, 37C, recombinant enzyme
66
-
octanoyl-[acyl-carrier protein]
-
pH 6.8, 37C
0.08
-
palmitoyl-CoA
-
acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 8.5, 25C
0.0267
-
palmitoyl-[acyl-carrier protein]
-, P63454
isozyme KasB
0.0883
-
palmitoyl-[acyl-carrier protein]
-, P63454
isozyme KasA
1.28
-
tetradecanoyl-[acyl-carrier protein]
-
pH 7.5, 37C, recombinant enzyme
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.83
-
lauroyl-ACP
-
R66Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25C
226438
1.2
-
lauroyl-ACP
-
K63Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25C
226438
1.5
-
lauroyl-ACP
-
R62Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25C
226438
3.7
-
lauroyl-ACP
-
K151Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25C
226438
8.3
-
lauroyl-ACP
-
wild type protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25C
226438
18.3
-
lauroyl-ACP
-
wild type protein, acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 6.5, 25C
226438
1.27
-
Lauroyl-CoA
-
wild type protein, acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 6.5, 25C
12519
10
-
malonyl-ACP
-
wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25C
21863
86.7
-
malonyl-ACP
-
donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25C
21863
0.78
-
malonyl-CoA
-
wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25C
12844
38.3
-
malonyl-CoA
-
donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25C
12844
3
-
malonyl-phosphopantetheine-14-mer
-
wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25C
0
48.3
-
malonyl-phosphopantetheine-14-mer
-
donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25C
0
3.8
-
malonyl-phosphopantetheine-16-mer
-
wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25C
0
60
-
malonyl-phosphopantetheine-16-mer
-
donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25C
0
1.3
-
malonyl-phosphopantetheine-8-mer
-
wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25C
0
75
-
malonyl-phosphopantetheine-8-mer
-
donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25C
0
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.01
-
thiolactomycin
-
pH 7.5, 37C, recombinant enzyme
16
-
C75
-
pH 7.0, 22C, inactivation of the whole reaction of FAS
additional information
-
additional information
-
inhibition kinetics
-
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0158
-
cerulenin
-
irreversible inhibition, IC50: 0.0158 mM
0.016
-
platencin
-
exhibits a broad-spectrum Gram-positive antibacterial activity through inhibition of fatty acid biosynthesis, targets the two essential proteins, beta-ketoacyl-[acyl carrier protein] synthase II and III, i.e. FabF and FabH, purified FabH IC50: 0.016 mM, o
0.0236
-
thiolactomycin
-
competitive inhibition, IC50: 0.0236 mM
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.0000034
-
-, P63454
substrate palmitoyl-CoA
0.000006
-
-, P63454
substrate palmitoyl-CoA
0.000032
-
-, P63454
substrate palmitoyl-[acyl-carrier-protein]
0.000088
-
-, P63454
substrate palmitoyl-[acyl-carrier-protein]
0.0017
-
-
substrate hexanoyl-[acyl-carrier protein], pH 7.0, 37C
0.005
-
P0A3C5
synthase III
0.0055
-
-
substrate octanoyl-[acyl-carrier protein], pH 7.0, 37C
0.0062
-
-
substrate decanoyl-[acyl-carrier protein], pH 7.0, 37C
0.0072
-
-
substrate dodecanoyl-[acyl-carrier protein], pH 7.0, 37C
0.0083
-
-
purified enzyme
0.009
-
-
substrate eicosanoyl-[acyl-carrier-protein], pH 7.0, 37C
0.0093
-
-
substrate palmitoyl-[acyl-carrier protein], pH 7.0, 37C
0.0095
-
-
substrate myristoyl-[acyl-carrier protein], pH 7.0, 37C
0.0104
-
-
substrate octadecanoyl-[acyl-carrier-protein], pH 7.0, 37C
0.105
-
-
purified recombinant enzyme with substrate C6-[acyl-carrier-protein]
0.403
-
-
purified enzyme
0.8
-
-
purified synthase I
3.81
-
-
purified enzyme
5.5
-
-
synthase I
6.3
-
-
synthase II
7
-
-
purified native enzyme
7.5
-
-
purified synthase II
8.04
-
-
partially purified enzyme
9
-
-
purified synthase I
additional information
-
-
fatty acid synthase activity, malonyl-CoA incorporation
additional information
-
-
-
additional information
-
-
-
additional information
-
-
-
additional information
-
-
comparison of activity, recombinant and native enzyme
additional information
-
-
wild-type and transgenic Brassica napus plants, expressing the E. coli synthase III
additional information
-
-
-
additional information
-
-
mutants
additional information
-
Q8L3X9
enzyme from fresh extract synthesizes prominent amounts of C14 and C16 acyl chains. After storage at -20C for one or several weeks, enzyme synthesizes predominantly C8 acyl chains
additional information
-
-
transfer of [3H]myristate from ACP to wild-type and Lys328 mutant KAS I
additional information
-
-
-
additional information
-
Q586W9, -
-
additional information
-
-
development of an elongation assay with FabF, EC 2.3.1.179, and FabH
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
6.1
-
synthase II
6.6
7.7
-
synthase III
6.8
-
-, P63454
isozyme KasA, HEPES buffer
6.8
-
-
assay at
6.8
-
-
decarboxylation assay at, inactive at pH 4.0-5.0
7
-
-
assay at
7
-
-, P63454
isozyme KasB, phosphate buffer
7
-
-
assay at
7
-
-
assay at
7.2
-
-
synthase I
7.2
-
P99159
synthase III
7.5
-
-
assay at
7.5
-
-
assay at
7.5
-
Q586W9, -
assay at
8
-
-
assay at
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.2
7.6
-
synthase II, about half-maximal activity at pH 5.2 and pH 7.6
5.6
7.2
-
synthase II, about half-maximal activity at pH 5.6 and pH 7.2
5.8
8
P99159
synthase III
5.9
6.8
-
synthase I, about half-maximal activity at pH 5.9 and pH 6.8
6.3
8
-
synthase I, about half-maximal activity at pH 6.3 and pH 8.0
6.5
8
-
sharp drop in activity below pH 6.5 and above pH 8.0
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
-
-
assay at
22
-
-
assay at room temperature
22
-
-
assay at room temperature
25
-
-
assay at
25
-
-
assay at
27
-
-
assay at
30
-
-
assay at
30
-
-
assay at
30
-
-
assay at
35
-
-
assay at
37
-
-
assay at
37
-
-
assay at
37
-
-
assay at
37
-
-
assay at
37
-
Q586W9, -
assay at
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
42
-, P63454
30-50% activity at 30 and 42C; 30-50% activity at 30 and 42C
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
low level
Manually annotated by BRENDA team
-
sheath and lamina, and underlying parenchyma
Manually annotated by BRENDA team
-
most abundant
Manually annotated by BRENDA team
-
low level
Manually annotated by BRENDA team
P52410
also found in 7-d-old seedling, root, flower, young silique, embryos at late cotyledon stage and stalk
Manually annotated by BRENDA team
-
germinating
Manually annotated by BRENDA team
-
germinating
Manually annotated by BRENDA team
-
germinating; specific isoform
Manually annotated by BRENDA team
-
low level
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Euglena gracilis Z
-
-
-
Manually annotated by BRENDA team
Q586W9, -
enzyme in the fatty acid synthase complex
-
Manually annotated by BRENDA team
Q586W9, -
enzyme in the elongase complex
Manually annotated by BRENDA team
-, Q9NWU1
beta-ketoacyl [ACP] synthase from the mitochondrial type II fatty acid synthase
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Brucella abortus (strain 2308)
Brucella abortus (strain 2308)
Brucella abortus (strain 2308)
Brucella abortus (strain 2308)
Brucella abortus (strain 2308)
Burkholderia xenovorans (strain LB400)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Moorea producens 3L
Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Xanthomonas campestris pv. campestris (strain ATCC 33913 / NCPPB 528 / LMG 568)
Xanthomonas oryzae pv. oryzae (strain KACC10331 / KXO85)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
38000
-
-
synthase III-like polyketid synthase, gel filtration, amino acid sequence determination
56000
-
-
synthase I, gel filtration
57500
-
-
synthase II, gel filtration
60000
-
P0A3C5
synthase III, gel filtration
63000
-
-
synthase III, gel filtration
66000
-
-
equilibrium centrifugation
66000
-
-
synthase I, gel filtration
67600
-
-
gel filtration
67600
-
P99159
gel filtration
70000
-
-
about, gel filtration
72000
-
-
recombinant enzyme, gel filtration
76000
-
-
synthase II, gel filtration
80000
-
-
synthase I, sedimentation equilibrium method
85000
-
-
synthase II, sedimentation equilibrium method
86700
-
-
synthase I, gel filtration
87400
-
-
synthase II, gel filtration
87800
-
-
synthase II, gel filtration
90000
-
-
gel filtration
118000
-
-
gel filtration
additional information
-
-
amino acid composition, synthase I and II
additional information
-
-
amino acid sequence alignment
additional information
-
-
amino acid sequence alignment
additional information
-
P0A3C5
amino acid sequence alignment
additional information
-
-
synthase I mutants
additional information
-
P99159
amino acid sequence alignment
additional information
-
-
amino acid sequence alignment
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 39800, FabH2, SDS-PAGE; x * 42700, FabH1, SDS-PAGE
?
Q8L3X9
x * 47000, SDS-PAGE
?
-
x * 45800, SDS-PAGE, recombinant protein without N-terminal targeting sequence, with His-tag
?
-
x * 44000, His-tagged protein, SDS-PAGE
dimer
-
2 * 35000, SDS-PAGE; 2 * 37000, gel filtration after 6 M guanidinium chloride treatment
dimer
-
2 * 34000, SDS-PAGE
dimer
-
2 * 43000-44000, synthase I, SDS-PAGE; 2 * 44000-45000, synthase II, SDS-PAGE
dimer
-
2 * 43000, SDS-PAGE
dimer
-
2 * 45000, SDS-PAGE; 2 * 46000, alpha2, synthase I, SDS-PAGE
dimer
-
2 * 46000, SDS-PAGE
dimer
-
2 * 40500, synthase III, SDS-PAGE
dimer
-
2 * 37000, recombinant synthase III, SDS-PAGE
dimer
-
crystal structure analysis of subunits
dimer
P99159
2 * 37000, recombinant synthase III, SDS-PAGE
dimer
Q8L3X9
crystallization data
dimer
-, Q9NWU1
secondary structure, a dimer with an alpha-beta-alpha-beta-alpha-thiolase fold capped by an alpha-helical region connecting the strands of the N-terminal beta-sheet, crystal structure analysis
monomer
-
1 * 38000, synthase III-like polyketid synthase, SDS-PAGE
additional information
-
vertebrates, yeast and Mycobacteria fatty acid synthetases contain all individual activities on one or two multifunctional polypeptide chains, plant, Escherichia coli and other prokaryotic fatty acid synthases are non-associated systems of individual enzymes
additional information
-
vertebrates, yeast and Mycobacteria fatty acid synthetases contain all individual activities on one or two multifunctional polypeptide chains, plant, Escherichia coli and other prokaryotic fatty acid synthases are non-associated systems of individual enzymes
additional information
-
synthase I mutants
additional information
P0A953
comparison of crystal structures of the active sites of Escherichia coli KAS I with human cytosolic acetoacetyl-CoA thiolase and bacterial thiolase, active sites with four highly conserved loop structures, overview
additional information
-
comparison of active sites in isoforms KAS I, KAS II and related enzymes
additional information
-
analysis of wild-type and mutant active sites with bound substrates at different pH, overview
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
proteolytic modification
Q8L3X9
20 amino acid mitochondrial targeting sequence
proteolytic modification
-
mitochondrial targeting sequence of 38 residues
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
comparison of crystal structures of the active sites of Escherichia coli KAS I with human cytosolic acetoacetyl-CoA thiolase and bacterial thiolase, overview
P0A953
purified recombinant wild-type and mutant enzymes, free or bound to C8 and C12, respectively, X-ray diffraction structure determination and analysis at 1.8-2.4 A resolution
-
structure analysis, three-dimensional model of enzyme complexed with inhibitors thiolactomycin and cerulenin
-
purified recombinant enzyme free or complexed with acyl-CoA, hanging drop vapour diffusion method, 0.002 ml of protein solution, containing 0.087 mg of protein with or without 4 mM hexanoyl-CoA, mixed with 0.002 ml of reservoir solution containing 24% w/v PEG 3350 and 0.2 M NH4Cl, 5-8 days at room temperature, rod-shaped single crystals, X-ray diffraction structure determination and analysis at 1.6 A resolution, structure modeling
-, Q9NWU1
in complex with lauroyl-CoA
-
hanging drop vapour diffusion method, protein solution: 25 mM Tris-HCl, pH 8.0, 300 mM NaCl, 75 mM imidazole, reservoir solution: 0.085 M tri-sodium citrate, 0.17 M ammonium acetate, 22% w/v polyethylene glycol 4000, 15% v/v glycerol as cryo-protectant, 15% w/v 1,2,3-heptanetriol, x-ray structure analysis, three-dimensional model
-
hanging-drop vapour-diffusion method, ammonium sulfate
-
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
43
-
-
t1/2 synthase I: 18 min, t1/2 synthase II: 81 min
100
-
-
inactivation after 1 min
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
dithiothreitol stabilizes
-
proteinase inhibitors stabilize during purification
-
Triton X-100 facilitates extraction from oil seeds
-
unstable during purification
-
2-mercaptoethanol stabilizes
-
EDTA stabilizes
-
unstable during purification
-
freezing and thawing inactivates
-
dithiothreitol stabilizes
-
glycerol stabilizes
-
Triton X-100 stabilizes
-
OXIDATION STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
oxidation by formic acid
-
486918
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
0C, unstable in crude extracts lacking glycerol. Enzyme from fresh extract synthesizes prominent amounts of C14 and C16 acyl chains. After storage at -20C for one or several weeks, enzyme synthesizes predominantly C8 acyl chains
Q8L3X9
-70 to -20C, 90% loss of activity within 7 days
-
-20C, 90% loss of activity within 1 year
-
-20C, at least 1 month
-
-20C, complete loss of activity in the presence of EDTA and 2-mercaptoethanol
-
-20C, several months
-
-20C, t1/2: 1 month
-
-70C, at least 1 month
-
-80C, several weeks in the presence of 20% v/v glycerol, 0.1% Triton X-100 and dithiothreitol
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
recombinant enzyme
Q8L3X9
recombinant His-tagged proteins FabH1 and FabH2 from E. coli
-
synthases I and II
-
recombinant His-tagged seed-specific isoform from E. coli
-
recombinant His-tagged synthase IV from E. coli
-
immobilized metal ion affinity chromatography
-
immobilized metal ion affinity chromatography (Ni2+)
-
isolation of hexanoyl-enzyme intermediate complex
-
recombinant synthase I and mutants as His-tagged protein from E. coli
-
recombinant synthases I, i.e. FabA, II, i.e. FabB, and III, i.e.FabH
-
synthases I and II
-
recombinant His-tagged protein from E. coli
-
native enzyme from kernel microsomes, solubilization and separation from KAS II by treatment with Triton X-100 and anion exchange chromatography
-
recombinant His-tagged enzyme from Escherichia coli strains XL-1 blue and M15
-, Q9NWU1
3 proteins alpha2, alpha,beta and beta2
-
immobilized metal ion affinity chromatography (Ni2+)
-
recombinant His-tagged protein from E. coli; recombinant His-tagged protein from E. coli
-, P63454
recombinant soluble NusA-fusion protein FabBF from Escherichia coli strain BL21(DE3) by and gel filtration
-
native fatty acid synthase from liver in several steps including gel filtration as the last step
-
synthase I
-
synthase I; synthases II
-
synthase III, native wild-type and recombinant from E. coli E103S
-
synthases II
-
recombinant His-tagged synthase III from E. coli
P99159
recombinant His-tagged synthase III from E. coli
P0A3C5
recombinant from E. coli as His-tagged protein
-
recombinant from E. coli as His-tagged protein; wild-type and mutants
-
synthase III-like polyketid synthase recombinant from Streptomyces lividans
-
synthase II, recombinant from E. coli
-
immobilized metal ion affinity chromatography (Ni2+), anion-exchange chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
complements KAS II defect in Escherichia coli
Q8L3X9
expression of His-tagged FabH1 and FabH2 in Escherichia coli INValphaF'
-
construction of a cDNA library from developing seeds, DNA sequence analysis, expression as His-tagged enzyme in Escherichia coli
-
expression of His-tagged synthase IV in Escherichia coli BL21 (DE3)
-
expression of synthase I and mutants as His-tagged proteins in Escherichia coli
-
His-tagged protein expressed in Escherichia coli BL21(DE3)
-
His-tagged protein expressed in Escherichia coli pLysS
-
synthase III, overexpression in Escherichia coli strain JM101, construction of binary expression vectors for transformation of Brassica napus
-
synthases I, II, and III, recombinant expression
-
expression as His-tagged protein in Escherichia coli
-
expression of the His-tagged enzyme in Escherichia coli strains XL-1 blue and M15
-, Q9NWU1
cDNA cloned into lambda2AB-II
-
expression of KasA and KasB as His-tagged protein in Escherichia coli; expression of KasA and KasB as His-tagged protein in Escherichia coli
-, P63454
His-tagged proteinsexpressed in Mycobacterium smegmatis mc2155
-
gene fabBF, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of the soluble NusA-fusion protein FabBF in Escherichia coli strain BL21(DE3)
-
synthase III, overexpression of His-tagged FabH in Escherichia coli BL21 (DE3)
P99159
overexpression of His-tagged FabH in Escherichia coli BL21 (DE3)
P0A3C5
overexpression of fabH in Escherichia coli as His-tagged protein, synthase III
-
plasmid-based expression of synthase III wild-type and mutants in Streptomyces glaucescens and His-tagged in Escherichia coli
-
expression of synthase III-like polyketid synthase in Streptomyces lividans
-
expression in Escherichia coli
-
expression of wild-type and mutant synthase II in Escherichia coli
-
His-tagged protein expressed in Escherichia coli BL21(DE3)
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
C163A
-
synthase I, site-directed mutagenesis, active site mutant, no activity, but decarboxylates malonyl-[acyl-carrier-protein]
C163S
-
synthase I, site-directed mutagenesis, active site mutant, increased activity, decarboxylates malonyl-[acyl-carrier-protein]
D306A
-
synthase I, site-directed mutagenesis, active site mutant, increased activity
E309A
-
synthase I, site-directed mutagenesis, active site mutant, reduced activity
H298A
-
synthase I, site-directed mutagenesis, active site mutant, reduced activity
H298E
-
site-directed mutagenesis, the mutant is decarboxylation deficient, residual decarboxylase activity in the range of pH 6.08.0, crystal structure determination with the mutant enzyme free or bound to C12, comparison to the wild-type enzyme structure
H298Q
-
site-directed mutagenesis, the mutant is completely decarboxylation deficient, crystal structure determination with the mutant enzyme free or bound to C12, comparison to the wild-type enzyme structure
H333A
-
synthase I, site-directed mutagenesis, active site mutant, increased activity
K151Q
-
conserved between FabB, FabF and KasA, proposed to be important for ACP recognition
K328A
-
synthase I, site-directed mutagenesis, active site mutant, reduced activity
K328A
-
site-directed mutagenesis, the mutant is completely decarboxylation deficient, crystal structure determination with the mutant enzyme free or bound to C12, comparison to the wild-type enzyme structure
K328E
-
site-directed mutagenesis, the mutant is almost completely decarboxylation deficient
K328F
-
site-directed mutagenesis, the mutant is completely decarboxylation deficient
K328H
-
site-directed mutagenesis, the mutant is completely decarboxylation deficient
K328I
-
site-directed mutagenesis, the mutant is almost completely decarboxylation deficient
K328R
-
site-directed mutagenesis, the mutant is almost completely decarboxylation deficient, crystal structure determination with the free mutant enzyme, comparison to the wild-type enzyme structure
R62Q
-
conserved between FabB, FabF and KasA, proposed to be important for ACP recognition
C171Q
-
mimics the acyl enzyme intermediate
R161A
-
69.6% of wild-type activity
R46A
-
7.3% of wild-type activity
R46A/R161A
-
0.3% of wild-type activity
T97F
-
no enzymic activity
W42A
-
21.5% of wild-type activity
W42A/R161A
-
0.2% of wild-type activity
C122A
-
site-directed mutagenesis, 75% enhanced production of straight-chain fatty acids relative to branched-chain fatty acids compared to wild-type
C122Q
-
site-directed mutagenesis, 500% enhanced production of straight-chain fatty acids relative to branched-chain fatty acids compared to wild-type
C122S
-
site-directed mutagenesis, enhanced production of straight-chain fatty acids relative to branched-chain fatty acids compared to wild-type
A193M
-
site-directed mutagenesis, hydrophobic acyl-binding pocket is decreased in size by 2.55fold, 12fold reduced catalytic activity
additional information
-
transformation of Brassica napus via Agrobacterium tumefaciens infection with binary vector overexpressing Escherichia coli fabH gene, targeted expression in cytoplasm and plastids is seed specific, modified fatty acid profile of seed oil
K63Q
-
conserved between FabB, FabF and KasA, proposed to be important for ACP recognition
additional information
-
transformation of Brassica napus via Agrobacterium tumefaciens infection with binary vector overexpressing Escherichia coli fabH gene, targeted expression in cytoplasm and plastids is seed specific, modified fatty acid profile of seed oil
additional information
-
introduction of temperature-sensitive mutation in fabB into strain K27 by phage P1-mediated transduction using closely linked Tn10 insertion as selectable marker. A fabB mutant strain accumulates less cis-5-dodecenoic acid than the parental wild-type strain. The wild-type strain also accumulates 2.7fold more cis-7-tetradecenoate than the fabB(Ts) strain. The combination of null mutations in fadD and fabA or fabB is synthetically lethal even in the presence of oleic acid supplementation. A temperature-sensitive mutation is used rather than null mutations because a strain having fabB null mutation has an obligate requirement for an unsaturated fatty acid and FabD is required for uptake of the supplement
R66Q
-
conserved between FabB, FabF and KasA, proposed to be important for ACP recognition
additional information
-
in-frame deletion of enzyme active site region, mutant strain retains about 10% enzymic activity and fails to grow in the absence of supplementation with exogenous long-chain fatty acids. Mutant requires only long-chain unsaturated fatty acids, no saturated fatty acids
C161Q
-
site-directed mutagenesis, active site Cys mutant, no condensation activity, but 377fold increased decarboxylation activity
additional information
-
triple and quadruple mutants of fatty acid synthase complex including mutation sites in the beta-ketoacyl-[acyl-carrier-protein] synthase. e.g. H293A, H331A, K326A,S581A, kinetics, overview
I108F
-
site-directed mutagenesis, hydrophobic acyl-binding pocket is decreased in size by 1.75fold, 38fold reduced catalytic activity towards octanoyl-[acyl-carrier-protein] compared to the activity towards hexanoy-[acyl-carrier-protein]
additional information
Q586W9, -
construction of FAS or ELO enzyme-deficient mutant strains by RNA interference or gene disruption, ACP depletion by RNAi or gene knockout reduces lipoic acid levels and drastically decreases protein lipoylation
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
drug development
-, Q9NWU1
the enzyme is a target for antibacterial drugs
pharmacology
-, P63454
involved in biosynthesis of cell wall mycolic acids, therefor target for discovery of anti-tuberculosis agents
drug development
-
the enzyme is a target for antibacterial drugs
drug development
-
the enzyme is a target for antibacterial drugs
pharmacology
P99159
synthase III is a target for drug development against multi-drug resistant strains
medicine
-
target for the development of drugs for the treatment of cancer and tuberculosis
nutrition
-
target for the engineering of plant seed oils
pharmacology
-
target for the development of drugs for the treatment of cancer and tuberculosis, involved in biosynthesis of precursors of pharmacological agents