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Information on EC 2.1.2.2 - phosphoribosylglycinamide formyltransferase 1 and Organism(s) Pseudomonas aeruginosa

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EC Tree
IUBMB Comments
Two enzymes are known to catalyse the third step in de novo purine biosynthesis. This enzyme utilizes 10-formyltetrahydrofolate as the formyl donor, while the other enzyme, EC 6.3.1.21, phosphoribosylglycinamide formyltransferase 2, utilizes formate. In vertebrates this activity is catalysed by a trifunctional enzyme that also catalyses the activities of EC 6.3.4.13, phosphoribosylamine---glycine ligase and EC 6.3.3.1, phosphoribosylformylglycinamidine cyclo-ligase.
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This record set is specific for:
Pseudomonas aeruginosa
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Word Map
The taxonomic range for the selected organisms is: Pseudomonas aeruginosa
The enzyme appears in selected viruses and cellular organisms
Synonyms
glycinamide ribonucleotide formyltransferase, glycinamide ribonucleotide transformylase, garft, gar tfase, gar transformylase, garftase, phosphoribosylglycinamide formyltransferase, glycinamide ribonucleotide formyl transferase, gar formyltransferase, gartfase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2-amino-N-ribosylacetamide 5'-phosphate transformylase
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-
-
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5'-phosphoribosylglycinamide transformylase
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-
-
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5,10-methenyltetrahydrofolate:2-amino-N-ribosylacetamide ribonucleotide transformylase
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-
-
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ADE8
-
-
-
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GAR formyltransferase
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-
-
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GAR TFase
-
-
-
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GAR transformylase
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-
-
-
GART
-
-
-
-
glycinamide ribonucleotide transformylase
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-
-
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PurN
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
formyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
10-formyltetrahydrofolate:5'-phosphoribosylglycinamide N-formyltransferase
Two enzymes are known to catalyse the third step in de novo purine biosynthesis. This enzyme utilizes 10-formyltetrahydrofolate as the formyl donor, while the other enzyme, EC 6.3.1.21, phosphoribosylglycinamide formyltransferase 2, utilizes formate. In vertebrates this activity is catalysed by a trifunctional enzyme that also catalyses the activities of EC 6.3.4.13, phosphoribosylamine---glycine ligase and EC 6.3.3.1, phosphoribosylformylglycinamidine cyclo-ligase.
CAS REGISTRY NUMBER
COMMENTARY hide
9032-02-4
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
10-formyltetrahydrofolate + N5-hydroxyornithine
tetrahydrofolate + N5-formyl-N5-hydroxyornithine
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
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involved in pyoverdine synthesis
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-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
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expression regulated by amount of iron in the growth medium
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
A0A8G7L7K9_PSEAI
222
0
24714
TrEMBL
-
A0A2X2D459_PSEAI
222
0
24703
TrEMBL
-
A0A8G2YT27_PSEAI
222
0
24761
TrEMBL
-
A0A8G2RI38_PSEAI
216
0
23991
TrEMBL
-
A0A8B4ZH39_PSEAI
222
0
24763
TrEMBL
-
A0A8G4UTU2_PSEAI
222
0
24645
TrEMBL
-
A0A0F6UG69_PSEAI
222
0
24733
TrEMBL
-
A0A5K1SLK7_PSEAI
222
0
24748
TrEMBL
-
A0A2R3IVA3_PSEAI
222
0
24810
TrEMBL
-
A0A071L5M9_PSEAI
222
0
24747
TrEMBL
-
A0A0C7D3G1_PSEAI
222
0
24734
TrEMBL
-
A0A8G7CIJ4_PSEAI
222
0
24675
TrEMBL
-
A0A6A9K892_PSEAI
222
0
24747
TrEMBL
-
A0A431X8Z1_PSEAI
222
0
24711
TrEMBL
-
A0A8G4IE00_PSEAI
222
0
24733
TrEMBL
-
A0A8G4EG01_PSEAI
222
0
24704
TrEMBL
-
A0A8G4A6Q3_PSEAI
222
0
24749
TrEMBL
-
A0A8G5ZL24_PSEAI
222
0
24761
TrEMBL
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30985
-
x * 30985, calculated
31000
-
DNA sequencing
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 30985, calculated
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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enzyme disruption mutant, no synthesis of pyoverdine, mutant strain is unable to grow on King´s B agar containing iron-chelating agent EDTA
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
pvdF gene characterization
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
McMorran, B.J.; Kumara, H.M.C.S.; Sullivan, K.; Lamont, I.L.
Involvement of a transformylase enzyme in siderophore synthesis in Pseudomonas aeruginosa
Microbiology
147
1517-1524
2001
Arabidopsis thaliana, Bacillus subtilis, Saccharomyces cerevisiae, Drosophila melanogaster, Escherichia coli, Pseudomonas aeruginosa
Manually annotated by BRENDA team