Information on EC 2.1.1.77 - protein-L-isoaspartate(D-aspartate) O-methyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Archaea, Bacteria

EC NUMBER
COMMENTARY
2.1.1.77
-
RECOMMENDED NAME
GeneOntology No.
protein-L-isoaspartate(D-aspartate) O-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
D-aspartate (but not L-aspartate) residues in proteins can also act as acceptors
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
methyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:protein-L-isoaspartate O-methyltransferase
D-Aspartate (but not L-aspartate) residues in proteins can also act as acceptors. Previously also listed as EC 2.1.1.24.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D-aspartyl/L-isoaspartyl methyltransferase
-
-
-
-
EC 2.1.1.24
-
-
formerly
-
HPIMT
-
-
isoaspartyl protein carboxyl-O-methyltransferase
-
-
L-aspartyl/L-isoaspartyl protein methyltransferase
-
-
-
-
L-IAMT
-
-
L-isoaspartate O-methyltransferase
-
-
L-isoaspartyl (D-aspartyl) O-methyltransferase
-
-
L-isoaspartyl methyltransferase
-
-
L-isoaspartyl methyltransferase
-
-
L-isoaspartyl methyltransferase
-
-
L-isoaspartyl protein carboxyl methyltransferase
-
-
L-isoaspartyl-O-methyltransferase
-
-
L-isoaspartyl/D-aspartyl O-methyltransferase
-
-
L-isoaspartyl/D-aspartyl protein carboxyl methyltransferase
-
-
-
-
methyltransferase, protein (D-aspartate)
-
-
-
-
PCM-1
Caenorhabditis elegans N2, Caenorhabditis elegans PL51
-
-
-
PCMT
Rattus norvegicus Sprague-Dawley
-
;
-
PIMT
-
-
PIMT
P0A7A5
-
PIMT
-
-
PIMT1
-
isoform
PIMT1
D2IWA7
-
PIMT2 TISIalphaomega
-
isoform
PIMT2 TISIalphapsi
-
isoform
PIMT2 TISIbetapsi
-
isoform
PIMT2 TISIIalphaomega
-
isoform
PIMT2 TISIIalphapsi
-
isoform
PIMT2 TISIIbetapsi
-
isoform
PIMT2 TISIIIalphaomega
-
isoform
PIMT2 TISIIIalphapsi
-
isoform
PIMT2 TISIIIbetapsi
-
isoform
protein (L-isoaspartate) O-methyltransferase
-
-
-
-
protein carboxyl methyltransferase type II
-
-
protein D-aspartate methyltransferase
-
-
-
-
protein isoaspartate methyltransferase
-
-
protein isoaspartate methyltransferase
-
-
protein isoaspartyl carboxyl O-methyltransferase
-
-
protein isoaspartyl methyltransferase
-
-
protein isoaspartyl methyltransferase 1
-
-
protein L-isoaspartate (D-aspartate) O-methyltransferase
-
-
protein L-isoaspartate (D-aspartate) O-methyltransferase
-
-
protein L-isoaspartate methyltransferase
-
-
-
-
protein L-isoaspartate O-methyltransferase
-
-
protein L-isoaspartyl (D-aspartyl) methyltransferase
-
-
protein L-isoaspartyl methyltransferase
-
-
-
-
protein L-isoaspartyl methyltransferase
-
-
protein L-isoaspartyl methyltransferase
Caenorhabditis elegans N2, Caenorhabditis elegans PL51
-
-
-
protein L-isoaspartyl methyltransferase
-
-
protein L-isoaspartyl methyltransferase
P0A7A5
-
protein L-isoaspartyl methyltransferase
-
-
protein L-isoaspartyl methyltransferase
-
-
protein L-isoaspartyl methyltransferase
-
-
protein L-isoaspartyl methyltransferase1
D2IWA7
-
protein L-isoaspartyl-O-methyltransferase
-
-
protein L-isoaspartyl-O-methyltransferase
-
-
protein L-isoaspartyl/D-aspartyl O-methyltransferase
-
-
protein repair L-isoaspartyl methyltransferase
-
-
protein repair L-isoaspartyl methyltransferase 1
-
-
protein-beta-aspartate O-methyltransferase
-
-
-
-
protein-L-isoaspartate methyltransferase
-
-
protein-L-isoaspartate O-methyltransferase
-
-
-
-
type II methyltransferase
-
-
CAS REGISTRY NUMBER
COMMENTARY
105638-50-4
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
mushroom
-
-
Manually annotated by BRENDA team
ecotype Columbia; Thale cress
-
-
Manually annotated by BRENDA team
ecotype Wassilewskija
-
-
Manually annotated by BRENDA team
Heynh.; L. Heynh.
Uniprot
Manually annotated by BRENDA team
Arabidopsis thaliana Heynh.
Heynh.
Uniprot
Manually annotated by BRENDA team
pill woodlouse
-
-
Manually annotated by BRENDA team
Caenorhabditis elegans N2
-
-
-
Manually annotated by BRENDA team
Caenorhabditis elegans N2
strain N2
-
-
Manually annotated by BRENDA team
Caenorhabditis elegans PL51
-
-
-
Manually annotated by BRENDA team
cultivar BGD72
UniProt
Manually annotated by BRENDA team
cultivar Poinsett 76
-
-
Manually annotated by BRENDA team
carrot, var. Danvers Half Long
-
-
Manually annotated by BRENDA team
horse
-
-
Manually annotated by BRENDA team
HeLa cells; human
-
-
Manually annotated by BRENDA team
great slug
-
-
Manually annotated by BRENDA team
no activity in Aquifex aeolicus
-
-
-
Manually annotated by BRENDA team
no activity in Bacillus subtilis
-
-
-
Manually annotated by BRENDA team
no activity in Borrelia burgdorferi
-
-
-
Manually annotated by BRENDA team
no activity in Haemophilus influenzae
-
-
-
Manually annotated by BRENDA team
no activity in Mycoplasma genitalium
-
-
-
Manually annotated by BRENDA team
no activity in Mycoplasma pneumoniae
-
-
-
Manually annotated by BRENDA team
no activity in Sinorhizobium meliloti
-
-
-
Manually annotated by BRENDA team
no activity in Treponema pallidum
-
-
-
Manually annotated by BRENDA team
new Zealand white
-
-
Manually annotated by BRENDA team
rice
-
-
Manually annotated by BRENDA team
rice; strain M-101
-
-
Manually annotated by BRENDA team
Oryza sativa M-101
strain M-101
-
-
Manually annotated by BRENDA team
rat, pheochromocytoma PC-12 line
-
-
Manually annotated by BRENDA team
Sprague-Dawley
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
Sprague-Dawley
-
-
Manually annotated by BRENDA team
tomato, Mill.
-
-
Manually annotated by BRENDA team
wheat; winter wheat, cv Augusta
-
-
Manually annotated by BRENDA team
hybrids White Knight, Kandy Korn and Silver Queen; maize, corn
-
-
Manually annotated by BRENDA team
maize, corn
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
PIMT-knockdown cells show significant accumulation of proteins with isomerized residues, compared to control cells
malfunction
-
reduced PIMT1 accumulation leads to heightened sensitivity to aging treatments and loss of seed vigor under stressful germination conditions
malfunction
-
silencing enzym expression results in the accumulation of proteins with racemized or isomerized Asp residues. Suppression of enzyme expression leads to epidermal growth factor-stimulated hyperactivation of the MAP-kinase
malfunction
-
seed deterioration and loss of viability result from a decrease in enzyme activity
malfunction
-
enzyme depletion upregulates the proapoptotic and checkpoint activation functions of p53
physiological function
-
PIMT repair of abnormal proteins is necessary to maintain normal mitogen-activated protein kinase signaling
physiological function
-
PIMT repairs L-iso-Asp sites in aged nucleosomes
physiological function
-
PCMT1 repairs proteins that have been damaged by deamidation of asparagine or isomerization of aspartate, resulting in the spontaneous generation of L-isoaspartyl residues within peptides and proteins during aging
physiological function
-
worms lacking the pcm-1 gene exhibit reduced longevity as SDS-isolated dauer larvae and as arrested L1 larvae under starvation stress, while overexpression leads to increased adult longevity
physiological function
-
PIMT1 improves longevity and vigor of seeds, PIMT1 overaccumulation reduces the accumulation of L-isoaspartyl residues in seed proteins and increases both seed longevity and germination vigor
physiological function
-, D2IWA7
PIMT1 may have a specifc role in stress adaptation apart from seed
physiological function
-
PCMT maintains the functional stability of antiapoptotic proteins, PCMT when overexpressed in endothelial cells is able to prevent apoptosis induced by H2O2 treatment
physiological function
-
protein L-isoaspartyl/D-aspartyl O-methyltransferase is a repair enzyme that initiates the conversion of L-isoaspartyl (or D-Asp) residues to L-aspartyl residues. Isomerization of Asn or Asp residues, and the repair of atypical residues by PIMT plays a role in the posttranslational regulation of protein activity. The repair of abnormal residues by the enzyme is essential for maintenance of the central nervous system
physiological function
-
the enzyme negatively regulates the tumour suppressor protein p53 by reducing p53 protein levels, thereby suppressing the p53-mediated transcription of target genes. The enzyme destabilizes p53 by enhancing the p53-HDM2 interaction
physiological function
-
enzyme overexpressor adult animals display increased resistance to severe thermal stress (37C). The enzyme plays a signaling role in lifespan extension that involves the insulin/insulin-like growth factor-1 signaling pathway, but is independent of its function in overall protein repair. Enzyme overexpression extends lifespan in a DAF-16-dependent manner under mild thermal stress (25C)
physiological function
Caenorhabditis elegans N2, Caenorhabditis elegans PL51
-
enzyme overexpressor adult animals display increased resistance to severe thermal stress (37C). The enzyme plays a signaling role in lifespan extension that involves the insulin/insulin-like growth factor-1 signaling pathway, but is independent of its function in overall protein repair. Enzyme overexpression extends lifespan in a DAF-16-dependent manner under mild thermal stress (25C)
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 60 S ribosomal protein L27
S-adenosyl-L-homocysteine +
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 7-nitro-2,1,3-benzoxadiazole-delta sleep-inducing peptide-L-isoaspartate
S-adenosyl-L-homocysteine + 7-nitro-2,1,3-benzoxadiazole-delta sleep-inducing peptide-L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 7-nitro-2,1,3-benzoxadiazole-labeled delta sleep-inducing peptide L-isoaspartate
S-adenosyl-L-homocysteine + 7-nitro-2,1,3-benzoxadiazole-labeled delta sleep-inducing peptide L-isoaspartate methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + actin L-isoaspartate
S-adenosyl-L-homocysteine + actin L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + ATHSP17.4 protein
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + Bcl-xL L-isoaspartate
S-adenosyl-L-homocysteine + Bcl-xL L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + beta-101-lysozyme
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + bovine serum albumin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + calmodulin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + calmodulin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + carboxyesterase 12
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + cinnamoyl-CoA reductase family protein
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + delta sleep-inducing peptide
?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + delta-sleep-inducing peptide L-isoaspartate
S-adenosyl-L-homocysteine + delta-sleep-inducing peptide L-aspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + delta-sleep-inducing peptide L-isoaspartate
S-adenosyl-L-homocysteine + delta-sleep-inducing peptide L-isoaspartate alpha-methyl ester
show the reaction diagram
-
delta-sleep-inducing peptide is WAGGDSGE
-
-
?
S-adenosyl-L-methionine + early flowering 8 protein
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + gamma-globulin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + gamma-globulin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + gamma-globulin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
r
S-adenosyl-L-methionine + gamma-globulin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + gelatin I
S-adenosyl-L-homocysteine + ?
show the reaction diagram
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
-
-
-
-
?
S-adenosyl-L-methionine + GFDL-isoD-GGGVG
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + histidine phosphocarrier protein (isoAsp-38)
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + histidine phosphocarrier protein-2 (isoAsp-12,-38)
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + histone H2A L-isoaspartate
S-adenosyl-L-homocysteine + histone H2A L-isoaspartate alpha-methyl ester
show the reaction diagram
-
in chicken, iso-L-Asp occurs at the Asp121-Ser122 bond in the flexible C-terminal tail of histone H2A
-
-
?
S-adenosyl-L-methionine + histone H2B L-isoaspartate
S-adenosyl-L-homocysteine + histone H2B L-isoaspartate alpha-methyl ester
show the reaction diagram
-
in chicken, iso-L-Asp occurs at the Asp26-Lys27 bond in the N-terminal tail of histone H2B
-
-
?
S-adenosyl-L-methionine + histone H2B L-isoaspartate
S-adenosyl-L-homocysteine + histone H2B L-isoaspartate alpha-methyl ester
show the reaction diagram
-
in rat, iso-L-Asp occurs at the Asp25-Gly26 bond in the N-terminal tail of histone H2B
-
-
?
S-adenosyl-L-methionine + Hsp70 L-isoaspartate
S-adenosyl-L-homocysteine + Hsp70 L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + Hsp90 L-isoaspartate
S-adenosyl-L-homocysteine + Hsp90 L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + isoACTH
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
r
S-adenosyl-L-methionine + isoAspDSIP
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + KASA (D-Asp) LAKY
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + KASA (isoD) LAKY
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + KASA (isoD) LAKY
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + KASA (isoD) LAKY
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + KASA (isoD) LAKY
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
synthetic methyl acceptor
-
-
?
S-adenosyl-L-methionine + KASA(iso-D)LAKY
S-adenosyl-L-homocysteine + KASA(isoaspartate alpha-methyl ester)LAKY
show the reaction diagram
-, Q27869
-
-
-
?
S-adenosyl-L-methionine + KASA(iso-D)LAKY
S-adenosyl-L-homocysteine + KASA(L-isoaspartate alpha-methyl ester)LAKY
show the reaction diagram
-, Q27869
-
-
-
?
S-adenosyl-L-methionine + KASA-iso-L-Asp-LAKY
?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + KASA-L-isoAsp-LAKY
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + KASA-L-isoAsp-LAKY
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + KASA-L-isoAsp-LAKY
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + KASA-L-isoAsp-LAKY
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + KASA-L-isoAsp-LAKY
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + KASA-L-isoAsp-LAKY
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + KASA-L-isoAsp-LAKY
S-adenosyl-L-homocysteine + ?
show the reaction diagram
Oryza sativa M-101
-
-
-
-
?
S-adenosyl-L-methionine + KASA-L-isoaspartyl-LAKY
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + KASA-L-isoaspartyl-LAKY
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + KASA-L-isoaspartyl-LAKY
S-adenosyl-L-homocysteine + ?
show the reaction diagram
Caenorhabditis elegans N2, Caenorhabditis elegans PL51
-
-
-
-
?
S-adenosyl-L-methionine + KMK-isoD-TDSEEEIR
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + KQVV-isoD-SAYEVIK
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + L-Val-L-Tyr-L-Pro-(L-iso-Asp)-L-His-L-Ala
S-adenosyl-L-homocysteine + L-Val-L-Tyr-L-Pro-(L-iso-Asp alpha methyl ester)-L-His-L-Ala
show the reaction diagram
-, D2IWA7
-
-
-
?
S-adenosyl-L-methionine + LSH-isoD-GY-Cya-L
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + LSH-isoD-GYCL
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + mitochondrial glycoprotein family protein/MAM33
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + N-succinyl-AAA-p-nitroanilide
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + N-succinyl-AAPF-p-nitroanilide
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + N-succinyl-AAPL-p-nitroanilide
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + N-succinyl-AAV-p-nitroanilide
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + N-succinyl-AAVA-p-nitroanilide
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + native lysozyme
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + oligocellula 2 protein
S-adenosyl-L-homocysteine +
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + ovalbumin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + ovalbumin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + ovalbumin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + ovalbumin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + ovalbumin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + ovalbumin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + ovalbumin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + ovalbumin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + ovalbumin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + ovalbumin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + ovalbumin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + ovalbumin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + ovalbumin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + ovalbumin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
?
S-adenosyl-L-methionine + ovalbumin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
Caenorhabditis elegans N2, Oryza sativa M-101
-
-
-
-
?
S-adenosyl-L-methionine + ovalbumin L-isoaspartate
S-adenosyl-L-homocysteine + ovalbumin L-isoaspartate alpha-methyl ester
show the reaction diagram
-, Q27869
-
-
-
?
S-adenosyl-L-methionine + ovalbumin L-isoaspartate
S-adenosyl-L-homocysteine + ovalbumin L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + ovalbumin L-isoaspartate
S-adenosyl-L-homocysteine + ovalbumin L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + PIRL8 protein
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + PRH75 protein
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein D-aspartate
S-adenosyl-L-homocysteine + protein D-aspartate beta-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein D-aspartate
S-adenosyl-L-homocysteine + protein D-aspartate beta-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein D-aspartate
S-adenosyl-L-homocysteine + protein D-aspartate beta-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein D-aspartate
S-adenosyl-L-homocysteine + protein D-aspartate beta-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein D-aspartate
S-adenosyl-L-homocysteine + protein D-aspartate beta-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein D-aspartate
S-adenosyl-L-homocysteine + protein D-aspartate beta-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein D-aspartate
S-adenosyl-L-homocysteine + protein D-aspartate beta-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein D-aspartate
S-adenosyl-L-homocysteine + protein D-aspartate beta-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein D-aspartate
S-adenosyl-L-homocysteine + protein D-aspartate beta-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein D-aspartate
S-adenosyl-L-homocysteine + protein D-aspartate beta-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein D-aspartate
S-adenosyl-L-homocysteine + protein D-aspartate methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein D-isoaspartate
S-adenosyl-L-homocysteine + protein D-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein D-isoaspartate
S-adenosyl-L-homocysteine + protein D-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein D-isoaspartate
S-adenosyl-L-homocysteine + protein D-isoaspartate alpha-methyl ester
show the reaction diagram
P0A7A5
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
P22061
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
r
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
r
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-, Q8TZR3
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
implicated in the repair or degradation of age-damaged proteins that contain atypical L-isoaspartyl residues
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
post-translational covalent modification
-
-
r
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
key role in degradation or repair of deamination damaged proteins
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
key role in degradation or repair of deamination damaged proteins
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
key role in degradation or repair of deamination damaged proteins
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
function in protein repair
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-, Q8TZR3
protein repair methyltransferase
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
repair enzyme, methylates abnormal L-isoaspartate residues in proteins which arise spontaneously as a result of aging
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
involved in the repair of age-damaged proteins, aspartyl and asparginyl residues that are susceptible to deamidation, isomerization and racemization
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
repair and/or degradation of spontaneously damaged proteins
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
highly active protein repair enzyme
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
repair enzyme that acts upon damaged proteins bearing abnormal aspartyl residues
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
the enzyme can stimulate repair of isoaspartyl residues arising spontaneously in proteins to normal L-aspartyl residues. Protein repair by the enzyme plays a key role in long-term stress survival only at alkaline pH in Escherichia coli, and it may function primarily to repair damage in cells that are recovering from nutrient limitation and those cells that are able to divide during long-term stationary phase
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-, Q27869
the enzyme catalyzes the methylation of L-isoaspartyl residues that arise spontaneously in proteins with age, thereby initiating a repair process that restores the normal backbone configuration to the damaged polypeptide
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
the enzyme might by overburdened in some physiological conditions and its overproduction may be beneficial in conditions in which protein aggregation occrus, for example during biotechnological protein overproduction or in protein aggregation diseases
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
the enzyme prevents the accumulation of deaminated proteins
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
repairs abnormal aspartyl residues accumulated in vivo in type-I collagen and restores cell migration
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
Q8GXQ4
the spontaneous and deleterious conversion of L-asparaginyl and L-aspartyl protein residues to L-iso-Asp or D-Asp occurs as a proteins age and is accelerated under stressful conditions. Arabidopsis thaliana contains two genes (At3g48330 and At5g50240) encoding protein-L-isoaspartate methyltransferase, an enzyme capable of correcting this damage
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
the enzyme might by overburdened in some physiological conditions and its overproduction may be beneficial in conditions in which protein aggregation occurs, for example during biotechnological protein overproduction or in protein aggregation diseases
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
Q8GXQ4
the spontaneous and deleterious conversion of L-asparaginyl and L-aspartyl protein residues to L-iso-Asp or D-Asp occurs as a proteins age and is accelerated under stressful conditions. Arabidopsis thaliana Heynh. contains two genes (At3g48330 and At5g50240) encoding protein-L-isoaspartate methyltransferase, an enzyme capable of correcting this damage
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
PCMT catalyzes methyl esterification of the free alpha-carboxyl group at the isoaspartyl site, thus initiating the repair of the abnormal protein through the conversion of the isopeptide bond into a normal alpha-peptide bond
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
r
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
post-translational covalent modification
-
-
r
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
Caenorhabditis elegans N2
-
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
Caenorhabditis elegans N2
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
Caenorhabditis elegans PL51
-
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
Oryza sativa M-101
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein Lisoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-aspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate beta-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein p53
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
the enzyme mediates methylation of protein p53 at isoaspartate residues 29 and 30
-
-
?
S-adenosyl-L-methionine + succinamide
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + synapsin I
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + T1-isoAsp-12
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + T4-isoAsp-38
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + UDP-xylose synthase 5
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + V2-isoAsp-12
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + V4-isoAsp-38
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP (isoD) HA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP(iso-D)HA
S-adenosyl-L-homocysteine + VYP(isoaspartate alpha-methyl ester)HA
show the reaction diagram
-, Q27869
-
-
-
?
S-adenosyl-L-methionine + VYP(iso-D)HA
S-adenosyl-L-homocysteine + VYP(L-isoaspartate alpha-methyl ester)HA
show the reaction diagram
-, Q27869
-
-
-
?
S-adenosyl-L-methionine + VYP-isoD-CA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-isoD-Cya-G
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-isoD-DA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-isoD-GA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-CA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-GA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-GHG
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-HA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-HA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-HA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-HA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-HA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-HA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-HA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
Oryza sativa M-101
-
-
-
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-RR
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-L-isoaspartyl-HA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + YVS-isoAsp-GHG
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + YVS-isoD-G-Cya-G
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + YVS-isoD-GCG
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + YVS-isoD-GDG
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
-
native E. coli histidine phosphocarrier protein is no substrate
-
-
-
additional information
?
-
-
O-succinyl-L-homoserine, succinyl choline chloride, succinyl acetone, succinic acid and succinamic acid are no substrates
-
-
-
additional information
?
-
-
PIMT level is downregulated by proteasomal degradation, involving the PI3K pathway, during cell attachment. The regulation of PIMT level upon cell-substratum contact suggests a potential role for PIMT in biological processes such as wound healing, cell migration, and tumor metastasis dissemination
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + protein D-aspartate
S-adenosyl-L-homocysteine + protein D-aspartate methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein D-isoaspartate
S-adenosyl-L-homocysteine + protein D-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein D-isoaspartate
S-adenosyl-L-homocysteine + protein D-isoaspartate alpha-methyl ester
show the reaction diagram
P0A7A5
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
P22061
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
r
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
implicated in the repair or degradation of age-damaged proteins that contain atypical L-isoaspartyl residues
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
post-translational covalent modification
-
-
r
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
key role in degradation or repair of deamination damaged proteins
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
key role in degradation or repair of deamination damaged proteins
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
key role in degradation or repair of deamination damaged proteins
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
function in protein repair
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-, Q8TZR3
protein repair methyltransferase
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
repair enzyme, methylates abnormal L-isoaspartate residues in proteins which arise spontaneously as a result of aging
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
involved in the repair of age-damaged proteins, aspartyl and asparginyl residues that are susceptible to deamidation, isomerization and racemization
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
repair and/or degradation of spontaneously damaged proteins
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
highly active protein repair enzyme
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
repair enzyme that acts upon damaged proteins bearing abnormal aspartyl residues
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
the enzyme can stimulate repair of isoaspartyl residues arising spontaneously in proteins to normal L-aspartyl residues. Protein repair by the enzyme plays a key role in long-term stress survival only at alkaline pH in Escherichia coli, and it may function primarily to repair damage in cells that are recovering from nutrient limitation and those cells that are able to divide during long-term stationary phase
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-, Q27869
the enzyme catalyzes the methylation of L-isoaspartyl residues that arise spontaneously in proteins with age, thereby initiating a repair process that restores the normal backbone configuration to the damaged polypeptide
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
the enzyme might by overburdened in some physiological conditions and its overproduction may be beneficial in conditions in which protein aggregation occrus, for example during biotechnological protein overproduction or in protein aggregation diseases
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
the enzyme prevents the accumulation of deaminated proteins
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
the enzyme might by overburdened in some physiological conditions and its overproduction may be beneficial in conditions in which protein aggregation occurs, for example during biotechnological protein overproduction or in protein aggregation diseases
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
PCMT catalyzes methyl esterification of the free alpha-carboxyl group at the isoaspartyl site, thus initiating the repair of the abnormal protein through the conversion of the isopeptide bond into a normal alpha-peptide bond
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein Lisoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
post-translational covalent modification
-
-
r
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
Caenorhabditis elegans N2
-
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
Caenorhabditis elegans N2
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
Caenorhabditis elegans PL51
-
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
Oryza sativa M-101
-
-
-
?
additional information
?
-
-
PIMT level is downregulated by proteasomal degradation, involving the PI3K pathway, during cell attachment. The regulation of PIMT level upon cell-substratum contact suggests a potential role for PIMT in biological processes such as wound healing, cell migration, and tumor metastasis dissemination
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
adenosine
-
tightly bound cofactor
S-adenosyl-L-homocysteine
-
tightly bound cofactor
S-adenosyl-L-homocysteine
-
tightly bound cofactor
S-adenosyl-L-methionine
-, Q8TZR3
active methyl-donating cofactor
S-adenosyl-L-methionine
-
-
S-adenosyl-L-methionine
-
competitive inhibitor of PIMT
S-adenosyl-L-methionine
-
-
S-adenosyl-L-methionine
-
-
S-adenosyl-L-methionine
-, D2IWA7
-
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
5'-deoxy-5'-methylthioadenosine
-
partial inhibition
5'-deoxy-5'-methylthioadenosine
-
-
S-adenosyl-L-homocysteine
-
-
S-adenosyl-L-homocysteine
P0A7A5
-
S-adenosyl-L-homocysteine
-
-
S-adenosyl-L-homocysteine
-
potent inhibitor
S-adenosylhomocysteine
-
complete inhibition
S-adenosylhomocysteine
-
-
S-adenosylhomocysteine
-
-
S-adenosylhomocysteine
-
potent inhibitor at pH 4 and 7.5
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Urea
-
recombinant enzyme relative activity is greatest in liquid assays in 1 M urea
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.055
-
7-nitro-2,1,3-benzoxadiazole-delta sleep-inducing peptide -L-isoaspartate
-
in 0.1 M sodium phosphate (pH 6.8), 1 mM EGTA, 0.004% (w/v) sodium azide, and 0.16% (v/v) Triton X-100, at 37C
0.055
-
7-nitro-2,1,3-benzoxadiazole-labeled delta sleep-inducing peptide L-isoaspartate
-
pH not specified in the publication, at 37C
-
0.183
-
beta-101-lysozyme
-
-
-
0.0035
-
GFDL-isoD-GGGVG
-
-
0.00358
-
histidine phosphocarrier protein-1 (isoAsp-38)
-
-
-
0.197
-
HPr-2 (isoAsp-12,-38)
-
-
-
0.013
-
isoACTH
-
-
0.0164
-
isoAspDSIP
-
-
0.023
-
KASA (D-Asp) LAKY
-
-
2.7
-
KASA (D-Asp) LAKY
-
-
0.00026
-
KASA (isoAsp) LAKY
-
68C, pH 7.5
0.00052
-
KASA (isoAsp) LAKY
-
-
0.00055
-
KASA (isoAsp) LAKY
-
-
0.0028
-
KASA (isoAsp) LAKY
-
-
0.00912
-
KASA (isoAsp) LAKY
-
-
0.0127
-
KASA (isoAsp) LAKY
-
-
0.021
-
KASA (isoAsp) LAKY
-
68C, pH 4.0
0.0506
-
KASA (isoAsp) LAKY
-
-
0.0004
-
KASA (isoD) LAKY
-
-
0.00052
-
KASA (isoD) LAKY
-
-
0.00088
-
KASA (isoD) LAKY
-
-
0.0028
-
KASA (isoD) LAKY
-
37C
0.00912
-
KASA (isoD) LAKY
-
-
0.0127
-
KASA (isoD) LAKY
-
-
0.025
-
KASA (isoD) LAKY
-
85C
0.0506
-
KASA (isoD) LAKY
-
-
0.0145
-
KASA(iso-D)LAKY
Q27869
wild-type enzyme
0.023
-
KASA(iso-D)LAKY
Q27869
mutant enzyme S60Q
0.0286
-
KASA(iso-D)LAKY
Q27869
mutant enzyme S60T
0.08
-
KASA-L-isoAsp-LAKY
-
-
0.092
-
KASA-L-isoAsp-LAKY
-
-
0.00055
-
KQVV-isoD-SAYEVIK
-
-
0.00617
-
KVTCK-isoD-GQTNCYQSK
-
-
0.25
-
L-Val-L-Tyr-L-Pro-(L-iso-Asp)-L-His-L-Ala
-, D2IWA7
in HEPES buffer, pH 7.5, at 37C
0.017
-
LSH-isoD-GY-Cya-L
-
-
0.0036
-
LSH-isoD-GYCL
-
-
-
0.375
-
N-succinyl-AAVAp-nitroanilide
-
68C, pH 7.5
0.69
-
N-succinyl-AAVAp-nitroanilide
-
-
11
-
N-succinyl-AAVAp-nitroanilide
-
68C, pH 4.0
1.081
-
native lysozyme
-
-
-
0.00463
-
ovalbumin
-
68C, pH 7.5
-
0.03
-
ovalbumin
-
-
-
0.035
-
ovalbumin
-
-
-
0.0368
-
ovalbumin
-
-
-
0.166
-
ovalbumin
-
-
-
0.326
-
ovalbumin
-
37C
-
0.326
-
ovalbumin
-
-
-
0.727
-
ovalbumin
-
-
-
1.03
-
ovalbumin
-
-
-
1.2
-
ovalbumin
-
native enzyme
-
1.26
-
ovalbumin
-
in sodium citrate buffer at pH 6.0 and 45C
-
2.32
-
ovalbumin
-
-
-
2.53
-
ovalbumin
-
-
-
2.66
-
ovalbumin
-
-
-
2.77
-
ovalbumin
-
enzyme from seeds
-
4.95
-
ovalbumin
-
-
-
5
-
ovalbumin
-
-
-
12.7
-
ovalbumin
-
enzyme from leaves
-
0.0423
-
ovalbumin L-isoaspartate
Q27869
mutant enzyme S60Q
-
0.121
-
ovalbumin L-isoaspartate
Q27869
wild-type enzyme
-
0.159
-
ovalbumin L-isoaspartate
Q27869
mutant enzyme S60T
-
0.00089
-
S-adenosyl-L-methionine
-
native brain enzyme
0.0011
-
S-adenosyl-L-methionine
-
recombinant protein
0.0019
-
S-adenosyl-L-methionine
-
37C
0.0019
-
S-adenosyl-L-methionine
-
-
0.002
-
S-adenosyl-L-methionine
-
-
0.0022
-
S-adenosyl-L-methionine
-
-
0.0031
-
S-adenosyl-L-methionine
-
-
0.00341
-
S-adenosyl-L-methionine
-
68C, pH 7.5
0.004
-
S-adenosyl-L-methionine
-
brush-border membrane isozyme
0.0056
-
S-adenosyl-L-methionine
-
85C
0.006
-
S-adenosyl-L-methionine
-
-
0.007
-
S-adenosyl-L-methionine
-, D2IWA7
in HEPES buffer, pH 7.5, at 37C
0.03
-
S-adenosyl-L-methionine
-
cytosolic isozyme
0.0012
-
synapsin I
-
-
-
0.0229
-
T1-isoAsp-12
-
-
-
0.339
-
T4-isoAsp-38
-
-
-
0.0274
-
V2-isoAsp-12
-
-
-
0.0975
-
V4-isoAsp-38
-
-
-
0.00029
-
VYP (isoAsp) HA
-
-
-
0.0041
-
VYP (isoAsp) HA
-
-
-
0.0118
-
VYP (isoAsp) HA
-
-
-
0.00029
-
VYP (isoD) HA
-
-
-
0.0041
-
VYP (isoD) HA
-
37C
-
0.0118
-
VYP (isoD) HA
-
-
-
0.0194
-
VYP (isoD) HA
-
-
-
0.0217
-
VYP (isoD) HA
-
-
-
0.0336
-
VYP (isoD) HA
-
85C
-
0.00167
-
VYP-isoD-AA
-
-
-
0.0028
-
VYP-isoD-CA
-
-
-
0.175
-
VYP-isoD-Cya-G
-
-
-
0.00295
-
VYP-isoD-DA
-
-
-
0.00425
-
VYP-isoD-GA
-
-
-
0.0062
-
VYP-isoD-GA
-
-
-
0.00304
-
VYP-isoD-SA
-
-
-
0.0068
-
VYP-L-isoAsp-GA
-
-
-
0.00029
-
VYP-L-isoAsp-HA
-
37C, pH 7.5
-
0.00053
-
VYP-L-isoAsp-HA
-
68C, pH 7.5
-
0.00059
-
VYP-L-isoAsp-HA
-
37C, pH 7.5
-
0.008
-
VYP-L-isoAsp-HA
-
68C, pH 4
-
0.043
-
VYP-L-isoAsp-HA
-
-
-
0.0517
-
VYP-L-isoAsp-HA
-
-
-
0.13
-
VYP-L-isoAsp-HA
-
enzyme from leaves
-
0.24
-
VYP-L-isoAsp-HA
-
-
-
0.29
-
VYP-L-isoAsp-HA
-
enzyme from seeds
-
0.31
-
VYP-L-isoAsp-HA
-
-
-
0.33
-
VYP-L-isoAsp-HA
-
-
-
0.38
-
VYP-L-isoAsp-HA
-
-
-
0.38
-
VYP-L-isoaspartyl-HA
-
in sodium citrate buffer at pH 6.0 and 45C
-
0.005
-
WM-isoD-F-NH2
-
-
-
0.0155
-
YVS-isoAsp-GHG
-
-
-
0.104
-
YVS-isoD-G-Cya-G
-
-
-
0.0046
-
YVS-isoD-GCG
-
-
-
0.469
-
YVS-isoD-GDG
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.7e-06
-
-
-
6.9e-06
-
-
-
1.27e-05
-
-
VYP-L-isoAsp-GHG as substrate
1.75e-05
-
-
-
2.15e-05
-
-
-
0.00013
-
-
gamma-globulin as substrate
0.000229
-
-
VYP-L-isoAsp-RR as substrate
0.000233
-
-
-
0.000433
-
-
ovalbumin as substrate
0.000439
-
-
-
0.001441
-
-
VYP-L-isoAsp-CA as substrate
0.0022
-
-
37C
0.004179
-
-
KASA-L-isoAsp-LAKY as substrate
0.00427
-
-
-
0.005028
-
-
VYP-L-isoAsp-HA as substrate
0.011
-
-
using the delta sleep-inducing peptide as the methyl acceptor
0.012
0.016
-
-
0.0152
-
-
-
0.016
0.02
-
gamma-globulin as methyl acceptor
0.0168
-
-
-
0.021
-
-
recombinant enzyme, at 37C
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
8
-
recombinant enzyme, expressed in Escherichia coli
9
-
-, D2IWA7
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
8
10
-, D2IWA7
-
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
assay at
45
-
-
recombinant enzyme, expressed in E. coli
50
-
-, D2IWA7
-
55
-
-
recombinant enzyme, expressed in E. coli
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
35
55
-, D2IWA7
-
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.6
-
-
isoform PIMT1, isoelectric focusing
6.7
-
-
isoform PIMT2 TISIIIalphaomega, isoelectric focusing; isoform PIMT2 TISIIIalphapsi, isoelectric focusing
7.9
-
-
isoform PIMT2 TISIIIbetapsi, isoelectric focusing
9
-
-
isoform PIMT2 TISIIalphaomega, isoelectric focusing; isoform PIMT2 TISIIalphapsi, isoelectric focusing
9.1
-
-
isoform PIMT2 TISIIbetapsi, isoelectric focusing
9.4
-
-
isoform PIMT2 TISIalphaomega, isoelectric focusing; isoform PIMT2 TISIalphapsi, isoelectric focusing
9.5
-
-
isoform PIMT2 TISIbetapsi, isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
69% reduced PIMT expression as compared to normal brains
Manually annotated by BRENDA team
-
PIMT expression in bovine aortic endothelial cells is regulated by cell detachment and readhesion to a substratum
Manually annotated by BRENDA team
-
enzyme expression and enzyme activity are significantly decreased in all grades of tumors, 76% reduced PIMT expression in pilocytic astrocytomas (grade I), 46% reduced PIMT expression in astrocytomas (grade II), 69% reduced PIMT expression in anaplastic astrocytomas (grade III); enzyme expression and enzyme activity are significantly decreased in all grades of tumors, 76% reduced PIMT expression in pilocytic astrocytomas (grade I), 46% reduced PIMT expression in astrocytomas (grade II), 69% reduced PIMT expression in anaplastic astrocytomas (grade III), 80% reduced PIMT expression in glioblastomas (grade IV) as compared to normal brains
Manually annotated by BRENDA team
-
treatment of astrocytoma cells (U-87) with direct pharmacological GSK-3 inhibitors stimulate PIMT expression (2fold). Results demonstrate regulation of PIMT expression by GSK-3 inhibitor lithium at both the transcriptional and the translational levels. Inhibition by siRNA of GSK-3 and beta-catenin modulates expression of the PIMT in accordance with GSK-3 pharmacological inhibition. Valproic acid up-regulates phospho-GSK-3b (Ser9), beta-catenin and PIMT levels similarly to lithium
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
-
PIMT levels are regulated by cell adhesion in various cancer cell lines. The upregulation of PIMT expression could be prevented by an anti-alphavbeta3 antibody (LM609) or by a cyclic RGD peptide (XJ735) specific to integrin alphavbeta3. PIMT expression returns to the basal level when cells are replated on a substratum after detachment, though downregulation of PIMT expression could be partly prevented by the PI3K inhibitors LY294002 and wortmannin, as well as by the proteasome inhibitors MG-132, lactacystin, and beta-lactone
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
-
80% reduced PIMT expression as compared to normal brains
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
;
-
Manually annotated by BRENDA team
-
; head region, lateral interneurons and tail region
Manually annotated by BRENDA team
-
76% reduced PIMT expression as compared to normal brain
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
-, D2IWA7
PIMT1 is distributed across the organs with maximum levels in seed
Manually annotated by BRENDA team
Oryza sativa M-101
-
-
-
Manually annotated by BRENDA team
Q8GXQ4
PIMT1 expression is more or less similar regardless of stress. PIMT2 transcript abundance increases due to abscisic acid, drought-stress or salt-stress
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Rattus norvegicus Sprague-Dawley
-
;
-
Manually annotated by BRENDA team
-
isoforms PIMT2 TISIalphapsi and PIMT2 TISIalphaomega are predominately present in the chloroplasts, isoform PIMT2 TISIbetapsi is not observed in the chloroplast
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
;
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
-
isoforms PIMT2 TISIbetapsi, PIMT2 TISIalphapsi, and PIMT2 TISIalphaomega are predominately present in small, motile bodies suspected of being mitochondria
Manually annotated by BRENDA team
-
the PIMT2 TISIIalphapsi and alphaomega isoforms are prominent in the nucleus
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Escherichia coli (strain K12)
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Plasmodium falciparum (isolate 3D7)
Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
21000
-
-
brush-border membrane isozyme, SDS-PAGE
23000
-
-
cytosolic isozyme, SDS-PAGE
23300
-
P0A7A5
gel filtration
24000
-
-
gel filtration
24400
-
-
isoform PIMT2 TISIIIalphaomega, SDS-PAGE
24500
-
-
amino acid sequence
24580
-
-
amino acid sequence
24600
-
-
amino acid sequence
24630
-
-
amino acid sequence
24700
-
-
isoform PIMT1, SDS-PAGE
24800
-
-
isoform PIMT2 TISIIIalphapsi, SDS-PAGE
25000
-
-
gel filtration
25000
-
-
gel filtration
25000
-
-
SDS-PAGE
25400
26000
-
-
26500
-
-
SDS-PAGE
26500
-
-
SDS-PAGE
26700
-
-
isoform PIMT2 TISIIIbetapsi, SDS-PAGE
27000
-
-
brain enzyme
28000
-
-
SDS-PAGE
29000
-
-
-
30000
-
-
SDS-PAGE
30200
-
-
isoform PIMT2 TISIIalphaomega, SDS-PAGE
30600
-
-
isoform PIMT2 TISIIalphapsi, SDS-PAGE
32500
-
-
isoform PIMT2 TISIIbetapsi, SDS-PAGE
33700
-
-
isoform PIMT2 TISIalphaomega, SDS-PAGE
34000
-
-
SDS-PAGE
34100
-
-
isoform PIMT2 TISIalphapsi, SDS-PAGE
35000
-
-
thymus enzyme
35000
-
-
SDS-PAGE
36000
-
-
isoform PIMT2 TISIbetapsi, SDS-PAGE
36400
-
-
calculated from translated amino acid sequence
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hexamer
-
cooperative hexamerization occurs via interactions of hot spot' residues; cooperative hexamerization occurs via interactions of hot spot residues. A couple of interfacial hot spot residues are responsible for enhancing thermostability via oligomerization
monomer
-
1 * 26500, isoenzyme I and II, SDS-PAGE
monomer
-
1 * 26500, SDS-PAGE
monomer
-
1 * 24000, SDS-PAGE
monomer
-
-
monomer
-
1 + 25000
monomer
-
1 * 34000, equilibrium centrifugation
monomer
-
1 * 28000, SDS-PAGE
monomer
P0A7A5
1 * 21900, calculated from amino acid sequence
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
sitting drop vapour diffusion method, resolution of 2.2 A
Q27869
in complex with S-adenosyl-L-homocysteine, hanging drop vapor diffusion method, using 0.1 M HEPES-Na [pH 7.5], 0.8 M monosodium dihydrogen phosphate, and 0.8 M monopotassium dihydrogen phosphate, at 15C
P0A7A5
hanging drop vapor diffusion method, crystal structure at 2.8 A resolution. The monomeric structure of StoPIMT consists of two domains, an AdoMet-dependent methyltransferase fold domain and a distinctive C-terminal alpha-helical domain. Six monomers associate into a hexamer, in which there are three contact regions per monomer, referred to as the major, minor, and C-terminal alpha-helical contact regions; hanging-drop vapour diffusion. Crystal structure shows that the enzyme has a distinctive hexameric structure composed of monomers consisting of two domains and a C-terminal alpha-helical domain
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
9.5
-
-, D2IWA7
above pH 9.5 the enzyme activity rapidly declines
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
35
50
-
enzyme from leaves
35
65
-
enzyme from seeds
37
50
-
-
55
-
-
loses activity rapidly at
60
-
-, D2IWA7
at temperature above 60C, PIMT activity shows a sharp decline
85
95
-
little or no loss of activity
100
-
-
extremely heat stable, no loss of activity after 60 min
additional information
-
-
a couple of interfacial hot spot residues are responsible for enhancing thermostability via oligomerization
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
the Ile isoform of PCMT is more stable, but the Val isoform has a 30% higher affinity for protein substrates
-
no stability problems during purification
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-70C, can be stored in 10% glycerol
-
-80C, stored in liquid nitrogen, no apparent loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
MagneHis protein purification system
-
nickel column chromatography
-
recombinant protein
-
2 isozymes
-
recombinant protein
-
nickel-charged affnity column chromatography
-, D2IWA7
chelating Sepharose column chromatography
P0A7A5
2 isozymes
-
His GraviTrap column chromatography
-
nickel column affinity chromatography
-
recombinant protein
-
recombinant protein
-
recombinant protein
-
recombinant protein
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
cDNA isolation, pcm gene cloned and expressed in Escherichia coli BL21(DE3)
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(DE3)RIL cells
-
disruption of the pcm gene
-
expressed in Escherichia coli BL21(DE3) cells
-, D2IWA7
overexpression in transgenic flies extends the normal life span, suggesting that protein damage can be a limiting factor in longevity
Q27869
expressed in Escherichia coli strain B834 (DE3)
P0A7A5
overexpression in Escherichia coli
-
overexpression of the enzyme in Escherichia coli increases heat shock survival by a mechanism independent of methyltransferase activity
-
pcm gene sequenced
-
expressed in Escherichia coli BL21(DE3)pLysS cells
-
expressed in porcine aortic endothelial cells
-
His-tagged enzyme is expressed in Escherichia coli
-
southern blot analysis of PCR amplified genomic DNA, human chromosome contains a single gene encoding the enzyme, PCMT1, mapped to the q24-25 region of chromosome 6
-
cloned and expressed in Escherichia coli TOP10
-
PCR amplified cDNA cloned into vector pdeltablue and expressed in Escherichia coli
-
sequence analysis of a cDNA clone of a rat brain enzyme
-
pcm gene PCR amplified, overexpressed in Escherichia coli as a fusion protein
-
cDNA isolation
-
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
pcm-1 dauer and dauer-like larvae display an average of 46% decrease in lifespan in comparison to N2 dauer and dauer-like larvae
-
upon heat shock and dauer formation-inducing conditions, there is additional pcm-1 expression in body wall muscle nuclei and actomyosin filaments and in hypodermal cells
-
the activity is elevated about 3fold towards high temperature, cold, salt, dehydration, and oxidative stress induced by paraquat, as well as high pH conditions
-, D2IWA7
enzyme expression decreases sharply as the seed undergoes dehydration
-
down-regulation of PIMT by siRNA strikingly enhances phenylarsine oxide-induced reactive oxygen species
-
enzyme activity and expression are 50% lower in the hippocampus of epileptic patients
-
1-chloro-2,4-dinitrobenzene does not affect PIMT expression
-
PIMT is about 2fold up-regulated by 0.005 mM phenylarsine oxide in U-87 human astroglioma cells, PIMT up-regulation by phenylarsine oxide is mediated by the reaction with vicinal cysteines, PIMT induction by phenylarsine oxide is dependent on formation of reactive oxygen species, PIMT induction by phenylarsine oxide is inhibited by N-acetyl-L-cysteine and diphenyleneiodonium chloride; PIMT is slightly up-regulated by 0.01 mM cycloheximide
-
5 mM valproic acid enhances protein L-isoaspartyl methyltransferase expression about 2fold through glycogen synthase kinase-3/beta-catenin requires 90 kDa ribosomal S6 kinase. Src, c-Raf, MEK1/2 and extracellular-regulated kinase 1/2 abolish the extracellular-regulated kinase 1/2 phosphorylation stimulated by valproic acid, thus preventing PIMT induction by valproic acid
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
S60A
Q27869
inactive mutant protein
S60Q
Q27869
the ratio of turnover number to KM-value for ovalbumin L-isoaspartate is 24.3fold lower than the wild-type value, the ratio of turnover-number to KM-value for KASA(iso-D)LAKY is 21fold lower than wild-type value
S60T
Q27869
the ratio of turnover number to KM-value for ovalbumin L-isoaspartate is 6.2fold lower than the wild-type value, the ratio of turnover-number to KM-value for KASA(iso-D)LAKY is 2fold lower than the wild-type value
D83F
-
negative dominant mutant
D83V
-
negative dominant mutant
DELTA206-231
-
hexameric structure and thermostability retained
V119I
P22061
the variant enzyme has lower activity and thermal stability but about 30% increased affinity for endogenous substrates compared to the I119I variant
additional information
-
livers of male Wistar rats, fed the Lieber DeCarli control, ethanol or 1% betaine-supplemented diets for 4 weeks, are processed for PIMT-related analyses. A significant increase in the accumulation of modified proteins bearing isoaspartyl residues (substrates for PIMT), in homogenate samples and various subcellular fractions of livers from ethanol-fed rats are observed. Betaine supplementation prevents this accumulation of damaged proteins. Ethanol exposure induces no changes in the PIMT enzyme activity levels as compared to controls. The accumulation of damaged proteins negatively correlates with hepatic S-adenosylmethionine to S-adenosylhomocysteine ratios
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
L-isoaspartyl residue formation in extracellular matrix proteins such as type -I collagen could play an important role in reducing cell migration. PIMT could be a therapeutic tool to restore normal cell migration in pathological conditions where cell motility is crucial
nutrition
-
enzymatic detection of L-isoaspartyl residues in food proteins
medicine
-
aging research, accumulation of damaged proteins have detrimental effects over the human lifespan
medicine
-
results show that PIMT expression is up-regulated by GSK-3 inhibition and beta-catenin stabilization upon treatments with lithium and valproic acid suggesting a possible therapeutic role for PIMT in certain brain diseases including epilepsy
medicine
-
mutations in the PCMT1 gene are associated with premature ovarian failure
medicine
-
PCMT1 is associated with the type 1 diabetes in groups defined by other risk genes (SUMO4 and HLA)
medicine
-
a cloned source of this enzyme should be useful in the identification of cellular substrates and for quantitation and localization of isoaspartyl sites in purified proteins, including recombinant proteins used as pharmaceuticals
molecular biology
-
betaine administration of rats prevents the ethanol-induced accumulation of isoaspartyl-containing proteins in the liver by restoring the PIMT-catalyzed protein repair reaction through normalizing the hepatocellular SAM:SAH ratios
biotechnology
-
useful in biotechnical applications