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Information on EC 2.1.1.37 - DNA (cytosine-5-)-methyltransferase and Organism(s) Homo sapiens

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EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.37 DNA (cytosine-5-)-methyltransferase
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Select one or more organisms in this record: ?
This record set is specific for:
Homo sapiens
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Synonyms
dnmt1, dnmt3a, dna methyltransferase, dnmt3b, dna methyltransferase 1, dnmt2, dna mtase, nsun2, dna methylase, dna methyltransferase 3a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5-cytosine DNA methyltransferase
-
-
C5-MTase
-
-
-
-
cytosine 5-methyltransferase
-
-
-
-
cytosine carbon 5 DNA methyltransferase
-
cytosine DNA methylase
-
-
-
-
cytosine DNA methyltransferase
cytosine-specific DNA methyltransferase
-
-
-
-
DCMT
-
-
-
-
deoxyribonucleate methylase
-
-
-
-
deoxyribonucleate methyltransferase
-
-
-
-
deoxyribonucleic (cytosine-5-)-methyltransferase
-
-
-
-
deoxyribonucleic acid (cytosine-5-)-methyltransferase
-
-
-
-
deoxyribonucleic acid methylase
-
-
-
-
deoxyribonucleic acid methyltransferase
-
-
-
-
deoxyribonucleic acid modification methylase
-
-
-
-
deoxyribonucleic methylase
-
-
-
-
DNA (cytosine-5) methyltransferase
-
-
DNA (cytosine-5)-methyltransferase 1
-
-
DNA (cytosine-5-)-methyltransferase 3A
-
-
DNA 5-cytosine methylase
-
-
-
-
DNA cytosine c5 methylase
-
-
-
-
DNA cytosine methylase
-
-
-
-
DNA cytosine-5 methyltransferase 1
-
DNA cytosine-5-methyltransferase 1
-
-
DNA cytosine-5-methyltransferase 3A
-
-
DNA cytosine-5-methyltransferase 3B
-
-
DNA methylase
-
-
-
-
DNA methyltransferase
DNA methyltransferase 1
DNA methyltransferase 3a
-
DNA transmethylase
-
-
-
-
DNA-cytosine 5-methylase
-
-
-
-
DNA-cytosine methyltransferase
-
-
-
-
Dnmt1
Dnmt2
-
-
Dnmt3a
DNMT3B
DNMT3b2
isoform
DNMTB
-
-
EcoRI methylase
-
-
-
-
hDNMT1
-
-
methylphosphotriester-DNA methyltransferase
-
-
-
-
methyltransferase, deoxyribonucleate
-
-
-
-
type II DNA methylase
-
-
-
-
additional information
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + DNA containing cytosine = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:DNA (cytosine-5-)-methyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9037-42-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
show the reaction diagram
S-adenosyl-L-methionine + (CGG*CCG)12
?
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + (CGG*CCG)73
?
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + (CGG*CGG)12
?
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + (CMeGG-CCG)12
S-adenosyl-L-homocysteine + (CMeGG-CCG)12 containing 5-methylcytosine
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
show the reaction diagram
S-adenosyl-L-methionine + linear pRW 3602 DNA containing cytosine
S-adenosyl-L-homocysteine + linear pRW 3602 DNA containing 5-methylcytosine
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + Micrococcus luteus DNA containing cytosine
S-adenosyl-L-homocysteine + Micrococcus luteus DNA containing 5-methylcytosine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + mononucleosomal DNA containing cytosine
S-adenosyl-L-homocysteine + mononucleosomal DNA containing 5-methylcytosine
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + native Micrococcus lysodeikticus DNA containing cytosine
S-adenosyl-L-homocysteine + native Micrococcus lysodeikticus DNA containing 5-methylcytosine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + P815 DNA containing cytosine
S-adenosyl-L-homocysteine + P815 DNA containing 5-methylcytosine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + poly (dI-dC)
S-adenosyl-L-homocysteine + poly (dI-dC) containing 5-methylcytosine
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + poly(dI-dC)*poly(dI-dC)
?
show the reaction diagram
S-adenosyl-L-methionine + poly(dI-dC)/poly(dI-dC)
S-adenosyl-L-homocysteine + poly(dI-dC)/poly(dI-dC) containing 5-methylcytosine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + poly-(dI-dC)*poly(dI-dC)
?
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + poly-(dI-dC)/poly(dI-dC)
?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + relaxed circular pRW 3602 DNA containing cytosine
S-adenosyl-L-homocysteine + relaxed circular pRW 3602 DNA containing 5-methylcytosine
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + supercoiled pRW 3602 DNA containing cytosine
S-adenosyl-L-homocysteine + supercoiled pRW 3602 DNA containing 5-methylcytosine
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
show the reaction diagram
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(-)-epigallocatechin-3-gallate
-
competitive, the inhibitor can form hydrogen bonds with Pro1223, Glu1265, Cys1225, Ser1229 and Arg1309. Hypermethylation of CpG islands in the promoter regions is an important mechanism to silence the expression of many important genes in cancer. (-)-Epigallocatechin-3-gallate can inhibit DNMT activity and reactivate methylation-silenced genes in cancer cells
(N4-fluoroacetyl-5-azacytidine)
efficient inhibitor of DNA methylation in human tumor cell lines
2'-dC
-
binding modelling, overview
2'-deoxy-5-aza-cytidine
-
binding modelling, overview
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-chloro-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
-
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-fluoro-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
-
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-iodo-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
-
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-methoxy-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
-
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-methyl-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
-
2-amino-4-[([(2S,3S,4R,5R)-5-[6-amino-2-(methylsulfanyl)-9H-purin-9-yl]-3,4-dihydroxytetrahydrofuran-2-yl]methyl)sulfanyl]butanoic acid
-
2-pyrimidinone
forms a part of inhibitor 1,2-dihydropyrimidin-2-one-5-methylene-(methylsulfonium)-adenosyl, bindng structure and mechanism, overview
5-aza-2'-deoxycytidine
-
-
5-aza-cytosine
-
5-azacytidine
-
binding modelling, overview
5-fluorocytosine
-
6-thioguanine
-
incorporation of 6-thioguanine perturbs cytosine methylation at the CpG dinucleotide site by DNA methyltransferases in vitro and acts as a DNA demethylating agent in vivo. Presence of 6-thioguanine at the unmethylated CpG site abolished almost completely the methylation of its 5' adjacent cytosine by DNMT1
asCEBPA-1 RNA
competitive with respect to substrate (CMeGG-CCG)12
-
asCEBPA-2 RNA
mixed inhibition
-
asCEPA-1HPE RNA
competitive with respect to substrate (CMeGG-CCG)12
-
asCEPA-2HPE RNA
competitive with respect to substrate (CMeGG-CCG)12
-
curcumin
-
-
decitabine
-
binding modelling, overview
EDTA
-
above 10 mM
hydralazine
iodoacetamide
-
-
miR-127-3p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-145-5p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-146a-5p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-155-5p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12; micro-RNA. Exogenous miR-155-5p in cells inhibits DNMT1 and induces aberrant DNA methylation of the genome
-
miR-16-5p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-17-5p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-19b-3p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-20a-5p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-21-5p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-373-5p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-9-3p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-9-5p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-92-1-5p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-92a-1-5p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-92a-3p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
NSC 106084
-
inhibition of Dnmt1
NSC 137546
-
inhibition of Dnmt1
NSC 138419
-
inhibition of Dnmt1
NSC 14778
-
inhibition of Dnmt1; inhibition of Dnmt3b
NSC 158324
-
inhibition of Dnmt1
NSC 319745
-
inhibition of Dnmt1
NSC 348926
-
slight inhibition of Dnmt1
NSC 408488
-
inhibition of Dnmt1
NSC 54162
-
inhibition of Dnmt1
NSC 56071
-
inhibition of Dnmt1
NSC 57893
-
inhibition of Dnmt1
NSC 622444
-
inhibition of Dnmt1
poly[d(G-5-azacytidine)]
-
-
-
procainamide
-
binding modelling, overview
procaine
-
binding modelling, overview
retinoblastoma gene product
-
a negative regulator of DNA methylation, binds to the allosteric site of hDNMT1 and inhibits methylation, it may regulate methylation spreading
-
RG108
-
slight inhibition of Dnmt1
S-(1-deazaadenosyl)-L-homocysteine
-
S-(3-deazaadenosyl)-L-homocysteine
-
S-(8-azaadenosyl)-L-homocysteine
-
S-(N-(2-biphenyl-4-ylethyl)-2-chloroadenosyl)-L-homocysteine
-
S-(N-(2-biphenyl-4-ylethyl)adenosyl)-L-homocysteine
-
S-(N-(3,5-dimethoxybenzyl)adenosyl)-L-homocysteine
-
S-(N-(pyridin-4-ylmethyl)adenosyl)-L-homocysteine
-
S-(N-benzyladenosyl)-L-homocysteine
-
S-(N-phenyladenosyl)-L-homocysteine
-
S-(N-phenylethyladenosyl)-L-homocysteine
-
S-(N-phenylpropyladenosyl)-L-homocysteine
-
S-adenosyl-L-homocysteine
S-inosinylhomocysteine
-
S-nebularinehomocysteine
-
S-Tubercidinylhomocysteine
-
[1,2-dihydropyrimidin-2-one]-5-methylene-(methylsulfonium)-adenosyl
with 2-pyrimidinone ring
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dimethyl sulfoxide
stimulates
methylated DNA
-
stimulated methylation spreading on unmethylated CpG sequences for full-length and the mutant lacking 121 N-terminal amino acids equally well. No stimulation of N-terminal deletion mutants lacking 501, 540, or 580 amino acids from the N-terminus
-
NSC 319745
-
activation of Dnmt3b
NSC 345763
-
slight activation of Dnmt1
RNAse A
RNase A treatment increases DNMT1 activity in vitro. The methyltransferase activity on hemimethylated DNA is about 8fold higher compared to control, and 2fold higher on a poly(dI-dC) substrate
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0008
(CGG*CCG)12
-
-
0.0001
(CGG*CCG)73
-
-
0.041 - 0.092
DNA
0.02
linear pRW 3602 DNA containing cytosine
-
-
0.086
Micrococcus luteus DNA containing cytosine
-
-
-
0.52 - 1.4
mononucleosomal DNA containing cytosine
-
0.0077
native Micrococcus lysodeikticus DNA containing cytosine
-
-
-
0.015
P815 DNA containing cytosine
-
-
-
0.0005 - 0.0007
poly(dI-dC)*poly(dI-dC)
-
0.0005 - 0.00082
poly-(dI-dC)/poly(dI-dC)
-
0.003
relaxed circular pRW 3602 DNA containing cytosine
-
-
0.00325 - 0.014
S-adenosyl-L-methionine
0.023
supercoiled pRW 3602 DNA containing cytosine
-
-
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00055
(CGG*CCG)73
-
-
0.00305
(CGG*CGG)12
-
-
0.00167
linear pRW 3602 DNA containing cytosine
-
-
0.145
poly(dI-dC)*poly(dI-dC)
-
-
0.0511
poly(dI-dC)poly(dI-dC)
-
-
-
0.013 - 0.058
poly-(dI-dC)/poly(dI-dC)
-
0.00055
relaxed circular pRW 3602 DNA containing cytosine
-
-
0.013 - 0.058
S-adenosyl-L-methionine
0.00278
supercoiled pRW 3602 DNA containing cytosine
-
-
additional information
additional information
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00689
(-)-epigallocatechin-3-gallate
-
substrate: poly(dI-dC)/poly(dI-dC)
0.002
asCEBPA-1 RNA
substrate (CMeGG-CCG)12, pH 8.0, 37°C
-
0.00009 - 0.00043
asCEBPA-2 RNA
-
0.00092
asCEPA-1HPE RNA
substrate (CMeGG-CCG)12, pH 8.0, 37°C
-
0.000135
asCEPA-2HPE RNA
substrate (CMeGG-CCG)12, pH 8.0, 37°C
-
0.000269
miR-127-3p
substrate (CMeGG-CCG)12, pH 8.0, 37°C
-
0.002458
miR-145-5p
substrate (CMeGG-CCG)12, pH 8.0, 37°C
-
0.001031
miR-146a-5p
substrate (CMeGG-CCG)12, pH 8.0, 37°C
-
0.000028
miR-155-5p
substrate (CMeGG-CCG)12, pH 8.0, 37°C
-
0.000463
miR-16-5p
substrate (CMeGG-CCG)12, pH 8.0, 37°C
-
0.000317
miR-17-5p
substrate (CMeGG-CCG)12, pH 8.0, 37°C
-
0.003323
miR-19b-3p
substrate (CMeGG-CCG)12, pH 8.0, 37°C
-
0.000561
miR-20a-5p
substrate (CMeGG-CCG)12, pH 8.0, 37°C
-
0.001291
miR-21-5p
substrate (CMeGG-CCG)12, pH 8.0, 37°C
-
0.000298
miR-373-5p
substrate (CMeGG-CCG)12, pH 8.0, 37°C
-
0.001602
miR-9-3p
substrate (CMeGG-CCG)12, pH 8.0, 37°C
-
0.000493
miR-9-5p
substrate (CMeGG-CCG)12, pH 8.0, 37°C
-
0.000476
miR-92a-1-5p
substrate (CMeGG-CCG)12, pH 8.0, 37°C
-
0.00165
miR-92a-3p
substrate (CMeGG-CCG)12, pH 8.0, 37°C
-
additional information
additional information
inhibition by mechanism-based inhibitors and kinetics, detailed overview
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.045
1-deaza-S-adenosyl-L-homocysteine
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.0029 - 0.0572
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-chloro-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
0.0025 - 0.0068
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-fluoro-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
0.021 - 0.256
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-iodo-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
0.075
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-methoxy-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.001 - 0.006
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-methyl-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
0.216 - 0.5
2-amino-4-[([(2S,3S,4R,5R)-5-[6-amino-2-(methylsulfanyl)-9H-purin-9-yl]-3,4-dihydroxytetrahydrofuran-2-yl]methyl)sulfanyl]butanoic acid
0.045
3-deaza-S-adenosyl-L-homocysteine
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.008
8-aza-S-adenosyl-L-homocysteine
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.017 - 0.092
NSC 14778
0.045
S-(1-deazaadenosyl)-L-homocysteine
Homo sapiens
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.0068
S-(3-deazaadenosyl)-L-homocysteine
Homo sapiens
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.003
S-(8-azaadenosyl)-L-homocysteine
Homo sapiens
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.0067 - 0.029
S-(N-(2-biphenyl-4-ylethyl)-2-chloroadenosyl)-L-homocysteine
0.0006 - 0.0054
S-(N-(2-biphenyl-4-ylethyl)adenosyl)-L-homocysteine
0.0009 - 0.018
S-(N-(3,5-dimethoxybenzyl)adenosyl)-L-homocysteine
0.0036 - 0.022
S-(N-(pyridin-4-ylmethyl)adenosyl)-L-homocysteine
0.001 - 0.061
S-(N-benzyladenosyl)-L-homocysteine
0.002 - 0.154
S-(N-phenyladenosyl)-L-homocysteine
0.002 - 0.027
S-(N-phenylethyladenosyl)-L-homocysteine
0.0006 - 0.0074
S-(N-phenylpropyladenosyl)-L-homocysteine
0.0003 - 0.002
S-adenosyl-L-homocysteine
1
S-inosinylhomocysteine
0.028 - 0.3
S-nebularinehomocysteine
0.0003 - 0.0015
S-Tubercidinylhomocysteine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000013
-
-
0.0000166
-
-
0.00115
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.5
-
-
7.8
assay at
8
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
assay at
additional information
-
assay carried out at room temperature
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
cell line SKGT4
Manually annotated by BRENDA team
-
colon cancer
Manually annotated by BRENDA team
-
esophageal cancer
Manually annotated by BRENDA team
neoplastically-transformed
Manually annotated by BRENDA team
-
prostate cancer
Manually annotated by BRENDA team
-
a human colorectal carcinoma cell line
Manually annotated by BRENDA team
-
immunohistochemical analysis
Manually annotated by BRENDA team
-
pleomorphic adenoma, adenoid cystic carcinoma, mucoepidermoid carcinoma and polymorphous low-grade adenocarcinoma of lower lip, buccal mucosa, mandible, tongue, floor, or palate. Positive nuclear and cytoplasmic labeling for DNMT1 occurs in all samples, positive nuclear labeling for DNMT3a occurs only in few neoplasms: 1 pleomorphic adenoma, 2 adenoid cystic carcinoma and 1 mucoepidermoid cases, immunohistochemical
Manually annotated by BRENDA team
-
cell lines TE-3 and TE-12
Manually annotated by BRENDA team
-
CD4+, ranscript levels of DNA methyltransferases DNMT1, DNMT3A and DNMT3B in CD4+ T cells from patients with systemic lupus erythematosus, an autoimmune disease with CD4+ T cells with hypomethylated DNA. It is possible that patients are reacting indirectly to an underlying DNA hypomethylation status by increasing the mRNA levels of DNA methyltransferases when the disease is definitely active, overview
Manually annotated by BRENDA team
-
B lymphoblast cell line containing wild-type p53. The interaction between p53 and DNMT1 controls DNA methylation of the p16ink4A promoter that consequently reduces the level of the p16ink4A
Manually annotated by BRENDA team
-
B lymphoblast cell line with mutant p53. Mutant p53 loses its ability to suppress DNMT1 expression, and thus enhances methylation levels of the p16ink4A promoter and subsequently down-regulates p16ink4A protein
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
both DNMT3A and DNMT3B are involved in de novo DNA methylation
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DNMT1_HUMAN
1616
0
183165
Swiss-Prot
Mitochondrion (Reliability: 4)
DNM3A_HUMAN
912
0
101858
Swiss-Prot
other Location (Reliability: 2)
DNM3B_HUMAN
853
0
95751
Swiss-Prot
other Location (Reliability: 1)
A0A7I2V490_HUMAN
1658
0
187311
TrEMBL
Mitochondrion (Reliability: 4)
A0A7I2V311_HUMAN
1150
0
130486
TrEMBL
other Location (Reliability: 2)
Q59FP7_HUMAN
1606
0
182020
TrEMBL
other Location (Reliability: 2)
A0A7I2YQA8_HUMAN
1577
0
179163
TrEMBL
Mitochondrion (Reliability: 4)
Q59HC6_HUMAN
811
0
89707
TrEMBL
other Location (Reliability: 5)
A0A0C4DG02_HUMAN
689
0
77817
TrEMBL
other Location (Reliability: 1)
A0A7I2V5F1_HUMAN
1580
0
179380
TrEMBL
Mitochondrion (Reliability: 4)
B3KVA0_HUMAN
540
0
60153
TrEMBL
other Location (Reliability: 2)
A0A7I2YQQ0_HUMAN
1613
0
182353
TrEMBL
Mitochondrion (Reliability: 4)
A0A2Z2CIT2_HUMAN
780
0
87327
TrEMBL
other Location (Reliability: 1)
A0A7I2V5F6_HUMAN
1114
0
127143
TrEMBL
Mitochondrion (Reliability: 4)
A0A024R7E3_HUMAN
1678
0
189566
TrEMBL
other Location (Reliability: 3)
F8WE91_HUMAN
781
0
86722
TrEMBL
other Location (Reliability: 2)
A0A7I2V5D0_HUMAN
1655
0
187094
TrEMBL
Mitochondrion (Reliability: 4)
I6L9H2_HUMAN
1511
0
171073
TrEMBL
other Location (Reliability: 2)
H31_HUMAN
136
0
15404
Swiss-Prot
-
TRDMT_HUMAN
391
0
44597
Swiss-Prot
-
PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
120000
-
glycerol density gradient sedimentation
135000
-
glycerol gradient centrifugation
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
-
IL-6 enhances the nuclear translocation of DNA cytosine-5-methyltransferase 1 (DNMT1) via phosphorylation of the nuclear localization sequence by the AKT kinase
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C667G
zinc-finger-domain point mutation in the CXXC motif abolishes unmethylated DNA binding activity
C667G/C670G
zinc-finger-domain point mutation in the CXXC motif abolishes unmethylated DNA binding activity
C670G
zinc-finger-domain point mutation in the CXXC motif abolishes unmethylated DNA binding activity
DELAT1-501
-
to accelerate the rate of DNMT1 with the hemimethylated substrate, the 501 amino acids at the N terminus are deleted. Truncated DNMT1 exhibits a 4fold increased kcat/Km as compared with the full-length enzyme and does not require allosteric activation by hemimethylated DNA
DELTA1-501
-
no stimulation by methylated DNA
DELTA1-540
-
no stimulation by methylated DNA
DELTA1-580
-
no stimulation by methylated DNA
H286A/R287A
-
allosteric activation by methylated DNA is reduced, 6.5 fold decrease in the ratio turnover number/Km-value compared to wild-type enzyme
K284A/K285A
-
allosteric activation by methylated DNA is reduced, 1.7fold decrease in the ratio turnover number/Km-value compared to wild-type enzyme
additional information
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
rather unstable
65
-
20 min, inactivation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
glycerol stabilizes
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, stable for at least several weeks
-
-80°C, 30% glycerol, activity decays at a rate of about 15% in 2 weeks
-
-80°C, little loss of activity after 4 weeks, significant loss of activity after 6 weeks
-
-80°C, stable for at least 3 weeks
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNMT3B copurifies with several components of the condensin complex and KIF4A
Hitrap SP-Sepharose column chromatography
Q-Sepharose column chromatography and Hitrap heparin column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNMT1 and DNMT3a, expression analysis of the isozymes in salivary gland neoplasmata
-
DNMT1, overexpression of HA-tagged CXXC deletion DNMT1 mutant, expression of wild-type and deletion mutant enzymes in COS-7 cells and Escherichia coli, CXXC deleted DNMT1, mutant DNMT1DELTACXXC, is localized on replication foci
expressed in High Five insect cells
expression in Sf9 cells
expression of the enzyme by using a protein splicing fusion partner in a baculovirus expression vector
-
transcript levels of DNA methyltransferases DNMT1, DNMT3A and DNMT3B in CD4+ T cells from patients with systemic lupus erythematosus, phenotype, overview
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
-
using a truncated and highly active form of human DNMT1 and a designed hemimethylated DNA substrate, a robust, efficient, and economical fluorescence assay is developped suitable for in vitro high-throughput screening of DNMT1
drug development
-
Dnmt inhibition is a promising strategy for the treatment of various developmental and proliferative diseases, particularly cancers
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Roy, P.H.; Weissbach, A.
DNA methylase from HeLa cell nuclei
Nucleic Acids Res.
2
1669-1684
1975
Homo sapiens
Manually annotated by BRENDA team
Pfeifer, G.P.; Grunwald, S.; Palitti, F.; Kaul, S.; Boehm, T.L.J.; Hirth, H.P.; Drahovsky, D.
Purification and characterization of mammalian DNA methyltransferases by use of monoclonal antibodies
J. Biol. Chem.
260
13787-13793
1985
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Pfeifer, G.P.; Grunwald, S.; Boehm, T.L.J.; Drahovsky, D.
Isolation and characterization of DNA cytosine 5-methyltransferase from human placenta
Biochim. Biophys. Acta
740
323-330
1983
Homo sapiens
Manually annotated by BRENDA team
McIntosh, L.P.; Zielinski, W.S.; Kalisch, B.W.; Pfeifer, G.P.; Sprinzl, M.; Drahovsky, D.; van de Sande, J.H.; Jovin, T.M.
Synthesis and characterization of poly[d(G-z5C)]. B-Z transition and inhibition of DNA methylase
Biochemistry
24
4806-4814
1985
Homo sapiens
Manually annotated by BRENDA team
Laayoun, A.; Smith, S.S.
Methylation of slipped duplexes, snapbacks and cruciforms by human DNA(cytosine-5)methyltransferase
Nucleic Acids Res.
23
1584-1589
1995
Homo sapiens
Manually annotated by BRENDA team
Pradhan, S.; Bacolla, A.; Wells, R.D.; Roberts, R.J.
Recombinant human DNA (cytosine-5) methyltransferase. I. Expression, purification, and comparison of DE Novo and maintenance methylation
J. Biol. Chem.
274
33002-33010
1999
Homo sapiens
Manually annotated by BRENDA team
Bacolla, A.; Pradhan, S.; Larson, J.E.; Roberts, R.J.; Wells, R.D.
Recombinant human DNA (cytosine-5) methyltransferase: III. Allosteric control, reaction order, and influence of plasmid topology and triplet repeat length on methylation of the fragile X CGG*CCG sequence
J. Biol. Chem.
276
18605-18613
2001
Homo sapiens (P26358), Homo sapiens
Manually annotated by BRENDA team
Siedlecki, P.; Boy, R.G.; Comagic, S.; Schirrmacher, R.; Wiessler, M.; Zielenkiewicz, P.; Suhai, S.; Lyko, F.
Establishment and functional validation of a structural homology model for human DNA methyltransferase 1
Biochem. Biophys. Res. Commun.
306
558-563
2003
Homo sapiens (P26358), Homo sapiens
Manually annotated by BRENDA team
Robertson, A.K.; Geiman, T.M.; Sankpal, U.T.; Hager, G.L.; Robertson, K.D.
Effects of chromatin structure on the enzymatic and DNA binding functions of DNA methyltransferases DNMT1 and Dnmt3a in vitro
Biochem. Biophys. Res. Commun.
322
110-118
2004
Homo sapiens (P26358), Homo sapiens (Q9Y6K1)
Manually annotated by BRENDA team
Pradhan, S.; Esteve, P.O.
Allosteric activator domain of maintenance human DNA (cytosine-5) methyltransferase and its role in methylation spreading
Biochemistry
42
5321-5332
2003
Homo sapiens
Manually annotated by BRENDA team
Yokochi, T.; Robertson, K.D.
Dimethyl sulfoxide stimulates the catalytic activity of de novo DNA methyltransferase 3a (Dnmt3a) in vitro
Bioorg. Chem.
32
234-243
2004
Homo sapiens (Q9UBC3)
Manually annotated by BRENDA team
Fang, M.Z.; Wang, Y.; Ai, N.; Hou, Z.; Sun, Y.; Lu, H.; Welsh, W.; Yang, C.S.
Tea polyphenol (-)-epigallocatechin-3-gallate inhibits DNA methyltransferase and reactivates methylation-silenced genes in cancer cell lines
Cancer Res.
63
7563-7570
2003
Homo sapiens
Manually annotated by BRENDA team
Hermann, A.; Schmitt, S.; Jeltsch, A.
The human Dnmt2 has residual DNA-(cytosine-C5) methyltransferase activity
J. Biol. Chem.
278
31717-31721
2003
Homo sapiens
Manually annotated by BRENDA team
Casillas, M.A.; Lopatina, N.; Andrews, L.G.; Tollefsbol, T.O.
Transcriptional control of the DNA methyltransferases is altered in aging and neoplastically-transformed human fibroblasts
Mol. Cell. Biochem.
252
33-43
2003
Homo sapiens (P26358), Homo sapiens (Q9UBC3), Homo sapiens (Q9Y6K1), Homo sapiens
Manually annotated by BRENDA team
Geiman, T.M.; Sankpal, U.T.; Robertson, A.K.; Chen, Y.; Mazumdar, M.; Heale, J.T.; Schmiesing, J.A.; Kim, W.; Yokomori, K.; Zhao, Y.; Robertson, K.D.
Isolation and characterization of a novel DNA methyltransferase complex linking DNMT3B with components of the mitotic chromosome condensation machinery
Nucleic Acids Res.
32
2716-2729
2004
Homo sapiens (Q9UBC3)
Manually annotated by BRENDA team
Hodge, D.R.; Cho, E.; Copeland, T.D.; Guszczynski, T.; Yang, E.; Seth, A.K.; Farrar, W.L.
IL-6 enhances the nuclear translocation of DNA cytosine-5-methyltransferase 1 (DNMT1) via phosphorylation of the nuclear localization sequence by the AKT kinase
Cancer Genomics Proteomics
4
387-398
2008
Homo sapiens
Manually annotated by BRENDA team
Guo, Z.; Tsai, M.H.; Shiao, Y.H.; Chen, L.H.; Wei, M.L.; Lv, X.; Gius, D.; Little, J.B.; Mitchell, J.B.; Chuang, E.Y.
DNA (cytosine-5)-methyltransferase 1 as a mediator of mutant p53-determined p16(ink4A) down-regulation
J. Biomed. Sci.
15
163-168
2008
Homo sapiens
Manually annotated by BRENDA team
Pradhan, M.; Esteve, P.O.; Chin, H.G.; Samaranayke, M.; Kim, G.D.; Pradhan, S.
CXXC domain of human DNMT1 is essential for enzymatic activity
Biochemistry
47
10000-10009
2008
Homo sapiens (P26358), Homo sapiens
Manually annotated by BRENDA team
Wang, H.; Wang, Y.
6-Thioguanine perturbs cytosine methylation at the CpG dinucleotide site by DNA methyltransferases in vitro and acts as a DNA demethylating agent in vivo
Biochemistry
48
2290-2299
2009
Haemophilus parainfluenzae, Homo sapiens
Manually annotated by BRENDA team
Saavedra, O.M.; Isakovic, L.; Llewellyn, D.B.; Zhan, L.; Bernstein, N.; Claridge, S.; Raeppel, F.; Vaisburg, A.; Elowe, N.; Petschner, A.J.; Rahil, J.; Beaulieu, N.; MacLeod, A.R.; Delorme, D.; Besterman, J.M.; Wahhab, A.
SAR around (L)-S-adenosyl-L-homocysteine, an inhibitor of human DNA methyltransferase (DNMT) enzymes
Bioorg. Med. Chem. Lett.
19
2747-2751
2009
Homo sapiens (P26358), Homo sapiens (Q9UBC3), Homo sapiens
Manually annotated by BRENDA team
Kuck, D.; Singh, N.; Lyko, F.; Medina-Franco, J.L.
Novel and selective DNA methyltransferase inhibitors: docking-based virtual screening and experimental evaluation
Bioorg. Med. Chem.
18
822-829
2009
Homo sapiens
Manually annotated by BRENDA team
Ooi, S.K.; Bestor, T.H.
The colorful history of active DNA demethylation
Cell
133
1145-1148
2008
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Singh, N.; Duenas-Gonzalez, A.; Lyko, F.; Medina-Franco, J.L.
Molecular modeling and molecular dynamics studies of hydralazine with human DNA methyltransferase 1
ChemMedChem
4
792-799
2009
Homo sapiens
Manually annotated by BRENDA team
Svedruzic, Z.M.
Mammalian cytosine DNA methyltransferase Dnmt1: enzymatic mechanism, novel mechanism-based inhibitors, and RNA-directed DNA methylation
Curr. Med. Chem.
15
92-106
2008
Homo sapiens (P26358)
Manually annotated by BRENDA team
Balada, E.; Ordi-Ros, J.; Serrano-Acedo, S.; Martinez-Lostao, L.; Rosa-Leyva, M.; Vilardell-Tarres, M.
Transcript levels of DNA methyltransferases DNMT1, DNMT3A and DNMT3B in CD4+ T cells from patients with systemic lupus erythematosus
Immunology
124
339-347
2008
Homo sapiens
Manually annotated by BRENDA team
Cavalieri Gomes, C.; da Silveira e Oliveira, C.; Santos Pimenta, L.G.; De Marco, L.; Santiago Gomez, R.
Immunolocalization of DNMT1 and DNMT3a in salivary gland neoplasms
Pathobiology
76
136-140
2009
Homo sapiens
Manually annotated by BRENDA team
Ye, Y.; Stivers, J.T.
Fluorescence-based high-throughput assay for human DNA (cytosine-5)-methyltransferase 1
Anal. Biochem.
401
168-172
2010
Homo sapiens
Manually annotated by BRENDA team
Ye, F.; Xu, X.C.
Benzo[a]pyrene diol epoxide suppresses retinoic acid receptor-beta2 expression by recruiting DNA (cytosine-5-)-methyltransferase 3A
Mol. Cancer
9
93
2010
Homo sapiens
Manually annotated by BRENDA team
Zhang, G.; Esteve, P.; Chin, H.; Terragni, J.; Dai, N.; Correa, I.J.; Pradhan, S.
Small RNA-mediated DNA (cytosine-5) methyltransferase 1 inhibition leads to aberrant DNA methylation
Nucleic Acids Res.
43
6112-6124
2015
Homo sapiens (P26358)
Manually annotated by BRENDA team