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S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
additional information
?
-
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
-
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position
-
-
?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position
-
-
?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position
-
-
?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
-
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position
-
-
?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position
-
-
?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
-
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G
-
-
?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G
-
-
?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G
-
-
?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
-
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G
-
-
?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
in vitro, the tRNA ribose 2'-O-methyltransferase aTrm56 specifically catalyses the AdoMet-dependent 2'-O-ribose methylation of cytidine position 56 in tRNA transcripts. In vitro transcribed Escherichia coli tRNALeu2, yeast tRNAPhe, and Pyrococcus abyssi tRNALeu(CAa) tRNAs are methylated with significant initial rates. Mutations of cytosine at position 56 either by uridine or guanine in tRNA abolish methyl incorporation. A tRNA minisubstrate corresponding to the yeast tRNAAsp transcript missing the D stem-loop, is methylated in vitro at a slightly lower efficiency than the wild-type tRNAAsp. A minisubstrate consisting of the T-loop of tRNAAsp closed by a synthetic stem is also tested. This TSL minisubstrate is still methylated in vitro even though the reaction is 4-5fold less efficient than with the wild type substrate. The complete 3D architecture of the tRNA substrate is not required for aTrm56 catalysis. A T stem-loop structure alone is thus a minimal substrate recognized by aTrm56
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
Pyrococcus horikoshii tRNAVal. aTrm56 prefers the L-shaped tRNA to the lambda form as its substrate
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
-
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
the methylated nucleotide is identified as pCm
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
the methylated nucleotide is identified as pCm
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
the methylated nucleotide is identified as pCm
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
-
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
the methylated nucleotide is identified as pCm
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
the methylated nucleotide is identified as pCm
-
-
?
additional information
?
-
purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed
-
-
-
additional information
?
-
-
purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed
-
-
-
additional information
?
-
purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed
-
-
-
additional information
?
-
purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed
-
-
-
additional information
?
-
purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed
-
-
-
additional information
?
-
purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
additional information
?
-
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position
-
-
?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position
-
-
?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position
-
-
?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position
-
-
?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position
-
-
?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G
-
-
?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G
-
-
?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G
-
-
?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G
-
-
?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
the methylated nucleotide is identified as pCm
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
the methylated nucleotide is identified as pCm
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
the methylated nucleotide is identified as pCm
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
the methylated nucleotide is identified as pCm
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
the methylated nucleotide is identified as pCm
-
-
?
additional information
?
-
purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed
-
-
-
additional information
?
-
-
purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed
-
-
-
additional information
?
-
purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed
-
-
-
additional information
?
-
purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed
-
-
-
additional information
?
-
purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed
-
-
-
additional information
?
-
purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
physiological function
the enzyme Trm56 is responsible for the typical archaeal tRNA modification of 2'-O-methylcytidine at position 56 (Cm56). The Cm modification is only found at position 56 in native tRNALeuUAG. Trm56 methylates both the elongator tRNAMet transcript and its precursor. But the methyl group acceptance activity of the precursor is considerably lower than that of the mature transcript, the methylation by Trm56 occurs mainly after the removal of the intron
physiological function
-
the enzyme Trm56 is responsible for the typical archaeal tRNA modification of 2'-O-methylcytidine at position 56 (Cm56). The Cm modification is only found at position 56 in native tRNALeuUAG. Trm56 methylates both the elongator tRNAMet transcript and its precursor. But the methyl group acceptance activity of the precursor is considerably lower than that of the mature transcript, the methylation by Trm56 occurs mainly after the removal of the intron
-
physiological function
-
the enzyme Trm56 is responsible for the typical archaeal tRNA modification of 2'-O-methylcytidine at position 56 (Cm56). The Cm modification is only found at position 56 in native tRNALeuUAG. Trm56 methylates both the elongator tRNAMet transcript and its precursor. But the methyl group acceptance activity of the precursor is considerably lower than that of the mature transcript, the methylation by Trm56 occurs mainly after the removal of the intron
-
physiological function
-
the enzyme Trm56 is responsible for the typical archaeal tRNA modification of 2'-O-methylcytidine at position 56 (Cm56). The Cm modification is only found at position 56 in native tRNALeuUAG. Trm56 methylates both the elongator tRNAMet transcript and its precursor. But the methyl group acceptance activity of the precursor is considerably lower than that of the mature transcript, the methylation by Trm56 occurs mainly after the removal of the intron
-
physiological function
-
the enzyme Trm56 is responsible for the typical archaeal tRNA modification of 2'-O-methylcytidine at position 56 (Cm56). The Cm modification is only found at position 56 in native tRNALeuUAG. Trm56 methylates both the elongator tRNAMet transcript and its precursor. But the methyl group acceptance activity of the precursor is considerably lower than that of the mature transcript, the methylation by Trm56 occurs mainly after the removal of the intron
-
additional information
Trm56 and an yet unknown tRNA (m7G49) methyltransferase might be included in protein complexes and precipitated by centrifugation at 30000 × g. Thermoplasma acidophilum Trm56 might be part of a large complex with other proteins in living cells
additional information
-
Trm56 and an yet unknown tRNA (m7G49) methyltransferase might be included in protein complexes and precipitated by centrifugation at 30000 × g. Thermoplasma acidophilum Trm56 might be part of a large complex with other proteins in living cells
additional information
-
Trm56 and an yet unknown tRNA (m7G49) methyltransferase might be included in protein complexes and precipitated by centrifugation at 30000 × g. Thermoplasma acidophilum Trm56 might be part of a large complex with other proteins in living cells
-
additional information
-
Trm56 and an yet unknown tRNA (m7G49) methyltransferase might be included in protein complexes and precipitated by centrifugation at 30000 × g. Thermoplasma acidophilum Trm56 might be part of a large complex with other proteins in living cells
-
additional information
-
Trm56 and an yet unknown tRNA (m7G49) methyltransferase might be included in protein complexes and precipitated by centrifugation at 30000 × g. Thermoplasma acidophilum Trm56 might be part of a large complex with other proteins in living cells
-
additional information
-
Trm56 and an yet unknown tRNA (m7G49) methyltransferase might be included in protein complexes and precipitated by centrifugation at 30000 × g. Thermoplasma acidophilum Trm56 might be part of a large complex with other proteins in living cells
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Kuratani, M.; Bessho, Y.; Nishimoto, M.; Grosjean, H.; Yokoyama, S.
Crystal structure and mutational study of a unique SpoU family archaeal methylase that forms 2'-O-methylcytidine at position 56 of tRNA
J. Mol. Biol.
375
1064-1075
2008
Pyrococcus horikoshii (O58214), Pyrococcus horikoshii
brenda
Renalier, M.H.; Joseph, N.; Gaspin, C.; Thebault, P.; Mougin, A.
The Cm56 tRNA modification in archaea is catalyzed either by a specific 2'-O-methylase, or a C/D sRNP
RNA
11
1051-1063
2005
Pyrococcus abyssi (Q9UYD1), Pyrococcus abyssi
brenda
Kawamura, T.; Anraku, R.; Hasegawa, T.; Tomikawa, C.; Hori, H.
Transfer RNA methyltransferases from Thermoplasma acidophilum, a thermoacidophilic archaeon
Int. J. Mol. Sci.
16
91-113
2014
Thermoplasma acidophilum, Thermoplasma acidophilum HO-62
brenda
Kawamura, T.; Anraku, R.; Hasegawa, T.; Tomikawa, C.; Hori, H.
Transfer RNA methyltransferases from Thermoplasma acidophilum, a thermoacidophilic archaeon
Int. J. Mol. Sci.
16
91-113
2015
Thermoplasma acidophilum (Q9HJN6), Thermoplasma acidophilum, Thermoplasma acidophilum JCM 9062 (Q9HJN6), Thermoplasma acidophilum AMRC-C165 (Q9HJN6), Thermoplasma acidophilum ATCC 25905 (Q9HJN6), Thermoplasma acidophilum NBRC 15155 (Q9HJN6)
brenda