Information on EC 1.8.4.11 - peptide-methionine (S)-S-oxide reductase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Search Reference ID:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
1.8.4.11
-
RECOMMENDED NAME
GeneOntology No.
peptide-methionine (S)-S-oxide reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
L-methionine + thioredoxin disulfide + H2O = L-methionine (S)-S-oxide + thioredoxin
show the reaction diagram
peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
peptide-L-methionine:thioredoxin-disulfide S-oxidoreductase [L-methionine (S)-S-oxide-forming]
The reaction occurs in the reverse direction to that shown above. The enzyme exhibits high specificity for the reduction of the S-form of L-methionine S-oxide, acting faster on the residue in a peptide than on the free amino acid [9]. On the free amino acid, it can also reduce D-methionine (S)-S-oxide but more slowly [9]. The enzyme plays a role in preventing oxidative-stress damage caused by reactive oxygen species by reducing the oxidized form of methionine back to methionine and thereby reactivating peptides that had been damaged. In some species, e.g. Neisseria meningitidis, both this enzyme and EC 1.8.4.12, peptide-methionine (R)-S-oxide reductase, are found within the same protein whereas, in other species, they are separate proteins [1,4]. The reaction proceeds via a sulfenic-acid intermediate [5,10].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain N2
-
-
Manually annotated by BRENDA team
strain NCTC 11168
-
-
Manually annotated by BRENDA team
strain NCTC 11168
-
-
Manually annotated by BRENDA team
strain OhILAs, also known as Alkaliphilus oremlandii
-
-
Manually annotated by BRENDA team
strain OhILAs, also known as Alkaliphilus oremlandii
-
-
Manually annotated by BRENDA team
strain E88
-
-
Manually annotated by BRENDA team
strain E88
-
-
Manually annotated by BRENDA team
strain Z19
-
-
Manually annotated by BRENDA team
; gene msr encodes an enzyme showing both MsrA, methionine S-oxide reductase (S-form oxidizing), and MsrB, methionine S-oxide reductase (R-form oxidizing), activity
-
-
Manually annotated by BRENDA team
ATCC 43049
-
-
Manually annotated by BRENDA team
strain NRC-1
-
-
Manually annotated by BRENDA team
strain NRC-1
-
-
Manually annotated by BRENDA team
strain 26695, enzyme forms MsrA and MsrB are fused together forming a single enzyme termed Msr
-
-
Manually annotated by BRENDA team
gene msrA, isozymes MsrA1-3
-
-
Manually annotated by BRENDA team
DSM 6242
-
-
Manually annotated by BRENDA team
strain S2
-
-
Manually annotated by BRENDA team
strain C2A
-
-
Manually annotated by BRENDA team
strain Fusaro
-
-
Manually annotated by BRENDA team
strain Fusaro
-
-
Manually annotated by BRENDA team
strain Go1
-
-
Manually annotated by BRENDA team
Methanothermobacter thermautotrophicum
strain delta H
-
-
Manually annotated by BRENDA team
DSM 2160
-
-
Manually annotated by BRENDA team
strain MS11, variant VD300, bifunctional enzyme MsrA/B, gene pilA
-
-
Manually annotated by BRENDA team
strain MS11A derivatives, bifunctional enzyme MsrA/B, gene pilB
-
-
Manually annotated by BRENDA team
cultivar Hacana SRI
-
-
Manually annotated by BRENDA team
no activity in Aeropyrum pernix K1
-
-
-
Manually annotated by BRENDA team
no activity in Aquifex aeolicus
-
-
-
Manually annotated by BRENDA team
no activity in Archaeoglobus fulgidus DSM 4304
-
-
-
Manually annotated by BRENDA team
no activity in Bifidobacterium longum
NCC2705
-
-
Manually annotated by BRENDA team
no activity in Bifidobacterium longum NCC2705
NCC2705
-
-
Manually annotated by BRENDA team
no activity in Methanocaldococcus jannaschii
DSM 2661
-
-
Manually annotated by BRENDA team
no activity in Methanopyrus kandleri
strain AV19
-
-
Manually annotated by BRENDA team
no activity in Methanopyrus kandleri AV19
strain AV19
-
-
Manually annotated by BRENDA team
no activity in Nanoarchaeum equitans
Kin4-M
-
-
Manually annotated by BRENDA team
no activity in Nanoarchaeum equitans Kin4-M
Kin4-M
-
-
Manually annotated by BRENDA team
no activity in Pyrobaculum aerophilum
strain IM2
-
-
Manually annotated by BRENDA team
no activity in Pyrobaculum aerophilum IM2
strain IM2
-
-
Manually annotated by BRENDA team
no activity in Pyrococcus abyssi
strain GE5
-
-
Manually annotated by BRENDA team
no activity in Pyrococcus abyssi GE5
strain GE5
-
-
Manually annotated by BRENDA team
no activity in Pyrococcus furiosus
strain DSM 3638
-
-
Manually annotated by BRENDA team
no activity in Pyrococcus horikoshii
strain OT3
-
-
Manually annotated by BRENDA team
no activity in Pyrococcus horikoshii OT3
strain OT3
-
-
Manually annotated by BRENDA team
no activity in Sulfolobus tokodaii
strain 7
-
-
Manually annotated by BRENDA team
no activity in Sulfolobus tokodaii 7
strain 7
-
-
Manually annotated by BRENDA team
no activity in Thermoplasma acidophilum
DSM 1728
-
-
Manually annotated by BRENDA team
no activity in Thermoplasma volcanium
strain GSS1
-
-
Manually annotated by BRENDA team
no activity in Thermoplasma volcanium GSS1
strain GSS1
-
-
Manually annotated by BRENDA team
no activity in Thermotoga maritima
-
-
-
Manually annotated by BRENDA team
strain DSM 9790
-
-
Manually annotated by BRENDA team
strain BY4743, gene msrA
-
-
Manually annotated by BRENDA team
winter rye, cv. Halo
-
-
Manually annotated by BRENDA team
3 copies of gene msrA
-
-
Manually annotated by BRENDA team
; gene msr encodes an enzyme showing both MsrA, methionine S-oxide reductase (S-form oxidizing), and MsrB, methionine S-oxide reductase (R-form oxidizing), activity
-
-
Manually annotated by BRENDA team
strain R6x, gene msrA
-
-
Manually annotated by BRENDA team
DSM 639
-
-
Manually annotated by BRENDA team
strain P2
-
-
Manually annotated by BRENDA team
strain P2
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Trypanosoma sp. CL-Brener
-
-
-
Manually annotated by BRENDA team
2 isozymes of MsrA
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
enzyme-deficient mice are more susceptible to kidney ischemia/reperfusion injury than wild type mice. Deletion of the enzyme enhances renal functional and morphological impairments, congestion, inflammatory responses, and oxidative stress under ischemia/reperfusion conditions
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-methyl 4-tolyl sulfoxide + thioredoxin
?
show the reaction diagram
acetyl-L-methionine-(S)-S-oxide-NHMe + thioredoxin
?
show the reaction diagram
-
-
-
-
?
alpha-synuclein + dithiothreitol
?
show the reaction diagram
-
alpha-synuclein is oxidized at both Met1 and Met5 but not at Met116 or Met127
-
-
?
alpha-synuclein + thioredoxin disulfide + H2O
?
show the reaction diagram
-
Met1 and Met5 within alpha-synuclein are oxidized to (S)-methionine sulfoxide
-
-
?
alpha-synuclein-L-methionine (S)-S-oxide + thioredoxin
alpha-synuclein-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
-
-
-
?
alpha1-antitrypsin + thioredoxin disulfide + H2O
?
show the reaction diagram
-
Met358 within alpha1-antitrypsin is oxidized to (S)-methionine sulfoxide
-
-
?
apolipoprotein A-I + dithiothreitol
?
show the reaction diagram
-
the myristoylated enzyme reduces the methionine sulfoxides in apolipoprotein A-I four times faster than nonmyristoylated enzyme
-
-
?
calmodulin + thioredoxin disulfide + H2O
?
show the reaction diagram
-
Met77 within calmodulin is oxidized to (S)-methionine sulfoxide
-
-
r
calmodulin L-methionine-(S)-sulfoxide + thioredoxin
calmodulin L-methionine + thioredoxin disulfide
show the reaction diagram
calmodulin-L-methionine (S)-S-oxide + thioredoxin
calmodulin-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
-
MsrBA is able to completely reduce (i.e., repair) MetSO in the calcium regulatory protein calmodulin. The efficient repair is the coordinate activity of the two catalytic domains in the MsrBA fusion protein, which results in a 1 order of magnitude rate enhancement in comparison to those of the individual MsrA or MsrB enzyme alone
-
-
?
calmodulin-L-methionine (S)-sulfoxide + thioredoxin
calmodulin-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
-
-
-
-
?
dabsyl-L-methionine (R)-sulfoxide + thioredoxin
dabsyl-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
-
-
-
?
dabsyl-L-methionine (S)-sulfoxide + dithiohtreitol
dabsyl-L-methionine + dithiothreitol disulfide + H2O
show the reaction diagram
-
-
-
?
dabsyl-L-methionine (S)-sulfoxide + dithiothreitol
dabsyl-L-methionine + dithiothreitol disulfide
show the reaction diagram
dabsyl-L-methionine (S)-sulfoxide + dithiothreitol
dabsyl-L-methionine + dithiothreitol disulfide + H2O
show the reaction diagram
dabsyl-L-methionine (S)-sulfoxide + NADPH + H+
dabsyl-L-methionine + NADP+ + H2O
show the reaction diagram
synthetic substrate, MsrA is absolutely specific for the S-form, 7fold lower activity with NADPH compared to DTT
-
-
?
dabsyl-L-methionine (S)-sulfoxide + thioredoxin
?
show the reaction diagram
dabsyl-L-methionine (S)-sulfoxide + thioredoxin
dabsyl-L-methionine + thioredoxin disulfide
show the reaction diagram
dabsyl-L-methionine (S)-sulfoxide + thioredoxin
dabsyl-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
dabsyl-L-methionine-(S)-S-oxide + dithiothreitol
dabsyl-L-methionine + dithiothreitol disulfide + H2O
show the reaction diagram
-
-
-
-
?
dabsyl-L-methionine-(S)-S-oxide + dithiothreitol
dabsyl-L-methionine + DTT disulfide + H2O
show the reaction diagram
-
-
-
?
dabsyl-L-methionine-(S)-S-oxide + DTT
dabsyl-L-methionine + DTT disulfide + H2O
show the reaction diagram
dabsyl-L-methionine-(S)-S-oxide + thioredoxin
dabsyl-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
dabsyl-L-methionine-(S)-S-sulfoxide + dithiothreitol
dabsyl-L-methionine + dithiothreitol disulfide + H2O
show the reaction diagram
DL-methionine (S)-sulfoxide + thioredoxin
DL-methionine + thioredoxin disulfide + H2O
show the reaction diagram
-
enzyme MsrA is specific for the S-form, active on free and protein-bound methionine, the latter is bound more efficiently
-
-
?
Fmoc-L-methionine (S)-sulfoxide + dithiothreitol
Fmoc-L-methionine + dithiothreitol disulfide + H2O
show the reaction diagram
Gly-L-Met-Gly + dithiothreitol
?
show the reaction diagram
-
-
-
-
?
His6-Ala-Ala-Gln-MetO-Ile + DTT
His6-Ala-Ala-Gln-Met-Ile + DTT disulfide + H2O
show the reaction diagram
-
-
-
-
?
Hsp21 L-methionine S-oxide + dithiothreitol
Hsp21 L-methionine + dithiothreitol S-oxide
show the reaction diagram
L-methionine (R)-sulfoxide + thioredoxin
L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
-
the membrane-associated isozyme reduces both R- and S-stereoisomers of methionine sulfoxide in proteins
-
-
?
L-methionine (R,S)-sulfoxide + glutathione
L-methionine + GSSG + H2O
show the reaction diagram
-
-
-
-
?
L-methionine (R,S)-sulfoxide + thioredoxin
L-methionine + thioredoxin disulfide
show the reaction diagram
L-methionine (S)-sulfoxide + 2 dithiothreitol
L-methionine + dithiothreitol disulfide + H2O
show the reaction diagram
L-methionine (S)-sulfoxide + dithiothreitol
?
show the reaction diagram
-
the myristoylated enzyme form reduces methionine sulfoxide in protein much faster than the nonmyristoylated form
-
-
?
L-methionine (S)-sulfoxide + thioredoxin
L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
L-methionine (S)-sulfoxide + tryparedoxin I
L-methionine + tryparedoxin I disulfide + H2O
show the reaction diagram
L-methionine sulfoxide enkephalin + thioredoxin
L-methionine enkephalin
show the reaction diagram
-
membrane-bound enzyme form Mem-R,S-Msr
-
-
?
L-methionine-(S)-S-oxide + DTT
L-methionine + DTT disulfide + H2O
show the reaction diagram
stereospecific reduction, 9-fluorenylmethyl chloroformate-labeled substrate
-
-
?
L-methionine-(S)-S-oxide + thioredoxin
L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
L-Pro-L-Met-L-Ala-L-Ile-L-Lys-L-Lys + dithiothreitol
?
show the reaction diagram
-
-
-
-
?
N-acetyl-L-methionine (R)-sulfoxide + thioredoxin
N-acetyl-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
-
the membrane-associated isozyme reduces both R- and S-stereoisomer of methionine sulfoxide in proteins
-
-
?
N-acetyl-L-methionine (R,S)-sulfoxide + thioredoxin
N-acetyl-L-methionine + thioredoxin disulfide
show the reaction diagram
N-acetyl-L-methionine (S)-sulfoxide + thioredoxin
N-acetyl-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
N-acetyl-L-methionine (S)-sulfoxide methyl ester + thioredoxin
N-acetyl-L-methionine methyl ester + thioredoxin disulfide + H2O
show the reaction diagram
-
enzyme MsrA
-
-
?
N-acetyl-L-methionine-(S)-S-oxide + DTT
N-acetyl-L-methionine + DTT disulfide + H2O
show the reaction diagram
N-acetyl-L-methionine-(S)-S-oxide + thioredoxin
N-acetyl-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
oxidized calmodulin + thioredoxin
partially reduced calmodulin + thioredoxin disulfide
show the reaction diagram
-
enzyme reduces L-methionine (S)-sulfoxide of the protein substrate
-
-
?
peptide-L-methionine (S)-S-oxide + thioredoxin
peptide-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
MsrA suppresses dopaminergic cell death and protein aggregation induced by the complex I inhibitor rotenone or mutant alpha-synuclein, but not by the proteasome inhibitor MG132. MsrA protects against Parkinson's disease-related stresses primarily via methionine sulfoxide repair rather than by scavenging reactive oxygen species
-
-
?
peptide-L-methionine-(S)-S-oxide + DTT
peptide-L-methionine + DTT disulfide + H2O
show the reaction diagram
peptide-L-methionine-(S)-S-oxide + thioredoxin
peptide-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
protein-L-methionine (S)-S-oxide + thioredoxin
protein-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
-
Met sulfoxide residues in Met-rich proteins can be reduced by MsrA and MsrB
-
-
?
protein-L-methionine (S)-sulfoxide + thioredoxin
protein-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
ribosomal protein L12-L-methionine (S)-sulfoxide + thioredoxin
ribosomal protein L12-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
-
-
-
-
?
sulindac + thioredoxin
sulindac sulfide + thioredoxin disulfide
show the reaction diagram
sulindac + thioredoxin
sulindac sulfide + thioredoxin disulfide + H2O
show the reaction diagram
Tyr-Gly-Gly-Phe-L-methionine-(S)-S-oxide + DTT
Tyr-Gly-Gly-Phe-L-methionine + DTT disulfide + H2O
show the reaction diagram
Tyr-Gly-Gly-Phe-L-methionine-(S)-S-oxide + thioredoxin
Tyr-Gly-Gly-Phe-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
calmodulin L-methionine-(S)-sulfoxide + thioredoxin
calmodulin L-methionine + thioredoxin disulfide
show the reaction diagram
-
MsrA is specific for the S-form, enzyme provides protection against oxidative damage by reactive oxygen species and has a repair function for oxidized protein methionine residues, which restores the calmodulin binding to adenylate cyclase of the pathogen Bordetella pertussis, which is an essential step for the bacterium to enter host cells, overview
-
-
?
calmodulin-L-methionine (S)-sulfoxide + thioredoxin
calmodulin-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
-
-
-
-
?
dabsyl-L-methionine (S)-sulfoxide + thioredoxin
dabsyl-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
-
-
-
-
?
Hsp21 L-methionine S-oxide + dithiothreitol
Hsp21 L-methionine + dithiothreitol S-oxide
show the reaction diagram
-
chloroplast-localized small heat shock protein, repair function for heat shock protein Hsp21 by restoring the structure, which is crucial for cellular resistance to oxidative stress, the enzyme can protect the chaperone-like activity of Hsp21
-
-
?
L-methionine (R,S)-sulfoxide + thioredoxin
L-methionine + thioredoxin disulfide
show the reaction diagram
L-methionine (S)-sulfoxide + 2 dithiothreitol
L-methionine + dithiothreitol disulfide + H2O
show the reaction diagram
L-methionine (S)-sulfoxide + dithiothreitol
?
show the reaction diagram
-
the myristoylated enzyme form reduces methionine sulfoxide in protein much faster than the nonmyristoylated form
-
-
?
L-methionine (S)-sulfoxide + thioredoxin
L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
L-methionine-(S)-S-oxide + thioredoxin
L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
peptide-L-methionine (S)-S-oxide + thioredoxin
peptide-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
P54149
MsrA suppresses dopaminergic cell death and protein aggregation induced by the complex I inhibitor rotenone or mutant alpha-synuclein, but not by the proteasome inhibitor MG132. MsrA protects against Parkinson's disease-related stresses primarily via methionine sulfoxide repair rather than by scavenging reactive oxygen species
-
-
?
peptide-L-methionine-(S)-S-oxide + thioredoxin
peptide-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
protein-L-methionine (S)-S-oxide + thioredoxin
protein-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
-
Met sulfoxide residues in Met-rich proteins can be reduced by MsrA and MsrB
-
-
?
protein-L-methionine (S)-sulfoxide + thioredoxin
protein-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
ribosomal protein L12-L-methionine (S)-sulfoxide + thioredoxin
ribosomal protein L12-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
-
-
-
-
?
sulindac + thioredoxin
sulindac sulfide + thioredoxin disulfide
show the reaction diagram
sulindac + thioredoxin
sulindac sulfide + thioredoxin disulfide + H2O
show the reaction diagram
Tyr-Gly-Gly-Phe-L-methionine-(S)-S-oxide + thioredoxin
Tyr-Gly-Gly-Phe-L-methionine + thioredoxin disulfide + H2O
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM