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(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide + 2 NADPH + 2 H+
(2E)-2-[[5-(hydroxyamino)furan-2-yl]methylidene]hydrazine-1-carboxamide + 2 NADP+
1,3-dinitrobenzene + 2 NADPH + 2 H+
1-amino-3-nitrobenzene + 2 NADP+ + H2O
-
-
about 90% 1-amino-3-nitrobenzene plus 10% 1-hydroxylamino-3-nitrobenzene
-
?
1,3-dinitrobenzene + 2 NADPH + 2 H+
? + 2 NADP+
1-hydroxylamino-3-nitrobenzene + 2 NADPH + 2 H+
1-amino-3-nitrobenzene + 2 NADP+ + H2O
-
-
-
-
?
1-nitroso-3-nitrobenzene + 2 NADPH + 2 H+
? + 2 NADP+ + H2O
-
-
only the amino product can be observed
-
?
2,4,6-trinitrotoluene + 2 NADPH + 2 H+
? + 2 NADP+ + H2O
-
-
products are hydroxylamino- and amino-dinitrotoluene isomersd
-
?
2,4-dichloro-1-nitrobenzene + 2 NADPH + 2 H+
2,4-dichloro-N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
-
?
2,4-dinitrochlorobenzene + 2 NADPH + 2 H+
? + 2 NADP+ + H2O
2,4-dinitrotoluene + 2 NADPH + 2 H+
? + 2 NADP+ + H2O
2-chloro-6-nitrotoluene + 3 NADPH + 3 H+
3-chloro-2-methylaniline + 3 NADP+ + 2 H2O
-
-
-
-
?
2-chloronitrobenzene + 2 NADPH + 2 H+
2-chloro-N-hydroxyaniline + 2 NADP+ + H2O
2-nitrotoluene + 3 NADPH + 3 H+
2-toluidine + 3 NADP+ + 2 H2O
-
-
-
-
?
3-bromo-4-nitrotoluene + 3 NADPH + 3 H+
2-bromo-4-methylaniline + 3 NADP+ + 2 H2O
-
-
-
-
?
3-chloronitrobenzene + 2 NADPH + 2 H+
3-chloro-N-hydroxyaniline + 2 NADP+ + H2O
3-nitrophenol + 2 NADPH + 2 H+
3-(hydroxyamino)phenol + 2 NADP+ + H2O
-
recombinant protein, 66% of the activity with 4-chloronitrobenzene
-
?
3-nitrotoluene + 2 NADPH + 2 H+
N-hydroxy-3-methylaniline + 2 NADP+ + H2O
4-chloronitrobenzene + 2 NADH + 2 H+
4-chloro-N-hydroxyaniline + 2 NAD+ + H2O
-
-
-
?
4-chloronitrobenzene + 2 NADPH + 2 H+
2-amino-5-chlorophenol + 2 NADP+ + H2O
-
reaction in presence of hydroxylaminobenzene mutase CnbB
-
?
4-chloronitrobenzene + 2 NADPH + 2 H+
4-chloro-N-hydroxyaniline + 2 NADP+ + H2O
4-nitrochlorobenzene + 3 NADPH + 3 H+
4-chloroaniline + 3 NADP+ + 2 H2O
-
-
-
-
?
4-nitrophenol + 3 NADPH + 3 H+
4-aminophenol + 3 NADP+ + 2 H2O
4-nitrotoluene + 2 NADPH + 2 H+
N-hydroxy-4-methylaniline + 2 NADP+ + H2O
-
-
-
?
5-(aziridin-1-yl)-2,4-dinitrobenzamide + 2 NADPH + 2 H+
? + 2 NADP+
-
enzyme activates the dinitrobenzamide cancer prodrug CB1954, i.e. 5-(aziridin-1-yl)-2,4-dinitrobenzamide
-
?
5-(aziridin-1-yl)-2,4-dinitrobenzamide + 2 NADPH + 2 H+
? + 2 NADP+ + H2O
nitrobenzene + 2 NADPH + 2 H+
aniline + 2 NADP+ + H2O
-
reduction of nitrobenzene to aniline occurs through the formation of nitrosobenzene and phenylhydroxylamine as intermediates. More than 99% overall conversion
-
?
nitrobenzene + 2 NADPH + 2 H+
N-hydroxyaniline + 2 NADP+ + H2O
nitrobenzene + 2 NADPH + 2 H+
N-phenylhydroxylamine + 2 NADP+ + H2O
nitrobenzene + 3 NADH + 3 H+
aniline + 3 NAD+ + 2 H2O
-
30% activity compared to 3 NADPH
-
-
?
nitrobenzene + 3 NADPH + 3 H+
aniline + 3 NADP+ + 2 H2O
nitrobenzene + FMN
aniline + ?
-
80% activity compared to 3 NADPH
-
-
?
nitrobenzene + menadione
N-hydroxyaniline + menadiol + H2O
nitrofurazone + 2 NADPH + 2 H+
? + 2 NADP+ + H2O
nitrosobenzene + NADPH + H+
N-hydroxyaniline + NADP+ + H2O
[2-[(4-ethyl-3,5-dimethyl-2H-pyrrol-2-ylidene-?N)(phenyl)methyl]-5-[(2-nitrophenyl)sulfanyl]-1H-pyrrolato-kappaN](difluorido)boron + 2 NADH + 2 H+
[2-([5-[(4-ethyl-3,5-dimethyl-2H-pyrrol-2-ylidene-?N)(phenyl)methyl]-1H-pyrrol-2-yl-kappaN]sulfanyl)anilinato](difluorido)boron + 2 NAD+ + H2O
[2-[(4-ethyl-3,5-dimethyl-2H-pyrrol-2-ylidene-?N)(phenyl)methyl]-5-[(3-nitrophenyl)sulfanyl]-1H-pyrrolato-kappaN](difluorido)boron + 2 NADH + 2 H+
?
[2-[(4-ethyl-3,5-dimethyl-2H-pyrrol-2-ylidene-?N)(phenyl)methyl]-5-[(4-nitrophenyl)sulfanyl]-1H-pyrrolatokappa?N](difluorido)boron + 2 NADH + 2 H+
?
additional information
?
-
(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide + 2 NADPH + 2 H+
(2E)-2-[[5-(hydroxyamino)furan-2-yl]methylidene]hydrazine-1-carboxamide + 2 NADP+
i.e. anti-infective furacilin
-
-
?
(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide + 2 NADPH + 2 H+
(2E)-2-[[5-(hydroxyamino)furan-2-yl]methylidene]hydrazine-1-carboxamide + 2 NADP+
i.e. anti-infective furacilin
-
-
?
1,3-dinitrobenzene + 2 NADPH + 2 H+
? + 2 NADP+
-
-
-
?
1,3-dinitrobenzene + 2 NADPH + 2 H+
? + 2 NADP+
-
-
-
?
2,4-dinitrochlorobenzene + 2 NADPH + 2 H+
? + 2 NADP+ + H2O
-
-
-
-
?
2,4-dinitrochlorobenzene + 2 NADPH + 2 H+
? + 2 NADP+ + H2O
-
recombinant protein, 43% of the activity with 4-chloronitrobenzene
-
?
2,4-dinitrochlorobenzene + 2 NADPH + 2 H+
? + 2 NADP+ + H2O
-
recombinant protein, 43% of the activity with 4-chloronitrobenzene
-
?
2,4-dinitrotoluene + 2 NADPH + 2 H+
? + 2 NADP+ + H2O
-
-
most abundant products are amino-nitrotoluene isomers, plus some hydroxylamino and diaminotoluene isomers
-
?
2,4-dinitrotoluene + 2 NADPH + 2 H+
? + 2 NADP+ + H2O
-
recombinant protein, 98% of the activity with 4-chloronitrobenzene
-
?
2,4-dinitrotoluene + 2 NADPH + 2 H+
? + 2 NADP+ + H2O
-
recombinant protein, 98% of the activity with 4-chloronitrobenzene
-
?
2-chloronitrobenzene + 2 NADPH + 2 H+
2-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
2-chloronitrobenzene + 2 NADPH + 2 H+
2-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
2-chloronitrobenzene + 2 NADPH + 2 H+
2-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
recombinant protein, 17% of the activity with 4-chloronitrobenzene
-
?
2-chloronitrobenzene + 2 NADPH + 2 H+
2-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
recombinant protein, 17% of the activity with 4-chloronitrobenzene
-
?
3-chloronitrobenzene + 2 NADPH + 2 H+
3-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
recombinant protein, 84% of the activity with 4-chloronitrobenzene
-
?
3-chloronitrobenzene + 2 NADPH + 2 H+
3-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
recombinant protein, 84% of the activity with 4-chloronitrobenzene
-
?
3-nitrotoluene + 2 NADPH + 2 H+
N-hydroxy-3-methylaniline + 2 NADP+ + H2O
-
-
-
?
3-nitrotoluene + 2 NADPH + 2 H+
N-hydroxy-3-methylaniline + 2 NADP+ + H2O
-
-
-
?
4-chloronitrobenzene + 2 NADPH + 2 H+
4-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
4-chloronitrobenzene + 2 NADPH + 2 H+
4-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
4-chloronitrobenzene + 2 NADPH + 2 H+
4-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
4-chloronitrobenzene + 2 NADPH + 2 H+
4-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
2.2 mol of NADPH is oxidized per mol of 4-chloronitrobenzene
-
?
4-nitrophenol + 3 NADPH + 3 H+
4-aminophenol + 3 NADP+ + 2 H2O
-
-
-
-
?
4-nitrophenol + 3 NADPH + 3 H+
4-aminophenol + 3 NADP+ + 2 H2O
-
the enzyme can degrade 4-nitrophenol in low temperature environment
-
-
?
5-(aziridin-1-yl)-2,4-dinitrobenzamide + 2 NADPH + 2 H+
? + 2 NADP+ + H2O
-
enzyme activates the dinitrobenzamide cancer prodrug CB1954, i.e. 5-(aziridin-1-yl)-2,4-dinitrobenzamide
-
?
5-(aziridin-1-yl)-2,4-dinitrobenzamide + 2 NADPH + 2 H+
? + 2 NADP+ + H2O
-
enzyme activates the dinitrobenzamide cancer prodrug CB1954, i.e. 5-(aziridin-1-yl)-2,4-dinitrobenzamide
-
?
nitrobenzene + 2 NADPH + 2 H+
N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
nitrobenzene + 2 NADPH + 2 H+
N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
nitrobenzene + 2 NADPH + 2 H+
N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
nitrobenzene + 2 NADPH + 2 H+
N-hydroxyaniline + 2 NADP+ + H2O
-
N-hydroxyaniline is the only product detected
-
?
nitrobenzene + 2 NADPH + 2 H+
N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
nitrobenzene + 2 NADPH + 2 H+
N-hydroxyaniline + 2 NADP+ + H2O
-
N-hydroxyaniline is the only product detected
-
?
nitrobenzene + 2 NADPH + 2 H+
N-hydroxyaniline + 2 NADP+ + H2O
-
recombinant protein, 61% of the activity with 4-chloronitrobenzene
-
?
nitrobenzene + 2 NADPH + 2 H+
N-hydroxyaniline + 2 NADP+ + H2O
-
recombinant protein, 61% of the activity with 4-chloronitrobenzene
-
?
nitrobenzene + 2 NADPH + 2 H+
N-phenylhydroxylamine + 2 NADP+ + H2O
-
-
overall reaction
-
?
nitrobenzene + 2 NADPH + 2 H+
N-phenylhydroxylamine + 2 NADP+ + H2O
-
-
-
-
?
nitrobenzene + 2 NADPH + 2 H+
N-phenylhydroxylamine + 2 NADP+ + H2O
-
-
overall reaction
-
?
nitrobenzene + 3 NADPH + 3 H+
aniline + 3 NADP+ + 2 H2O
-
-
-
-
?
nitrobenzene + 3 NADPH + 3 H+
aniline + 3 NADP+ + 2 H2O
-
100% activity with 3 NADPH
-
-
?
nitrobenzene + 3 NADPH + 3 H+
aniline + 3 NADP+ + 2 H2O
-
the enzyme can degrade 4-nitrophenol in low temperature environment
-
-
?
nitrobenzene + menadione
N-hydroxyaniline + menadiol + H2O
-
-
-
?
nitrobenzene + menadione
N-hydroxyaniline + menadiol + H2O
-
-
-
?
nitrofurazone + 2 NADPH + 2 H+
? + 2 NADP+ + H2O
-
enzyme activates the proantibiotic nitrofurazone
-
?
nitrofurazone + 2 NADPH + 2 H+
? + 2 NADP+ + H2O
-
enzyme activates the proantibiotic nitrofurazone
-
?
nitrofurazone + 2 NADPH + 2 H+
? + 2 NADP+ + H2O
-
-
-
?
nitrosobenzene + NADPH + H+
N-hydroxyaniline + NADP+ + H2O
-
nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme
-
?
nitrosobenzene + NADPH + H+
N-hydroxyaniline + NADP+ + H2O
-
nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme
-
?
[2-[(4-ethyl-3,5-dimethyl-2H-pyrrol-2-ylidene-?N)(phenyl)methyl]-5-[(2-nitrophenyl)sulfanyl]-1H-pyrrolato-kappaN](difluorido)boron + 2 NADH + 2 H+
[2-([5-[(4-ethyl-3,5-dimethyl-2H-pyrrol-2-ylidene-?N)(phenyl)methyl]-1H-pyrrol-2-yl-kappaN]sulfanyl)anilinato](difluorido)boron + 2 NAD+ + H2O
-
-
-
?
[2-[(4-ethyl-3,5-dimethyl-2H-pyrrol-2-ylidene-?N)(phenyl)methyl]-5-[(2-nitrophenyl)sulfanyl]-1H-pyrrolato-kappaN](difluorido)boron + 2 NADH + 2 H+
[2-([5-[(4-ethyl-3,5-dimethyl-2H-pyrrol-2-ylidene-?N)(phenyl)methyl]-1H-pyrrol-2-yl-kappaN]sulfanyl)anilinato](difluorido)boron + 2 NAD+ + H2O
-
-
-
?
[2-[(4-ethyl-3,5-dimethyl-2H-pyrrol-2-ylidene-?N)(phenyl)methyl]-5-[(3-nitrophenyl)sulfanyl]-1H-pyrrolato-kappaN](difluorido)boron + 2 NADH + 2 H+
?
-
-
-
?
[2-[(4-ethyl-3,5-dimethyl-2H-pyrrol-2-ylidene-?N)(phenyl)methyl]-5-[(3-nitrophenyl)sulfanyl]-1H-pyrrolato-kappaN](difluorido)boron + 2 NADH + 2 H+
?
-
-
-
?
[2-[(4-ethyl-3,5-dimethyl-2H-pyrrol-2-ylidene-?N)(phenyl)methyl]-5-[(4-nitrophenyl)sulfanyl]-1H-pyrrolatokappa?N](difluorido)boron + 2 NADH + 2 H+
?
-
-
-
?
[2-[(4-ethyl-3,5-dimethyl-2H-pyrrol-2-ylidene-?N)(phenyl)methyl]-5-[(4-nitrophenyl)sulfanyl]-1H-pyrrolatokappa?N](difluorido)boron + 2 NADH + 2 H+
?
-
-
-
?
additional information
?
-
1.8 mol of NADPH is oxidized per mol of nitrobenzene converted to product
-
-
?
additional information
?
-
1.8 mol of NADPH is oxidized per mol of nitrobenzene converted to product
-
-
?
additional information
?
-
-
enzyme additionally catalyzes reduction of 2,4,6-trinitrotoluene
-
-
?
additional information
?
-
enzyme shows broad substrate acceptance for various nitro compounds such as 1-nitrocyclohexene and aliphatic nitroalkenes (alkene reductase activity), as well as nitrobenzene (nitroreductase activity), with substrate conversion efficiency of >95%
-
-
?
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Wu, J.F.; Jiang, C.Y.; Wang, B.J.; Ma, Y.F.; Liu, Z.P.; Liu, S.J.
Novel partial reductive pathway for 4-chloronitrobenzene and nitrobenzene degradation in Comamonas sp. strain CNB-1
Appl. Environ. Microbiol.
72
1759-1765
2006
Comamonas sp. (Q5XW77), Comamonas sp. Ctestosteroni (Q5XW77)
brenda
Xiao, Y.; Wu, J.F.; Liu, H.; Wang, S.J.; Liu, S.J.; Zhou, N.Y.
Characterization of genes involved in the initial reactions of 4-chloronitrobenzene degradation in Pseudomonas putida ZWL73
Appl. Microbiol. Biotechnol.
73
166-171
2006
Pseudomonas putida (Q3I3W6), Pseudomonas putida, Pseudomonas putida ZWL73 (Q3I3W6), Pseudomonas putida ZWL73
brenda
Rieble, S.; Joshi, D.K.; Gold, M.H.
Aromatic nitroreductase from the basidiomycete Phanerochaete chrysosporium
Biochem. Biophys. Res. Commun.
205
298-304
1994
Phanerodontia chrysosporium
brenda
Berne, C.; Betancor, L.; Luckarift, H.R.; Spain, J.C.
Application of a microfluidic reactor for screening cancer prodrug activation using silica-immobilized nitrobenzene nitroreductase
Biomacromolecules
7
2631-2636
2006
Pseudomonas oleovorans (Q6DLR9), Pseudomonas oleovorans JS45 (Q6DLR9)
brenda
Li, Z.; He, X.; Wang, Z.; Yang, R.; Shi, W.; Ma, H.
In vivo imaging and detection of nitroreductase in zebrafish by a new near-infrared fluorescence off-on probe
Biosens. Bioelectron.
63
112-116
2015
Danio rerio
brenda
Yuan, C.; Hong, Q.; He, J.; Zhou, L.
Isolation and characterization of a nitrobenzene-degrading strain N1 with multiple substrates
Chin. J. Appl. Environ. Biol.
19
1031-1034
2013
Pseudomonas sp., Pseudomonas sp. N1
-
brenda
Somerville, C.C.; Nishino, S.F.; Spain, J.C.
Purification and characterization of nitrobenzene nitroreductase from Pseudomonas pseudoalcaligenes JS45
J. Bacteriol.
177
3837-3842
1995
Pseudomonas oleovorans (Q6DLR9), Pseudomonas oleovorans JS45 (Q6DLR9)
brenda
Ho, E.; Kahng, H.; Oh, K.
Characterization of nitroreductase purified from TNT-degrading bacterium, Pseudomonas sp. HK-6
Korean J. Microbiol. Biotechnol.
32
230-237
2004
Pseudomonas sp. HK-6
-
brenda
Yang, Y.; Lin, J.; Wei, D.
Heterologous overexpression and biochemical characterization of a nitroreductase from Gluconobacter oxydans 621H
Mol. Biotechnol.
58
428-440
2016
Gluconobacter oxydans (Q5FSN8), Gluconobacter oxydans 621H (Q5FSN8)
brenda
Yanto, Y.; Hall, M.; Bommarius, A.S.
Nitroreductase from Salmonella typhimurium: characterization and catalytic activity
Org. Biomol. Chem.
8
1826-1832
2010
Salmonella enterica subsp. enterica serovar Typhimurium (P15888)
brenda
Isayev, O.; Crespo-Hernandez, C.E.; Gorb, L.; Hill, F.C.; Leszczynski, J.
In silico structure-function analysis of E. cloacae nitroreductase
Proteins
80
2728-2741
2012
Enterobacter cloacae (Q01234)
brenda
Wang, Y.; Hou, Y.; Wang, Y.; Zheng, L.; Wang, Q.
A novel cold-adapted nitroreductase from Psychrobacter sp. ANT206 Heterologous expression, characterization and nitrobenzene reduction capacity
Enzyme Microb. Technol.
131
109434
2019
Psychrobacter sp. ANT206
brenda
Wang, Y.; Hou, Y.; Wang, Q.; Wang, Y.
The elucidation of the biodegradation of nitrobenzene and p-nitrophenol of nitroreductase from Antarctic psychrophile Psychrobacter sp. ANT206 under low temperature
J. Hazard. Mater.
413
125377
2021
Psychrobacter sp. ANT206
brenda
Gao, J.; Yin, X.; Li, M.; Chen, J.A.; Tan, J.; Zhao, Z.; Gu, X.
Rational design of fluorescent probes for targeted in vivo nitroreductase visualization
Org. Biomol. Chem.
18
4744-4747
2020
Geobacter metallireducens (Q39RS1), Geobacter metallireducens DSM 7210 (Q39RS1)
brenda