Information on EC 1.6.99.5 - NADH dehydrogenase (quinone)

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY
1.6.99.5
-
RECOMMENDED NAME
GeneOntology No.
NADH dehydrogenase (quinone)
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
NADH + H+ + acceptor = NAD+ + reduced acceptor
show the reaction diagram
-
-
-
-
NADH + H+ + acceptor = NAD+ + reduced acceptor
show the reaction diagram
ping-pong mechanism
-
NADH + H+ + acceptor = NAD+ + reduced acceptor
show the reaction diagram
ping-pong mechanism
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Oxidative phosphorylation
-
-
SYSTEMATIC NAME
IUBMB Comments
NADH:(quinone-acceptor) oxidoreductase
Menaquinone can act as acceptor. Inhibited by AMP and 2,4-dinitrophenol but not by dicoumarol or folic acid derivatives.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D-diaphorase
-
-
-
-
dehydrogenase, reduced nicotinamide adenine dinucleotide (quinone)
-
-
-
-
DPNH-menadione reductase
-
-
-
-
FMN-dependent NADH-quinone reductase
-
-
FMN-dependent NADH:quinone oxidoreductase
D9PVS9
-
FMN-dependent NADH:quinone oxidoreductase
Methanothermobacter marburgensis DSM 2133
D9PVS9
-
-
H+(NA+)-translocating NADH-quinone oxidoreductase
-
-
MmNQO
Methanothermobacter marburgensis DSM 2133
D9PVS9
-
-
NADH-dependent 1,4-benzoquinone reductase
-
-
NADH-Q oxidoreductase
Q7RJ45
-
NADH-quinone oxidoreductase
-
-
-
-
NADH-quinone oxidoreductase-1
-
-
NADH-quinone oxidoreductases
Q7RJ45
-
NADH:menadione oxidoreductase
-
-
NADH:quinone oxidoreductase
-
-
NADH:ubiquinone oxidoreductase
-
-
NDH-1
Thermus thermophilus HB-8
-
-
-
NUO1
-
-
-
-
NUO10
-
-
-
-
NUO11
-
-
-
-
NUO12
-
-
-
-
NUO13
-
-
-
-
NUO14
-
-
-
-
NUO2
-
-
-
-
NUO3/NUO4
-
-
-
-
NUO5
-
-
-
-
NUO6
-
-
-
-
NUO7
-
-
-
-
NUO8
-
-
-
-
NUO9
-
-
-
-
proton-translocating NADH-quinone oxidoreductase
-
-
proton-translocating NADH-quinone oxidoreductase
-
-
proton-translocating NADH-quinone oxidoreductase
-
-
proton-translocating NADH-quinone oxidoreductase
-
-
proton-translocating NADH-quinone oxidoreductase
Thermus thermophilus HB-8
-
-
-
type 2 NADH:quinone oxidoreductase complex
-
-
type 2 NADH:quinone oxidoreductase complex
Methylococcus capsulatus Bath
-
-
-
type II NADH:menaquinone oxidoreductase
-
-
type II NADH:quinone oxidoreductase
-
-
CAS REGISTRY NUMBER
COMMENTARY
37256-36-3
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
YN-1
-
-
Manually annotated by BRENDA team
growth in presence of menadione
-
-
Manually annotated by BRENDA team
halophilic bacterium
-
-
-
Manually annotated by BRENDA team
halophilic bacterium
strain AR-I
-
-
Manually annotated by BRENDA team
halophilic bacterium AR-I
strain AR-I
-
-
Manually annotated by BRENDA team
Methanothermobacter marburgensis DSM 2133
-
SwissProt
Manually annotated by BRENDA team
Methylococcus capsulatus Bath
Bath
-
-
Manually annotated by BRENDA team
strain 37b4
-
-
Manually annotated by BRENDA team
subunit NuoL
SwissProt
Manually annotated by BRENDA team
subunit NuoM
SwissProt
Manually annotated by BRENDA team
subunit NuoN
SwissProt
Manually annotated by BRENDA team
Rhodobacter capsulatus 37b4
strain 37b4
-
-
Manually annotated by BRENDA team
hog
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
-
PfNDH2 is a principal electron donor to the electron transport chain, linking fermentative metabolism to the generation of mitochondrial electrochemical membrane potential, an essential function for parasite viability. PfNDH2 is involved in the redox reaction of NADH oxidation with subsequent quinol production
metabolism
-
mitochondrial electron transfer chain and the role of PfNDH2 and bc1, overview
additional information
-
structure-activity relationship, overview
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 ferricyanide + NADH
2 ferrocyanide + NAD+ + H+
show the reaction diagram
-
-
-
-
?
2 ferricyanide + NADH
2 ferrocyanide + NAD+ + H+
show the reaction diagram
-
-
-
-
?
2 ferricyanide + NADH
2 ferrocyanide + NAD+ + H+
show the reaction diagram
-
-
-
-
?
2 ferricyanide + NADH
2 ferrocyanide + NAD+ + H+
show the reaction diagram
-
-
-
-
?
2 ferricyanide + NADH
2 ferrocyanide + NAD+ + H+
show the reaction diagram
-
-
-
?
2 ferricyanide + NADH
2 ferrocyanide + NAD+ + H+
show the reaction diagram
-
-
-
-
?
2 ferricytochrome c + NADH
2 ferrocytochrome c + NAD+ + H+
show the reaction diagram
-
-
-
-
?
2 ferricytochrome c + NADH
2 ferrocytochrome c + NAD+ + H+
show the reaction diagram
-
-
-
-
?
2 ferricytochrome c + NADH
2 ferrocytochrome c + NAD+ + H+
show the reaction diagram
-
-
-
-
?
2 ferricytochrome c + NADH
2 ferrocytochrome c + NAD+ + H+
show the reaction diagram
-
-
-
-
?
3-acetylpyridine-deamino-NADH + 2,6-dichlorophenol indophenol
3-acetylpyridine-deamino-NAD+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
halophilic bacterium, halophilic bacterium AR-I
-
-
-
-
?
3-acetylpyridine-NADH + 2,6-dichlorophenolindophenol
3-acetylpyridine-NAD+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
halophilic bacterium, halophilic bacterium AR-I
-
-
-
-
?
deamino-NADH + 2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone
?
show the reaction diagram
-
-
-
-
?
deamino-NADH + K3Fe(CN)6
?
show the reaction diagram
-
-
-
-
?
NADH + 1,2-naphthoquinone
NAD+ + 1,2-naphthoquinol
show the reaction diagram
-
-
-
?
NADH + 1,4-benzoquinone
NAD+ + 1,4-benzoquinol
show the reaction diagram
Q8X172
-
-
?
NADH + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
NAD+ + ?
show the reaction diagram
-
-
-
-
?
NADH + 2,5-dimethoxy-1,4-benzoquinone
NAD+ + 2,5-dimethoxy-1,4-benzoquinol
show the reaction diagram
-
-
-
-
?
NADH + 2,5-dimethoxy-1,4-benzoquinone
NAD+ + 2,5-dimethoxy-1,4-benzoquinol
show the reaction diagram
Q8X172
2,5-dimethoxy-1,4-benzoquinone produced by Gleophyllum trabeum
-
?
NADH + 2,5-toluquinone
NAD+ + 2,5-toluquinol
show the reaction diagram
-
-
-
?
NADH + 2,6-dimethoxy-1,4-benzoquinone
NAD+ + 2,6-dimethoxy-1,4-benzoquinol
show the reaction diagram
Q8X172
-
-
?
NADH + 2,6-dimethoxy-1,4-benzoquinone
NAD+ + 2,6-dimethoxy-1,4-benzoquinol
show the reaction diagram
-
-
-
-
?
NADH + 2-methoxy-1,4-benzoquinone
NAD+ + 2-methoxy-1,4-benzoquinol
show the reaction diagram
Q8X172
-
-
?
NADH + 2-methyl-1,4-naphthoquinone
NAD+ + 2-methyl-1,4-naphthoquinol
show the reaction diagram
-
-
-
-
?
NADH + 2-methyl-1,4-naphthoquinone
NAD+ + 2-methyl-1,4-naphthoquinol
show the reaction diagram
-
-
-
-
?
NADH + 2-methyl-1,4-naphthoquinone
NAD+ + 2-methyl-1,4-naphthoquinol
show the reaction diagram
-
-
-
-
?
NADH + 2-methyl-1,4-naphthoquinone
NAD+ + 2-methyl-1,4-naphthoquinol
show the reaction diagram
-
-
-
-
?
NADH + 2-methyl-1,4-naphthoquinone
NAD+ + 2-methyl-1,4-naphthoquinol
show the reaction diagram
-
-
-
-
?
NADH + 2-methyl-1,4-naphthoquinone
NAD+ + 2-methyl-1,4-naphthoquinol
show the reaction diagram
halophilic bacterium, halophilic bacterium AR-I
-
i.e. menadione, about 60% of the activity with 2,6-dichlorophenolindophenol
-
-
?
NADH + 4,5-dimethoxy-1,2-benzoquinone
NAD+ + 4,5-dimethoxy-1,2-benzoquinol
show the reaction diagram
Q8X172
4,5-dimethoxy-1,2-benzoquinone produced by Gleophyllum trabeum
-
?
NADH + 5-hydroxy-1,4-naphthoquinone
NAD+ + 5-hydroxy-1,4-naphthoquinol
show the reaction diagram
halophilic bacterium, halophilic bacterium AR-I
-
i.e. juglone, about 55% of the activity with 2,6-dichlorophenolindophenol
-
-
?
NADH + benzoquinone
NAD+ + benzoquinol
show the reaction diagram
-
-
-
-
?
NADH + benzoquinone
NAD+ + benzoquinol
show the reaction diagram
-
-
-
?
NADH + benzoquinone
NAD+ + benzoquinol
show the reaction diagram
-
-
-
?
NADH + benzoquinone
NAD+ + benzoquinol
show the reaction diagram
-
benzoquinone is the best acceptor
-
?
NADH + benzoquinone
NAD+ + benzoquinol
show the reaction diagram
-
ping-pong kinetics, two electron transfer mechanism
-
-
?
NADH + duroquinone
NAD+ + duroquinol
show the reaction diagram
Methylococcus capsulatus, Methylococcus capsulatus Bath
-
-
-
-
?
NADH + Fe(CN)63-
NAD+ + Fe(CN)64-
show the reaction diagram
-
-
-
-
?
NADH + ferricyanide
NAD+ + H+ + ferrocyanide
show the reaction diagram
D9PVS9
-
-
-
?
NADH + ferrocenium hexafluorophosphate
NAD+ + H+ + ferrocene
show the reaction diagram
D9PVS9
highest specific activity with NADH and ferrocenium hexafluorophosphate as electron acceptor
-
-
?
NADH + H+ + 1,4-benzoquinone
NAD+ + 1,4-benzoquinol
show the reaction diagram
Methanothermobacter marburgensis, Methanothermobacter marburgensis DSM 2133
D9PVS9
-
-
-
?
NADH + H+ + 1,4-naphthoquinone
NAD+ + 1,4-naphthoquinol
show the reaction diagram
-
-
-
?
NADH + H+ + 1,4-naphthoquinone
NAD+ + 1,4-naphthoquinol
show the reaction diagram
-
1,4-naphthoquinone is one of the best acceptors
-
?
NADH + H+ + acceptor
NAD+ + reduced acceptor
show the reaction diagram
-
-
-
-
?
NADH + H+ + atovaquone
NAD+ + reduced atovaquone
show the reaction diagram
-
atovaquone is 2-[4-(4-chlorophenyl)cyclohexyl]-3-hydroxynaphthalene-1,4-dione
-
-
?
NADH + H+ + clofazimine
NAD+ + reduced clofazimine
show the reaction diagram
-
NDH-2 is the only respiratory chain oxidoreductase capable of reducing clofazimine
-
-
?
NADH + H+ + menaquinone
NAD+ + menaquinol
show the reaction diagram
-
-
-
-
?
NADH + H+ + oxidized 2,6-dichloroindophenol
NAD+ + reduced 2,6-dichloroindophenol
show the reaction diagram
D9PVS9
-
-
-
?
NADH + H+ + ubiquinone-1
NAD+ + ubiquinol-1
show the reaction diagram
Methanothermobacter marburgensis, Methanothermobacter marburgensis DSM 2133
D9PVS9
-
-
-
?
NADH + H+ + ubiquinone-10
NAD+ + ubiquinol-10
show the reaction diagram
Methanothermobacter marburgensis, Methanothermobacter marburgensis DSM 2133
D9PVS9
-
-
-
?
NADH + menadione
NAD+ + menadiol
show the reaction diagram
-
-
-
?
NADH + menadione
NAD+ + menadiol
show the reaction diagram
-
-
-
?
NADH + menadione
NAD+ + menadiol
show the reaction diagram
-
menadione is one of the best acceptors
-
?
NADH + menadione
NAD+ + menadiol
show the reaction diagram
-
menadione is a poor electron acceptor
-
?
NADH + menadione
NAD+ + reduced menadione
show the reaction diagram
-
-
-
-
?
NADH + menaquinone
NAD+ + menaquinol
show the reaction diagram
-
-
-
-
?
NADH + methyl-p-benzoquinone
NAD+ + methyl-p-benzoquinol
show the reaction diagram
-
-
-
?
NADH + oxidized 2,6-dichlorophenolindophenol + H+
NAD+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
NADH + oxidized 2,6-dichlorophenolindophenol + H+
NAD+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
NADH + oxidized 2,6-dichlorophenolindophenol + H+
NAD+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
NADH + oxidized 2,6-dichlorophenolindophenol + H+
NAD+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
NADH + oxidized 2,6-dichlorophenolindophenol + H+
NAD+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
NADH + oxidized 2,6-dichlorophenolindophenol + H+
NAD+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
halophilic bacterium
-
-
-
-
?
NADH + oxidized 2,6-dichlorophenolindophenol + H+
NAD+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
NADH + oxidized 2,6-dichlorophenolindophenol + H+
NAD+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
specific for NADH
-
-
?
NADH + oxidized 2,6-dichlorophenolindophenol + H+
NAD+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
Rhodobacter capsulatus 37b4
-
specific for NADH
-
-
?
NADH + oxidized 2,6-dichlorophenolindophenol + H+
NAD+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
halophilic bacterium AR-I
-
-
-
-
?
NADH + paraquat
NAD+ + ?
show the reaction diagram
-
the enzyme is a novel oxidation-reduction system participating in paraquat toxicity
-
?
NADH + plastoquinone
NAD+ + reduced plastoquinone
show the reaction diagram
-
-
-
-
?
NADPH + H+ + 1,4-benzoquinone
NADP+ + 1,4-benzoquinol
show the reaction diagram
Methanothermobacter marburgensis, Methanothermobacter marburgensis DSM 2133
D9PVS9
-
-
-
?
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
NADP+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
NADPH + H+ + ubiquinone-1
NADP+ + ubiquinol-1
show the reaction diagram
Methanothermobacter marburgensis, Methanothermobacter marburgensis DSM 2133
D9PVS9
-
-
-
?
thionicotinamide-NADH + H+ + oxidized 2,6-dichlorophenolindophenol
thionicotinamide-NAD+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
halophilic bacterium
-
-
-
-
?
menadione + NADH + H+
menadiol + NAD+
show the reaction diagram
-
menadione is reduced by a two-electron transfer reaction
-
-
?
additional information
?
-
-
the enzyme can not utilize free flavins, cytochrome c or molecular oxygen as an electron acceptor
-
-
-
additional information
?
-
-
enzyme is involved in the respiratory chain
-
-
-
additional information
?
-
Q8X172
the enzyme may function in quinone detoxification, it is also hypothesized that the fungus has recruited it to drive extracellular oxyradical production
-
-
-
additional information
?
-
-
activity is increased in the presence of 2,6-dimethoxy-1,4-benzoquinone and, to a lesser extent, vanillic acid. Expression is time-dependent and influenced by nitrogen levels
-
-
-
additional information
?
-
-
no direct involvement of electron carriers in H+ (Na+) tranlocation
-
-
-
additional information
?
-
-
deamino-NADH does not serve as substrate
-
-
-
additional information
?
-
-
enzyme is most active toward naphtho- and benzoquinones, whereas ferricyanide and dichlorophenolindophenol are less efficient. Enzyme reacts with ubiquinone Q-l, but ubiquinones with a long side-chain such as Q-5 and Q-10 are virtually inactive as electron acceptors
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
NADH + 2,5-dimethoxy-1,4-benzoquinone
NAD+ + 2,5-dimethoxy-1,4-benzoquinol
show the reaction diagram
Q8X172
2,5-dimethoxy-1,4-benzoquinone produced by Gleophyllum trabeum
-
?
NADH + 4,5-dimethoxy-1,2-benzoquinone
NAD+ + 4,5-dimethoxy-1,2-benzoquinol
show the reaction diagram
Q8X172
4,5-dimethoxy-1,2-benzoquinone produced by Gleophyllum trabeum
-
?
NADH + H+ + acceptor
NAD+ + reduced acceptor
show the reaction diagram
-
-
-
-
?
NADH + H+ + atovaquone
NAD+ + reduced atovaquone
show the reaction diagram
-
atovaquone is 2-[4-(4-chlorophenyl)cyclohexyl]-3-hydroxynaphthalene-1,4-dione
-
-
?
NADH + H+ + clofazimine
NAD+ + reduced clofazimine
show the reaction diagram
-
NDH-2 is the only respiratory chain oxidoreductase capable of reducing clofazimine
-
-
?
NADH + H+ + menaquinone
NAD+ + menaquinol
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
enzyme is involved in the respiratory chain
-
-
-
additional information
?
-
Q8X172
the enzyme may function in quinone detoxification, it is also hypothesized that the fungus has recruited it to drive extracellular oxyradical production
-
-
-
additional information
?
-
-
activity is increased in the presence of 2,6-dimethoxy-1,4-benzoquinone and, to a lesser extent, vanillic acid. Expression is time-dependent and influenced by nitrogen levels
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3-acetylpyridine-deamino-NADH
halophilic bacterium
-
-
3-acetylpyridine-NADH
halophilic bacterium
-
-
FAD
halophilic bacterium
-
FAD represents 85% of the total flavin
FAD
-
contains 1 mol FAD per subunit
FAD
-
purified enzyme has an absolute requirement for FAD. Km-value for the reaction with 2,6-dichlorophenol indophenol is 0.004 mM, the Km-value for the reaction with ferricyanide is 0.006 mM, the KM-value for the reaction with menadione is 0.003 mM
FAD
-
half-maximum activity at 16.5 microM
FMN
Q8X172
0.9 mol of FMN per mol of subunit
FMN
-
preferred over FAD, half-maximum activity at 0.54 microM
NADH
-
specific for NADH
NADH
-
specific for NADH
NADH
-
specific for NADH
NADH
halophilic bacterium
-
-
NADH
-
specific for NADH
NADH
-
specific for
NADH
-
specific for
NADH
-
specific for
NADH
Q8X172
specific for
NADH
-
ndh is selective for only NADH
NADPH
-
activity is below 2% of the NADH-oxidizing activity
NADPH
D9PVS9
the activity with NADPH as substrate is 2- to 20-fold lower compared to NADH
Thionicotinamide-NADH
halophilic bacterium
-
-
FMN
D9PVS9
the active sites of both monomers are situated at the dimer interface, each with a non-covalently bound FMN molecule bound approximately 8 A below the molecules surface. The flavin isoalloxazine ring is firmly bound by the protein
additional information
-
purified enzyme contains no flavin
-
additional information
-
no cofactor: NADPH
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
Triton X-100-activated enzyme form is stimulated by several divalent cations without specificity
Iron
-
all four cysteines, Cys256, Cys259, Cys263 and Cys291 participate in the ligation of a [4Fe-4S] cluster. The CXXCXXXCX27C motif in the Nqo3 subunit most likely ligates the [4Fe-4S] cluster
Iron
-
contains 9.3 nM per mg protein
Iron
-
characterization of the iron-sulfur cluster N7 in the subunit NuoG, a [4Fe-4S] cluster ligates the N1c motif
K+
-
stimulates activity with electron acceptors other than cytochrome c
K+
-
stimulates activity with electron acceptors other than cytochrome c
K+
-
KCN stimulates
Mg2+
-
Triton X-100-activated enzyme form is stimulated by several divalent cations without specificity
Mn2+
-
Triton X-100-activated enzyme form is stimulated by several divalent cations without specificity
Na+
halophilic bacterium
-
NaCl stimulates, 43% of maximal activity in presence of 2.5 M NaCl
Na+
-
stimulates activity with electron acceptors other than cytochrome c
Na+
-
stimulates activity with electron acceptors other than cytochrome c
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1-hydroxy-2-dodecyl-4(1H)-quinolone
-
-
1-hydroxy-2-methyl-3-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
1-hydroxy-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
1-hydroxy-3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
1-hydroxy-3-methyl-2-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
-
-
2,4-Dinitrophenol
-
52% inhibition at 0.16 mM and 100% inhibition at 0.33 mM
2,4-Dinitrophenol
-
not inhibitory
2,6-dichlorophenol indophenol
halophilic bacterium
-
above 0.07 mM
2,6-dichlorophenol indophenol
halophilic bacterium
-
product inhibitor
2-(2',4'-difluorobiphenyl-4-yl)-3-methylquinolin-4(1H)-one
-
-
2-(2'-fluorobiphenyl-4-yl)-3-methylquinolin-4(1H)-one
-
-
2-(3-[4-[2-(diethylnitroryl)ethoxy]benzyl]phenyl)-3-methylquinolin-4(1H)-one
-
-
2-(3-[4-[2-(diethylnitroryl)ethoxy]phenoxy]phenyl)-3-methylquinolin-4(1H)-one
-
-
2-(4-benzylphenyl)-3-methylquinolin-4(1H)-one
-
-
2-(4-bromophenyl)-3-methyl-1,8-naphthyridin-4(1H)-one
-
-
2-(4-hydroxyphenyl)quinolin-4(1H)-one
-
-
2-(4-methoxyphenyl)-3-methyl-1,8-naphthyridin-4(1H)-one
-
-
2-(4-methoxyphenyl)quinolin-4(1H)-one
-
-
2-(4-[4-[2-(diethylnitroryl)ethoxy]phenoxy]phenyl)-3-methylquinolin-4(1H)-one
-
-
2-(hydroxymethyl)-3-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
-
-
2-methyl-3-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
2-methyl-3-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
-
-
2-[3,5-difluoro-4'-(trifluoromethoxy)biphenyl-4-yl]-3-methylquinolin-4(1H)-one
-
-
2-[4-(4-chlorophenoxy)phenyl]-3-methylquinolin-4(1H)-one
-
-
2-[4-(4-fluorobenzyl)phenyl]-3-methylquinolin-4(1H)-one
-
-
2-[4-(4-methoxybenzyl)phenyl]-3-methylquinolin-4(1H)-one
-
-
2-[4-(trifluoromethoxy)phenyl]quinolin-4(1H)-one
-
-
2-[4-(trifluoromethyl)phenyl]quinolin-4(1H)-one
-
-
2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
2-[6-(2,4-difluorophenyl)pyridin-3-yl]-3-methylquinolin-4(1H)-one
-
-
2-[6-(3-fluorophenyl)pyridin-3-yl]-3-methylquinolin-4(1H)-one
-
-
2-[6-(3-hydroxyphenyl)pyridin-3-yl]-3-methylquinolin-4(1H)-one
-
-
2-[6-(3-methoxyphenyl)pyridin-3-yl]-3-methylquinolin-4(1H)-one
-
-
2-[6-(4-hydroxyphenyl)pyridin-3-yl]-3-methylquinolin-4(1H)-one
-
-
3-(hydroxymethyl)-2-[4'-(trifluoromethoxy)biphenyl-4-yl]quinolin-4(1H)-one
-
-
3-(hydroxymethyl)-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
3-chloro-2-(4-methoxyphenyl)quinolin-4(1H)-one
-
-
3-chloro-2-[4-(trifluoromethoxy)phenyl]quinolin-4(1H)-one
-
-
3-chloro-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
3-methoxy-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
3-methyl-2-[2'-(trifluoromethyl)biphenyl-4-yl]quinolin-4(1H)-one
-
-
3-methyl-2-[3-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
3-methyl-2-[4'-(trifluoromethoxy)biphenyl-4-yl]quinolin-4(1H)-one
-
-
3-methyl-2-[4-(trifluoromethoxy)phenyl]-1,8-naphthyridin-4(1H)-one
-
-
3-methyl-2-[4-(trifluoromethoxy)phenyl]quinolin-4(1H)-one
-
-
3-methyl-2-[4-(trifluoromethyl)phenyl]-1,8-naphthyridin-4(1H)-one
-
-
3-methyl-2-[4-(trifluoromethyl)phenyl]quinolin-4(1H)-one
-
-
3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]-1,8-naphthyridin-4(1H)-one
-
-
3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]-6-(trifluoromethyl)quinolin-4(1H)-one
-
-
3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
3-methyl-2-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
-
-
3-methyl-2-[6-[3-(trifluoromethyl)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]-6-(trifluoromethyl)quinolin-4(1H)-one
-
-
3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]-7-(trifluoromethyl)quinolin-4(1H)-one
-
-
3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
3-methyl-2-[6-[4-(trifluoromethyl)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
3-methyl-6-(methylsulfonyl)-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
3-methyl-7-(trifluoromethoxy)-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
3-pyridine aldehyde NADH
halophilic bacterium
-
competitive with respect to NADH
3-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
-
-
5-(N-ethyl-N-isopropyl) amiloride
-
IC50: above 0.001 mM, inhibition at 0.1 mM is 30% or less
5-(N-ethyl-N-isopropyl) amiloride
-
IC50: 0.017 mM
5-(N-ethyl-N-isopropyl) amiloride
-
IC50: above 0.1 mM
5-(N-ethyl-N-isopropyl) amiloride
-
IC50: 0.063 mM
5-(N-methyl-N-isobutyl)amiloride
-
IC50: about 0.001 mM
5-(N-methyl-N-isobutyl)amiloride
-
IC50: 0.017 mM
5-(N-methyl-N-isobutyl)amiloride
-
0.1 mM, inhibition is 30% or less, enzyme NDH-2; IC50: 0.045 mM
5-(N-methyl-N-isobutyl)amiloride
-
IC50: 0.02 mM
5-hydroxy-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
5-methoxy-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
6,7-difluoro-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
6,7-difluoro-3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
6,7-difluoro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
6,8-difluoro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
6-chloro-1-hydroxy-3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
6-chloro-2-[3-(4-fluorobenzyl)phenyl]-3-methylquinolin-4(1H)-one
-
-
6-chloro-3-(hydroxymethyl)-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
6-chloro-3-methyl-2-[3-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
6-chloro-3-methyl-2-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
-
-
6-chloro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
6-chloro-7-fluoro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
6-fluoro-3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
6-fluoro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
6-hydroxy-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
7-chloro-3-methyl-2-(4-(4-(trifluoromethoxy)benzyl)phenyl)quinolin-4(1H)-one
-
i.e. CK-2-68, shows potent in vivo antimalarial activity after oral administration, is selective for PfNDH2 over other respiratory enzymes, and demonstrates low cytotoxicity and high metabolic stability in the presence of human liver microsomes
-
7-chloro-3-methyl-2-[3-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
7-chloro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
7-chloro-6-fluoro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
7-hydroxy-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
7-methoxy-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
8-methoxy-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
adenine
-
3 mM, 60% inhibition in the reaction with 0.04 mM 2,6-dichlorophenolindophenol and 0.032 mM NADH
ADP
-
10 mM, 90% inhibition in the reaction with 0.04 mM 2,6-dichlorophenolindophenol and 0.032 mM NADH, competitive
AMP
halophilic bacterium
-
competitive inhibition of NADH oxidation
AMP
-
10 mM, 98% inhibition in the reaction with 0.04 mM 2,6-dichlorophenolindophenol and 0.032 mM NADH, competitive
AMP
-
at high concentrations
ATP
-
10 mM, 50% inhibition in the reaction with 0.04 mM 2,6-dichlorophenolindophenol and 0.032 mM NADH, competitive
benzamil
-
IC50: about 0.001 mM
benzamil
-
IC50: 0.005 mM
benzamil
-
0.1 mM, inhibition is 30% or less, enzyme NDH-2; IC50: 0.045 mM
benzamil
-
IC50: 0.042 mM
cAMP
-
10 mM, 76% inhibition in the reaction with 0.04 mM 2,6-dichlorophenolindophenol and 0.032 mM NADH
capsaicin-40
-
-
chlorpromazine
-
-
Cibacron blue
-
competitive
deoxyadenosine
-
10 mM, 32% inhibition in the reaction with 0.04 mM 2,6-dichlorophenolindophenol and 0.032 mM NADH
deoxyAMP
-
10 mM, 41% inhibition in the reaction with 0.04 mM 2,6-dichlorophenolindophenol and 0.032 mM NADH
dicoumarol
-
at high concentrations
dicoumarol
-
competitive with NADH
dicoumarol
-
competitive
dicoumarol
-
3 microM, 85% inhibition
diphenylene iodonium chloride
-
-
ethyl 2-(4'-chlorobiphenyl-4-yl)-4-oxo-1,4-dihydroquinoline-3-carboxylate
-
-
ethyl 2-methyl-3-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4-yl carbonate
-
-
ethyl 3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4-yl carbonate
-
-
ethyl 3-methyl-2-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4-yl carbonate
-
-
ethyl 4-oxo-2-[4'-(trifluoromethoxy)biphenyl-4-yl]-1,4-dihydroquinoline-3-carboxylate
-
-
ethyl 4-oxo-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]-1,4-dihydroquinoline-3-carboxylate
-
-
flavone
-
IC50: 0.158 mM, weak inhibitor
FMN
-
inactivates
HgCl2
-
completely inhibits at 0.1 mM
hydroxy-2-dodecyl-4-(1H)-quinolone
-
-
-
methyl 4-oxo-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]-1,4-dihydroquinoline-3-carboxylate
-
-
N,N'-dicyclohexylcarbodiimide
-
2 mM, 79% inhibition of wild-type enzyme after 4 h at room temperature, 74% inhibition of mutant enzyme K46A, 77% inhibition of mutant enzyme E51A, 71% inhibition of mutant enzyme D79A, 76% inhibitiuon of mutant enzyme D79N, 50% inhibition of mutant enzyme E81A, 30% inhibition of mutant enzyme E81Q
NAD+
halophilic bacterium
-
competitive inhibition of NADH oxidation
NAD+
halophilic bacterium
-
product inhibitor, competitive with NADH, non-competitive with 2,6-dichlorophenolindophenol
NAD+
-
effectively competitive
NADH
-
inactivates
o-phenanthroline
-
-
p-chloromercuribenzoate
-
completely inhibits at 0.1 mM
p-hydroxymercuribenzoate
-
less than 40% inhibition at 0.001 mM, 100% inhibition at 0.5 mM
p-Hydroxymercuriphenyl sulfonate
-
2 mM, 20% inhibition of activity with ferricyanide
phenothiazine
-
1 mM, complete inhibition
phosphate
-
no deactivation
riboflavin
-
inactivates
rotenone
-
-
rotenone
-
not inhibitory
Thenoyltrifluoroacetone
-
-
Trifluoperazine
-
-
methyl 6-chloro-4-oxo-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]-1,4-dihydroquinoline-3-carboxylate
-
-
additional information
-
activation by monovalent cations is due to increase of affinity of the enzyme for substrates
-
additional information
-
no inhibition by 0.1 mM 5-(N-ethyl-N-isopropyl) amiloride, enzyme NDH-2
-
additional information
-
no inhibition by 1 mM ZnSO4, MgSO4, CuSO4, MnSO4, EDTA or NaN3
-
additional information
-
no inhibition by rotenone, piericidin and pyridaben
-
additional information
-
inhibitor screening using hydroxy-2-dodecyl-4-(1H)-quinolone as template, overview. Selection of 2-bisaryl 3-methyl quinolones
-
additional information
-
identification, design and biological evaluation of heterocyclic quinolones targeting Plasmodium falciparum type II NADH:quinone oxidoreductase, PfNDH2, overview
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Cl-
halophilic bacterium
-
stimulates
diphosphatidylglycerol
-
most effective lipid activator. Maximal activation at pH 7 and at 0C
NO3-
halophilic bacterium
-
stimulates
phosphatidylglycerol
-
most effective lipid activator. Maximal activation at pH 7 and 0C
Phospholipid
-
slightly increases activity
SO42-
halophilic bacterium
-
stimulates
spermidine
-
enzyme optimally activated by phospholipids is additionally activated 2fold
Triton X-100
-
enzyme is inactive in absence of detergent or phospholipid. Triton X-100 gives optimal activity
FMN
-
activation of soluble enzyme
additional information
-
no stimulation with FAD or FMN
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0481
1,4-benzoquinone
D9PVS9
cosubstrate: NADPH, pH 7.4, temperature not specified in the publication
0.0565
1,4-benzoquinone
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
0.0162
2,3-dimethoxy-5-methyl-1,4-benzoquinone
-
pH 6.0, room temperature
0.005 - 0.007
2,5-dimethoxy-1,4-benzoquinone
Q8X172
-
0.0058
2,5-dimethoxy-1,4-benzoquinone
-
pH 6.0, room temperature
0.03
2,6-dichlorophenol indophenol
halophilic bacterium
-
-
0.0029
2,6-dichlorophenolindophenol
-
-
0.0172
2,6-dichlorophenolindophenol
-
pH 6.0, room temperature
0.063
2,6-dichlorophenolindophenol
-
-
0.11
2,6-dichlorophenolindophenol
-
-
0.0068
2,6-dimethoxy-1,4-benzoquinone
-
pH 6.0, room temperature
0.021
2-methyl-1,4-naphthoquinone
halophilic bacterium
-
-
0.035
2-methyl-1,4-naphthoquinone
-
-
0.005 - 0.007
4,5-dimethoxy-1,2-benzoquinone
Q8X172
-
0.133
5-hydroxy-1,4-naphthoquinone
halophilic bacterium
-
-
0.0038
benzoquinone
-
pH 6.0, room temperature
0.002
cytochrome c
-
-
0.015
cytochrome c
-
-
0.2158
Fe(CN)63-
-
pH 6.0, room temperature
0.0748
ferricyanide
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
2.1
ferricyanide
-
-
0.0648
ferrocenium hexafluorophosphate
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
0.002
menadione
-
pH 7.5, 30C
0.1
menadione
-
-
0.012
NADH
-
-
0.017
NADH
halophilic bacterium
-
reaction with 5-hydroxy-1,4-naphthoquinone
0.022
NADH
halophilic bacterium
-
reaction with 2,6-dichlorophenolindophenol
0.031
NADH
-
at pH 7
0.034
NADH
halophilic bacterium
-
reaction with 2-methyl-1,4-naphthoquinone
0.0363
NADH
-
pH 6.0, room temperature
0.085 - 0.09
NADH
Q8X172
-
0.132
NADH
-
pH 7.5, 30C
0.0175
oxidized 2,6-dichloroindophenol
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
0.0968
ubiquinone-1
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
0.112
ubiquinone-1
D9PVS9
cosubstrate: NADPH, pH 7.4, temperature not specified in the publication
0.0337
Ubiquinone-10
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
6
1,4-benzoquinone
D9PVS9
cosubstrate: NADPH, pH 7.4, temperature not specified in the publication
9.74
1,4-benzoquinone
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
5300
2,3-dimethoxy-5-methyl-1,4-benzoquinone
-
pH 6.0, room temperature
1100 - 1600
2,5-dimethoxy-1,4-benzoquinone
Q8X172
-
1100
2,5-dimethoxy-1,4-benzoquinone
-
pH 6.0, room temperature
2200
2,6-dichlorophenolindophenol
-
pH 6.0, room temperature
1000
2,6-dimethoxy-1,4-benzoquinone
-
pH 6.0, room temperature
1100 - 1600
4,5-dimethoxy-1,2-benzoquinone
Q8X172
-
4000
benzoquinone
-
pH 6.0, room temperature
2600
Fe(CN)63-
-
pH 6.0, room temperature
14.3
ferricyanide
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
11.2
ferrocenium hexafluorophosphate
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
1200
NADH
-
pH 6.0, room temperature
4.95
oxidized 2,6-dichloroindophenol
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
14.6
ubiquinone-1
D9PVS9
cosubstrate: NADPH, pH 7.4, temperature not specified in the publication
15.6
ubiquinone-1
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
6.86
Ubiquinone-10
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
125
1,4-benzoquinone
D9PVS9
cosubstrate: NADPH, pH 7.4, temperature not specified in the publication
315
172
1,4-benzoquinone
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
315
191
ferricyanide
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
132
173
ferrocenium hexafluorophosphate
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
1702
283
oxidized 2,6-dichloroindophenol
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
2451
130
ubiquinone-1
D9PVS9
cosubstrate: NADPH, pH 7.4, temperature not specified in the publication
1150
161
ubiquinone-1
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
1150
204
Ubiquinone-10
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
5628
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.4
3-pyridine aldehyde NADH
halophilic bacterium
-
-
0.7
ADP
-
inhibition of NADH-2,6-dichlorophenolindophenol reductase activity, membrane bound enzyme
1
ADP
-
inhibition of NADH-2,6-dichlorophenolindophenol reductase activity, purified enzyme
0.5
AMP
-
inhibition of NADH-2,6-dichlorophenolindophenol reductase activity, membrane bound enzyme
0.6
AMP
-
inhibition of NADH-2,6-dichlorophenolindophenol reductase activity, purified enzyme
1.5
AMP
halophilic bacterium
-
-
9
ATP
-
inhibition of NADH-2,6-dichlorophenolindophenol reductase activity, membrane bound enzyme
0.0002
Cibacron blue
-
-
0.00022
dicoumarol
-
pH 7.5, 30C
0.0005
dicoumarol
-
-
0.02
NAD+
-
inhibition of-2,6-dichlorophenolindophenol reductase activity, purified enzyme
0.07
NAD+
-
inhibition of NADH-2,6-dichlorophenolindophenol reductase activity, membrane bound enzyme
1.7
NAD+
halophilic bacterium
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00067
1-hydroxy-2-methyl-3-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000403
1-hydroxy-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000217
1-hydroxy-3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000566
1-hydroxy-3-methyl-2-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000213
2-(2',4'-difluorobiphenyl-4-yl)-3-methylquinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000471
2-(2'-fluorobiphenyl-4-yl)-3-methylquinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000355
2-(3-[4-[2-(diethylnitroryl)ethoxy]benzyl]phenyl)-3-methylquinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000719
2-(3-[4-[2-(diethylnitroryl)ethoxy]phenoxy]phenyl)-3-methylquinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000416
2-(4-benzylphenyl)-3-methylquinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.001
2-(4-bromophenyl)-3-methyl-1,8-naphthyridin-4(1H)-one
-
above, pH and temperature not specified in the publication
0.001
2-(4-hydroxyphenyl)quinolin-4(1H)-one
-
above, pH and temperature not specified in the publicationabove
0.001
2-(4-methoxyphenyl)-3-methyl-1,8-naphthyridin-4(1H)-one
-
above, pH and temperature not specified in the publication
0.001
2-(4-methoxyphenyl)quinolin-4(1H)-one
-
above, pH and temperature not specified in the publication
0.001
2-(4-[4-[2-(diethylnitroryl)ethoxy]phenoxy]phenyl)-3-methylquinolin-4(1H)-one
-
above, pH and temperature not specified in the publication
0.000091
2-(hydroxymethyl)-3-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000036
2-methyl-3-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.00001
2-methyl-3-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.00094
2-[3,5-difluoro-4'-(trifluoromethoxy)biphenyl-4-yl]-3-methylquinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000797
2-[4-(4-chlorophenoxy)phenyl]-3-methylquinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000273
2-[4-(4-fluorobenzyl)phenyl]-3-methylquinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000577
2-[4-(4-methoxybenzyl)phenyl]-3-methylquinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000675
2-[4-(trifluoromethoxy)phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000579
2-[4-(trifluoromethyl)phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000251
2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000219
2-[6-(2,4-difluorophenyl)pyridin-3-yl]-3-methylquinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000151
2-[6-(3-fluorophenyl)pyridin-3-yl]-3-methylquinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.001
2-[6-(3-hydroxyphenyl)pyridin-3-yl]-3-methylquinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000556
2-[6-(3-methoxyphenyl)pyridin-3-yl]-3-methylquinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.001
2-[6-(4-hydroxyphenyl)pyridin-3-yl]-3-methylquinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000162
3-(hydroxymethyl)-2-[4'-(trifluoromethoxy)biphenyl-4-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000103
3-(hydroxymethyl)-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.00056
3-chloro-2-(4-methoxyphenyl)quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000513
3-chloro-2-[4-(trifluoromethoxy)phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000328
3-chloro-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000093
3-methoxy-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000096
3-methyl-2-[2'-(trifluoromethyl)biphenyl-4-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000065
3-methyl-2-[3-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000059
3-methyl-2-[4'-(trifluoromethoxy)biphenyl-4-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.001
3-methyl-2-[4-(trifluoromethoxy)phenyl]-1,8-naphthyridin-4(1H)-one
-
above, pH and temperature not specified in the publication
0.001
3-methyl-2-[4-(trifluoromethoxy)phenyl]quinolin-4(1H)-one
-
above, pH and temperature not specified in the publication
0.001
3-methyl-2-[4-(trifluoromethyl)phenyl]-1,8-naphthyridin-4(1H)-one
-
above, pH and temperature not specified in the publication
0.000752
3-methyl-2-[4-(trifluoromethyl)phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.00188
3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]-1,8-naphthyridin-4(1H)-one
-
pH and temperature not specified in the publication
0.000178
3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]-6-(trifluoromethyl)quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000122
3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000092
3-methyl-2-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000109
3-methyl-2-[6-[3-(trifluoromethyl)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.001
3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]-6-(trifluoromethyl)quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.001
3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]-7-(trifluoromethyl)quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000054
3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000102
3-methyl-2-[6-[4-(trifluoromethyl)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.001
3-methyl-6-(methylsulfonyl)-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.001
3-methyl-7-(trifluoromethoxy)-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000797
3-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.001
5-(N-ethyl-N-isopropyl) amiloride
-
IC50: above 0.001 mM, inhibition at 0.1 mM is 30% or less
0.017
5-(N-ethyl-N-isopropyl) amiloride
-
IC50: 0.017 mM
0.063
5-(N-ethyl-N-isopropyl) amiloride
-
IC50: 0.063 mM
0.1
5-(N-ethyl-N-isopropyl) amiloride
-
IC50: above 0.1 mM
0.001
5-(N-methyl-N-isobutyl)amiloride
-
IC50: about 0.001 mM
0.017
5-(N-methyl-N-isobutyl)amiloride
-
IC50: 0.017 mM
0.02
5-(N-methyl-N-isobutyl)amiloride
-
IC50: 0.02 mM
0.045
5-(N-methyl-N-isobutyl)amiloride
-
IC50: 0.045 mM
0.001
5-hydroxy-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.001
5-methoxy-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000626
6,7-difluoro-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000207
6,7-difluoro-3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000344
6,7-difluoro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000286
6,8-difluoro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.001
6-chloro-1-hydroxy-3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000552
6-chloro-2-[3-(4-fluorobenzyl)phenyl]-3-methylquinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.00033
6-chloro-3-(hydroxymethyl)-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000094
6-chloro-3-methyl-2-[3-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000274
6-chloro-3-methyl-2-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000373
6-chloro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.00039
6-chloro-7-fluoro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000191
6-fluoro-3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000075
6-fluoro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000202
6-hydroxy-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000031
7-chloro-3-methyl-2-[3-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.000298
7-chloro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.00039
7-chloro-6-fluoro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.00028
7-hydroxy-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.001
7-methoxy-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.001
8-methoxy-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
pH and temperature not specified in the publication
0.001
benzamil
-
IC50: about 0.001 mM
0.005
benzamil
-
IC50: 0.005 mM
0.042
benzamil
-
IC50: 0.042 mM
0.045
benzamil
-
IC50: 0.045 mM
0.00022
ethyl 2-(4'-chlorobiphenyl-4-yl)-4-oxo-1,4-dihydroquinoline-3-carboxylate
-
pH and temperature not specified in the publication
0.000224
ethyl 2-methyl-3-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4-yl carbonate
-
pH and temperature not specified in the publication
0.0000068
ethyl 3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4-yl carbonate
-
pH and temperature not specified in the publication
0.000406
ethyl 3-methyl-2-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4-yl carbonate
-
pH and temperature not specified in the publication
0.000347
ethyl 4-oxo-2-[4'-(trifluoromethoxy)biphenyl-4-yl]-1,4-dihydroquinoline-3-carboxylate
-
pH and temperature not specified in the publication
0.000987
ethyl 4-oxo-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]-1,4-dihydroquinoline-3-carboxylate
-
pH and temperature not specified in the publication
0.158
flavone
-
IC50: 0.158 mM, weak inhibitor
0.0014
methyl 4-oxo-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]-1,4-dihydroquinoline-3-carboxylate
-
pH and temperature not specified in the publication
0.000092
methyl 6-chloro-4-oxo-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]-1,4-dihydroquinoline-3-carboxylate
-
pH and temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.035
-
soluble fraction with 0.1 mM o-phenanthroline
0.085
-
soluble fraction with 0.1 mM rotenone
0.123
-
soluble fraction with 0.1 mM thenoyltrifluoroacetone
0.682
-
soluble fraction control
7.4
-
partially purified enzyme
376
halophilic bacterium
-
-
3205
-
pH 7.5, 30C
additional information
-
-
additional information
-
-
additional information
-
-
additional information
Q8X172
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.5
-
2 optima: pH 5.5 and pH 7.5
6.5 - 7
-
reaction with 2,6-dichlorophenolindophenol
7.5
-
2 optima: pH 5.5 and pH 7.5
8.5 - 9
-
reaction with 2,6-dichlorophenolindophenol
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.2
-
isoelectric focusing
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Methanothermobacter marburgensis DSM 2133
-
-
-
Manually annotated by BRENDA team
-
the Nqo11 subunit has three transmembrane segments and its C-terminus protrudes into the cytoplasmic phase
Manually annotated by BRENDA team
P50939, P50973, P50974
transmembrane topology of the NuoL, M and N subunits
Manually annotated by BRENDA team
Q8X172
100000 g supernatant of a day 7 mycelial extract
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37000
-
soluble enzyme, gel filtration
392808
46000
-
SDS-PAGE
673551
50000
Q8X172
gel filtration
392833
50000
-
gel filtration
394359
50000
-
SDS-PAGE
676787
55000
-
SDS-PAGE
672657
64000
halophilic bacterium
-
gel filtration
392806
79000
-
gel filtration
392815
97000
-
membrane-bound enzyme, gel filtration
392809
138000
-
gel filtration
392813
190000
-
MALDI-TOF MS, NDH-1S complex
671838
215000
-
gel filtration
392818
300000
-
MALDI-TOF MS, NDH-1M complex
671838
450000
-
MALDI-TOF MS, NDH-1L complex
671838
490000
-
MALDI-TOF MS, NDH-1MS complex
671838
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 38000, SDS-PAGE
?
-
x * 57000, SDS-PAGE
?
-
x * 22000, SDS-PAGE
dimer
D9PVS9
2 * 24000, SDS-PAGE
dimer
-
2 * 65000, SDS-PAGE
dimer
Q8X172
2 * 22000, SDS-PAGE
dimer
-
2 * 25000, SDS-PAGE
dimer
D9PVS9
the crystal structure of the enzyme features a homodimeric enzyme where each monomer adopts the flavodoxin-like alpha/beta fold
dimer
Methanothermobacter marburgensis DSM 2133
-
2 * 24000, SDS-PAGE, the crystal structure of the enzyme features a homodimeric enzyme where each monomer adopts the flavodoxin-like alpha/beta fold
-
hexamer
-
6 * 15500, membrane-bound enzyme, SDS-PAGE
monomer
-
1 * 50000, SDS-PAGE
monomer
-
1 * 38000, soluble enzyme, SDS-PAGE
monomer
-
1 * 52000
monomer
Rhodobacter capsulatus 37b4
-
1 * 38000, soluble enzyme, SDS-PAGE
-
additional information
-
ND5 subunit is involved in H+ (Na+) translocation
additional information
-
the membrane domain Nqo10 subunit consists of five transmembrane segments with the N- and C-terminal regions facing the periplasmic and cytoplasmic sides of the membrane. Topological model for the Nqo10 subunit
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sitting-drop vapor diffusion, Structures of WrbA in complex with benzoquinone and NADH suggest a sequential binding mechanism for both molecules in the catalytic cycle
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
-
5 min, in presence of FMN, riboflavin, and NADH, stable
392808
40
-
40 min, 90% loss of the activity with ferricyanide
392818
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
activated complex prepared with diphosphatidylglycerol is stable, activated complex prepared with phosphatidylglycerol loses activity rapidly
-
in presence of 100 mM NaCl, immediate and exponential loss of activity. In presence of 250 mM NaCl stable for about 30 min before the loss of activity becomes exponential. In presence of 500 mM NaCl the enzyme is stable during 150 min
halophilic bacterium
-
NADH enhances stability
halophilic bacterium
-
stable to repeated freezing and thawing
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, phosphate buffer, pH 6, loss of activity within several days
Q8X172
-20C, sodium citrate buffer, pH 6, 20% v/v ethylene glycol, stable
Q8X172
-20C, sodium citrate buffer, pH 6, loss of activity within several days
Q8X172
4C, 1 month, less than 10% loss of activity
halophilic bacterium
-
-25C, stable for several months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
nickel affinity chromatography
-
using 1% Triton X-100 treatment, ammonium sulfate precipitation, sucrose density gradient and column chromatography on DEAE-cellulose
-
DEAE-Toyopearl column chromatography and Heparin-Sepharose column chromatography
-
Talon resin column chromatography
-
using column chromatography on Phenyl-Sepharose, hydroxyapatite, DEAE-Sepharose and Superdex 200
Q8X172
-
halophilic bacterium
-
Talon resin column chromatography
-
using ammonium sulfate treatment, pH adjustment to 5, centrifugation, adjustement of the supernatant solution to pH 6, protamine-CHCl3 treatment, column chromatography on DEAE-Sephadex and second ammonium sulfate treatment
-
partial, using dialysis and column chromatography on DEAE-Sepharose and Phenyl-Sepharose
-
Ni2+ column chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
expression of the NuoG subunit in Escherichia coli
-
characterization of the gene that encodes the enzyme, the gene has substancial similarity to NAD(P)H:quinone reductase genes from other fungi
Q8X172
expressed in Escherichia coli NADH dehydrogenase knockout strain ANN0222
-
overexpression of membrane domain Nqo10 subunit in Escherichia coli
-
overexpression of the Nqo11 subunit in membrane section of Escherichia coli using the maltose-binding protein fusion system
-
NDH2, expression in Escherichia coli strain F571
-
the Nqo3 fragment containing the CXXCXXXCX27C motif is heterologously overexpressed, using a glutathione S-transferase fusion system
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
2-alkyl-1,4-quinone structure with C-3 unsubstituted or substituted with Br is critical as a common inductive signal by quinone derivatives. Slight inductive activity is shown by 2-methylene-4-butyrolactone, methylacrylate and methyl vinyl ketone
-
induced up to 23fold by growth in presence of menadione
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D135A
-
NuoM-subunit mutant, deamino-NADH oxidase activity is 44% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 44% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 80% of wild-type activity
D135E
-
NuoM-subunit mutant, deamino-NADH oxidase activity is 87% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 91% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 93% of wild-type activity
D135N
-
NuoM-subunit mutant, deamino-NADH oxidase activity is 78% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 78% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 86% of wild-type activity
D79A
-
mutation partially suppresses deamino-NADH-quinone reductase activity. In terms of immunochemical and NADH dehydrogenase activity-staining analysis the mutant is similar to the wild-type enzyme suggesting that the mutation does not significantly affect the assembly of the peripheral subunits in situ
D79N
-
mutation partially suppresses deamino-NADH-quinone reductase activity. In terms of immunochemical and NADH dehydrogenase activity-staining analysis the mutant is similar to the wild-type enzyme suggesting that the mutation does not significantly affect the assembly of the peripheral subunits in situ
D79N/E81Q
-
almost complete loss of energy-transducing activities, does not display any loss of deamino-NADH-K3Fe(CN)6 reductase activity
D84A
-
NuoM-subunit mutant, deamino-NADH oxidase activity is 83% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 94% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 92% of wild-type activity
D84N
-
NuoM-subunit mutant, deamino-NADH oxidase activity is 89% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 83% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 97% of wild-type activity
E144A
-
NuoM-subunit mutant, deamino-NADH oxidase activity is 2% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 10% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 103% of wild-type activity
E144D
-
NuoM-subunit mutant, deamino-NADH oxidase activity is 89% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 97% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 100% of wild-type activity
E144Q
-
NuoM-subunit mutant, deamino-NADH oxidase activity is 2% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 2% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 98% of wild-type activity
E51A
-
mutation partially suppresses deamino-NADH-quinone reductase activity. In terms of immunochemical and NADH dehydrogenase activity-staining analysis the mutant is similar to the wild-type enzyme suggesting that the mutation does not significantly affect the assembly of the peripheral subunits in situ
E81A
-
mutation partially suppresses deamino-NADH-quinone reductase activity. In terms of immunochemical and NADH dehydrogenase activity-staining analysis the mutant is similar to the wild-type enzyme suggesting that the mutation does not significantly affect the assembly of the peripheral subunits in situ
E81Q
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mutation partially suppresses deamino-NADH-quinone reductase activity
H196A
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NuoM-subunit mutant, deamino-NADH oxidase activity is 79% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 87% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 99% of wild-type activity
H241A
-
NuoM-subunit mutant, deamino-NADH oxidase activity is 88% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 87% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 90% of wild-type activity
H322A
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NuoM-subunit mutant, deamino-NADH oxidase activity is 100% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 94% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 99% of wild-type activity
H348A
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NuoM-subunit mutant, deamino-NADH oxidase activity is 92% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 98% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 100% of wild-type activity
K234A
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NuoM-subunit mutant, deamino-NADH oxidase activity is 8% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 16% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 91% of wild-type activity
K265A
-
NuoM-subunit mutant, deamino-NADH oxidase activity is 35% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 30% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 96% of wild-type activity
K46A
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mutation scarcely inhibits deamino-NADH-quinone reductase activity. In terms of immunochemical and NADH dehydrogenase activity-staining analysis the mutant is similar to the wild-type enzyme suggesting that the mutation does not significantly affect the assembly of the peripheral subunits in situ
P245A
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NuoM-subunit mutant, deamino-NADH oxidase activity is 102% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 99% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 100% of wild-type activity
R273A
-
NuoM-subunit mutant, deamino-NADH oxidase activity is 92% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 97% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 105% of wild-type activity
R365A
-
NuoM-subunit mutant, deamino-NADH oxidase activity is 87% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 85% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 92% of wild-type activity
R369H
-
NuoM-subunit mutant, deamino-NADH oxidase activity is 63% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 68% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 94% of wild-type activity
W243A
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NuoM-subunit mutant, deamino-NADH oxidase activity is 103% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 98% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 97% of wild-type activity
W243Y
-
NuoM-subunit mutant, deamino-NADH oxidase activity is 104% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 104% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 108% of wild-type activity
Y435A
-
NuoM-subunit mutant, deamino-NADH oxidase activity is 99% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 102% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 94% of wild-type activity
A49C
-
Escherichia coli cells expression the Nqo10 mutant protein grow normally. The expression level and the subcellular distribution of the mutated MBP-fused Nqo10 subunit shows no substantial difference from those of the wild-type
E50C
-
Escherichia coli cells expression the Nqo10 mutant protein grow normally. The expression level and the subcellular distribution of the mutated MBP-fused Nqo10 subunit shows no substantial difference from those of the wild-type
G84C
-
Escherichia coli cells expression the Nqo10 mutant protein grow normally. The expression level and the subcellular distribution of the mutated MBP-fused Nqo10 subunit shows no substantial difference from those of the wild-type
H168C
-
Escherichia coli cells expression the Nqo10 mutant protein grow normally. The expression level and the subcellular distribution of the mutated MBP-fused Nqo10 subunit shows no substantial difference from those of the wild-type
M2C
-
Escherichia coli cells expression the Nqo10 mutant protein grow normally. The expression level and the subcellular distribution of the mutated MBP-fused Nqo10 subunit shows no substantial difference from those of the wild-type
N25C
-
Escherichia coli cells expression the Nqo10 mutant protein grow normally. The expression level and the subcellular distribution of the mutated MBP-fused Nqo10 subunit shows no substantial difference from those of the wild-type
R142C
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Escherichia coli cells expression the Nqo10 mutant protein grow normally. The expression level and the subcellular distribution of the mutated MBP-fused Nqo10 subunit shows no substantial difference from those of the wild-type
S114C
-
Escherichia coli cells expression the Nqo10 mutant protein grow normally. The expression level and the subcellular distribution of the mutated MBP-fused Nqo10 subunit shows no substantial difference from those of the wild-type
W182C
-
Escherichia coli cells expression the Nqo10 mutant protein grow normally. The expression level and the subcellular distribution of the mutated MBP-fused Nqo10 subunit shows no substantial difference from those of the wild-type
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
analysis
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addition of the type 2 NADH:quinone oxidoreductase complex along with NADH to enzyme assay of methane monooxygenase with duroquinol increases the activity of purified preparations nby maintaining a high duroquinol/duroquinone ratio
analysis
Methylococcus capsulatus Bath
-
addition of the type 2 NADH:quinone oxidoreductase complex along with NADH to enzyme assay of methane monooxygenase with duroquinol increases the activity of purified preparations nby maintaining a high duroquinol/duroquinone ratio
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medicine
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key target in anti-tuberculosis therapy