Information on EC 1.5.99.12 - cytokinin dehydrogenase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
1.5.99.12
-
RECOMMENDED NAME
GeneOntology No.
cytokinin dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
N6-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor
show the reaction diagram
-
-
-
-
N6-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor
show the reaction diagram
reaction scheme and mechanism, reduction of N6-(2-isopentenyl)adenine is rapidly performed, the reaction with O2 is slowly
Q9T0N8
N6-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor
show the reaction diagram
reaction scheme and mechanism, the substrate displays a plug-into-socket binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin, bipartite architecture of the catalytic centre, which consists of a funnel-shaped region on the protein surface and an internal cavity lined by the flavin ring, the 3 active site regions are connected by a pore of about 4 A diameter
-
N6-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor
show the reaction diagram
reaction might also be catalyzed by a different enzyme in Dictyostelium discoideum, since no related sequence has been found
-
N6-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor
show the reaction diagram
Asp162 and Glu275 involved in substrate binding
Q9FUJ1
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Zeatin biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
N6-dimethylallyladenine:acceptor oxidoreductase
A flavoprotein(FAD). Catalyses the oxidation of cytokinins, a family of N6-substituted adenine derivatives that are plant hormones, where the substituent is a dimethylallyl or other prenyl group. Although this activity was previously thought to be catalysed by a hydrogen-peroxide-forming oxidase, this enzyme does not require oxygen for activity and does not form hydrogen peroxide. 2,6-Dichloroindophenol, methylene blue, nitroblue tetrazolium, phenazine methosulfate and Cu(II) in the presence of imidazole can act as acceptors. This enzyme plays a part in regulating rice-grain production, with lower levels of the enzyme resulting in enhanced grain production [2].
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
AtCKX2
-
seven distinct genes AtCKX1 to AtCKX7, AtCKX2 is subject of the study
AtCKX2
Q9FUJ3
-
AtCKX3
Q9LTS3
-
CKO
Triticum durum Bezenchukskaya 139
-
-
-
CKO
-
-
CKO1
-
-
CKX
Q84U27
-
CKX
Q8H6F6
-
CKX
Nostoc sp., Oryza sativa, Phaseolus sp.
-
-
CKX
Solanum lycopersicum Improved Pope
-
-
-
CKX
D3JAJ5
-
CKX
Triticum aestivum Bashkirskaya 24
-
-
-
CKX
Triticum aestivum Yanyou 361
D3JAJ5
-
-
CKX1
Q94KI4
-
CKX1
-
-
CKX3
D3JAJ5
-
CKX3
Triticum aestivum Yanyou 361
D3JAJ5
-
-
cytokinin dehydrogenase
-
-
cytokinin dehydrogenase CKX1
Q9T0N8
-
cytokinin dehydrogenase CKX10
B6V8F7
-
cytokinin oxidase
-
-
-
-
cytokinin oxidase
-
-
cytokinin oxidase
Q9T0N8
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
Q9FUJ3
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
Q9FE45
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
Q8H6F6
-
cytokinin oxidase/dehydrogenase
Q94KI4
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
Nostoc sp., Oryza sativa, Phaseolus sp.
-
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
A0MQ40
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
Rhipsalidopsis sp.
-
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
Schlumbergera sp.
-
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
D3JAJ5
-
cytokinin oxidase/dehydrogenase
Q94KI5
-
cytokinin oxidase/dehydrogenase
Triticum aestivum Bashkirskaya 24
-
-
-
cytokinin oxidase/dehydrogenase
Triticum aestivum Yanyou 361
D3JAJ5
-
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
Q9T0N8
-
HvCKX1
-
-
N6-(D2-isopentenyl)adenosine oxidase
-
-
-
-
N6-isopentenylaldenine oxidase
-
-
-
-
OsCKX2
-
-
PsCKX1
A0MQ40
-
PsCKX2
A0MQ40
-
zeatin oxidase
-
-
-
-
ZmCKO1
-
-
ZmCKO1
Q9T0N8
-
ZmCKX1
-
-
isopentenyladenosine oxidase
-
-
-
-
additional information
Q9FUJ3
formerly EC 1.4.3.18
additional information
-
formerly EC 1.4.3.18
additional information
-
see also EC 1.4.3.18
additional information
-
formerly EC 1.4.3.18
additional information
-
enzyme belongs to the CKX gene family
additional information
Q9T0N8
enzyme belongs to the plant CKX family
CAS REGISTRY NUMBER
COMMENTARY
55326-39-1
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
isozyme AtCKX1
UniProt
Manually annotated by BRENDA team
isozyme AtCKX3
UniProt
Manually annotated by BRENDA team
isozyme AtCKX7
-
-
Manually annotated by BRENDA team
isozymes AtCKX1, AtCKX4, and AtCKX7
-
-
Manually annotated by BRENDA team
L., Heynh. variety Columbia
-
-
Manually annotated by BRENDA team
cv. Cocozelle
-
-
Manually annotated by BRENDA team
gene DsCKX1 or DSCKX1
SwissProt
Manually annotated by BRENDA team
cultivar Alexis
-
-
Manually annotated by BRENDA team
cultivars Golden Promise, Scarlett, Torka, and Kontesa
SwissProt
Manually annotated by BRENDA team
cv Amulet
-
-
Manually annotated by BRENDA team
cv. Luxor, gene HvCKX1, barley contains at least 7 different gene homologues
SwissProt
Manually annotated by BRENDA team
cv. Luxor, gene HvCKX2, barley contains at least 7 different gene homologues
SwissProt
Manually annotated by BRENDA team
cv. Luxor, gene HvCKX3, barley contains at least 7 different gene homologues
SwissProt
Manually annotated by BRENDA team
cv. Luxor, gene HvCKX7, genomic DNA, barley contains at least 7 different gene homologues, gene HvCKX7, genomic DNA
-
-
Manually annotated by BRENDA team
gene and isozyme HvCKX1
-
-
Manually annotated by BRENDA team
gene HvCKX2
SwissProt
Manually annotated by BRENDA team
L., cv. Alexis
-
-
Manually annotated by BRENDA team
2 isozymes: 1 glycosylated, 1 non-glycosylated
-
-
Manually annotated by BRENDA team
cv Wisconsin 38
-
-
Manually annotated by BRENDA team
cv. Wisconsin 38, wild-type, and transgenic tobacco conditionally overexpressing isopentenyltransferase IPT
-
-
Manually annotated by BRENDA team
L. cv. Wisconsin 38
-
-
Manually annotated by BRENDA team
no activity in Physcomitrella patens
-
-
-
Manually annotated by BRENDA team
no activity in Pichia pastoris
-
-
-
Manually annotated by BRENDA team
no activity in Prochlorococcus marinus
-
-
-
Manually annotated by BRENDA team
no activity in Raphanus sativus
-
-
-
Manually annotated by BRENDA team
no activity in Saccharomyces cerevisiae
-
-
-
Manually annotated by BRENDA team
no activity in Synechocystis sp.
-
-
-
Manually annotated by BRENDA team
no activity in Synechocystis sp. PCC 6803
-
-
-
Manually annotated by BRENDA team
i.e. Anabaena sp., PCC 7120, gene NsCKX1
-
-
Manually annotated by BRENDA team
5 distinct genes OsCKX1-OsCKX5
-
-
Manually annotated by BRENDA team
isozymes OsCKX1, OsCKX4, and OsCKX11
-
-
Manually annotated by BRENDA team
Phaseolus sp.
2 isozymes: 1 glycosylated, 1 non-glycosylated
-
-
Manually annotated by BRENDA team
cv Great Northern
-
-
Manually annotated by BRENDA team
Hedw., wild type and transgenic cells expressing the AtCKX2 gene from Arabidopsis thaliana, expression of AtCKX2 results in a significant decrease of cytokinin level and morphological changes such as altered morphology of filaments, impaired budding, and loss of sexual reproductivity
-
-
Manually annotated by BRENDA team
highest activity in one year old plants
-
-
Manually annotated by BRENDA team
cv. Scinado and cv. Manuela
-
-
Manually annotated by BRENDA team
L. cv. Gotik
-
-
Manually annotated by BRENDA team
L. cv. Scinado and cv. Manuela, cv. Scinado normally requires shorter growing period than cv. Manuela
UniProt
Manually annotated by BRENDA team
PsCKX1; L. cv. Sparkle, wild type and R50 carrying a recessive sym16 which is a pleiotropic mutant of pea which develops few, pale nodules and has pale young leaves, R50 has reduced CKX activity and increased cytokinin level compared with wild type, sequencing of DNA from both strains did not show any obvious mutations within the gene
UniProt
Manually annotated by BRENDA team
radish, cv. Rampouch
-
-
Manually annotated by BRENDA team
Rhipsalidopsis sp.
-
-
-
Manually annotated by BRENDA team
phytopathogen causing the witches broom disease, gene FasV of RfCKX1
-
-
Manually annotated by BRENDA team
Schlumbergera sp.
-
-
-
Manually annotated by BRENDA team
Solanum lycopersicum Improved Pope
-
-
-
Manually annotated by BRENDA team
CKX3; gene TaCKX3
UniProt
Manually annotated by BRENDA team
cultivar Wanad
-
-
Manually annotated by BRENDA team
cv Samantha
-
-
Manually annotated by BRENDA team
cv. Samantha, gene TaCKX1, wheat contains at least 8 different gene homologues
SwissProt
Manually annotated by BRENDA team
Triticum aestivum Bashkirskaya 24
-
-
-
Manually annotated by BRENDA team
Triticum aestivum Yanyou 361
CKX3; gene TaCKX3
UniProt
Manually annotated by BRENDA team
Triticum durum Bezenchukskaya 139
-
-
-
Manually annotated by BRENDA team
cv. Cellux 225
SwissProt
Manually annotated by BRENDA team
enzyme and gene ZmCKX1
SwissProt
Manually annotated by BRENDA team
enzyme ZmCKO1
-
-
Manually annotated by BRENDA team
gene ZmCKX1
SwissProt
Manually annotated by BRENDA team
isoyzmes ZmCKX1, ZmCKX2, and ZmCKX10
-
-
Manually annotated by BRENDA team
variety Cellux 225
-
-
Manually annotated by BRENDA team
ZmCKX1
SwissProt
Manually annotated by BRENDA team
Zea mays ZmCKX1
ZmCKX1
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
evolution
-, O22213, Q9LTS3
the CKX gene family of Arabidopsis thaliana comprises seven members; the CKX gene family of Arabidopsis thaliana comprises seven members; the CKX gene family of Arabidopsis thaliana comprises seven members
malfunction
Q94KI4
silencing of the CKX1 gene decreases the cytokinin oxidase/dehydrogenase level in barley and leads to higher plant productivity (number of seeds per plant) and root weight
malfunction
-
silencing of the CKX1 gene decreases the cytokinin oxidase/dehydrogenase level in barley and leads to higher plant productivity (number of seeds per plant) and root weight
malfunction
-
overexpression of the AtCKX genes in tobacco leads to shoot senescence, an increase of the root meristem zone, and enhanced formation of lateral roots
malfunction
-
silencing of the HvCKX1 gene of barley cytokinin oxidase leads to a significant inhibition of cytokinin oxidase activity accompanied by an increase in productivity of this culture
malfunction
-
chlorotic leaves associated with a reduction in representative Ccytokinin levels and high H2O2 concentrations under drought, salt and iron-deficiency stresses of tomato plants.Chlorosis-inducing stresses (salt and iron-deficiency) induce lower chlorophyll levels as compared to drought stress and control, overview
malfunction
-
inhibition of the enzyme by 24-epibrassinolide leads to activation of production of cytokinins in roots and shoots, of which excessive amounts are transferred into the storage forms
malfunction
Solanum lycopersicum Improved Pope
-
chlorotic leaves associated with a reduction in representative Ccytokinin levels and high H2O2 concentrations under drought, salt and iron-deficiency stresses of tomato plants.Chlorosis-inducing stresses (salt and iron-deficiency) induce lower chlorophyll levels as compared to drought stress and control, overview
-
malfunction
Triticum aestivum Bashkirskaya 24
-
inhibition of the enzyme by 24-epibrassinolide leads to activation of production of cytokinins in roots and shoots, of which excessive amounts are transferred into the storage forms
-
metabolism
-
comparison of the functional spectra of cytokinins and brassinosteroids, overview
metabolism
Triticum aestivum Bashkirskaya 24
-
comparison of the functional spectra of cytokinins and brassinosteroids, overview
-
physiological function
-
cytokinin oxidase/dehydrogenase irreversibly degrades the plant hormones cytokinins and thereby participates in their homeostasis
physiological function
-
cytokinins are plant growth regulators playing crucial role in almost all the developmental processes. Oxidative breakdown of cytokinins by cytokinin oxidase is an important process by which plant tissues control the levels and distribution of cytokinins
physiological function
-
the enzyme catalyzes the irreversible degradation of cytokinin phytohormones that are extremely necessary for growth, development, and differentiation of plants. Cytokinin oxidase plays an important role in the regulation of levels of cytokinin hormones, the enzyme directly influences cytokinin-regulated physiological processes in plants
physiological function
-
cytokinin dehydrogenase catalyzes the irreversible degradation of cytokinins, that play a role in the regulation of hydrogen peroxide accumulation while H2O2 is involved in chlorophyll degradation. CKX differentially regulates representative cytokinin levels and indirectly influence H2O2 accumulation in tomato leaves.mechanism, overview. The CKX isoforms in the tomato leaf appear to have distinct roles in differentially regulating CK levels and indirectly influencing H2O2 accumulation
physiological function
D3JAJ5
cytokinin oxidase/dehydrogenase plays an important role in regulating plant growth and development
physiological function
-
cytokinin oxidase is involved in the regulation of cytokinin content by 24-epibrassinolide in wheat seedlings
physiological function
-
the regulation of CKO activity might be one of the important mechanisms determining plant response to treatments via the changes in the cytokinin concentration. The presence of apoplastic (xylem) cytokinins located outside the cells may be important because the receptors of cytokinins initiating activation of CKO gene expression are located in the cell membrane
physiological function
Solanum lycopersicum Improved Pope
-
cytokinin dehydrogenase catalyzes the irreversible degradation of cytokinins, that play a role in the regulation of hydrogen peroxide accumulation while H2O2 is involved in chlorophyll degradation. CKX differentially regulates representative cytokinin levels and indirectly influence H2O2 accumulation in tomato leaves.mechanism, overview. The CKX isoforms in the tomato leaf appear to have distinct roles in differentially regulating CK levels and indirectly influencing H2O2 accumulation
-
physiological function
Triticum aestivum Bashkirskaya 24
-
cytokinin oxidase is involved in the regulation of cytokinin content by 24-epibrassinolide in wheat seedlings
-
physiological function
Triticum aestivum Yanyou 361
-
cytokinin oxidase/dehydrogenase plays an important role in regulating plant growth and development
-
physiological function
Triticum durum Bezenchukskaya 139
-
the regulation of CKO activity might be one of the important mechanisms determining plant response to treatments via the changes in the cytokinin concentration. The presence of apoplastic (xylem) cytokinins located outside the cells may be important because the receptors of cytokinins initiating activation of CKO gene expression are located in the cell membrane
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
cis-zeatin + acceptor + H2O
?
show the reaction diagram
-
-, preferred substrate of isozyme ZmCKX10
-
-
?
cis-zeatin + electron acceptor
(2Z)-4-hydroxy-3-methylbut-2-enal + adenine + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
cis-zeatin + electron acceptor
(2Z)-4-hydroxy-3-methylbut-2-enal + adenine + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
cis-zeatin + oxidized 2,6-dichlorophenolindophenol
?
show the reaction diagram
-
-
-
-
?
cis-zeatin + oxidized 2,6-dichlorophenolindophenol
?
show the reaction diagram
B6V8F7, Q9T0N8
-
-
-
?
cis/trans-zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
Q9T0N8
-, enzyme catalyzes the irreversible degradation of cytokinins
-
-
ir
cis/trans-zeatin riboside + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
Q9T0N8
-
-
-
ir
cytokinin + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
? + reduced 2,3-dimethoxy-5-methyl-1,4-benzoquinone
show the reaction diagram
-, Q84U27, Q8H6F6, Q94KI4
-
-
-
?
cytokinin riboside + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
? + reduced 2,3-dimethoxy-5-methyl-1,4-benzoquinone
show the reaction diagram
-, Q84U27, Q8H6F6, Q94KI4
-
-
-
?
dihydrozeatin + electron acceptor
?
show the reaction diagram
-
-
-
-
?
isopentenyladenine + 2,6-dichlorophenolindophenol
adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
isopentenyladenine + 2,6-dichlorophenolindophenol
adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-, Q84U27, Q8H6F6, Q94KI4
-
-
-
?
isopentenyladenine 9-glucoside + 2,6-dichlorophenolindophenol
adenine 9-glucoside + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-, Q84U27, Q8H6F6, Q94KI4
-
-
-
?
isopentenyladenosine + 2,6-dichlorophenolindophenol
adenosine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-, Q84U27, Q8H6F6, Q94KI4
-
-
-
?
isopentenyladenosine + acceptor + H2O
? + reduced acceptor
show the reaction diagram
Triticum aestivum, Triticum aestivum Bashkirskaya 24
-
-
-
-
?
isopentenyladenosine monophosphate + 2,6-dichlorophenolindophenol
adenosine monophosphate + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-, Q84U27, Q8H6F6, Q94KI4
-
-
-
?
kinetin + 2,3-dimethoxy-5-methyl-1,4-benzoquinone + H2O
?
show the reaction diagram
-, O22213, Q9LTS3
-
-
-
?
kinetin + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
low activity
-
-
?
kinetin + electron acceptor
2-furaldehyde + adenine + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
kinetin + FAD
2-furaldehyde + adenine + FADH2
show the reaction diagram
-
-
-
-
?
kinetin riboside + 2,3-dimethoxy-5-methyl-1,4-benzoquinone + H2O
?
show the reaction diagram
-, O22213, Q9LTS3
-
-
-
?
N-benzyladenine + electron acceptor
benzaldehyde + adenine + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
N-methyl-isopentenyladenine + FAD
?
show the reaction diagram
-
-
-
-
?
N6-(2-butynyl)adenine + 2,6-dichlorophenol indophenol
?
show the reaction diagram
-
very effective electron acceptor
-
-
?
N6-(2-isopentenyl)adenine + 1,4-naphthoquinone
adenine + 3-methylbut-2-enal + reduced 1,4-naphthoquinone
show the reaction diagram
Q9T0N8
-
-
-
?
N6-(2-isopentenyl)adenine + 1,4-naphthoquinone
?
show the reaction diagram
-
very effective electron acceptor
-
-
?
N6-(2-isopentenyl)adenine + 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical + H2O
adenine + 3-methylbut-2-enal + ?
show the reaction diagram
-
-
-
-
?
N6-(2-isopentenyl)adenine + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
adenine + 3-methylbut-2-enal + reduced 2,3-dimethoxy-5-methyl-1,4-benzoquinone
show the reaction diagram
Q9T0N8
i.e. coenzyme Q0
-
-
?
N6-(2-isopentenyl)adenine + 2,3-dimethoxy-5-methyl-1,4-benzoquinone + H2O
adenine + 3-methylbut-2-enal + 2,3-dimethoxy-5-methyl-1,4-benzoquinol
show the reaction diagram
-
4-nitrosoresorcinol-1-monomethyl ether serves as a weak electron acceptor of CKX
-
-
?
N6-(2-isopentenyl)adenine + 2,3-dimethoxy-5-methyl-1,4-benzoquinone + H2O
adenine + 3-methylbut-2-enal + ?
show the reaction diagram
-, O22213, Q9LTS3
-
-
-
?
N6-(2-isopentenyl)adenine + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
-
-
-
?
N6-(2-isopentenyl)adenine + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
-
-
-
?
N6-(2-isopentenyl)adenine + 2,6-dichlorophenol indophenol
?
show the reaction diagram
A0MQ40
-
-
-
?
N6-(2-isopentenyl)adenine + 2,6-dichlorophenol indophenol
?
show the reaction diagram
-
very effective electron acceptor
-
-
?
N6-(2-isopentenyl)adenine + 2,6-dichlorophenol indophenol
?
show the reaction diagram
-
very effective electron acceptor
-
-
?
N6-(2-isopentenyl)adenine + 2,6-dichlorophenol indophenol
?
show the reaction diagram
-
very effective electron acceptor
-
-
?
N6-(2-isopentenyl)adenine + 2,6-dichlorophenolindophenol
adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
N6-(2-isopentenyl)adenine + 2,6-dichlorophenolindophenol
adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
Q9T0N8
-
-
-
?
N6-(2-isopentenyl)adenine + 2,6-dichlorophenolindophenol + H2O
adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
N6-(2-isopentenyl)adenine + 2,6-dichlorophenolindophenol + H2O
adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
N6-(2-isopentenyl)adenine + 2,6-dichlorophenolindophenol + H2O
adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-, Q94KI4
-
-
-
?
N6-(2-isopentenyl)adenine + 4-nitrosoresorcinol-1-monomethyl ether + H2O
adenine + 3-methylbut-2-enal + reduced 4-nitrosoresorcinol-1-monomethyl ether
show the reaction diagram
-
4-nitrosoresorcinol-1-monomethyl ether serves as a weak electron acceptor of CKX
-
-
?
N6-(2-isopentenyl)adenine + acceptor + H2O
?
show the reaction diagram
-
preferred substrate of isozyme AtCKX4
-
-
?
N6-(2-isopentenyl)adenine + caffeic acid
adenine + 3-methylbut-2-enal + ?
show the reaction diagram
Q9T0N8
-
-
-
?
N6-(2-isopentenyl)adenine + coenzyme Q1
adenine + 3-methylbut-2-enal + reduced coenzyme Q1
show the reaction diagram
Q9T0N8
-
-
-
?
N6-(2-isopentenyl)adenine + CuCl2
?
show the reaction diagram
-
-
-
-
?
N6-(2-isopentenyl)adenine + CuCl2
?
show the reaction diagram
-
low activity
-
-
?
N6-(2-isopentenyl)adenine + CuCl2 + H2O
adenine + 3-methylbut-2-enal + ?
show the reaction diagram
-
-
-
-
?
N6-(2-isopentenyl)adenine + cytochrome c
adenine + 3-methylbut-2-enal + reduced cytochrome c
show the reaction diagram
Q9T0N8
-
-
-
?
N6-(2-isopentenyl)adenine + daphnoretin
adenine + 3-methylbut-2-enal + ?
show the reaction diagram
Q9T0N8
-
-
-
?
N6-(2-isopentenyl)adenine + duroquinone
?
show the reaction diagram
-
low activity
-
-
?
N6-(2-isopentenyl)adenine + electron acceptor
adenine + 3-methylbut-2-enal + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
N6-(2-isopentenyl)adenine + FAD
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
Q9T0N8
-, the natural terminal electron acceptor probably is a p-quinone or a similar compound, high substrate specificity for N6-(2-isopentenyl)adenine
-
-
?
N6-(2-isopentenyl)adenine + FAD
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
-
formation of a binary enzyme-product complex between the cytokinin imine and the reduced enzyme. The binary complex of the reduced enzyme and imine product intermediate decays relatively slowly to form an unbound product, cytokinin imine, which accumulates in the reaction mixture
-
-
?
N6-(2-isopentenyl)adenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
-
-
-
-
ir
N6-(2-isopentenyl)adenine + hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one + H2O
adenine + 3-methylbut-2-enal + reduced hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one
show the reaction diagram
-
CKX1 is able to use hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one as a poor electron acceptor at neutral pH levels, but not in acidic pH levels
-
-
?
N6-(2-isopentenyl)adenine + o-coumaric acid
adenine + 3-methylbut-2-enal + ?
show the reaction diagram
Q9T0N8
-
-
-
?
N6-(2-isopentenyl)adenine + O2
adenine + 3-methylbut-2-enal + H2O2
show the reaction diagram
Q9T0N8
oxidative degradation of cytokinins, slow reaction, low substrate specificity
-
-
?
N6-(2-isopentenyl)adenine + O2
?
show the reaction diagram
-
low activity
-
-
?
N6-(2-isopentenyl)adenine + plantacyanine
adenine + 3-methylbut-2-enal + reduced plantacyanine
show the reaction diagram
Q9T0N8
electron acceptor from spinach
-
-
?
N6-(2-isopentenyl)adenine + potassium ferricyanide
?
show the reaction diagram
-
low activity
-
-
?
N6-(2-isopentenyl)adenine + rosmarinic acid
adenine + 3-methylbut-2-enal + ?
show the reaction diagram
Q9T0N8
-
-
-
?
N6-(2-isopentenyl)adenine + scopoletin
adenine + 3-methylbut-2-enal + ?
show the reaction diagram
Q9T0N8
-
-
-
?
N6-(2-isopentenyl)adenine + vitamin K1
adenine + 3-methylbut-2-enal + reduced vitamin K1
show the reaction diagram
Q9T0N8
-
-
-
?
N6-(2-isopentenyl)adenine + vitamin K3
adenine + 3-methylbut-2-enal + reduced vitamin K3
show the reaction diagram
Q9T0N8
-
-
-
?
N6-(2-isopentenyl)adenine + vitamin K3
?
show the reaction diagram
-
low activity
-
-
?
N6-(2-isopentenyl)adenine 9-beta-D-glucoside + 3',5'-dimethoxy-4-hydroxyacetophenone + H2O
adenine 9-beta-D-glucoside + 3-methylbut-2-enal + ?
show the reaction diagram
-, O22213, Q9LTS3
i.e. iP9G, best substrate for isozyme AtCKX1 at pH 5.0, 3',5'-dimethoxy-4-hydroxyacetophenone is the best acceptor
-
-
?
N6-(2-isopentenyl)adenine 9-beta-D-riboside + 2,3,5-triphenyl-tetrazolium chloride + H2O
adenine 9-beta-D-riboside + 3-methylbut-2-enal + ?
show the reaction diagram
-, O22213, Q9LTS3
-
-
-
?
N6-(2-isopentenyl)adenine 9-beta-D-riboside + 2,3-dimethoxy-5-methyl-1,4-benzoquinone + H2O
adenine 9-beta-D-riboside + 3-methylbut-2-enal + ?
show the reaction diagram
-, O22213, Q9LTS3
-
-
-
?
N6-(2-isopentenyl)adenine 9-beta-D-riboside + 3',5'-dimethoxy-4-hydroxyacetophenone + H2O
adenine 9-beta-D-riboside + 3-methylbut-2-enal + ?
show the reaction diagram
-, O22213, Q9LTS3
-
-
-
?
N6-(2-isopentenyl)adenine 9-beta-D-riboside + ferricyanide + H2O
adenine 9-beta-D-riboside + 3-methylbut-2-enal + ferrocyanide
show the reaction diagram
-, O22213, Q9LTS3
-
-
-
?
N6-(2-isopentenyl)adenine 9-beta-D-riboside + ferricytochrome c + H2O
adenine 9-beta-D-riboside + 3-methylbut-2-enal + ferrocytochrome c
show the reaction diagram
-, O22213, Q9LTS3
-
-
-
?
N6-(2-isopentenyl)adenine 9-beta-D-riboside + NAD+ + H2O
adenine 9-beta-D-riboside + 3-methylbut-2-enal + ?
show the reaction diagram
-, O22213, Q9LTS3
-
-
-
?
N6-(2-isopentenyl)adenine 9-beta-D-riboside + O2 + H2O
adenine 9-beta-D-riboside + 3-methylbut-2-enal + ?
show the reaction diagram
-, O22213, Q9LTS3
-
-
-
?
N6-(2-isopentenyl)adenine 9-beta-D-riboside + oxidized 2,6-dichlorophenolindophenol + H2O
adenine 9-beta-D-riboside + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-, O22213, Q9LTS3
-
-
-
?
N6-(2-isopentenyl)adenine 9-beta-D-riboside 5'-phosphate + 2,3-dimethoxy-5-methyl-1,4-benzoquinone + H2O
adenine 9-beta-D-riboside 5'-phosphate + 3-methylbut-2-enal + ?
show the reaction diagram
-, O22213, Q9LTS3
-
-
-
?
N6-(2-isopentenyl)adenine 9-glucoside + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
low activity
-
-
?
N6-(2-isopentenyl)adenine 9-riboside + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
-
-
-
?
N6-(2-isopentenyl)adenine 9-riboside + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
-
-
-
?
N6-(2-isopentenyl)adenine 9-riboside-5'-monophosphate + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
low activity
-
-
?
N6-(2-isopentenyl)adenose + 2,6-dichlorophenolindophenol
adenosine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
N6-(2-isopentenyl)adenosine + 2,6-dichlorophenol indophenol
?
show the reaction diagram
-
very effective electron acceptor
-
-
?
N6-(2-isopentenyl)adenosine + FAD
adenosine + 3-methylbut-2-enal + FADH2
show the reaction diagram
Q9T0N8
-
-
-
?
N6-(4-hydroxy-2-butynyl)adenine + 2,6-dichlorophenol indophenol
?
show the reaction diagram
-
very effective electron acceptor
-
-
?
N6-(DELTA2-isopentenyl)adenine + 2,6-dichlorophenolindophenol + H2O
adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
Q9T0N8
artificial electron acceptor
-
-
?
N6-(DELTA2-isopentenyl)adenine + 4-methylcatechol + H2O
adenine + 3-methylbut-2-enal + reduced 4-methylcatechol
show the reaction diagram
Q9T0N8
-
-
-
?
N6-(DELTA2-isopentenyl)adenine + acceptor + H2O
adenine + 3-methylbut-2-enal + reduced acceptor
show the reaction diagram
-
-
-
-
?
N6-(DELTA2-isopentenyl)adenine + acetosyringone + H2O
adenine + 3-methylbut-2-enal + reduced acetosyringone
show the reaction diagram
Q9T0N8
-
-
-
?
N6-(DELTA2-isopentenyl)adenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
Q9T0N8
-
-
-
?
N6-(DELTA2-isopentenyl)adenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
-
-, oxidative degradation of cytokinins
-
-
ir
N6-(DELTA2-isopentenyl)adenine + guaiacol + H2O
adenine + 3-methylbut-2-enal + reduced guaiacol
show the reaction diagram
Q9T0N8
-
-
-
?
N6-(DELTA2-isopentenyl)adenosine + 2,6-dichlorophenolindophenol + H2O
adenosine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
Q9T0N8
artificial electron acceptor
-
-
?
N6-(DELTA2-isopentenyl)adenosine + 4-methylcatechol + H2O
adenosine + 3-methylbut-2-enal + reduced 4-methylcatechol
show the reaction diagram
Q9T0N8
-
-
-
?
N6-(DELTA2-isopentenyl)adenosine + acetosyringone + H2O
adenosine + 3-methylbut-2-enal + reduced acetosyringone
show the reaction diagram
Q9T0N8
-
-
-
?
N6-(DELTA2-isopentenyl)adenosine + FAD + H2O
adenosine + 3-methylbut-2-enal + FADH2
show the reaction diagram
Q9T0N8
-
-
-
?
N6-(DELTA2-isopentenyl)adenosine + guaiacol + H2O
adenosine + 3-methylbut-2-enal + reduced guaiacol
show the reaction diagram
Q9T0N8
-
-
-
?
N6-benzyladenine + electron acceptor
?
show the reaction diagram
-
-
-
-
?
N6-benzyladenine + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
-
-
-
-
ir
N6-benzyladenine + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
Q9T0N8
substrate binding mechanism and structure, overview
-
-
ir
N6-dimethylallyladenine + 2,6-dichlorophenolindophenol
adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
i.e. isopentenyladenine, reaction via an imine intermediate
-
-
?
N6-dimethylallyladenine + acceptor + H2O
adenine + 3-methylbut-2-enal + reduced acceptor
show the reaction diagram
-
-
-
-
?
N6-dimethylallyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
-
-
-
-
?
N6-dimethylallyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
-
-
-
-
ir
N6-dimethylallyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
Q9FE45
-
-
-
ir
N6-dimethylallyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
-
enzyme catalyzes the irreversible degradation of cytokinins
-
-
ir
N6-dimethylallyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
Q8H6F6
enzyme catalyzes the irreversible degradation of cytokinins
-
-
ir
N6-dimethylallyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
-, Q9FUJ3
enzyme catalyzes the irreversible degradation of cytokinins
-
-
ir
N6-dimethylallyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
Q9T0N8
enzyme catalyzes the irreversible degradation of cytokinins
-
-
ir
N6-dimethylallyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
-
enzyme catalyzes the irreversible degradation of cytokinins
-
-
ir
N6-dimethylallyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
-
i.e. isopentenyladenine
-
-
ir
N6-dimethylallyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
Q9FUJ3
i.e. isopentenyladenine
-
-
ir
N6-dimethylallyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
Q9T0N8
i.e. isopentenyladenine
-
-
ir
N6-dimethylallyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
-
i.e. isopentenyladenine
-
-
ir
N6-dimethylallyladenine + FAD + H2O2
adenine + 3-methylbut-2-enal + FADH2 + O2
show the reaction diagram
-
i.e. isopentenyladenine
-
-
?
N6-dimethylallyladenosine + FAD + H2O
adenosine + 3-methylbut-2-enal + FADH2
show the reaction diagram
Q9T0N8
i.e. isopentenyladenosine
-
-
ir
N6-hexyladenine + electron acceptor
hexanal + adenine + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
N6-isopentenyl-2-(2-hydroxyethylamino)-9-methyladenine + electron acceptor
3-methyl-2-butenal + 2-hydroxyethylamino-9-methyladenine + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
N6-isopentenyl-2-methylthioadenine + electron acceptor
3-methyl-2-butenal + 2-methylthioadenine + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
N6-isopentenyladenine + 2,6-dichlorophenolindophenol
adenine + 3-methyl-2-butenal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
N6-isopentenyladenine + CuCl2
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
A0MQ40
-
-
-
?
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
-
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
?
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
?
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
-
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
ir
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
ir
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
-
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
-
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
ir
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
ir
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
ir
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
-
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
?
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
?
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
ir
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
-
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
ir
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
-
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
-
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
ir
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
-
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
ir
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
A2TLJ3, -
-
-
-
ir
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
FAD is cofactor
-
ir
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
FAD is cofactor
-
ir
N6-isopentenyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
-
-
-
-
ir
N6-isopentenyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
Q9T0N8
oxidative degradation, substrate binding mechanism and structure, overview
-
-
ir
N6-isopentenyladenine + oxidized 2,6-dichlorophenolindophenol
adenine + 3-methylbut-2-enol + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
N6-isopentenyladenine + oxidized 2,6-dichlorophenolindophenol
adenine + 3-methylbut-2-enol + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
B6V8F7, Q9T0N8
-
-
-
?
N6-isopentenyladenine 9-beta-D-glucoside + oxidized 2,6-dichlorophenolindophenol
adenine 9-beta-D-glucoside + 3-methylbut-2-enol + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
B6V8F7, Q9T0N8
-
-
-
?
N6-isopentenyladenosine + 2,6-dichlorophenolindophenol
3-methylbut-2-enal + adenosine + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
-
N6-isopentenyladenosine + electron acceptor
3-methylbut-2-enal + adenosine + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
N6-isopentenyladenosine + electron acceptor
3-methylbut-2-enal + adenosine + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
N6-isopentenyladenosine + electron acceptor
3-methylbut-2-enal + adenosine + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
N6-isopentenyladenosine + oxidized 2,6-dichlorophenolindophenol
adenosine + 3-methylbut-2-enol + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
B6V8F7, Q9T0N8
-
-
-
?
N6-methyl-isopentenyladenine + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
very low activity
-
-
?
O-beta-D-glucosyl-trans-zeatin + oxidized 2,6-dichlorophenolindophenol
?
show the reaction diagram
B6V8F7, Q9T0N8
-
-
-
?
o-topolin + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
very low activity
-
-
?
p-topolin + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
very low activity
-
-
?
p-topolin + FAD
?
show the reaction diagram
-
-
-
-
?
thidiazuron + electron acceptor
?
show the reaction diagram
-
-
-
-
?
trans-zeatin + 2,3-dimethoxy-5-methyl-1,4-benzoquinone + H2O
?
show the reaction diagram
-, O22213, Q9LTS3
-
-
-
?
trans-zeatin + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
very effective substrate
-
-
?
trans-zeatin + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-, Q84U27, Q8H6F6, Q94KI4
cis-zeatin is utilized only by some tissue-specific forms but not by HvCKX2
-
-
?
trans-zeatin + acceptor + H2O
?
show the reaction diagram
-
-
-
-
?
trans-zeatin + acceptor + H2O
?
show the reaction diagram
-
preferred substrate of isozymes AtCKX4 and AtCKX1
-
-
?
trans-zeatin + acceptor + H2O
?
show the reaction diagram
-
preferred substrate of isozymes ZmCKX1 and ZmCKX10
-
-
?
trans-zeatin + electron acceptor
(2E)-4-hydroxy-3-methylbut-2-enal + adenine + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
trans-zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
-
-
-
-
-
trans-zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
-, Q9FUJ3
-
-
-
ir
trans-zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
-, Q9FUJ3
enzyme catalyzes the irreversible degradation of cytokinins
-
-
ir
trans-zeatin + FAD + H2O
? + FADH2
show the reaction diagram
-
-
-
-
ir
trans-zeatin + FAD + H2O
? + FADH2
show the reaction diagram
Q9T0N8
substrate binding mechanism and structure, overview
-
-
ir
trans-zeatin + oxidized 2,6-dichlorophenolindophenol
?
show the reaction diagram
-
-
-
-
?
trans-zeatin + oxidized 2,6-dichlorophenolindophenol
?
show the reaction diagram
B6V8F7, Q9T0N8
-
-
-
?
trans-zeatin 9-glucoside + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
low activity
-
-
?
trans-zeatin 9-riboside + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
very effective substrate
-
-
?
trans-zeatin O-glucoside + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-, Q84U27, Q8H6F6, Q94KI4
-
-
-
?
trans-zeatin riboside + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-, Q84U27, Q8H6F6, Q94KI4
-
-
-
?
trans-zeatin riboside O-glucoside + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-, Q84U27, Q8H6F6, Q94KI4
-
-
-
?
trans-zeatin ribotide + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-, Q84U27, Q8H6F6, Q94KI4
-
-
-
?
trans-zeatin-9-glucoside + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-, Q84U27, Q8H6F6, Q94KI4
-
-
-
?
zeatin + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
zeatin + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
zeatin + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
zeatin + 2,6-dichlorophenolindophenol
4-hydroxy-3-methylbut-2-enal + adenine + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
zeatin + electron acceptor
4-hydroxy-3-methylbut-2-enal + adenine + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
zeatin + electron acceptor
4-hydroxy-3-methylbut-2-enal + adenine + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
zeatin + electron acceptor
4-hydroxy-3-methylbut-2-enal + adenine + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
zeatin + electron acceptor
4-hydroxy-3-methylbut-2-enal + adenine + reduced electron acceptor
show the reaction diagram
A2TLJ3, -
-
-
-
ir
zeatin + FAD
? + FADH2
show the reaction diagram
Q9T0N8
-
-
-
?
zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
-
-
-
-
ir
zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
Q9FE45
-
-
-
ir
zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
-
-
-
-
ir
zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
-
enzyme catalyzes the irreversible degradation of cytokinins
-
-
ir
zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
Q8H6F6
enzyme catalyzes the irreversible degradation of cytokinins
-
-
ir
zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
-
enzyme catalyzes the irreversible degradation of cytokinins
-
-
ir
zeatin + FAD + H2O
adenine + 4-hydroxy-3-methylbut-2-enal + FADH2
show the reaction diagram
-
-
-
-
ir
zeatin riboside + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
Q9T0N8
-
-
-
-
zeatin riboside + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
m-topolin + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
very low activity
-
-
?
additional information
?
-
Q9FE45
analysis of enzyme activity regulation
-
-
-
additional information
?
-
-
cytokinins stimulate pigment formation and nitrogen fixation
-
-
-
additional information
?
-
-
effects of exogenously applied auxins on cytokinin levels in seedlings, overview, exogenously applied cytokinins increase the glycosylation level of endogenous cytokinins, overview
-
-
-
additional information
?
-
-
endogenous cytokinin levels after induction with N6-benzylaminopurine, overview, apoplastic enzyme degradation pathway
-
-
-
additional information
?
-
-
enzyme expression changes differently due to stress response in the 2 pea cultivars
-
-
-
additional information
?
-
Q9T0N8
enzyme expression is co-localized with laccase, enzyme activity probably is associated with plant phenolic oxidation
-
-
-
additional information
?
-
Q9T0N8
enzyme has a major role in control of cytokinin plant hormone levels
-
-
-
additional information
?
-
-
enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes
-
-
-
additional information
?
-
-
enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes during infection, cytokinins are involved in pathogenesis
-
-
-
additional information
?
-
-
enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes, influences chloroplast development
-
-
-
additional information
?
-
Q8H6F6
enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes, influences chloroplast development
-
-
-
additional information
?
-
-, Q9FUJ3
enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes, influences chloroplast development
-
-
-
additional information
?
-
Q9T0N8
enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes, influences chloroplast development
-
-
-
additional information
?
-
Q9T0N8
specificity for electron acceptors, overview
-
-
-
additional information
?
-
Q9T0N8
substrate specificity with cytokinins, overiew
-
-
-
additional information
?
-
-, Q9FUJ3
the free bases and their ribosides are the preferred substrates
-
-
-
additional information
?
-
Q9T0N8
the substrate binding site is located at amino acid residues 245-491
-
-
-
additional information
?
-
-, Q84U27, Q8H6F6, Q94KI4
tissue and isozyme-specific substrate specificity
-
-
-
additional information
?
-
-, Q84U27, Q8H6F6, Q94KI4
tissue and isozyme-specific substrate specificity, recombinant HvCKX2 prefers cytokinin ribosides as substrates
-
-
-
additional information
?
-
-
involvement of cytokinin oxidase/dehydrogenase in regulation of cytokinin levels in leaves
-
-
-
additional information
?
-
-
reduced expression of OsCKX2 causes cytokinin accumulation in inflorescence meristems and increases the number of reproductive organs, resulting in enhanced grain yield
-
-
-
additional information
?
-
-
irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde
-
-
-
additional information
?
-
-
irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde
-
-
-
additional information
?
-
Q9FUJ1
irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde
-
-
-
additional information
?
-
-
irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde
-
-
-
additional information
?
-
-
irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde
-
-
-
additional information
?
-
A2TLJ3, -
irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde
-
-
-
additional information
?
-
A0MQ40
irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde
-
-
-
additional information
?
-
-
irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde, by controlling the cytokinin level enzyme controls proliferation and differentiation of plant cells
-
-
-
additional information
?
-
-
irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde, important for the regulation of early development of the plant embryo
-
-
-
additional information
?
-
A0MQ40
irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde, results suggest that CKX is responsible for the changes in endogenous cytokinin pool in gibberellic acid-treated plants and most probably this enzyme represents an important link in gibberellic acid/cytokinin cross talk
-
-
-
additional information
?
-
-
AtCKX2 prefers p-quinones and 2,6-dichlorophenol indophenol as electron acceptors, low reactivity with oxygen, no activity with dihydrozeatin, no activity with vitamin K1 as electron acceptor
-
-
-
additional information
?
-
-
no activity with zeatin, zeatin riboside, or N6-(2-hydroxybenzyl)adenine
-
-
-
additional information
?
-
-
laccase and peroxidase catalyze oxidative cleavage of hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one (DIMBOA) to 4-nitrosoresorcinol-1-monomethyl ether (coniferron) which serves as a weak electron acceptor of CKX1. The oxidation of DIMBOA and coniferron generates transitional free radicals that are used by CKX1 as effective electron acceptors. hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-glucoside is not active as an electron acceptor for isozyme CKX1
-
-
-
additional information
?
-
-, O22213, Q9LTS3
acceptor and substrate specificity, overview. The enzyme catalyzes the irreversible cleavage of N6-side chains from cytokinins. CKXs also show low cytokinin oxidase activity, but molecular oxygen is a comparatively poor electron acceptor. Vacuolar AtCKX enzymes in certain conditions degrade N6-(2-isopentenyl)adenine di- and triphosphates two to 5times more effectively than its monophosphate
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cis-zeatin + acceptor + H2O
?
show the reaction diagram
-
-
-
-
?
N6-(2-isopentenyl)adenine + FAD
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
Q9T0N8
the natural terminal electron acceptor probably is a p-quinone or a similar compound, high substrate specificity for N6-(2-isopentenyl)adenine
-
-
?
N6-(2-isopentenyl)adenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
-
-
-
-
ir
N6-(DELTA2-isopentenyl)adenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
Q9T0N8
-
-
-
?
N6-(DELTA2-isopentenyl)adenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
-
oxidative degradation of cytokinins
-
-
ir
N6-(DELTA2-isopentenyl)adenosine + FAD + H2O
adenosine + 3-methylbut-2-enal + FADH2
show the reaction diagram
Q9T0N8
-
-
-
?
N6-dimethylallyladenine + acceptor + H2O
adenine + 3-methylbut-2-enal + reduced acceptor
show the reaction diagram
-
-
-
-
?
N6-dimethylallyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
-
-
-
-
?
N6-dimethylallyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
-
-
-
-
ir
N6-dimethylallyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
Q9FE45
-
-
-
ir
N6-dimethylallyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
-
enzyme catalyzes the irreversible degradation of cytokinins
-
-
ir
N6-dimethylallyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
Q8H6F6
enzyme catalyzes the irreversible degradation of cytokinins
-
-
ir
N6-dimethylallyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
-, Q9FUJ3
enzyme catalyzes the irreversible degradation of cytokinins
-
-
ir
N6-dimethylallyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
Q9T0N8
enzyme catalyzes the irreversible degradation of cytokinins
-
-
ir
N6-dimethylallyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
-
enzyme catalyzes the irreversible degradation of cytokinins
-
-
ir
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
A2TLJ3, -
-
-
-
ir
N6-isopentenyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
Q9T0N8
oxidative degradation
-
-
ir
trans-zeatin + acceptor + H2O
?
show the reaction diagram
-
-
-
-
?
trans-zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
-, Q9FUJ3
enzyme catalyzes the irreversible degradation of cytokinins
-
-
ir
zeatin + electron acceptor
4-hydroxy-3-methylbut-2-enal + adenine + reduced electron acceptor
show the reaction diagram
A2TLJ3, -
-
-
-
ir
zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
-
-
-
-
ir
zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
Q9FE45
-
-
-
ir
zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
-
enzyme catalyzes the irreversible degradation of cytokinins
-
-
ir
zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
Q8H6F6
enzyme catalyzes the irreversible degradation of cytokinins
-
-
ir
zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
-
enzyme catalyzes the irreversible degradation of cytokinins
-
-
ir
zeatin + FAD + H2O
adenine + 4-hydroxy-3-methylbut-2-enal + FADH2
show the reaction diagram
-
-
-
-
ir
cis/trans-zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
Q9T0N8
enzyme catalyzes the irreversible degradation of cytokinins
-
-
ir
additional information
?
-
Q9FE45
analysis of enzyme activity regulation
-
-
-
additional information
?
-
-
cytokinins stimulate pigment formation and nitrogen fixation
-
-
-
additional information
?
-
-
effects of exogenously applied auxins on cytokinin levels in seedlings, overview, exogenously applied cytokinins increase the glycosylation level of endogenous cytokinins, overview
-
-
-
additional information
?
-
-
endogenous cytokinin levels after induction with N6-benzylaminopurine, overview, apoplastic enzyme degradation pathway
-
-
-
additional information
?
-
-
enzyme expression changes differently due to stress response in the 2 pea cultivars
-
-
-
additional information
?
-
Q9T0N8
enzyme expression is co-localized with laccase, enzyme activity probably is associated with plant phenolic oxidation
-
-
-
additional information
?
-
Q9T0N8
enzyme has a major role in control of cytokinin plant hormone levels
-
-
-
additional information
?
-
-
enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes
-
-
-
additional information
?
-
-
enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes during infection, cytokinins are involved in pathogenesis
-
-
-
additional information
?
-
-
enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes, influences chloroplast development
-
-
-
additional information
?
-
Q8H6F6
enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes, influences chloroplast development
-
-
-
additional information
?
-
-, Q9FUJ3
enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes, influences chloroplast development
-
-
-
additional information
?
-
Q9T0N8
enzyme has a regulatory role in cytokinin metabolism and cytokinin-dependent processes, influences chloroplast development
-
-
-
additional information
?
-
-
involvement of cytokinin oxidase/dehydrogenase in regulation of cytokinin levels in leaves
-
-
-
additional information
?
-
-
reduced expression of OsCKX2 causes cytokinin accumulation in inflorescence meristems and increases the number of reproductive organs, resulting in enhanced grain yield
-
-
-
additional information
?
-
-
irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde
-
-
-
additional information
?
-
-
irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde
-
-
-
additional information
?
-
Q9FUJ1
irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde
-
-
-
additional information
?
-
-
irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde
-
-
-
additional information
?
-
-
irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde
-
-
-
additional information
?
-
A2TLJ3, -
irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde
-
-
-
additional information
?
-
A0MQ40
irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde
-
-
-
additional information
?
-
-
irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde, by controlling the cytokinin level enzyme controls proliferation and differentiation of plant cells
-
-
-
additional information
?
-
-
irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde, important for the regulation of early development of the plant embryo
-
-
-
additional information
?
-
A0MQ40
irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde, results suggest that CKX is responsible for the changes in endogenous cytokinin pool in gibberellic acid-treated plants and most probably this enzyme represents an important link in gibberellic acid/cytokinin cross talk
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2,6-dichlorophenolindophenol
-
in vitro 4000fold faster reaction than with the native acceptor
FAD
-
covalently bound
FAD
Q9T0N8
covalently bound, redox potential analysis
FAD
-, Q84U27, Q8H6F6, Q94KI4
binding motif
FAD
Q94KI5
binding motif
FAD
-
binding site structure comprising amino acid residues 40-244 and 492-534
FAD
Q9T0N8
binding site comprising amino acid residues 69-105, covalent binding through a histidine residue
FAD
D3JAJ5
flavoenzyme
flavin
B6V8F7, Q9T0N8
;
flavin
-, O22213, Q9LTS3
flavoprotein; flavoprotein; flavoprotein
additional information
Q9T0N8
enzyme shows a preference for quinones as electron acceptors
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Cu2+
-
dependent
Cu2+
-
dependent
Cu2+
-
dependent
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
(2,6-dichloro-pyridin-4-yl)-carbamic acid benzyl ester
-
-
-
(2-chloro-pyridin-4-yl)-carbamic acid 4-chloro-phenyl ester
-
-
-
2,4-Dichlorophenoxyacetic acid
-
inhibits the enzyme by up to 31%
2-amino-6-(3-methoxyanilino)purine
-
-
2-chloro-6-(2-hydroxyanilino)purine
-
-
2-chloro-6-(2-methoxyanilino)purine
-
-
2-chloro-6-(3-chloroanilino)purine
-
-
2-chloro-6-(3-fluoroanilino)purine
-
-
2-chloro-6-(3-hydroxyanilino)purine
-
-
2-chloro-6-(3-methoxyanilino)-9-isopropylpurine
-
-
2-chloro-6-(3-methoxyanilino)-9-methylpurine
-
-
2-chloro-6-(3-methoxyanilino)purine
-
-
2-chloro-6-(4-chloroanilino)purine
-
-
2-chloro-6-(4-fluoroanilino)purine
-
-
2-chloro-6-(4-hydroxyanilino)purine
-
-
2-chloro-6-(4-methoxyanilino)purine
-
-
2-chloro-6-anilinopurine
-
-
2-fluoro-6-(3-methoxyanilino)purine
-
-
2-methylthio-6-(3-methoxyanilino)purine
-
-
2-nitro-6-(3-methoxyanilino)purine
-
-
24-epibrassinolide
-
inhibits both cytokinin oxidase activity and expression. Treatment with 0.4 mM 24-epibrassinolide leads to elevated cytokinin levels in roots and shoots, that are maintained only in the presence of 24-epibrassinolide, while the hormone removal leads to return of cytokinin concentration to the control level initially in the roots and then in the shoots
6-(3-methoxyanilino)purine
-
-
adenine
-
slight product inhibition
alpha-naphthaleneacetic acid
-
inhibits the enzyme by up to 54%
diphenylene iodonium
-
-
N-(1,2,3-thidiazol-5-yl)-N'-phenylurea
-
-
-
N-(2,6-4-dichloro-pyridin-4-yl)-N'-benzyl-N'-methylurea
-
-
-
N-(2,6-dichloro-pyridin-4-yl)-N'-benzylurea
-
-
-
N-(2,6-dichloro-pyridin-4-yl)-N'-phenoxyurea
-
-
-
N-(2,6-dichloro-pyridin-4-yl)-N'-phenylurea
-
-
-
N-(2-amino-6-chloro-pyridin-4-yl)-N'-benzylurea
-
-
-
N-(2-amino-6-chloro-pyridin-4-yl)-N'-phenylurea
-
one of the most potent inhibitors of CKO
-
N-(2-amino-pyridin-4-yl)-N'-phenylurea
-
one of the most potent inhibitors of CKO
-
N-(2-chloro-1-oxy-pyridin-4-yl)-N'-phenylurea
-
-
-
N-(2-chloro-4-pyridyl)-N'-phenylurea
-
-
N-(2-chloro-4-pyridyl-N'-5-fluoro-phenylurea)
-
-
N-(2-chloro-6-methoxy-pyridin-4-yl)-N'-benzylurea
-
-
-
N-(2-chloro-pyridin-4-yl)-N'-benzylurea
-
-
-
N-(2-chloro-pyridin-4-yl)-N'-phenylurea
-
the compound binds in a planar conformation and competes for the same binding site with natural substrates
-
N-imidazo[1,2-a]pyridin-4-yl-N'-phenylurea
-
-
-
N6-(2-bromo-2-isopentenyl)adenine
-
weak inhibition
N6-(2-butynyl)adenine
-
irreversible inhibition
N6-(3-hydroxy-2-phenylethyl)-2-(3-hydroxypropylamino)-9-isopentenyladenine
-
-
N6-(4-hydroxy-2-butynyl)adenine
-
irreversible inhibition
N6-(buta-2,3-dienyl)adenine
-
strong irreversible mechanism based inhibition
N6-(penta-2,3-dienyl)adenine
-
strong irreversible mechanism based inhibition
N6-benzyl-2-(2-hydroxyethylamino)-9-mehtyladenine
-
-
N6-benzyl-2-amino-9-isoproyladenine
-
-
N6-benzyladenine
-
strongly stimulates enzyme activity by up to 180%
N6-but-2,3-dienyl-aminopurine
-
mechanism-based irreversible (suicide) inhibitor. IC50: 0.02 mM with N6-(2-isopentenyl)adenine as substrate, 0.0004 mM with N6-(2-isopentenyl)adenosine as substrate and less than 0.0001 mM with zeatin as substrate
N6-isopentenyl-N-methylaminopurine
Q9T0N8
; structural analogue of isopentenyladenine
N6-pent-2,3-dienyl aminopurine
-
10%, 52%, and 86% inhibition at concentrations of 0.001 mM, 0.01 mM and 0.1 mM
phenylhydrazine
-
-
thidiazuron
-
-
trans-zeatin
-
enhances enzyme activity by 35%
diphenylurea
Q9T0N8
; competitive
additional information
Q9T0N8
enzyme is strongly inhibited by flavin-protein oxidoreductase inhibitors
-
additional information
-
UV-B irradiation reduces enzyme activity
-
additional information
A0MQ40
cultivation in the presence of gibberillic acid reduces enzyme activity in leaves of cv. Manuela
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one
-
-
-
imidazole
-
in complex with Cu2+ enhances activity
additional information
Q94KI5
isozymes TaCKX5a/b, TaCKX6a, and TaCKX2a expression are induced by pathogen infection, i.e. by Fusarium sp. or Puccinia sp.
-
additional information
Q9FE45
enzyme is induced by cytokinins
-
additional information
-
cytokinins induce enzyme activity, predominantly of the glycosylated isozyme
-
additional information
-
cytokinin-induced up-regulation of enzyme activity, e.g. by N6-benzylaminopurine application on the plant leaf or callus surface leads to about 2.5fold increased activity after 4 h, overview
-
additional information
-
elevated temperature around 35C increases enzyme activity
-
additional information
A0MQ40
cultivation in the presence of gibberillic acid increases enzyme activity in leaves of cv. Scinado
-
additional information
-
auxin treatment induces AtCKX6 gene and increases enzyme level
-
additional information
-
dark-treatment results in high increase in CKX activity, addition of kinetin in dark-treated leaves results in high increase of CKX activity compared with dark-treated leaves without kinetin
-
additional information
A2TLJ3, -
expression of the DhCKX gene is induced in roots, shoot apices and leaves, but not in stems, after treatment with 0.02 mM 6-benzylamino-purine
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.067
-
2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical
-
pH 5.0, temperature not specified in the publication
-
0.0041
-
4-nitrosoresorcinol-1-monomethyl ether
-
pH 5.0, in the presence of peroxidase, temperature not specified in the publication
-
0.0062
-
4-nitrosoresorcinol-1-monomethyl ether
-
pH 5.0, in the presence of laccase, temperature not specified in the publication
-
0.0113
-
4-nitrosoresorcinol-1-monomethyl ether
-
pH 7.0, in the presence of peroxidase, temperature not specified in the publication
-
0.192
-
4-nitrosoresorcinol-1-monomethyl ether
-
pH 5.0, temperature not specified in the publication
-
0.046
-
cis-zeatin
Q9T0N8
pH 7.0
0.0089
-
hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one
-
pH 5.0, in the presence of peroxidase, temperature not specified in the publication
-
0.0109
-
hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one
-
pH 5.0, in the presence of laccase, temperature not specified in the publication
-
0.0345
-
hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one
-
pH 7.0, in the presence of laccase, temperature not specified in the publication
-
0.113
-
hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one
-
pH 7.0, in the presence of peroxidase, temperature not specified in the publication
-
0.092
-
N6-(2-butynyl)adenine
-
37C
0.0011
-
N6-(2-isopentenyl)adenine
-
37C
0.0011
-
N6-(2-isopentenyl)adenine
-
wild type enzyme, pH and temperature not specified in the publication
0.0015
-
N6-(2-isopentenyl)adenine
-
with 2,3-dimethoxy-5-methyl-p-benzoquinone, pH 7.0, 37C
0.003
-
N6-(2-isopentenyl)adenine
-
mutant enzyme E381A, pH and temperature not specified in the publication
0.0032
-
N6-(2-isopentenyl)adenine
-
mutant enzyme E381S, pH and temperature not specified in the publication
0.38
-
N6-(4-hydroxy-2-butynyl)adenine
-
37C
0.001
-
N6-(DELTA2-isopentenyl)adenine
-
transgenic plant cells conditionally overexpressing isopentenyltransferase, cell culture medium induced by tetracycline, pH 6.0, 37C
0.0015
-
N6-(DELTA2-isopentenyl)adenine
-
transgenic plant cells conditionally overexpressing isopentenyltransferase, cell extract induced by tetracycline, pH 6.0, 37C
0.0017
-
N6-(DELTA2-isopentenyl)adenine
-
transgenic plant cells conditionally overexpressing isopentenyltransferase, cell culture medium induced by N6-benzylaminopurine, pH 6.0, 37C
0.0022
-
N6-(DELTA2-isopentenyl)adenine
-
transgenic plant cells conditionally overexpressing isopentenyltransferase, cell extract, pH 6.0, 37C
0.0023
-
N6-(DELTA2-isopentenyl)adenine
-
transgenic plant cells conditionally overexpressing isopentenyltransferase, cell extract induced by N6-benzylaminopurine, pH 6.0, 37C
0.0025
-
N6-(DELTA2-isopentenyl)adenine
-
transgenic plant cells conditionally overexpressing isopentenyltransferase, cell culture medium, pH 6.0, 37C
0.0015
-
N6-dimethylallyladenine
Q9T0N8
pH 7.0
0.015
-
N6-dimethylallyladenine
-
pH 9.0
0.04
-
N6-dimethylallyladenine
-
pH 6.5
0.054
-
N6-dimethylallyladenine
Q9T0N8
pH 7.0
0.0011
-
N6-isopentenyladenine
-
30C, pH 7.4
0.003
-
N6-isopentenyladenine
-
-
0.012
-
N6-isopentenyladenosine
-
30C, pH 7.4
0.015
-
N6-isopentenyladenosine
-
-
0.006
-
trans-zeatin
-
pH 9.0
0.01
-
trans-zeatin
-
-
0.014
-
trans-zeatin
Q9T0N8
pH 7.0
0.06
-
trans-zeatin
-
pH 6.5
0.011
-
trans-zeatin riboside
Q9T0N8
pH 7.0
0.009
-
zeatin
-
30C, pH 7.4
0.063
-
zeatin riboside
-
30C, pH 7.4
0.25
-
hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one
-
Km above 0.25 mM, pH 7.0, temperature not specified in the publication
-
additional information
-
additional information
Q9T0N8
kinetics, redox potential of electron acceptors
-
additional information
-
additional information
-
enzyme activity dynamics in relation to glycosylation level
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
169.6
-
1,4-Naphthoquinone
Q9T0N8
pH 6.5, recombinant enzyme
143.6
-
2,3-dimethoxy-5-methyl-1,4-benzoquinone
Q9T0N8
pH 6.5, recombinant enzyme
178.4
-
2,6-dichlorophenolindophenol
Q9T0N8
pH 6.5, recombinant enzyme
278
-
2,6-dichlorophenolindophenol
Q9T0N8
pH 8.0, recombinant enzyme
0.3
-
caffeic acid
Q9T0N8
pH 6.5, recombinant enzyme
123.1
-
coenzyme Q1
Q9T0N8
pH 6.5, recombinant enzyme
0.8
-
cytochrome c
Q9T0N8
pH 6.5, recombinant enzyme
1
-
daphnoretin
Q9T0N8
pH 6.5, recombinant enzyme
0.13
-
N-methyl-isopentenyladenine
-
pH 6.5, 25C
5.4
-
N6-(2-isopentenyl)adenine
-
with 2,3-dimethoxy-5-methyl-p-benzoquinone, pH 7.0, 37C
40
-
N6-(2-isopentenyl)adenine
-
pH 6.5, 25C
143.6
-
N6-(2-isopentenyl)adenine
Q9T0N8
pH 6.5, recombinant enzyme, with cofactor 2,3-dimethoxy-5-methyl-1,4-benzoquinone
129.8
-
N6-(2-isopentenyl)adenosine
Q9T0N8
pH 6.5, recombinant enzyme, with cofactor 2,3-dimethoxy-5-methyl-1,4-benzoquinone
128
-
N6-isopentenyladenine
-
30C, pH 7.4
62
-
N6-isopentenyladenosine
-
30C, pH 7.4
0.3
-
o-coumaric acid
Q9T0N8
pH 6.5, recombinant enzyme
0.1
-
O2
Q9T0N8
below, pH 8.0, recombinant enzyme
0.6
-
O2
Q9T0N8
pH 6.5, recombinant enzyme
0.23
-
p-topolin
-
pH 6.5, 25C
0.4
-
rosmarinic acid
Q9T0N8
pH 6.5, recombinant enzyme
1.4
-
scopoletin
Q9T0N8
pH 6.5, recombinant enzyme
0.9
-
spinach plantacyanine
Q9T0N8
pH 6.5, recombinant enzyme
-
2.3
-
vitamin K1
Q9T0N8
pH 6.5, recombinant enzyme
85.4
-
vitamin K3
Q9T0N8
pH 6.5, recombinant enzyme
135
-
zeatin
-
30C, pH 7.4
21
-
zeatin riboside
-
30C, pH 7.4
1.9
-
kinetin
-
pH 6.5, 25C
additional information
-
additional information
Q9T0N8
at pH above pH 6.5 polymerization of the quinone acceptors occur
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.01
-
N6-(buta-2,3-dienyl)adenine
-
-
0.026
-
N6-(penta-2,3-dienyl)adenine
-
-
0.012
-
N6-isopentenyl-N-methylaminopurine
Q9T0N8
recombinant enyme
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.004
-
(2,6-dichloro-pyridin-4-yl)-carbamic acid benzyl ester
-
mutant enzyme E381A, pH and temperature not specified in the publication
-
0.007
-
(2,6-dichloro-pyridin-4-yl)-carbamic acid benzyl ester
-
mutant enzyme E381S, pH and temperature not specified in the publication; mutant enzyme L492A, pH and temperature not specified in the publication
-
0.009
-
(2,6-dichloro-pyridin-4-yl)-carbamic acid benzyl ester
-
wild type enzyme, pH and temperature not specified in the publication
-
0.0002
-
(2-chloro-pyridin-4-yl)-carbamic acid 4-chloro-phenyl ester
-
mutant enzyme L492A, pH and temperature not specified in the publication
-
0.006
-
(2-chloro-pyridin-4-yl)-carbamic acid 4-chloro-phenyl ester
-
mutant enzyme E381A, pH and temperature not specified in the publication; mutant enzyme E381S, pH and temperature not specified in the publication
-
0.039
-
(2-chloro-pyridin-4-yl)-carbamic acid 4-chloro-phenyl ester
-
wild type enzyme, pH and temperature not specified in the publication
-
0.027
-
2-amino-6-(3-methoxyanilino)purine
-
-
0.0039
-
2-chloro-6-(3-chloroanilino)purine
-
-
0.0075
-
2-chloro-6-(3-fluoroanilino)purine
-
-
0.00375
-
2-chloro-6-(3-hydroxyanilino)purine
-
-
0.061
-
2-chloro-6-(3-methoxyanilino)-9-methylpurine
-
-
0.0019
-
2-chloro-6-(3-methoxyanilino)purine
-
-
0.0254
-
2-chloro-6-(4-chloroanilino)purine
-
-
0.05
-
2-chloro-6-(4-fluoroanilino)purine
-
-
0.08
-
2-chloro-6-anilinopurine
-
-
0.001
-
2-fluoro-6-(3-methoxyanilino)purine
-
-
0.014
-
2-nitro-6-(3-methoxyanilino)purine
-
-
0.005
-
N-(1,2,3-thidiazol-5-yl)-N'-phenylurea
-
mutant enzyme L492A, pH and temperature not specified in the publication
-
0.015
-
N-(1,2,3-thidiazol-5-yl)-N'-phenylurea
-
mutant enzyme E381A, pH and temperature not specified in the publication
-
0.017
-
N-(1,2,3-thidiazol-5-yl)-N'-phenylurea
-
mutant enzyme E381S, pH and temperature not specified in the publication
-
0.05
-
N-(1,2,3-thidiazol-5-yl)-N'-phenylurea
-
wild type enzyme, pH and temperature not specified in the publication
-
0.0011
-
N-(2,6-4-dichloro-pyridin-4-yl)-N'-benzyl-N'-methylurea
-
mutant enzyme E381A, pH and temperature not specified in the publication
-
0.0015
-
N-(2,6-4-dichloro-pyridin-4-yl)-N'-benzyl-N'-methylurea
-
mutant enzyme L492A, pH and temperature not specified in the publication
-
0.002
-
N-(2,6-4-dichloro-pyridin-4-yl)-N'-benzyl-N'-methylurea
-
mutant enzyme E381S, pH and temperature not specified in the publication; wild type enzyme, pH and temperature not specified in the publication
-
0.002
-
N-(2,6-dichloro-pyridin-4-yl)-N'-benzylurea
-
mutant enzyme L492A, pH and temperature not specified in the publication
-
0.003
-
N-(2,6-dichloro-pyridin-4-yl)-N'-benzylurea
-
mutant enzyme E381A, pH and temperature not specified in the publication
-
0.004
-
N-(2,6-dichloro-pyridin-4-yl)-N'-benzylurea
-
mutant enzyme E381S, pH and temperature not specified in the publication
-
0.009
-
N-(2,6-dichloro-pyridin-4-yl)-N'-benzylurea
-
wild type enzyme, pH and temperature not specified in the publication
-
0.005
-
N-(2,6-dichloro-pyridin-4-yl)-N'-phenoxyurea
-
mutant enzyme E381A, pH and temperature not specified in the publication; mutant enzyme L492A, pH and temperature not specified in the publication
-
0.008
-
N-(2,6-dichloro-pyridin-4-yl)-N'-phenoxyurea
-
mutant enzyme E381S, pH and temperature not specified in the publication
-
0.02
-
N-(2,6-dichloro-pyridin-4-yl)-N'-phenoxyurea
-
wild type enzyme, pH and temperature not specified in the publication
-
8e-05
-
N-(2,6-dichloro-pyridin-4-yl)-N'-phenylurea
-
mutant enzyme L492A, pH and temperature not specified in the publication
-
0.0004
-
N-(2,6-dichloro-pyridin-4-yl)-N'-phenylurea
-
mutant enzyme E381A, pH and temperature not specified in the publication
-
0.0005
-
N-(2,6-dichloro-pyridin-4-yl)-N'-phenylurea
-
mutant enzyme E381S, pH and temperature not specified in the publication
-
0.0045
-
N-(2,6-dichloro-pyridin-4-yl)-N'-phenylurea
-
wild type enzyme, pH and temperature not specified in the publication
-
0.006
-
N-(2-amino-6-chloro-pyridin-4-yl)-N'-benzylurea
-
mutant enzyme L492A, pH and temperature not specified in the publication
-
0.022
-
N-(2-amino-6-chloro-pyridin-4-yl)-N'-benzylurea
-
wild type enzyme, pH and temperature not specified in the publication
-
0.024
-
N-(2-amino-6-chloro-pyridin-4-yl)-N'-benzylurea
-
mutant enzyme E381A, pH and temperature not specified in the publication
-
0.032
-
N-(2-amino-6-chloro-pyridin-4-yl)-N'-benzylurea
-
mutant enzyme E381S, pH and temperature not specified in the publication
-
4e-05
-
N-(2-amino-6-chloro-pyridin-4-yl)-N'-phenylurea
-
mutant enzyme L492A, pH and temperature not specified in the publication
-
0.0006
-
N-(2-amino-6-chloro-pyridin-4-yl)-N'-phenylurea
-
mutant enzyme E381A, pH and temperature not specified in the publication
-
0.0007
-
N-(2-amino-6-chloro-pyridin-4-yl)-N'-phenylurea
-
mutant enzyme E381S, pH and temperature not specified in the publication
-
0.0018
-
N-(2-amino-6-chloro-pyridin-4-yl)-N'-phenylurea
-
wild type enzyme, pH and temperature not specified in the publication
-
4e-05
-
N-(2-amino-pyridin-4-yl)-N'-phenylurea
-
mutant enzyme L492A, pH and temperature not specified in the publication
-
0.002
-
N-(2-amino-pyridin-4-yl)-N'-phenylurea
-
wild type enzyme, pH and temperature not specified in the publication
-
0.032
-
N-(2-amino-pyridin-4-yl)-N'-phenylurea
-
mutant enzyme E381A, pH and temperature not specified in the publication
-
0.035
-
N-(2-amino-pyridin-4-yl)-N'-phenylurea
-
mutant enzyme E381S, pH and temperature not specified in the publication
-
0.005
-
N-(2-chloro-1-oxy-pyridin-4-yl)-N'-phenylurea
-
mutant enzyme L492A, pH and temperature not specified in the publication
-
0.015
-
N-(2-chloro-1-oxy-pyridin-4-yl)-N'-phenylurea
-
mutant enzyme E381A, pH and temperature not specified in the publication
-
0.017
-
N-(2-chloro-1-oxy-pyridin-4-yl)-N'-phenylurea
-
mutant enzyme E381S, pH and temperature not specified in the publication
-
0.13
-
N-(2-chloro-1-oxy-pyridin-4-yl)-N'-phenylurea
-
wild type enzyme, pH and temperature not specified in the publication
-
0.006
-
N-(2-chloro-6-methoxy-pyridin-4-yl)-N'-benzylurea
-
mutant enzyme L492A, pH and temperature not specified in the publication
-
0.015
-
N-(2-chloro-6-methoxy-pyridin-4-yl)-N'-benzylurea
-
mutant enzyme E381A, pH and temperature not specified in the publication
-
0.02
-
N-(2-chloro-6-methoxy-pyridin-4-yl)-N'-benzylurea
-
mutant enzyme E381S, pH and temperature not specified in the publication
-
0.022
-
N-(2-chloro-6-methoxy-pyridin-4-yl)-N'-benzylurea
-
wild type enzyme, pH and temperature not specified in the publication
-
0.0015
-
N-(2-chloro-pyridin-4-yl)-N'-benzylurea
-
mutant enzyme L492A, pH and temperature not specified in the publication
-
0.003
-
N-(2-chloro-pyridin-4-yl)-N'-benzylurea
-
mutant enzyme E381A, pH and temperature not specified in the publication
-
0.004
-
N-(2-chloro-pyridin-4-yl)-N'-benzylurea
-
mutant enzyme E381S, pH and temperature not specified in the publication
-
0.018
-
N-(2-chloro-pyridin-4-yl)-N'-benzylurea
-
wild type enzyme, pH and temperature not specified in the publication
-
0.035
-
N-(2-chloro-pyridin-4-yl)-N'-phenylurea
-
mutant enzyme L492A, pH and temperature not specified in the publication
-
0.042
-
N-(2-chloro-pyridin-4-yl)-N'-phenylurea
-
wild type enzyme, pH and temperature not specified in the publication
-
0.045
-
N-(2-chloro-pyridin-4-yl)-N'-phenylurea
-
mutant enzyme E381A, pH and temperature not specified in the publication
-
0.055
-
N-(2-chloro-pyridin-4-yl)-N'-phenylurea
-
mutant enzyme E381S, pH and temperature not specified in the publication
-
0.0001
-
N-imidazo[1,2-a]pyridin-4-yl-N'-phenylurea
-
mutant enzyme L492A, pH and temperature not specified in the publication
-
0.0004
-
N-imidazo[1,2-a]pyridin-4-yl-N'-phenylurea
-
mutant enzyme E381A, pH and temperature not specified in the publication
-
0.001
-
N-imidazo[1,2-a]pyridin-4-yl-N'-phenylurea
-
mutant enzyme E381S, pH and temperature not specified in the publication
-
0.006
-
N-imidazo[1,2-a]pyridin-4-yl-N'-phenylurea
-
wild type enzyme, pH and temperature not specified in the publication
-
0.02
2
N6-but-2,3-dienyl-aminopurine
-
mechanism-based irreversible (suicide) inhibitor. IC50: 0.02 mM with N6-(2-isopentenyl)adenine as substrate, 0.0004 mM with N6-(2-isopentenyl)adenosine as substrate and less than 0.0001 mM with zeatin as substrate
0.029
-
thidiazuron
-
-
0.0124
-
6-(3-methoxyanilino)purine
-
-
additional information
-
additional information
-
IC50-values above 0.1 mM for 2-chloro-6-(2-hydroxyanilino)purine, 2-chloro-6-(4-hydroxyanilino)purine, 2-chloro-6-(2-methoxyanilino)purine, 2-chloro-6-(4-methoxyanilino)purine, 2-methylthio-6-(3-methoxyanilino)purine and 2-chloro-6-(3-methoxyanilino)-9-isopropylpurine, no inhibition observed for 6-anilinopurine and trans-zeatin
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.0003
-
-
transgenic plant conditionally overexpressing isopentenyltransferase, cell culture medium, not induced; wild-type plant, cell extract and cell culture medium, not induced or tetracycline-treated cells
0.0004
-
-
transgenic plant conditionally overexpressing isopentenyltransferase, cell extract, not induced cells
0.002
-
-
wild-type plant and transgenic plant conditionally overexpressing isopentenyltransferase, cell extract, N6-benzylaminopurine-induced
0.004
-
-
transgenic plant conditionally overexpressing isopentenyltransferase, cell culture medium of N6-benzylaminopurine-induced cells
0.005
-
-
wild-type plant, cell culture medium of N6-benzylaminopurine-induced cells
0.0052
-
-
N6-(2-isopentenyl)adenine + 2,3-dimethoxy-5-methyl-p-benzoquinone, pH 7.0, 37C
0.0065
-
-
transgenic plant conditionally overexpressing isopentenyltransferase, cell culture medium of tetracycline-treated cells
30.6
-
-
purified recombinant enzyme
62.4
-
-
-
additional information
-
-
-
additional information
-
-
-
additional information
-
-
-
additional information
-
-, Q84U27, Q8H6F6, Q94KI4
-
additional information
-
Q9T0N8
activity in diverse tissues at pH 6.0 and pH 8.0, overview
additional information
-
-
assay development and optimization with 2,6-dichlorophenolindophenol as electron acceptor
additional information
-
-
specific activity in roots and leaves at different UV-B irradiation and temperatures levels, overview
additional information
-
-
specific activities given for all possible reactant pairs
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.5
-
-, Q84U27, Q8H6F6, Q94KI4
native overall enzyme activity in grains and leaves shows 2 maxima at pH 4.5 and at pH 7.0-7.5; recombinant HvCKX2 expressed in tobacco, native overall enzyme activity in grains and leaves shows 2 maxima at pH 4.5 and at pH 7.0-7.5
5
7.5
-, O22213, Q9LTS3
assay at; assay at; assay at
6
-
-
secreted enzyme in cell culture medium
6.8
-
-
-
7
7.5
-, Q84U27, Q8H6F6, Q94KI4
native overall enzyme activity in grains and leaves shows 2 maxima at pH 4.5 and at pH 7.0-7.5
7
7.5
-
with 2,3-dimethoxy-5-methyl-p-benzoquinone
7
-
Q9T0N8
assay at
7
-
-
assay at
7
-
-
reaction withz zeatin derivatives
7.1
-
-
assay at
7.2
-
Q9T0N8
assay at
7.2
-
-
reaction with N6-(2-isopentenyl)adenine derivatives
8
-
-
with 2,6-dichlorophenol indophenol
8.5
-
-
enzyme in cells
8.5
-
-
assay at
8.5
-
-
-
additional information
-
Q9T0N8
substrate-dependent pH-profile, overview
additional information
-
-
-
additional information
-
-
the 2 isozymes show largely different pH-optima
additional information
-
Phaseolus sp.
-
the 2 isozymes show largely different different pH-optima
additional information
-
Q9T0N8
activity in diverse tissues at pH 6.0 and pH 8.0, overview
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
10
-
pH 6.0: about 45% of maximal activity, pH 10.0: aout 55% of maximal activity
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
22
-
-
assay at room temperature
37
-
-, Q84U27, Q8H6F6, Q94KI4
assay at
37
-
-, O22213, Q9LTS3
assay at; assay at; assay at
37
-
-
assay at
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.9
-
-
calculated from sequence
5.93
-
D3JAJ5
sequence calculation
6
-
-, Q84U27, Q8H6F6, Q94KI4
sequence calculation
6.25
-
-
chromatofocusing
6.6
-
A2TLJ3, -
calculated from the deduced amino acid sequence
7.1
-
-, Q84U27, Q8H6F6, Q94KI4
sequence calculation
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Q9T0N8
of kernels, most abundant in
Manually annotated by BRENDA team
-, Q84U27, Q8H6F6, Q94KI4
-
Manually annotated by BRENDA team
Rhipsalidopsis sp., Schlumbergera sp.
-
activity increases during subculturing
Manually annotated by BRENDA team
B6V8F7, Q9T0N8
-
Manually annotated by BRENDA team
-
strong expression in kernel
Manually annotated by BRENDA team
B6V8F7, Q9T0N8
-
Manually annotated by BRENDA team
D3JAJ5
germinating
Manually annotated by BRENDA team
Triticum aestivum Yanyou 361
-
germinating
-
Manually annotated by BRENDA team
-
the vascular tissue and medullary region of sepals and petals show slight activity of CKX
Manually annotated by BRENDA team
-
floral whorls, not in calyx and corolla
Manually annotated by BRENDA team
-, Q84U27, Q8H6F6, Q94KI4
-
Manually annotated by BRENDA team
Q94KI5
developing, TaCKX6a
Manually annotated by BRENDA team
Q9T0N8
most abundant in
Manually annotated by BRENDA team
-, Q84U27, Q8H6F6, Q94KI4
young; young and adult
Manually annotated by BRENDA team
Q94KI5
Puccinia-infected: TaCKX2a
Manually annotated by BRENDA team
-
diurnal variation. Maximal activity 3 h after the dark-light transition and during the diminishing of the midday cytokinin peak
Manually annotated by BRENDA team
-
involvement of cytokinin oxidase/dehydrogenase in regulation of cytokinin levels in leaves
Manually annotated by BRENDA team
Rhipsalidopsis sp., Schlumbergera sp.
-
greenhouse-grown, enzyme activity increases during subculturing
Manually annotated by BRENDA team
A0MQ40
lower CK content and higher enzymatic activity in cv. Manuela leaves compared with cv. Scinado
Manually annotated by BRENDA team
-
first foliage leaves are cut of and kept in water in the darkness which results in a high increase in CKX activity
Manually annotated by BRENDA team
A2TLJ3, -
low level
Manually annotated by BRENDA team
-
35 kDa isozyme CKX35 in chlorotic leaves associated with a reduction in representative cytokinin levels and high H2O2 concentrations, and 37 kDa isozyme CKX37 in green leaves associated with representative CKs and H2O2 at normal levels
Manually annotated by BRENDA team
Q9T0N8
of shoots, most abundant in
Manually annotated by BRENDA team
B6V8F7, Q9T0N8
-
Manually annotated by BRENDA team
Q94KI5
young, TaCKX7a
Manually annotated by BRENDA team
-
major enzyme activity
Manually annotated by BRENDA team
A0MQ40
cv. Manuela has a higher endogenous cytokinin content and slightly lower enzyme activity than cv. Scinado
Manually annotated by BRENDA team
A0MQ40
highest activity in root nodule, very low enzyme level in R50 mutant compared with wild type
Manually annotated by BRENDA team
A2TLJ3, -
high level
Manually annotated by BRENDA team
-
isozyme AtCKX4
Manually annotated by BRENDA team
-
isozyme OsCKX1
Manually annotated by BRENDA team
-
isoyzme ZmCKX10
Manually annotated by BRENDA team
-
after removal of roots, no cytokinin oxidase activity remains after 24 h
Manually annotated by BRENDA team
Triticum aestivum Bashkirskaya 24
-
-
-
Manually annotated by BRENDA team
Triticum durum Bezenchukskaya 139
-
after removal of roots, no cytokinin oxidase activity remains after 24 h
-
Manually annotated by BRENDA team
Q94KI5
TaCKX1, TaCKX2b, developing, TaCKX6a/b
Manually annotated by BRENDA team
Q94KI5
TaCKX1 in drought-stressed seedlings, TaCKX3
Manually annotated by BRENDA team
Q9T0N8
phloem exudate
Manually annotated by BRENDA team
Triticum aestivum Bashkirskaya 24, Triticum durum Bezenchukskaya 139
-
-
-
Manually annotated by BRENDA team
Q9T0N8
most abundant in
Manually annotated by BRENDA team
A2TLJ3, -
high level in shoot apices
Manually annotated by BRENDA team
-
isozyme AtCKX4
Manually annotated by BRENDA team
-
isozyme OsCKX4
Manually annotated by BRENDA team
-
isozymes ZmCKX2 and ZmCKX10
Manually annotated by BRENDA team
Triticum aestivum Bashkirskaya 24
-
-
-
Manually annotated by BRENDA team
Q94KI5
Fusarium-infected: TaCKX5a/b and TaCKX6a
Manually annotated by BRENDA team
Q94KI5
at early flowering: TaCKX7b and TaCKX8, at late flowering: TaCKX4
Manually annotated by BRENDA team
B6V8F7, Q9T0N8
;
Manually annotated by BRENDA team
-
in the stalk of the leaf branched glandular trichomes, the CKX activity is restricted to the stalk region only, and is not present in the secretory head
Manually annotated by BRENDA team
Solanum lycopersicum Improved Pope
-
35 kDa isozyme CKX35 in chlorotic leaves associated with a reduction in representative cytokinin levels and high H2O2 concentrations, and 37 kDa isozyme CKX37 in green leaves associated with representative CKs and H2O2 at normal levels
-
Manually annotated by BRENDA team
additional information
-, Q84U27, Q8H6F6, Q94KI4
tissue specific and developmental expression of barley isozymes, overview
Manually annotated by BRENDA team
additional information
Q94KI5
tissue specific expression of wheat isozymes
Manually annotated by BRENDA team
additional information
Q9T0N8
tissue localization analysis
Manually annotated by BRENDA team
additional information
-
enzyme activity during vegetative development of plants
Manually annotated by BRENDA team
additional information
A2TLJ3, -
not detectable in stem
Manually annotated by BRENDA team
additional information
-
the enzyme activity is not detected in the epidermal layers of leaf, stem and root, but the epidermal outgrowth and the leaf trichome show prominent CKX activity
Manually annotated by BRENDA team
additional information
-
isozyme AtCKX1 is expressed in all plant organs
Manually annotated by BRENDA team
additional information
-
isozyme ZmCKX1 in reproductive organs
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
isozymes OsCKX1 and OsCKX4
Manually annotated by BRENDA team
-
isozymes ZmCKX1 and ZmCKX2
Manually annotated by BRENDA team
-, O22213, Q9LTS3
secreted isozymes
Manually annotated by BRENDA team
-
isozyme OsCKX11
Manually annotated by BRENDA team
B6V8F7, Q9T0N8
-
Manually annotated by BRENDA team
-
isozyme ZmCKX10
Manually annotated by BRENDA team
-, O22213, Q9LTS3
isozyme AtCKX7
Manually annotated by BRENDA team
-
secretion pathway, enzymes AtCKX2, and AtCKX3-AtCKX6
-
Manually annotated by BRENDA team
Q9FE45
secretion pathway
-
Manually annotated by BRENDA team
Q8H6F6
secretory pathway
-
Manually annotated by BRENDA team
-
secretion pathway, the glycosylated isozyme accumulates preferentially in the culture medium
-
Manually annotated by BRENDA team
-
secretion pathway, enzymes OsCKX1, and OsCKX3-OsCKX5
-
Manually annotated by BRENDA team
Q9T0N8
secretion pathway
-
Manually annotated by BRENDA team
-, Q84U27, Q8H6F6, Q94KI4
-
Manually annotated by BRENDA team
-
enzymes AtCKX1 and AtCKX3
Manually annotated by BRENDA team
-, Q84U27, Q8H6F6, Q94KI4
-
Manually annotated by BRENDA team
-, O22213, Q9LTS3
isozyme AtCKX1; isozyme AtCKX3
Manually annotated by BRENDA team
additional information
D3JAJ5
TaCKX3 has no signal peptide
-
Manually annotated by BRENDA team
additional information
Triticum aestivum Yanyou 361
-
TaCKX3 has no signal peptide
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25100
-
-
gel filtration
40000
60000
-
gel filtration
55000
94400
-
gel filtration
60000
-
-
non denaturing PAGE
64000
-
-
gel filtration, MALDI-MS
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 69000, recombinant enzyme, SDS-PAGE, x * 63000, native enzyme, SDS-PAGE
?
-, Q84U27, Q8H6F6, Q94KI4
x * 58100, HvCKX3, amino acid sequence calculation; x * 58800, HvCKX2, amino acid sequence calculation
?
-
x * 64900, AtCKX1, amino acid sequence calculation, x * 57400, AtCKX2, amino acid sequence calculation, x * 59400, AtCKX3, amino acid sequence calculation, x * 58100, AtCKX4, amino acid sequence calculation, x * 59900, AtCKX5, amino acid sequence calculation, x * 56500, AtCKX6, amino acid sequence calculation, x * 57900, AtCKX7, amino acid sequence calculation
?
Q9FE45
x * 60400, DsCKX1, amino acid sequence calculation
?
Q8H6F6
x * 58800, amino acid sequence calculation
?
-
x * 49800, amino acid sequence calculation
?
-
x * 59100, OsCKX1, amino acid sequence calculation, x * 56000, OsCKX2, amino acid sequence calculation, x * 58000, OsCKX3and OsCKX5, amino acid sequence calculation, x * 58400, OsCKX4, amino acid sequence calculation
?
-
x * 47900, amino acid sequence calculation
?
Q9T0N8
x * 57200, amino acid sequence calculation
?
A0MQ40
x * 57966, calculated from the deduced amino acid sequence
?
A2TLJ3, -
x * 60383, calculated from the deduced amino acid sequence
?
-, O22213, Q9LTS3
x * 60000, SDS-PAGE, x * 57057, mass spectrometry; x * 92000, SDS-PAGE, x * 59720, sequence calculation, x * 58080, mass spectrometry; x * 97000, SDS-PAGE, x * 65050, sequence calculation, x * 64290, mass spectrometry
?
D3JAJ5
x * 55700, about, sequence calculation
?
Triticum aestivum Yanyou 361
-
x * 55700, about, sequence calculation
-
monomer
-
1 * 60000, SDS-PAGE
monomer
-
1 * 5600, deglycosylated enzyme, SDS-PAGE
monomer
-
1 * 69865, MALDI-TOF MS, crystal structure analysis
monomer
Q9FUJ1
crystal structure analysis
additional information
-
x * 61000, SDS-PAGE, weak bands also at 75000 Da and100000 Da, x * 53300, calculated from the deduced amino acid sequence
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
-
enzyme amino acid sequences encoded by the 7 distinct genes in Arabidopsis contain 3 to 8 putative glycosylation sites, overview
glycoprotein
-
three N-glycosylation sites, glycosylation of the enzyme can explain the difference between theoretical and experimental weight
glycoprotein
Q9FE45
enzyme amino acid sequence contains 2 putative glycosylation sites
glycoprotein
-, Q84U27, Q8H6F6, Q94KI4
enzyme amino acid sequence contains 5 potential glycosylation sites
glycoprotein
Q8H6F6
enzyme amino acid sequence contains 5 putative glycosylation sites
glycoprotein
-
high increase in enzyme glycosylation rate during dark-treatment
glycoprotein
-
2 isozymes: 1 glycosylated, 1 non-glycosylated
glycoprotein
-
enzyme binds with moderate affinity to concanavalin A, glycosylation is influenced by cytokinins and has a regulatory role for the enzyme activity, glycosylation changes the enzyme secretion level
no glycoprotein
-
2 isozymes: 1 glycosylated, 1 non-glycosylated
glycoprotein
-
enzyme amino acid sequence contains 1 putative glycosylation site
glycoprotein
-
enzyme amino acid sequences encoded by the 5 distinct genes in rice contain 1-6 putative glycosylation sites
glycoprotein
Phaseolus sp.
-
2 isozymes: 1 glycosylated, 1 non-glycosylated
no glycoprotein
Phaseolus sp.
-
2 isozymes: 1 glycosylated, 1 non-glycosylated
glycoprotein
-
enzyme amino acid sequence contains 2 putative glycosylation sites
glycoprotein
Q9T0N8
enzyme amino acid sequence contains 5-8 putative glycosylation sites
glycoprotein
-
contains 22% neutral sugars
glycoprotein
-
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
sitting-drop vapor diffusion method
Q9FUJ1
hanging-drop vapor-diffusion method in the presence of N6-(buta-2,3-dienyl)adenine or N6-(penta-2,3-dienyl)adenine
-
purified free recombinant enzyme, or purified recombinant enzyme complexed with trans-zeatin, N6-isopentenyladenine, and N6-benzyladenine, sitting drop vapour diffusion method, 20C, protein solution: 30 mg/ml protein, 20 mM HEPES-KOH, pH 7.5, mixed with equal volume of well solution containing 0.1 M sodium acetat, pH 4.6, 0.2 M ammonium sulfate, and 12% w/v PEG 5000 monomethylether, a few hours, soaking in ligand solution and/or cryoprotectant 30% glycerol before data collection, X-ray diffraction structure determination and analysis at 1.7-2.0 A resolution, single isomorphous replacement, Fourier methods
-
purified recombinant enzyme, 20 mg/ml protein with 0.5% w/v n-octyl-beta-D-glucopyranoside, hanging drop vapour diffusion method, 0.002 ml protein solution mixed with 0.002 ml reservoir solution containing 30% w/v PEG 1500, 0.1 M Tris-HCl, pH 7.0, 1 week, 20C, X-ray diffraction structure determination and analysis at 1.95 A resolution
-
wild type and L492A mutant enzymes, hanging drop vapor diffusion method
-
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
55
-
-
T50 for 30 min incubation
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
recombinant protein
Q9FUJ1
recombinant protein from culture medium
-
recombinant vacuolar His-tagged isozyme AtCKX1 41.7fold from Pichia pastoris by ultrafiltration, hydrophobic interaction and hydroxyapatite chromatography, followed by nickel affinity chromatography and another step of hydrophobic interaction chromatography; recombinant vacuolar isozyme AtCKX3 18fold from Pichia pastoris by ultrafiltration, hydroxyapatite chromatography, nickel affinity chromatography, and anion exchange chromatography and another step of hydrophobic interaction chromatography
-, O22213, Q9LTS3
partially
-
ammonium sulfate precipitation
-
partial from leaves and calli of wild-type and transgenic plants
-
ammonium sulfate precipitation
-
ammonium sulfate precipitation
-
of recombinant protein
-
partially, recombinant enzyme from Pichia pastoris
-
recombinant from yeast Yarrowia lipolytica by ammonium sulfate fractionation, gel filtration, and ion exchange chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
7 distinct genes, AtCKX1-AtCKX7, DNA and amino acid sequence determination and analysis, functional expression of AtCKX2 and AtCKX4 in Pichia pastoris, Physcomitrella patens, and Saccharomyces cerevisiae, the recombinant enzymes are located in protoplasts or are secreted to the medium, overview, phylogenetic analysis
-
expressed as extracellular protein in Saccharomyces cerevisae
-
expressed in Physcomitrella patens protoplasts, expression of the AtCKX2 gene results in an up to 27fold increase of activity compared with wild type Physcomitrella patens, expression of AtCKX2 results in a significant decrease of cytokinin level and morphological changes such as altered morphology of filaments, impaired budding, and loss of sexual reproductivity
-
expression of cytosolic isozyme AtCKX7 in Escherichia coli and intracellularly in Pichia pastoris; expression of vacuolar His-tagged isozyme AtCKX1 in Pichia pastoris; expression of vacuolar isozyme AtCKX3 in Pichia pastoris
-, O22213, Q9LTS3
heterologously expressed in Saccharomyces cerevisiae
-
gene DsCKX1, DNA and amino acid sequence determination and analysis, analysis of expression, regulation, phylogenetic analysis
Q9FE45
expressed in Escherichia coli strain DH5alpha
Q94KI4
gene HvCKX1, DNA sequence determination and analysis; gene HvCKX2, DNA and amino acid sequence determination and analysis, expression in Saccharomyces cerevisiae, transformation and overexpression in Arabidopsis thaliana and Nicotiana tabacum using the Agrobacterium tumefaciens strain GUS3101 system; gene HvCKX3, DNA and amino acid sequence determination and analysis, expression in Saccharomyces cerevisiae, transformation and overexpression in Arabidopsis thaliana and Nicotiana tabacum using the Agrobacterium tumefaciens strain GUS3101 system
-, Q84U27, Q8H6F6, Q94KI4
gene HvCKX2, DNA and amino acid sequence determination and analysis, phylogenetic analysis
Q8H6F6
gene NsCKX1, DNA and amino acid sequence determination and analysis, phylogenetic analysis
-
5 distinct genes OsCKX1-OsCKX5, DNA and amino acid sequence determination and analysis, phylogenetic analysis
-
gene FasV of RfCKX1, expression as polycistronic mRNA, fas operon, DNA and amino acid sequence determination and analysis, phylogenetic analysis
-
expressed in Escherichia coli strain DH5alpha
-
gene TaCKX3, DNA and amino acid sequence determination and analysis, phylogenetic tree
D3JAJ5
semi-quantitative RT-PCR expression analysis
-
;
B6V8F7, Q9T0N8
expressed in Yarrowia lipolytica
-
expression in Pichia pastoris
-
expression of ZmCKX1 in Pichia pastoris
Q9T0N8
functional expression in Pichia pastoris
-
gene ZmCKX1, DNA and amino acid sequence determination and analysis, functional expression in Pichia pastoris, Physcomitrella patens, and Saccharomyces cerevisiae, phylogenetic analysis
Q9T0N8
high-level expression of cytokinin oxidase/dehydrogenase in Yarrowia lipolytica
-
overexpression in Pichia pastoris
-
overexpression in yeast Yarrowia lipolytica
-
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
highest levels of the CKX1 transcript are detected in spikes 0 days, 7 days, and 14 days after pollination and in the seedling roots
Q94KI4
light promotes an 8fold increase of cytokinin oxidase/dehydrogenase activity within the first 4 days of incubation under a light/dark regime of 18 h white light and 6 h darkness. The CKX activity reaches a high maximum when the plants have an age of 36 d
-
light promotes an increase of cytokinin oxidase/dehydrogenase activity within the first 4 days of incubation under a light/dark regime of 18 h white light and 6 h darkness
-
24-epibrassinolide inhibits both cytokinin oxidase activity and expression. Treatment with 0.4 mM 24-epibrassinolide leads to elevated cytokinin levels in roots and shoots, that are maintained only in the presence of 24-epibrassinolide, while the hormone removal leads to return of cytokinin concentration to the control level initially in the roots and then in the shoots
-
highest levels of the CKX1 transcript are detected in spikes 0 days, 7 days, and 14 days after pollination and in the seedling roots
-
24-epibrassinolide inhibits both cytokinin oxidase activity and expression. Treatment with 0.4 mM 24-epibrassinolide leads to elevated cytokinin levels in roots and shoots, that are maintained only in the presence of 24-epibrassinolide, while the hormone removal leads to return of cytokinin concentration to the control level initially in the roots and then in the shoots
Triticum aestivum Bashkirskaya 24
-
-
light promotes an increase of cytokinin oxidase/dehydrogenase activity within the first 4 days of incubation under a light/dark regime of 18 h white light and 6 h darkness
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
E381A
-
the mutation mimics the active site composition of naturally occurring isozymes CKO4/6 and CKO2/3
E381S
-
the mutation mimics the active site composition of naturally occurring isozymes CKO4/6 and CKO2/3
L492A
-
the mutant retains an unchanged pattern of selectivity to variously substituted ureas but it is much more susceptible to the inhibition by phenylureas as IC50 values are 10-50times lower than those for wild type enzyme
additional information
-
transgenic tobacco plants overexpressing ArCKX1, AtCKX5, or AtCKX6 shows increased enzyme activity and reduced cytokinin content phenotype, phenotypic effects of enzyme overexpression in transgenic Arabidopsis plants, overview
additional information
-
overexpression of the AtCKX genes in tobacco leading to shoot senescence, an increase of the root meristem zone, and enhanced formation of lateral roots
additional information
-, Q84U27, Q8H6F6, Q94KI4
overexpression of HvCKX3 in transgenic tobacco plants does not lead to increased enzyme activity or an altered phenotype; transgenic tobacco plants overexpressing HvCKX2 show a very strong phenotype of cytokinin deficiency with shorter interndes, highly increased formation of roots, and a dwarf growth habit, and died without producing seeds
additional information
-
phenotypic effects of enzyme overexpression in transgenic tobacco, overview
additional information
-
construction of transgenic tobacco plants conditionally overexpressing isopentenyltransferase IPT, tetracycline-induced derepressing of the IPT transcription, results in enhanced cytokinin oxidase activity
additional information
-
silencing of the OsCKX2 gene of rice cytokinin oxidase
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
agriculture
-
reduced expression of OsCKX2 causes cytokinin accumulation in inflorescence meristems and increases the number of reproductive organs, resulting in enhanced grain yield. Quantitative trait loci pyramiding to combine loci for grain number and plant heigh in the same genetic background generates lines exhibiting both beneficial traits. The results provide a strategy for tailormade crop improvement