Information on EC 1.5.99.12 - cytokinin dehydrogenase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.5.99.12
-
RECOMMENDED NAME
GeneOntology No.
cytokinin dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N6-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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redox reaction
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reduction
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-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Zeatin biosynthesis
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-
SYSTEMATIC NAME
IUBMB Comments
N6-dimethylallyladenine:acceptor oxidoreductase
A flavoprotein(FAD). Catalyses the oxidation of cytokinins, a family of N6-substituted adenine derivatives that are plant hormones, where the substituent is a dimethylallyl or other prenyl group. Although this activity was previously thought to be catalysed by a hydrogen-peroxide-forming oxidase, this enzyme does not require oxygen for activity and does not form hydrogen peroxide. 2,6-Dichloroindophenol, methylene blue, nitroblue tetrazolium, phenazine methosulfate and Cu(II) in the presence of imidazole can act as acceptors. This enzyme plays a part in regulating rice-grain production, with lower levels of the enzyme resulting in enhanced grain production [2].
CAS REGISTRY NUMBER
COMMENTARY hide
55326-39-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene DsCKX1 or DSCKX1
SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
no activity in Physcomitrella patens
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Manually annotated by BRENDA team
no activity in Pichia pastoris
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-
-
Manually annotated by BRENDA team
no activity in Prochlorococcus marinus
-
-
-
Manually annotated by BRENDA team
no activity in Raphanus sativus
-
-
-
Manually annotated by BRENDA team
no activity in Saccharomyces cerevisiae
-
-
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Manually annotated by BRENDA team
no activity in Synechocystis sp.
-
-
-
Manually annotated by BRENDA team
no activity in Synechocystis sp. PCC 6803
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-
-
Manually annotated by BRENDA team
i.e. Anabaena sp., PCC 7120, gene NsCKX1
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-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Phaseolus sp.
2 isozymes: 1 glycosylated, 1 non-glycosylated
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-
Manually annotated by BRENDA team
highest activity in one year old plants
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
radish, cv. Rampouch
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-
Manually annotated by BRENDA team
Rhipsalidopsis sp.
-
-
-
Manually annotated by BRENDA team
phytopathogen causing the witches broom disease, gene FasV of RfCKX1
-
-
Manually annotated by BRENDA team
Schlumbergera sp.
-
-
-
Manually annotated by BRENDA team
Solanum lycopersicum Improved Pope
-
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-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Triticum aestivum Bashkirskaya 24
-
-
-
Manually annotated by BRENDA team
Triticum aestivum Yanyou 361
CKX3; gene TaCKX3
UniProt
Manually annotated by BRENDA team
Triticum turgidum subsp. durum Bezenchukskaya 139
-
-
-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
Zea mays ZmCKX1
ZmCKX1
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the CKX gene family of Arabidopsis thaliana comprises seven members; the CKX gene family of Arabidopsis thaliana comprises seven members; the CKX gene family of Arabidopsis thaliana comprises seven members
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
cis-zeatin + acceptor + H2O
?
show the reaction diagram
cis-zeatin + electron acceptor
(2Z)-4-hydroxy-3-methylbut-2-enal + adenine + reduced electron acceptor
show the reaction diagram
cis-zeatin + oxidized 2,6-dichlorophenolindophenol
?
show the reaction diagram
cis/trans-zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
cis/trans-zeatin riboside + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
-
-
-
ir
cytokinin + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
? + reduced 2,3-dimethoxy-5-methyl-1,4-benzoquinone
show the reaction diagram
-
-
-
?
cytokinin riboside + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
? + reduced 2,3-dimethoxy-5-methyl-1,4-benzoquinone
show the reaction diagram
-
-
-
?
dihydrozeatin + electron acceptor
?
show the reaction diagram
-
-
-
-
?
isopentenyladenine + 2,6-dichlorophenolindophenol
adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
isopentenyladenine 9-glucoside + 2,6-dichlorophenolindophenol
adenine 9-glucoside + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
isopentenyladenosine + 2,6-dichlorophenolindophenol
adenosine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
isopentenyladenosine + acceptor + H2O
? + reduced acceptor
show the reaction diagram
isopentenyladenosine monophosphate + 2,6-dichlorophenolindophenol
adenosine monophosphate + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
kinetin + 2,3-dimethoxy-5-methyl-1,4-benzoquinone + H2O
?
show the reaction diagram
-
-
-
?
kinetin + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
low activity
-
-
?
kinetin + electron acceptor
2-furaldehyde + adenine + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
kinetin + FAD
2-furaldehyde + adenine + FADH2
show the reaction diagram
-
-
-
-
?
kinetin riboside + 2,3-dimethoxy-5-methyl-1,4-benzoquinone + H2O
?
show the reaction diagram
-
-
-
?
m-topolin + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
very low activity
-
-
?
N-benzyladenine + electron acceptor
benzaldehyde + adenine + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
N-methyl-isopentenyladenine + FAD
?
show the reaction diagram
-
-
-
-
?
N6-(2-butynyl)adenine + 2,6-dichlorophenol indophenol
?
show the reaction diagram
-
very effective electron acceptor
-
-
?
N6-(2-isopentenyl)adenine + 1,4-naphthoquinone
?
show the reaction diagram
-
very effective electron acceptor
-
-
?
N6-(2-isopentenyl)adenine + 1,4-naphthoquinone
adenine + 3-methylbut-2-enal + reduced 1,4-naphthoquinone
show the reaction diagram
-
-
-
?
N6-(2-isopentenyl)adenine + 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical + H2O
adenine + 3-methylbut-2-enal + ?
show the reaction diagram
-
-
-
-
?
N6-(2-isopentenyl)adenine + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
adenine + 3-methylbut-2-enal + reduced 2,3-dimethoxy-5-methyl-1,4-benzoquinone
show the reaction diagram
i.e. coenzyme Q0
-
-
?
N6-(2-isopentenyl)adenine + 2,3-dimethoxy-5-methyl-1,4-benzoquinone + H2O
adenine + 3-methylbut-2-enal + 2,3-dimethoxy-5-methyl-1,4-benzoquinol
show the reaction diagram
-
4-nitrosoresorcinol-1-monomethyl ether serves as a weak electron acceptor of CKX
-
-
?
N6-(2-isopentenyl)adenine + 2,3-dimethoxy-5-methyl-1,4-benzoquinone + H2O
adenine + 3-methylbut-2-enal + ?
show the reaction diagram
-
-
-
?
N6-(2-isopentenyl)adenine + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
N6-(2-isopentenyl)adenine + 2,6-dichlorophenol indophenol
?
show the reaction diagram
N6-(2-isopentenyl)adenine + 2,6-dichlorophenolindophenol
adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
N6-(2-isopentenyl)adenine + 2,6-dichlorophenolindophenol + H2O
adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
N6-(2-isopentenyl)adenine + 4-nitrosoresorcinol-1-monomethyl ether + H2O
adenine + 3-methylbut-2-enal + reduced 4-nitrosoresorcinol-1-monomethyl ether
show the reaction diagram
-
4-nitrosoresorcinol-1-monomethyl ether serves as a weak electron acceptor of CKX
-
-
?
N6-(2-isopentenyl)adenine + acceptor + H2O
?
show the reaction diagram
-
preferred substrate of isozyme AtCKX4
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-
?
N6-(2-isopentenyl)adenine + caffeic acid
adenine + 3-methylbut-2-enal + ?
show the reaction diagram
-
-
-
?
N6-(2-isopentenyl)adenine + coenzyme Q1
adenine + 3-methylbut-2-enal + reduced coenzyme Q1
show the reaction diagram
-
-
-
?
N6-(2-isopentenyl)adenine + CuCl2
?
show the reaction diagram
N6-(2-isopentenyl)adenine + CuCl2 + H2O
adenine + 3-methylbut-2-enal + ?
show the reaction diagram
N6-(2-isopentenyl)adenine + cytochrome c
adenine + 3-methylbut-2-enal + reduced cytochrome c
show the reaction diagram
-
-
-
?
N6-(2-isopentenyl)adenine + daphnoretin
adenine + 3-methylbut-2-enal + ?
show the reaction diagram
-
-
-
?
N6-(2-isopentenyl)adenine + duroquinone
?
show the reaction diagram
-
low activity
-
-
?
N6-(2-isopentenyl)adenine + electron acceptor
adenine + 3-methylbut-2-enal + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
N6-(2-isopentenyl)adenine + FAD
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
N6-(2-isopentenyl)adenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
-
-
-
-
ir
N6-(2-isopentenyl)adenine + hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one + H2O
adenine + 3-methylbut-2-enal + reduced hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one
show the reaction diagram
-
CKX1 is able to use hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one as a poor electron acceptor at neutral pH levels, but not in acidic pH levels
-
-
?
N6-(2-isopentenyl)adenine + o-coumaric acid
adenine + 3-methylbut-2-enal + ?
show the reaction diagram
-
-
-
?
N6-(2-isopentenyl)adenine + O2
?
show the reaction diagram
-
low activity
-
-
?
N6-(2-isopentenyl)adenine + O2
adenine + 3-methylbut-2-enal + H2O2
show the reaction diagram
oxidative degradation of cytokinins, slow reaction, low substrate specificity
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-
?
N6-(2-isopentenyl)adenine + plantacyanine
adenine + 3-methylbut-2-enal + reduced plantacyanine
show the reaction diagram
electron acceptor from spinach
-
-
?
N6-(2-isopentenyl)adenine + potassium ferricyanide
?
show the reaction diagram
-
low activity
-
-
?
N6-(2-isopentenyl)adenine + rosmarinic acid
adenine + 3-methylbut-2-enal + ?
show the reaction diagram
-
-
-
?
N6-(2-isopentenyl)adenine + scopoletin
adenine + 3-methylbut-2-enal + ?
show the reaction diagram
-
-
-
?
N6-(2-isopentenyl)adenine + vitamin K1
adenine + 3-methylbut-2-enal + reduced vitamin K1
show the reaction diagram
-
-
-
?
N6-(2-isopentenyl)adenine + vitamin K3
?
show the reaction diagram
-
low activity
-
-
?
N6-(2-isopentenyl)adenine + vitamin K3
adenine + 3-methylbut-2-enal + reduced vitamin K3
show the reaction diagram
-
-
-
?
N6-(2-isopentenyl)adenine 9-beta-D-glucoside + 3',5'-dimethoxy-4'-hydroxyacetophenone + H2O
adenine 9-beta-D-glucoside + 3-methylbut-2-enal + ?
show the reaction diagram
i.e. iP9G, best substrate for isozyme AtCKX1 at pH 5.0, 3',5'-dimethoxy-4-hydroxyacetophenone is the best acceptor
-
-
?
N6-(2-isopentenyl)adenine 9-beta-D-riboside + 2,3,5-triphenyl-tetrazolium chloride + H2O
adenine 9-beta-D-riboside + 3-methylbut-2-enal + ?
show the reaction diagram
-
-
-
?
N6-(2-isopentenyl)adenine 9-beta-D-riboside + 2,3-dimethoxy-5-methyl-1,4-benzoquinone + H2O
adenine 9-beta-D-riboside + 3-methylbut-2-enal + ?
show the reaction diagram
-
-
-
?
N6-(2-isopentenyl)adenine 9-beta-D-riboside + 3',5'-dimethoxy-4'-hydroxyacetophenone + H2O
adenine 9-beta-D-riboside + 3-methylbut-2-enal + ?
show the reaction diagram
-
-
-
?
N6-(2-isopentenyl)adenine 9-beta-D-riboside + ferricyanide + H2O
adenine 9-beta-D-riboside + 3-methylbut-2-enal + ferrocyanide
show the reaction diagram
-
-
-
?
N6-(2-isopentenyl)adenine 9-beta-D-riboside + ferricytochrome c + H2O
adenine 9-beta-D-riboside + 3-methylbut-2-enal + ferrocytochrome c
show the reaction diagram
-
-
-
?
N6-(2-isopentenyl)adenine 9-beta-D-riboside + NAD+ + H2O
adenine 9-beta-D-riboside + 3-methylbut-2-enal + ?
show the reaction diagram
-
-
-
?
N6-(2-isopentenyl)adenine 9-beta-D-riboside + O2 + H2O
adenine 9-beta-D-riboside + 3-methylbut-2-enal + ?
show the reaction diagram
-
-
-
?
N6-(2-isopentenyl)adenine 9-beta-D-riboside + oxidized 2,6-dichlorophenolindophenol + H2O
adenine 9-beta-D-riboside + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
N6-(2-isopentenyl)adenine 9-beta-D-riboside 5'-phosphate + 2,3-dimethoxy-5-methyl-1,4-benzoquinone + H2O
adenine 9-beta-D-riboside 5'-phosphate + 3-methylbut-2-enal + ?
show the reaction diagram
-
-
-
?
N6-(2-isopentenyl)adenine 9-glucoside + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
low activity
-
-
?
N6-(2-isopentenyl)adenine 9-riboside + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
N6-(2-isopentenyl)adenine 9-riboside-5'-monophosphate + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
low activity
-
-
?
N6-(2-isopentenyl)adenose + 2,6-dichlorophenolindophenol
adenosine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
N6-(2-isopentenyl)adenosine + 2,6-dichlorophenol indophenol
?
show the reaction diagram
-
very effective electron acceptor
-
-
?
N6-(2-isopentenyl)adenosine + FAD
adenosine + 3-methylbut-2-enal + FADH2
show the reaction diagram
-
-
-
?
N6-(4-hydroxy-2-butynyl)adenine + 2,6-dichlorophenol indophenol
?
show the reaction diagram
-
very effective electron acceptor
-
-
?
N6-(DELTA2-isopentenyl)adenine + 2,6-dichlorophenolindophenol + H2O
adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
artificial electron acceptor
-
-
?
N6-(DELTA2-isopentenyl)adenine + 4-methylcatechol + H2O
adenine + 3-methylbut-2-enal + reduced 4-methylcatechol
show the reaction diagram
-
-
-
?
N6-(DELTA2-isopentenyl)adenine + acceptor + H2O
adenine + 3-methylbut-2-enal + reduced acceptor
show the reaction diagram
-
-
-
-
?
N6-(DELTA2-isopentenyl)adenine + acetosyringone + H2O
adenine + 3-methylbut-2-enal + reduced acetosyringone
show the reaction diagram
-
-
-
?
N6-(DELTA2-isopentenyl)adenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
N6-(DELTA2-isopentenyl)adenine + guaiacol + H2O
adenine + 3-methylbut-2-enal + reduced guaiacol
show the reaction diagram
-
-
-
?
N6-(DELTA2-isopentenyl)adenosine + 2,6-dichlorophenolindophenol + H2O
adenosine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
artificial electron acceptor
-
-
?
N6-(DELTA2-isopentenyl)adenosine + 4-methylcatechol + H2O
adenosine + 3-methylbut-2-enal + reduced 4-methylcatechol
show the reaction diagram
-
-
-
?
N6-(DELTA2-isopentenyl)adenosine + acetosyringone + H2O
adenosine + 3-methylbut-2-enal + reduced acetosyringone
show the reaction diagram
-
-
-
?
N6-(DELTA2-isopentenyl)adenosine + FAD + H2O
adenosine + 3-methylbut-2-enal + FADH2
show the reaction diagram
-
-
-
?
N6-(DELTA2-isopentenyl)adenosine + guaiacol + H2O
adenosine + 3-methylbut-2-enal + reduced guaiacol
show the reaction diagram
-
-
-
?
N6-benzyladenine + electron acceptor
?
show the reaction diagram
-
-
-
-
?
N6-benzyladenine + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
N6-dimethylallyladenine + 2,6-dichlorophenolindophenol
adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
i.e. isopentenyladenine, reaction via an imine intermediate
-
-
?
N6-dimethylallyladenine + acceptor + H2O
adenine + 3-methylbut-2-enal + reduced acceptor
show the reaction diagram
N6-dimethylallyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
N6-dimethylallyladenine + FAD + H2O2
adenine + 3-methylbut-2-enal + FADH2 + O2
show the reaction diagram
-
i.e. isopentenyladenine
-
-
?
N6-dimethylallyladenosine + FAD + H2O
adenosine + 3-methylbut-2-enal + FADH2
show the reaction diagram
i.e. isopentenyladenosine
-
-
ir
N6-hexyladenine + electron acceptor
hexanal + adenine + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
N6-isopentenyl-2-(2-hydroxyethylamino)-9-methyladenine + electron acceptor
3-methyl-2-butenal + 2-hydroxyethylamino-9-methyladenine + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
N6-isopentenyl-2-methylthioadenine + electron acceptor
3-methyl-2-butenal + 2-methylthioadenine + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
N6-isopentenyladenine + 2,6-dichlorophenolindophenol
adenine + 3-methyl-2-butenal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
N6-isopentenyladenine + CuCl2
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
-
-
-
?
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
N6-isopentenyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
N6-isopentenyladenine + oxidized 2,6-dichlorophenolindophenol
adenine + 3-methylbut-2-enol + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
N6-isopentenyladenine 9-beta-D-glucoside + oxidized 2,6-dichlorophenolindophenol
adenine 9-beta-D-glucoside + 3-methylbut-2-enol + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
N6-isopentenyladenosine + 2,6-dichlorophenolindophenol
3-methylbut-2-enal + adenosine + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
-
N6-isopentenyladenosine + electron acceptor
3-methylbut-2-enal + adenosine + reduced electron acceptor
show the reaction diagram
N6-isopentenyladenosine + oxidized 2,6-dichlorophenolindophenol
adenosine + 3-methylbut-2-enol + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
N6-methyl-isopentenyladenine + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
very low activity
-
-
?
O-beta-D-glucosyl-trans-zeatin + oxidized 2,6-dichlorophenolindophenol
?
show the reaction diagram
-
-
-
?
o-topolin + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
very low activity
-
-
?
p-topolin + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
very low activity
-
-
?
p-topolin + FAD
?
show the reaction diagram
-
-
-
-
?
thidiazuron + electron acceptor
?
show the reaction diagram
-
-
-
-
?
trans-zeatin + 2,3-dimethoxy-5-methyl-1,4-benzoquinone + H2O
?
show the reaction diagram
-
-
-
?
trans-zeatin + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
very effective substrate
-
-
?
trans-zeatin + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
cis-zeatin is utilized only by some tissue-specific forms but not by HvCKX2
-
-
?
trans-zeatin + acceptor + H2O
?
show the reaction diagram
trans-zeatin + electron acceptor
(2E)-4-hydroxy-3-methylbut-2-enal + adenine + reduced electron acceptor
show the reaction diagram
-
-
-
-
?
trans-zeatin + FAD + H2O
? + FADH2
show the reaction diagram
trans-zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
trans-zeatin + oxidized 2,6-dichlorophenolindophenol
?
show the reaction diagram
trans-zeatin 9-glucoside + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
low activity
-
-
?
trans-zeatin 9-riboside + 2,3-dimethoxy-5-methyl-p-benzoquinone
?
show the reaction diagram
-
very effective substrate
-
-
?
trans-zeatin O-glucoside + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
trans-zeatin riboside + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
trans-zeatin riboside O-glucoside + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
trans-zeatin ribotide + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
trans-zeatin-9-glucoside + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
zeatin + 2,6-dichlorophenolindophenol
4-hydroxy-3-methylbut-2-enal + adenine + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
zeatin + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
zeatin + electron acceptor
4-hydroxy-3-methylbut-2-enal + adenine + reduced electron acceptor
show the reaction diagram
zeatin + FAD
? + FADH2
show the reaction diagram
-
-
-
?
zeatin + FAD + H2O
adenine + 4-hydroxy-3-methylbut-2-enal + FADH2
show the reaction diagram
-
-
-
-
ir
zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
zeatin riboside + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cis-zeatin + acceptor + H2O
?
show the reaction diagram
-
-
-
-
?
cis/trans-zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
Q9T0N8
enzyme catalyzes the irreversible degradation of cytokinins
-
-
ir
N6-(2-isopentenyl)adenine + FAD
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
Q9T0N8
the natural terminal electron acceptor probably is a p-quinone or a similar compound, high substrate specificity for N6-(2-isopentenyl)adenine
-
-
?
N6-(2-isopentenyl)adenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
-
-
-
-
ir
N6-(DELTA2-isopentenyl)adenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
N6-(DELTA2-isopentenyl)adenosine + FAD + H2O
adenosine + 3-methylbut-2-enal + FADH2
show the reaction diagram
Q9T0N8
-
-
-
?
N6-dimethylallyladenine + acceptor + H2O
adenine + 3-methylbut-2-enal + reduced acceptor
show the reaction diagram
N6-dimethylallyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
N6-isopentenyladenine + electron acceptor
adenine + 3-methyl-2-butenal + reduced electron acceptor
show the reaction diagram
A2TLJ3
-
-
-
ir
N6-isopentenyladenine + FAD + H2O
adenine + 3-methylbut-2-enal + FADH2
show the reaction diagram
Q9T0N8
oxidative degradation
-
-
ir
trans-zeatin + acceptor + H2O
?
show the reaction diagram
trans-zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
Q9FUJ3
enzyme catalyzes the irreversible degradation of cytokinins
-
-
ir
zeatin + electron acceptor
4-hydroxy-3-methylbut-2-enal + adenine + reduced electron acceptor
show the reaction diagram
A2TLJ3
-
-
-
ir
zeatin + FAD + H2O
adenine + 4-hydroxy-3-methylbut-2-enal + FADH2
show the reaction diagram
-
-
-
-
ir
zeatin + FAD + H2O
adenine + ? + FADH2
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,6-dichlorophenolindophenol
-
in vitro 4000fold faster reaction than with the native acceptor
flavin
additional information
enzyme shows a preference for quinones as electron acceptors
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2,6-dichloro-pyridin-4-yl)-carbamic acid benzyl ester
-
-
(2-chloro-pyridin-4-yl)-carbamic acid 4-chloro-phenyl ester
-
-
1-(2-chloro-1-oxidopyridin-4-yl)-3-phenylurea
-
-
1-imidazo[1,2-a]pyridin-7-yl-3-phenylurea
-
-
2,4-Dichlorophenoxyacetic acid
-
inhibits the enzyme by up to 31%
2-amino-6-(3-methoxyanilino)purine
-
-
2-chloro-6-(2-hydroxyanilino)purine
-
-
2-chloro-6-(2-methoxyanilino)purine
-
-
2-chloro-6-(3-chloroanilino)purine
-
-
2-chloro-6-(3-fluoroanilino)purine
-
-
2-chloro-6-(3-hydroxyanilino)purine
-
-
2-chloro-6-(3-methoxyanilino)-9-isopropylpurine
-
-
2-chloro-6-(3-methoxyanilino)-9-methylpurine
-
-
2-chloro-6-(3-methoxyanilino)purine
-
-
2-chloro-6-(4-chloroanilino)purine
-
-
2-chloro-6-(4-fluoroanilino)purine
-
-
2-chloro-6-(4-hydroxyanilino)purine
-
-
2-chloro-6-(4-methoxyanilino)purine
-
-
2-chloro-6-anilinopurine
-
-
2-fluoro-6-(3-methoxyanilino)purine
-
-
2-methylthio-6-(3-methoxyanilino)purine
-
-
2-nitro-6-(3-methoxyanilino)purine
-
-
24-epibrassinolide
-
inhibits both cytokinin oxidase activity and expression. Treatment with 0.4 mM 24-epibrassinolide leads to elevated cytokinin levels in roots and shoots, that are maintained only in the presence of 24-epibrassinolide, while the hormone removal leads to return of cytokinin concentration to the control level initially in the roots and then in the shoots
6-(3-methoxyanilino)purine
-
-
Acriflavine
-
-
adenine
-
slight product inhibition
alpha-naphthaleneacetic acid
-
inhibits the enzyme by up to 54%
cordycepin
-
-
diphenylene iodonium
-
-
diphenylurea
; competitive
N-(1,2,3-thidiazol-5-yl)-N'-phenylurea
-
-
N-(2,6-dichloro-pyridin-4-yl)-N'-benzylurea
-
-
N-(2,6-dichloro-pyridin-4-yl)-N'-phenoxyurea
-
-
N-(2,6-dichloro-pyridin-4-yl)-N'-phenylurea
-
-
N-(2,6-dichloropyridin-4-yl)-N'-benzyl-N'-methylurea
-
-
-
N-(2-amino-6-chloro-pyridin-4-yl)-N'-benzylurea
-
-
N-(2-amino-6-chloro-pyridin-4-yl)-N'-phenylurea
-
one of the most potent inhibitors of CKO
N-(2-amino-pyridin-4-yl)-N'-phenylurea
-
one of the most potent inhibitors of CKO
N-(2-chloro-4-pyridyl)-N'-phenylurea
-
-
N-(2-chloro-4-pyridyl-N'-5-fluoro-phenylurea)
-
-
N-(2-chloro-6-methoxy-pyridin-4-yl)-N'-benzylurea
-
-
N-(2-chloro-pyridin-4-yl)-N'-benzylurea
-
-
N-(2-chloro-pyridin-4-yl)-N'-phenylurea
-
the compound binds in a planar conformation and competes for the same binding site with natural substrates
N6-(2-bromo-2-isopentenyl)adenine
-
weak inhibition
N6-(2-butynyl)adenine
-
irreversible inhibition
N6-(3-hydroxy-2-phenylethyl)-2-(3-hydroxypropylamino)-9-isopentenyladenine
-
-
N6-(4-hydroxy-2-butynyl)adenine
-
irreversible inhibition
N6-(buta-2,3-dienyl)adenine
-
strong irreversible mechanism based inhibition
N6-(penta-2,3-dienyl)adenine
-
strong irreversible mechanism based inhibition
N6-benzyl-2-(2-hydroxyethylamino)-9-mehtyladenine
-
-
N6-benzyl-2-amino-9-isoproyladenine
-
-
N6-benzyladenine
-
strongly stimulates enzyme activity by up to 180%
N6-but-2,3-dienyl-aminopurine
-
mechanism-based irreversible (suicide) inhibitor. IC50: 0.02 mM with N6-(2-isopentenyl)adenine as substrate, 0.0004 mM with N6-(2-isopentenyl)adenosine as substrate and less than 0.0001 mM with zeatin as substrate
N6-isopentenyl-N-methylaminopurine
; structural analogue of isopentenyladenine
N6-pent-2,3-dienyl aminopurine
-
10%, 52%, and 86% inhibition at concentrations of 0.001 mM, 0.01 mM and 0.1 mM
phenylhydrazine
-
-
Quinacrine
-
-
rifampicin
-
-
thidiazuron
trans-zeatin
-
enhances enzyme activity by 35%
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one
-
-
imidazole
-
in complex with Cu2+ enhances activity
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.067
2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical
-
pH 5.0, temperature not specified in the publication
0.0041 - 0.192
4-nitrosoresorcinol-1-monomethyl ether
0.046
cis-zeatin
pH 7.0
0.0089 - 0.25
hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one
0.092
N6-(2-butynyl)adenine
-
37C
0.0011 - 0.0032
N6-(2-isopentenyl)adenine
0.38
N6-(4-hydroxy-2-butynyl)adenine
-
37C
0.001 - 0.0025
N6-(DELTA2-isopentenyl)adenine
0.0015 - 0.054
N6-dimethylallyladenine
0.0011 - 0.003
N6-isopentenyladenine
0.012 - 0.015
N6-isopentenyladenosine
0.006 - 0.06
trans-zeatin
0.011
trans-zeatin riboside
pH 7.0
0.009
zeatin
-
30C, pH 7.4
0.063
zeatin riboside
-
30C, pH 7.4
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
169.6
1,4-Naphthoquinone
Zea mays
Q9T0N8
pH 6.5, recombinant enzyme
143.6
2,3-dimethoxy-5-methyl-1,4-benzoquinone
Zea mays
Q9T0N8
pH 6.5, recombinant enzyme
178.4 - 278
2,6-dichlorophenolindophenol
0.3
caffeic acid
Zea mays
Q9T0N8
pH 6.5, recombinant enzyme
123.1
coenzyme Q1
Zea mays
Q9T0N8
pH 6.5, recombinant enzyme
0.8
cytochrome c
Zea mays
Q9T0N8
pH 6.5, recombinant enzyme
1
daphnoretin
Zea mays
Q9T0N8
pH 6.5, recombinant enzyme
1.9
kinetin
Zea mays
-
pH 6.5, 25C
0.13
N-methyl-isopentenyladenine
Zea mays
-
pH 6.5, 25C
5.4 - 143.6
N6-(2-isopentenyl)adenine
129.8
N6-(2-isopentenyl)adenosine
Zea mays
Q9T0N8
pH 6.5, recombinant enzyme, with cofactor 2,3-dimethoxy-5-methyl-1,4-benzoquinone
128
N6-isopentenyladenine
Zea mays
-
30C, pH 7.4
62
N6-isopentenyladenosine
Zea mays
-
30C, pH 7.4
0.3
o-coumaric acid
Zea mays
Q9T0N8
pH 6.5, recombinant enzyme
0.1 - 0.6
O2
0.23
p-topolin
Zea mays
-
pH 6.5, 25C
0.4
rosmarinic acid
Zea mays
Q9T0N8
pH 6.5, recombinant enzyme
1.4
scopoletin
Zea mays
Q9T0N8
pH 6.5, recombinant enzyme
0.9
spinach plantacyanine
Zea mays
Q9T0N8
pH 6.5, recombinant enzyme
-
2.3
vitamin K1
Zea mays
Q9T0N8
pH 6.5, recombinant enzyme
85.4
vitamin K3
Zea mays
Q9T0N8
pH 6.5, recombinant enzyme
135
zeatin
Zea mays
-
30C, pH 7.4
21
zeatin riboside
Zea mays
-
30C, pH 7.4
additional information
additional information
Zea mays
Q9T0N8
at pH above pH 6.5 polymerization of the quinone acceptors occur
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
N6-(buta-2,3-dienyl)adenine
-
-
0.026
N6-(penta-2,3-dienyl)adenine
-
-
0.012
N6-isopentenyl-N-methylaminopurine
recombinant enyme
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.004 - 0.009
(2,6-dichloro-pyridin-4-yl)-carbamic acid benzyl ester
0.0002 - 0.039
(2-chloro-pyridin-4-yl)-carbamic acid 4-chloro-phenyl ester
0.005 - 0.13
1-(2-chloro-1-oxidopyridin-4-yl)-3-phenylurea
0.0001 - 0.006
1-imidazo[1,2-a]pyridin-7-yl-3-phenylurea
0.027
2-amino-6-(3-methoxyanilino)purine
Arabidopsis thaliana
-
-
0.0039
2-chloro-6-(3-chloroanilino)purine
Arabidopsis thaliana
-
-
0.0075
2-chloro-6-(3-fluoroanilino)purine
Arabidopsis thaliana
-
-
0.00375
2-chloro-6-(3-hydroxyanilino)purine
Arabidopsis thaliana
-
-
0.061
2-chloro-6-(3-methoxyanilino)-9-methylpurine
Arabidopsis thaliana
-
-
0.0019
2-chloro-6-(3-methoxyanilino)purine
Arabidopsis thaliana
-
-
0.0254
2-chloro-6-(4-chloroanilino)purine
Arabidopsis thaliana
-
-
0.05
2-chloro-6-(4-fluoroanilino)purine
Arabidopsis thaliana
-
-
0.08
2-chloro-6-anilinopurine
Arabidopsis thaliana
-
-
0.001
2-fluoro-6-(3-methoxyanilino)purine
Arabidopsis thaliana
-
-
0.014
2-nitro-6-(3-methoxyanilino)purine
Arabidopsis thaliana
-
-
0.0124
6-(3-methoxyanilino)purine
Arabidopsis thaliana
-
-
0.005 - 0.05
N-(1,2,3-thidiazol-5-yl)-N'-phenylurea
0.002 - 0.009
N-(2,6-dichloro-pyridin-4-yl)-N'-benzylurea
0.005 - 0.02
N-(2,6-dichloro-pyridin-4-yl)-N'-phenoxyurea
0.00008 - 0.0045
N-(2,6-dichloro-pyridin-4-yl)-N'-phenylurea
0.0011 - 0.002
N-(2,6-dichloropyridin-4-yl)-N'-benzyl-N'-methylurea
0.006 - 0.032
N-(2-amino-6-chloro-pyridin-4-yl)-N'-benzylurea
0.00004 - 0.0018
N-(2-amino-6-chloro-pyridin-4-yl)-N'-phenylurea
0.002 - 0.035
N-(2-amino-pyridin-4-yl)-N'-phenylurea
0.006 - 0.022
N-(2-chloro-6-methoxy-pyridin-4-yl)-N'-benzylurea
0.0015 - 0.018
N-(2-chloro-pyridin-4-yl)-N'-benzylurea
0.035 - 0.055
N-(2-chloro-pyridin-4-yl)-N'-phenylurea
0.02 - 2
N6-but-2,3-dienyl-aminopurine
Solanum tuberosum
-
mechanism-based irreversible (suicide) inhibitor. IC50: 0.02 mM with N6-(2-isopentenyl)adenine as substrate, 0.0004 mM with N6-(2-isopentenyl)adenosine as substrate and less than 0.0001 mM with zeatin as substrate
0.029
thidiazuron
Arabidopsis thaliana
-
-
additional information
additional information
Arabidopsis thaliana
-
IC50-values above 0.1 mM for 2-chloro-6-(2-hydroxyanilino)purine, 2-chloro-6-(4-hydroxyanilino)purine, 2-chloro-6-(2-methoxyanilino)purine, 2-chloro-6-(4-methoxyanilino)purine, 2-methylthio-6-(3-methoxyanilino)purine and 2-chloro-6-(3-methoxyanilino)-9-isopropylpurine, no inhibition observed for 6-anilinopurine and trans-zeatin
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0003
-
transgenic plant conditionally overexpressing isopentenyltransferase, cell culture medium, not induced; wild-type plant, cell extract and cell culture medium, not induced or tetracycline-treated cells
0.0004
-
transgenic plant conditionally overexpressing isopentenyltransferase, cell extract, not induced cells
0.002
-
wild-type plant and transgenic plant conditionally overexpressing isopentenyltransferase, cell extract, N6-benzylaminopurine-induced
0.004
-
transgenic plant conditionally overexpressing isopentenyltransferase, cell culture medium of N6-benzylaminopurine-induced cells
0.005
-
wild-type plant, cell culture medium of N6-benzylaminopurine-induced cells
0.0052
-
N6-(2-isopentenyl)adenine + 2,3-dimethoxy-5-methyl-p-benzoquinone, pH 7.0, 37C
0.0065
-
transgenic plant conditionally overexpressing isopentenyltransferase, cell culture medium of tetracycline-treated cells
30.6
-
purified recombinant enzyme
62.4
-
-
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5
native overall enzyme activity in grains and leaves shows 2 maxima at pH 4.5 and at pH 7.0-7.5; recombinant HvCKX2 expressed in tobacco, native overall enzyme activity in grains and leaves shows 2 maxima at pH 4.5 and at pH 7.0-7.5
5 - 7.5
assay at; assay at; assay at
5 - 7
-
-
7 - 7.5
-
with 2,3-dimethoxy-5-methyl-p-benzoquinone
7 - 7.5
7.1
-
assay at
8
-
with 2,6-dichlorophenol indophenol
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10
-
pH 6.0: about 45% of maximal activity, pH 10.0: aout 55% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
-
assay at room temperature
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.9
-
calculated from sequence
5.93
sequence calculation
6
sequence calculation
6.25
-
chromatofocusing
6.6
calculated from the deduced amino acid sequence
7.1
sequence calculation
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
of kernels, most abundant in
Manually annotated by BRENDA team
-
the vascular tissue and medullary region of sepals and petals show slight activity of CKX
Manually annotated by BRENDA team
-
floral whorls, not in calyx and corolla
Manually annotated by BRENDA team
of shoots, most abundant in
Manually annotated by BRENDA team
-
isozyme OsCKX11
Manually annotated by BRENDA team
TaCKX1, TaCKX2b, developing, TaCKX6a/b
Manually annotated by BRENDA team
Fusarium-infected: TaCKX5a/b and TaCKX6a
Manually annotated by BRENDA team
at early flowering: TaCKX7b and TaCKX8, at late flowering: TaCKX4
Manually annotated by BRENDA team
-
in the stalk of the leaf branched glandular trichomes, the CKX activity is restricted to the stalk region only, and is not present in the secretory head
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
of guard cells
Manually annotated by BRENDA team
additional information