Information on EC 1.5.99.12 - cytokinin dehydrogenase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
1.5.99.12
-
RECOMMENDED NAME
GeneOntology No.
cytokinin dehydrogenase
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Zeatin biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
N6-dimethylallyladenine:acceptor oxidoreductase
A flavoprotein(FAD). Catalyses the oxidation of cytokinins, a family of N6-substituted adenine derivatives that are plant hormones, where the substituent is a dimethylallyl or other prenyl group. Although this activity was previously thought to be catalysed by a hydrogen-peroxide-forming oxidase, this enzyme does not require oxygen for activity and does not form hydrogen peroxide. 2,6-Dichloroindophenol, methylene blue, nitroblue tetrazolium, phenazine methosulfate and Cu(II) in the presence of imidazole can act as acceptors. This enzyme plays a part in regulating rice-grain production, with lower levels of the enzyme resulting in enhanced grain production [2].
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
AtCKX2
-
seven distinct genes AtCKX1 to AtCKX7, AtCKX2 is subject of the study
AtCKX2
Q9FUJ3
-
AtCKX3
Q9LTS3
-
CKO
Triticum durum Bezenchukskaya 139
-
-
-
CKO
-
-
CKO1
-
-
CKX
Q84U27
-
CKX
Q8H6F6
-
CKX
Nostoc sp., Oryza sativa, Phaseolus sp.
-
-
CKX
Solanum lycopersicum Improved Pope
-
-
-
CKX
D3JAJ5
-
CKX
Triticum aestivum Bashkirskaya 24
-
-
-
CKX
Triticum aestivum Yanyou 361
D3JAJ5
-
-
CKX1
Q94KI4
-
CKX1
-
-
CKX3
D3JAJ5
-
CKX3
Triticum aestivum Yanyou 361
D3JAJ5
-
-
cytokinin dehydrogenase
-
-
cytokinin dehydrogenase CKX1
Q9T0N8
-
cytokinin dehydrogenase CKX10
B6V8F7
-
cytokinin oxidase
-
-
-
-
cytokinin oxidase
-
-
cytokinin oxidase
Q9T0N8
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
Q9FUJ3
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
Q9FE45
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
Q8H6F6
-
cytokinin oxidase/dehydrogenase
Q94KI4
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
Nostoc sp., Oryza sativa, Phaseolus sp.
-
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
A0MQ40
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
Rhipsalidopsis sp.
-
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
Schlumbergera sp.
-
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
D3JAJ5
-
cytokinin oxidase/dehydrogenase
Q94KI5
-
cytokinin oxidase/dehydrogenase
Triticum aestivum Bashkirskaya 24
-
-
-
cytokinin oxidase/dehydrogenase
Triticum aestivum Yanyou 361
D3JAJ5
-
-
cytokinin oxidase/dehydrogenase
-
-
cytokinin oxidase/dehydrogenase
Q9T0N8
-
HvCKX1
-
-
N6-(D2-isopentenyl)adenosine oxidase
-
-
-
-
N6-isopentenylaldenine oxidase
-
-
-
-
OsCKX2
-
-
PsCKX1
A0MQ40
-
PsCKX2
A0MQ40
-
zeatin oxidase
-
-
-
-
ZmCKO1
-
-
ZmCKO1
Q9T0N8
-
ZmCKX1
-
-
isopentenyladenosine oxidase
-
-
-
-
additional information
Q9FUJ3
formerly EC 1.4.3.18
additional information
-
formerly EC 1.4.3.18
additional information
-
see also EC 1.4.3.18
additional information
-
formerly EC 1.4.3.18
additional information
-
enzyme belongs to the CKX gene family
additional information
Q9T0N8
enzyme belongs to the plant CKX family
CAS REGISTRY NUMBER
COMMENTARY
55326-39-1
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
isozyme AtCKX1
UniProt
Manually annotated by BRENDA team
isozyme AtCKX3
UniProt
Manually annotated by BRENDA team
isozyme AtCKX7
-
-
Manually annotated by BRENDA team
isozymes AtCKX1, AtCKX4, and AtCKX7
-
-
Manually annotated by BRENDA team
L., Heynh. variety Columbia
-
-
Manually annotated by BRENDA team
cv. Cocozelle
-
-
Manually annotated by BRENDA team
gene DsCKX1 or DSCKX1
SwissProt
Manually annotated by BRENDA team
cultivar Alexis
-
-
Manually annotated by BRENDA team
cultivars Golden Promise, Scarlett, Torka, and Kontesa
SwissProt
Manually annotated by BRENDA team
cv Amulet
-
-
Manually annotated by BRENDA team
cv. Luxor, gene HvCKX1, barley contains at least 7 different gene homologues
SwissProt
Manually annotated by BRENDA team
cv. Luxor, gene HvCKX2, barley contains at least 7 different gene homologues
SwissProt
Manually annotated by BRENDA team
cv. Luxor, gene HvCKX3, barley contains at least 7 different gene homologues
SwissProt
Manually annotated by BRENDA team
cv. Luxor, gene HvCKX7, genomic DNA, barley contains at least 7 different gene homologues, gene HvCKX7, genomic DNA
-
-
Manually annotated by BRENDA team
gene and isozyme HvCKX1
-
-
Manually annotated by BRENDA team
gene HvCKX2
SwissProt
Manually annotated by BRENDA team
L., cv. Alexis
-
-
Manually annotated by BRENDA team
2 isozymes: 1 glycosylated, 1 non-glycosylated
-
-
Manually annotated by BRENDA team
cv Wisconsin 38
-
-
Manually annotated by BRENDA team
cv. Wisconsin 38, wild-type, and transgenic tobacco conditionally overexpressing isopentenyltransferase IPT
-
-
Manually annotated by BRENDA team
L. cv. Wisconsin 38
-
-
Manually annotated by BRENDA team
no activity in Physcomitrella patens
-
-
-
Manually annotated by BRENDA team
no activity in Pichia pastoris
-
-
-
Manually annotated by BRENDA team
no activity in Prochlorococcus marinus
-
-
-
Manually annotated by BRENDA team
no activity in Raphanus sativus
-
-
-
Manually annotated by BRENDA team
no activity in Saccharomyces cerevisiae
-
-
-
Manually annotated by BRENDA team
no activity in Synechocystis sp.
-
-
-
Manually annotated by BRENDA team
no activity in Synechocystis sp. PCC 6803
-
-
-
Manually annotated by BRENDA team
i.e. Anabaena sp., PCC 7120, gene NsCKX1
-
-
Manually annotated by BRENDA team
5 distinct genes OsCKX1-OsCKX5
-
-
Manually annotated by BRENDA team
isozymes OsCKX1, OsCKX4, and OsCKX11
-
-
Manually annotated by BRENDA team
Phaseolus sp.
2 isozymes: 1 glycosylated, 1 non-glycosylated
-
-
Manually annotated by BRENDA team
cv Great Northern
-
-
Manually annotated by BRENDA team
Hedw., wild type and transgenic cells expressing the AtCKX2 gene from Arabidopsis thaliana, expression of AtCKX2 results in a significant decrease of cytokinin level and morphological changes such as altered morphology of filaments, impaired budding, and loss of sexual reproductivity
-
-
Manually annotated by BRENDA team
highest activity in one year old plants
-
-
Manually annotated by BRENDA team
cv. Scinado and cv. Manuela
-
-
Manually annotated by BRENDA team
L. cv. Gotik
-
-
Manually annotated by BRENDA team
L. cv. Scinado and cv. Manuela, cv. Scinado normally requires shorter growing period than cv. Manuela
UniProt
Manually annotated by BRENDA team
PsCKX1; L. cv. Sparkle, wild type and R50 carrying a recessive sym16 which is a pleiotropic mutant of pea which develops few, pale nodules and has pale young leaves, R50 has reduced CKX activity and increased cytokinin level compared with wild type, sequencing of DNA from both strains did not show any obvious mutations within the gene
UniProt
Manually annotated by BRENDA team
radish, cv. Rampouch
-
-
Manually annotated by BRENDA team
Rhipsalidopsis sp.
-
-
-
Manually annotated by BRENDA team
phytopathogen causing the witches broom disease, gene FasV of RfCKX1
-
-
Manually annotated by BRENDA team
Schlumbergera sp.
-
-
-
Manually annotated by BRENDA team
Solanum lycopersicum Improved Pope
-
-
-
Manually annotated by BRENDA team
CKX3; gene TaCKX3
UniProt
Manually annotated by BRENDA team
cultivar Wanad
-
-
Manually annotated by BRENDA team
cv Samantha
-
-
Manually annotated by BRENDA team
cv. Samantha, gene TaCKX1, wheat contains at least 8 different gene homologues
SwissProt
Manually annotated by BRENDA team
Triticum aestivum Bashkirskaya 24
-
-
-
Manually annotated by BRENDA team
Triticum aestivum Yanyou 361
CKX3; gene TaCKX3
UniProt
Manually annotated by BRENDA team
Triticum durum Bezenchukskaya 139
-
-
-
Manually annotated by BRENDA team
cv. Cellux 225
SwissProt
Manually annotated by BRENDA team
enzyme and gene ZmCKX1
SwissProt
Manually annotated by BRENDA team
enzyme ZmCKO1
-
-
Manually annotated by BRENDA team
gene ZmCKX1
SwissProt
Manually annotated by BRENDA team
isoyzmes ZmCKX1, ZmCKX2, and ZmCKX10
-
-
Manually annotated by BRENDA team
variety Cellux 225
-
-
Manually annotated by BRENDA team
ZmCKX1
SwissProt
Manually annotated by BRENDA team
Zea mays ZmCKX1
ZmCKX1
SwissProt
Manually annotated by BRENDA team
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.01
-
N6-(buta-2,3-dienyl)adenine
-
-
0.026
-
N6-(penta-2,3-dienyl)adenine
-
-
0.012
-
N6-isopentenyl-N-methylaminopurine
Q9T0N8
recombinant enyme
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
55
-
-
T50 for 30 min incubation