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EC Tree
IUBMB Comments Also catalyses the reaction of EC 1.5.1.17 alanopine dehydrogenase, but more slowly. Does not act on L-strombine.
The enzyme appears in viruses and cellular organisms
Synonyms
strombine dehydrogenase, strombine/alanopine dehydrogenase,
more
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Dehydrogenase, strombine
-
-
-
-
STRDH
Paraprionospio pinnata
-
-
strombine/alanopine dehydrogenase
St/AlDH functions physiologically as strombine dehydrogenase
SDH
-
-
-
-
StDH
-
-
-
-
StDH
shows 68% sequence identity with the tauropine dehydrogenase protein from the marine demosponge Halichondria japonica
strombine dehydrogenase
-
-
strombine dehydrogenase
-
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N-(carboxymethyl)-D-alanine + NAD+ + H2O = glycine + pyruvate + NADH + H+
-
-
-
-
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N-(carboxymethyl)-D-alanine:NAD+ oxidoreductase (glycine-forming)
Also catalyses the reaction of EC 1.5.1.17 alanopine dehydrogenase, but more slowly. Does not act on L-strombine.
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Ala + pyruvate + NADH
meso-alanopine + NAD+ + H2O
Paraprionospio pinnata
-
-
-
-
?
beta-Ala + pyruvate + NADH
?
-
-
-
-
?
Gly + 2-ketobutyrate + NADH + H+
2-[(carboxymethyl)amino]butanoic acid + NAD+ + H2O
8.9% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
-
-
?
Gly + 2-oxobutanoate + NADH
?
Gly + 2-oxopentanoate + NADH
?
-
19.6% of the activity with pyruvate
-
-
?
Gly + glyoxylate + NADH
?
Gly + glyoxylate + NADH + H+
[(carboxymethyl)amino]propanedioic acid + NAD+ + H2O
3.9% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
-
-
?
Gly + hydroxypyruvate + NADH
?
-
29.5% of the activity with pyruvate
-
-
?
Gly + oxaloacetate + NADH
?
-
106% of the activity with pyruvate
-
-
?
Gly + oxaloacetate + NADH + H+
N-carboxymethylglycine + NAD+ + H2O
39.1% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
Gly + pyruvate + NADH + H+
D-strombine + NAD+ + H2O
94.6% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
-
-
?
Gly + pyruvate + NADPH
D-strombine + NADP+ + H2O
less than 0.5% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
-
-
?
glycine + pyruvate + NADH
D-strombine + NAD+ + H2O
glycine + pyruvate + NADH + H+
N-(carboxymethyl)-D-alanine + NAD+ + H2O
-
the reduction reaction is preferred
-
-
r
L-2-Aminobutanoate + pyruvate + NADH
?
L-2-Aminobutyrate + pyruvate + NADH
?
-
-
-
-
?
L-Ala + 2-ketobutyrate + NADH + H+
2-[[(1S)-1-carboxyethyl]amino]butanoic acid + NAD+ + H2O
8.9% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
-
-
?
L-Ala + glyoxylate + NADH + H+
N-(carboxymethyl)-L-Ala + NAD+ + H2O
4.0% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
-
-
?
L-Ala + oxaloacetate + NADH + H+
[[(1S)-1-carboxyethyl]amino]propanedioic acid + NAD+ + H2O
40.0% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
-
-
?
L-Ala + pyruvate + NADH
?
-
Gly and Ala are preferred substrates
-
-
?
L-Ala + pyruvate + NADH
meso-Alanopine + NAD+ + H2O
L-Ala + pyruvate + NADH + H+
meso-alanopine + NAD+ + H2O
100% activity
-
-
?
L-Ala + pyruvate + NADPH
meso-alanopine + NADP+ + H2O
less than 0.5% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
-
-
?
L-Cys + pyruvate + NADH
?
-
19.9% of the activity with Gly
-
-
?
L-Cys + pyruvate + NADH + H+
N-(1-carboxyethyl)-L-Cys + NAD+ + H2O
1.7% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
-
-
?
L-Met + pyruvate + NADH
?
-
3.7% of the activity with Gly
-
-
?
L-Ser + pyruvate + NADH
?
L-Ser + pyruvate + NADH + H+
N-(1-carboxyethyl)-L-Ser + NAD+ + H2O
14.2% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
-
-
?
L-Thr + pyruvate + NADH
?
L-Val + pyruvate + NADH
?
-
11.2% of the activity with Gly
-
-
?
L-Val + pyruvate + NADH + H+
N-(1-carboxyethyl)-L-Val + NAD+ + H2O
0.9% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
-
-
?
additional information
?
-
-
the enzyme functions as the terminal dehydrogenase of glycolysis and has a role in maintaining energy production under the stresses of environmental or functional anoxia
-
-
?
Gly + 2-oxobutanoate + NADH
?
-
at 24.8% of the activity with pyruvate
-
-
?
Gly + 2-oxobutanoate + NADH
?
-
30% of the activity with pyruvate
-
-
?
Gly + glyoxylate + NADH
?
-
23.6% of the activity with pyruvate
-
-
?
Gly + glyoxylate + NADH
?
-
20% of the activity with pyruvate
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
Balanus cariosus
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
r
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
Scolelepis fuliginosa
-
-
-
-
?
Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
the enzyme is highly specific to glycine. No enzyme activity is observed when other amino acids (alanine and taurine) are used as substrates in the experiment
-
-
?
glycine + pyruvate + NADH
D-strombine + NAD+ + H2O
-
Gly and Ala are preferred substrates
-
-
?
glycine + pyruvate + NADH
D-strombine + NAD+ + H2O
enzyme is specific for glycine
-
-
?
L-2-Aminobutanoate + pyruvate + NADH
?
-
68.3% of the activity with Gly
-
-
?
L-2-Aminobutanoate + pyruvate + NADH
?
-
at 48% of the activity with Gly
-
-
?
L-Ala + pyruvate + NADH
meso-Alanopine + NAD+ + H2O
-
101% of the activity with Gly
-
-
?
L-Ala + pyruvate + NADH
meso-Alanopine + NAD+ + H2O
-
at 76% of the activity with Gly
-
-
?
L-Ala + pyruvate + NADH
meso-Alanopine + NAD+ + H2O
-
r
-
?
L-Ala + pyruvate + NADH
meso-Alanopine + NAD+ + H2O
-
94% of the activity with Gly
-
-
?
L-Ala + pyruvate + NADH
meso-Alanopine + NAD+ + H2O
-
in the reverse reaction: 81% of the activity with meso-alanopine compared to strombine
-
?
L-Ser + pyruvate + NADH
?
-
88.5% of the activity with Gly
-
-
?
L-Ser + pyruvate + NADH
?
-
at 82% of the activity with Gly
-
-
?
L-Ser + pyruvate + NADH
?
-
-
-
-
?
L-Ser + pyruvate + NADH
?
-
31% of the activity with Gly
-
-
?
L-Thr + pyruvate + NADH
?
-
9.3% of the activity with Gly
-
-
?
L-Thr + pyruvate + NADH
?
-
-
-
-
?
L-Thr + pyruvate + NADH
?
-
3.7% of the activity with Gly
-
-
?
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glycine + pyruvate + NADH + H+
N-(carboxymethyl)-D-alanine + NAD+ + H2O
-
the reduction reaction is preferred
-
-
r
additional information
?
-
-
the enzyme functions as the terminal dehydrogenase of glycolysis and has a role in maintaining energy production under the stresses of environmental or functional anoxia
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NAD+
-
-
NAD+
Scolelepis fuliginosa
-
-
NAD+
Balanus cariosus
-
-
NAD+
Paraprionospio pinnata
-
-
NAD+
-
no activity with NADP+
NADH
-
-
NADH
Scolelepis fuliginosa
-
-
NADH
Balanus cariosus
-
-
NADH
-
no activity with NADPH
NADH
enzyme shows an absolute requirement for NADH
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Ca2+
-
10% of enzymatic activity is inhibited at 10 mM
Cu2+
-
50% of enzymatic activity is inhibited at 1 mM
Fe3+
-
inhibits enzyme at 0.2 mM
Mg2+
-
20% of enzymatic activity is inhibited at 10 mM
Mn2+
-
13% of enzymatic activity is inhibited at 1 mM
Zn2+
-
inhibits enzyme at 0.6 mM
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pyruvate
inhibited by an excess of substrate pyruvate; substrate inhibition, in excess, pyruvate enters in competition with the other substrate (glycine and/or NADH) preventing the site of reaction to take all the substrates to proceed with the enzymatic reaction. Its optimal concentration is determined to be round 2.0-2.3 mM
additional information
-
the enzyme activity in adductor muscle decreases following the marine-brackish gradient
-
D-lactate
-
weak
L-lactate
-
weak
meso-Alanopine
-
-
succinate
-
weak
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additional information
-
increased activity of alanopine dehydrogenase, ADH, and strombine dehydrogenase, SDH, enzymes in Montipora capitata exposed to prolonged durations of hypoxic conditions
-
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11.71 - 12.77
2-Ketobutyrate
2.46 - 3.55
2-oxobutanoate
196
beta-Ala
-
pH 7.5, 25°C
9.17
D-strombine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
34.2
glycine
-
pH 7.5, 25°C
44 - 457
L-2-aminobutanoate
45.9
L-2-aminobutyrate
-
pH 7.5, 25°C
9.07
meso-Alanopine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
11.71
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
12.77
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
2.46
2-oxobutanoate
-
-
36.5
Gly
-
-
40.1
Gly
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
42.4
Gly
Scolelepis fuliginosa
-
-
4.18
glyoxylate
-
-
44
L-2-aminobutanoate
-
-
457
L-2-aminobutanoate
-
-
39.2
L-Ala
-
pH 7.5, 25°C
39.8
L-Ala
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
163
L-Ser
-
pH 7.5, 25°C
382.1
L-Ser
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
48
L-Thr
-
-
0.85
NAD+
apparent value, isozyme St/AlDH1, with 20 mM D-strombine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
0.87
NAD+
apparent value, isozyme St/AlDH1, with 20 mM meso-alanopine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
0.07
NADH
apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
0.07
NADH
apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
1.7
oxaloacetate
-
-
10.2
oxaloacetate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
10.2
oxaloacetate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
0.32
pyruvate
-
-
0.34
pyruvate
pH 7.0, 30°C
0.34
pyruvate
determined using constant cosubstrate concentrations (100 mM glycine and 0.3 mM NADH, pH 7.0)
0.5
pyruvate
Scolelepis fuliginosa
-
-
0.96
pyruvate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
0.97
pyruvate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
1.39
pyruvate
-
pH 7.5, 25°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
21.77 - 23.2
2-Ketobutyrate
4.99
D-strombine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
50.08
Gly
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
51.7
L-Ala
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
23.64
L-Ser
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
5.12
meso-Alanopine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
42.66 - 44.25
oxaloacetate
21.77
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
23.2
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
3.86
NAD+
apparent value, isozyme St/AlDH1, with 20 mM D-strombine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
3.96
NAD+
apparent value, isozyme St/AlDH1, with 20 mM meso-alanopine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
43.58
NADH
apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
44.7
NADH
apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
42.66
oxaloacetate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
44.25
oxaloacetate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
47.33
pyruvate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
47.78
pyruvate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.63 - 1.35
iminodiacetate
3.69
acetate
-
pH 7.5, 25°C
5.57
acetate
-
pH 6.5, 25°C
16.4
D-lactate
-
pH 6.5, 25°C
17
D-lactate
-
pH 7.5, 25°C
0.63
iminodiacetate
-
pH 7.5, 25°C
1.35
iminodiacetate
-
pH 6.5, 25°C
21.2
L-lactate
-
pH 6.5, 25°C
22.9
L-lactate
-
pH 7.5, 25°C
6.93
oxaloacetate
-
pH 7.5, 25°C
14.3
oxaloacetate
-
pH 6.5, 25°C
3.98
succinate
-
pH 6.5, 25°C
15.4
succinate
-
pH 7.5, 25°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2.6
crude extract, at 30°C
87
after 29fold purification, at 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6 - 6.5
-
with Gly and pyruvate as substrates
7
-
assay at
7
assay at and optimal pH
7.2
-
assay at
7.2
-
with Gly and pyruvate as substrates
9
-
-
9
-
with strombine as substrate
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
25
-
assay at
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
45 - 46
-
temperature optimum
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
weak
-
-
brenda
-
-
-
brenda
Balanus cariosus
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
UniProt
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
from Hawaii
-
-
brenda
-
-
-
brenda
-
-
-
brenda
no activity in Aplysia juliana
-
-
-
brenda
no activity in Aplysia kurodai
-
-
-
brenda
no activity in Aurelia aurita
-
-
-
brenda
no activity in Cellana grata
-
-
-
brenda
no activity in Halocynthia roretzi
-
-
-
brenda
no activity in Hexagrammos otakii
-
-
-
brenda
no activity in Liolophura japonica
-
-
-
brenda
no activity in Loligo bleekeri
-
-
-
brenda
no activity in Octopus membranaceus
-
-
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brenda
no activity in Oncorhynchus keta
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no activity in Pollicipes mitella
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no activity in Pugettia quadridens
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no activity in Solaster paxillatus
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no activity in Stichopus japonicus
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no activity in Strongylocentrotus nudus
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no activity in Todarodes pacificus
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Paraprionospio pinnata
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collected in the straits of Messina area (central Mediterranean) between May and June of 2011, from two nearby marine and brackish-water sites, two populations
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Scolelepis fuliginosa
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UniProt
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the adductor muscle also shows a conspicuous amount of strombine dehydrogenase
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very low enzyme content
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additional information
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enzyme tissue distribution and expression analysis
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muscle
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muscle
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Scolelepis fuliginosa
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Balanus cariosus
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physiological function
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enzyme SDH acts as one of the primary and anaerobic metabolic responses pathways during hypoxia. Montipora capitata increasingly relies upon alanopine dehydrogenase, ADH, and strombine dehydrogenase, SDH, for anaerobic catabolism under low-oxygen conditions
metabolism
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bivalves have evolved diverse and highly specialised strategies for surviving in hypoxic episodes including pathways that are efficient both in terms of ATP production, and in minimising H+ and toxic end product accumulation. Under these circumstances, glycogen is metabolized to pyruvate and the cytosolic NADH/NAD+ redox ratio is balanced by the reduction of pyruvate to lactate. Alternatively, NAD+ can be recycled more efficiently by coupling an amino acid to pyruvate, with formation of opines such as alanopine, tauropine, octopine, and strombine. Specimens utilizing the octopine rather than the alanopine pathway will increase energy flow rapidly, developing a major ability to counteract environmental variations. The high ratio between malate dehydrogenase/lactate dehydrogenase is due to the ability of Pinna nobilis to turn on anaerobic metabolism as a consequence of environmental or anthropogenic stresses. Anaerobic pathways are not all equivalent in terms of energy production based upon maximum rates for ATP output (lactate > octopine > alanopine = strombine)
metabolism
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the enzyme activities of opine dehydrogenases, including strombine dehydrogenase, are increased during anaerobic metabolism in corals under prolonged oxygen deprivation, due to invasive algal overgrowth and oxygen deprivation of wide-spread distributed Montipora capitata in Kaneohe Bay, Oahu, Hawaii
additional information
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comparisons of opine dehydrogenases activities (octopine dehydrogenase, alanopine dehydrogenase, strombine dehydrogenase, and tauropine dehydrogenase) in the adductor muscle, overview. The ODH activity in adductor muscle increases following the marine-brackish gradient, while the one of ADH, SDH and TDH decreases following the same gradient
additional information
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enzyme SDH activity varies significantly with respect to hypoxia treatment type, treatment duration, and interaction between treatment duration and type. Importantly, control and tank samples display no significant differences over 3 h, 6 h, 1 day, and 3 day times sets, indicating that bubbling within small chambers has no effect on target enzyme activity
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B5D5Q1_AREMA
98
0
10389
TrEMBL
other Location (Reliability: 1 )
B7FBJ2_AREMA
400
0
43023
TrEMBL
other Location (Reliability: 4 )
B5D5Q0_CRAGI
387
0
43169
TrEMBL
other Location (Reliability: 2 )
B5D5P3_OSTED
405
0
44672
TrEMBL
other Location (Reliability: 2 )
B5D5P1_SIPNU
398
0
43659
TrEMBL
other Location (Reliability: 3 )
A0A9I9_MARSA
399
0
43478
TrEMBL
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B1GT63_SUBDO
337
0
36924
TrEMBL
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40600
reduced and denatured enzyme, estimated by SDS-PAGE
42000
native enzyme, estimated by SDS-PAGE
43350
deduced from amino acid sequence from strombine/alanopine dehydrogenase
35000
native PAGE
75000
native PAGE
75000
semi-native PAGE two bands at 35 kDa and 75 kDa are visible
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dimer
native PAGE and semi-native PAGE, 2 * 35000 Da
dimer
native and semi-native PAGE analysis, 2 * 35000
monomer
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monomer
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gel filtration, SDS-PAGE analysis, 1 * 46000
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56
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enzyme is heat labile, pretreatment at 56°C for 20 sec only retains 20% of its original activity
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4°C, glycerol, 2 weeks, 100% activity
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4°C, stable for at least 1 week
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ammonium sulfate precipitation, DEAE-cellulose SH column chromatography, hydroxyapatite column chromatography, and Toyopearl Super Q-650S column chromatography
purified over 470fold using ammonium sulfate fractionation, sephacryl S-100 chromatography, hydroxyapatite adsorption, DEAE-sepharose chromatography, Blue-sepharose chromatography
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partial
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expressed in Escherichia coli
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diagnostics
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the significant positive relationship between exposure length and enzyme activity of alanopine dehydrogenase, ADH, and SDH in Montipora capitata supports the implementation of these enzymes as biomarkers for rapid analysis of hypoxia-induced stress in corals
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Sato, M.; Takeuchi, M.; Kanno, N.; Nagahisa, E.; Sato, Y.
Distribution of opine dehydrogenases and lactate dehydrogenase activities in marine animals
Comp. Biochem. Physiol. B
106
955-960
1993
Anthopleura japonica, Anthopleura nigrescens, Asterias amurensis, Patiria pectinifera, Balanus cariosus, Buccinum isaotakii, Azumapecten farreri nipponensis, Chlorostoma lischkei, Crassostrea gigas, Fusitriton oregonensis, Halichondria japonica, Haliotis discus hannai, Hemigrapsus sanguineus, Littorina brevicula, Meretrix lusoria, Mytilus edulis, Neptunea arthritica, no activity in Aplysia kurodai, no activity in Aplysia juliana, no activity in Aurelia aurita, no activity in Cellana grata, no activity in Halocynthia roretzi, no activity in Hexagrammos otakii, no activity in Liolophura japonica, no activity in Loligo bleekeri, no activity in Octopus membranaceus, no activity in Oncorhynchus keta, no activity in Pollicipes mitella, no activity in Pugettia quadridens, no activity in Solaster paxillatus, no activity in Stichopus japonicus, no activity in Strongylocentrotus nudus, no activity in Todarodes pacificus, Octopus vulgaris, Pagurus samuelis, Mizuhopecten yessoensis, Perinereis nuntia, Pseudopotamilla occelata, Reishia clavigera, Ruditapes philippinarum, Anadara broughtonii, Scelidotoma gigas, Pseudocardium sachalinense
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Blackstock, J.; Burdass, M.C.
Pyruvate oxidoreductase in some sublittoral polychaetes
Biochem. Soc. Trans.
15
383-384
1987
Capitella capitata, Glycera alba, Scolelepis fuliginosa
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Fields, J.H.A.; Storey, K.B.
Tissue-specific alanopine dehydrogenase from the gill and strombine dehydrogenase from the foot muscle of the cherrystone clam Mercenaria mercenaria
J. Exp. Mar. Biol. Ecol.
105
175-185
1987
Mercenaria mercenaria
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Dando, P.R.
Strombine [N-(carboxymethyl)-D-alanine]dehydrogenase and alanopine [meso-N-(1-carboxyethyl)-alanine]dehydrogenase from the mussel Mytilus edulis L.
Biochem. Soc. Trans.
9
297-298
1981
Mytilus edulis
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Storey, K.B.
Tissue-specific alanopine dehydrogenase and strombine dehydrogenase from the sea mouse, Aphrodite aculeata (polychaeta)
J. Exp. Zool.
225
369-378
1983
Aphrodita aculeata
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Endo, N.; Kan-No, N.; Nagahisa, E.
Purification, characterization, and cDNA cloning of opine dehydrogenases from the polychaete rockworm Marphysa sanguinea
Comp. Biochem. Physiol. B
147B
293-307
2007
Marphysa sanguinea (A0A9I9)
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Quiroga, E.; Quinones, R.A.; Gonzalez, R.R.; Gallardo, V.A.; Jessen, G.
Aerobic and anaerobic metabolism of Paraprionospio pinnata (Polychaeta: Spionidae) in central Chile
J. Mar. Biolog. Assoc. UK
87
459-463
2007
Paraprionospio pinnata
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Plese, B.; Schroeder, H.C.; Grebenjuk, V.A.; Wegener, G.; Brandt, D.; Natalio, F.; Mueller, W.E.
Strombine dehydrogenase in the demosponge Suberites domuncula: characterization and kinetic properties of the enzyme crucial for anaerobic metabolism
Comp. Biochem. Physiol. B
154
102-107
2009
Suberites domuncula (B1GT63), Suberites domuncula
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Lee, A.C.; Lee, K.T.; Pan, L.Y.
Purification and kinetic characteristics of strombine dehydrogenase from the foot muscle of the hard clam (Meretrix lusoria)
Comp. Biochem. Physiol. B
158
38-45
2010
Meretrix lusoria
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Lagana, G.; Barreca, D.; Giacobbe, S.; Bellocco, E.
Anaerobiosis and metabolic plasticity of Pinna nobilis biochemical and ecological features
Biochem. Syst. Ecol.
56
138-143
2014
Pinna nobilis
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Murphy, J.W.; Richmond, R.H.
Changes to coral health and metabolic activity under oxygen deprivation
PeerJ
4
e1956
2016
Montipora capitata
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