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Information on EC 1.4.3.2 - L-amino-acid oxidase and Organism(s) Homo sapiens

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EC Tree
     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.2 L-amino-acid oxidase
IUBMB Comments
A flavoprotein (FAD).
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This record set is specific for:
Homo sapiens
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
laao, il4i1, l-amino-acid oxidase, l-aao, escapin, head kidney and gill, dolabellanin, l-phenylalanine oxidase, akbu-laao, m-lao, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aromatic L-amino acid oxidase
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IL4I1
Il4i1 protein
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secreted L-phenylalanine oxidase, physiologically expressed by myeloid cells, gene expression in primary mediastinal B-cell lymphoma
Interleukin Four Induced Gene 1 protein
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first described in the mouse, characterized in human B cells, expression is restricted to lymphoid tissues and induced by interleukin-4
L-amino acid oxidase
L-amino acid:O2 oxidoreductase
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L-aminooxidase
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L-phenylalanine oxidase
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LAO
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ophio-amino-acid oxidase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidative deamination
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redox reaction
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SYSTEMATIC NAME
IUBMB Comments
L-amino-acid:oxygen oxidoreductase (deaminating)
A flavoprotein (FAD).
CAS REGISTRY NUMBER
COMMENTARY hide
9000-89-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
L-tryptophan + H2O + O2
3-(1H-indol-3-yl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
L-tryptophan + H2O + O2
3-(1H-indol-3-yl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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most primary mediastinal B-cell lymphoma (89%) are positive for enzyme immunostaining with strong intracytoplasmic staining in up to 100% of tumor cells, CD19(+) and CD19(-) cells display enzyme activity
Manually annotated by BRENDA team
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64% positive immunostaining, no correlation between staining and histological grade of the follicular lymphoma, in small lymphocytic lymphoma 40% positive, restricted to paraimmunoblasts of proliferative centers, high enzyme activity, 7fold higher than in spleen lysate
Manually annotated by BRENDA team
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variable percentage of positive staining in several tissues, solid tumors, interfollicular areas, sinuses of reactive lymph nodes, red pulp of lymphoid follicles, thymus medulla, all lymphoma subtypes, particularly high enzyme expression in classical Hodgkin lymphoma and nodular lymphocyte predominant Hodgkin lymphoma cells
Manually annotated by BRENDA team
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42% positive staining of classical Hodgkin lymphoma in Hodgkin and Reed Sternberg cells, 91% positive staining of nodular lymphocyte predominant Hodgkin lymphoma cells
Manually annotated by BRENDA team
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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IL41I inhibits bacterial growth. The antibiotic action of IL4I1 is mainly due to H2O2 and NH3 production whereas its L-amino acid oxidase activity does not sufficiently deplete the medium of Phe or Trp to inhibit bacterial growth
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
OXLA_HUMAN
567
0
62881
Swiss-Prot
Secretory Pathway (Reliability: 1)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
whole cell lysates of viable cryopreserved lymph node cells from follicular lymphomas, spelnocytes from marginal zone lymphoma, and traumatic normal spleens, and cell lines or cells are submitted to magnetic cell sorting using anti-human CD19 MACS beads
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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a high level of enzyme expression seems associated with absence of bone marrow involvement and better outcome in follicular lymphoma cases, enzyme levels could serve as prognosis factor
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Carbonnelle-Puscian, A.; Copie-Bergman, C.; Baia, M.; Martin-Garcia, N.; Allory, Y.; Haioun, C.; Cremades, A.; Abd-Alsamad, I.; Farcet, J.; Gaulard, P.; Castellano, F.; Molinier-Frenkel, V.
The novel immunosuppressive enzyme IL4I1 is expressed by neoplastic cells of several B-cell lymphomas and by tumor-associated macrophages
Leukemia
23
952-960
2009
Homo sapiens
Manually annotated by BRENDA team
Puiffe, M.L.; Lachaise, I.; Molinier-Frenkel, V.; Castellano, F.
Antibacterial properties of the mammalian L-amino acid oxidase IL4I1
PLoS ONE
8
e54589
2013
Homo sapiens
Manually annotated by BRENDA team