Information on EC 1.4.1.4 - glutamate dehydrogenase (NADP+)

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
1.4.1.4
-
RECOMMENDED NAME
GeneOntology No.
glutamate dehydrogenase (NADP+)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH + H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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oxidative deamination
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-
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redox reaction
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-
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reduction
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reductive amination
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Alanine, aspartate and glutamate metabolism
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Arginine biosynthesis
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glutamate and glutamine metabolism
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L-glutamate biosynthesis III
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Metabolic pathways
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nitrate reduction V (assimilatory)
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nitrate reduction VI (assimilatory)
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Nitrogen metabolism
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partial TCA cycle (obligate autotrophs)
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SYSTEMATIC NAME
IUBMB Comments
L-glutamate:NADP+ oxidoreductase (deaminating)
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CAS REGISTRY NUMBER
COMMENTARY hide
9029-11-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
NADPH-GDH isozymes GDHI, GDHII, and GDHI'
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-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
strain JCM 9820
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
strain 7324
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-
Manually annotated by BRENDA team
strain NCIM 1225
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-
Manually annotated by BRENDA team
strain NCIM 1225
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-
Manually annotated by BRENDA team
strain NCIM 1211
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-
Manually annotated by BRENDA team
strain NCIM 565
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-
Manually annotated by BRENDA team
strain ATCC
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-
Manually annotated by BRENDA team
strain ATCC
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-
Manually annotated by BRENDA team
strain NCIM 553
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-
Manually annotated by BRENDA team
strain NCIM 553
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-
Manually annotated by BRENDA team
strain NCIM 656
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-
Manually annotated by BRENDA team
strain KSM-635
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Manually annotated by BRENDA team
strain KSM-635
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Manually annotated by BRENDA team
hosts human adult and human infant
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Manually annotated by BRENDA team
strain breve a and parvolorum a
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Manually annotated by BRENDA team
hosts rat and calf
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Manually annotated by BRENDA team
strain infantis a and liberorum
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Manually annotated by BRENDA team
the hosts are pig and chicken
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Manually annotated by BRENDA team
host pig
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-
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain H5-3
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Manually annotated by BRENDA team
strain H5-3
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Manually annotated by BRENDA team
strain Y7426
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Manually annotated by BRENDA team
strain Y7426
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Manually annotated by BRENDA team
strain IFO 12964
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Manually annotated by BRENDA team
strain IFO 12964
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-
Manually annotated by BRENDA team
Escherichia coli DH5-alpha
strain DH5alpha
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Manually annotated by BRENDA team
strain K12
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Manually annotated by BRENDA team
strain K2
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Manually annotated by BRENDA team
strain MRE 600
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Manually annotated by BRENDA team
strain NRC 36014
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
monokaryotic strain h1
Uniprot
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
strain F-5-2
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Manually annotated by BRENDA team
strain MK53
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Manually annotated by BRENDA team
CBS6556
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-
Manually annotated by BRENDA team
CBS6556
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-
Manually annotated by BRENDA team
strain S238N
Uniprot
Manually annotated by BRENDA team
strain S238N
Uniprot
Manually annotated by BRENDA team
strain IFO 3071
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-
Manually annotated by BRENDA team
strain IFO 3071
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-
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
strain IFO 12546
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Manually annotated by BRENDA team
strain NCDO1867
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-
Manually annotated by BRENDA team
strain CDC46
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-
Manually annotated by BRENDA team
strain X14
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-
Manually annotated by BRENDA team
strain X14
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-
Manually annotated by BRENDA team
no activity in Amanita citrina
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Manually annotated by BRENDA team
no activity in Amanita muscaria
strain MAN
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Manually annotated by BRENDA team
no activity in Amanita muscaria MAN
strain MAN
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-
Manually annotated by BRENDA team
no activity in Boletus edulis
strain Hub 0
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Manually annotated by BRENDA team
no activity in Boletus edulis Hub 0
strain Hub 0
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Manually annotated by BRENDA team
no activity in Lactarius quietus
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Manually annotated by BRENDA team
no activity in Lactarius subdulcis
strain BRI 2
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Manually annotated by BRENDA team
no activity in Lactarius subdulcis BRI 2
strain BRI 2
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Manually annotated by BRENDA team
no activity in Paxillus involutus
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Manually annotated by BRENDA team
no activity in Pisolithus tinctorius
strain 441
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Manually annotated by BRENDA team
no activity in Pisolithus tinctorius 441
strain 441
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Manually annotated by BRENDA team
no activity in Rhizopogon luteolus
strain SERRE
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Manually annotated by BRENDA team
no activity in Rhizopogon luteolus SERRE
strain SERRE
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Manually annotated by BRENDA team
no activity in Scleroderma citrinum
strain Foug A
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Manually annotated by BRENDA team
no activity in Scleroderma citrinum Foug A
strain Foug A
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Manually annotated by BRENDA team
no activity in Suillus bovinus
strain LED 1
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Manually annotated by BRENDA team
no activity in Suillus bovinus LED 1
strain LED 1
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Manually annotated by BRENDA team
no activity in Thelephora terrestris
strain CHA
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Manually annotated by BRENDA team
no activity in Thelephora terrestris CHA
strain CHA
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Manually annotated by BRENDA team
no activity in Tricholoma populinum
strain BLAE
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Manually annotated by BRENDA team
no activity in Tricholoma populinum BLAE
strain BLAE
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Manually annotated by BRENDA team
no activity in Xerocomus chrysenteron
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Manually annotated by BRENDA team
four putative GDH genes (OsGDH1-4) are present in the rice genome. The GDH sequences from rice and other species can be classified into two types (I and II). OsGDH1-3 belong to type II genes, whereas OsGDH4 belong to type I like gene
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Manually annotated by BRENDA team
strain DS12975
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Manually annotated by BRENDA team
strain NCAIM 00237
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
NADPH-GDH isozymes GDHI, GDHII, and GDHI'
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Manually annotated by BRENDA team
strain TAD1
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Manually annotated by BRENDA team
strain TAD1
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Manually annotated by BRENDA team
genes pcal_1031 and pcal_1606
UniProt
Manually annotated by BRENDA team
genes pcal_1031 and pcal_1606
UniProt
Manually annotated by BRENDA team
strain DSM 3773
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Manually annotated by BRENDA team
two NADP+-dependent glutamate dehydrogenases encoded by GDH1 and GDH3
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Manually annotated by BRENDA team
strains 975h- and 972h+
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Manually annotated by BRENDA team
Sphaerostilbe repens
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Manually annotated by BRENDA team
strain IFO 12553
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Manually annotated by BRENDA team
strain IFO 12553
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
strain PCC 6803
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Manually annotated by BRENDA team
strain KOD1
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
isolate AN1
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Manually annotated by BRENDA team
Thermophilic bacillus
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain ATCC 95640
Uniprot
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-aminopentanoate + H2O + NADP+
2-oxopentanoate + NH3 + NADPH + H+
show the reaction diagram
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activity with 2-oxovalerate amd 2-aminopentanoate is 25% and 17% compared to the activity with 2-oxoglutarate and L-glutamate
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r
2-oxoadipate + NADPH + NH3
L-2-aminoadipate + NADP+ + H2O
show the reaction diagram
2-oxobutyrate + NADPH + NH3
L-2-aminobutyrate + NADP+ + H2O
show the reaction diagram
2-oxoglutarate + NAD(P)H + NH3
L-glutamate + NAD(P)+ + H2O
show the reaction diagram
2-oxoglutarate + NADPH + NH3
L-glutamate + NADP+ + H2O
show the reaction diagram
2-oxoglutarate + NH3 + NADH
L-glutamate + H2O + NAD+
show the reaction diagram
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-
-
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r
2-oxoglutarate + NH3 + NADH + H+
L-glutamate + H2O + NAD+
show the reaction diagram
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weak activity
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-
?
2-oxoglutarate + NH3 + NADPH
L-glutamate + H2O + NADP+
show the reaction diagram
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r
2-oxoglutarate + NH3 + NADPH + H+
L-glutamate + H2O + NADP+
show the reaction diagram
2-oxoglutarate + NH4+ + NADPH
L-glutamate + NADP+
show the reaction diagram
2-oxovalerate + NADPH + NH3
L-2-aminovalerate + NADP+ + H2O
show the reaction diagram
-
15% of the activity with 2-oxoglutarate
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-
?
L-2-oxoglutarate + NADPH + NH3
glutamate + NADP+ + H2O
show the reaction diagram
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?
L-glutamate + H2O + NADP+
2-oxoglutarate + NH3 + NADPH
show the reaction diagram
L-glutamate + H2O + NADP+
2-oxoglutarate + NH3 + NADPH + H+
show the reaction diagram
L-glutamate + NAD(P)+ + H2O
2-oxoglutarate + NAD(P)H + NH3
show the reaction diagram
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r
L-glutamate + NADP+ + H2O
2-oxoglutarate + NADPH + NH3
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-oxoglutarate + NADPH + NH3
L-glutamate + NADP+ + H2O
show the reaction diagram
2-oxoglutarate + NH3 + NADPH + H+
L-glutamate + H2O + NADP+
show the reaction diagram
L-glutamate + H2O + NADP+
2-oxoglutarate + NH3 + NADPH + H+
show the reaction diagram
L-glutamate + NADP+ + H2O
2-oxoglutarate + NADPH + NH3
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD(P)H
NADH
-
the ratio of turnover-number to KM-value for NADPH is 6fold higher than the ratio for NADH
additional information
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CaCl2
10 mM, 1.3fold increase in activity
K2SO4
enhances the activity by a maximum of about 280-300% at a concentration of 150-200 mM
MgSO4
10 mM, 1.3fold increase in activity
Na3PO4
enhances the activity by a maximum of about 280-300% at a concentration of 150-200 mM
Zn2+
-
forward reaction of GDH from Aspergillus terreus is 80% inhibited by 5 mM ZnCl2
additional information
-
Ca2+ has no effects on the aminating activity of NADP(H)-GDH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,4-pyridinedicarboxylate
2-Methyleneglutarate
2-oxoglutarate
4-chloromercuribenzoate
Ag2+
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at 1 mM, 60% inhibition
AgNO3
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1 mM, 30C, complete loss of aminating activity
ATP
-
at 4 mM slight inhibitory
BaCl2
-
1 mM, 30C, 15% loss of aminating activity
Ca2+
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at 1 mM 27% inhibition
CaCl2
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1 mM, 30C, 11% loss of aminating activity
CH2ICOOH
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1 mM, 30C, 15% loss of aminating activity
dimethyl ester of isophthalic acid
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dimethyl ester of isophthalate (DMIP), but not isophthalate, inhibits Aspergillus niger growth on agar as well as in liquid culture. This is ascribed to the inability of isophthalate to enter fungal mycelia. Dimethyl ester of isophthalic acid is hydrolysed intracellularly to isophthalate. Subsequent to DMIP addition, intracellular isophthalate can be demonstrated. Addition of NH4+ to DMIP-treated Aspergillus niger mycelia results in intensive vacuolation, retraction of cytoplasm and autolysis
FeSO3(NH4)2SO4
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1 mM, 30C, 14% loss of aminating activity
fumarate
glutamate
-
competitive inhibitor of the amination reaction
Glutaric acid
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at 20 mM, 25% inhibition
glyoxylate
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at 20 mM, 30% inhibition
guanidine hydrochloride
Hexachlorophene
15% inhibition at 0.016 mM; 80% inhibition at 0.016 mM
hydroxylamine
-
competitive inhibitor with ammonia and uncompetitive inhibitor with both 2-oxoglutarate and NADPH
iodoacetamide
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at 4 mM, complete inactivation
Isophthalate
Isophthalic acid
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-
L-glutamate
-
substrate inhibition above 15 mM
L-Glutamic acid
-
at 20 mM 25% inhibition
L-homoserine
-
competitive inhibitor with respect to both ammonia and glutamine
L-tryptophan
-
at 20 mM, 15% inhibition
malate
Mg2+
-
at 1 mM, 16% activity loss
Mn2+
-
at 1 mM, 19% activity loss
N-ethylmaleimide
-
at 0.8 mM, 44% inhibition
NADP+
-
inhibits at higher 2-oxoglutarate levels
nitrogen assimilation control protein
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represses the transcription of the gene gdhA
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O2
-
inactivation
oxaloacetate
p-Chloromercuriphenyl sulfonate
-
inactivetes, can be reversed by addition of cysteine
p-hydroxymercuribenzoic acid
Pb(CH3COO)2
potassium phosphate
-
over 0.1 M at oxidative deamination
pyridoxal 5'-phosphate
pyruvate
-
at 10 mM slight inhibitory
sodium dodecylsulfate
-
at 0.7% w/v after 1 h 5% activity
succinate
-
competitive inhibitor with 2 oxoglutarate, uncompetitive with NADPH and non-competitive with ammonia
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3,5-pyrazoledicarboxylate
-
activates at lower 2-oxoglutarate concentration
3,5-pyridinedicarboxylate
-
activates at lower 2-oxoglutarate concentration
acetonitrile
-
activates
DL-2-hydroxyglutarate
-
activates at lower 2-oxoglutarate concentration
ethanol
-
activates up to 40% v/v
Glutarate
-
activates at lower 2-oxoglutarate concentration
IDP
-
at 1 mM five-fold Michaelis Menten constant of 2-oxoglutarate
Itaconate
-
activates even in the presence of 10 mM 2-oxoglutarate
K2HPO4
-
at 0.15 M three to fourfold stimulation of activity
K2SO4
-
280-300% activity at 150-200 mM
K3PO4
-
less effective than K2SO4 and Na3PO4
methanol
-
activates
MgCl2
-
at 5 mM, 104% activity, oxidative deamination
MnCl2
-
at 5 mM, 250% activity, oxidative deamination
Na2SO4
-
less effective than K2SO4 and Na3PO4
Na3PO4
-
280-300% activity at 150-200 mM
oxalylglycine
-
activates at lower 2-oxoglutarate concentration
potassium phosphate
-
up to 0.1 M oxidative deamination
UTP
-
activates up to 70%
Zn2+
-
activates up to 0.1 mM
additional information
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exhibits 2.5-fold-higher GDH activity than cells are grown in milk, instead of glucose
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.018 - 606
2-oxoglutarate
0.22 - 1349
L-glutamate
3.629
NAD+
-
pH 9.0, 50C
0.36 - 0.368
NADH
0.0064 - 19.06
NADP+
0.003 - 59.7
NADPH
0.00056 - 83
NH3
0.29 - 416
NH4+
2.5 - 119
NH4Cl
additional information
additional information