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Information on EC 1.2.4.1 - pyruvate dehydrogenase (acetyl-transferring) and Organism(s) Arabidopsis thaliana

for references in articles please use BRENDA:EC1.2.4.1
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IUBMB Comments
Contains thiamine diphosphate. It is a component (in multiple copies) of the multienzyme pyruvate dehydrogenase complex, EC 1.2.1.104, in which it is bound to a core of molecules of EC 2.3.1.12, dihydrolipoyllysine-residue acetyltransferase, which also binds multiple copies of EC 1.8.1.4, dihydrolipoyl dehydrogenase. It does not act on free lipoamide or lipoyllysine, but only on the lipoyllysine residue in EC 2.3.1.12.
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Arabidopsis thaliana
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
pdh, pdha1, mitochondrial pyruvate dehydrogenase, e1alpha, pyruvate dehydrogenase multienzyme complex, pyruvate dehydrogenase e1, pdhc-e1, mtpdc, pdhc e1, pyruvate dehydrogenase e1 component, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dehydrogenase, pyruvate
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E1alpha
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component of the pyruvate dehydrogenase multienzyme complex PDH
MtPDC
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PDH
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pyruvate decarboxylase
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pyruvate dehydrogenase
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pyruvate dehydrogenase complex
pyruvate:[dihydrolipoyllysine-residue acetyltransferase]-lipoyllysine 2-oxidoreductase (decarboxylating, acceptor-acetylating)
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pyruvic acid dehydrogenase
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pyruvic dehydrogenase
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VEG220
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Vegetative protein 220
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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SYSTEMATIC NAME
IUBMB Comments
pyruvate:[dihydrolipoyllysine-residue acetyltransferase]-lipoyllysine 2-oxidoreductase (decarboxylating, acceptor-acetylating)
Contains thiamine diphosphate. It is a component (in multiple copies) of the multienzyme pyruvate dehydrogenase complex, EC 1.2.1.104, in which it is bound to a core of molecules of EC 2.3.1.12, dihydrolipoyllysine-residue acetyltransferase, which also binds multiple copies of EC 1.8.1.4, dihydrolipoyl dehydrogenase. It does not act on free lipoamide or lipoyllysine, but only on the lipoyllysine residue in EC 2.3.1.12.
CAS REGISTRY NUMBER
COMMENTARY hide
9014-20-4
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
pyruvate + CoA + NAD+
acetyl-CoA + CO2 + NADH
show the reaction diagram
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?
pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine
[dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2
show the reaction diagram
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?
additional information
?
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conjugates of indole-3-acetic acid with amino acids act as hormones and e.g. inhibit root elongation, the sensitivity of the mutant iar4-1 plant against these conjugates and plant hormones is reduced compared to the wild-type, overview
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
pyruvate + CoA + NAD+
acetyl-CoA + CO2 + NADH
show the reaction diagram
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?
pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine
[dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2
show the reaction diagram
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?
additional information
?
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conjugates of indole-3-acetic acid with amino acids act as hormones and e.g. inhibit root elongation, the sensitivity of the mutant iar4-1 plant against these conjugates and plant hormones is reduced compared to the wild-type, overview
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
thiamine diphosphate
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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effects of several plant hormones and auxin derivatives
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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effects of several plant hormones and auxin derivatives
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
mitochondrial PDC E1 contributes to polar auxin transport during organ development. MAB1 encodes a mitochondrial PDC E1beta subunit that can form both a homodimer and a heterodimer with alpha-subunit IAR4. The MAB1 mutation impairs MAB1 homodimerization, reduces the abundance of IAR4 and IAR4L, weakens PDC enzymatic activity, and diminishes mitochondrial respiration. Mutation leads to significant changes in metabolites including amino acids, and an accumulation of Ala. In MAB1 mutants and seedlings where the TCA cycle is pharmacologically blocked, reduced abundance of the PIN-FORMED (PIN) auxin efflux carriers is found
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ODPA1_ARATH
389
0
43059
Swiss-Prot
Chloroplast (Reliability: 5)
ODPA2_ARATH
393
0
43359
Swiss-Prot
Mitochondrion (Reliability: 5)
ODPA3_ARATH
428
0
47174
Swiss-Prot
Chloroplast (Reliability: 1)
ODPB3_ARATH
406
0
44015
Swiss-Prot
Chloroplast (Reliability: 1)
ODPB1_ARATH
363
0
39176
Swiss-Prot
Mitochondrion (Reliability: 1)
ODPB2_ARATH
406
0
44245
Swiss-Prot
Chloroplast (Reliability: 1)
A0A178WLI5_ARATH
307
0
33769
TrEMBL
other Location (Reliability: 1)
A0A178W8A7_ARATH
428
0
47174
TrEMBL
Chloroplast (Reliability: 1)
A0A178WAC1_ARATH
393
0
43393
TrEMBL
Mitochondrion (Reliability: 5)
A0A7G2DT04_ARATH
388
0
42898
TrEMBL
Mitochondrion (Reliability: 5)
A0A654GAS7_ARATH
363
0
39116
TrEMBL
Mitochondrion (Reliability: 1)
A0A1P8AQS2_ARATH
378
0
41896
TrEMBL
Chloroplast (Reliability: 5)
A0A5S9RZM7_ARATH
428
0
47165
TrEMBL
Chloroplast (Reliability: 1)
A0A5S9VTJ7_ARATH
393
0
43359
TrEMBL
Mitochondrion (Reliability: 5)
A0A178W125_ARATH
406
0
44015
TrEMBL
Chloroplast (Reliability: 1)
A0A178WIN0_ARATH
406
0
44259
TrEMBL
Chloroplast (Reliability: 1)
A0A7G2EFE5_ARATH
449
0
48615
TrEMBL
Chloroplast (Reliability: 1)
A0A7G2DRX4_ARATH
835
0
91330
TrEMBL
other Location (Reliability: 5)
A0A178UM82_ARATH
363
0
39176
TrEMBL
Mitochondrion (Reliability: 1)
A0A178WNY2_ARATH
389
0
43059
TrEMBL
Chloroplast (Reliability: 5)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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construction of the iar4-1 mutant, defective in gene iar4 encoding the enzyme E1, by ethylmethane sulfonate, the mutant is less sensitive to a synthetic auxin and low concentrations of an ethylene precursor, but responds to free indole-3-acetic acid and other hormones in a way similar to the wild-type, the Krebs cycle and glycolysis are unaffected
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene iar4, wild-type sequencing and chromosomal mapping to a 45 kb region 35 cM from the top of chromosome 1 between the markers F3I6.8 and F3I6.17, DNA sequence determination of iar4-1 mutant
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
LeClere, S.; Rampey, R.A.; Bartel, B.
IAR4, a gene required for auxin conjugate sensitivity in Arabidopsis, encodes a pyruvate dehydrogenase E1alpha homolog
Plant Physiol.
135
989-999
2004
Arabidopsis thaliana
Manually annotated by BRENDA team
Broz, A.K.; Tovar-Mendez, A.; Mooney, B.P.; Johnston, M.L.; Miernyk, J.A.; Randall, D.D.
A novel regulatory mechanism based upon a dynamic core structure for the mitochondrial pyruvate dehydrogenase complex?
Mitochondrion
19
144-153
2014
Arabidopsis thaliana
Manually annotated by BRENDA team
Ohbayashi, I.; Huang, S.; Fukaki, H.; Song, X.; Sun, S.; Morita, M.T.; Tasaka, M.; Millar, A.H.; Furutani, M.
Mitochondrial pyruvate dehydrogenase contributes to auxin-regulated organ development
Plant Physiol.
180
896-909
2019
Arabidopsis thaliana (Q8H1Y0 and Q38799)
Manually annotated by BRENDA team