Information on EC 1.2.3.1 - aldehyde oxidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
1.2.3.1
-
RECOMMENDED NAME
GeneOntology No.
aldehyde oxidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
an aldehyde + H2O + O2 = a carboxylate + H2O2
show the reaction diagram
-
-
-
-
an aldehyde + H2O + O2 = a carboxylate + H2O2
show the reaction diagram
concerted mechanism
synthetic construct
-
an aldehyde + H2O + O2 = a carboxylate + H2O2
show the reaction diagram
base catalyzed mechanism. Residue E1265 acts as an active site base that abstracts a proton from the Mo-OH group, which in turn undertakes a nucleophilic attack on the substrate benzaldehyde. After hydride transfer to the Mo= S group, the initial intermediate breaks down, with the transient formation of a paramagnetic MoV species, followed by displacement of product by a water molecule to return to the starting LMoVIOS(OH) state. The roles of residues Met884 and Val806 are stabilization of substrate binding
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Drug metabolism - cytochrome P450
-
-
Metabolic pathways
-
-
Microbial metabolism in diverse environments
-
-
Nicotinate and nicotinamide metabolism
-
-
Retinol metabolism
-
-
Tryptophan metabolism
-
-
Tyrosine metabolism
-
-
Valine, leucine and isoleucine degradation
-
-
Vitamin B6 metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
aldehyde:oxygen oxidoreductase
Contains molybdenum, [2Fe-2S] centres and FAD. The enzyme from liver exhibits a broad substrate specificity, and is involved in the metabolism of xenobiotics, including the oxidation of N-heterocycles and aldehydes and the reduction of N-oxides, nitrosamines, hydroxamic acids, azo dyes, nitropolycyclic aromatic hydrocarbons, and sulfoxides [4,6]. The enzyme is also responsible for the oxidation of retinal, an activity that was initially attributed to a distinct enzyme (EC 1.2.3.11, retinal oxidase) [5,7].
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
AHO2
-
-
AHO2
Mus musculus CD-1, Mus musculus DBA/2
-
-
-
aldehyde oxidase 1
P48034
-
aldehyde oxidase 1
-
-
aldehyde oxidase 1
Q2QB50
-
aldehyde oxidase 1
-
-
aldehyde oxidase 1
Q06278
-
aldehyde oxidase 1
-
-
aldehyde oxidase 1
-
-
aldehyde oxidase 1
Q6V956
-
aldehyde oxidase 1
-
-
aldehyde oxidase 1
P80456
-
aldehyde oxidase 1
B0LAZ9
isoform
aldehyde oxidase 1
-
-
aldehyde oxidase 1
Q5QE80
-
aldehyde oxidase 2
Q2QB48
-
aldehyde oxidase 2
Q2QB49
-
aldehyde oxidase 2
B0LB00
isoform
aldehyde oxidase 2
Q5QE79
-
aldehyde oxidase 3
Q2QB47
-
aldehyde oxidase 3
-
-
aldehyde oxidase 3
Q5SGK3
-
aldehyde oxidase 3
B0LB01
isoform
aldehyde oxidase 3
Q5QE78
-
aldehyde oxidase 3-like 1
-
-
aldehyde oxidase 4
-
-
aldehyde-oxygen oxidoreductase
-
-
aldehyde:oxygen oxidoreductase
-
-
antennae-specific aldehyde oxidase
S5FPI8
-
AO
-
-
AO-alpha
-
isoform
AO-beta
-
isoform
AO-delta
-
isoform
AO-gamma
-
isoform
AO-kappa
-
isoform
AO1
B0LAZ9
isoform
AO2
B0LB00
isoform
AO3
B0LB01
isoform
AOH
Q2QB49
-
AOH1
-
-
AOH1
Q8VJ15
-
AOH1
Mus musculus CD-1, Mus musculus DBA/2
-
-
-
AOH1
Q5QE80
-
AOH2
Q5QE79
-
AOH3
Q5QE78
-
AOX
-
-
AOX1
A8TUB4
-
AOX1
Bombyx mori Nistari
A8TUB4
-
-
AOX1
P48034
-
AOX1
-
-
AOX1
Q2QB50
-
AOX1
Q06278
-
AOX1
Q5FB27
-
AOX1
-
-
AOX1
O54754
-
AOX1
Mus musculus CD-1, Mus musculus DBA/2
-
-
-
AOX1
P80456
gene name
AOX1
Q9Z0U5
-
AOX1
Q6GMC5
-
AOX1
O23887
-
AOX2
A8TUC0
-
AOX2
Bombyx mori Nistari
A8TUC0
-
-
AOX2
O23888
-
AOX3
G3X982
-
AtraAOX2
S5FPI8
-
formate oxidase
-
-
mAOX3
-
-
mAOX3
G3X982
-
mouse liver aldehyde oxidase 3
G3X982
-
quinoline oxidase
-
-
-
-
Retinal oxidase
-
-
-
-
Retinal oxidase
-
-
retinene oxidase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9029-07-6
-
9033-52-7
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
navel orangeworm
UniProt
Manually annotated by BRENDA team
Ler wild type
-
-
Manually annotated by BRENDA team
Nistari strain
UniProt
Manually annotated by BRENDA team
Bombyx mori Nistari
Nistari strain
UniProt
Manually annotated by BRENDA team
isoform aldehyde oxidase 2
SwissProt
Manually annotated by BRENDA team
isoform aldehyde oxidase 3
SwissProt
Manually annotated by BRENDA team
Dunkin-Hartley guinea pigs
-
-
Manually annotated by BRENDA team
house mosquito, organophosphorus insecticide-resistant strain PelRR, aldehyde oxidase may play a role in insecticide resistance
SwissProt
Manually annotated by BRENDA team
strain DSM 12254, aminolytic strain performing thiosulfate dismutation
-
-
Manually annotated by BRENDA team
lacks FAD
-
-
Manually annotated by BRENDA team
isoform aldehyde oxidase 1
SwissProt
Manually annotated by BRENDA team
isoform aldehyde oxidase 2
SwissProt
Manually annotated by BRENDA team
Japanese children
-
-
Manually annotated by BRENDA team
fragment
SwissProt
Manually annotated by BRENDA team
fragment of sequence
SwissProt
Manually annotated by BRENDA team
; CD-1 mouse; DBA/2 mouse
-
-
Manually annotated by BRENDA team
DBA/2, CBA/2 C57Bl/6J and CD1 mice
-
-
Manually annotated by BRENDA team
DBA/2, CBA/2 C57Bl/6J and CD1 mice
UniProt
Manually annotated by BRENDA team
enzyme isoform AOX1 gene and its homologue AOH1 are inducible by 2,3,7,8-tetrachlorodibenzo-p-dioxin. Transcriptional induction through aryl hydrocarbon receptor pathway
-
-
Manually annotated by BRENDA team
strain CD-1
-
-
Manually annotated by BRENDA team
Mus musculus CD-1
CD-1 mouse
-
-
Manually annotated by BRENDA team
Mus musculus CD-1
strain CD-1
-
-
Manually annotated by BRENDA team
Mus musculus DBA/2
DBA/2 mouse
-
-
Manually annotated by BRENDA team
no activity in Aspergillus nidulans
-
-
-
Manually annotated by BRENDA team
New Zealand rabbit
UniProt
Manually annotated by BRENDA team
Hass avocado
-
-
Manually annotated by BRENDA team
cultivar Little Marvel
-
-
Manually annotated by BRENDA team
cultivar Little Marvel
UniProt
Manually annotated by BRENDA team
KY 4690
-
-
Manually annotated by BRENDA team
strain AIU 362
-
-
Manually annotated by BRENDA team
strain AIU 362
-
-
Manually annotated by BRENDA team
Pseudomonas sp. KY 4690
KY 4690
-
-
Manually annotated by BRENDA team
Pseudomonas stutzeri 12695
12695
-
-
Manually annotated by BRENDA team
Donryu strain rats
-
-
Manually annotated by BRENDA team
male sprague-dawley rats
-
-
Manually annotated by BRENDA team
marked variations in enzyme activity of different strains
-
-
Manually annotated by BRENDA team
Sea:SD rats
-
-
Manually annotated by BRENDA team
study on ten different strains
UniProt
Manually annotated by BRENDA team
Wistar rats and Donryu rats
-
-
Manually annotated by BRENDA team
Wistar rats and Donryu rats
UniProt
Manually annotated by BRENDA team
Wistar strain rats
-
-
Manually annotated by BRENDA team
Rattus norvegicus SpragueDawley
-
-
-
Manually annotated by BRENDA team
experimental thyroid disorders
-
-
Manually annotated by BRENDA team
wild-type tomato and the Moco sulfurase mutant flacca. Upregulation of enzyme mRNA by abscisic acid and water-stress in leaves and roots and of enzyme synthesis and activity by drought
-
-
Manually annotated by BRENDA team
strain ATCC10970, enzyme belongs to xanthine oxidase family
-
-
Manually annotated by BRENDA team
Streptomyces rimosus ATCC10970
strain ATCC10970, enzyme belongs to xanthine oxidase family
-
-
Manually annotated by BRENDA team
synthetic construct
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
-
aldehyde oxidase catalyzes the final step in the synthesis of abscisic acid and possibly of indole-3-acetic acid
physiological function
-
AOX3 is an enzyme of well known importance in drug metabolism and therefore of increasing importance in recent drug design programs
physiological function
S5FPI8
AtraAOX2 could be involved in detoxifying plant-derived toxic aldehydes and aldehyde-containing pesticides
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine + H2O + O2
?
show the reaction diagram
-
i.e. (S)-RS-8359
-
-
?
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine + H2O + O2
?
show the reaction diagram
-
i.e. (S)-RS-8359
-
-
?
(+)-biotin (+)-sulfoxide methyl ester + acetaldehyde + H2O
?
show the reaction diagram
-
under anaerobic conditions
-
-
?
(+/-)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine + H2O + O2
?
show the reaction diagram
-
i.e. RS-8359
-
-
?
(1S)-camphor oxime + H2O + 2-hydroxypyrimidine
(1S)-camphor + (1S)-camphor imine + NH3
show the reaction diagram
-
other electron acceptors are N-methylnicotinamide, butyraldehyde and benzaldehyde
the corresponding ketimine is an intermediate
?
(2E)-hex-2-enal + H2O + O2
(2E)-hex-2-enoic acid + H2O2
show the reaction diagram
S5FPI8
-
-
-
?
(2E)-non-2-enal + H2O + O2
(2E)-non-2-enoic acid + H2O2
show the reaction diagram
S5FPI8
-
-
-
?
(S)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine + H2O + O2
? + H2O2
show the reaction diagram
-
i.e. S-8359
-
-
?
(S)-RS-8359
?
show the reaction diagram
-
-
-
-
?
(S)-RS-8359
?
show the reaction diagram
Q5FB27
enzyme shows a biphasic pattern in EadieHofstee plots of the (S)-enantiospecific 2-oxidation activity of RS-8359
-
-
?
(S)-RS-8359
?
show the reaction diagram
-
enzyme shows a monophasic pattern in Eadie-Hofstee plots of the (S)-enantiospecific 2-oxidation activity of RS-8359
-
-
?
(S)-RS-8359 + H2O + O2
2-keto-(S)-RS-8359 + H2O2
show the reaction diagram
Q9Z0U5
i.e. 4-([(7S)-7-hydroxy-6,7-dihydro-5H-cyclopenta[d]pyrimidin-4-yl]amino)benzonitrile, reversible inhibitor of monoamine oxidase A. Remarkable strain differences in activity on substate, correlating in part with expressed levels of protein
-
-
?
(S)-RS-8359 + H2O + O2
2-keto-(S)-RS-8359 + H2O2
show the reaction diagram
Q5QE78, Q5QE79, Q5QE80, Q9Z0U5
Donryu rats show a dimorphic pattern for the 2-oxidation activity of RS-8359
-
-
?
(Z)-11-hexadecenal + H2O + O2
(Z)-11-hexadecenoic acid + H2O2
show the reaction diagram
-
-
-
-
?
(Z)-7-dodecenal + H2O + O2
(Z)-7-dodecenoic acid + H2O2
show the reaction diagram
-
-
-
-
?
(Z)-7-tetradecenal + H2O + O2
(Z)-7-tetradecenoic acid + H2O2
show the reaction diagram
-
-
-
-
?
(Z)-9-hexadecenal + H2O + O2
(Z)-9-hexadecenoic acid + H2O2
show the reaction diagram
-
-
-
-
?
(Z)-9-tetradecenal + H2O + O2
(Z)-9-tetradecenoic acid + H2O2
show the reaction diagram
-
-
-
-
?
1-methoxy-2-naphthaldehyde + H2O + O2
1-methoxy-2-naphthoic acid + H2O2
show the reaction diagram
-
-
-
-
?
1-methyl-acetylpyridine + H2O + ferricyanide
?
show the reaction diagram
-
-
-
-
?
1-naphthaldehyde + H2O + O2
1-naphthoic acid + H2O2
show the reaction diagram
-
-
-
-
?
1-nitropyrene + H2O + O2
?
show the reaction diagram
P80456
-
-
-
?
13-cis-retinal + O2 + H2O
13-cis-retinoate + H2O2
show the reaction diagram
-
-
-
?
2,3-diazanaphthalene + O2
1-phthalazinone
show the reaction diagram
-
-
-
-
?
2-ethylbutylaldehyde + H2O + O2
2-ethylbutanoate + H2O2
show the reaction diagram
-
170% of activity with formaldehyde
-
-
?
2-ethylbutylaldehyde + H2O + O2
2-ethylbutanoate + H2O2
show the reaction diagram
-
350% of activity with formaldehyde
-
-
?
2-ethylbutylaldehyde + H2O + O2
2-ethylbutanoate + H2O2
show the reaction diagram
-
51% of activity with formaldehyde
-
-
?
2-hydroxybenzaldehyde + H2O + O2
2-hydroxybenzoate + H2O2
show the reaction diagram
-
-
-
-
?
2-hydroxypyrimidine + ferricyanide
?
show the reaction diagram
Mus musculus, Mus musculus CD-1, Mus musculus DBA/2
-
-
-
-
?
2-hydroxypyrimidine + H2O + ferricyanide + O2
?
show the reaction diagram
-
-
-
-
?
2-hydroxypyrimidine + H2O + O2
uracil + H2O2
show the reaction diagram
-
-
-
-
?
2-hydroxypyrimidine + H2O + O2
uracil + H2O2
show the reaction diagram
-
-
-
-
?
2-hydroxypyrimidine + H2O + O2
uracil + H2O2
show the reaction diagram
-
-
-
-
?
2-hydroxypyrimidine + H2O + O2
uracil + H2O2
show the reaction diagram
-
ferricyanide as electron acceptor under aerobic conditions
-
-
?
2-hydroxypyrimidine + H2O + O2
uracil + H2O2
show the reaction diagram
-
diphenylsulfoxide as electron acceptor under anaerobic conditions
-
-
-
2-hydroxypyrimidine + H2O + O2
uracil + H2O2
show the reaction diagram
-
diphenylsulfoxide as electron acceptor under anaerobic conditions
-
-
?
2-hydroxypyrimidine + O2 + H2O
? + H2O2
show the reaction diagram
-
-
-
?
2-hydroxypyrimidine + O2 + H2O
?
show the reaction diagram
-
-
-
-
?
2-methoxybenzaldehyde + H2O + O2
2-methoxybenzoate + H2O2
show the reaction diagram
-
-
-
-
?
2-methylbenzaldehyde + H2O + O2
2-methylbenzoate + H2O2
show the reaction diagram
Pseudomonas stutzeri, Pseudomonas stutzeri 12695
-
7% of activity with n-hexylaldehyde
-
-
?
2-naphthaldehyde + H2O + O2
2-naphthoic acid + H2O2
show the reaction diagram
-
-
-
-
?
2-pyrimidone + H2O + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
3 brimonidine + 4 H2O + 4 O2
2-oxobrimonidine + 3-oxobrimonidine + 2,3-dioxobrimonidine + 4 H2O2
show the reaction diagram
-
-
-
-
?
3,4-dihydroxybenzaldehyde + H2O + O2
3,4-dihydroxybenzoate + H2O2
show the reaction diagram
-
38.2% of the rate with benzaldehyde
-
-
?
3,4-dimethoxybenzaldehyde + H2O + O2
3,4-dimethoxybenzoate + H2O2
show the reaction diagram
-
-
-
-
?
3-(5H-dibenzo[a,d]cyclohepten-5-ylidene)-N,N-dimethyl-1-propanamine N-oxide + electron donor
3-(5H-dibenzo[a,d]cyclohepten-5-ylidene)-N,N-dimethyl-1-propanamine + an electron acceptor
show the reaction diagram
-
electron donors are 2-hydroxypyrimidine, N1-methylnicotinamide, benzaldehyde or butyraldehyde under anaerobic conditions
-
-
?
3-aminocarbonyl-1-methylpyridinium chloride + H2O + O2
?
show the reaction diagram
-
-
-
-
?
3-hydroxybenzaldehyde + H2O + O2
3-hydroxybenzoate + H2O2
show the reaction diagram
-
-
-
-
?
3-methoxy-2-naphthaldehyde + H2O + O2
3-methoxy-2-naphthoic acid + H2O2
show the reaction diagram
-
-
-
-
?
3-methoxy-4-hydroxybenzaldehyde + H2O + O2
3-methoxy-4-hydroxybenzoate + H2O2
show the reaction diagram
-
-
-
-
?
3-methoxybenzaldehyde + H2O + O2
3-methoxybenzoate + H2O2
show the reaction diagram
-
-
-
-
?
3-methylbenzaldehyde + H2O + O2
3-methylbenzoate + H2O2
show the reaction diagram
Pseudomonas stutzeri, Pseudomonas stutzeri 12695
-
42% of activity with n-hexylaldehyde
-
-
?
4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine + H2O + O2
? + H2O2
show the reaction diagram
-
i.e. RS-8359, 2-oxidation of the S-enantiomer
-
-
?
4-(dimethylamino)-cinnamaldehyde + H2O + O2
?
show the reaction diagram
-
-
-
-
?
4-(dimethylamino)cinnamaldehyde + H2O + O2
?
show the reaction diagram
-
-
-
-
?
4-(dimethylamino)cinnamaldehyde + nitrite + H2O + O2
? + nitric oxide
show the reaction diagram
-
in the presence of typical aldehyde substrates like 4-(dimethylamino)cinnamaldehyde or NADH, aldehyde oxidase reduces nitrite to nitric oxide
-
-
?
4-(dimethylamino)cinnamaldehyde + O2 + H2O
4-(dimethylamine)cinnamate + H2O2
show the reaction diagram
-
-
-
?
4-(dimethylamino)cinnamaldehyde + O2 + H2O
4-(dimethylamine)cinnamate + H2O2
show the reaction diagram
-
typical substrate spectrum of aldehyde oxidase
-
?
4-hydroxybenzaldehyde + H2O + O2
4-hydroxybenzoate + H2O2
show the reaction diagram
-
-
-
-
?
4-hydroxypyrazolo(3,4-d)pyrimidine + O2 + H2O
4,6-dihydroxypyrazolo(3,4-d)pyrimidine + H2O2
show the reaction diagram
-
12-13% of the activity compared to purine, i.e. allopurinol
-
?
4-hydroxypyrimidine + H2O + O2
?
show the reaction diagram
-
ferricyanide as electron acceptor under aerobic conditions
-
-
?
4-methoxybenzaldehyde + H2O + O2
4-methoxybenzoate + H2O2
show the reaction diagram
-
-
-
-
?
4-methylbenzaldehyde + H2O + O2
4-methylbenzoate + H2O2
show the reaction diagram
Pseudomonas stutzeri, Pseudomonas stutzeri 12695
-
40% of activity with n-hexylaldehyde
-
-
?
4-nitrobenzaldehyde + H2O + O2
4-nitrobenzoic acid + H2O2
show the reaction diagram
-
-
-
-
?
5-fluoropyrimidine + H2O + O2
5-fluorouracil + H2O2
show the reaction diagram
-
-
-
-
?
6-benzylguanine + H2O + O2
?
show the reaction diagram
-
-
-
-
?
6-chloroquinazolin-4(3H)-one + H2O + O2
6-chloroquinazoline-2,4(1H,3H)-dione + H2O2
show the reaction diagram
-
-
-
-
?
6-deoxypenciclovir
penciclovir
show the reaction diagram
-
is catalyzed by AOX1
-
-
?
6-deoxypenciclovir + H2O + O2
?
show the reaction diagram
-
-
-
-
?
6-dimethyloamino-2-naphthaldehyde + H2O + O2
6-dimethylamino-2-naphthoic acid + H2O2
show the reaction diagram
-
-
-
-
?
6-ethyl-5H-dibenz(c,e)azepine + H2O + O2
?
show the reaction diagram
-
2,6-dichlorophenol-indophenol as electron acceptor
-
-
?
6-mercaptopurine + H2O + O2
?
show the reaction diagram
-
-
-
-
?
6-methoxy-2-naphthaldehyde + H2O + O2
6-methoxy-2-naphthoic acid + H2O2
show the reaction diagram
-
-
-
-
?
6-methoxyquinazolin-4(3H)-one + H2O + O2
6-methoxyquinazoline-2,4(1H,3H)-dione + H2O2
show the reaction diagram
-
-
-
-
?
6-methylpurine + H2O + chromate
?
show the reaction diagram
-
-
-
-
?
6-methylquinazolin-4(3H)-one + H2O + O2
6-methylquinazoline-2,4(1H,3H)-dione + H2O2
show the reaction diagram
-
-
-
-
?
6-methylthiopurine + H2O + O2
6-methylthio-8-hydroxypurine + H2O2
show the reaction diagram
-
2,6-dichlorophenol indophenol can also act as electron acceptor
-
-
?
6-thioxanthine + H2O + O2
6-thiouric acid + H2O2
show the reaction diagram
-
-
-
-
?
7-methoxy-1-naphthaldehyde + H2O + O2
7-methoxy-2-naphthoic acid + H2O2
show the reaction diagram
-
-
-
-
?
9-cis-retinal + O2 + H2O
9-cis-retinoate + H2O2
show the reaction diagram
-
-
-
?
abscisic aldehyde + H2O + O2
abscisic acid + H2O2
show the reaction diagram
B0LAZ9, B0LB00, B0LB01
-
-
-
?
abscisic aldehyde + H2O + O2
abscisic acid + H2O2
show the reaction diagram
-
four aldehyde oxidases are known that have varying affinities to abscisic aldehyde. AOdelta, endcoded by AAO3 specifically catalyses this step in rosette leaves
-
-
?
abscisic aldehyde + O2 + H2O
abscisic acid + H2O2
show the reaction diagram
-
-
-
-
?
acetaldehyde + 2 ferricyanide + H2O
acetate + 2 ferrocyanide + 2 H+
show the reaction diagram
Mus musculus, Mus musculus CD-1, Mus musculus DBA/2
-
-
-
-
?
acetaldehyde + H2O + O2
acetic acid + H2O2
show the reaction diagram
-
-
-
-
?
acetaldehyde + H2O + O2
acetic acid + H2O2
show the reaction diagram
-
-
-
-
?
acetaldehyde + H2O + O2
acetic acid + H2O2
show the reaction diagram
-
-
-
-
?
acetaldehyde + H2O + O2
acetic acid + H2O2
show the reaction diagram
-
-
-
-
?
acetaldehyde + H2O + O2
acetic acid + H2O2
show the reaction diagram
-
-
-
-
?
acetaldehyde + H2O + O2
acetic acid + H2O2
show the reaction diagram
-
-
-
-
?
acetaldehyde + H2O + O2
acetic acid + H2O2
show the reaction diagram
S5FPI8
-
-
-
?
acetaldehyde + H2O + O2
acetic acid + H2O2
show the reaction diagram
-
ferricyanide as electron acceptor under aerobic conditions
-
-
?
acetaldehyde + H2O + O2
acetic acid + H2O2
show the reaction diagram
-
2,6-dichlorophenol-indophenol as electron acceptor
-
-
?
acetaldehyde + H2O + O2
acetic acid + H2O2
show the reaction diagram
-
2,6-dichlorophenol-indophenol as electron acceptor
-
-
?
acetaldehyde + H2O + O2
acetic acid + H2O2
show the reaction diagram
-
2,6-dichlorophenol-indophenol as electron acceptor
-
-
?
acetaldehyde + H2O + O2
acetic acid + H2O2
show the reaction diagram
-
2,6-dichlorophenol-indophenol as electron acceptor
-
-
?
acetaldehyde + H2O + O2
acetic acid + H2O2
show the reaction diagram
-
-
-
-
?
acetaldehyde + H2O + O2
acetic acid + H2O2
show the reaction diagram
Streptomyces rimosus ATCC10970
-
-
-
-
?
acetaldehyde + H2O + O2
acetic acid + H2O2
show the reaction diagram
Desulfovibrio aminophilus DSM12254
-
-
-
-
?
acetaldehyde + H2O + O2
acetate + H2O2
show the reaction diagram
Q9NCL9
-
-
-
?
acetaldehyde + H2O + O2
acetate + H2O2
show the reaction diagram
-
170% of activity with formaldehyde
-
-
?
acetaldehyde + H2O + O2
acetate + H2O2
show the reaction diagram
-
35% of activity with formaldehyde
-
-
?
acetaldehyde + H2O + O2
acetate + H2O2
show the reaction diagram
-
350% of activity with formaldehyde
-
-
?
acetaldehyde + H2O + O2
acetate + H2O2
show the reaction diagram
-
51% of activity with n-hexylaldehyde
-
-
?
acetaldehyde + H2O + O2
acetate + H2O2
show the reaction diagram
O54754, Q5SGK3, Q6V956, Q8VJ15
is a poor substrate of AOH1
-
-
?
acetaldehyde + H2O + O2
acetate + H2O2
show the reaction diagram
O54754, Q5SGK3, Q6V956, Q8VJ15
is a poor substrate of AOX1
-
-
?
acetaldehyde + H2O + O2
acetate + H2O2
show the reaction diagram
Pseudomonas stutzeri 12695
-
51% of activity with n-hexylaldehyde
-
-
?
acetaldehyde + O2 + H2O
acetate + H2O2
show the reaction diagram
Pseudomonas sp., Pseudomonas sp. KY 469, Pseudomonas sp. KY 4690
-
-
-
-
?
acetamiprid + H2O + O2
? + H2O2
show the reaction diagram
-
i.e. (1E)-N-[(6-chloropyridin-3-yl)methyl]-N'-cyano-N-methylethanimidamide, enzyme system coupled with Drosophila nicotinic acetylcholine receptor, little inactivation of neonicotinoid insecticide substrate
-
-
?
acetophenone oxime + H2O + 2-hydroxypyrimidine
acetophenone + NH3
show the reaction diagram
-
-
-
-
?
acetophenone oxime + H2O + 2-hydroxypyrimidine
acetophenone + NH3
show the reaction diagram
-
other electron acceptors are N-methylnicotinamide, butyraldehyde and benzaldehyde
the corresponding ketimine is an intermediate
?
acrolein + H2O + O2
acrylic acid + H2O2
show the reaction diagram
-
130% of activity with formaldehyde
-
-
?
acrolein + H2O + O2
acrylic acid + H2O2
show the reaction diagram
-
140% of activity with formaldehyde
-
-
?
acrolein + H2O + O2
acrylic acid + H2O2
show the reaction diagram
-
8% of activity with formaldehyde
-
-
?
acrolein + H2O + O2
?
show the reaction diagram
S5FPI8
results indicate that AtraAOX2 also functions as a xenobiotic-degrading enzyme
-
-
?
all-trans retinaldehyde + H2O + O2
all-trans retinoic acid + H2O2
show the reaction diagram
P80456
-
-
-
?
all-trans retinaldehyde + H2O + O2
all-trans retinoic acid + H2O2
show the reaction diagram
O54754, Q5SGK3, Q6V956, Q8VJ15
-
-
-
?
all-trans retinaldehyde + H2O + O2
all-trans retinoic acid + H2O2
show the reaction diagram
O54754, Q5SGK3, Q6V956, Q8VJ15
AOH1 from the liver of CD1 mice is capable of oxidizing all-trans retinaldehyde
-
-
?
all-trans retinaldehyde + H2O + O2
all-trans retinoic acid + H2O2
show the reaction diagram
O54754, Q5SGK3, Q6V956, Q8VJ15
AOH2 from the mouse Harderian gland and AOH3 from the mouse Bowman's gland are all capable of oxidizing all-trans retinaldehyde with equal efficiency
-
-
?
all-trans-retinal + O2 + H2O
all-trans-retinoate + H2O2
show the reaction diagram
-
-
-
-
?
all-trans-retinal + O2 + H2O
all-trans-retinoate + H2O2
show the reaction diagram
-
-
-
?
all-trans-retinal + O2 + H2O
all-trans-retinoate + H2O2
show the reaction diagram
-
-
-
?
all-trans-retinal + O2 + H2O
all-trans-retinoate + H2O2
show the reaction diagram
-
-
-
?
anthracene-9-carboxaldehyde + H2O + O2
anthracene-9-carboxylate + H2O2
show the reaction diagram
-
-
-
-
?
benzaldehyde + 2 ferricyanide + H2O
benzoate + 2 ferrocyanide + 2 H+
show the reaction diagram
-
-
-
-
-
benzaldehyde + 2 ferricyanide + H2O
benzoate + 2 ferrocyanide + 2 H+
show the reaction diagram
-
-
-
-
?
benzaldehyde + 2 ferricyanide + H2O
benzoate + 2 ferrocyanide + 2 H+
show the reaction diagram
-
-
-
-
?
benzaldehyde + 2 ferricyanide + H2O
benzoate + 2 ferrocyanide + 2 H+
show the reaction diagram
-
-
-
-
?
benzaldehyde + 2 ferricyanide + H2O
benzoate + 2 ferrocyanide + 2 H+
show the reaction diagram
Mus musculus CD-1, Mus musculus DBA/2
-
-
-
-
?
benzaldehyde + 2,6-dichlorophenol indophenol
?
show the reaction diagram
G3X982
natural electron acceptor of enzyme is molecular oxygen, DCPIP i.e., 2,6-dichlorophenol indophenol
-
-
?
benzaldehyde + H2O + O2
benzoic acid + H2O2
show the reaction diagram
-
-
-
-
?
benzaldehyde + H2O + O2
benzoic acid + H2O2
show the reaction diagram
-
-
-
-
?
benzaldehyde + H2O + O2
benzoic acid + H2O2
show the reaction diagram
-
-
-
-
?
benzaldehyde + H2O + O2
benzoic acid + H2O2
show the reaction diagram
-
-
-
-
?
benzaldehyde + H2O + O2
benzoic acid + H2O2
show the reaction diagram
-
-
-
-
?
benzaldehyde + H2O + O2
benzoic acid + H2O2
show the reaction diagram
-
-
-
-
?
benzaldehyde + H2O + O2
benzoic acid + H2O2
show the reaction diagram
-
-
-
-
?
benzaldehyde + H2O + O2
benzoic acid + H2O2
show the reaction diagram
-
-
-
-
?
benzaldehyde + H2O + O2
benzoic acid + H2O2
show the reaction diagram
synthetic construct
-
-
-
-
?
benzaldehyde + H2O + O2
benzoic acid + H2O2
show the reaction diagram
-
-
-
-
?
benzaldehyde + H2O + O2
benzoic acid + H2O2
show the reaction diagram
Q2QB49, Q2QB50
-
-
-
?
benzaldehyde + H2O + O2
benzoic acid + H2O2
show the reaction diagram
S5FPI8
-
-
-
?
benzaldehyde + H2O + O2
benzoic acid + H2O2
show the reaction diagram
-
diphenylsulfoxide as electron acceptor under anaerobic conditions and ferricyanide and 2,6-dichlorophenol-indophenol as electron acceptor under aerobic conditions
-
-
?
benzaldehyde + H2O + O2
benzoic acid + H2O2
show the reaction diagram
-
chromate as electron acceptor
-
-
-
benzaldehyde + H2O + O2
benzoic acid + H2O2
show the reaction diagram
-
2,6-dichlorophenol-indophenol also as electron acceptor
-
-
?
benzaldehyde + H2O + O2
benzoic acid + H2O2
show the reaction diagram
-
2,6-dichlorophenol-indophenol also as electron acceptor
-
-
?
benzaldehyde + H2O + O2
benzoic acid + H2O2
show the reaction diagram
-
2,6-dichlorophenol-indophenol also as electron acceptor
-
-
?
benzaldehyde + H2O + O2
benzoic acid + H2O2
show the reaction diagram
Streptomyces rimosus ATCC10970
-
-
-
-
?
benzaldehyde + H2O + O2
benzoic acid + H2O2
show the reaction diagram
Desulfovibrio aminophilus DSM12254
-
-
-
-
?
benzaldehyde + H2O + O2
benzoate + H2O2
show the reaction diagram
-
-
-
-
?
benzaldehyde + H2O + O2
benzoate + H2O2
show the reaction diagram
-
-
-
-
?
benzaldehyde + H2O + O2
benzoate + H2O2
show the reaction diagram
-
-
-
-
?
benzaldehyde + H2O + O2
benzoate + H2O2
show the reaction diagram
Q5QE78, Q5QE79, Q5QE80, Q9Z0U5
-
-
-
?
benzaldehyde + H2O + O2
benzoate + H2O2
show the reaction diagram
-
-
-
-
?
benzaldehyde + H2O + O2
benzoate + H2O2
show the reaction diagram
-
-
-
-
?
benzaldehyde + H2O + O2
benzoate + H2O2
show the reaction diagram
Q9NCL9
-
-
-
?
benzaldehyde + H2O + O2
benzoate + H2O2
show the reaction diagram
-
-
-
-
?
benzaldehyde + H2O + O2
benzoate + H2O2
show the reaction diagram
-
130% of activity with formaldehyde
-
-
?
benzaldehyde + H2O + O2
benzoate + H2O2
show the reaction diagram
-
160% of activity with formaldehyde
-
-
?
benzaldehyde + H2O + O2
benzoate + H2O2
show the reaction diagram
-
41% of activity with n-hexylaldehyde
-
-
?
benzaldehyde + H2O + O2
benzoate + H2O2
show the reaction diagram
-
410% of activity with formaldehyde
-
-
?
benzaldehyde + H2O + O2
benzoate + H2O2
show the reaction diagram
-
-
-
-
?
benzaldehyde + O2 + H2O
benzoate + H2O2
show the reaction diagram
-
-
-
-
?
benzaldehyde + O2 + H2O
benzoate + H2O2
show the reaction diagram
-
-
-
-
?
benzaldehyde + O2 + H2O
benzoate + H2O2
show the reaction diagram
Mus musculus CD-1
-
-
-
-
?
benzaldehyde + O2 + H2O
benzoate + H2O2
show the reaction diagram
Pseudomonas sp. KY 469
-
-
-
-
?
benzaldehyde + O2 + H2O
benzoate + H2O2
show the reaction diagram
Mus musculus DBA/2
-
-
-
-
?
benzaldehyde + O2 + H2O
benzoate + H2O2
show the reaction diagram
Pseudomonas sp. KY 4690
-
-
-
-
?
benzamidoxime + H2O + 2-hydroxypyrimidine
benzamidine + ?
show the reaction diagram
-
other electron acceptors are N-methylnicotinamide, butyraldehyde and benzaldehyde
-
-
?
BIBX1382 + H2O + O2
BIBU1476 + ?
show the reaction diagram
-
high hepatic clearance of 17 to 18 ml/(min * kg)
product identification by high-resolution mass spectrometry
-
?
bombykal + H2O + O2
?
show the reaction diagram
A8TUB4, A8TUC0
-
-
-
?
bombykal + H2O + O2
?
show the reaction diagram
Bombyx mori Nistari
A8TUB4, A8TUC0
-
-
-
?
butanal + 2 ferricyanide + H2O
butanoate + 2 ferrocyanide + 2 H+
show the reaction diagram
-
-
-
-
?
butanal + 2 ferricyanide + H2O
butanoate + 2 ferrocyanide + 2 H+
show the reaction diagram
Mus musculus CD-1, Mus musculus DBA/2
-
-
-
-
?
butanal + H2O + O2
butyric acid + H2O2
show the reaction diagram
-
-
-
-
?
butanal + H2O + O2
butyric acid + H2O2
show the reaction diagram
-
-
-
-
?
butanal + H2O + O2
butyric acid + H2O2
show the reaction diagram
-
2,6-dichlorophenol-indophenol as electron acceptor
-
-
?
butanal + H2O + O2
butyric acid + H2O2
show the reaction diagram
-
ferricyanide and 2,6-dichlorophenol-indophenol as electron acceptor under aerobic conditions
-
-
?
butanal + H2O + O2
butyric acid + H2O2
show the reaction diagram
-
ferricyanide as electron donor under aerobic conditions
-
-
?
butylaldehyde + H2O + O2
butanoate + H2O2
show the reaction diagram
-
260% of activity with formaldehyde
-
-
?
butylaldehyde + H2O + O2
butanoate + H2O2
show the reaction diagram
-
31% of activity with formaldehyde
-
-
?
butylaldehyde + H2O + O2
butanoate + H2O2
show the reaction diagram
-
360% of activity with formaldehyde
-
-
?
butyraldehyde + H2O + O2
butanoate + H2O2
show the reaction diagram
-
-
-
-
?
carbazeran + H2O + O2
?
show the reaction diagram
-
-
-
-
?
carbazeran + H2O + O2
carbazeran phthalazinone + ?
show the reaction diagram
-
high hepatic clearance of 17 to 18 ml/(min * kg)
product identification by high-resolution mass spectrometry
-
?
chloroacetaldehyde + H2O + O2
chloroacetic acid + H2O2
show the reaction diagram
-
2,6-dichlorophenol-indophenol as electron acceptor
-
-
?
cinchonidine + H2O + O2
?
show the reaction diagram
-
rabbit AOX1 has an extremely high Vmax value toward cinchonidine
-
-
?
cinchonidine + H2O + O2
?
show the reaction diagram
-
wild type monkey AOX1 is not able to oxidize chinchonidine, but mutant enzyme V1085A does
-
-
?
cinnamaldehyde + H2O + O2
cinnamic acid + H2O2
show the reaction diagram
-
170% of activity with formaldehyde
-
-
?
cinnamaldehyde + H2O + O2
cinnamic acid + H2O2
show the reaction diagram
-
240% of activity with formaldehyde
-
-
?
cinnamaldehyde + H2O + O2
cinnamic acid + H2O2
show the reaction diagram
-
410% of activity with formaldehyde
-
-
?
cinnamaldehyde + H2O + O2
cinnamic acid + H2O2
show the reaction diagram
-
44.2% of the rate with benzaldehyde
-
-
?
cis-5-fluoro-2-methyl-1-[p-(methylsulfinyl)benzylindenyl]indene-3-acetic acid + electron acceptor + H2O
5-fluoro-2-methyl-1-[p-(methylthio)benzylindenyl]indene-3-acetic acid + ?
show the reaction diagram
-
N1-methylnicotinamide and acetaldehyde as electron acceptors under anaerobic conditions
-
-
?
citral + H2O + O2
(2E)-3,7-dimethylocta-2,6-dienoic acid + H2O2
show the reaction diagram
-
210% of activity with formaldehyde
-
-
?
citral + H2O + O2
(2E)-3,7-dimethylocta-2,6-dienoic acid + H2O2
show the reaction diagram
-
260% of activity with formaldehyde
-
-
?
citral + H2O + O2
(2E)-3,7-dimethylocta-2,6-dienoic acid + H2O2
show the reaction diagram
-
430% of activity with formaldehyde
-
-
?
citral + H2O + O2
(2E)-3,7-dimethylocta-2,6-dienoic acid + H2O2
show the reaction diagram
S5FPI8
low AtraAOX2 activity
-
-
?
citral + H2O + O2
citrate + H2O2
show the reaction diagram
-
13% of activity with n-hexylaldehyde
-
-
?
clothianidin + H2O + O2
? + H2O2
show the reaction diagram
-
i.e. (2E)-2-[([(2-chloro-1,3-thiazol-5-yl)methyl]amino)(methylamino)methylene]-1-hydroxy-1-oxodiazanium, enzyme system coupled with Drosophila nicotinic acetylcholine receptor, strong inactivation of neonicotinoid insecticide substrate
-
-
?
clothianidin + NMNH
nitroso-clothianidin + amino-clothianidin + ?
show the reaction diagram
-
-
-
-
?
crotonaldehyde + H2O + O2
crotonic acid + H2O2
show the reaction diagram
-
2,6-dichlorophenol-indophenol as electron acceptor
-
-
?
crotonaldehyde + H2O + O2
crotonic acid + H2O2
show the reaction diagram
-
230% of activity with formaldehyde
-
-
?
crotonaldehyde + H2O + O2
crotonic acid + H2O2
show the reaction diagram
-
340% of activity with formaldehyde
-
-
?
crotonaldehyde + H2O + O2
crotonic acid + H2O2
show the reaction diagram
-
61% of activity with formaldehyde
-
-
?
crotonaldehyde + H2O + O2
crotonate + H2O2
show the reaction diagram
-
32% of activity with n-hexylaldehyde
-
-
?
decylaldehyde + H2O + O2
decanoate + H2O2
show the reaction diagram
-
400% of activity with formaldehyde
-
-
?
decylaldehyde + H2O + O2
decanoate + H2O2
show the reaction diagram
-
41% of activity with formaldehyde
-
-
?
decylaldehyde + H2O + O2
decanoate + H2O2
show the reaction diagram
-
52% of activity with formaldehyde
-
-
?
desmethyl-thiamethoxam + H2O + O2
? + H2O2
show the reaction diagram
-
i.e. (4E)-3-[(2-chloro-1,3-thiazol-5-yl)methyl]-N-nitro-1,3,5-oxadiazinan-4-imine, enzyme system coupled with Drosophila nicotinic acetylcholine receptor, strong inactivation of neonicotinoid insecticide substrate
-
-
?
dibenzyl sulfoxide + acetaldehyde + H2O
?
show the reaction diagram
-
under anaerobic conditions
-
-
?
dinotefuran + H2O + O2
? + H2O2
show the reaction diagram
-
i.e. 1-methyl-2-nitro-3-(tetrahydrofuran-3-ylmethyl)guanidine, enzyme system coupled with Drosophila nicotinic acetylcholine receptor, strong inactivation of neonicotinoid insecticide substrate
-
-
?
diphenyl sulfoxide + acetaldehyde + H2O
?
show the reaction diagram
-
-
-
-
-
diphenyl sulfoxide + acetaldehyde + H2O
?
show the reaction diagram
-
under anaerobic conditions
-
-
?
DL-glyceraldehyde + H2O + O2
DL-glycerate + H2O2
show the reaction diagram
-
28% of activity with formaldehyde
-
-
?
DL-glyceraldehyde + H2O + O2
DL-glycerate + H2O2
show the reaction diagram
-
5% of activity with n-hexylaldehyde
-
-
?
DL-glyceraldehyde + H2O + O2
DL-glycerate + H2O2
show the reaction diagram
-
73% of activity with formaldehyde
-
-
?
DL-glyceraldehyde + H2O + O2
DL-glycerate + H2O2
show the reaction diagram
-
77% of activity with formaldehyde
-
-
?
DL-glyceraldehyde + O2 + H2O
glycerate + H2O2
show the reaction diagram
Pseudomonas sp., Pseudomonas sp. KY 469, Pseudomonas sp. KY 4690
-
-
-
-
?
electron donor + nicotinamide N-oxide
nicotinamide + electron acceptor
show the reaction diagram
-
2-hydroxypyrimidine as electron acceptor
-
-
?
electron donor + nicotinamide N-oxide
nicotinamide + electron acceptor
show the reaction diagram
-
electron donors are 2-hydroxypyrimidine, N1-methylnicotinamide, benzaldehyde or butyraldehyde
-
-
?
formaldehyde + H2O + O2
formic acid + H2O2
show the reaction diagram
-
-
-
-
?
formaldehyde + H2O + O2
formic acid + H2O2
show the reaction diagram
-
2,6-dichlorophenol-indophenol as electron acceptor
-
-
?
formaldehyde + H2O + O2
formic acid + H2O2
show the reaction diagram
Streptomyces rimosus ATCC10970
-
-
-
-
?
formaldehyde + H2O + O2
formate + H2O2
show the reaction diagram
-
-
-
-
?
formaldehyde + H2O + O2
formate + H2O2
show the reaction diagram
-
2% of activity with n-hexylaldehyde, aldehyde oxidase might be effective for the removal of formaldehyde contained in wastewater
-
-
?
formaldehyde + O2 + H2O
formate + H2O2
show the reaction diagram
Pseudomonas sp., Pseudomonas sp. KY 469, Pseudomonas sp. KY 4690
-
-
-
-
?
formate + O2
CO2 + H2O2
show the reaction diagram
-
-
-
-
?
furfural + H2O + O2
2-furoic acid + H2O2
show the reaction diagram
-
2,6-dichlorophenol-indophenol as electron acceptor
-
-
?
glutaraldehyde + H2O + O2
glutarate + H2O2
show the reaction diagram
-
18% of activity with formaldehyde
-
-
?
glutaraldehyde + H2O + O2
glutarate + H2O2
show the reaction diagram
-
20% of activity with formaldehyde
-
-
?
glutaraldehyde + H2O + O2
glutarate + H2O2
show the reaction diagram
-
30% of activity with n-hexylaldehyde
-
-
?
glutaraldehyde + H2O + O2
glutarate + H2O2
show the reaction diagram
-
64% of activity with formaldehyde
-
-
?
glutaraldehyde + H2O + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
glutaraldehyde + O2 + H2O
glutarate + H2O2
show the reaction diagram
-
-
-
-
?
glyceraldehyde + H2O + O2
2,3-dihydroxypropanoic acid + H2O2
show the reaction diagram
-
ferricyanide and 2,6-dichlorophenol-indophenol as electron acceptor under aerobic conditions
-
-
?
glycoaldehyde + H2O + O2
hydroxyacetic acid + H2O2
show the reaction diagram
-
ferricyanide and 2,6-dichlorophenol-indophenol as electron acceptor under aerobic conditions
-
-
?
glyoxal + H2O + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
glyoxal + H2O + O2
glyoxylate + H2O2
show the reaction diagram
-
-
-
-
?
heptaldehyde + H2O + O2
heptanoic acid + H2O2
show the reaction diagram
-
-
-
-
?
heptanal + H2O + O2
heptanoic acid + H2O2
show the reaction diagram
S5FPI8
-
-
-
?
heptanal + H2O + O2
heptanoic acid + H2O2
show the reaction diagram
-
ferricyanide and 2,6-dichlorophenol-indophenol as electron acceptor under aerobic conditions
-
-
?
heptylaldehyde + H2O + O2
heptanoate + H2O2
show the reaction diagram
-
350% of activity with formaldehyde
-
-
?
heptylaldehyde + H2O + O2
heptanoate + H2O2
show the reaction diagram
-
480% of activity with formaldehyde
-
-
?
heptylaldehyde + H2O + O2
heptanoate + H2O2
show the reaction diagram
-
76% of activity with formaldehyde
-
-
?
hexanal + H2O + O2
hexanoic acid + H2O2
show the reaction diagram
-
-
-
-
ir
hexanal + H2O + O2
hexanoic acid + H2O2
show the reaction diagram
-
-
-
-
?
hexanal + H2O + O2
hexanoic acid + H2O2
show the reaction diagram
S5FPI8
-
-
-
?
hexylaldehyde + H2O + O2
hexanoate + H2O2
show the reaction diagram
-
-
-
-
?
hexylaldehyde + H2O + O2
hexanoate + H2O2
show the reaction diagram
-
360% of activity with formaldehyde
-
-
?
hexylaldehyde + H2O + O2
hexanoate + H2O2
show the reaction diagram
-
430% of activity with formaldehyde
-
-
?
hexylaldehyde + H2O + O2
hexanoate + H2O2
show the reaction diagram
-
74% of activity with formaldehyde
-
-
?
hexylaldehyde + H2O + O2
hexanoate + H2O2
show the reaction diagram
-
-
-
-
?
hypoxanthine + O2 + H2O
xanthine + H2O2
show the reaction diagram
-
22-26% of the activity compared to purine
-
?
imidacloprid + H2O + O2
? + H2O2
show the reaction diagram
-
i.e. (2E)-1-[(6-chloropyridin-3-yl)methyl]-N-nitroimidazolidin-2-imine, enzyme system coupled with Drosophila nicotinic acetylcholine receptor, strong inactivation of neonicotinoid insecticide substrate
-
-
?
imidacloprid + H2O + O2
nitroso-imidacloprid
show the reaction diagram
-
-
without addition of an electron donor, product is nitroso-imidacloprid
-
?
imidacloprid + H2O + O2 + N-methylnicotinamide
nitroso-imidacloprid + amino-imidacloprid + H2O2 + ?
show the reaction diagram
Q9Z0U5
-
-
-
-
imidacloprid + H2O + O2 + N-methylnicotinamide
nitroso-imidacloprid + amino-imidacloprid + H2O2 + ?
show the reaction diagram
-
-
-
-
-
imidacloprid + H2O + O2 + N-methylnicotinamide
nitroso-imidacloprid + amino-imidacloprid + H2O2 + ?
show the reaction diagram
-
-
-
-
-
imidacloprid + H2O + O2 + N-methylnicotinamide
nitroso-imidacloprid + amino-imidacloprid + H2O2 + ?
show the reaction diagram
-
-
in presence of N-methylnicotinamide, ratio of amino- to nitroso-products is 10 to 269
-
?
imidacloprid + H2O + O2 + N-methylnicotinamide
nitroso-imidacloprid + amino-imidacloprid + H2O2 + ?
show the reaction diagram
-
-
in presence of N-methylnicotinamide, ratio of amino- to nitroso-products is 2.0 to 1.8
-
?
imidacloprid + H2O + O2 + N-methylnicotinamide
nitroso-imidacloprid + amino-imidacloprid + H2O2 + ?
show the reaction diagram
-
-
in presence of N-methylnicotinamide, ratio of amino- to nitroso-products is 23 to 14
-
?
imidacloprid + H2O + O2 + N-methylnicotinamide
nitroso-imidacloprid + amino-imidacloprid + H2O2 + ?
show the reaction diagram
-
-
in presence of N-methylnicotinamide, ratio of amino- to nitroso-products is 26 to 50
-
?
imidacloprid + H2O + O2 + N-methylnicotinamide
nitroso-imidacloprid + amino-imidacloprid + H2O2 + ?
show the reaction diagram
-
-
in presence of N-methylnicotinamide, ratio of amino- to nitroso-products is 80 to 41
-
?
imidacloprid + NMNH
nitroso-imidacloprid + amino-imidacloprid + ?
show the reaction diagram
-
-
-
-
?
imipramine N-oxide + electron donor
imipramine + electron acceptor
show the reaction diagram
-
electron donors are 2-hydroxypyrimidine, N1-methylnicotinamide, benzaldehyde or butyraldehyde under anaerobic conditions
-
-
?
indol-3-carboxyaldehyde + H2O + O2
indol-3-carboxylic acid + H2O2
show the reaction diagram
-
100% of activity with formaldehyde
-
-
?
indol-3-carboxyaldehyde + H2O + O2
indol-3-carboxylic acid + H2O2
show the reaction diagram
-
20% of activity with formaldehyde
-
-
?
indol-3-carboxyaldehyde + H2O + O2
indol-3-carboxylic acid + H2O2
show the reaction diagram
-
8% of activity with formaldehyde
-
-
?
indole 3-acetaldehyde + H2O + O2
indole 3-acetic acid + H2O2
show the reaction diagram
-
2,6-dichlorophenol-indophenol also as electron acceptor
-
-
?
indole 3-acetaldehyde + H2O + O2
indole 3-acetic acid + H2O2
show the reaction diagram
-
48.0% of the rate with benzaldehyde
-
-
?
indole 3-carbaldehyde + H2O + O2
indole 3-carboxylate + H2O2
show the reaction diagram
-
-
-
-
?
indole 3-carbaldehyde + H2O + O2
indole 3-carboxylate + H2O2
show the reaction diagram
-
2,6-dichlorophenol-indophenol also as electron acceptor
-
-
?
indole-3-aldehyde + H2O + O2
indole-3-carboxylate + H2O2
show the reaction diagram
-
-
-
-
?
indole-3-aldehyde + H2O + O2
indole-3-carboxylate + H2O2
show the reaction diagram
B0LAZ9, B0LB00, B0LB01
-
-
-
?
indole-3-aldehyde + H2O + O2
indole-3-carboxylic acid + H2O2
show the reaction diagram
-
-
-
-
?
indole-3-aldehyde + H2O + O2
indole-3-carboxylic acid + H2O2
show the reaction diagram
-
-
-
-
?
indole-3-aldehyde + H2O + O2
indole-3-carboxylic acid + H2O2
show the reaction diagram
-
-
-
-
?
indole-3-aldehyde + H2O + O2
indole-3-carboxylic acid + H2O2
show the reaction diagram
-
-
-
-
?
indole-3-aldehyde + O2 + H2O
indole-3-carboxylate + H2O2
show the reaction diagram
-
-
-
-
?
indole-3-aldehyde + O2 + H2O
indole-3-carboxylate + H2O2
show the reaction diagram
-
-
-
-
?
isobutyraldehyde + H2O + O2
isobutyric acid + H2O2
show the reaction diagram
-
ferricyanide as electron acceptor under aerobic conditions
-
-
?
isovaleraldehyde + H2O + O2
isovaleric acid + H2O2
show the reaction diagram
-
ferricyanide as electron acceptor under aerobic conditions
-
-
?
L-methionine sulfoxide + acetaldehyde + H2O
?
show the reaction diagram
-
under anaerobic conditions
-
-
?
m-tolualdehyde + H2O + O2
m-methylbenzoic acid + H2O2
show the reaction diagram
-
-
-
-
?
methotrexate + H2O + O2
?
show the reaction diagram
-
-
-
-
?
methotrexate + H2O + O2
?
show the reaction diagram
Q5QE78, Q5QE79, Q5QE80, Q9Z0U5
-
-
-
?
n-alkanal + H2O + O2
n-alkanoic acid + H2O2
show the reaction diagram
-
C5-C10, C12, C14 with decreasing activities
-
-
?
n-heptanal + H2O + O2
n-heptanoic acid + H2O2
show the reaction diagram
-
-
-
-
?
n-heptylaldehyde + H2O + O2
heptanoate + H2O2
show the reaction diagram
-
78% of activity with n-hexylaldehyde
-
-
?
n-heptylaldehyde + O2 + H2O
heptanoate + H2O2
show the reaction diagram
-
-
-
-
?
n-hexanal + H2O + O2
n-hexanoic acid + H2O2
show the reaction diagram
-
-
-
-
?
n-hexylaldehyde + H2O + O2
hexanoate + H2O2
show the reaction diagram
-
-
-
-
?
N-methyl-5,6-benzoquinolinium + H2O + ferricyanide
?
show the reaction diagram
-
-
-
-
?
N-methylimidacloprid + H2O + O2
? + H2O2
show the reaction diagram
-
i.e. (2E)-1-[(6-chloropyridin-3-yl)methyl]-3-methyl-N-nitroimidazolidin-2-imine, enzyme system coupled with Drosophila nicotinic acetylcholine receptor, little inactivation of neonicotinoid insecticide substrate
-
-
?
N-methylnicotinamide + electron acceptor + H2O
?
show the reaction diagram
-
electron acceptors i.e.: dichlorophenolindophenol, nitro blue tetrazolium, ferricyanide, diaphorase activity
-
-
?
N-methylphenanthridinium + H2O + ferricyanide
N-methyl-6-phenanthridone + ferrocyanide
show the reaction diagram
-
-
-
-
?
N-methylphthalazinium + O2 + H2O
?
show the reaction diagram
-
-
-
-
?
N-methylquinolinium + H2O + electron donor
N-methyl-4-quinolone and N-methyl-2-quinolone + electron acceptor
show the reaction diagram
-
-
-
-
?
N-methylquinolinium + H2O + electron donor
N-methyl-4-quinolone and N-methyl-2-quinolone + electron acceptor
show the reaction diagram
-
electron donors are ferricyanide and cytochrome c
-
-
?
n-pentanal + H2O + O2
n-pentanoic acid + H2O2
show the reaction diagram
-
-
-
-
?
N-phenylquinolinium + H2O + electron donor
N-phenyl-4-quinolone and N-phenyl-2-quinolone + electron acceptor
show the reaction diagram
-
-
-
-
?
N-phenylquinolinium + H2O + electron donor
N-phenyl-4-quinolone and N-phenyl-2-quinolone + electron acceptor
show the reaction diagram
-
electron donors are ferricyanide and cytochrome c
-
-
?
n-valeraldehyde + O2 + H2O
valerate + H2O2
show the reaction diagram
-
-
-
-
?
N-[(2'-dimethylamino)ethyl]acridine-4-carboxamide + H2O + O2
?
show the reaction diagram
-
-
-
-
?
N-[(2-dimethylamino)ethyl] acridine-4-carboxamide + H2O + O2
N-[(2-dimethylamino)ethyl] acridine-4-carboxamide-9(10H)-acridone + H2O2
show the reaction diagram
-
-
-
-
?
N-[(2-dimethylamino)ethyl] acridine-4-carboxamide + H2O + O2
N-[(2-dimethylamino)ethyl] acridine-4-carboxamide-9(10H)-acridone + H2O2
show the reaction diagram
-
-
-
-
?
N-[(2-dimethylamino)ethyl]acridine-4-carboxamide + H2O + O2
DACA-9(10H)-acridone + ?
show the reaction diagram
-
N-[(2-dimethylamino)ethyl]acridine-4-carboxamide i.e. DACA, an experimental antitumor agent
-
-
?
N-[2-(dimethylamino)ethyl]acridine-4-carboxamide + H2O + O2
N-[2-(dimethylamino)ethyl]acridine-4-carboxamide-9-(10H)-acridone + H2O2
show the reaction diagram
-
antitumor drug
-
-
?
N1-methylnicotinamide + 2,6-dichlorophenol indophenol
?
show the reaction diagram
G3X982
natural electron acceptor of enzyme is molecular oxygen, DCPIP i.e., 2,6-dichlorophenol indophenol
-
-
?
N1-methylnicotinamide + H2O + O2
N1-methyl-2-pyridone-5-carboxamide + N1-methyl-4-pyridone-3-carboxamide + H2O2
show the reaction diagram
-
-
-
-
-
N1-methylnicotinamide + H2O + O2
N1-methyl-2-pyridone-5-carboxamide + N1-methyl-4-pyridone-3-carboxamide + H2O2
show the reaction diagram
-
-
-
-
?
N1-methylnicotinamide + H2O + O2
N1-methyl-2-pyridone-5-carboxamide + N1-methyl-4-pyridone-3-carboxamide + H2O2
show the reaction diagram
-
-
-
-
?
N1-methylnicotinamide + H2O + O2
N1-methyl-2-pyridone-5-carboxamide + N1-methyl-4-pyridone-3-carboxamide + H2O2
show the reaction diagram
-
-
-
-
?
N1-methylnicotinamide + H2O + O2
N1-methyl-2-pyridone-5-carboxamide + N1-methyl-4-pyridone-3-carboxamide + H2O2
show the reaction diagram
Q9Z0U5
-
-
-
?
N1-methylnicotinamide + H2O + O2
N1-methyl-2-pyridone-5-carboxamide + N1-methyl-4-pyridone-3-carboxamide + H2O2
show the reaction diagram
-
-
-
-
?
N1-methylnicotinamide + H2O + O2
N1-methyl-2-pyridone-5-carboxamide + N1-methyl-4-pyridone-3-carboxamide + H2O2
show the reaction diagram
Q5QE78, Q5QE79, Q5QE80, Q9Z0U5
-
-
-
?
N1-methylnicotinamide + H2O + O2
N1-methyl-2-pyridone-5-carboxamide + N1-methyl-4-pyridone-3-carboxamide + H2O2
show the reaction diagram
-
-
-
-
?
N1-methylnicotinamide + H2O + O2
N1-methyl-2-pyridone-5-carboxamide + N1-methyl-4-pyridone-3-carboxamide + H2O2
show the reaction diagram
-
-
-
-
?
N1-methylnicotinamide + H2O + O2
N1-methyl-2-pyridone-5-carboxamide + N1-methyl-4-pyridone-3-carboxamide + H2O2
show the reaction diagram
-
-
-
-
?
N1-methylnicotinamide + H2O + O2
N1-methyl-2-pyridone-5-carboxamide + N1-methyl-4-pyridone-3-carboxamide + H2O2
show the reaction diagram
P80456
-
-
-
?
N1-methylnicotinamide + H2O + O2
N1-methyl-2-pyridone-5-carboxamide + N1-methyl-4-pyridone-3-carboxamide + H2O2
show the reaction diagram
-
2,6-dichlorophenol-indophenol as electron acceptor
-
-
?
N1-methylnicotinamide + H2O + O2
N1-methyl-2-pyridone-5-carboxamide + N1-methyl-4-pyridone-3-carboxamide + H2O2
show the reaction diagram
-
potassium ferricyanide acts as electron acceptor
-
-
-
N1-methylnicotinamide + H2O + O2
N1-methyl-2-pyridone-5-carboxamide + N1-methyl-4-pyridone-3-carboxamide + H2O2
show the reaction diagram
-
potassium ferricyanide acts as electron acceptor
-
-
?
N1-methylnicotinamide + H2O + O2
N1-methyl-2-pyridone-5-carboxamide + N1-methyl-4-pyridone-3-carboxamide + H2O2
show the reaction diagram
-
diphenylsulfoxide as electron acceptor under anaerobic conditions and ferricyanide as electron acceptor under aerobic conditions
-
-
?
N1-methylnicotinamide + H2O + O2
N1-methyl-2-pyridone-5-carboxamide + N1-methyl-4-pyridone-3-carboxamide + H2O2
show the reaction diagram
-
chromate as electron acceptor, reduction of chromate
-
-
-
N1-methylnicotinamide + H2O + O2
N1-methyl-2-pyridone-5-carboxamide + N1-methyl-4-pyridone-3-carboxamide + H2O2
show the reaction diagram
-
cytochrome c reduction under aerobic conditions
-
-
?
N1-methylnicotinamide + H2O + O2
N1-methyl-2-pyridone-5-carboxamide + N1-methyl-4-pyridone-3-carboxamide + ?
show the reaction diagram
-
variations in the ratio of the amount of pyridones to the total amount of NMN and pyridones in urine are closely related to the enzyme activity
-
-
-
N1-propylnicotinamide + H2O + O2
N1-propyl-2-pyridone-5-carboxamide + N1-propyl-4-pyridone-3-carboxamide + H2O2
show the reaction diagram
-
-
-
-
?
NADH + nitrite + H2O + O2
? + nitric oxide
show the reaction diagram
-
in the presence of typical aldehyde substrates like 4-(dimethylamino)cinnamaldehyde or NADH, aldehyde oxidase reduces nitrite to nitric oxide. NADH reacts with aldehyde oxidase at the FAD site of the enzyme
-
-
?
nitenpyram + H2O + O2
? + H2O2
show the reaction diagram
-
i.e. (E)-N-[(6-chloropyridin-3-yl)methyl]-N-ethyl-N'-methyl-2-nitroethylene-1,1-diamine, enzyme system coupled with Drosophila nicotinic acetylcholine receptor, partial inactivation of neonicotinoid insecticide substrate
-
-
?
nitenpyram + NMNH
nitroso-nitenpyram + amino-nitenpyram + ?
show the reaction diagram
-
-
-
-
?
nithiazine + H2O + O2
? + H2O2
show the reaction diagram
-
i.e. (2Z)-2-(nitromethylene)-1,3-thiazinane, enzyme system coupled with Drosophila nicotinic acetylcholine receptor, partial inactivation of neonicotinoid insecticide substrate
-
-
?
nonanal + H2O + O2
nonanoic acid + H2O2
show the reaction diagram
S5FPI8
-
-
-
?
o-tolualdehyde + H2O + O2
o-methylbenzoic acid + H2O2
show the reaction diagram
-
-
-
-
?
O6-benzylguanine + H2O + O2
O6-benzyl-8-oxoguanine benzylguanine + ?
show the reaction diagram
-
hepatic clearance of 11.2 to 12.8 ml/(min * kg)
product identification by high-resolution mass spectrometry
-
?
octylaldehyde + H2O + O2
octanoate + H2O2
show the reaction diagram
-
350% of activity with formaldehyde
-
-
?
octylaldehyde + H2O + O2
octanoate + H2O2
show the reaction diagram
-
430% of activity with formaldehyde
-
-
?
octylaldehyde + H2O + O2
octanoate + H2O2
show the reaction diagram
-
64% of activity with formaldehyde
-
-
?
octylaldehyde + O2 + H2O
octanoate + H2O2
show the reaction diagram
-
-
-
-
?
p-aminobenzamidine + H2O + O2
?
show the reaction diagram
-
-
-
-
?
p-anisaldehyde + H2O + O2
p-anisic acid + H2O2
show the reaction diagram
-
180% of activity with formaldehyde
-
-
?
p-anisaldehyde + H2O + O2
p-anisic acid + H2O2
show the reaction diagram
-
190% of activity with formaldehyde
-
-
?
p-anisaldehyde + H2O + O2
p-anisic acid + H2O2
show the reaction diagram
-
500% of activity with formaldehyde
-
-
?
p-anisaldehyde + H2O + O2
p-anisic acid + H2O2
show the reaction diagram
-
40.2% of the rate with benzaldehyde
-
-
?
p-dimethyl aminocinnamaldehyde + H2O + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
p-hydroxybenzaldehyde + H2O + O2
p-hydroxybenzoate + H2O2
show the reaction diagram
-
-
-
-
?
p-hydroxybenzaldehyde + H2O + O2
p-hydroxybenzoate + H2O2
show the reaction diagram
-
200% of activity with formaldehyde
-
-
?
p-hydroxybenzaldehyde + H2O + O2
p-hydroxybenzoate + H2O2
show the reaction diagram
-
250% of activity with formaldehyde
-
-
?
p-hydroxybenzaldehyde + H2O + O2
p-hydroxybenzoate + H2O2
show the reaction diagram
-
320% of activity with formaldehyde
-
-
?
p-hydroxybenzaldehyde + H2O + O2
p-hydroxybenzoate + H2O2
show the reaction diagram
-
54.8% of the rate with benzaldehyde
-
-
?
p-methoxybenzaldehyde + H2O + O2
p-methoxybenzoate + H2O2
show the reaction diagram
-
-
-
-
?
p-tolualdehyde + H2O + O2
p-methylbenzoic acid + H2O2
show the reaction diagram
-
-
-
-
?
PF-4217903 + H2O + O2
?
show the reaction diagram
-
-
-
-
?
PF-945863 + H2O + O2
?
show the reaction diagram
-
-
-
-
?
phenanthrene-9-carboxaldehyde + H2O + O2
phenanthrene-9-carboxylate + H2O2
show the reaction diagram
-
-
-
-
?
phenanthridine + H2O + O2
6-phenanthridone + H2O2
show the reaction diagram
O54754
-
-
-
?
phenanthridine + H2O + O2
6-phenantridone + H2O2
show the reaction diagram
-
-
-
-
?
phenanthridine + H2O + O2
6-phenantridone + H2O2
show the reaction diagram
P48034
-
-
-
?
phenanthridine + H2O + O2
6-phenantridone + H2O2
show the reaction diagram
-
products are about 85-90% hydrogen peroxide and 6-10% superoxide anion
-
-
?
phenanthridine + H2O + O2
6-phenantridone + H2O2
show the reaction diagram
-
oxidation of vanillin is more sensitive to inhibition by flavonoids than that of phenanthridine
-
-
?
phenanthridine + H2O + O2
phenanthridinone + H2O2
show the reaction diagram
-
-
-
-
?
phenanthridine + H2O + O2
?
show the reaction diagram
G3X982
-
-
-
?
phenanthridine + H2O + O2
?
show the reaction diagram
P80456
activity of rabbit liver aldehyde oxidase characterized in nine water-miscible organic mixtures i.e. phosphate buffer mixed with N-N-dimethylformamide, acetonitrile, tetrahydrofuran, 1-propanol, 2-propanol, ethanol, pyridine, dioxane, and methanol
-
-
?
phenanthridine + H2O + O2
?
show the reaction diagram
Rattus norvegicus, Rattus norvegicus SpragueDawley
-
inhibitory effects of 15 flavonoids on the activity of rat liver aldehyde oxidase assessed, glycosylated flavonoids show relatively weaker inhibition, quantitative structureactivity relationship studies performed to elucidate the important structural properties responsible for the observed inhibitory effects
-
-
?
phenanthridine + O2 + H2O
?
show the reaction diagram
-
-
-
-
?
phenothiazine sulfoxide + acetaldehyde + H2O
?
show the reaction diagram
-
under anaerobic conditions
-
-
?
phenylacetaldehyde + H2O + O2
phenylacetic acid + H2O2
show the reaction diagram
-
ferricyanide as electron acceptor under aerobic conditions
-
-
?
phenylacetaldehyde + H2O + O2
phenylacetic acid + H2O2
show the reaction diagram
-
2,6-dichlorophenol-indophenol as electron acceptor
-
-
?
phenylacetaldehyde + H2O + O2
phenylacetate + H2O2
show the reaction diagram
-
130% of activity with formaldehyde
-
-
?
phenylacetaldehyde + H2O + O2
phenylacetate + H2O2
show the reaction diagram
-
230% of activity with formaldehyde
-
-
?
phenylacetaldehyde + H2O + O2
phenylacetate + H2O2
show the reaction diagram
-
26% of activity with formaldehyde
-
-
?
phthalaldehyde + H2O + O2
phthalic acid + H2O2
show the reaction diagram
-
13% of activity with n-hexylaldehyde
-
-
?
phthalazine + 2,6-dichlorophenol indophenol
?
show the reaction diagram
G3X982
natural electron acceptor of enzyme is molecular oxygen, DCPIP i.e., 2,6-dichlorophenol indophenol
-
-
?
phthalazine + ferricyanide
1-phthalazinone + ferrocyanide
show the reaction diagram
-
-
-
-
?
phthalazine + ferricyanide
?
show the reaction diagram
-
-
-
-
?
phthalazine + H2O + O2
1-phthalazinone + H2O2
show the reaction diagram
-
-
-
-
?
phthalazine + H2O + O2
1-phthalazinone + H2O2
show the reaction diagram
synthetic construct
-
-
-
-
?
phthalazine + H2O + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
phthalazine + H2O + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
phthalazine + O2 + H2O
1-phthalazinone + H2O2
show the reaction diagram
-
-
-
-
?
phthalazine + O2 + H2O
1-phthalazinone + H2O2
show the reaction diagram
-
-
-
-
?
propanal + H2O + O2
propanoic acid + H2O2
show the reaction diagram
S5FPI8
highest AtraAOX2 activity
-
-
?
propionaldehyde + H2O + O2
propionic acid + H2O2
show the reaction diagram
-
-
-
-
?
propionaldehyde + H2O + O2
propionic acid + H2O2
show the reaction diagram
-
-
-
-
?
propionaldehyde + H2O + O2
propionic acid + H2O2
show the reaction diagram
-
-
-
-
?
propionaldehyde + H2O + O2
propionic acid + H2O2
show the reaction diagram
-
2,6-dichlorophenol-indophenol as electron acceptor
-
-
?
propionaldehyde + H2O + O2
propionic acid + H2O2
show the reaction diagram
-
2,6-dichlorophenol-indophenol as electron acceptor
-
-
?
propionaldehyde + H2O + O2
propionic acid + H2O2
show the reaction diagram
-
2,6-dichlorophenol-indophenol as electron acceptor
-
-
?
propionaldehyde + H2O + O2
propionic acid + H2O2
show the reaction diagram
-
ferricyanide and 2,6-dichlorophenol-indophenol as electron acceptor under aerobic conditions
-
-
?
propionaldehyde + H2O + O2
propionic acid + H2O2
show the reaction diagram
-
-
-
-
?
propionaldehyde + H2O + O2
propionic acid + H2O2
show the reaction diagram
Desulfovibrio aminophilus DSM12254
-
-
-
-
?
propionaldehyde + H2O + O2
propionate + H2O2
show the reaction diagram
-
220% of activity with formaldehyde
-
-
?
propionaldehyde + H2O + O2
propionate + H2O2
show the reaction diagram
-
23% of activity with formaldehyde
-
-
?
propionaldehyde + H2O + O2
propionate + H2O2
show the reaction diagram
-
340% of activity with formaldehyde
-
-
?
protocatechualdehyde + H2O + O2
protocatechuic acid + H2O2
show the reaction diagram
-
2,6-dichlorophenol-indophenol also as electron acceptor
-
-
?
purine + O2 + H2O
? + H2O2
show the reaction diagram
-
best substrate tested
-
?
purine derivative + H2O + ferricyanide
oxidized purine derivative + ?
show the reaction diagram
-
various purine derivatives are listed, this enzyme catalyzes usually a ring methine group vicinal to a ring nitrogen
-
-
?
pyrazolo[3,4-d]pyrimidine + H2O + ferricyanide
oxidized pyrazolo[3,4-d]pyrimidine + ?
show the reaction diagram
-
various pyrazolo[3,4-d]pyrimidines are listed, this enzyme catalyzes usually a ring methine group vicinal to a ring nitrogen
-
-
?
pyridoxal + H2O + O2
4-pyridoxic acid + H2O2
show the reaction diagram
-
-
-
-
?
pyridoxal + H2O + O2
4-pyridoxic acid + H2O2
show the reaction diagram
O54754, Q5SGK3, Q6V956, Q8VJ15
-
-
-
?
quinazolin-4(3H)-one + H2O + O2
quinazoline-2,4(1H,3H)-dione + H2O2
show the reaction diagram
-
-
-
-
?
quinine + H2O + O2
?
show the reaction diagram
P80456
-
-
-
?
quinoline + H2O + electron donor
?
show the reaction diagram
-
-
-
-
?
quinoline + H2O + electron donor
?
show the reaction diagram
-
ferricyanide as electron donor under aerobic conditions
-
-
?
quinoline + H2O + electron donor
?
show the reaction diagram
-
chromate as electron donor
-
-
?
quinoline + H2O + electron donor
?
show the reaction diagram
-
ferricyanide and cytochrome c as electron donor
-
-
?
retinal + O2 + H2O
retinoate + H2O2
show the reaction diagram
-
-
-
?
retinal + O2 + H2O
retinoate + H2O2
show the reaction diagram
-
-
-
?
retinal + O2 + H2O
retinoate + H2O2
show the reaction diagram
-
-
-
?
retinal + O2 + H2O
retinoate + H2O2
show the reaction diagram
-
involved in vitamin A metabolism, increased activity of cells in hyperthyroidism, decreased activity of cells in hypothyroidism
-
?
retinal + O2 + H2O
retinoate + H2O2
show the reaction diagram
-
activity is dependent on vitamin A levels of tissues
-
?
retinal + O2 + H2O
retinoic acid + H2O2
show the reaction diagram
-
-
-
-
?
retinalaldehyde + O2 + H2O
retinoic acid + H2O2
show the reaction diagram
-
-
-
-
?
retinaldehyde + H2O + O2
retinoic acid + H2O2
show the reaction diagram
Q2QB49, Q2QB50
-
-
-
?
retinaldehyde + O2 + H2O
retinoic acid + H2O2
show the reaction diagram
-
-
-
-
?
salicylaldehyde + H2O + O2
salicylic acid + H2O2
show the reaction diagram
-
-
-
-
?
salicylaldehyde + H2O + O2
salicylic acid + H2O2
show the reaction diagram
-
ferricyanide as electron acceptor under aerobic conditions
-
-
?
salicylaldehyde + H2O + O2
salicylic acid + H2O2
show the reaction diagram
-
2,6-dichlorophenol-indophenol as electron acceptor
-
-
?
salicylaldehyde + H2O + O2
salicylic acid + H2O2
show the reaction diagram
-
120% of activity with formaldehyde
-
-
?
salicylaldehyde + H2O + O2
salicylic acid + H2O2
show the reaction diagram
-
22% of activity with formaldehyde
-
-
?
salicylaldehyde + H2O + O2
salicylic acid + H2O2
show the reaction diagram
-
260% of activity with formaldehyde
-
-
?
salicylaldehyde + H2O + O2
salicylic acid + H2O2
show the reaction diagram
-
8% of activity with n-hexylaldehyde
-
-
?
salicylaldehyde + H2O + O2
salicylic acid + H2O2
show the reaction diagram
Streptomyces rimosus ATCC10970
-
-
-
-
?
salicylaldehyde + H2O + O2
salicylic acid + H2O2
show the reaction diagram
Pseudomonas stutzeri 12695
-
8% of activity with n-hexylaldehyde
-
-
?
salicylaldoxime + H2O + 2-hydroxypyrimidine
salicylaldehyde + NH3
show the reaction diagram
-
other electron acceptors are N-methylnicotinamide, butyraldehyde and benzaldehyde
the corresponding ketimine is an intermediate
?
terephthalaldehyde + H2O + O2
terephthalic acid + H2O2
show the reaction diagram
-
45% of activity with n-hexylaldehyde
-
-
?
thiacloprid + H2O + O2
? + H2O2
show the reaction diagram
-
i.e. ((2Z)-3-[(6-chloropyridin-3-yl)methyl]-1,3-thiazolidin-2-ylidene)cyanamide, enzyme system coupled with Drosophila nicotinic acetylcholine receptor, little inactivation of neonicotinoid insecticide substrate
-
-
?
thiamethoxam + H2O + O2
? + H2O2
show the reaction diagram
-
i.e. (4E)-3-[(2-chloro-1,3-thiazol-5-yl)methyl]-5-methyl-N-nitro-1,3,5-oxadiazinan-4-imine, enzyme system coupled with Drosophila nicotinic acetylcholine receptor, strong inactivation of neonicotinoid insecticide substrate
-
-
?
tropylium tetrafluoroborate + H2O + ferricyanide
tropone + ?
show the reaction diagram
-
other electron acceptors as dichloroindophenol and chromate
-
-
?
valeraldehyde + H2O + O2
valeric acid + H2O2
show the reaction diagram
-
-
-
-
?
valeraldehyde + H2O + O2
valeric acid + H2O2
show the reaction diagram
-
ferricyanide as electron acceptor under aerobic conditions
-
-
?
valeraldehyde + H2O + O2
valeric acid + H2O2
show the reaction diagram
-
-
-
-
?
valeraldehyde + H2O + O2
valerate + H2O2
show the reaction diagram
-
350% of activity with formaldehyde
-
-
?
valeraldehyde + H2O + O2
valerate + H2O2
show the reaction diagram
-
360% of activity with formaldehyde
-
-
?
valeraldehyde + H2O + O2
valerate + H2O2
show the reaction diagram
-
56% of activity with formaldehyde
-
-
?
valeraldehyde + H2O + O2
valerate + H2O2
show the reaction diagram
-
67% of activity with n-hexylaldehyde
-
-
?
vanillin + H2O + O2
vanillic acid + H2O2
show the reaction diagram
-
-
-
-
?
vanillin + H2O + O2
vanillic acid + H2O2
show the reaction diagram
-
-
-
-
?
vanillin + H2O + O2
vanillic acid + H2O2
show the reaction diagram
-
-
-
-
?
vanillin + H2O + O2
vanillic acid + H2O2
show the reaction diagram
-
17% of activity with n-hexylaldehyde
-
-
?
vanillin + H2O + O2
vanillic acid + H2O2
show the reaction diagram
-
43.3% of the rate with benzaldehyde
-
-
?
vanillin + H2O + O2
vanillic acid + H2O2
show the reaction diagram
-
oxidation of vanillin is more sensitive to inhibition by flavonoids than that of phenanthridine
-
-
?
vanillin + H2O + O2
vanillic acid + H2O2
show the reaction diagram
S5FPI8
low AtraAOX2 activity
-
-
?
vanillin + O2 + H2O
vanillic acid + H2O2
show the reaction diagram
Pseudomonas sp., Pseudomonas sp. KY 469, Pseudomonas sp. KY 4690
-
-
-
-
?
veratraldehyde + H2O + O2
veratric acid + H2O2
show the reaction diagram
-
18% of activity with n-hexylaldehyde
-
-
?
veratraldehyde + H2O + O2
veratranoate + H2O2
show the reaction diagram
-
120% of activity with formaldehyde
-
-
?
veratraldehyde + H2O + O2
veratranoate + H2O2
show the reaction diagram
-
220% of activity with formaldehyde
-
-
?
veratraldehyde + H2O + O2
veratranoate + H2O2
show the reaction diagram
-
610% of activity with formaldehyde
-
-
?
xanthine + H2O + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
xanthine + H2O + O2
? + H2O2
show the reaction diagram
S5FPI8
lowest AtraAOX2 activity
-
-
?
xanthine + O2 + H2O
urate + H2O2
show the reaction diagram
-
low activity
-
?
XK-469 + H2O + O2
?
show the reaction diagram
-
-
-
-
?
XK-469 + H2O + O2
3-oxo-XK-469 + ?
show the reaction diagram
-
hepatic clearance less than 4.3 ml/(min * kg)
product identification by high-resolution mass spectrometry
-
?
Z11Z13-16Ald + H2O + O2
? + H2O2
show the reaction diagram
S5FPI8
low AtraAOX2 activity
-
-
?
zaleplon + H2O + O2
?
show the reaction diagram
-
-
-
-
?
zaleplon + H2O + O2
5-oxozaleplon + ?
show the reaction diagram
-
hepatic clearance less than 4.3 ml/(min * kg)
product identification by high-resolution mass spectrometry
-
?
zebularine + H2O + O2
uridine + H2O2
show the reaction diagram
-
-
major catabolic route for oral antitumor agent zebularine
-
?
zoniporide + H2O + O2
2-oxozoniporide + H2O2
show the reaction diagram
-
i.e. 1-(quinolin-5-yl)-5-cyclopropyl-1H-pyrazole-4-carbonyl guanidine
-
-
?
zoniporide + H2O + O2
?
show the reaction diagram
-
-
-
-
?
zonisamide + H2O + O2
?
show the reaction diagram
-
-
-
-
?
methotrexate + H2O + O2
7-hydroxymethotrexate + H2O2
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
3(4,5-dimethylthiazolyl-2)2,5-diphenyltetrazolium-bromide, ferricyanide and cytochrome c can also act as electron acceptors, the latter two are not so efficient
-
-
-
additional information
?
-
-
electron acceptors are molecular oxygen, several dyes, ferricyanide, silicomolybdate and organic nitro compounds
-
-
-
additional information
?
-
-
various imidazole, pyrazole, pyridine, pyrazine, pyrimidine, purine, quinoline and pteridine derivatives are substrates for this enzyme, preferences of substitutions are discussed
-
-
-
additional information
?
-
-
various unsubstituted aglycones and some ribonucleosides of various purine analogs are also substrates for this enzyme
-
-
-
additional information
?
-
-
also electron acceptors are dibenzyl sulfoxide, phenothiazine sulfoxide, D-biotin methyl ester d-sulfoxide and quinoline N-oxide
-
-
-
additional information
?
-
O54754
enzyme plays an important role in the biotransformation of drugs and xenobiotics, e.g. antiviral, antimalarial and antitumour compounds and nicotine
-
-
-
additional information
?
-
-
enzyme plays an important role in the biotransformation of drugs and xenobiotics, e.g. antiviral, antimalarial and antitumour compounds and nicotine
-
-
-
additional information
?
-
-
enzyme plays an important role in the biotransformation of drugs and xenobiotics, e.g. antiviral, antimalarial and antitumour compounds and nicotine
-
-
-
additional information
?
-
Q9Z0U5
enzyme plays an important role in the biotransformation of drugs and xenobiotics, e.g. antiviral, antimalarial and antitumour compounds and nicotine
-
-
-
additional information
?
-
P48034
enzyme plays an important role in the biotransformation of drugs and xenobiotics, e.g. antiviral, antimalarial and antitumour compounds and nicotine
-
-
-
additional information
?
-
-
enzyme plays an important role in the biotransformation of drugs and xenobiotics, e.g. antiviral, antimalarial and antitumour compounds and nicotine
-
-
-
additional information
?
-
-
enzyme plays an important role in detoxification of the toxic heterocycles synthesized by certain plants
-
-
-
additional information
?
-
Q9Z0U5
enzyme is of interest as a source of the reactive oxygen species, hydrogen peroxide and the superoxide anion
-
-
-
additional information
?
-
-
catalyzes the last step of indole-3-acetic acid biosynthesis. Local synthesis of indole-3-acetic acid in the root nodule meristem and modulation of enzyme expression and activity may be involved in regulation of nodule development
-
-
-
additional information
?
-
Q4VGM3
enzyme may be involved in degradation of aldehyde odorant compounds such as pheromones or plants volatiles
-
-
-
additional information
?
-
-
enzyme may be involved in synthesis of reactive oxygen species during water stress
-
-
-
additional information
?
-
-
a wide variety of xenobiotics may serve as electron acceptors instead of molecular oxygen. Enzyme reduces nitroguanidines to both nitroso- and aminoguanidines, while nitromethylens are reduced only to the corrsponding nitroso metabolites. Reduction of nitroguanidines to amino metabolites depends on enzyme concentration
-
-
-
additional information
?
-
Q2QB49, Q2QB50
enzyme is devoid of xanthine oxidase or hypoxanthine oxidase activity
-
-
-
additional information
?
-
-
enzyme produces H2O2, but not O2, and requires a Moco sulfurase domain
-
-
-
additional information
?
-
-
no activities toward N1-methylnicotinamide, quinoline, xanthine, methanol, and ethanol
-
-
-
additional information
?
-
-
no substrate: heptaldehyde
-
-
-
additional information
?
-
-
no substrate: xanthine, allopurinol, isoquinoline
-
-
-
additional information
?
-
-
no substrate: zebularine
-
-
-
additional information
?
-
-
the enzyme catalyzes the last step of indole-3-acetic acid biosynthesis in plants, in the root nodules. Local synthesis of indole-3-acetic acid in the root nodule meristem and modulation of aldehyde oxidase expression and activity are involved in regulation of nodule development
-
-
-
additional information
?
-
Q5QE78, Q5QE79, Q5QE80, Q9Z0U5
low and negligible quinine-oxidizing activity
-
-
-
additional information
?
-
Q2QB47, Q2QB48
low and negligible quinine-oxidizing activity
-
-
-
additional information
?
-
synthetic construct
-
oxidation of substrates by aldehyde oxidase appears to be due primarily to the electronic effects of the substituents
-
-
-
additional information
?
-
O54754, Q5SGK3, Q6V956, Q8VJ15
pyridoxal is not recognized by mouse AOH2
-
-
-
additional information
?
-
-
no substrate: 6-nitroquinazoinone
-
-
-
additional information
?
-
-
no substrate: citral, hexanal
-
-
-
additional information
?
-
-
no substrate: xanthine, hypoxanthine
-
-
-
additional information
?
-
B0LAZ9, B0LB00, B0LB01
isoforms AO-alpha and AO-beta cannot oxidize abscisic aldehyde
-
-
-
additional information
?
-
-
phenanthridine N-oxide is not metabolized by the enzyme
-
-
-
additional information
?
-
S5FPI8
AtraAOX2 shows strong activity on aldehyde substrates derived from plants, but weak activity on Z11Z1316Ald, AtraAOX2 does not degrade the carbamate insecticide aldicarb
-
-
?
additional information
?
-
-
hepatic clearance for substrates of aldehyde oxidase i.e. BIBX1382, carbazeran, O6-benzylguanine, zaleplon, and XK-469 is investigated using cryopreserved hepatocytes
confirmation of aldehyde oxidase mediated metabolism via incubations in the presence of water containing the 18-O isotope and corresponding isotope patterns in mass spectra
-
?
additional information
?
-
Streptomyces rimosus ATCC10970
-
no activities toward N1-methylnicotinamide, quinoline, xanthine, methanol, and ethanol
-
-
-
additional information
?
-
Desulfovibrio aminophilus DSM12254
-
no substrate: xanthine, allopurinol, isoquinoline
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
13-cis-retinal + O2 + H2O
13-cis-retinoate + H2O2
show the reaction diagram
-
-
-
?
3 brimonidine + 4 H2O + 4 O2
2-oxobrimonidine + 3-oxobrimonidine + 2,3-dioxobrimonidine + 4 H2O2
show the reaction diagram
-
-
-
-
?
4-hydroxypyrazolo(3,4-d)pyrimidine + O2 + H2O
4,6-dihydroxypyrazolo(3,4-d)pyrimidine + H2O2
show the reaction diagram
-
12-13% of the activity compared to purine, i.e. allopurinol
-
?
9-cis-retinal + O2 + H2O
9-cis-retinoate + H2O2
show the reaction diagram
-
-
-
?
abscisic aldehyde + H2O + O2
abscisic acid + H2O2
show the reaction diagram
-
four aldehyde oxidases are known that have varying affinities to abscisic aldehyde. AOdelta, endcoded by AAO3 specifically catalyses this step in rosette leaves
-
-
?
all-trans-retinal + O2 + H2O
all-trans-retinoate + H2O2
show the reaction diagram
-
-
-
?
all-trans-retinal + O2 + H2O
all-trans-retinoate + H2O2
show the reaction diagram
-
-
-
?
all-trans-retinal + O2 + H2O
all-trans-retinoate + H2O2
show the reaction diagram
-
-
-
?
purine + O2 + H2O
? + H2O2
show the reaction diagram
-
best substrate tested
-
?
retinal + O2 + H2O
retinoate + H2O2
show the reaction diagram
-
-
-
?
retinal + O2 + H2O
retinoate + H2O2
show the reaction diagram
-
-
-
?
retinal + O2 + H2O
retinoate + H2O2
show the reaction diagram
-
-
-
?
retinal + O2 + H2O
retinoate + H2O2
show the reaction diagram
-
involved in vitamin A metabolism, increased activity of cells in hyperthyroidism, decreased activity of cells in hypothyroidism
-
?
retinal + O2 + H2O
retinoate + H2O2
show the reaction diagram
-
activity is dependent on vitamin A levels of tissues
-
?
xanthine + O2 + H2O
urate + H2O2
show the reaction diagram
-
low activity
-
?
hypoxanthine + O2 + H2O
xanthine + H2O2
show the reaction diagram
-
22-26% of the activity compared to purine
-
?
additional information
?
-
O54754
enzyme plays an important role in the biotransformation of drugs and xenobiotics, e.g. antiviral, antimalarial and antitumour compounds and nicotine
-
-
-
additional information
?
-
-
enzyme plays an important role in the biotransformation of drugs and xenobiotics, e.g. antiviral, antimalarial and antitumour compounds and nicotine
-
-
-
additional information
?
-
-
enzyme plays an important role in the biotransformation of drugs and xenobiotics, e.g. antiviral, antimalarial and antitumour compounds and nicotine
-
-
-
additional information
?
-
Q9Z0U5
enzyme plays an important role in the biotransformation of drugs and xenobiotics, e.g. antiviral, antimalarial and antitumour compounds and nicotine
-
-
-
additional information
?
-
P48034
enzyme plays an important role in the biotransformation of drugs and xenobiotics, e.g. antiviral, antimalarial and antitumour compounds and nicotine
-
-
-
additional information
?
-
-
enzyme plays an important role in the biotransformation of drugs and xenobiotics, e.g. antiviral, antimalarial and antitumour compounds and nicotine
-
-
-
additional information
?
-
-
enzyme plays an important role in detoxification of the toxic heterocycles synthesized by certain plants
-
-
-
additional information
?
-
Q9Z0U5
enzyme is of interest as a source of the reactive oxygen species, hydrogen peroxide and the superoxide anion
-
-
-
additional information
?
-
-
catalyzes the last step of indole-3-acetic acid biosynthesis. Local synthesis of indole-3-acetic acid in the root nodule meristem and modulation of enzyme expression and activity may be involved in regulation of nodule development
-
-
-
additional information
?
-
Q4VGM3
enzyme may be involved in degradation of aldehyde odorant compounds such as pheromones or plants volatiles
-
-
-
additional information
?
-
-
enzyme may be involved in synthesis of reactive oxygen species during water stress
-
-
-
additional information
?
-
-
the enzyme catalyzes the last step of indole-3-acetic acid biosynthesis in plants, in the root nodules. Local synthesis of indole-3-acetic acid in the root nodule meristem and modulation of aldehyde oxidase expression and activity are involved in regulation of nodule development
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
FAD
-
-
FAD
-
-
FAD
-
2.1 mol per mol native enzyme
FAD
-
0.3-0.5 mol per mol enzyme
FAD
-
2.03 mol per mol enzyme
FAD
-
-
FAD
-
2 mol per mol enzyme
FAD
-
2.0 mol FAD per mol enzyme
FAD
-
-
FAD
-
aldehyde oxidase probably contains 1 mol of FAD per mol of enzyme
FAD
A8TUB4, A8TUC0
;
FAD
B0LAZ9, B0LB00, B0LB01
-
iron-sulfur centre
G3X982
two centers, FeSI and FeSII
molybdenum cofactor
-
i.e. MoCo
molybdenum cofactor
G3X982
-
molybdopterin
-
-
molybdopterin
-
-
molybdopterin
-
-
molybdopterin
-
-
NAD+
-
enzyme is able to oxidize benzaldehyde without NAD+, but its activity increases by 50% when the cofactor is added. Km value 0.0581 mM
[2Fe-2S]-center
-
two distinct ones
molybdopterin cytosine dinucleotide
-
-
additional information
-
no coenzyme Q10
-
additional information
Q4VGM3
sequence lacks a NAD+ binding site
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
CuCl2
-
1 mM, 123% of initial activity
Fe
-
8.24 mol per mol enzyme
Fe2+
-
2 Fe/S iron centres
Fe2+
-
Fe/S center type I and Fe/S center type II
Fe2+
-
2 Fe/S iron centres
Fe2+
-
2 Fe/S iron centres
Fe2+
-
-
Fe2+
-
-
Fe2+
-
-
Fe2+
-
8.5 g per mol native enzyme
Fe2+
-
Fe/S center type I and Fe/S center type II
Fe2+
-
7.92 g per mol enzyme
Fe2+
-
-
Fe2+
-
8 g per mol enzyme
Fe2+
Q4VGM3
N-terminal domain of enzyme contains a conserved domain corresponding to two 2Fe-2S centres
Fe2+
-
contains two 2Fe-2S clusters permonomer, EPR spectroscopy of 2Fe-2S clusters
Fe2+
A8TUB4, A8TUC0
;
Iron
-
aldehyde oxidase probably contains 2 mol of [2Fe-2S] clusters per mol of enzyme
Iron
-
3.3 mol Fe per mol protein
Mo
-
2.01 mol per mol enzyme
Mo5+
-
-
Mo5+
-
-
Mo5+
-
-
Mo5+
-
1 g per mol native enzyme
Mo5+
-
0.66-1.14 g per mol enzyme
Mo5+
-
2 g per mol enzyme
Mo5+
-
contains 0.7 Mo per monomer, EPR spectroscopy of Mo(V) centre
Molybdenum
-
aldehyde oxidase probably contains 1 mol of molybdenum-molybdpterin-cytosine dinucleotide per mol of enzyme
Molybdenum
-
0.34 mol Mo per mol protein
Molybdenum
A8TUB4, A8TUC0
;
Molybdenum
synthetic construct
-
determination of the transition state energy and geometry for the reaction between Mo-ditholene and formamide gives an activation enthalpy of 31.69 kcal/mol and bond lengths for C-O and S-H of 1.64 and 1.55 A, respectively. Enzyme oxidizes substituted quinazolinone substrates via a concerted mechanism
Molybdenum
B0LAZ9, B0LB00, B0LB01
aldehyde oxidase is a molybdenohydroxylase; aldehyde oxidase is a molybdenohydroxylase; aldehyde oxidase is a molybdenohydroxylase; aldehyde oxidase is a molybdenohydroxylase
additional information
Q9NCL9
cDNA encodes a protein with 2 predicted iron-sulfur centres, there is a highly conserved molybdopterin cofactor binding site between residues 706-752
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(+)-catechin
-
-
(-)-epicatechin
-
-
(Z)1,11-hexadecadien-3-one
-
slight inhibition
1-benzyl-3-[2-(methylthio)-3-phenyl-3,4-dihydroquinazolin-6-yl]thiourea
-
comparison with inhibition of xanthine oxidase
2,4-Dinitrophenol
S5FPI8
50 microM, 41.7% residual AtraAOX2 activity
2-(methylthio)-3-phenyl-3,4-dihydroquinazolin-6-amine
-
comparison with inhibition of xanthine oxidase
2-(methylthio)-6-nitro-3-phenyl-3,4-dihydroquinazoline
-
comparison with inhibition of xanthine oxidase
2-Aminobenzaldehyde
-
complete inhibition at 5 mM, partially reversed by retinal
2-Hydroxy-1,4-naphthoquinone
-
specific inhibitor of enzyme in vitro, 50% inhibition of phenanthridine oxidation at 0.0093 mM
2-mercaptoethanol
-
40% inhibition at 5 mM
3,4,5-trimethoxy-N-[2-(methylthio)-3-benzyl-3,4-dihydroquinazolin-6-yl]benzamide
-
comparison with inhibition of xanthine oxidase
3,4,5-trimethoxy-N-[2-(methylthio)-3-phenyl-3,4-dihydroquinazolin-6-yl]benzamide
-
comparison with inhibition of xanthine oxidase
3,4-Dihydroxybenzaldehyde
-
0.01 mM, 50% and 76% inhibition of phenanthridine and N-methylphthalazine oxidation, respectively; 0.1 mM, 87% inhibition of 2-hydroxy-benzaldehyde oxidation
3,4-dimethoxy-2-phenylethylamine
-
0.1 mM and 1 mM, 48% and 100% inhibition of benzaldehyde oxidation, respectively
3,4-dimethoxy-N-[2-(methylthio)-3-benzyl-3,4-dihydroquinazolin-6-yl]benzamide
-
comparison with inhibition of xanthine oxidase
3,4-dimethoxy-N-[2-(methylthio)-3-phenyl-3,4-dihydroquinazolin-6-yl]benzamide
-
comparison with inhibition of xanthine oxidase
3-benzyl-2-(methylthio)-3,4-dihydroquinazolin-6-amine
-
comparison with inhibition of xanthine oxidase
3-benzyl-2-(methylthio)-6-nitro-3,4-dihydroquinazoline
-
comparison with inhibition of xanthine oxidase
3-benzyl-6-nitro-3,4-dihydroquinazoline-2-thiol
-
comparison with inhibition of xanthine oxidase
3-ethyl-2-(methylthio)-6-nitro-3,4-dihydroquinazoline
-
comparison with inhibition of xanthine oxidase
3-ethyl-6-nitro-3,4-dihydroquinazoline-2-thiol
-
comparison with inhibition of xanthine oxidase
3-Hydroxy-4-methoxybenzaldehyde
-
0.1 mM, 87% and 85% inhibition of phenanthridine and N-methylphthalazine oxidation, respectively; 0.1 mM, 87% inhibition of 2-hydroxy-benzaldehyde oxidation
4'-(9-acridinylamino)methanesulfonaniside
-
dose-dependent inhibition, no complete inhibition
4'-(9-acrydinylamino)methanesulfon-m-anisidide
-
competitive inhibitor
4'-(9-acrydinylamino)methanesulfon-o-anisidide
-
-
4-bromo-N-[2-(methylthio)-3-phenyl-3,4-dihydroquinazolin-6-yl]benzenesulfonamide
-
comparison with inhibition of xanthine oxidase
4-hydroxy-3-methoxy-2-phenylethylamine
-
0.1 mM and 1 mM, 21% and 55% inhibition of benzaldehyde oxidation, respectively
4-hydroxymercuribenzoate
S5FPI8
50 microM, 55.3% residual AtraAOX2 activity
4-methoxy-N-[2-(methylthio)-3-benzyl-3,4-dihydroquinazolin-6-yl]benzamide
-
comparison with inhibition of xanthine oxidase
4-methoxy-N-[2-(methylthio)-3-phenyl-3,4-dihydroquinazolin-6-yl]benzamide
-
comparison with inhibition of xanthine oxidase
4-methyl-N-[2-(methylthio)-3-benzyl-3,4-dihydroquinazolin-6-yl]benzamide
-
comparison with inhibition of xanthine oxidase
4-methyl-N-[2-(methylthio)-3-phenyl-3,4-dihydroquinazolin-6-yl]benzamide
-
comparison with inhibition of xanthine oxidase
5-benzylacyclouridine
-
50% inhibition at about 0.25 mM
5-benzylacyclouridine
-
50% inhibition at about 0.8 mM
5-benzylacyclouridine
-
50% inhibition at about 0.3 mM
6-nitro-3-phenyl-3,4-dihydroquinazoline-2-thiol
-
comparison with inhibition of xanthine oxidase
9-aminoacridine
-
competitive inhibitor
acetaldehyde
-
77% inhibition at 5 mM, partially reversed by retinal
adiponectin
Q5QE78, Q5QE79, Q5QE80, Q9Z0U5
downregulates AOX1 expression by activating peroxisome proliferator-activated receptor-alpha
-
allopurinol
-
45% inhibition at 0.25 mM
allopurinol
-
at 0.01 mM, 13% inhibition of 0.1 mM substrate benzaldehyde, 6% inhibition of 0.05 mM substrate phenanthridine, 7% inhibition of 0.05 mM substrate vanillin
allopurinol
-
-
amidol
-
complete inhibition of sulfoxide reductase and aldehyde oxidase activity at 0.2 mM
amitriptyline
-
0.00026 mM, 50% inhibition
amlodipine
-
0.0055 mM, 50% inhibition
amodiaquine
-
0.00074 mM, 50% inhibition
Amytal
-
48% inhibition at 0.2 mM
Amytal
-
54% inhibition at 1 mM
antimycin A
S5FPI8
10 microM, 45.6% residual AtraAOX2 activity
arsenite
-
65% inhibition at 20 mM
arsenite
-
82-95% inhibition of sulfoxide reductase and aldehyde oxidase activity at 0.1 mM
arsenite
-
-
aspartate
O54754, Q5SGK3, Q6V956, Q8VJ15
neonatal pretreatment, which reduces circulating growth hormone levels, decreases male aldehyde oxidase activity to female levels; neonatal pretreatment, which reduces circulating growth hormone levels, decreases male aldehyde oxidase activity to female levels; neonatal pretreatment, which reduces circulating growth hormone levels, decreases male aldehyde oxidase activity to female levels; neonatal pretreatment, which reduces circulating growth hormone levels, decreases male aldehyde oxidase activity to female levels; neonatal pretreatment, which reduces circulating growth hormone levels, decreases male aldehyde oxidase activity to female levels
azide
-
slight inhibition
benzamidine
-
75% inhibition at 0.1 mM
benzamidine
-
1 mM, 58% inhibition
benzamidine
G3X982
-
beta-carboline
-
-
beta-carboline
O54754, Q5SGK3, Q6V956, Q8VJ15
a far better inhibitor of mouse AOH1 than AOX1; a far better inhibitor of mouse AOH1 than AOX1
beta-ecdysone
S5FPI8
50 microM, 30.1% residual AtraAOX2 activity
-
beta-estradiol
-
51% inhibition at 0.004 mM
beta-estradiol
-
90% inhibition at 0.02 mM
beta-estradiol
-
-
beta-estradiol
S5FPI8
50 microM, 60.2% residual AtraAOX2 activity
catechin
-
-
catechol
-
0.1 mM and 1 mM, 64% and 98% inhibition of benzaldehyde oxidation, respectively
chlorpromazine
-
94% inhibition at 0.1 mM
chlorpromazine
-
80% inhibition of sulfoxide reductase and aldehyde oxidase activity at 0.2 mM
chlorpromazine
-
0.00057 mM, 50% inhibition
chlorpromazine
-
0.5 mM, 48% inhibition of reaction without added electron donor
chlorpromazine
-
0.1 mM, about 95% inhibition
chlorpromazine
-
-
chlorpromazine
-
-
cimetidine
-
0.5 mM, 50% inhibition of reaction without added electron donor
clomipramine
-
0.00048 mM, 50% inhibition
clozapine
-
0.0044 mM, 50% inhibition
clozapine
-
-
Cu2+
-
1 mM, 95% inhibition
CuSO4
-
85% inhibition at 0.1 mM
cyclobenzaprine
-
0.0031 mM, 50% inhibition
daidzein
-
-
dicoumarol
-
-
dicoumarol
-
43% inhibition at 0.1 mM
dicoumarol
-
33% inhibition of sulfoxide reductase and aldehyde oxidase activity at 0.01 mM
Digitonin
S5FPI8
125 microM, 69.9% residual AtraAOX2 activity
diphenylsulfoxide
-
85% inhibition at 0.1 mM, it serves as an inhibitor in presence of other electron acceptors
dithiothreitol
-
45% and 92% inhibition at 0.1 mM and 1 mM respectively
domperidone
-
0.0030 mM, 50% inhibition
domperidone
-
-
dopamine
-
0.1 mM, 80% and 93% inhibition of phenanthridine and N-methylphthalazine oxidation, respectively; 0.1 mM and 1 mM, 59% and 100% inhibition of benzaldehyde oxidation, respectively
DTT
-
more than 50% inhibition at 1.6 mM
epicatechin
-
-
erythromycin
-
0.015 mM, 50% inhibition
estradiol
-
0.00029 mM, 50% inhibition
estradiol
-
-
Estrogen
O54754, Q5SGK3, Q6V956, Q8VJ15
reduces liver aldehyde oxidase activity of male animals; reduces liver aldehyde oxidase activity of male animals; reduces liver aldehyde oxidase activity of male animals; reduces liver aldehyde oxidase activity of male animals; reduces liver aldehyde oxidase activity of male animals
ethanol
-
0.75 M, 40% inhibition
ethanol
S5FPI8
2% (v/v), 62.1% residual AtraAOX2 activity
ethinyl estradiol
-
0.00057 mM, 50% inhibition
ethinyl estradiol
-
-
felodipine
-
0.00030 mM, 50% inhibition
fenofibrate
-
in mature adipocytes, enzyme expression is reduced in presence of 50 microM fenofibrate
genistein
-
-
glutamate
O54754, Q5SGK3, Q6V956, Q8VJ15
neonatal pretreatment, which reduces circulating growth hormone levels, decreases male aldehyde oxidase activity to female levels; neonatal pretreatment, which reduces circulating growth hormone levels, decreases male aldehyde oxidase activity to female levels; neonatal pretreatment, which reduces circulating growth hormone levels, decreases male aldehyde oxidase activity to female levels; neonatal pretreatment, which reduces circulating growth hormone levels, decreases male aldehyde oxidase activity to female levels; neonatal pretreatment, which reduces circulating growth hormone levels, decreases male aldehyde oxidase activity to female levels
hesperetin
-
-
hesperetin
-
-
hesperidin
-
-
hydralazine
-
upon coincubation of BIBX1382, carbazeran, and O6-benzylguanine with 50 microM hydralazine, metabolic clearance is substantially attenuated
hydralazine
-
100 microM reduces enzyme activity by as much as 96%
hydrazine
-
1 mM, 28% residual activity
hydroxyamine
-
1 mM, 52% residual activity
hydroxytoluene
-
butylated, 50% inhibition at 1 mM
hyperoside
-
-
iodoacetate
-
31% inhibition at 1 mM
Isopropanol
-
moderate inhibitor
Isopropanol
S5FPI8
2% (v/v), 67.9% residual AtraAOX2 activity
isovanillin
-
1 mM, approx. 96% inhibition after 120 min
isovanillin
-
-
KCN
-
95% inhibition at 1 mM
KCN
-
46% inhibition at 0.2 mM
KCN
-
59% inhibition at 0.2 mM
KCN
-
complete inhibition at 1 mM when preincubated for 5 min
KCN
-
95% inhibition of sulfoxide reductase and aldehyde oxidase activity at 0.25 mM
KCN
-
1 mM, 18% inhibition of reaction without added electron donor
ketoconazole
-
0.0035 mM, 50% inhibition
L-Dopa
-
0.1 mM and 1 mM, 39% and 94% inhibition of benzaldehyde oxidation, respectively
loperamide
-
0.010 mM, 50% inhibition
loratidine
-
0.00049 mM, 50% inhibition
loxapine
-
0.0023 mM, 50% inhibition
maprotiline
-
0.0014 mM, 50% inhibition
menadione
-
91.5% inhibition at 0.01 mM
menadione
-
slight inhibition at 0.005 mM
menadione
-
complete inhibition at 0.05 mM
menadione
-
-
menadione
-
complete inhibition of tropylium ion oxidation at 0.003 mM
menadione
-
95% inhibition at 0.02 mM
menadione
-
59% inhibition at 0.2 mM
menadione
-
complete inhibition at 0.1 mM
menadione
-
0.006 mM, 0.0042 mM, 0.0026 mM, 0.001 mM, 0.0014 mM and 0.0008 mM, 50% inhibition of N-[2-(dimethylamino)ethyl]acridine-4-carboxamide oxidation in male swiss CD mouse, CB57BI/6J, female swiss CD mouse, nude mouse, nude mouse tumor bearing and C129/C57 muse, respectively
menadione
-
0.005 mM, 74% inhibition; 0.005 mM, 87% inhibition
menadione
-
0.00020 mM, 50% inhibition
menadione
-
at 0.01 mM, 80% inhibition of 0.1 mM substrate benzaldehyde, 96% inhibition of 0.05 mM substrate phenanthridine, 89% inhibition of 0.05 mM substrate vanillin
menadione
-
0.2 mM, 78% inhibition of reaction without added electron donor
menadione
-
0.1 mM, about 95% inhibition
menadione
-
-
menadione
-
inhibitory versus substrates vanillin and phenanthridine, activating versus substrate xanthine
menadione
-
specific inhibitor of aldehyde oxidase
menadione
G3X982
-
menadione
S5FPI8
100 microM, 63.1% residual AtraAOX2 activity
menadione
-
standard aldehyde oxidase inhibitor
methadone
Q9NCL9
0.1 mM, 68% inhibition
methanol
-
0.75 M, 62% inhibition
methanol
S5FPI8
2% (v/v), 33.9% residual AtraAOX2 activity
metoclopramide
-
0.031 mM, 50% inhibition
myricetin
-
inhibition of both aldehyde oxidase and xanthine oxidase
myricetin
-
-
N-hydroxy-2-acetylamino-biphenyl
-
15%, 51% and 88% inhibition at 0.001 mM, 0.01 mM and 0.1 mM respectively
N-hydroxy-2-acetylamino-fluorene
-
38% and 88% inhibition at 0.001 mM and 0.01 mM respectively, protection against inhibition in the presence of dithiothreitol, cysteine and glutathione, noncompetitive inhibitor
N-hydroxy-2-propionylamino-fluorene
-
25% and 52% inhibition at 0.001 mM and 0.01 mM respectively
N-hydroxyphenacetin
-
4%, 27% and 44% inhibition at 0.001 mM, 0.01 mM and 0.1 mM respectively
N-methylnicotinamide
-
competitive inhibition
N-[(1E)-(3,4,5-trimethoxyphenyl)methylene]-2-(methylthio)-3-benzyl-3,4-dihydroquinazolin-6-amine
-
comparison with inhibition of xanthine oxidase
N-[(1E)-(3,4,5-trimethoxyphenyl)methylene]-2-(methylthio)-3-phenyl-3,4-dihydroquinazolin-6-amine
-
comparison with inhibition of xanthine oxidase
N-[(1E)-(3,4-dimethoxyphenyl)methylene]-2-(methylthio)-3-benzyl-3,4-dihydroquinazolin-6-amine
-
comparison with inhibition of xanthine oxidase
N-[(1E)-(3,4-dimethoxyphenyl)methylene]-2-(methylthio)-3-phenyl-3,4-dihydroquinazolin-6-amine
-
comparison with inhibition of xanthine oxidase
N-[(1E)-(4-methoxyphenyl)methylene]-2-(methylthio)-3-benzyl-3,4-dihydroquinazolin-6-amine
-
comparison with inhibition of xanthine oxidase
N-[(1E)-(4-methoxyphenyl)methylene]-2-(methylthio)-3-phenyl-3,4-dihydroquinazolin-6-amine
-
comparison with inhibition of xanthine oxidase
N-[2-(methylthio)-3-phenyl-3,4-dihydroquinazolin-6-yl]benzamide
-
comparison with inhibition of xanthine oxidase
Naringenin
-
-
Naringenin
-
-
nitrate
-
moderate inhibitor
noradrenaline
-
0.1 mM, 51% and 95% inhibition of phenanthridine and N-methylphthalazine oxidation, respectively; 0.1 mM and 1 mM, 90% and 100% inhibition of benzaldehyde oxidation, respectively
norclomipramine
-
0.00060 mM, 50% inhibition
norharman
-
slight inhibition at 0.02 mM
norharman
-
50% inhibition at 0.01 mM
norharmane
-
0.02 mM, 87% inhibition
norharmane
G3X982
-
nortriptyline
-
0.00085 mM, 50% inhibition
olanzapine
-
0.0060 mM, 50% inhibition
ondansetron
-
0.0021 mM, 50% inhibition
p-benzoquinone
-
strong inhibitor
p-chloromercuribenzoate
-
1 mM, 88% inhibition
p-chloromercuribenzoate
-
1 mM, 94% inhibition
p-chloromercuribenzoate
-
1 mM, 90% inhibition
p-chloromercuribenzoic acid
-
-
p-chloromercuribenzoic acid
-
96% inhibition at 0.1 mM
p-chloromercuribenzoic acid
-
61% inhibition at 0.05 mM
p-chloromercuribenzoic acid
-
95% inhibition of sulfoxide reductase and aldehyde oxidase activity at 0.1 mM
p-dimethylaminocinnamaldehyde
-
effective inhibitor, more pronounced at higher pH values
p-hydroxymercuribenzoate
-
complete inhibition at 3 mM, reversed by DTT
palmitic acid
-
enzyme expression is reduced in 3T3-L1 cells differentiated in presence of 400 microM palmitic acid
perphenazine
-
0.00003 mM, 50% inhibition
phenylhydrazine
-
1 mM, 12% residual activity
phosphate
-
moderate inhibitor
potassium cyanide
S5FPI8
1 mM, 71.8% residual AtraAOX2 activity
promazine
-
0.0016 mM, 50% inhibition
Promethazine
-
0.00051 mM, 50% inhibition
Promethazine
-
0.5 mM, 28% inhibition of reaction without added electron donor
propafenone
-
0.0025 mM, 50% inhibition
propyl 3,4,5-trihydroxybenzoate
-
91% inhibition at 3 mM
Pyridine
P80456
non-linear inhibition
quercetin
-
at 0.01 mM, 96% inhibition of 0.1 mM substrate benzaldehyde, 69% inhibition of 0.05 mM substrate phenanthridine, 91% inhibition of 0.05 mM substrate vanillin. Mixed type inhibition for all three substrates
quercetin
-
inhibition of both aldehyde oxidase and xanthine oxidase
quercetin
-
-
quetiapine
-
0.0014 mM, 50% inhibition
Quinacrine
-
74% inhibition at 0.1 mM
Quinacrine
-
45% inhibition at 0.002 mM
Quinacrine
-
76% inhibition at 1 mM, partially reversed by FAD
Quinacrine
-
0.0033 mM, 50% inhibition
Quinacrine
S5FPI8
1 mM, 34.9% residual AtraAOX2 activity
quinocrine
-
59-63% inhibition of sulfoxide reductase and aldehyde oxidase activity at 0.1 mM
quinolinic acid
-
competitive
raloxifene
-
0.000003 mM, 50% inhibition
raloxifene
-
50% inhibition at about 8 nM
raloxifene
-
50% inhibition at about 500 nM
raloxifene
-
50% inhibition above 1100 nM
raloxifene
-
aldehyde oxidase inhibitor, significantly decreases NO generation from nitrite in heart or liver
raloxifene
-
specific inhibitor of aldehyde oxidase, complete inhibition at 0.01 mM
raloxifene
-
inhibits substrate binding at the molybdenum site of the enzyme
raloxifene
-
-
raloxifene
G3X982
-
retinol
-
complete inhibition at 0.5 mM
rotenone
S5FPI8
10 microM, 89.3% residual AtraAOX2 activity
rutin
-
at 0.01 mM, 52% inhibition of 0.1 mM substrate benzaldehyde, 27% inhibition of 0.05 mM substrate phenanthridine, 34% inhibition of 0.05 mM substrate vanillin. Mixed type inhibition for benzaldehyde and vanillin, non-competitive with phenanthridine
salmeterol
-
0.0099 mM, 50% inhibition
scopoletin
-
moderate inhibition, effect on superoxide anion formation is more pronounced than effect on hydrogen peroxide formation or substrate oxidation
selenium
-
enzyme activity towards substrates (S)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine and vanillin is increased by selenium deficiency to 250% of initial rate, and this corresponds to an increase of Aox1 protein level, but not to a decrease in mRNA level
silibinin
-
-
siRNA
Q06278
knock-down of AOX1 in HepG2 cells, significantly reduces ABCA1-dependent lipid efflux and enhances phagocytic uptake of microspheres similar to ABCA1 deficiency, without affecting ABCA1 mRNA and protein levels
-
SKF 525-A
-
60% inhibition at 0.05 mM
SKF 525-A
-
62% inhibition at 0.1 mM
Sodium azide
S5FPI8
5 mM, 52.4% residual AtraAOX2 activity
superoxid dismutase
-
complete inhibition at 0.001 mM
-
tacrine
-
0.0050 mM, 50% inhibition
tamoxifen
-
0.0022 mM, 50% inhibition
tamoxifen
-
-
taxifolin
-
-
thioridazine
-
0.00016 mM, 50% inhibition
Trifluperazine
-
0.00024 mM, 50% inhibition
Triton X-100
-
76% inhibition at 0.0008%
Triton X-100
-
28% and 63% inhibition at 0.007% with O2 and diphenylsulfoxide as electron acceptor respectively
Triton X-100
S5FPI8
0.01% (v/v), 71.8% residual AtraAOX2 activity
tungstate
-
-
verapamil
-
0.0035 mM, 50% inhibition
MgCl2
-
1 mM, 62% residual activity
additional information
-
some purine and pyrazolo[3,4-d]pyrimidines as well as various unsustituted aglycones and some ribonucleosides of various purine analogs are also inhibitors of this enzyme
-
additional information
-
not inhibitory: diphenyleneiodonium
-
additional information
O54754, Q5SGK3, Q6V956, Q8VJ15
activity is significantly reduced by castration of adult males. Hypophysectomy markedly decreases hepatic activity in male and to a lesser extent in female mice; activity is significantly reduced by castration of adult males. Hypophysectomy markedly decreases hepatic activity in male and to a lesser extent in female mice; activity is significantly reduced by castration of adult males. Hypophysectomy markedly decreases hepatic activity in male and to a lesser extent in female mice; activity is significantly reduced by castration of adult males. Hypophysectomy markedly decreases hepatic activity in male and to a lesser extent in female mice; activity is significantly reduced by castration of adult males. Hypophysectomy markedly decreases hepatic activity in male and to a lesser extent in female mice
-
additional information
-
under alkaline conditions, prominent inhibition of NO generation by aldehyde oxidase occurs
-
additional information
-
low aldehyde oxidase activity groups are the Slc:Wistar, Crl:SD, F344/DuCrlCrlj, and Slc:SD strains
-
additional information
-
oxidation of vanillin is more sensitive to inhibition by flavonoids than that of phenanthridine. A rather plan structure is essential for a potent inhibitory effect and substitution by sugar moieties reduces the inhibitory effects
-
additional information
-
addition of 100 microM allopurinol has no significant effect on enzyme activity
-
additional information
S5FPI8
hydroquinone and sodium deoxycholate have no inhibitory effect
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1-hydroxyphthalazine
-
administration to female rabbits causes an increase in the specific activity of liver aldehyde oxidase
dioxin
O54754, Q5SGK3, Q6V956, Q8VJ15
induces AOX1 in mouse hepatoma cells
FAD
-
slight stimulation
menadione
-
inhibitory versus substrates vanillin and phenanthridine, activating versus substrate xanthine
N-methyl-N'-nitro-N-nitrosoguanidine
Q5QE78, Q5QE79, Q5QE80, Q9Z0U5
causes induction of liver aldehyde oxidase; causes induction of liver aldehyde oxidase; causes induction of liver aldehyde oxidase; causes induction of liver aldehyde oxidase
N-methyl-N-nitrosourea
Q5QE78, Q5QE79, Q5QE80, Q9Z0U5
causes induction of liver aldehyde oxidase; causes induction of liver aldehyde oxidase; causes induction of liver aldehyde oxidase; causes induction of liver aldehyde oxidase
NAD+
-
slight stimulation
NAD+
-
stimulation of retinoic acid formation up to 0.1 mM, more effective than NADH
NAD+
-
132% activation
NADH
-
stimulation of retinoic acid formation up to 0.1 mM
NADH
-
79% activation
NADP+
-
95% activation
NADPH
-
179% activation
phenethyl isothiocyanate
O54754, Q5SGK3, Q6V956, Q8VJ15
induces AOX1 transcript through a transcriptional mechanism
Phthalazine
-
administration to female rabbits causes an increase in the specific activity of liver aldehyde oxidase
Sodium azide
-
1 mM, 128% of initial activity
testosterone
O54754, Q5SGK3, Q6V956, Q8VJ15
significantly increases activity in castrated males and normal female mice; significantly increases activity in castrated males and normal female mice; significantly increases activity in castrated males and normal female mice; significantly increases activity in castrated males and normal female mice; significantly increases activity in castrated males and normal female mice
methyl methanesulfonate
Q5QE78, Q5QE79, Q5QE80, Q9Z0U5
causes induction of liver aldehyde oxidase; causes induction of liver aldehyde oxidase; causes induction of liver aldehyde oxidase; causes induction of liver aldehyde oxidase
additional information
-
aldehyde oxidase activity rapidly increases with age up to about one year after birth
-
additional information
Q5QE78, Q5QE79, Q5QE80, Q9Z0U5
fatty liver disease is associated with elevated hepatic AOX1
-
additional information
-
significant correlations between aldehyde oxidase activity and various growth indices like age, body weight, body surface area, and liver volume. Aldehyde oxidase activity rapidly increases with increase of age up to about 1 year. No differences in sex, but individual variation of developmental changes in aldehyde oxidase activity among monozygotic twins
-
additional information
-
under acidic conditions or with mild hypoxia increased NO generation by aldehyde oxidase occurs. NO formation by aldehyde oxidase increases linearly with the increase in nitrite concentration
-
additional information
-
high aldehyde oxidase activity groups are the WKAH/Hkm, WKY/Izm, LEW/CrlCrlj, Crlj:WI, Jcl:Wistar, and Wistar-Imamichi strains
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0109
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine
-
wild type enzyme, in 80 mM phosphate buffer, pH 7.4, at 37C
0.0157
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine
-
mutant enzyme K1004Q/K1005R/M1009I/V1010I/R1021V/A1023Y/I1032V/G1064K/I1067M, in 80 mM phosphate buffer, pH 7.4, at 37C
0.0244
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine
-
mutant enzyme K1004Q/K1005R/M1009I/V1010I/R1021V/A1023Y/I1032V, in 80 mM phosphate buffer, pH 7.4, at 37C
0.0264
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine
-
mutant enzyme A1023Y, in 80 mM phosphate buffer, pH 7.4, at 37C
0.027
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine
-
mutant enzyme M1009I, in 80 mM phosphate buffer, pH 7.4, at 37C
0.0334
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine
-
mutant enzyme I1032V, in 80 mM phosphate buffer, pH 7.4, at 37C
0.036
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine
-
mutant enzyme K1004Q/K1005R, in 80 mM phosphate buffer, pH 7.4, at 37C
0.0366
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine
-
mutant enzyme K1004Q, in 80 mM phosphate buffer, pH 7.4, at 37C
0.0521
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine
-
mutant enzyme K1004Q/K1005R/M1009I/V1010I, in 80 mM phosphate buffer, pH 7.4, at 37C
0.0965
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine
-
mutant enzyme K1004Q/K1005R/M1009I/V1010I/R1021V/A1023Y, in 80 mM phosphate buffer, pH 7.4, at 37C
0.128
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine
-
mutant enzyme A1083T/V1085A, in 80 mM phosphate buffer, pH 7.4, at 37C
0.131
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine
-
mutant enzyme V1085A, in 80 mM phosphate buffer, pH 7.4, at 37C
0.0352
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine
-
mutant enzyme A1081V, in 80 mM phosphate buffer, pH 7.4, at 37C
0.147
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine
-
wild type enzyme, in 80 mM phosphate buffer, pH 7.4, at 37C
0.0341
(S)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine
-
pH 7.4, 37C
0.0407
(S)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine
-
pH 7.4, 37C, enzyme isolated from animals fed with a selenium-deficient diet
0.0027
(S)-RS-8359
-
high affinity component of the expressed enzyme
0.0238
(S)-RS-8359
-
-
0.0754
(S)-RS-8359
-
low affinity component of the expressed enzyme
0.0804
(S)-RS-8359
Q9Z0U5
pH 7.4, 37C, enzyme from Wistar-Imamichi strain, ratio Vmax/Km 1.98 microl per min and mg
0.0813
(S)-RS-8359
Q9Z0U5
pH 7.4, 37C, enzyme from Jcl:Wistar strain, ratio Vmax/Km 1.00 microl per min and mg
0.0833
(S)-RS-8359
Q9Z0U5
pH 7.4, 37C, enzyme from Crj:Wistar strain, ratio Vmax/Km 1.46 microl per min and mg
0.1046
(S)-RS-8359
Q9Z0U5
pH 7.4, 37C, enzyme from LEW:Crj strain, ratio Vmax/Km 0.58 microl per min and mg
0.14
(S)-RS-8359
Q9Z0U5
pH 7.4, 37C, enzyme from F344/DuCrj strain, ratio Vmax/Km 0.01 microl per min and mg
0.17
(S)-RS-8359
Q9Z0U5
pH 7.4, 37C, enzyme from Slc:SD strain, ratio Vmax/Km 0.09 microl per min and mg
0.173
(S)-RS-8359
Q9Z0U5
pH 7.4, 37C, enzyme from Crj:SD strain, ratio Vmax/Km 0.06 microl per min and mg
0.12
1-methyl-3-acetylpyridine
-
ferricyanide as electron acceptor
0.9
13-cis retinal
-
-
0.007
2,6-dichlorophenol indophenol
-
as electron acceptor under aerobic conditions
0.57
2-ethylbutylaldehyde
-
37C, pH 7.0
2.2
2-ethylbutylaldehyde
-
37C, pH 7.0
3.5
2-ethylbutylaldehyde
-
37C, pH 7.0
0.02
2-Hydroxybenzaldehyde
-
37C, pH 7.0
0.0044
2-hydroxypyrimidine
-
-
0.06
2-hydroxypyrimidine
-
25C, pH 7.4
0.097
2-hydroxypyrimidine
-
apparent Km-value of recombinant mAOX3, pH 7.4, 25C
0.17
2-hydroxypyrimidine
-
25C, pH 7.4
0.173
2-hydroxypyrimidine
-
apparent Km-value of native mAOX3, pH 7.4, 25C
1.65
2-hydroxypyrimidine
-
-
8.3
2-hydroxypyrimidine
-
male enzyme
14.7
2-hydroxypyrimidine
-
female enzyme
0.004
2-Methoxybenzaldehyde
-
37C, pH 7.0
0.019
3,4-dimethoxybenzaldehyde
-
37C, pH 7.0
0.005
3-Hydroxybenzaldehyde
-
37C, pH 7.0
0.029
3-Methoxy-4-hydroxybenzaldehyde
-
37C, pH 7.0
0.02
3-Methoxybenzaldehyde
-
37C, pH 7.0
0.0043
4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine
-
chimeric rat/monkey enzyme, high affinity value, pH 6.0, 37C
0.0164
4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine
-
wild-type, low affinity value, pH 6.0, 37C
0.0171
4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine
-
chimeric rat/monkey enzyme, low affinity value, pH 6.0, 37C
0.0351
4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine
-
wild-type, high affinity value, pH 6.0, 37C
0.0363
4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine
-
chimeric monkey/rat enzyme, pH 6.0, 37C
0.0528
4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine
-
wild-type, pH 6.0, 37C
0.0096
4-(dimethylamino)cinnamaldehyde
-
in the presence of 1 mM nitrite, at pH 6.0 and 37C
0.008
4-hydroxybenzaldehyde
-
37C, pH 7.0
0.027
6-chloroquinazolin-4(3H)-one
-
pH 7.4, 22C
0.04
6-ethyl-5H-dibenz(c,e)azepine
-
2,6-dichlorophenol-indophenol as electron acceptor in D2O
0.06
6-ethyl-5H-dibenz(c,e)azepine
-
2,6-dichlorophenol-indophenol as electron acceptor in H2O
0.08
6-ethyl-5H-dibenz(c,e)azepine
-
O2 as electron acceptor in H2O
0.09
6-ethyl-5H-dibenz(c,e)azepine
-
O2 as electron acceptor in D2O
0.293
6-methoxyquinazolin-4(3H)-one
-
pH 7.4, 22C
0.142
6-methylquinazolin-4(3H)-one
-
pH 7.4, 22C
0.2
6-methylthiopurine
-
O2 as electron acceptor
0.3
6-methylthiopurine
-
2,6-dichlorophenol indophenol as electron acceptor
0.013
9-cis retinal
-
-
0.0025
acetaldehyde
-
30C, pH 7.0
0.13
acetaldehyde
-
37C, pH 7.0
0.25
acetaldehyde
-
-
0.345
acetaldehyde
-
2,6-dichlorophenol-indophenol as electron acceptor
0.5199
acetaldehyde
-
wild-type, 30C, pH 7.4
0.8
acetaldehyde
-
25C, pH 7.4
0.82
acetaldehyde
-
25C, pH 7.4
1.3
acetaldehyde
-
37C, pH 7.0
1.4
acetaldehyde
-
37C, pH 7.0
3.8
acetaldehyde
-
pH 7.0, 30C
52.9
acetaldehyde
-
mutant V806E, 30C, pH 7.4
0.48
acrolein
-
37C, pH 7.0
0.74
acrolein
-
37C, pH 7.0
1.4
acrolein
-
37C, pH 7.0
0.125
all-trans retinal
-
-
0.031
all-trans retinaldehyde
O54754, Q5SGK3, Q6V956, Q8VJ15
-
0.07
all-trans retinaldehyde
O54754, Q5SGK3, Q6V956, Q8VJ15
-
0.008
all-trans-retinal
-
-
0.00057
benzaldehyde
-
30C, pH 7.0
0.0015
benzaldehyde
-
O2 as electron acceptor
0.0023
benzaldehyde
G3X982
K889H mutant, pH 8.0, 37C
0.0025
benzaldehyde
G3X982
mAOX3 wild type, pH 8.0, 37C
0.0029
benzaldehyde
G3X982
A807V mutant, pH 8.0, 37C
0.004 - 0.015
benzaldehyde
-
-
0.004
benzaldehyde
-
25C, pH 7.4
0.0046
benzaldehyde
G3X982
A807V/Y885M double mutant, pH 8.0, 37C
0.005
benzaldehyde
-
2,6-dichlorophenol-indophenol as electron acceptor
0.0063
benzaldehyde
G3X982
Y885M mutant, pH 8.0, 37C
0.0103
benzaldehyde
-
pH 7.8, determined according to Eadie-Hofstee
0.0107
benzaldehyde
-
pH 7.8, determined according to Hanes
0.012
benzaldehyde
-
25C, pH 7.4
0.013
benzaldehyde
-
apparent Km-value of native mAOX3, pH 7.4, 25C
0.0147
benzaldehyde
-
pH 7.8, determined according to Lineweaver-Burk
0.0165
benzaldehyde
-
pH 7.8, determined according to Eadie-Hofstee
0.0178
benzaldehyde
-
pH 7.8, determined according to Lineweaver-Burk
0.019
benzaldehyde
-
-
0.019
benzaldehyde
-
37C, pH 7.0
0.0191
benzaldehyde
-
pH 7.8, determined according to Hanes
0.02
benzaldehyde
-
apparent Km-value of recombinant mAOX3, pH 7.4, 25C
0.0238
benzaldehyde
-
partially purified protein, pH 7.0, 30C
0.039
benzaldehyde
-
female enzyme treated with testosterone propionate
0.04
benzaldehyde
-
male enzyme
0.0863
benzaldehyde
G3X982
E1266Q mutant, pH 8.0, 37C
0.0977
benzaldehyde
-
wild-type, 30C, pH 7.4
0.115
benzaldehyde
-
female enzyme
0.151
benzaldehyde
-
-
0.52
benzaldehyde
-
37C, pH 7.0
0.6345
benzaldehyde
-
mutant V806E, 30C, pH 7.4
2.2
benzaldehyde
-
pH 7.0, 30C
7.13
benzaldehyde
-
mutant M884R, 30C, pH 7.4
0.026
Butanal
-
apparent Km-value of recombinant mAOX3, pH 7.4, 25C
0.029
Butanal
-
apparent Km-value of native mAOX3, pH 7.4, 25C
0.105
butanaldehyde
-
-
0.0005
Butyraldehyde
-
30C, pH 7.0
0.008
Butyraldehyde
-
25C, pH 7.4
0.021
Butyraldehyde
-
25C, pH 7.4
0.026
Butyraldehyde
-
2,6-dichlorophenol-indophenol as electron acceptor
0.0074
cinchonidine
-
wild type enzyme, in 80 mM phosphate buffer, pH 7.4, at 37C
0.012
cinchonidine
-
mutant enzyme V1085A, in 80 mM phosphate buffer, pH 7.4, at 37C
0.1039
cinnamylaldehyde
-
partially purified protein, pH 7.0, 30C
0.06
citral
-
37C, pH 7.0
0.12
citral
-
37C, pH 7.0
2.1
citral
-
37C, pH 7.0
0.052
clothianidin
-
aminoguanidine formation, pH 7.4, 37C
1.03
clothianidin
-
nitrosoguanidine formation, pH 7.4, 37C
0.02
diphenylsulfoxide
-
as electron acceptor under anaerobic conditions
0.31
DL-glyceraldehyde
-
37C, pH 7.0
0.03
ferricyanide
-
as electron acceptor under aerobic conditions
3.6
formaldehyde
-
37C, pH 7.0
4.2
formaldehyde
-
37C, pH 7.0
8.5
formaldehyde
-
37C, pH 7.0
8.8
formaldehyde
-
pH 7.0, 30C
21.2
formaldehyde
-
37C, pH 7.0
29
formaldehyde
-
37C, pH 7.0
0.29
Glutaraldehyde
-
37C, pH 7.0
0.6
Glutaraldehyde
-
pH 7.0, 30C
0.0097
Glyoxal
-
30C, pH 7.0
3.8
Glyoxal
-
pH 7.0, 30C
0.00014
Hexylaldehyde
-
30C, pH 7.0
0.15
Hexylaldehyde
-
37C, pH 7.0
0.26
Hexylaldehyde
-
37C, pH 7.0
1.8
Hexylaldehyde
-
37C, pH 7.0
1.4
hypoxanthine
-
-
0.16
imidacloprid
-
aminoguanidine formation, pH 7.4, 37C
2.99
imidacloprid
-
nitrosoguanidine formation, pH 7.4, 37C
0.0032
Indole-3-acetaldehyde
-
O2 as electron acceptor
0.005
Indole-3-acetaldehyde
-
2,6-dichlorophenol-indophenol as electron acceptor
2.07
Indole-3-acetaldehyde
-
partially purified protein, pH 7.0, 30C
0.0045
Indole-3-aldehyde
-
O2 as electron acceptor
0.014
Indole-3-aldehyde
-
2,6-dichlorophenol-indophenol as electron acceptor
1.8
m-tolualdehyde
-
pH 7.0, 30C
0.73
n-Heptanal
-
pH 7.0, 30C
0.11
n-Heptylaldehyde
-
37C, pH 7.0
0.8
n-Hexanal
-
pH 7.0, 30C
0.22
n-Hexylaldehyde
-
37C, pH 7.0
0.08
N-methylnicotinamide
-
2,6-dichlorophenol-indophenol as electron acceptor in D2O
0.26
N-methylnicotinamide
-
O2 as electron acceptor in D2O
0.32
N-methylnicotinamide
-
2,6-dichlorophenol-indophenol as electron acceptor in H2O
0.355
N-methylnicotinamide
-
female rat liver enzyme after reduction with DTT
0.36
N-methylnicotinamide
-
male rat liver enzyme after reduction with DTT
0.36
N-methylnicotinamide
-
-
0.45
N-methylnicotinamide
-
O2 as electron acceptor in H2O
0.538
N-methylnicotinamide
-
male rat liver enzyme
0.66
N-methylnicotinamide
-
-
1.063
N-methylnicotinamide
-
female rat liver enzyme
1.5
N-methylnicotinamide
-
ferricyanide as electron acceptor
1.895
N-methylnicotinamide
-
female rat liver enzyme after reduction with DTT and reoxidation with 4,4'-dithiodipyridine
7
N-methylnicotinamide
-
-
1.5
n-Pentanal
-
pH 7.0, 30C
0.18
n-Valeraldehyde
-
37C, pH 7.0
0.008 - 0.014
N-[(2-dimethylamino)ethyl] acridine-4-carboxamide
-
-
0.011
N-[(2-dimethylamino)ethyl] acridine-4-carboxamide
-
female enzyme treated with testosterone propionate
0.012
N-[(2-dimethylamino)ethyl] acridine-4-carboxamide
-
male enzyme
0.049
N-[(2-dimethylamino)ethyl] acridine-4-carboxamide
-
female enzyme
0.0063
N-[(2-dimethylamino)ethyl]acridine-4-carboxamide
-
pH 7.4 and 37C
0.0029
N-[2-(Dimethylamino)ethyl]acridine-4-carboxamide
-
37C, pH 7.8, strain c129/C57
0.0055
N-[2-(Dimethylamino)ethyl]acridine-4-carboxamide
-
37C, pH 7.8, nude mouse tumor bearing
0.0074
N-[2-(Dimethylamino)ethyl]acridine-4-carboxamide
-
37C, pH 7.8, nude mouse
0.027
N-[2-(Dimethylamino)ethyl]acridine-4-carboxamide
-
37C, pH 7.8, female swiss CD
0.03
N-[2-(Dimethylamino)ethyl]acridine-4-carboxamide
-
37C, pH 7.8, male swiss CD
0.05
N-[2-(Dimethylamino)ethyl]acridine-4-carboxamide
-
37C, pH 7.8, strain CB57BI/6J
0.0255
N1-methylnicotinamide
G3X982
K889H mutant, pH 8.0, 37C
0.0359
N1-methylnicotinamide
G3X982
A807V/Y885M double mutant, pH 8.0, 37C
0.0827
N1-methylnicotinamide
G3X982
Y885M mutant, pH 8.0, 37C
0.1234
N1-methylnicotinamide
G3X982
A807V mutant, pH 8.0, 37C
0.1285
N1-methylnicotinamide
G3X982
mAOX3 wild type, pH 8.0, 37C
0.024
NADH
-
in the presence of 1 mM nitrite, at pH 6.0 and 37C
0.084
nitenpyram
-
aminoguanidine formation, pH 7.4, 37C
2.41
nitenpyram
-
nitrosoguanidine formation, pH 7.4, 37C
2.7
nitrite
-
in the presence of 0.1 mM NADH, at pH 6.0 and 37C
3.3
nitrite
-
in the presence of 0.05 mM 4-(dimethylamino)cinnamaldehyde, at pH 6.0 and 37C
4.8
o-tolualdehyde
-
pH 7.0, 30C
0.0026
p-Hydroxybenzaldehyde
-
30C, pH 7.0
0.0008
p-Methoxybenzaldehyde
-
30C, pH 7.0
1.4
p-tolualdehyde
-
pH 7.0, 30C
0.005
phenanthridine
P80456
methanol, pH 7.4, at 35C
0.00625
phenanthridine
P80456
pyridine, pH 7.4, at 35C
0.0063
phenanthridine
P80456
acetonitrile, pH 7.4, at 35C
0.00666
phenanthridine
P80456
dioxane, pH 7.4, at 35C
0.00769
phenanthridine
P80456
2-propanol, pH 7.4, at 35C
0.00833
phenanthridine
P80456
1-propanol, pH 7.4, at 35C; ethanol, pH 7.4, at 35C
0.01
phenanthridine
P80456
N-N-dimethylformamide, pH 7.4, at 35C
0.011
phenanthridine
P80456
phosphate buffer, pH 7.4, at 35C; tetrahydrofuran, pH 7.4, at 35C
0.0118
phenanthridine
G3X982
A807V/Y885M double mutant, pH 8.0, 37C
0.0161
phenanthridine
G3X982
K889H mutant, pH 8.0, 37C
0.0323
phenanthridine
G3X982
mAOX3 wild type, pH 8.0, 37C
0.0334
phenanthridine
G3X982
Y885M mutant, pH 8.0, 37C
0.1495
phenanthridine
G3X982
A807V mutant, pH 8.0, 37C
0.25
phenylacetaldehyde
-
2,6-dichlorophenol-indophenol as electron acceptor
0.0011
Phthalazine
G3X982
K889H mutant, pH 8.0, 37C
0.0014
Phthalazine
G3X982
mAOX3 wild type, pH 8.0, 37C
0.0027
Phthalazine
G3X982
A807V mutant, pH 8.0, 37C
0.0029
Phthalazine
G3X982
A807V/Y885M double mutant, pH 8.0, 37C
0.0032
Phthalazine
G3X982
Y885M mutant, pH 8.0, 37C
0.0114
Phthalazine
-
wild-type, 30C, pH 7.4
0.0285
Phthalazine
-
mutant V806E, 30C, pH 7.4
0.0028
propionaldehyde
-
30C, pH 7.0
0.012
propionaldehyde
-
-
0.074
propionaldehyde
-
2,6-dichlorophenol-indophenol as electron acceptor
0.0029
Protocatechualdehyde
-
O2 as electron acceptor
0.026
Protocatechualdehyde
-
2,6-dichlorophenol-indophenol as electron acceptor
0.22
purine
-
-
0.399
quinazolin-4(3H)-one
-
pH 7.4, 22C
0.0038
retinal
-
-
0.008
retinal
-
-
0.163
retinal
-
-
0.25
retinal
-
-
0.03
retinalaldehyde
-
25C, pH 7.4
0.07
retinalaldehyde
-
25C, pH 7.4
0.0075
retinaldehyde
-
mutant M884R, 30C, pH 7.4
0.0222
retinaldehyde
-
mutant V806E, 30C, pH 7.4
0.0558
retinaldehyde
-
wild-type, 30C, pH 7.4
0.07
Salicylaldehyde
-
37C, pH 7.0
0.34
Salicylaldehyde
-
37C, pH 7.0
0.7
Salicylaldehyde
-
37C, pH 7.0
5.2
Salicylaldehyde
-
pH 7.0, 30C
1.15
tropylium tetrafluoroborate
-
ferricyanide as electron acceptor
0.0011
Valeraldehyde
-
30C, pH 7.0
0.014
Vanillin
-
pH 7.4, 37C
0.0158
Vanillin
-
pH 7.4, 37C, enzyme isolated from animals fed with a selenium-deficient diet
1.5
Vanillin
-
37C, pH 7.0
6
Vanillin
-
pH 7.0, 30C
1.7
xanthine
-
-
0.0073
zebularine
-
male, pH 7.5, 37C
0.0084
zebularine
-
female, pH 7.5, 37C
0.011
zebularine
-
female, pH 7.5, 37C; male, pH 7.5, 37C
0.015
zebularine
-
male, pH 7.5, 37C
0.027
zebularine
-
female, pH 7.5, 37C
0.102
zebularine
-
male, pH 7.5, 37C
0.0034
zoniporide
-
in 25 mM phosphate buffer at 37C, pH not specified in the publication
0.002
methylene blue
-
as electron acceptor under aerobic conditions
additional information
additional information
G3X982
further kinetic parameters of phthalazine-DCPIP reaction in the absence or presence of the inhibitors benzamidine, menadione, norharmane, and raloxifene available in the publication
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
6.88
2-hydroxypyrimidine
-
apparent Kcat-value of recombinant mAOX3, pH 7.4, 25C
21.32
2-hydroxypyrimidine
-
apparent Kcat-value of native mAOX3, pH 7.4, 25C
2.7
acetaldehyde
-
mutant V806E, 30C, pH 7.4
8.66
acetaldehyde
-
wild-type, 30C, pH 7.4
0.17
benzaldehyde
G3X982
E1266Q mutant, pH 8.0, 37C
0.32
benzaldehyde
G3X982
K889H mutant, pH 8.0, 37C
0.683
benzaldehyde
G3X982
A807V mutant, pH 8.0, 37C
0.698
benzaldehyde
G3X982
mAOX3 wild type, pH 8.0, 37C
0.727
benzaldehyde
G3X982
A807V/Y885M double mutant, pH 8.0, 37C
0.733
benzaldehyde
-
apparent Kcat-value of recombinant mAOX3, pH 7.4, 25C
0.957
benzaldehyde
G3X982
Y885M mutant, pH 8.0, 37C
1.27
benzaldehyde
-
mutant M884R, 30C, pH 7.4
2.167
benzaldehyde
-
apparent Kcat-value of native mAOX3, pH 7.4, 25C
2.52
benzaldehyde
-
mutant V806E, 30C, pH 7.4
5.29
benzaldehyde
-
wild-type, 30C, pH 7.4
95.2
benzaldehyde
-
partially purified protein, pH 7.0, 30C
2.333
Butanal
-
apparent Kcat-value of recombinant mAOX3, pH 7.4, 25C
6.4
Butanal
-
apparent Kcat-value of native mAOX3, pH 7.4, 25C
436.5
cinnamylaldehyde
-
partially purified protein, pH 7.0, 30C
0.447
N-methylnicotinamide
-
electron acceptor: cytochrome c
1.14
N-methylnicotinamide
-
electron acceptor: nitro blue tetrazolium
1.81
N-methylnicotinamide
-
electron acceptor: dichlorophenolinodphenol
1.97
N-methylnicotinamide
-
electron acceptor: ferricyanide
0.052
N1-methylnicotinamide
G3X982
K889H mutant, pH 8.0, 37C
0.187
N1-methylnicotinamide
G3X982
A807V mutant, pH 8.0, 37C
0.245
N1-methylnicotinamide
G3X982
mAOX3 wild type, pH 8.0, 37C
0.39
N1-methylnicotinamide
G3X982
Y885M mutant, pH 8.0, 37C
0.45
N1-methylnicotinamide
G3X982
A807V/Y885M double mutant, pH 8.0, 37C
0.862
phenanthridine
G3X982
mAOX3 wild type, pH 8.0, 37C
1.33
phenanthridine
G3X982
K889H mutant, pH 8.0, 37C
3.63
phenanthridine
G3X982
A807V/Y885M double mutant, pH 8.0, 37C
4.45
phenanthridine
G3X982
Y885M mutant, pH 8.0, 37C
5.25
phenanthridine
G3X982
A807V mutant, pH 8.0, 37C
0.19
Phthalazine
-
wild-type, 30C, pH 7.4
0.197
Phthalazine
G3X982
K889H mutant, pH 8.0, 37C
0.685
Phthalazine
G3X982
A807V mutant, pH 8.0, 37C; mAOX3 wild type, pH 8.0, 37C
0.69
Phthalazine
G3X982
A807V/Y885M double mutant, pH 8.0, 37C
0.815
Phthalazine
G3X982
Y885M mutant, pH 8.0, 37C
1.72
Phthalazine
-
mutant V806E, 30C, pH 7.4
0.022
retinaldehyde
-
mutant M884R, 30C, pH 7.4
0.23
retinaldehyde
-
mutant V806E, 30C, pH 7.4
0.83
retinaldehyde
-
wild-type, 30C, pH 7.4
1904
Indole-3-acetaldehyde
-
partially purified protein, pH 7.0, 30C
additional information
additional information
G3X982
further kinetic parameters of phthalazine-DCPIP reaction in the absence or presence of the inhibitors benzamidine, menadione, norharmane, and raloxifene available in the publication
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.97
benzaldehyde
G3X982
E1266Q mutant, pH 8.0, 37C
146
136.7
benzaldehyde
G3X982
K889H mutant, pH 8.0, 37C
146
151.8
benzaldehyde
G3X982
Y885M mutant, pH 8.0, 37C
146
158
benzaldehyde
G3X982
A807V/Y885M double mutant, pH 8.0, 37C
146
235.7
benzaldehyde
G3X982
A807V mutant, pH 8.0, 37C
146
279.3
benzaldehyde
G3X982
mAOX3 wild type, pH 8.0, 37C
146
1.52
N1-methylnicotinamide
G3X982
A807V mutant, pH 8.0, 37C
3832
1.92
N1-methylnicotinamide
G3X982
mAOX3 wild type, pH 8.0, 37C
3832
2
N1-methylnicotinamide
G3X982
K889H mutant, pH 8.0, 37C
3832
4.72
N1-methylnicotinamide
G3X982
Y885M mutant, pH 8.0, 37C
3832
12.5
N1-methylnicotinamide
G3X982
A807V/Y885M double mutant, pH 8.0, 37C
3832
26.7
phenanthridine
G3X982
mAOX3 wild type, pH 8.0, 37C
3015
35.2
phenanthridine
G3X982
A807V mutant, pH 8.0, 37C
3015
82.8
phenanthridine
G3X982
K889H mutant, pH 8.0, 37C
3015
133.2
phenanthridine
G3X982
Y885M mutant, pH 8.0, 37C
3015
307.8
phenanthridine
G3X982
A807V/Y885M double mutant, pH 8.0, 37C
3015
175.5
Phthalazine
G3X982
K889H mutant, pH 8.0, 37C
3091
238
Phthalazine
G3X982
A807V/Y885M double mutant, pH 8.0, 37C
3091
253.7
Phthalazine
G3X982
A807V mutant, pH 8.0, 37C
3091
254.7
Phthalazine
G3X982
Y885M mutant, pH 8.0, 37C
3091
489.3
Phthalazine
G3X982
mAOX3 wild type, pH 8.0, 37C
3091
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.53
1-benzyl-3-[2-(methylthio)-3-phenyl-3,4-dihydroquinazolin-6-yl]thiourea
-
37C
0.148
2-(methylthio)-3-phenyl-3,4-dihydroquinazolin-6-amine
-
37C
0.113
2-(methylthio)-6-nitro-3-phenyl-3,4-dihydroquinazoline
-
37C
0.425
3,4,5-trimethoxy-N-[2-(methylthio)-3-benzyl-3,4-dihydroquinazolin-6-yl]benzamide
-
37C
0.43
3,4,5-trimethoxy-N-[2-(methylthio)-3-phenyl-3,4-dihydroquinazolin-6-yl]benzamide
-
37C
0.00016
3,4-Dihydroxybenzaldehyde
-
37C, pH 7.0, inhibition of 2-hydroxybenzaldehyde oxidation
0.51
3,4-dimethoxy-N-[2-(methylthio)-3-benzyl-3,4-dihydroquinazolin-6-yl]benzamide
-
37C
0.55
3,4-dimethoxy-N-[2-(methylthio)-3-phenyl-3,4-dihydroquinazolin-6-yl]benzamide
-
37C
0.136
3-benzyl-2-(methylthio)-3,4-dihydroquinazolin-6-amine
-
37C
0.109
3-benzyl-2-(methylthio)-6-nitro-3,4-dihydroquinazoline
-
37C
0.184
3-benzyl-6-nitro-3,4-dihydroquinazoline-2-thiol
-
37C
0.142
3-ethyl-2-(methylthio)-6-nitro-3,4-dihydroquinazoline
-
37C
0.105
3-ethyl-6-nitro-3,4-dihydroquinazoline-2-thiol
-
37C
0.00066
3-Hydroxy-4-methoxybenzaldehyde
-
37C, pH 7.0, inhibition of 2-hydroxybenzaldehyde oxidation
0.00006
4'-(9-acrydinylamino)methanesulfon-m-anisidide
-
competitive inhibitor
0.0006
4'-(9-acrydinylamino)methanesulfon-o-anisidide
-
competitive inhibitor
0.52
4-bromo-N-[2-(methylthio)-3-phenyl-3,4-dihydroquinazolin-6-yl]benzenesulfonamide
-
37C
0.445
4-methoxy-N-[2-(methylthio)-3-benzyl-3,4-dihydroquinazolin-6-yl]benzamide
-
37C
0.45
4-methoxy-N-[2-(methylthio)-3-phenyl-3,4-dihydroquinazolin-6-yl]benzamide
-
37C
0.46
4-methyl-N-[2-(methylthio)-3-benzyl-3,4-dihydroquinazolin-6-yl]benzamide
-
37C
0.505
4-methyl-N-[2-(methylthio)-3-phenyl-3,4-dihydroquinazolin-6-yl]benzamide
-
37C
0.103
6-nitro-3-phenyl-3,4-dihydroquinazoline-2-thiol
-
37C
0.003
9-aminoacridine
-
competitive inhibitor
0.0225
benzamidine
G3X982
competitive inhibition of phthalazine-2,6-dichlorophenol indophenol reaction, pH 8.0, 37C
0.00087
beta-estradiol
-
Ki-value for free enzyme with DACA as substrate, assay at 100 microM concentration, pH 7.4 and 37C
0.0044
beta-estradiol
-
Ki-value for enzyme-substrate complex with DACA as substrate, assay at 100 microM concentration, pH 7.4 and 37C
0.00062
chlorpromazine
-
Ki-value for free enzyme with DACA as substrate, assay at 100 microM concentration, pH 7.4 and 37C
0.086
chlorpromazine
-
substrate indole-3-aldehyde, pH 7.0, 37C
0.0051
clozapine
-
Ki-value for free enzyme with DACA as substrate, assay at 100 microM concentration, pH 7.4 and 37C
0.0012
domperidone
-
Ki-value for free enzyme with DACA as substrate, assay at 100 microM concentration, pH 7.4 and 37C
0.00043
ethinyl estradiol
-
Ki-value for free enzyme with DACA as substrate, assay at 100 microM concentration, pH 7.4 and 37C
0.0036
ethinyl estradiol
-
Ki-value for enzyme-substrate complex with DACA as substrate, assay at 100 microM concentration, pH 7.4 and 37C
0.0003 - 0.0086
menadione
-
-
0.00047
menadione
-
Ki-value for free enzyme with DACA as substrate, assay at 100 microM concentration, pH 7.4 and 37C
0.00125
menadione
G3X982
uncompetitive inhibition of phthalazine-2,6-dichlorophenol indophenol reaction, pH 8.0, 37C
0.0015
menadione
-
Ki-value for enzyme-substrate complex with DACA as substrate, assay at 100 microM concentration, pH 7.4 and 37C
0.0044
N-hydroxy-2-acetylamino-fluorene
-
noncompetitive inhibitor
2.1
N-methylnicotinamide
-
-
0.4
N-[(1E)-(3,4,5-trimethoxyphenyl)methylene]-2-(methylthio)-3-benzyl-3,4-dihydroquinazolin-6-amine
-
37C
0.435
N-[(1E)-(3,4,5-trimethoxyphenyl)methylene]-2-(methylthio)-3-phenyl-3,4-dihydroquinazolin-6-amine
-
37C
0.37
N-[(1E)-(3,4-dimethoxyphenyl)methylene]-2-(methylthio)-3-benzyl-3,4-dihydroquinazolin-6-amine
-
37C
0.295
N-[(1E)-(3,4-dimethoxyphenyl)methylene]-2-(methylthio)-3-phenyl-3,4-dihydroquinazolin-6-amine
-
37C
0.31
N-[(1E)-(4-methoxyphenyl)methylene]-2-(methylthio)-3-benzyl-3,4-dihydroquinazolin-6-amine
-
37C
0.31
N-[(1E)-(4-methoxyphenyl)methylene]-2-(methylthio)-3-phenyl-3,4-dihydroquinazolin-6-amine
-
37C
0.505
N-[2-(methylthio)-3-phenyl-3,4-dihydroquinazolin-6-yl]benzamide
-
37C
0.0007
norharmane
-
25C, pH 7.4
0.018
norharmane
G3X982
competitive inhibition of phthalazine-2,6-dichlorophenol indophenol reaction, pH 8.0, 37C
0.08
p-dimethylaminocinnamaldehyde
-
pH 9, p-aminobenzamidine oxidation
0.165
p-dimethylaminocinnamaldehyde
-
pH 9, benzaldehyde oxidation
0.575
p-dimethylaminocinnamaldehyde
-
pH 7, p-aminobenzamidine oxidation
1.04
p-dimethylaminocinnamaldehyde
-
pH 7, benzaldehyde oxidation
0.0004
quercetin
-
pH 7.0, 37C, substrate benzaldehyde
0.0005
quercetin
-
pH 7.0, 37C, substrate vanillin
0.0017
quercetin
-
pH 7.0, 37C, substrate phenanthridine
0.086
quinolinic acid
-
substrate phthalazine, pH 7.0, 37C
0.0000023
raloxifene
-
Ki-value for free enzyme with DACA as substrate, assay at 100 microM concentration, pH 7.4 and 37C
0.0122
raloxifene
G3X982
competitive inhibition of phthalazine-2,6-dichlorophenol indophenol reaction, pH 8.0, 37C
0.0591
raloxifene
G3X982
uncompetitive inhibition of phthalazine-2,6-dichlorophenol indophenol reaction, pH 8.0, 37C
0.00092
rutin
-
pH 7.0, 37C, substrate vanillin
0.001
rutin
-
pH 7.0, 37C, substrate benzaldehyde
0.005
menadione
-
-
additional information
additional information
-
table of inhibitor constants for some purine and pyrazolo[3,4-d]pyrimidines as well as of various unsubstituted aglycones and some ribonucleosides of various purine analogs
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0025
(+)-catechin
-
substrate vanillin, pH 7.0, 37C
0.0061
(+)-catechin
-
substrate phenanthridine, pH 7.0, 37C
0.0709
(+)-catechin
-
substrate xanthine, pH 7.0, 37C
0.0057
(-)-epicatechin
-
substrate vanillin, pH 7.0, 37C
0.0061
(-)-epicatechin
-
substrate phenanthridine, pH 7.0, 37C
0.0592
(-)-epicatechin
-
substrate xanthine, pH 7.0, 37C
0.0123
catechin
-
pH 7.0 and 37C
0.02455
daidzein
-
pH 7.0 and 37C
0.0166
epicatechin
-
pH 7.0 and 37C
0.00468
genistein
-
pH 7.0 and 37C
0.0043
hesperetin
-
substrate vanillin, pH 7.0, 37C
0.0098
hesperetin
-
substrate phenanthridine, pH 7.0, 37C
0.02399
hesperetin
-
pH 7.0 and 37C
0.0266
hesperetin
-
substrate xanthine, pH 7.0, 37C
0.02042
hesperidin
-
pH 7.0 and 37C
0.01995
hyperoside
-
pH 7.0 and 37C
0.0004
menadione
-
substrate vanillin, pH 7.0, 37C
0.0019
menadione
-
substrate phenanthridine, pH 7.0, 37C
0.0037
menadione
-
pH 7.0 and 37C
0.0098
menadione
-
in 25 mM phosphate buffer at 37C, pH not specified in the publication
0.0037
morin
-
substrate vanillin, pH 7.0, 37C
0.0069
morin
-
substrate xanthine, pH 7.0, 37C
0.01585
morin
-
pH 7.0 and 37C
0.0301
morin
-
substrate phenanthridine, pH 7.0, 37C
0.0004
myricetin
-
substrate vanillin, pH 7.0, 37C; substrate xanthine, pH 7.0, 37C
0.0021
myricetin
-
substrate phenanthridine, pH 7.0, 37C
0.0049
myricetin
-
pH 7.0 and 37C
0.0034
Naringenin
-
substrate vanillin, pH 7.0, 37C
0.0088
Naringenin
-
substrate phenanthridine, pH 7.0, 37C
0.00933
Naringenin
-
pH 7.0 and 37C
0.0251
Naringenin
-
substrate xanthine, pH 7.0, 37C
0.0004
quercetin
-
substrate vanillin, pH 7.0, 37C
0.0007
quercetin
-
substrate phenanthridine, pH 7.0, 37C; substrate xanthine, pH 7.0, 37C
0.00437
quercetin
-
pH 7.0 and 37C
0.0000029
raloxifene
-
-
12
raloxifene
-
in 25 mM phosphate buffer at 37C, pH not specified in the publication
0.0271
rutin
-
pH 7.0 and 37C
0.03802
silibinin
-
pH 7.0 and 37C
0.0041
taxifolin
-
substrate vanillin, pH 7.0, 37C
0.0147
taxifolin
-
substrate phenanthridine, pH 7.0, 37C
0.0285
taxifolin
-
substrate xanthine, pH 7.0, 37C
0.0389
vitexin
-
pH 7.0 and 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.0028
-
female liver
0.006
-
chromate reductase activity
0.0078
-
sulfoxide reductase activity
0.0094
-
-
0.0234
-
male liver
0.026
-
female liver treated with testosterone propionate; male liver treated with testosterone propionate
0.063
-
N1-methylnicotinamide oxidation with ferricyanide as electron acceptor
0.087
-
pH 7.8
0.11
-
-
0.12
-
ferricyanide reduction
0.137
-
-
0.155
-
tropylium ion oxidation with ferricyanide as electron acceptor
0.3
-
pH 7.6, specific activity increase with substrate concentration reaching a maximum around 0.035 mM benzaldehyde and decreases above
0.355
-
female enzyme untreated
0.553
-
female enzyme after treatment with testosterone propionate
0.562
-
recombinant enzyme
0.596
-
male enzyme
0.613
-
pH 7.8
0.75
-
-
0.8
-
2,6-dichlorophenol-indophenol under aerobic conditions as electron acceptor
0.8153
-
cytosolic enzyme
1.08
-
liver homogenate
1.8
-
1667fold purified enzyme
1.84
-
-
21.4
-
pH 7.0, 30C
188.3
-
231fold purified enzyme
additional information
P80456
in the case of all organic solvents, the activity of aldehyde oxidase decreases when the concentration of the solvent increases in the reaction medium
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6
-
substrate glyoxal
6
-
maximum aldehyde oxidase-catalyzed nitric oxide generation occurs at pH 6.0
7.4
-
assay at
7.4
-
assay at
7.5
-
-
7.7 - 9.7
-
-
7.8
-
assay at
7.8
-
-
7.8
-
assay at
8
S5FPI8
enzyme activity tested with three different buffers at pH 8, but no significant difference observed
9
P80456
phosphate buffer with no added organic solvent shows the highest aldehyde oxidase activity
9.5
-
-
10.5
-
-
additional information
P80456
the manner of the enzyme activity in the hydro-organic mixtures at different pHs is almost similar to that observed in the phosphate buffer system
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3 - 10
-
approx. 40% of maximal activity at pH 10
3 - 10
-
approx. 10% of maximal activity at pH 3.0, approx. 20% of maximal activity at pH 10
4 - 8
S5FPI8
-
4.1 - 10
-
approx. 5% of maximal activity at pH 4.0, approx. 10% of maximal activity at pH 10
5 - 8
-
maximum aldehyde oxidase-catalyzed nitric oxide generation occurs at pH 6.0. When the pH is decreased to 5.0 or increased above 8.0, a decrease in the rate of nitric oxide generation is observed
6 - 10
-
more than 90% activity
6 - 9
P80456
aldehyde oxidase active in either the organic solvent-free buffer or all nine water-miscible organic solvents, lowest enzyme activity is obtained at pH 6.0 with acetonitrile
7 - 8
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
-
benzaldehyde assay at
25
-
benzaldehyde assay at
30
-
assay at
30
-
assay at
37
-
N-[(2-dimethylamino)ethyl] acridine-4-carboxamide assay
40 - 45
-
-
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25 - 55
P80456
in the organic solvent-free buffer, a significant increase in the aldehyde oxidase activity observable as temperature raises up to 45C while enzyme activity decreases almost to zero when the reaction temperature is elevated to 55C
25 - 70
-
approx. 50% of maximal activity at 25C and 70C, respectively
25 - 70
-
approx. 50% of maximal activity at 25C and 65C, respectively
25 - 70
-
approx. 50% of maximal activity at 50C, approx. 20% of maximal activity at 70C
additional information
P80456
reduction in the enzyme activity at all temperatures in the presence of all organic solvents, except methanol and acetonitrile, enzyme activity in the presence of these two solvents increases with a rise in temperature up to 45C followed by a marked reduction at 55C, behavior of aldehyde oxidase is similar to that in the organic solvent free buffer solution
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
preadipocytes have a very low expression of AOX1, in 2 days differentiated cells AOX1 is induced and is not further upregulated in 3, 7 and 9 days differentiated cells. AOX1 mRNA is nearly four-fold higher in 2 days differentiated cells when compared to preadipocytes and doubles from days 2 to 6
Manually annotated by BRENDA team
-
isoform AOX1 mRNA is higher in visceral compared to subcutaneous adipose tissue, Aox1 protein is detected in both fat depots
Manually annotated by BRENDA team
Q06278
significantly expressed
Manually annotated by BRENDA team
S5FPI8
male and female
Manually annotated by BRENDA team
Q4VGM3
specific expression of enzyme in chemosensory organs, with strongest expression in antennae
Manually annotated by BRENDA team
A8TUB4, A8TUC0
AOX1 appears to be strongly expressed in antennae of both sexes, whereby intensities are clearly higher in male than in female antennae. It is predominantly expressed at the end of the pupal stage and during adult life. AOX1 is restricted to the sensilla side of the antennal branch
Manually annotated by BRENDA team
A8TUB4, A8TUC0
AOX2 appears to be strongly expressed in antennae of both sexes, whereby intensities are clearly higher in male than in female antennae. It is predominantly expressed at the end of the pupal stage and during adult life. AOX2 is restricted to the sensilla side of the antennal branch. AOX2 is associated in males with the bombykalsensitive sensilla
Manually annotated by BRENDA team
Bombyx mori Nistari
-
AOX1 appears to be strongly expressed in antennae of both sexes, whereby intensities are clearly higher in male than in female antennae. It is predominantly expressed at the end of the pupal stage and during adult life. AOX1 is restricted to the sensilla side of the antennal branch, AOX2 appears to be strongly expressed in antennae of both sexes, whereby intensities are clearly higher in male than in female antennae. It is predominantly expressed at the end of the pupal stage and during adult life. AOX2 is restricted to the sensilla side of the antennal branch. AOX2 is associated in males with the bombykalsensitive sensilla
-
Manually annotated by BRENDA team
-
trace amount
Manually annotated by BRENDA team
Q06278
weak expression
Manually annotated by BRENDA team
O54754, Q5SGK3, Q6V956, Q8VJ15
AOH2 and AOH3 mRNAs are expressed in the brain at much lower levels than AOX1 and AOH1
Manually annotated by BRENDA team
O54754, Q5SGK3, Q6V956, Q8VJ15
-
Manually annotated by BRENDA team
-
presence of the AOX1 transcript in the glial cell population of the spinal cord
Manually annotated by BRENDA team
O54754, Q5SGK3, Q6V956, Q8VJ15
richest source of AOH2 mRNA in the adult mouse is the inner ear
Manually annotated by BRENDA team
Mus musculus CD-1, Mus musculus DBA/2
-
-
-
Manually annotated by BRENDA team
O54754, Q5SGK3, Q6V956, Q8VJ15
AOH2 is also present
Manually annotated by BRENDA team
O54754, Q5SGK3, Q6V956, Q8VJ15
AOX1 mRNA is particularly abundant in the epithelial layer
Manually annotated by BRENDA team
O54754, Q5SGK3, Q6V956, Q8VJ15
AOH2
Manually annotated by BRENDA team
O54754, Q5SGK3, Q6V956, Q8VJ15
AOH2 and AOH3
Manually annotated by BRENDA team
Q2QB49, Q2QB50
-
Manually annotated by BRENDA team
Q06278
weak expression
Manually annotated by BRENDA team
-
hepatoma cell line, induction of enzyme isoform AOX1 by 2,3,7,8-tetrachlorodibenzo-p-dioxin
Manually annotated by BRENDA team
-
primary hepatocyte
Manually annotated by BRENDA team
Q06278
ABCA1 and AOX1 are coexpressed
Manually annotated by BRENDA team
-
highest activity
Manually annotated by BRENDA team
Q2QB49, Q2QB50
high level of enzyme mRNA
Manually annotated by BRENDA team
Q06278
significantly expressed
Manually annotated by BRENDA team
-
enzyme activity in wild-type, no activity in Moco sulfurase mutant flacca
Manually annotated by BRENDA team
Q06278
ABCA1 and AOX1 are coexpressed
Manually annotated by BRENDA team
-
high expression
Manually annotated by BRENDA team
-
male liver has about 7fold higher content of enzyme than that in female, gender specific regulation in the hepatic tissue
Manually annotated by BRENDA team
-
partial purification
Manually annotated by BRENDA team
-
partially purified
Manually annotated by BRENDA team
-
partially purified
Manually annotated by BRENDA team
-
enzyme isoform AOX1 and ite homologue AOH1 are induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin
Manually annotated by BRENDA team
-
induction of enzyme by high-fat diet, but not by free fatty acids or leptin
Manually annotated by BRENDA team
-
partial purification of enzyme
Manually annotated by BRENDA team
O54754, Q5SGK3, Q6V956, Q8VJ15
AOH2 is also present. Aldehyde oxidase activity shows higher levels in male than female adult mice
Manually annotated by BRENDA team
-
AOX1 is expressed at high levels
Manually annotated by BRENDA team
-
distribution of the activity is uneven, being seen mainly in the pericentral rather than the periportal area
Manually annotated by BRENDA team
-
high amounts
Manually annotated by BRENDA team
Q2QB49
only trace amounts of AOX1 activity
Manually annotated by BRENDA team
-
postnatal day 1, 7 and 14 rats show little or no liver aldehyde oxidase activity. Activity is markedly increased in liver cytosol from rats after postnatal day 14 and is then maintained up to 6 weeks
Manually annotated by BRENDA team
Q06278
strong AOX1 expression in normal liver, and in cirrhosis. Hepatocellular carcinomas show either a complete loss or reduced expression of AOX1
Manually annotated by BRENDA team
O54754, Q5SGK3, Q6V956, Q8VJ15
the AOH1 transcript is already detectable in newborn mice
Manually annotated by BRENDA team
O54754, Q5SGK3, Q6V956, Q8VJ15
the AOX1 transcript takes time to appear and is measurable only in the fully developed animal
Manually annotated by BRENDA team
Mus musculus CD-1, Rattus norvegicus SpragueDawley, Mus musculus DBA/2
-
-
-
Manually annotated by BRENDA team
-
high expression
Manually annotated by BRENDA team
Q06278
weak expression
Manually annotated by BRENDA team
-
AOX1 is expressed at high levels
Manually annotated by BRENDA team
Q2QB47, Q2QB48
only tissue examined with singificant levels of transcript
Manually annotated by BRENDA team
O54754, Q5SGK3, Q6V956, Q8VJ15
AOH2 and AOH3
Manually annotated by BRENDA team
-
selectively expressed in Bowman's glands
Manually annotated by BRENDA team
Q4VGM3
specific expression of enzyme in chemosensory organs, with strongest expression in antennae
Manually annotated by BRENDA team
Q06278
significantly expressed
Manually annotated by BRENDA team
O54754, Q5SGK3, Q6V956, Q8VJ15
AOH2
Manually annotated by BRENDA team
A8TUB4, A8TUC0
AOX1 slightly expressed
Manually annotated by BRENDA team
Bombyx mori Nistari
-
AOX1 slightly expressed
-
Manually annotated by BRENDA team
Q06278
in kidney proximal tubular epithelial cells, ABCA1 and AOX1 are coexpressed
Manually annotated by BRENDA team
-
enzyme activity in wild-type, no activity in Moco sulfurase mutant flacca
Manually annotated by BRENDA team
-
enzymic activity in mature nodules, preferential expression of enzyme in meristematic and invasion zones. Strong expression of isoform I, low expression of isoforms II and III
Manually annotated by BRENDA team
-
enzymic activity in young nodules, preferential expression of enzyme in in lateral meristemaitc zone. Strong expression of isoforms I and II, low expression of isoform III, preferentially expressed in lateral meristematic zone
Manually annotated by BRENDA team
-
preferetially expressed in invasion zone
Manually annotated by BRENDA team
-
high and specific expression in developing seed
Manually annotated by BRENDA team
-
in mature dry seeds, aldehyde oxidase activity and expression of is restricted to the embryonic tissues
Manually annotated by BRENDA team
-
during the early stages of seed development, the aldehyde oxidase activity in seeds is almost exclusively restricted to the seed coat
Manually annotated by BRENDA team
O54754, Q5SGK3, Q6V956, Q8VJ15
AOH2
Manually annotated by BRENDA team
Mus musculus CD-1, Mus musculus DBA/2
-
-
-
Manually annotated by BRENDA team
Q06278
weak expression
Manually annotated by BRENDA team
-
AOX1 is expressed at high levels
Manually annotated by BRENDA team
Q06278
weak expression
Manually annotated by BRENDA team
Q2QB47, Q2QB48
high levels of transcript
Manually annotated by BRENDA team
O54754, Q5SGK3, Q6V956, Q8VJ15
-
Manually annotated by BRENDA team
Q06278
significantly expressed
Manually annotated by BRENDA team
O54754, Q5SGK3, Q6V956, Q8VJ15
very large amounts of AOH2 are predicted to be present during the early stages of development and specifically in the zygote
Manually annotated by BRENDA team
-
high amounts
Manually annotated by BRENDA team
additional information
-
no activity in liver
Manually annotated by BRENDA team
additional information
-
detectable amounts of enzyme also in eye, kidney, thymus, testis, duodenum, heart and esophagus
Manually annotated by BRENDA team
additional information
Q2QB49, Q2QB50
no enzymic activity in liver
Manually annotated by BRENDA team
additional information
-
no enzymic activity in liver
Manually annotated by BRENDA team
additional information
O54754, Q5SGK3, Q6V956, Q8VJ15
AOH2 is abundant in the Harderian gland. AOH3 is restricted to the Bowman's gland
Manually annotated by BRENDA team
additional information
-
completely devoid of liver aldehyde oxidase activity
Manually annotated by BRENDA team
additional information
Q06278
in adrenocortical cells, ABCA1 and AOX1 are coexpressed. Absent from skeletal muscle, small intestine, MCF-7, PC3, CaCo-2, and HT-29 cells
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
95000
-
PAGE
699045
132000
-
gel filtration
644705
140000
-
gel filtration
656311
140000
S5FPI8
SDS-PAGE, AtraAOX2 monomer still shows strong activity
726341
141800
A8TUB4, A8TUC0
sequence analysis
692468
142000
-
gel filtration
656311
143300
A8TUB4, A8TUC0
sequence analysis
692468
148000
-
gel filtration
656311
150000
Q2QB49, Q2QB50
gel filtration
674600
150000
-
gel filtration
677264
150000
-
native-PAGE/Western blot analysis. Slc:Wistar, Crl:SD, F344/DuCrlCrlj, and Slc:SD strains
695270
150000
-
SDS-PAGE, native and recombinant protein, degradation products of 130000, 80000, 70000, and 55000 Da in case of purified recombinant mAOX3
724827
160000
-
native PAGE
654321
200000
-
gel filtration
390375
200000
-
gel filtration
675237
222000
-
gel filtration
390387
250000
-
gel filtration
691162
260000
-
disc gel electrophoresis
390412
265000
-
non-denaturating PAGE
390379
270000 - 280000
-
sedimentation equilibrium
390413
270000
-
disc gel electrophoresis
390412
270000
-
gel filtration
390423
280000
-
gel filtration
390410
280000
-
sedimentation equilibrium
390417
280000
S5FPI8
Western blot analysis
726341
288000
-
gel filtration
390376
290000
-
gel filtration
690612
300000
-
PAGE
390370
300000
-
gel filtration
390381
300000
-
gel filtration
390382
300000
-
PAGE
390390
300000
-
gel filtration
390396
300000
-
gel filtration
390422
300000
-
Western blot analysis
691162
300000
-
native-PAGE/Western blot analysis. WKAH/Hkm, WKY/Izm, LEW/CrlCrlj, Crlj:WI, Jcl:Wistar, and Wistar-Imamichi strains
695270
300000
-
gel filtration, native and recombinant mAOX3
724827
302000
Q9NCL9
native PAGE
348684
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 150000, SDS-PAGE
?
-
x * 150000, immunoblotting
?
-
x * 83000 + x * 38000 + x * 18000, SDS-PAGE
?
-
x * 140000, isoform I, x * 145000, isoform II, SDS-PAGE
?
-
x * 140000, isoform II, x * 145000, isoform III, SDS-PAGE. Isoform I shows faster mobility in SDS-PAGE
?
-
x * 130000, SDS-PAGE, x * 150000, Coomassie brilliant blue-staining
?
-
x * 150000, SDS-PAGE, recombinant protein
?
Pseudomonas stutzeri 12695
-
x * 83000 + x * 38000 + x * 18000, SDS-PAGE
-
dimer
-
2 * 135000, SDS-PAGE
dimer
-
2 * 100000, SDS-PAGE
dimer
-
2 * 160000, SDS-PAGE
dimer
-
alpha2, 2 * 100000, SDS-PAGE
dimer
-
alpha2, 2 * 144800, SDS-PAGE
dimer
-
alpha2, 2 * 150000, SDS-PAGE
dimer
-
alpha2, 2 * 150000, SDS-PAGE
dimer
-
alpha2, 2 * 150000, SDS-PAGE
dimer
-
alpha2, 2 * 138000, SDS-PAGE
dimer
-
alpha2, 2 * 148000, SDS-PAGE
dimer
-
alpha2, 2 * 140000, SDS-PAGE
dimer
-
2 * 145000, SDS-PAGE
dimer
-
1 * 160000 + 1 * 145000, isoenzyme AO3, SDS-PAGE, 2 * 145000, isoenzyme AO4, SDS-PAGE
dimer
Q9NCL9
2 * 150000, deduced from nucleotide sequence
dimer
Desulfovibrio aminophilus DSM12254
-
2 * 100000, SDS-PAGE
-
heterotrimer
-
1 * 79000, 1 * 39000, 1 * 23000, SDS-PAGE
heterotrimer
Streptomyces rimosus ATCC10970
-
1 * 79000, 1 * 39000, 1 * 23000, SDS-PAGE
-
homodimer
S5FPI8
2 * 140000, SDS-PAGE
homodimer
-
native-PAGE/Western blot analysis. WKAH/Hkm, WKY/Izm, LEW/CrlCrlj, Crlj:WI, Jcl:Wistar, and Wistar-Imamichi strains
homodimer
-
2 * 150000, gel filtration
homodimer
G3X982
each monomer comprises 1335 residues and can be divided into three major domains, a small 20000 Da N-terminal domain that harbors the two FeS clusters and connects to the 40000 Da FAD binding domain and finally the C-terminal 90000 Da domain containing the Moco binding site, with the molybdenum catalytic center
monomer
-
1 * 150000, SDS-PAGE
monomer
Q2QB49, Q2QB50
1 * 150000, SDS-PAGE and calculated
monomer
-
native-PAGE/Western blot analysis. Slc:Wistar, Crl:SD, F344/DuCrlCrlj, and Slc:SD strains
tetramer
-
4 * 27000, SDS-PAGE
tetramer
-
4 * 27000, SDS-PAGE
-
trimer
-
x-ray crystallography
trimer
-
1 * 85000 + 1 * 39000 + 1 * 19000, SDS-PAGE
trimer
-
1 * 86000 + 1 * 37000 + 1 * 23000, SDS-PAGE
trimer
-
1 * 88000 + 1 * 38000 + 1 * 18000, SDS-PAGE
trimer
-
1 * 88000 + 1 * 39000 + 1 * 18000, SDS-PAGE
trimer
Pseudomonas sp. KY 469, Pseudomonas sp. KY 4690
-
1 * 88000 + 1 * 39000 + 1 * 18000, SDS-PAGE
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 0.1 M sodium acetate buffer pH 4.6 and 6% (w/v) PEG 4000, at 20C
-
2.8 A resolution, 98% complete
-
to 2.9 A resolution, used for elucidation of putative reaction mechanism of aldehyde oxidases
G3X982
vast majority of the crystallization trials performed using the recombinant protein, usable data set of crystals of native mAOX3 with a resolution of 2.9 A
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3 - 5
-
no loss of activity after 15 min at 55C
656311
3
-
complete loss of activity after 15 min at 55C
656311
4
-
complete loss of activity after 15 min at 55C
656311
4.9 - 9.5
-
-
390375
5 - 9
-
no loss of activity after 15 min at 55C
656311
6 - 9
-
stable within this range
677264
7 - 8.5
-
30C, retains more than 80% of initial activity for at least 2 h
699045
7 - 9
-
-
390385
7
-
no loss of activity after 30 min at 70C
644705
10
-
approx. 40% loss of activity after 15 min at 55C
656311
11
-
complete loss of activity after 15 min at 55C
656311
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
20 - 60
S5FPI8
relatively stable from 20 to 60C but activity drops dramatically at 70C, AtraAOX2 preheated at 60C, shows biphasic inactivation, with a rapid decrease from 0 to 10 min, and monotonous decrease thereafter, with an estimated half-life of 33 min
726341
30 - 55
-
enzyme retains at least 80% of initial activity for 30 min
699045
35
P80456
the activity remains almost unchanged after 24 h in the absence of organic solvents at 35C, enzyme is relatively stable in the presence of methanol, 2-propanol and acetonitrile
723978
55
P80456
at 55C, the enzyme undergoes a conformational change producing an inactive form, conversion is reversible
723978
60 - 70
-
relatively stable
390381
60
-
4% loss of activity after 30 min
654321
60
-
approx. 10% loss of activity after 15 min at pH 7.0
656311
70
-
no loss of activity after 30 min at pH 7.0
644705
70
-
8% loss of activity after 30 min
654321
70
-
approx. 50% loss of activity after 15 min at pH 7.0
656311
70
-
less than 10% loss of activity after 15 min at pH 7.0
656311
70
-
stable below for at least 30 min
677264
80
-
5% loss of activity after 30 min at pH 7.0
644705
80
-
50% loss of activity after 30 min
654321
80
-
approx. 85% loss of activity after 15 min at pH 7.0
656311
80
-
approx. 15% loss of activity after 15 min at pH 7.0
656311
80
-
approx. 40% loss of activity after 15 min at pH 7.0
656311
100
-
after 5 min complete activity loss
390381
additional information
P80456
incubation of aldehyde oxidase in all water-miscible organic solvents results in a marked loss of the catalytic activity, residual activity of aldehyde oxidase in the presence of dioxane, ethanol, N,N-dimethylformamide, and pyridine is 16%, 10%, 12%, and 17% after 24 h at 35C
723978
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme inactivated by KCN treatment
-
immobilization on n-octylamine Sepharose 4B and gelatin cross-linked with glutaraldehyde, low operational and storage stability, low product yield
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Methanol
-
27% activity with 2% v/v methanol
N,N-dimethylformamide
P80456
concentration of half inactivation = 13.3%
Pyridine
P80456
concentration of half inactivation = 0.5%
tetrahydrofuran
P80456
concentration of half inactivation = 0.5%
Methanol
P80456
concentration of half inactivation = 0.5%
additional information
P80456
in the case of all organic solvents, the activity of aldehyde oxidase decreased when the concentration of the solvent increased in the reaction medium
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, 25 mM Tris-HCl buffer, pH 8.2, 19 days, 100% activity retained
-
ammonium ions, DTT and FAD protect against inactivation during storage
-
-20C, 1 month, 100% activity retained
-
30C, pH 7.8, phosphate or diphosphate buffer, 4 days, 50% activity
-
30C, pH 9.0, diphosphate or borate buffer, 13 days, 50% activity
-
30C, pH 9.0, Tris-HCl buffer, 17 days, 50% activity
-
4C, pH 7.8, phosphate or diphosphate buffer, 34 days, 50% activity
-
4C, pH 9.0, borate buffer, 108 days, 50% activity
-
4C, pH 9.0, diphosphate buffer, 87 days, 50% activity
-
4C, pH 9.0, Tris-HCl buffer, 245 days, 50% activity
-
storage leeds to polymerization, prevention is achieved with thiol reagents like 5 mM cysteine
-
-70C, stable for 6 months
-
storage leeds to polymerization, prevention is achieved with thiol reagents like 5 mM cysteine
-
-30C, 20 mM Tris-HCl buffer, pH 8.0
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by Ni-NTA column chromatography
S5FPI8
Ni Sepharose 6 Fast Flow column chromatography, HiTrap QHP column chromatography, and HisTrap FF column chromatography
-
ammonium sulfate precipitation
-
partial purification
-
partially purified
-
-
Q2QB49, Q2QB50
231fold purified at a yield of about 1% by heat treatment, ammonium sulfate fractionation, column chromatographies, and hydroxyapatite
-
Ni affinity column chromatography
-
55C, ammonium sulfate, Mono Q
-
; 55C, ammonium sulfate, Benzmidine-Sepharose, Mono Q
-
mAOX3 purified from CD1 mouse liver as well as from a heterologous expression system from Escherichia coli, recombinant mAOX3 expressed as an N-terminal fusion protein with a His6 tag
G3X982
recombinant mAOX3 purified using sequential Ni-NTA chromatography and size exclusion chromatography, a chemical sulfuration step performed to further increase the activity of the enzyme 1.4fold and after coexpression with mMCSF and chemical sulfuration, 30% of recombinant mAOX3 exists in the catalytically active form, native mAOX3 purified by ammonium sulfate precipitation with 50% saturation, benzamidine Sepharose chromatography and a linear NaCl gradient on a 5/5 FPLC Mono Q column
-
to homogeneity, chromatography techniques
-
Ni affinity column chromatography
-
partially purified by heat treatment and ammonium sulfate precipitation
P80456
to homogeneity, chromatography techniques
-
heat treatment, Butyl-toyopearl, Resource Q
-
1667fold by gel filtration with a yield of 12%, to more than 98% purity
-
partial purification by heat treatment and ammonium sulfate precipitation
-
simultaneous purification with xanthine oxidase
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression of recombinant AtraAOX2 with His6-tag at the C-terminal end in a baculovirus expression system
S5FPI8
expression in Pichia pastoris of the AOalpha gene
-
expressed in Escherichia coli BL21(DE3) cells
-
;
A8TUB4, A8TUC0
cloning of cDNA
Q9NCL9
expression in Escherichia coli
-
expressed in Escherichia coli M15 cells
-
into the pQE30Xa vector and expressed in Escherichia coli M15 (pREP4) cells
-
AOX1 expressed in Escherichia coli
O54754, Q5SGK3, Q6V956, Q8VJ15
cDNA of mAOX3 cloned from mouse CD1 liver into pMMA1 plasmid and expression as an N-terminal fusion protein with a His6 tag, coexpression with mMCSF, heterologous expression in Escherichia coli
-
cloning of cDNA
-
expression in Escherichia coli
-
transfection into HC11 mouse mammary epithelial cell line
-
expressed in Escherichia coli M15 cells
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
isoform AO1 transcripts are at their highest level from 3 to 8 days after flowering
B0LAZ9, B0LB00, B0LB01
the level of isoform AO2 expression is high at the early stages of embryo development (from 3 to 8 days after flowering)
B0LAZ9, B0LB00, B0LB01
the transcript level of isoform AO3 reaches a maximum between 7 and 10 days after flowering
B0LAZ9, B0LB00, B0LB01
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
A1023Y
-
the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme
A1083T
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the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme
A1083T/V1085A
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the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme
I1032V
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the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme
K1004Q
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the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme
K1004Q/K1005R
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the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme
K1004Q/K1005R/M1009I/V1010I
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the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme
K1004Q/K1005R/M1009I/V1010I/R1021V/A1023Y
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the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme