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Information on EC 1.2.1.12 - glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) and Organism(s) Plasmodium falciparum

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IUBMB Comments
Also acts very slowly on D-glyceraldehyde and some other aldehydes; thiols can replace phosphate.
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Plasmodium falciparum
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The taxonomic range for the selected organisms is: Plasmodium falciparum
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
gapdhs, d-glyceraldehyde-3-phosphate dehydrogenase, gapds, gadph, glyceraldehyde-3-phosphate dehydrogenases, plasmin receptor, gapc1, plasminogen-binding protein, gapcp, glyceraldehyde-3 phosphate dehydrogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3-phosphoglyceraldehyde dehydrogenase
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-
-
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BARS-38
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-
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CP 17/CP 18
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-
-
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D-glyceraldehyde-3-phosphate dehydrogenase
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dehydrogenase, glyceraldehyde phosphate
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-
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dihydrogenase, glyceraldehyde phosphate
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-
-
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G3PD
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-
-
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GAPDH
GAPDH1
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-
-
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GAPDH2
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-
-
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glyceraldehyde phosphate dehydrogenase (NAD)
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-
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glyceraldehyde-3-P-dehydrogenase
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-
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glyceraldehyde-3-phosphate dehydrogenase
glyceraldehyde-3-phosphate dehydrogenase (NAD)
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-
-
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GPD
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-
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Gra3PDH
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-
-
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GraP-DH
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-
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Larval antigen OVB95
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-
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Major larval surface antigen
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-
-
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NAD+-G-3-P dehydrogenase
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-
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NAD-dependent glyceraldehyde phosphate dehydrogenase
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-
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NAD-dependent glyceraldehyde-3-phosphate dehydrogenase
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NAD-G3PDH
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-
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NADH-glyceraldehyde phosphate dehydrogenase
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-
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P-37
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PfGAPDH
phosphoglyceraldehyde dehydrogenase
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-
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Plasmin receptor
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-
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Plasminogen-binding protein
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-
-
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TLAb
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-
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triose phosphate dehydrogenase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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Phosphorylation
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redox reaction
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-
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reduction
SYSTEMATIC NAME
IUBMB Comments
D-glyceraldehyde-3-phosphate:NAD+ oxidoreductase (phosphorylating)
Also acts very slowly on D-glyceraldehyde and some other aldehydes; thiols can replace phosphate.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-50-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ferriprotoporphorin IX
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ferriprotoporphyrin IX
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.25
D-glyceraldehyde 3-phosphate
recombinant enzyme, pH and temperature not specified in the publication
0.28
NAD+
recombinant enzyme, pH and temperature not specified in the publication
additional information
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
12.6
purified recombinant enzyme, pH and temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
recombinant enzyme
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.77
recombinant wild-type enzyme, sequence calculation and isoelectric focusing
8.33
recombinant N-terminally prolongated enzyme, sequence calculation and isoelectric focusing
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
enzyme Gapdh is likely to have multiple nonglycolytic functions in the parasite in additon to its function in glycolysis. During intra-erythrocytic phage of its life cycle in humans, the parasite solely relies on glycolysis for its energy needs
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
O96369_PLAFA
337
0
36637
TrEMBL
Mitochondrion (Reliability: 2)
Q8T6B1_PLAFA
337
0
36635
TrEMBL
Mitochondrion (Reliability: 2)
B0F3R3_PLAFA
320
0
34843
TrEMBL
Mitochondrion (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
130000
-
tetramer, estimated by gel filtration
150000
recombinant N-terminally prolongated enzyme, gel filtration
161984
recombinant N-terminally prolongated enzyme, mass spectrometry
36000
-
monomer
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystals of tag-free protein diffracts X-rays to 2.6 A resolution, of the His-tagged protein to 4.0 A
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the crystal structure is solved at 2.25 A resolution
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, HEPES buffer, pH 7.25, 500 mM NaCl, 5% glycerol, 2 mM DTT, 0.025% sodium azide
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified on Superflow NTA nickel resin and gel chromatography, Superdex 75 26/60
recombinant His6-tagged enzyme 2.8fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
the filtered supernatant of the centrifuged cell lysate is applied onto 3 ml Talon resin, the His-tag is removed, further purification by gel filtration
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Gapdh gene is located on chromosome 14, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), addition of 36 amino acid residues (MGS SHHHHHHSSGLVPRGSHMASMTGGNNMGRGSGF-) at the N-terminus of rPfGapdh
into the pET14b vector for expression in Escherichia coli BL21 Star cells
into the pET15b vector for expression in Escherichia coli BL21DE3 cells
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
Gapdh can be an effective target for anti-malarial chemotherapeutics. Enzyme PfGapdh shows an extra ligand binding capacity in the vicinity of the NAD+ binding region of the active site that may offer an opportunity to design and develop novel antimalarials
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Satchell, J.F.; Malby, R.L.; Luo, C.S.; Adisa, A.; Alpyurek, A.E.; Klonis, N.; Smith, B.J.; Tilley, L.; Colman, P.M.
Structure of glyceraldehyde-3-phosphate dehydrogenase from Plasmodium falciparum
Acta Crystallogr. Sect. D
D61
1213-1221
2005
Plasmodium falciparum
Manually annotated by BRENDA team
Robien, M.A.; Bosch, J.; Buckner, F.S.; Van Voorhis, W.C.; Worthey, E.A.; Myler, P.; Mehlin, C.; Boni, E.E.; Kalyuzhniy, O.; Anderson, L.; Lauricella, A.; Gulde, S.; Luft, J.R.; DeTitta, G.; Caruthers, J.M.; Hodgson, K.O.; Soltis, M.; Zucker, F.; Verlinde, C.L.; Merritt, E.A.; Schoenfeld, L.W.; Hol, W.G.
Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Plasmodium falciparum at 2.25 A resolution reveals intriguing extra electron density in the active site
Proteins
62
570-577
2006
Plasmodium falciparum (Q8IKK7), Plasmodium falciparum
Manually annotated by BRENDA team
Akinyi, S.; Gaona, J.; Meyer, E.V.; Barnwell, J.W.; Galinski, M.R.; Corredor, V.
Phylogenetic and structural information on glyceraldehyde-3-phosphate dehydrogenase (G3PDH) in Plasmodium provides functional insights
Infect. Genet. Evol.
8
205-212
2008
Plasmodium brasilianum (B0F3R1), Plasmodium coatneyi (B0F3R2), Plasmodium falciparum (B0F3R3), Plasmodium fragile (B0F3R4), Plasmodium knowlesi strain H (B0F3R5), Plasmodium malariae (B0F3R6), Plasmodium reichenowi (B0F3R7), Plasmodium cynomolgi (B0F3R8), Plasmodium vivax (B0F3R9), Plasmodium fragile Nilgiri (B0F3R4), Plasmodium vivax Salvador I (B0F3R9), Plasmodium reichenowi CDC1 (B0F3R7), Plasmodium brasilianum Peru II (B0F3R1), Plasmodium malariae Uganda I (B0F3R6), Plasmodium cynomolgi Berok (B0F3R8), Plasmodium falciparum FVO (B0F3R3)
Manually annotated by BRENDA team
Sangolgi, P.B.; Balaji, C.; Dutta, S.; Jindal, N.; Jarori, G.K.
Cloning, expression, purification and characterization of Plasmodium spp. glyceraldehyde-3-phosphate dehydrogenase
Protein Expr. Purif.
117
17-25
2016
Plasmodium falciparum (Q8T6B1), Plasmodium falciparum, Plasmodium yoelii (V7PVE5), Plasmodium yoelii, Plasmodium yoelii 17XL (V7PVE5)
Manually annotated by BRENDA team