Information on EC 1.14.99.10 - steroid 21-monooxygenase

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The expected taxonomic range for this enzyme is: Euteleostomi

EC NUMBER
COMMENTARY
1.14.99.10
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RECOMMENDED NAME
GeneOntology No.
steroid 21-monooxygenase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
a C21 steroid + [reduced NADPH-hemoprotein reductase] + O2 = a 21-hydroxy-C21-steroid + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydroxylation
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oxidation
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redox reaction
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reduction
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PATHWAY
KEGG Link
MetaCyc Link
Metabolic pathways
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Steroid hormone biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
steroid,NADPH-hemoprotein reductase:oxygen oxidoreductase (21-hydroxylating)
Requires NADPH and EC 1.6.2.4, NADPH---hemoprotein reductase. A heme-thiolate protein (P-450) enzyme responsible for the conversion of progesterone and 17alpha-hydroxyprogesterone to their respective 21-hydroxylated derivatives, 11-deoxycorticosterone and 11-deoxycortisol. Involved in the biosynthesis of the hormones aldosterone and cortisol.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
17alpha-hydroxyprogesterone 21-hydrolase
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21-hydroxylase
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21-hydroxylase
P08686
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21-hydroxylase cytochrome P-450
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21OH
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21OH
P08686
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CYP21
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CYP21
P08686
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CYP21A2
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Cytochrome P-450 specific for steroid C-21 hydroxylation
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cytochrome P-450-linked mixed function oxidase system
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cytochrome P-450C-21
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cytochrome P450 steroid 21-hydroxylase
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EC 1.14.1.8
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formerly
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EC 1.99.1.11
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formerly
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P-450(C21)
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P450 oxidoreductase-21-hydroxylase
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P450-C21
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P450-C21B
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P450c21
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P450c21
P00191
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P450c21
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Steroid 21-hydroxylase
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Steroid 21-hydroxylase
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steroid 21-hydroxylase system
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steroid 21-hydroxylation system
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steroid cytochrome P450 21-hydroxylase
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steroid cytochrome P450 21-hydroxylase
P08686
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CAS REGISTRY NUMBER
COMMENTARY
9029-68-9
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
expressed in Escherichia coli
SwissProt
Manually annotated by BRENDA team
expression in human embryonic kidney 293 cells
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Manually annotated by BRENDA team
expression in COS-1 cells
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Manually annotated by BRENDA team
gene CYP21A2
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Manually annotated by BRENDA team
Italian patients with congenital adrenal hyperplasia
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Manually annotated by BRENDA team
Middle European patients with congenital adrenal hyperplasia
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Manually annotated by BRENDA team
rainbow trout
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Manually annotated by BRENDA team
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
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Bovine serum albumin
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Cymal 5
P00191
optimal concentration is 0.002%, decrease in activity above 0.05%
dithiothreitol
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Emulgen 911
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Emulgen 913
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maximum with 0.008% v/v
L-cysteine
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L-cystine
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lysophosphatidylcholine
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Sodium cholate
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Sodium deoxycholate
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Triton X-100
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TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.322
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17a-hydroxyprogesterone
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0.172
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progesterone
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PDB
SCOP
CATH
ORGANISM
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
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3.6% carbohydrate
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-70°C, 0.2 M potassium phospate, pH 7.4, 20% glycerol, 0.1 mM EDTA, 3 months without loss of activity
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0°C, 50 mM Tris, pH 7.2, 20% glycerol, 0.15% emulgen, 0.1 mM dithiothreitol, 0.1 mM EDTA, several weeks spectroscopically stable
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25°C, 50 mM Tris, pH 7.2, 20% glycerol, 0.15% emulgen, 0.1 mM dithiothreitol, 0.1 mM EDTA, several hours spectroscopically stable, without Emulgen 50% precipitation after 30 min
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