Information on EC 1.14.19.2 - acyl-[acyl-carrier-protein] desaturase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
1.14.19.2
-
RECOMMENDED NAME
GeneOntology No.
acyl-[acyl-carrier-protein] desaturase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
a stearoyl-[acyl-carrier protein] + reduced acceptor + O2 = an oleoyl-[acyl-carrier protein] + acceptor + 2 H2O
show the reaction diagram
specific for stearoyl-CoA
P22243
a stearoyl-[acyl-carrier protein] + reduced acceptor + O2 = an oleoyl-[acyl-carrier protein] + acceptor + 2 H2O
show the reaction diagram
enzyme acts on derivatives of a number of long-chain fatty acids, requires ferredoxin, formerly EC 1.14.99.6
-
a stearoyl-[acyl-carrier protein] + reduced acceptor + O2 = an oleoyl-[acyl-carrier protein] + acceptor + 2 H2O
show the reaction diagram
peroxodiferric intermediate is suggested
-
a stearoyl-[acyl-carrier protein] + reduced acceptor + O2 = an oleoyl-[acyl-carrier protein] + acceptor + 2 H2O
show the reaction diagram
EPR and ENDOR studies, both major conformers of the diferric cluster have a mu-oxo bridge, structural alterations upon binding of substrate
-
a stearoyl-[acyl-carrier protein] + reduced acceptor + O2 = an oleoyl-[acyl-carrier protein] + acceptor + 2 H2O
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
oxidation
A8D2K7
-
redox reaction
-
-
-
-
reduction
-
-
-
-
reduction
A8D2K7
-
PATHWAY
KEGG Link
MetaCyc Link
Biosynthesis of unsaturated fatty acids
-
Fatty acid biosynthesis
-
oleate biosynthesis I (plants)
-
SYSTEMATIC NAME
IUBMB Comments
acyl-[acyl-carrier protein],hydrogen-donor:oxygen oxidoreductase
The enzyme from safflower is specific for stearoyl-[acyl-carrier protein]; that from Euglena acts on derivatives of a number of long-chain fatty acids. Requires ferredoxin.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
acyl-ACP-desaturase
O53442, Q50824
-
acyl-ACP-desaturase
Mycobacterium tuberculosis H37Rv,variant DesA2
O53442
-
-
ahSAD3A
-
-
ahSAD3B
-
-
DesA2
Mycobacterium tuberculosis H37Rv,variant DesA2
O53442
-
-
desaturase, acyl-[acyl carrier protein]
-
-
-
-
EC 1.14.99.6
-
-
formerly
-
S-ACP-DES
O22832
-
S-ACP-DES1
Q9LF04
-
S-ACP-DES2
Q6NQK5
-
S-ACP-DES3
Q9LF05
-
S-ACP-DES4
Q9M880
-
S-ACP-DES5
Q8L468
-
S-ACP-DES6
Q84VY3
-
SSI2 desaturase
O22832
-
SSI2/FAB2
O22832
-
stearoyl-ACP desaturase
-
-
-
-
stearoyl-ACP desaturase
-
-
stearoyl-acyl carrier protein desaturase
O22832
-
stearoyl-acyl carrier protein desaturase
-
-
stearoyl-acyl carrier protein desaturase
A8D2K7
-
stearoyl-acyl carrier protein desaturase
-
-
stearoyl-[acyl carrier protein] desaturase
-
-
-
-
stearyl acyl carrier protein desaturase
-
-
-
-
stearyl-ACP desaturase
-
-
-
-
stearyl-acyl carrier protein desaturase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37256-86-3
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
-
Q6NQK5, Q8L468, Q9LF04, Q9LF05, Q9M880
SwissProt
Manually annotated by BRENDA team
seven isoforms
SwissProt
Manually annotated by BRENDA team
milkweed
-
-
Manually annotated by BRENDA team
Bignonia unguis-cati
cat's claw
SwissProt
Manually annotated by BRENDA team
safflower
SwissProt
Manually annotated by BRENDA team
coriander
-
-
Manually annotated by BRENDA team
oil palm, clone 1
-
-
Manually annotated by BRENDA team
oil palm, clone 2
SwissProt
Manually annotated by BRENDA team
soybean
-
-
Manually annotated by BRENDA team
mutant strain showing contents of up to 20% of unusual acyl chains, increase of enzyme activity in mutant
-
-
Manually annotated by BRENDA team
H37Rv, variants DesA1
SwissProt
Manually annotated by BRENDA team
H37Rv,variant DesA2
SwissProt
Manually annotated by BRENDA team
Mycobacterium tuberculosis H37Rv,variant DesA2
H37Rv,variant DesA2
SwissProt
Manually annotated by BRENDA team
avocado
-
-
Manually annotated by BRENDA team
castor bean
SwissProt
Manually annotated by BRENDA team
black-eyed Susan vine
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
Ophrys SAD coding sequences are heterologously expressed in Arabidopsis under the control of the Cauliflower mosaic virus 35S RNA promoter. None of the transgenic plant lines complement the dwarf phenotype of homozygous ssi2 mutants. The presence of the OsSAD2 transgene is significantly associated with changes in unsaturated C18 and C16 FA levels in Arabidopsis leaf lipids, suggesting that OsSAD2 has enzymatic activity in Arabidopsis; Ophrys SAD coding sequences are heterologously expressed in Arabidopsis under the control of the Cauliflower mosaic virus 35S RNA promoter. OeSAD2 does not complement the dwarf phenotype of homozygous ssi2 mutants; Ophrys SAD coding sequences are heterologously expressed in Arabidopsis under the control of the Cauliflower mosaic virus 35S RNA promoter. OsSAD1 does not complement the dwarf phenotype of homozygous ssi2 mutants
physiological function
-
SAD2 is a florally expressed barrier gene of large phenotypic effect and, possibly, a genic target of pollinator-mediated selection
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
10-(heptyloxy)-decanoyl-[acyl-carrier protein] + reduced acceptor + O2
9-hydroxynonanoyl-[acyl-carrier protein] + n-octanal + acceptor + H2O
show the reaction diagram
-
-
-
?
7-(decyloxy)-heptanoyl-[acyl-carrier protein] + reduced acceptor + O2
7-dec-1-enyloxyheptanoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
-
-
?
8-(nonyloxy)-octanoyl-[acyl-carrier protein] + reduced acceptor + O2
8-hydroxyoctanoyl-[acyl-carrier protein] + 1-nonanal + acceptor + H2O
show the reaction diagram
-
-
-
?
9-(octyloxy)-nonanoyl-[acyl-carrier protein] + reduced acceptor + O2
9-hydroxynonanoyl-[acyl-carrier protein] + 1-octanal + acceptor + H2O
show the reaction diagram
-
-
-
?
nonadecanoyl-[acyl-carrier protein] + reduced ferredoxin + O2
9-nonadecenoyl-[acyl-carrier protein] + oxidized ferredoxin + H2O
show the reaction diagram
-
low activity, vegetative ferredoxin from Anabaena
-
?
palmitoyl-[acyl-carrier protein] + AH2 + O2
palmitoleoyl-[acyl-carrier protein] + A + H2O
show the reaction diagram
O22832, Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
preferred substrate for S-ACP-DES3, S-ACP-DES3 preferentially desaturates palmitoyl-[acyl-carrier protein] substrate at C9 position
-
-
?
palmitoyl-[acyl-carrier protein] + AH2 + O2
palmitoleoyl-[acyl-carrier protein] + A + H2O
show the reaction diagram
O22832, Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
S-ACP-DES1 is poorly active on palmitoyl-[acyl-carrier protein], S-ACP-DES1 preferentially desaturates the substrate at C9 position
-
-
?
palmitoyl-[acyl-carrier protein] + AH2 + O2
palmitoleoyl-[acyl-carrier protein] + A + H2O
show the reaction diagram
O22832, Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
S-ACP-DES4 is poorly active on palmitoyl-[acyl-carrier protein], S-ACP-DES4 preferentially desaturates the substrate at C9 position
-
-
?
palmitoyl-[acyl-carrier protein] + AH2 + O2
palmitoleoyl-[acyl-carrier protein] + A + H2O
show the reaction diagram
O22832, Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
S-ACP-DES5 poorly active on palmitoyl-[acyl-carrier protein], S-ACP-DES5 preferentially desaturates palmitoyl-[acyl-carrier protein] substrate at C9 position
-
-
?
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
9-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
O24498
-
-
-
?
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
9-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
-
-
-
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
9-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
-
-
?
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
9-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
-
-
?
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
9-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
8% of activity with stearoyl-[acyl-carrier-protein], 7fold higher activity than the enzyme from castor
-
?
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
9-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
Bignonia unguis-cati
P92933
4fold lower activity with stearoyl-[acyl-carrier protein] and myristoyl-[acyl-carrier protein]
-
?
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
9-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
1% of activity with stearoyl-[acyl-carrier-protein]
-
?
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
9-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
stearoyl-substrate is about 100fold preferred over palmitoyl-substrate
-
-
?
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
6-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
-
-
?
pentadecanoyl-[acyl-carrier protein] + reduced ferredoxin + O2
9-pentadecenoyl-[acyl-carrier protein] + oxidized ferredoxin + H2O
show the reaction diagram
-
very low activity, vegetative ferredoxin from Anabaena
-
?
stearoyl-CoA + electron donor + O2
9-octadecenoyl-CoA + acceptor + H2O
show the reaction diagram
P22243
-
-
-
stearoyl-CoA + electron donor + O2
9-octadecenoyl-CoA + acceptor + H2O
show the reaction diagram
-
-
-
-
stearoyl-CoA + electron donor + O2
9-octadecenoyl-CoA + acceptor + H2O
show the reaction diagram
-
purified reconstituted enzyme shows same activity as with stearoyl-[acyl-carrier-protein], no activity in crude extracts
-
?
stearoyl-CoA + electron donor + O2
9-octadecenoyl-CoA + acceptor + H2O
show the reaction diagram
-
5% of activity with stearoyl-[acyl-carrier-protein]
-
?
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
involved in oleic acid biosynthesis
-
-
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
involved in oleic acid biosynthesis
-
-
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-, P22243
enzyme catalyzes the principal conversion of saturated fatty acids to unsaturated fatty acids in synthesis of vegetable oils
-
?
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + electron acceptor + H2O
show the reaction diagram
O22832, Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
preferred substrate for S-ACP-DES1, S-ACP-DES1 preferentially desaturates the substrate at C9 position
-
-
?
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + electron acceptor + H2O
show the reaction diagram
O22832, Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
preferred substrate for S-ACP-DES5, S-ACP-DES5 preferentially desaturates the substrate at C9 position
-
-
?
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + electron acceptor + H2O
show the reaction diagram
O22832, Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
S-ACP-DES3 preferentially desaturates the substrate at C9 position
-
-
?
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + electron acceptor + H2O
show the reaction diagram
O22832, Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
very low activity of S-ACP-DES4, S-ACP-DES4 preferentially desaturates the substrate at C9 position
-
-
?
stearoyl-[acyl-carrier protein] + ferredoxin + O2
oleoyl-[acyl-carrier protein] + oxidized ferredoxin + H2O
show the reaction diagram
-, Q4JIJ4
-
-
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
-
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
-
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
no activity with NADH
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
P22243
no activity with NADH
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
electron donor NADPH
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
electron donor NADPH
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
electron donor NADPH
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
P22243
electron donor NADPH
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
purified enzyme is not active with NADH
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
P22243
a system composed of ferredoxin, grana lamellae, ascorbic acid, dichlorophenolindophenol and light is the most effective reductant
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
enzyme acts on derivatives of a number of long chain fatty acids
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
electron donor NADPH-ferredoxin(II)
-
-
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
enzyme is specific for stearoyl-CoA
-
-
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
P22243
enzyme is specific for stearoyl-CoA
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
6-octadecenoyl-[acyl-carrier protein] + 9-octadecenoyl-[acyl-carrier protein]acceptor + H2O
show the reaction diagram
-
-
ratio of activity: 2/1
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
9-octadecenoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
O24498
-
-
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
9-octadecenoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
stearoyl-substrate is about 100fold preferred over palmitoyl-substrate
-
-
?
stearoyl-[acyl-carrier-protein] + reduced acceptor + O2
oleoyl-[acyl-carrier-protein] + acceptor + H2O
show the reaction diagram
-
-
-
-
?
stearoyl-[acyl-carrier-protein] + reduced acceptor + O2
oleoyl-[acyl-carrier-protein] + acceptor + H2O
show the reaction diagram
A8D2K7
-
-
-
?
tetradecanoyl-[acyl-carrier protein] + reduced acceptor + O2
9-tetradecenoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
-
-
-
-
tetradecanoyl-[acyl-carrier protein] + reduced acceptor + O2
9-tetradecenoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
3% of activity with stearoyl-[acyl-carrier-protein], 30fold higher activity than castor enzyme
-
?
tetradecanoyl-[acyl-carrier protein] + reduced acceptor + O2
9-tetradecenoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
3fold more active as with palmitoyl-[acyl-carrier protein], very low activity with dodecanoyl-[acyl-carrier protein] and stearoyl-[acyl-carrier protein]
-
?
heptadecanoyl-[acyl-carrier protein] + reduced ferredoxin + O2
9-heptadecenoyl-[acyl-carrier protein] + oxidized ferredoxin + H2O
show the reaction diagram
-
very low activity, vegetative ferredoxin from Anabaena
-
?
additional information
?
-
-
in vitro analysis of the enzyme does not show any product, lack of soluble OeSAD2 protein expression precludes its in vitro analysis, OsSAD2 is active both on 18:0-[acyl-carrier protein] DELTA9 and 16:0-[acyl-carrier protein] DELTA4 substrates producing 18:1 DELTA9 and 16:1 DELTA4 products
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
involved in oleic acid biosynthesis
-
-
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
involved in oleic acid biosynthesis
-
-
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-, P22243
enzyme catalyzes the principal conversion of saturated fatty acids to unsaturated fatty acids in synthesis of vegetable oils
-
?
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + electron acceptor + H2O
show the reaction diagram
O22832, Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
preferred substrate for S-ACP-DES1, S-ACP-DES1 preferentially desaturates the substrate at C9 position
-
-
?
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + electron acceptor + H2O
show the reaction diagram
O22832, Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
preferred substrate for S-ACP-DES5, S-ACP-DES5 preferentially desaturates the substrate at C9 position
-
-
?
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + electron acceptor + H2O
show the reaction diagram
O22832, Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
S-ACP-DES3 preferentially desaturates the substrate at C9 position
-
-
?
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + electron acceptor + H2O
show the reaction diagram
O22832, Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
very low activity of S-ACP-DES4, S-ACP-DES4 preferentially desaturates the substrate at C9 position
-
-
?
stearoyl-[acyl-carrier protein] + ferredoxin + O2
oleoyl-[acyl-carrier protein] + oxidized ferredoxin + H2O
show the reaction diagram
-, Q4JIJ4
-
-
-
?
stearoyl-[acyl-carrier-protein] + reduced acceptor + O2
oleoyl-[acyl-carrier-protein] + acceptor + H2O
show the reaction diagram
-
-
-
-
?
stearoyl-[acyl-carrier-protein] + reduced acceptor + O2
oleoyl-[acyl-carrier-protein] + acceptor + H2O
show the reaction diagram
A8D2K7
-
-
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Ferredoxin
P22337
required for activity
-
Ferredoxin
-
required for activity
-
Ferredoxin
-
stearyl acyl carrier protein desaturase system consists of 3 components: a reduced NADPH-oxidoreductase, a desaturase and ferredoxin
-
Ferredoxin
-
-
-
Ferredoxin
-
-
-
Ferredoxin
-
-
-
Ferredoxin
-
-
-
Ferredoxin
Bignonia unguis-cati
P92933
-
-
Ferredoxin
-, Q9XFH1
;
-
Ferredoxin
-
highest activity with Anabaena ferredoxin
-
Ferredoxin
-
highest activity with Anabaena or impatiens ferredoxin
-
Ferredoxin
-
-
-
Ferredoxin
-
highest activity with Anabaena ferredoxin
-
Ferredoxin
-
-
-
Ferredoxin
-, Q4JIJ4
reduced spinach ferredoxin
-
additional information
P22243
NADH is ineffective as electron donor
-
additional information
-
NADH is ineffective as electron donor
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Iron
-
3.85 mol of iron per mol of holoenzyme, dimeric enzyme may contain a pair of identical diiron-oxo clusters
Iron
-
di-iron center
additional information
O53442, Q50824
absence of iron in both the native and selenomethionine-substituted crystals, X-ray fluorescence scan
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
beta-mercaptoethanol
P22243
-
cytochrome c3
P22243
-
-
cytochrome c553
P22243
-
-
FAD
-
0.005 mM, 74% inhibition
FMN
-
0.006 mM, 73% inhibition
KCN
P22243
1 mM, strong inhibition
KCN
-
0.01 mM, 50% inhibition, 0.1 mM, 80% inhibition
additional information
P22243
not inhibited by CO
-
additional information
-
not inhibited by CO
-
additional information
Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
inhibition of activity of 27% for S-ACP-DES5 in the presence of 5fold excess of unlabeled product oleoyl-[acyl-carrier protein]; inhibition of activity of 50% for S-ACP-DES4 in the presence of 5fold excess of unlabeled product oleoyl-[acyl-carrier protein]; inhibition of activity of 67% for S-ACP-DES3 in the presence of 5fold excess of unlabeled product oleoyl-[acyl-carrier protein] and 40% for S-ACP-DES3 in the presence of 5fold unlabeled product palmitoleoyl-[acyl-carrier protein], respectively; inhibition of activity of 73% for SSI2 in the presence of 5fold excess of unlabeled product oleoyl-[acyl-carrier protein] and 37% for SSI2 in the presence of 5fold unlabeled product palmitoleoyl-[acyl-carrier protein], respectively; inhibition of activity of 7% for S-ACP-DES1 in the presence of 5fold excess of unlabeled product oleoyl-[acyl-carrier protein]
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
catalase
-
5-10fold stimulation
-
catalase
-
3fold stimulation of purified enzyme, 4-5fold increase of activity in crude extracts
-
catalase
-
-
-
dithiothreitol
P22243
enhances activity
reduced glutathione
P22243
enhances activity
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0074
-
10-(heptyloxy)-decanoyl-[acyl-carrier protein]
-
-
0.0066
-
7-(decyloxy)-heptanoyl-[acyl-carrier protein]
-
-
0.0059
-
heptadecanoyl-[acyl-carrier protein]
-
-
0.00055
-
hexadecanoyl-[acyl-carrier protein]
-
T117R/G188L mutant enzyme
0.005
-
hexadecanoyl-[acyl-carrier protein]
-
-
0.0083
-
hexadecanoyl-[acyl-carrier protein]
-
-
0.002
-
NADPH
P22243
-
0.0013
-
nonadecanoyl-[acyl-carrier protein]
-
-
0.00046
-
octadecanoyl-[acyl-carrier protein]
-
-
0.00098
-
octadecanoyl-[acyl-carrier protein]
-
T117R/G188L mutant enzyme
0.0033
-
octadecanoyl-[acyl-carrier protein]
-
-
0.0039
-
octadecanoyl-[acyl-carrier protein]
-
-
0.00051
-
palmitoyl-[acyl-carrier protein]
-
-
0.069
-
pentadecanoyl-[acyl-carrier protein]
-
-
0.0083
-
stearoyl-CoA
-
-
0.00038
-
stearoyl-[acyl-carrier protein]
-
-
0.001
-
tetradecanoyl-[acyl-carrier protein]
-
T117R/G188L mutant enzyme
0.0039
-
tetradecanoyl-[acyl-carrier protein]
-
spinach acyl-carrier protein
0.00464
-
tetradecanoyl-[acyl-carrier protein]
-
-
0.0058
-
tetradecanoyl-[acyl-carrier protein]
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.5
-
10-(heptyloxy)-decanoyl-[acyl-carrier protein]
-
-
0.2
-
7-(decyloxy)-heptanoyl-[acyl-carrier protein]
-
-
0.04
-
8-(nonyloxy)-octanoyl-[acyl-carrier protein]
-
-
0.02
-
9-(octyloxy)-nonanoyl-[acyl-carrier protein]
-
-
0.233
-
heptadecanoyl-[acyl-carrier protein]
-
-
0.0467
-
hexadecanoyl-[acyl-carrier protein]
-
-
0.095
-
hexadecanoyl-[acyl-carrier protein]
-
-
0.422
-
hexadecanoyl-[acyl-carrier protein]
-
T117R/G188L mutant enzyme
0.2
-
nonadecanoyl-[acyl-carrier protein]
-
-
0.0817
-
octadecanoyl-[acyl-carrier protein]
-
T117R/G188L mutant enzyme
0.55
-
octadecanoyl-[acyl-carrier protein]
-
-
0.705
-
octadecanoyl-[acyl-carrier protein]
-
-
0.817
-
octadecanoyl-[acyl-carrier protein]
-
-
0.0317
-
pentadecanoyl-[acyl-carrier protein]
-
-
0.333
0.5
stearoyl-[acyl-carrier protein]
-
-
0.0065
-
tetradecanoyl-[acyl-carrier protein]
-
-
0.00717
-
tetradecanoyl-[acyl-carrier protein]
-
-
0.0273
-
tetradecanoyl-[acyl-carrier protein]
-
T117R/G188L mutant enzyme
0.817
-
tetradecanoyl-[acyl-carrier protein]
-
spinach acyl-carrier protein
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.0000016
-
A8D2K7
activity in extracts from Escherichia coli M15 transformed with pQESAD, uninduced, with reduced spinach ferredoxin
0.000002
-
A8D2K7
activity in extracts from Escherichia coli M15 transformed with pQESAD, uninduced, without reduced spinach ferredoxin
0.000003
-
-, Q4JIJ4
His-tag fusion protein overexpressed in Escherichia coli M15 without ferredoxin, without IPTG-induction
0.0000075
-
A8D2K7
activity in extracts from Escherichia coli M15 transformed with pQESAD, induced, with reduced spinach ferredoxin
0.000079
-
A8D2K7
activity in extracts from Escherichia coli M15 transformed with pQESAD, induced, without reduced spinach ferredoxin
0.000082
-
-, Q4JIJ4
His-tag fusion protein overexpressed in Escherichia coli M15 without ferredoxin, IPTG-induced
0.00157
-
-, Q4JIJ4
His-tag fusion protein overexpressed in Escherichia coli M15 with exogenous spinach ferredoxin, without IPTG-induction
0.00795
-
-, Q4JIJ4
His-tag fusion protein overexpressed in Escherichia coli M15 with exogenous spinach ferredoxin, without IPTG-induced
0.065
-
-
-
0.1
-
-
substrate palmitoyl-[acyl-carrier protein]
additional information
-
-
assay procedure
additional information
-
Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
S-ACP-DES1 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]; S-ACP-DES3 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]; S-ACP-DES4 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]; S-ACP-DES5 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
-
A8D2K7
activity assay
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
7.7
-
pH 5.5: about 50% activity, pH 7.7: about 50% activity
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
22
-
-
assay at
23
-
A8D2K7
activity assay
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.73
-
A8D2K7
theoretical
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
RT-PCR, gene-specific primers for the isoform; RT-PCR, gene-specific primers for the isoform; RT-PCR, gene-specific primers for the isoform; RT-PCR, gene-specific primers for the isoform; RT-PCR, gene-specific primers for the isoform; RT-PCR, gene-specific primers for the isoform
Manually annotated by BRENDA team
-, Q4JIJ4
highly expressed in developing fruits
Manually annotated by BRENDA team
-, Q4JIJ4
young
Manually annotated by BRENDA team
-
49000 Da isoform
Manually annotated by BRENDA team
-, Q4JIJ4
young and mature
Manually annotated by BRENDA team
Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
Northern blot, RT-PCR, gene-specific primers for the isoform; Northern blot, RT-PCR, gene-specific primers for the isoform; Northern blot, RT-PCR, gene-specific primers for the seven isoforms; Northern blot, RT-PCR, gene-specific primers for the seven isoforms; Northern blot, RT-PCR, gene-specific primers for the seven isoforms; Northern blot, RT-PCR, gene-specific primers for the seven isoforms
Manually annotated by BRENDA team
-
flax ovary, RT-PCR
Manually annotated by BRENDA team
-, Q4JIJ4
young
Manually annotated by BRENDA team
Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
RT-PCR, gene-specific primers for the isoform; RT-PCR, gene-specific primers for the isoform; RT-PCR, gene-specific primers for the isoform; RT-PCR, gene-specific primers for the isoform; RT-PCR, gene-specific primers for the isoform; RT-PCR, gene-specific primers for the isoform
Manually annotated by BRENDA team
-
found in developing seeds, 15-50 days after flowering, not in germinated seeds
Manually annotated by BRENDA team
Bignonia unguis-cati
P92933
-
Manually annotated by BRENDA team
-
2 isoforms
Manually annotated by BRENDA team
-, Q4JIJ4
young
Manually annotated by BRENDA team
Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
RT-PCR, gene-specific primers for the isoform; RT-PCR, gene-specific primers for the isoform; RT-PCR, gene-specific primers for the isoform; RT-PCR, gene-specific primers for the isoform; RT-PCR, gene-specific primers for the isoform; RT-PCR, gene-specific primers for the isoform
Manually annotated by BRENDA team
-, Q4JIJ4
young
Manually annotated by BRENDA team
Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
RT-PCR, gene-specific primers for the isoform; RT-PCR, gene-specific primers for the isoform; RT-PCR, gene-specific primers for the isoform; RT-PCR, gene-specific primers for the isoform; RT-PCR, gene-specific primers for the isoform; RT-PCR, gene-specific primers for the isoform
Manually annotated by BRENDA team
additional information
-
bolls at different developmental stages, RT-PCR
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
predicted; predicted; predicted; predicted; predicted; predicted; predicted
Manually annotated by BRENDA team
Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
predicted
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
41800
-
A8D2K7
theoretical
43000
-
-, Q4JIJ4
SDS-PAGE, overexpressed His-tag fusion protein
43500
-
A8D2K7
recombinant 6 x His-SAD, determined by SDS-PAGE
44180
-
Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
calculated from amino acid sequence
45020
-
Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
calculated from amino acid sequence
45290
-
Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
calculated from amino acid sequence
45480
-
Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
calculated from amino acid sequence
45510
-
-, Q4JIJ4
precursor
45690
-
Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
calculated from amino acid sequence
45860
-
Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
calculated from amino acid sequence
46950
-
Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
calculated from amino acid sequence
50500
-
-
determined by SDS-PAGE
68000
-
-
gel filtration
75000
-
-
recombinant enzyme lacking a putative transit peptide
additional information
-
O53442, Q50824
unlabeled, purified DesA2 protein, MALDI-TOF, 31138 and 31345 m/z
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 39000, recombinant enzyme, SDS-PAGE, predicted from nucleotide sequence
?
-
x * 45000, isoform 1, SDS-PAGE; x * 49000, isoform 2, SDS-PAGE
?
O24498
x * 45000, SDS-PAGE, recombinant enzyme with His-tag
dimer
-
2 * 36000, SDS-PAGE
dimer
-
alpha2, 2 * 37000, recombinant enzyme lacking a putative transit peptide, SDS-PAGE
dimer
O53442, Q50824
DesA2 isoform, gel filtration
dimer
Mycobacterium tuberculosis H37Rv,variant DesA2
-
DesA2 isoform, gel filtration
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
DesA2 crystals grown by the method of hanging drop vapor diffusion, one metal bound per subunit which is likely Mn2+, native and selenomethionine-substituted, 2.0 A resolution X-ray structure
O53442, Q50824
hanging drop vapor diffusion, 12-18 mg/ml enzyme solution, reservoir solution contains 100 mM cacodylate buffer, pH 5.4, 200 mM magnesium acetate and 12-18% polyethyleneglycol 8000, needle shaped crystals too thin for X-ray study
-
in complex with azide or acetae, apo- and single-iron form
-
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50
-
-
1 min, inactivation
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
unstable to dialysis
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-70C, 50% glycerol, 0.1% bovine serum albumin, 100 mM potassium phosphate buffer, pH 6.8, at least 2 months, no loss of activity
-
4C, overnight, 30% loss of activity
-
0C, 10 h, 50% loss of activity
-
frozen state, 1 month, complete loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
by Ni-NTA purification system
-
overexpressed S-ACP-DES1 enzyme; overexpressed S-ACP-DES3 enzyme; overexpressed S-ACP-DES4 enzyme; overexpressed S-ACP-DES5 enzyme
Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
recombinant enzyme
Bignonia unguis-cati
P92933
aceton powder, anion-exchange, affinity chromatography on acyl-carrier protein-Sepharose
-
by gel filtration
A8D2K7
DesA2 isoform purified by ion exchange and gel filtration chromatographies
O53442, Q50824
recombinant enzyme
-
recombinant enzyme lacking a putative transit peptide
-
recombinant enzyme
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
into the pUCm-T vector and subcloned into the vector pET30a for expression in Escherichia coli BL21DE3 cells
-
S-ACP-DES1 expressed in Escherichia coli; S-ACP-DES3 enzyme expressed in Escherichia coli; S-ACP-DES4 enzyme expressed in Escherichia coli; S-ACP-DES5 enzyme expressed in Escherichia coli
Q6NQK5, Q84VY3, Q8L468, Q9LF04, Q9LF05, Q9M880
expression in Escherichia coli
-
expression of 2 isoforms in Escherichia coli
-
expression in Escherichia coli
Bignonia unguis-cati
P92933
a Brassica napus bacterial artificial chromosome, BAC, library is constructed
-
expression of cDNA in Escherichia coli
-
into the vector pQE-30 for expression in Escherichia coli M15 cells
A8D2K7
full-length cDNA clone
-
cloning of isoform 1; cloning of isoform 2
-, Q9XFH1
cloned in pQE-30 vector and expressed in Escherichia coli strain M15, His-tag
-, Q4JIJ4
expression of cDNA in Escherichia coli
-
DesA1 overexpressed in Escherichia coli BL21-(DE3) with the natural N-terminal methionine residue as the first amino acid; DesA2 overexpressed in Escherichia coli BL21-(DE3) with the natural N-terminal methionine residue as the first amino acid
O53442, Q50824
expression of cDNA in Escherichia coli
-
OeSAD2 is not expressed as a soluble protein
-
expression of cDNA in Escherichia coli
-
expression of N-terminal truncated enzyme in Escherichia coli
-
expression of wild-type, A181T/A188G/Y189F/S205N/L206T/G207A, A188G/Y189F, A181T/A200F and A181T/A200F/S205N/L206T/G207A mutant enzyme in Escherichia coli
-
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ahSAD3 is expressed exclusively during seed development
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
L118F/P179I
-
15fold higher activity with palmitoyl-[acyl-carrier protein] than wild-type enzyme, low DELTA10 desaturase activity
L118W
-
100% activity with palmitoyl-[acyl-carrier protein], 89% activity with stearoyl-[acyl-carrier protein] and 8.2% activity with myristoyl-[acyl-carrier protein], wild-type is only active with stearoyl-[acyl-carrier protein]
T117R/G188L
-
82fold higher specificity for palmitoyl-[acyl-carrier protein] with respect to wild-type
A181T/A188G/Y189F/S205N/L206T/G207A
-
compared to wild-type: reduced DELTA6 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate, strong DELTA9 desaturase activity with stearoyl-[acyl-carrier protein] as substrate, exhibits DELTA9 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate
A181T/A200F
-
increase in DELTA6 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate, strong DELTA9 desaturase activity with stearoyl-[acyl-carrier protein] as substrate
A181T/A200F/S205N/L206T/G207A
-
reduced DELTA6 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate, very low DELTA9 desaturase activity, no DELTA6 desaturase activity with stearoyl-[acyl-carrier protein] as substrate
A188G/Y189F
-
reduced DELTA6 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
agriculture
-
mutant strain showing contents of up to 20% of unusual acyl chains such as 16:1DELTA9, 16:2DDELTA9,12, 18:1DELTA11, increase of enzyme activity in mutant