Information on EC 1.14.17.1 - dopamine beta-monooxygenase

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The expected taxonomic range for this enzyme is: Coelomata

EC NUMBER
COMMENTARY
1.14.17.1
-
RECOMMENDED NAME
GeneOntology No.
dopamine beta-monooxygenase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
dopamine + ascorbate + O2 = noradrenaline + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
dopamine + ascorbate + O2 = noradrenaline + dehydroascorbate + H2O
show the reaction diagram
new mechanism of enzyme
-
dopamine + ascorbate + O2 = noradrenaline + dehydroascorbate + H2O
show the reaction diagram
uni-uni bi-uni ping pong mechanism
-
dopamine + ascorbate + O2 = noradrenaline + dehydroascorbate + H2O
show the reaction diagram
ping pong mechanism
-
dopamine + ascorbate + O2 = noradrenaline + dehydroascorbate + H2O
show the reaction diagram
stoichiometry
-
dopamine + ascorbate + O2 = noradrenaline + dehydroascorbate + H2O
show the reaction diagram
mechanism; stoichiometry
-
dopamine + ascorbate + O2 = noradrenaline + dehydroascorbate + H2O
show the reaction diagram
mechanism
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
catecholamine biosynthesis
-
Metabolic pathways
-
Tyrosine metabolism
-
SYSTEMATIC NAME
IUBMB Comments
3,4-dihydroxyphenethylamine,ascorbate:oxygen oxidoreductase (beta-hydroxylating)
A copper protein. Stimulated by fumarate.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3,4-dihydroxyphenethylamine beta-oxidase
-
-
-
-
3,4-dihydroxyphenylethylamine beta-hydoxylase
-
-
-
-
4-(2-aminoethyl)pyrocatechol beta-oxidase
-
-
-
-
DbetaH
-
-
DbetaM
-
-
Dopa beta-hydroxylase
-
-
-
-
dopamine beta hydroxylase
E9JGS5
-
dopamine beta-hydrolase
-
-
-
-
dopamine beta-hydroxylase
-
-
-
-
dopamine beta-hydroxylase
-
-
dopamine beta-hydroxylase
-
-
dopamine beta-hydroxylase
-
-
dopamine beta-hydroxylase
-
-
dopamine beta-hydroxylase
-
-
dopamine beta-hydroxylase
-
-
dopamine beta-mono-oxygenase
-
-
dopamine beta-monooxygenase
-
-
dopamine beta-oxidase
-
-
-
-
dopamine hydroxylase
-
-
-
-
dopamine(3,4-dihydroxyphenethylamine)beta-mono-oxygenase
-
-
-
-
dopamine-B-hydroxylase
-
DBH
dopamine-beta hydroxylase
-
-
dopamine-beta-hydroxylase
-
-
dopamine-beta-hydroxylase
-
-
dopamine-beta-hydroxylase
-
-
dopamine-beta-monooxygenase
-
-
EC 1.14.2.1
-
-
formerly
-
MDBH
-
membrane-associated dopamine beta-monooxygenase
oxygenase, dopamine beta-mono-
-
-
-
-
pDbetaH
-
-
phenylamine beta-hydroxylase
-
-
-
-
plasma DbetaH activity
-
-
plasma dopamine beta-hydroxylase
-
-
plDbetaH
-
-
SDBH
-
soluble dopamine beta-monooxygenase
tyramine beta-monooxygenase
-
-
CAS REGISTRY NUMBER
COMMENTARY
9013-38-1
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
ascorbate
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
9.5
-
1-(4-Hydroxybenzyl)imidazole
-
-
10
-
1-Phenyl-1-aminomethylethene
-
-
65
-
1-Phenylethylamine
-
-
56
-
2-(4-hydroxphenyl)prop-2-enylamine
-
-
0.223
-
2-Bromo-3-(p-hydroxyphenyl)-1-propene
-
-
1.1
-
2-chlorophenethylamine
-
-
4.2
-
2-hydroxyphenethylamine
-
-
14
-
2-Phenylprop-2-enylamine
-
-
12
-
3-Phenylpropylamine
-
-
20.4
-
3-Phenylpropylamine
-
-
0.7
-
4-Hydroxy-alpha-methylstyrene
-
-
13
-
dopamine
-
-
110
-
dopamine
-
-
21
-
N-Phenylethylenediamine
-
-
33
-
Octopamine
-
-
1.7
-
Phenylacetaldehyde
-
-
39
-
Phenylaminoethyl sulfide
-
-
-
68
-
Phenylaminoethyl sulfide
-
-
-
121
-
tyramine
-
-
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.55
-
ascorbate
-
at 0.7 mM tyramine, pH 6 and 35C
7212
0.8
-
ascorbate
-
at 0.3 mM tyramine, pH 6 and 35C
7212
1.14
-
ascorbate
-
at 0.1 mM tyramine, pH 6 and 35C
7212
1.38
-
ascorbate
-
at 0.05 mM tyramine, pH 6 and 35C
7212
4.95
-
beta,beta-dideuterated tyramine
-
at 10 mM ascorbate, 0.056 mM O2, pH 6 and 35C
293053
5.8
-
beta,beta-dideuterated tyramine
-
at 50 mM ascorbate, 0.063 mM O2, pH 6 and 35C
293053
9.6
-
beta,beta-dideuterated tyramine
-
at 50 mM ascorbate, 0.110 mM O2, pH 6 and 35C
293053
10.9
-
beta,beta-dideuterated tyramine
-
at 10 mM ascorbate, 0.119 mM O2, pH 6 and 35C
293053
13.5
-
beta,beta-dideuterated tyramine
-
at 50 mM ascorbate, 0.214 mM O2, pH 6 and 35C
293053
14.3
-
beta,beta-dideuterated tyramine
-
at 10 mM ascorbate, 0.215 mM O2, pH 6 and 35C
293053
19.4
-
beta,beta-dideuterated tyramine
-
at 10 mM ascorbate, 0.395 mM O2, pH 6 and 35C; at 50 mM ascorbate, 0.416 mM O2, pH 6 and 35C
293053
20.1
-
beta,beta-dideuterated tyramine
-
at 10 mM ascorbate, 0.587 mM O2, pH 6 and 35C
293053
24.6
-
beta,beta-dideuterated tyramine
-
at 50 mM ascorbate, 0.598 mM O2, pH 6 and 35C
293053
29.2
-
beta,beta-dideuterated tyramine
-
at 50 mM ascorbate, 0.907 mM O2, pH 6 and 35C
293053
38.6
-
beta,beta-dideuterated tyramine
-
at 50 mM ascorbate, 1.039 mM O2, pH 6 and 35C
293053
39.4
-
beta,beta-dideuterated tyramine
-
at 10 mM ascorbate, 0.907 mM O2, pH 6 and 35C
293053
15.4
-
tyramine
-
at 50 mM ascorbate, 0.059 mM O2, pH 6 and 35C
17592
20.7
-
tyramine
-
at 10 mM ascorbate, 0.058 mM O2, pH 6 and 35C
17592
25.5
-
tyramine
-
at 10 mM ascorbate, 0.103 mM O2, pH 6 and 35C
17592
27.2
-
tyramine
-
at 50 mM ascorbate, 0.099 mM O2, pH 6 and 35C
17592
31.9
-
tyramine
-
at 200 mM ascorbate, pH 6 and 35C
17592
32.4
-
tyramine
-
at 100 mM ascorbate, pH 6 and 35C
17592
33
-
tyramine
-
at 4 mM ascorbate, pH 6 and 35C
17592
37.8
-
tyramine
-
at 50 mM ascorbate, 0.214 mM O2, pH 6 and 35C; at 50 mM ascorbate, pH 6 and 35C
17592
44.7
-
tyramine
-
at 10 mM ascorbate, 0.212 mM O2, pH 6 and 35C
17592
45
-
tyramine
-
at 10 mM ascorbate, pH 6 and 35C
17592
47.7
-
tyramine
-
at 50 mM ascorbate, 0.395 mM O2, pH 6 and 35C
17592
48.2
-
tyramine
-
at 50 mM ascorbate, 0.854 mM O2, pH 6 and 35C
17592
52
-
tyramine
-
at 2 mM ascorbate, pH 6 and 35C
17592
58.1
-
tyramine
-
at 10 mM ascorbate, 0.512 mM O2, pH 6 and 35C
17592
58.6
-
tyramine
-
at 50 mM ascorbate, 0.608 mM O2, pH 6 and 35C
17592
61.7
-
tyramine
-
at 10 mM ascorbate, 0.885 mM O2, pH 6 and 35C
17592
70
-
tyramine
-
at 50 mM ascorbate, 1.04 mM O2, pH 6 and 35C
17592
74.3
-
tyramine
-
at 10 mM ascorbate, 1.04 mM O2, pH 6 and 35C
17592
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0057
-
1(2H)-Phthalazine hydrazone
-
-
4.6
-
1-(3,4-Dihydroxybenzyl)imidazole
-
-
2.9
-
1-(4-Hydroxybenzyl)-2-methylimidazole
-
-
0.16
-
1-(4-Hydroxybenzyl)pyrazole
-
-
11.9
-
1-Benzylimidazole
-
-
0.0009
-
1-Isoquinolinecarboxylic acid
-
-
0.8
-
1-Isoquinolinecarboxylic acid
-
-
13
-
1-Phenyl-1-aminomethylethene
-
-
2.5
-
1-Phenylpropene
-
-
0.0019
-
2(1H)-Pyridinone hydrazone
-
-
0.021
-
2,2'-Bi-(1H)-imidazole
-
-
0.52
-
2-(4-Hydroxyphenyl)prop-2-enylamine
-
-
4.9
-
2-Bromo-3-(p-hydroxyphenyl)-1-propene
-
-
4.7
-
2-Phenylprop-2-enylamine
-
-
3.6
-
3-Phenylpropene
-
-
2.5
-
4-Hydroxy-alpha-methylstyrene
-
-
2.3
-
4-hydroxybenzaldehyde
-
-
0.0011
-
ascorbate
-
at 0.05 mM tyramine, pH 6 and 35C
0.0018
-
ascorbate
-
at 0.1 mM tyramine, pH 6 and 35C
20
-
ascorbate
-
-
1.25
-
beta,beta-dideuterated tyramine
-
at 10 mM ascorbate, 0.587 mM O2, pH 6 and 35C
2
-
beta,beta-dideuterated tyramine
-
at 10 mM ascorbate, 0.907 mM O2, pH 6 and 35C
2.1
-
beta,beta-dideuterated tyramine
-
at 10 mM ascorbate, 0.215 mM O2, pH 6 and 35C
2.4
-
beta,beta-dideuterated tyramine
-
at 10 mM ascorbate, 0.395 mM O2, pH 6 and 35C
2.8
-
beta,beta-dideuterated tyramine
-
at 10 mM ascorbate, 0.119 mM O2, pH 6 and 35C; at 50 mM ascorbate, 0.416 mM O2, pH 6 and 35C
2.86
-
beta,beta-dideuterated tyramine
-
at 50 mM ascorbate, 1.039 mM O2, pH 6 and 35C
3.3
-
beta,beta-dideuterated tyramine
-
at 50 mM ascorbate, 0.598 mM O2, pH 6 and 35C
3.35
-
beta,beta-dideuterated tyramine
-
at 10 mM ascorbate, 0.056 mM O2, pH 6 and 35C
4
-
beta,beta-dideuterated tyramine
-
at 50 mM ascorbate, 0.907 mM O2, pH 6 and 35C
6
-
beta,beta-dideuterated tyramine
-
at 50 mM ascorbate, 0.110 mM O2, pH 6 and 35C
7.4
-
beta,beta-dideuterated tyramine
-
at 50 mM ascorbate, 0.063 mM O2, pH 6 and 35C
7.8
-
beta,beta-dideuterated tyramine
-
at 50 mM ascorbate, 0.214 mM O2, pH 6 and 35C
26
-
beta,beta-difluorophenethylamine
-
pH 6.0, 35C
0.015
-
beta-ethynytyramine
-
R,S-1
-
0.057
-
beta-ethynytyramine
-
S-1, in the presence of ascorbate
-
0.208
-
beta-ethynytyramine
-
S-1
-
0.41
-
histidine
-
-
0.37
-
hydroquinone
-
pH: 4.9
6.8
-
N-ethylaniline
-
-
1.1
-
N-Phenylethylenediamine
-
-
5
-
norepinephrine
-
-
0.14
-
Quinoline-2-carboxylic acid
-
-
0.98
-
tyramine
-
at 10 mM ascorbate, 0.885 mM O2, pH 6 and 35C
1.23
-
tyramine
-
at 10 mM ascorbate, 1.04 mM O2, pH 6 and 35C
1.3
-
tyramine
-
at 4 mM ascorbate, pH 6 and 35C
1.57
-
tyramine
-
at 50 mM ascorbate, 0.854 mM O2, pH 6 and 35C
1.9
-
tyramine
-
at 50 mM ascorbate, 0.608 mM O2, pH 6 and 35C
2.03
-
tyramine
-
at 50 mM ascorbate, 1.04 mM O2, pH 6 and 35C
2.07
-
tyramine
-
at 10 mM ascorbate, 0.512 mM O2, pH 6 and 35C
2.1
-
tyramine
-
at 2 mM ascorbate, pH 6 and 35C
2.46
-
tyramine
-
at 50 mM ascorbate, 0.395 mM O2, pH 6 and 35C
3.33
-
tyramine
-
at 10 mM ascorbate, 0.103 mM O2, pH 6 and 35C
3.5
-
tyramine
-
at 10 mM ascorbate, 0.212 mM O2, pH 6 and 35C; at 10 mM ascorbate, pH 6 and 35C
3.6
-
tyramine
-
at 100 mM ascorbate, pH 6 and 35C
3.97
-
tyramine
-
at 50 mM ascorbate, 0.099 mM O2, pH 6 and 35C
4.15
-
tyramine
-
at 50 mM ascorbate, 0.214 mM O2, pH 6 and 35C; at 50 mM ascorbate, pH 6 and 35C
7.82
-
tyramine
-
at 10 mM ascorbate, 0.058 mM O2, pH 6 and 35C
10.2
-
tyramine
-
at 50 mM ascorbate, 0.059 mM O2, pH 6 and 35C
10.3
-
tyramine
-
at 200 mM ascorbate, pH 6 and 35C
1.05
-
hydroquinone
-
pH: 5.9
additional information
-
additional information
-
-
-
additional information
-
additional information
-
KI as function of pH and fumarate activation
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.5
6.5
-
sharp decrease in activity between pH 4.5 and 5.0 and between pH 6.0 and 6.5
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.23
-
E9JGS5
theoretical
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0
4
-
24 h, 10-15% loss of activity
50
-
-
4 h, about 10% loss of activity
60
-
-
2 h, about 30% loss of activity