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Information on EC 1.14.16.5 - alkylglycerol monooxygenase and Organism(s) Mus musculus

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EC Tree
IUBMB Comments
The enzyme cleaves alkylglycerols, but does not cleave alkenylglycerols (plasmalogens). Requires non-heme iron , reduced glutathione and phospholipids for full activity. The product spontaneously breaks down to form a fatty aldehyde and glycerol. The co-product, 4a-hydroxytetrahydropteridine, is rapidly dehydrated to 6,7-dihydropteridine, either spontaneously or by EC 4.2.1.96, 4a-hydroxytetrahydrobiopterin dehydratase.
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Mus musculus
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
alkylglycerol monooxygenase, glyceryl ether monooxygenase, glyceryl-ether monooxygenase, alkylglycerol mono-oxygenase, o-alkylglycerol monooxygenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alkylglycerol monooxygenase
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-
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glyceryl ether hydroxylase
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-
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glyceryl ether monooxygenase
glyceryl ether oxidase
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-
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glyceryl ether oxygenase
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-
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glyceryl ether-cleaving enzyme
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-
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glyceryl etherase
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-
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glyceryl-ether cleaving enzyme
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-
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O-alkylglycerol monooxygenase
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-
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oxygenase, glyceryl ether mono-
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-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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SYSTEMATIC NAME
IUBMB Comments
1-alkyl-sn-glycerol,tetrahydrobiopteridine:oxygen oxidoreductase
The enzyme cleaves alkylglycerols, but does not cleave alkenylglycerols (plasmalogens). Requires non-heme iron [7], reduced glutathione and phospholipids for full activity. The product spontaneously breaks down to form a fatty aldehyde and glycerol. The co-product, 4a-hydroxytetrahydropteridine, is rapidly dehydrated to 6,7-dihydropteridine, either spontaneously or by EC 4.2.1.96, 4a-hydroxytetrahydrobiopterin dehydratase.
CAS REGISTRY NUMBER
COMMENTARY hide
37256-82-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-alkyl-sn-glycerol + tetrahydrobiopterin + O2
1-O-alkyl-sn-glycerol + dihydrobiopterin + H2O
show the reaction diagram
-
-
-
-
?
1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine + tetrahydrobiopterin + O2
glycerophosphocholine + alkylaldehyde + dihydrobiopterin + H2O
show the reaction diagram
-
1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine is lyso-platelet activating factor
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-
?
1-O-alkyl-sn-glycerol + tetrahydrobiopterin + O2
1-O-(1-hydroxyalkyl)-sn-glycerol + dihydrobiopterin + H2O
show the reaction diagram
-
-
-
-
?
hexadecylglycerol + tetrahydrobiopterin + O2
hexadecanal + glycerol + dihydrobiopterin + H2O
show the reaction diagram
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-
-
-
?
octadecylglycerol + tetrahydrobiopterin + O2
?
show the reaction diagram
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-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1-alkyl-sn-glycerol + tetrahydrobiopterin + O2
1-O-alkyl-sn-glycerol + dihydrobiopterin + H2O
show the reaction diagram
-
-
-
-
?
1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine + tetrahydrobiopterin + O2
glycerophosphocholine + alkylaldehyde + dihydrobiopterin + H2O
show the reaction diagram
-
1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine is lyso-platelet activating factor
-
-
?
1-O-alkyl-sn-glycerol + tetrahydrobiopterin + O2
1-O-(1-hydroxyalkyl)-sn-glycerol + dihydrobiopterin + H2O
show the reaction diagram
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-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
tetrahydrobiopterin
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dependent on
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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the enzyme modulates macrophage macrophage platelet-activating factor production by regulating cellular lyso-platelet-activating factor levels
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ALKMO_MOUSE
447
5
51778
Swiss-Prot
other Location (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48000
-
x * 48000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 48000, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D233A
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inactive
Q197A
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the mutant retains minimal activity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in HEK-293 cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
mRNA expression levels decrease with lipopolysaccharide treatments in mouse peritoneal macrophages and RAW264.7 cells
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Pfleger, R.C.; Piantadosi, C.; Snyder, F.
The biocleavage of isomeric glyceryl ethers by soluble liver enzymes in a variety of species
Biochim. Biophys. Acta
144
633-648
1967
Canis lupus familiaris, Cavia porcellus, Limax maximus, Meriones sp., Mesocricetus auratus, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
Manually annotated by BRENDA team
Taguchi, H.; Armarego, W.L.F.
Glyceryl-ether monooxygenase [EC 1.14.16.5]. A microsomal enzyme of ether lipid metabolism
Med. Res. Rev.
18
43-89
1998
Canis lupus familiaris, Cavia porcellus, Meriones sp., Mesocricetus auratus, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
Manually annotated by BRENDA team
Tokuoka, S.M.; Kita, Y.; Shindou, H.; Shimizu, T.
Alkylglycerol monooxygenase as a potential modulator for PAF synthesis in macrophages
Biochem. Biophys. Res. Commun.
436
306-312
2013
Mus musculus
Manually annotated by BRENDA team
Watschinger, K.; Keller, M.A.; McNeill, E.; Alam, M.T.; Lai, S.; Sailer, S.; Rauch, V.; Patel, J.; Hermetter, A.; Golderer, G.; Geley, S.; Werner-Felmayer, G.; Plumb, R.S.; Astarita, G.; Ralser, M.; Channon, K.M.; Werner, E.R.
Tetrahydrobiopterin and alkylglycerol monooxygenase substantially alter the murine macrophage lipidome
Proc. Natl. Acad. Sci. USA
112
2431-2436
2015
Mus musculus
Manually annotated by BRENDA team