Information on EC 1.14.15.4 - steroid 11beta-monooxygenase

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The expected taxonomic range for this enzyme is: Opisthokonta

EC NUMBER
COMMENTARY
1.14.15.4
-
RECOMMENDED NAME
GeneOntology No.
steroid 11beta-monooxygenase
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydroxylation
-
-
-
-
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
glucocorticoid biosynthesis
-
Metabolic pathways
-
mineralocorticoid biosynthesis
-
Steroid hormone biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
steroid,reduced-adrenal-ferredoxin:oxygen oxidoreductase (11beta-hydroxylating)
A heme-thiolate protein (P-450). Also hydroxylates steroids at the 18-position, and converts 18-hydroxycorticosterone into aldosterone.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
11-hydroxylase
-
-
11 beta-hydroxylase
A1XRK1
-
11-beta hydroxylase
-
-
11-beta-hydroxylase
-
-
11-hydroxylase
-
-
11beta hydroxylase
-
-
11beta-hydroxylase
-
-
11beta-hydroxylase
Absidia caerulea IBL02
-
-
-
11beta-hydroxylase
-
-
11beta-hydroxylase
Q2I128
-
11beta-hydroxylase
-
-
11beta-hydroxylase
-
-
11beta-hydroxylase
-
-
ALDOS
-
-
-
-
Aldosterone synthase
-
-
-
-
Aldosterone synthase
P15538
-
Aldosterone synthase
P19099
-
Aldosterone synthase
-
-
Aldosterone-synthesizing enzyme
-
-
-
-
C450XIB2
-
-
-
-
CYP11B1
P15538
-
CYP11B1
-
member of the cytochrome p-450 superfamily
CYP11B2
P15538
-
CYP11B2
P19099
-
cyp11beta
A1XRK1
-
CYPXIB
-
-
-
-
CYPXIB1
-
-
-
-
CYPXIB2
-
-
-
-
CYPXIB3
-
-
-
-
cytochrome 11beta-hydroxylase
-
-
cytochrome P-45011-beta
-
-
cytochrome P450 11B1
P15538
-
cytochrome P450 11B2
P19099
-
cytochrome P45011beta
-
-
cytochrome P45011beta-hydroxylase
Q2I128
-
EC 1.14.1.6
-
-
formerly
-
EC 1.99.1.7
-
-
formerly
-
P-450(11 beta,aldo)
-
-
-
-
P-450Aldo
-
-
-
-
P-450c11
-
-
-
-
P-450C18
-
-
-
-
P-450XIB1
-
-
P450 11beta-hydroxylase
-
-
P450(11 beta)-DS
-
-
-
-
P45011beta
-
-
P45011beta
A1XRK1
-
P45011beta
Q2I128
-
P450C11
-
-
-
-
Steroid 11-beta-hydroxylase
-
-
-
-
Steroid 11-beta-hydroxylase
P15538
-
steroid 11-hydroxylase
-
-
steroid 11beta-hydroxylase
-
-
-
-
steroid 11beta-hydroxylase
-
-
steroid 11beta-monooxygenase
-
-
-
-
steroid 11beta/18-hydroxylase
-
-
-
-
Steroid 18-hydroxylase
-
-
-
-
steroid-11beta-hydroxylase
-
-
CAS REGISTRY NUMBER
COMMENTARY
9029-66-7
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain IBL02
-
-
Manually annotated by BRENDA team
Absidia caerulea IBL02
strain IBL02
-
-
Manually annotated by BRENDA team
calf
-
-
Manually annotated by BRENDA team
Boedijn NRRL 2380
-
-
Manually annotated by BRENDA team
gene cyp11b, a protogynous hermaphroditic fish
UniProt
Manually annotated by BRENDA team
gene CYP11B
-
-
Manually annotated by BRENDA team
573 members of 105 British Caucasian families, genes CYP11B1 and CYP11B2
-
-
Manually annotated by BRENDA team
adult Danish Caucasian Type 1 diabetic patients with diabetic nephropathy
-
-
Manually annotated by BRENDA team
British hypertensive patients
-
-
Manually annotated by BRENDA team
cytochrome P450 11B1, mitochondrial precursor
SwissProt
Manually annotated by BRENDA team
gene CYP11B1
-
-
Manually annotated by BRENDA team
gene CYP11B1, a consanguineous Turkish family
-
-
Manually annotated by BRENDA team
Moroccan Jews
-
-
Manually annotated by BRENDA team
sole protogynous freshwater fish found that exhibits an adult sex change from functional females to males
UniProt
Manually annotated by BRENDA team
male Wistar
-
-
Manually annotated by BRENDA team
male Wistar Kyoto rats
-
-
Manually annotated by BRENDA team
male Wistar rats with heart failure induced by coronary artery ligation and sham-operated controls, stroke-prone spontaneously hypertensive rats and Wistar Kyoto rats as controls, cyp1a1Ren-2 transgenic rats and Fischer controls, and adult Sprague-Dawley rats
-
-
Manually annotated by BRENDA team
male, Sprague-Dawley, low sodium, high potassium treated
-
-
Manually annotated by BRENDA team
male, Zur:SIV low sodium, high potassium or high sodium, low potassium treated for stimulation or supprssion, respectively
-
-
Manually annotated by BRENDA team
Milan hypertensive and normotensive rats
-
-
Manually annotated by BRENDA team
neonatal and adult
-
-
Manually annotated by BRENDA team
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
7.2
-
-
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2
15
-
2fold decrease of activity with increase of temperature, temperatures above 25C unfavourable
2
50
-
at 50C almost no activity
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
185000
-
-
exclusion chromatography
1000000
-
-
glycerol density gradient
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2
-
-
crude extract, 7 mg/ml, 24 h, loss of half the activity, DEAE fraction unstable
2
-
-
14% residual activity of 11beta- and 18-hydroxylation
30
-
-
after preincubation for 20 min, 27% residual activity, after 60 min 5.7%
30
-
-
in presence of phospholipids, catalytic activity stable to extended incubation and increased resistance of hemoprotein to denaturation
30
-
-
30 min, 30 mM sodium phosphate buffer, pH 8.0, decrease to 30%, improved to 45-70% by addition of 1 mM GSH, 0.2 mM 11-deoxycortisol, 0.5 mM PMSF or 20% glycerol
30
-
-
purified enzyme, loss of 75% activity after 3 h, and of all activity after 8 h
50
-
-
complete inactivation
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
deoxycorticosterone stabilizes
-
deoxycorticosterone, protection of enzyme from inactivation by 18-ethynylprogesterone
-
inclusion into phospholipid vesicles stabilizes
-
phospholipid vesicles, 0.2% soybean lecithin, added to purified P-45011beta for storage and during incubation
-
11-deoxycortisol, partially stabilizes
-
alpha-lipoic acid, during DEAE chromatography stabilizes
-
EDTA, stabilizes during purification
-
glycerol, partially stabilizes
-
GSH, partially stabilizes
-
GSH, stabilizes during purification, as well as other sulfhydryl compounds
-
PMSF, partially stabilizes
-
Tween 80, positive influence on activity conservation during extraction and fractionation
-