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Information on EC 1.14.14.26 - 24-hydroxycholesterol 7alpha-hydroxylase and Organism(s) Homo sapiens

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IUBMB Comments
A P-450 heme-thiolate protein that is found in liver microsomes and in ciliary non-pigmented epithelium . The enzyme is specific for (24S)-cholest-5-ene-3beta,24-diol, which is formed mostly in the brain by EC 1.14.14.25, cholesterol 24-hydroxylase. The direct electron donor to the enzyme is EC 1.6.2.4, NADPH---hemoprotein reductase.
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Homo sapiens
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The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Synonyms
cyp39a1, cyp39a1 oxysterol 7alpha-hydroxylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
24-hydroxycholesterol 7alpha-monooxygenase
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Cyp39a1
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CYP39A1 oxysterol 7alpha-hydroxylase
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PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
(24R)-cholest-5-ene-3beta,24-diol,NADPH:oxygen oxidoreductase (7alpha-hydroxylating)
A P-450 heme-thiolate protein that is found in liver microsomes and in ciliary non-pigmented epithelium [2]. The enzyme is specific for (24S)-cholest-5-ene-3beta,24-diol, which is formed mostly in the brain by EC 1.14.14.25, cholesterol 24-hydroxylase. The direct electron donor to the enzyme is EC 1.6.2.4, NADPH---hemoprotein reductase.
CAS REGISTRY NUMBER
COMMENTARY hide
149316-80-3
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288309-90-0
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(24R)-cholest-5-ene-3beta,24-diol + NADPH + H+ + O2
(24R)-cholest-5-ene-3beta,7alpha,24-triol + NADP+ + H2O
show the reaction diagram
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activity is largely restricted to 24-hydroxycholesterol
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-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CP7A1_HUMAN
504
1
57661
Swiss-Prot
Secretory Pathway (Reliability: 1)
CP7B1_HUMAN
506
2
58256
Swiss-Prot
Secretory Pathway (Reliability: 4)
CP39A_HUMAN
469
2
54116
Swiss-Prot
Secretory Pathway (Reliability: 1)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Li-Hawkins, J.; Lund, E.G.; Bronson, A.D.; Russell, D.W.
Expression cloning of an oxysterol 7alpha-hydroxylase selective for 24-hydroxycholesterol
J. Biol. Chem.
275
16543-16549
2000
Homo sapiens, Mus musculus (Q9JKJ9), Mus musculus
Manually annotated by BRENDA team