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2,3-dichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
2,3-dichlorophenol + NADPH + O2
3,4-dichlorocatechol + NADP+ + H2O
Soil bacterium
-
113% of the activity with 2,4-dichlorophenol
-
-
?
2,3-dichlorophenol + NADPH + O2
?
2,4,5-trichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
-
low activity
-
-
?
2,4,5-trichlorophenol + NADPH + O2
?
2,4,6-trichlorophenol + NADPH + O2
?
-
-
-
-
?
2,4-dibromophenol + NADPH + O2
?
2,4-dichlorophenol + NADH + H+ + O2
3,5-dichlorocatechol + NAD+ + H2O
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
2,5-dichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
-
-
-
-
?
2,5-dichlorophenol + NADPH + O2
3,6-dichlorocatechol + NADP+ + H2O
Soil bacterium
-
103% of the activity with 2,4-dichlorophenol
-
-
?
2,5-dichlorophenol + NADPH + O2
?
-
-
-
-
?
2,5-dichlorophenol + NADPH + O2
? + NADP+ + H2O
Soil bacterium
-
178% of the activity with 2,4-dichlorophenol
-
-
?
2,6-dichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
-
-
-
-
?
2,6-dichlorophenol + NADPH + O2
?
-
-
-
-
?
2-bromo-4-chlorophenol + NADPH + O2
3-bromo-5-chlorocatechol + NADP+ + H2O
Soil bacterium
-
82% of the activity with 2,4-dichlorophenol
-
-
?
2-chloro-4-nitrophenol + NADPH + O2
?
-
-
-
-
?
2-chlorophenol + NADPH + O2
3-chlorocatechol + NADP+ + H2O
Soil bacterium
-
129% of the activity with 2,4-dichlorophenol
-
-
?
2-chlorophenol + NADPH + O2
?
2-cresol + NADPH + O2
?
-
incompletely metabolized
-
-
?
2-methylphenol + NADPH + O2
?
-
-
-
-
?
3,4-dichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
3,4-dichlorophenol + NADPH + O2
?
-
-
-
-
?
3,5-dichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
-
low activity
-
-
?
3,5-dichlorophenol + NADPH + O2
?
-
-
-
-
?
3-chlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
-
best substrate
-
-
?
3-chlorophenol + NADPH + O2
4-chlorocatechol + NADP+ + H2O
Soil bacterium
-
230% of the activity with 2,4-dichlorophenol
-
-
?
3-chlorophenol + NADPH + O2
?
-
-
-
-
?
4-bromo-2-chlorophenol + NADPH + O2
5-bromo-3-chlorocatechol + NADP+ + H2O
Soil bacterium
-
86% of the activity with 2,4-dichlorophenol
-
-
?
4-bromophenol + NADPH + O2
?
4-chloro-2-methylphenol + NADPH + O2
5-chloro-3-methylcatechol + NADP+ + H2O
Soil bacterium
-
95% of the activity with 2,4-dichlorophenol
-
-
?
4-chloro-2-methylphenol + NADPH + O2
?
4-chloro-2-nitrophenol + NADPH + O2
?
-
-
-
-
?
4-chlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
4-chlorophenol + NADPH + O2
5-chlorocatechol + NADP+ + H2O
4-chlorophenol + NADPH + O2
?
additional information
?
-
2,3-dichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
26% activity compared to 2,4-dichlorophenol as substrate
-
-
?
2,3-dichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
26% activity compared to 2,4-dichlorophenol as substrate
-
-
?
2,3-dichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
-
-
-
-
?
2,3-dichlorophenol + NADPH + O2
?
-
-
-
-
?
2,3-dichlorophenol + NADPH + O2
?
Proteobacteria S1
-
-
-
-
?
2,4,5-trichlorophenol + NADPH + O2
?
-
-
-
-
?
2,4,5-trichlorophenol + NADPH + O2
?
-
-
-
-
?
2,4,5-trichlorophenol + NADPH + O2
?
-
-
-
-
?
2,4,5-trichlorophenol + NADPH + O2
?
Proteobacteria S1
-
-
-
-
?
2,4-dibromophenol + NADPH + O2
?
-
-
-
-
?
2,4-dibromophenol + NADPH + O2
?
-
-
-
-
?
2,4-dibromophenol + NADPH + O2
?
-
-
-
-
?
2,4-dibromophenol + NADPH + O2
?
Proteobacteria S1
-
-
-
-
?
2,4-dichlorophenol + NADH + H+ + O2
3,5-dichlorocatechol + NAD+ + H2O
-
-
-
-
r
2,4-dichlorophenol + NADH + H+ + O2
3,5-dichlorocatechol + NAD+ + H2O
-
-
-
-
r
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
best substrate
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
best substrate
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
Soil bacterium
-
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
Proteobacteria S1
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2-chlorophenol + NADPH + O2
?
-
incompletely metabolized
-
-
?
2-chlorophenol + NADPH + O2
?
-
incompletely metabolized
-
-
?
2-chlorophenol + NADPH + O2
?
-
incompletely metabolized
-
-
?
2-chlorophenol + NADPH + O2
?
-
-
-
-
?
3,4-dichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
30% activity compared to 2,4-dichlorophenol as substrate
-
-
?
3,4-dichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
30% activity compared to 2,4-dichlorophenol as substrate
-
-
?
3,4-dichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
-
low activity
-
-
?
4-bromophenol + NADPH + O2
?
-
incompletely metabolized
-
-
?
4-bromophenol + NADPH + O2
?
-
-
-
-
?
4-chloro-2-methylphenol + NADPH + O2
?
-
-
-
-
?
4-chloro-2-methylphenol + NADPH + O2
?
-
-
-
-
?
4-chloro-2-methylphenol + NADPH + O2
?
-
-
-
-
?
4-chloro-2-methylphenol + NADPH + O2
?
-
-
-
-
?
4-chloro-2-methylphenol + NADPH + O2
?
-
-
-
-
?
4-chloro-2-methylphenol + NADPH + O2
?
Proteobacteria S1
-
-
-
-
?
4-chlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
68% activity compared to 2,4-dichlorophenol as substrate
-
-
?
4-chlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
68% activity compared to 2,4-dichlorophenol as substrate
-
-
?
4-chlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
-
-
-
-
?
4-chlorophenol + NADPH + O2
5-chlorocatechol + NADP+ + H2O
-
-
-
-
?
4-chlorophenol + NADPH + O2
5-chlorocatechol + NADP+ + H2O
-
-
-
-
?
4-chlorophenol + NADPH + O2
?
-
-
-
-
?
4-chlorophenol + NADPH + O2
?
-
-
-
-
?
4-chlorophenol + NADPH + O2
?
-
-
-
-
?
additional information
?
-
-
pseudosubstrates evoke oxidation of NAD(P)H and oxygen consumption without themselves undergoing hydroxylation, the product is hydrogen peroxide: 2,5-dichlorophenol, 2,6-dichlorophenol, 3,4-dichlorophenol, 2, 4,5-trichlorophenol
-
-
?
additional information
?
-
the enzyme shows a narrow substrate specificity with chlorophenols, 3,5-dichlorophenol is a poor substrate, overview
-
-
?
additional information
?
-
-
the enzyme shows a narrow substrate specificity with chlorophenols, 3,5-dichlorophenol is a poor substrate, overview
-
-
?
additional information
?
-
the enzyme shows a narrow substrate specificity with chlorophenols, 3,5-dichlorophenol is a poor substrate, overview
-
-
?
additional information
?
-
-
the enzyme catabolizes 2,4-dichlorophenoxyacetate and 4-chloro-2-methylphenoxyacetate, using tfd functions carried on plasmid pJP4, tfdA cleaves the ether bonds of these herbicides to produce 2,4-dichlorophenol and 4-chloro-2-methylphenol, respectively, these intermediates can be degraded by two chlorophenol hydroxylases encoded by the tfdBI and tfdBII genes to produce the respective chlorocatechols, degradation pathway, a balance between chlorophenol-producing and chlorophenol-consuming reactions is necessary for growth on these compounds, overview
-
-
?
additional information
?
-
-
the enzyme catabolizes 2,4-dichlorophenoxyacetate and 4-chloro-2-methylphenoxyacetate, using tfd functions carried on plasmid pJP4, tfdA cleaves the ether bonds of these herbicides to produce 2,4-dichlorophenol and 4-chloro-2-methylphenol, respectively, these intermediates can be degraded by two chlorophenol hydroxylases encoded by the tfdBI and tfdBII genes to produce the respective chlorocatechols, degradation pathway, a balance between chlorophenol-producing and chlorophenol-consuming reactions is necessary for growth on these compounds, overview
-
-
?
additional information
?
-
-
the 2,4-DCP hydroxylase (TfdB-JLU) exhibits broad substrate specificity for chlorophenols (CPs) and their homologues. Substrate specificity of wild-type and mutant P316Q enzymes, overview
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
4-chloro-2-methylphenol + NADPH + O2
?
4-chlorophenol + NADPH + O2
5-chlorocatechol + NADP+ + H2O
additional information
?
-
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
best substrate
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
best substrate
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
Proteobacteria S1
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
4-chloro-2-methylphenol + NADPH + O2
?
-
-
-
-
?
4-chloro-2-methylphenol + NADPH + O2
?
-
-
-
-
?
4-chlorophenol + NADPH + O2
5-chlorocatechol + NADP+ + H2O
-
-
-
-
?
4-chlorophenol + NADPH + O2
5-chlorocatechol + NADP+ + H2O
-
-
-
-
?
additional information
?
-
-
the enzyme catabolizes 2,4-dichlorophenoxyacetate and 4-chloro-2-methylphenoxyacetate, using tfd functions carried on plasmid pJP4, tfdA cleaves the ether bonds of these herbicides to produce 2,4-dichlorophenol and 4-chloro-2-methylphenol, respectively, these intermediates can be degraded by two chlorophenol hydroxylases encoded by the tfdBI and tfdBII genes to produce the respective chlorocatechols, degradation pathway, a balance between chlorophenol-producing and chlorophenol-consuming reactions is necessary for growth on these compounds, overview
-
-
?
additional information
?
-
-
the enzyme catabolizes 2,4-dichlorophenoxyacetate and 4-chloro-2-methylphenoxyacetate, using tfd functions carried on plasmid pJP4, tfdA cleaves the ether bonds of these herbicides to produce 2,4-dichlorophenol and 4-chloro-2-methylphenol, respectively, these intermediates can be degraded by two chlorophenol hydroxylases encoded by the tfdBI and tfdBII genes to produce the respective chlorocatechols, degradation pathway, a balance between chlorophenol-producing and chlorophenol-consuming reactions is necessary for growth on these compounds, overview
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malfunction
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analysis of substrate binding by the mutant P316Q and the wild-type enzyme, docking study, overview
metabolism
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the enzyme is involved in degradation of the herbicide 2,4-dichlorophenoxyacetic acid
metabolism
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the enzyme is involved in the catabolism of 2,4-dichlorophenol, pathways, overview
metabolism
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two dcm gene clusters essential for the degradation of diclofop-methyl in a microbial consortium of Rhodococcus sp. JT-3 and Brevundimonas sp. JT-9
metabolism
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two dcm gene clusters essential for the degradation of diclofop-methyl in a microbial consortium of Rhodococcus sp. JT-3 and Brevundimonas sp. JT-9
metabolism
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the enzyme is involved in the catabolism of 2,4-dichlorophenol, pathways, overview
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metabolism
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the enzyme is involved in the catabolism of 2,4-dichlorophenol, pathways, overview
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physiological function
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2,4-dichlorophenol hydroxylase (2,4-DCP hydroxylase) is a key enzyme in the degradation of 2,4-dichlorophenoxyacetic acid through the hydroxylation step in many bacteria
physiological function
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degradation of 2,4-dichlorophenol (2,4-DCP) by phenol adapted bacterium Bacillus licheniformis strain SL10 (MTCC 25059) at a relatively fast rate. The organism exhibits tolerance to 150 ppm of 2,4-DCP and shows a linear relationship between the growth and substrate concentration, the inhibitory concentration is 55.74 mg/l, kinetics, overview. The degradation efficiency of the organism is 74% under optimum conditions but increases to 97% when the growth medium contains nil sodium chloride. The organism follows a meta-cleavage pathway while degrading 2,4-DCP
physiological function
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the consortium L1 of Rhodococcus sp. JT-3 and Brevundimonas sp. JT-9 is able to degrade diclofop-methyl (DCM) through a synergistic metabolism, molecular mechanism of DCM degradation, overview. DCM is initially transformed by strain JT-3 to diclofop acid and then by strain JT-9 to 2-(4-hydroxyphenoxy) propionic acid as well as 2,4-dichlorophenol (DCP). DCP is the first intermediate during the degradation of herbicide 2,4-dichlorophenoxyacetic acid (2,4-D), wherein 2,4-D is converted into 3,5-dichlorocatechol by the 2,4-dichlorophenol hydroxylase
physiological function
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the consortium L1 of Rhodococcus sp. JT-3 and Brevundimonas sp. JT-9 is able to degrade diclofop-methyl (DCM) through a synergistic metabolism, molecular mechanism of DCM degradation, overview. DCM is initially transformed by strain JT-3 to diclofop acid and then by strain JT-9 to 2-(4-hydroxyphenoxy) propionic acid as well as 2,4-dichlorophenol (DCP). DCP is the first intermediate during the degradation of herbicide 2,4-dichlorophenoxyacetic acid (2,4-D), wherein 2,4-D is converted into 3,5-dichlorocatechol by the 2,4-dichlorophenol hydroxylase
physiological function
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degradation of 2,4-dichlorophenol (2,4-DCP) by phenol adapted bacterium Bacillus licheniformis strain SL10 (MTCC 25059) at a relatively fast rate. The organism exhibits tolerance to 150 ppm of 2,4-DCP and shows a linear relationship between the growth and substrate concentration, the inhibitory concentration is 55.74 mg/l, kinetics, overview. The degradation efficiency of the organism is 74% under optimum conditions but increases to 97% when the growth medium contains nil sodium chloride. The organism follows a meta-cleavage pathway while degrading 2,4-DCP
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physiological function
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degradation of 2,4-dichlorophenol (2,4-DCP) by phenol adapted bacterium Bacillus licheniformis strain SL10 (MTCC 25059) at a relatively fast rate. The organism exhibits tolerance to 150 ppm of 2,4-DCP and shows a linear relationship between the growth and substrate concentration, the inhibitory concentration is 55.74 mg/l, kinetics, overview. The degradation efficiency of the organism is 74% under optimum conditions but increases to 97% when the growth medium contains nil sodium chloride. The organism follows a meta-cleavage pathway while degrading 2,4-DCP
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additional information
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molecular docking experiments of the enzyme TfdB-JLU's homology model with its natural substrate 2,4-dichlorophenol (with template PDB ID 5brt) reveals that the phenyl rings of 2,4-DCP form strong interactions with residues His47, Ile48, Trp222, Pro316, and Phe424. These residues are found to be important for substrate binding in the active site
additional information
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synthesis of hybrid nanoflowers (hNFs) formed from cold-adapted 2,4-dichlorophenol hydroxylase (tfdBJLU) and Cu3(PO4)2 x 3H2O. Analysis of the influence of experimental factors, such as the pH of the solution mixture and the enzyme and Cu2+ concentrations, on the activity of the prepared tfdB-JLU-hNFs. 200 mM is the optimal Cu2+ concentration to get tfdB-JLU-hNFs with highest activity recovery, method optimization, overview. The tfdB-JLU-hNFs exhibit excellent durability with 58.34% residual activity after six successive cycles, and up to 90.58% residual activity after 20 days of storage. This multistage and hierarchical flower-like structure can effectively increase enzyme activity and stability with respect to those of the free enzyme. The satisfactory removal rate of 2,4-dichlorophenol catalyzed by tfdB-JLU-hNFs suggests that this immobilized enzyme exhibits great potential for application in bioremediation
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Beadle, C.A.; Smith, A.R.W.
The purification and properties of 2,4-dichlorophenol hydroxylase from a strain of Acinetobacter species
Eur. J. Biochem.
123
323-332
1982
Acinetobacter sp.
brenda
Liu, T.; Chapman, P.J.
Purification and properties of a plasmid-encoded 2,4-dichlorophenol hydroxylase
FEBS Lett.
173
314-318
1984
Cupriavidus necator, Cupriavidus necator JMP 134-1
brenda
Radjendirane, V.; Bhat, M.A.; Vaidyanathan, C.S.
Affinity purification and characterization of 2,4-dichlorophenol hydroxylase from Pseudomonas cepacia [published erratum appears in Arch Biochem Biophys 1992 Jul;296(1):354]
Arch. Biochem. Biophys.
288
169-176
1991
Burkholderia cepacia
brenda
Farhana, L.; Fulthorpe, R.R.; Harbour, C.; New, P.B.
Monoclonal antibodies to 2,4-dichlorophenol hydroxylase as probes for the 2,4-D-degradative phenotype
Can. J. Microbiol.
44
920-928
1998
Cupriavidus necator, Variovorax paradoxus, Burkholderia cepacia, Burkholderia mallei, Burkholderia sp., Sphingomonas paucimobilis, Pseudomonas putida, Rhodoferax fermentans, Rhodopseudomonas palustris, Sphingomonas sp., Sphingomonas sp. B6-5, Rhodopseudomonas palustris M1, Burkholderia mallei TFD6, Burkholderia sp. RASC, Sphingomonas sp. EML146, Variovorax paradoxus 2811P, Rhodoferax fermentans B6-9, Pseudomonas putida PaW85, Burkholderia sp. TFD2, Sphingomonas sp. TFD44, Cupriavidus necator JMP 134-1, Rhodoferax fermentans TFD23, Burkholderia cepacia DBO131, Cupriavidus necator TFD41, Sphingomonas paucimobilis 1443, Sphingomonas sp. B6-10
brenda
Farhana, L.; New, P.B.
The 2,4-dichlorophenol hydroxylase of Alcaligenes eutrophus JMP134 is a homotetramer
Can. J. Microbiol.
43
202-205
1997
Cupriavidus necator, Cupriavidus necator JMP 134-1
brenda
Makdessi, K.; Lechner, U.
Purification and characterization of 2,4-dichlorophenol hydroxylase isolated from a bacterium of the alpha-2 subgroup of the Proteobacteria
FEMS Microbiol. Lett.
157
95-101
1997
Proteobacteria, Proteobacteria S1
brenda
Ledger, T.; Pieper, D.H.; Gonzalez, B.
Chlorophenol hydroxylases encoded by plasmid pJP4 differentially contribute to chlorophenoxyacetic acid degradation
Appl. Environ. Microbiol.
72
2783-2792
2006
Cupriavidus necator, Paraburkholderia xenovorans
brenda
Huong, N.L.; Itoh, K.; Miyamoto, M.; Suyama, K.; Yamamoto, H.
Chlorophenol hydroxylase activity encoded by TfdB from 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading Bradyrhizobium sp. strain RD5-C2
Biosci. Biotechnol. Biochem.
71
1691-1696
2007
Bradyrhizobium sp. (A5LGH2), Bradyrhizobium sp., Bradyrhizobium sp. RD5-C2 (A5LGH2)
brenda
Lu, Y.; Yu, Y.; Zhou, R.; Sun, W.; Dai, C.; Wan, P.; Zhang, L.; Hao, D.; Ren, H.
Cloning and characterisation of a novel 2,4-dichlorophenol hydroxylase from a metagenomic library derived from polychlorinated biphenyl-contaminated soil
Biotechnol. Lett.
33
1159-1167
2011
Soil bacterium
brenda
Xia, Z.Y.; Zhang, L.; Zhao, Y.; Yan, X.; Li, S.P.; Gu, T.; Jiang, J.D.
Biodegradation of the Herbicide 2,4-Dichlorophenoxyacetic Acid by a New Isolated Strain of Achromobacter sp. LZ35
Curr. Microbiol.
74
193-202
2017
Achromobacter sp. LZ35
brenda
Chris Felshia, S.; AshwinKarthick, N.; Thilagam, R.; Gnanamani, A.
Elucidation of 2,4-dichlorophenol degradation by Bacillus licheniformis strain SL10
Environ. Technol.
41
366-377
2020
Bacillus licheniformis, Bacillus licheniformis MTCC 25059, Bacillus licheniformis SL10
brenda
Zhang, H.; Yu, T.; Li, J.; Wang, Y.R.; Wang, G.L.; Li, F.; Liu, Y.; Xiong, M.H.; Ma, Y.Q.
Two dcm gene clusters essential for the degradation of diclofop-methyl in a microbial consortium of Rhodococcus sp. JT-3 and Brevundimonas sp. JT-9
J. Agric. Food Chem.
66
12217-12226
2018
Rhodococcus sp. JT-3, Brevundimonas sp. JT-9
brenda
Fang, X.; Zhang, C.; Qian, X.; Yu, D.
Self-assembled 2,4-dichlorophenol hydroxylase-inorganic hybrid nanoflowers with enhanced activity and stability
RSC Adv.
8
20976-20981
2018
uncultured bacterium
brenda
Wang, Y.; Zhang, C.; An, S.; Fang, X.; Yu, D.
Engineering substrate promiscuity in 2,4-dichlorophenol hydroxylase by in silico design
RSC Adv.
8
21184-21190
2018
uncultured bacterium
brenda