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(6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2
scopolamine + succinate + CO2
(6S)-hydroxyhyoscyamine + succinate + CO2
L-hyoscyamine + 2-oxoglutarate + O2
-
-
-
r
2-hydroxy-3-phenylpropionyltropine + 2-oxoglutarate + O2
2-hydroxy-3-phenylpropionyl-6-hydroxytropine + succinate + CO2
-
15% of the activity with L-hyoscyamine
-
?
2-oxoglutarate + p-hydroxyatropine + O2
?
-
26% of the activity with L-hyoscyamine
-
-
?
2-oxoglutarate + trans-cinnamoyltropine + O2
succinate + trans-cinnamoyl-6-hydroxytropine + CO2
-
39% of the activity with L-hyoscyamine
-
?
3-hydroxy-3-phenylpropionyltropine + 2-oxoglutarate + O2
3-hydroxy-3-phenylpropionyl-6-hydroxytropine + succinate + CO2
-
56% of the activity with L-hyoscyamine
-
?
6,7-dehydrohyoscyamine + 2-oxoglutarate + O2
scopolamine + succinate + CO2
-
119% of the activity with L-hyoscyamine
-
?
6beta-hydroxyhyoscyamine
scopolamine
-
weak epoxidase activity, 2-5% of the hydroxylase activity
-
-
?
6beta-hydroxyhyoscyamine + ?
scopolamine + ?
-
-
-
?
8-methyl-8-azabicyclo[3.2.1]octan-3-yl 2-(4-(dimethylamino)phenyl)acetate + 2-oxoglutarate + O2
?
-
-
-
-
?
8-methyl-8-azabicyclo[3.2.1]octan-3-yl benzoate + 2-oxoglutarate + O2
?
-
-
-
-
?
apoatropine + 2-oxoglutarate + O2
6-hydroxyapoatropine + succinate + CO2
-
45% of the activity with L-hyoscyamine
-
?
hyoscyamine + O2 + 2-oxoglutarate + O2
scopolamine + succinate + CO2
-
-
-
-
?
isobutyltropine + 2-oxoglutarate + O2
?
-
15% of the activity with L-hyoscyamine
-
-
?
L-homatropine + 2-oxoglutarate + O2
6-hydroxyhomatropine + succinate + CO2
-
81% of the activity with L-hyoscyamine
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO2
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
L-norhyoscyamine + 2-oxoglutarate + O2
6-hydroxynorhyoscyamine + succinate + CO2
-
81% of the activity with L-hyoscyamine
-
?
noratropine-N-acetic acid + 2-oxoglutarate + O2
?
-
17% of the activity with L-hyoscyamine
-
-
?
phenylacetyltropine + 2-oxoglutarate + O2
6-hydroxyphenylacetyltropine + succinate + CO2
-
81% of the activity with L-hyoscyamine
-
?
phenylalanyltropine + 2-oxoglutarate + O2
?
-
8% of the activity with L-hyoscyamine
-
-
?
additional information
?
-
(6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2
scopolamine + succinate + CO2
-
-
-
?
(6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2
scopolamine + succinate + CO2
key enzyme in the biosynthesis pathway of scopolamine
-
-
?
(6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2
scopolamine + succinate + CO2
-
-
-
?
(6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2
scopolamine + succinate + CO2
-
-
-
-
?
(6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2
scopolamine + succinate + CO2
-
-
-
-
ir
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO
-
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO
the bifunctional enzyme also catalyzes the conversion of (6S)-6beta-hydroxyhyoscyamine to scopolamine, cf. EC 1.14.20.13
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO2
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO2
-
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO2
the enzyme catalyzes the hydroxylation of (-)-hyoscyamine and the subsequent epoxidation of 6beta-hydroxyhyoscyamine to form scopolamine, a natural alkaloid
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO2
-
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO2
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO2
MH392211
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO2
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO2
-
-
H6H produces 6beta-hydroxyhyoscyamine and its derivative, scopolamine, the DmrH6H epoxidase activity is low compared to the hydroxylase activity
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO2
-
the binding of the substrates, hyoscyamine and 2-oxoglutarate, to the enzyme induces significant conformational changes
H6H produces 6beta-hydroxyhyoscyamine and its derivative, scopolamine, the DmrH6H epoxidase activity is low compared to the hydroxylase activity
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO2
Glu116 interacts with the tertiary amine located on the hyoscyamine tropane moiety and Tyr326 forms CH-pi hydrogen bonds with the hyoscyamine phenyl ring
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO2
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
key enzyme in the biosynthesis pathway of scopolamine
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
Hyoscyamus gyorffi
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
-
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
-
-
-
-
ir
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
-
only the L-isomer of hyoscyamine serves as substrate, D-hyoscyamine is nearly inactive
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
-
the enzyme catalyzes two consecutive oxidation reactions leading from hyoscyamine to scopolamine
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
-
enzyme is involved in biosynthetic pathway leading to scopolamine
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
-
-
-
?
additional information
?
-
the enzyme also catalyzes the reaction of EC 1.14.11.14
-
-
?
additional information
?
-
-
the enzyme also catalyzes the reaction of EC 1.14.11.14
-
-
?
additional information
?
-
-
the enzyme also catalyzes the reaction of EC 1.14.11.14
-
-
?
additional information
?
-
-
hyoscyamine 6beta-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, converts L-hyoscyamine to its 6,7-epoxy derivative, scopolamine, in two sequential steps. 6,7-Dehydrohyoscyamine, a potential precursor for the last step of epoxidation, is an obligatory intermediate in the biosynthesis of scopolamine
-
-
?
additional information
?
-
-
hyoscyamine 6beta-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, converts L-hyoscyamine to its 6,7-epoxy derivative, scopolamine, in two sequential steps. The catalytic efficiency of AbH6H, especially for the second oxidation, which is the reaction of EC 1.14.11.14, is low. The epoxidation step is much slower than the hydroxylation step. Substrate analogues 1-methylpiperidin-4-yl 2-phenylacetate, 8-methyl-8-azabicyclo[3.2.1]octan-3-yl 3-phenylpropanoate, 8-oxabicyclo[3.2.1]octan-3-yl 2-phenylacetate, and 4 are no substrates for the enzyme
-
-
?
additional information
?
-
the enzyme also catalyzes the reaction of EC 1.14.11.14
-
-
?
additional information
?
-
-
high specificity for 2-oxoglutarate in hydroxylation
-
-
?
additional information
?
-
the enzyme also catalyzes the reaction of EC 1.14.11.14
-
-
?
additional information
?
-
-
the enzyme also catalyzes the reaction of EC 1.14.11.14
-
-
?
additional information
?
-
-
methyl jasmonate and salicylic acid increase the expression of the enzyme
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2
scopolamine + succinate + CO2
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO2
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
additional information
?
-
(6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2
scopolamine + succinate + CO2
key enzyme in the biosynthesis pathway of scopolamine
-
-
?
(6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2
scopolamine + succinate + CO2
-
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO
-
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO2
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO2
-
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO2
the enzyme catalyzes the hydroxylation of (-)-hyoscyamine and the subsequent epoxidation of 6beta-hydroxyhyoscyamine to form scopolamine, a natural alkaloid
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO2
-
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO2
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO2
MH392211
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO2
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO2
-
-
H6H produces 6beta-hydroxyhyoscyamine and its derivative, scopolamine, the DmrH6H epoxidase activity is low compared to the hydroxylase activity
-
?
L-hyoscyamine + 2-oxoglutarate + O2
(6S)-hydroxyhyoscyamine + succinate + CO2
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
key enzyme in the biosynthesis pathway of scopolamine
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
-
-
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
-
the enzyme catalyzes two consecutive oxidation reactions leading from hyoscyamine to scopolamine
-
-
?
L-hyoscyamine + 2-oxoglutarate + O2
6beta-hydroxyhyoscyamine + succinate + CO2
-
enzyme is involved in biosynthetic pathway leading to scopolamine
-
?
additional information
?
-
-
hyoscyamine 6beta-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, converts L-hyoscyamine to its 6,7-epoxy derivative, scopolamine, in two sequential steps. 6,7-Dehydrohyoscyamine, a potential precursor for the last step of epoxidation, is an obligatory intermediate in the biosynthesis of scopolamine
-
-
?
additional information
?
-
-
methyl jasmonate and salicylic acid increase the expression of the enzyme
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Hashimoto, T.; Yamada, Y.
Purification and characterization of hyoscyamine 6 beta-hydroxylase from root cultures of Hyoscyamus niger L. Hydroxylase and epoxidase activities in the enzyme preparation
Eur. J. Biochem.
164
277-285
1987
Hyoscyamus niger
brenda
Matsuda, J.; Okabe, S.; Hashimoto, T.; Yamada, Y.
Molecular cloning of hyoscyamine 6 beta-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, from cultured roots of Hyoscyamus niger
J. Biol. Chem.
266
9460-9464
1991
Hyoscyamus niger
brenda
Hashimoto, T.; Yamada, Y.
Hyoscyamine 6beta-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, an alkaloid-producing root cultures
Plant Physiol.
81
619-625
1986
Atropa belladonna, Datura metel, Duboisia leichhardtii, Hyoscyamus albus, Hyoscyamus gyorffi, Hyoscyamus muticus, Hyoscyamus niger, Hyoscyamus pusillus
brenda
Rocha, P.; Stenzel, O.; Parr, A.; Walton, N.; Christou, P.; Drager, B.; Leech, M.J.
Functional expression of tropinone reductase I (trI) and hyoscyamine-6beta-hydroxylase (h6h) from Hyoscyamus niger in Nicotiana tabacum
Plant Sci.
162
905-913
2002
Hyoscyamus niger
-
brenda
Yun, D.J.; Hashimoto, T.; Yamada, Y.
Transgenic tobacco plants with two consecutive oxidation reactions catalyzed by hyoscyamine 6b-hydroxylase
Biosci. Biotechnol. Biochem.
57
502-503
1993
Hyoscyamus niger
-
brenda
Suzuki, K.I.; Yun, D.J.; Chen, X.Y.; Yamada, Y.; Hashimoto, T.
An Atropa belladonna hyoscyamine 6beta-hydroxylase gene is differentially expressed in the root pericycle and anthers
Plant Mol. Biol.
40
141-152
1999
Atropa belladonna
brenda
Matsuda, J.; Hashimoto, T.; Yamada, Y.
Analysis of active-site residues in hyoscyamine 6beta-hydroxylase
Plant Biotechnol.
14
51-57
1997
Hyoscyamus niger
-
brenda
Palazon, J.; Moyano, E.; Cusido, R.M.; Bonfill, M.; Oksman-Caldentey, K.M.; Pinol, M.T.
Alkaloid production in Duboisia hybrid hairy roots and plants overexpressing the h6h gene
Plant Sci.
165
1289-1295
2003
Hyoscyamus niger
-
brenda
Kang, S.M.; Jung, H.Y.; Kang, Y.M.; Yun, D.J.; Bahk, J.D.; Yang, J.k.; Choi, M.S.
Effects of methyl jasmonate and salicylic acid on the production of tropane alkaloids and the expression of PMT and H6H in adventitious root cultures of Scopolia parviflora
Plant Sci.
166
745-751
2004
Scopolia parviflora
brenda
Liu, T.; Zhu, P.; Cheng, K.D.; Meng, C.; He, H.X.
Molecular cloning, expression and characterization of hyoscyamine 6beta-hydroxylase from hairy roots of Anisodus tanguticus
Planta Med.
71
249-253
2005
Anisodus tanguticus (Q6V0J1), Anisodus tanguticus
brenda
Zarate, R.; el Jaber-Vazdekis, N.; Medina, B.; Ravelo, A.G.
Tailoring tropane alkaloid accumulation in transgenic hairy roots of Atropa baetica by over-expressing the gene encoding hyoscyamine 6beta-hydroxylase
Biotechnol. Lett.
28
1271-1277
2006
Hyoscyamus niger
brenda
Rahman, L.u.; Kitamura, Y.; Yamaguchi, J.; Mukai, M.; Akiyama, K.; Yamamoto, H.; Muranaka, T.; Ikenaga, T.
Exogenous plant H6H but not bacterial HCHL gene is expressed in Duboisia leichhardtii hairy roots and affects tropane alkaloid production
Enzyme Microb. Technol.
39
1183-1189
2006
Hyoscyamus niger
-
brenda
Haekkinen, S.T.; Moyano, E.; Cusido, R.M.; Palazon, J.; Pinol, M.T.; Oksman-Caldentey, K.M.
Enhanced secretion of tropane alkaloids in Nicotiana tabacum hairy roots expressing heterologous hyoscyamine-6beta-hydroxylase
J. Exp. Bot.
56
2611-2618
2005
Hyoscyamus niger
brenda
Kai, G.; Chen, J.; Li, L.; Zhou, G.; Zhou, L.; Zhang, L.; Chen, Y.; Zhao, L.
Molecular cloning and characterization of a new cDNA encoding hyoscyamine 6beta-hydroxylase from roots of Anisodus acutangulus
J. Biochem. Mol. Biol.
40
715-722
2007
Anisodus acutangulus (A2IBI0), Anisodus acutangulus
brenda
Cardillo, A.B.; Talou, J.R.; Giulietti, A.M.
Expression of Brugmansia candida hyoscyamine 6beta-hydroxylase gene in Saccharomyces cerevisiae and its potential use as biocatalyst
Microb. Cell Fact.
7
17
2008
Brugmansia x candida (B2CNC8)
brenda
Higa, A.; Miyamoto, E.; Rahman, L.u.; Kitamura, Y.
Root tip-dependent, active riboflavin secretion by Hyoscyamus albus hairy roots under iron deficiency
Plant Physiol. Biochem.
46
452-460
2008
Hyoscyamus albus
brenda
Jaber-Vazdekis, N.; Gonzalez, C.; Ravelo, A.G.; Zarate, R.
Cloning, characterization and analysis of expression profiles of a cDNA encoding a hyoscyamine 6beta-hydroxylase (H6H) from Atropa baetica Willk
Plant Physiol. Biochem.
47
20-25
2009
Atropa baetica (A9Q1G4), Atropa baetica
brenda
Pramod, K.K.; Singh, S.; Jayabaskaran, C.
Biochemical and structural characterization of recombinant hyoscyamine 6beta-hydroxylase from Datura metel L
Plant Physiol. Biochem.
48
966-970
2010
Datura metel
brenda
Li, J.; van Belkum, M.J.; Vederas, J.C.
Functional characterization of recombinant hyoscyamine 6beta-hydroxylase from Atropa belladonna
Bioorg. Med. Chem.
20
4356-4363
2012
Atropa belladonna
brenda
Kai, G.; Liu, Y.; Wang, X.; Yang, S.; Fu, X.; Luo, X.; Liao, P.
Functional identification of hyoscyamine 6beta-hydroxylase from Anisodus acutangulus and overproduction of scopolamine in genetically-engineered Escherichia coli
Biotechnol. Lett.
33
1361-1365
2011
Anisodus acutangulus
brenda
Kai, G.; Zhang, A.; Guo, Y.; Li, L.; Cui, L.; Luo, X.; Liu, C.; Xiao, J.
Enhancing the production of tropane alkaloids in transgenic Anisodus acutangulus hairy root cultures by over-expressing tropinone reductase I and hyoscyamine-6beta-hydroxylase
Mol. Biosyst.
8
2883-2890
2012
Anisodus acutangulus (A2IBI0), Anisodus acutangulus
brenda
Vakili, B.; Karimi, F.; Sharifi, M.; Behmanesh, M.
Chromium-induced tropane alkaloid production and H6H gene expression in Atropa belladonna L. (Solanaceae) in vitro-propagated plantlets
Plant Physiol. Biochem.
52
98-103
2012
Atropa belladonna (Q9XJ43)
brenda
Dehghan, E.; Shahriari Ahmadi, F.; Ghotbi Ravandi, E.; Reed, D.W.; Covello, P.S.; Bahrami, A.R.
An atypical pattern of accumulation of scopolamine and other tropane alkaloids and expression of alkaloid pathway genes in Hyoscyamus senecionis
Plant Physiol. Biochem.
70
188-194
2013
Hyoscyamus senecionis (J7HDC2), Hyoscyamus senecionis
brenda
Cardillo, A.; Perassolo, M.; Sartuqui, M.; Rodriguez Talou, J.; Giulietti, A.
Production of tropane alkaloids by biotransformation using recombinant Escherichia coli whole cells
Biochem. Eng. J.
125
180-189
2017
Brugmansia x candida (B2CNC8)
-
brenda
Cao, Y.D.; He, Y.C.; Li, H.; Kai, G.Y.; Xu, J.H.; Yu, H.L.
Efficient biosynthesis of rare natural product scopolamine using E. coli cells expressing a S14P/K97A mutant of hyoscyamine 6beta-hydroxylase AaH6H
J. Biotechnol.
211
123-129
2015
Anisodus acutangulus (A2IBI0), Anisodus acutangulus
brenda
Xia, K.; Liu, X.; Zhang, Q.; Qiang, W.; Guo, J.; Lan, X.; Chen, M.; Liao, Z.
Promoting scopolamine biosynthesis in transgenic Atropa belladonna plants with pmt and h6h overexpression under field conditions
Plant Physiol. Biochem.
106
46-53
2016
Hyoscyamus niger (P24397), Hyoscyamus niger
brenda
Lan, X.; Zeng, J.; Liu, K.; Zhang, F.; Bai, G.; Chen, M.; Liao, Z.; Huang, L.
Comparison of two hyoscyamine 6beta-hydroxylases in engineering scopolamine biosynthesis in root cultures of Scopolia lurida
Biochem. Biophys. Res. Commun.
497
25-31
2018
Hyoscyamus niger (P24397), Hyoscyamus niger
brenda
Naik, T.; Vanitha, S.; Rajvanshi, P.; Chandrika, M.; Kamalraj, S.; Jayabaskaran, C.
Novel microbial sources of tropane alkaloids first report of production by endophytic fungi isolated from Datura metel L
Curr. Microbiol.
75
206-212
2018
Datura metel (Q6EZB3)
brenda
Kluza, A.; Wojdyla, Z.; Mrugala, B.; Kurpiewska, K.; Porebski, P.J.; Niedzialkowska, E.; Minor, W.; Weiss, M.S.; Borowski, T.
Regioselectivity of hyoscyamine 6beta-hydroxylase-catalysed hydroxylation as revealed by high-resolution structural information and QM/MM calculations
Dalton Trans.
49
4454-4469
2020
Datura metel (Q6EZB3)
brenda
Fischer, C.; Kwon, M.; Ro, D.K.; van Belkum, M.J.; Vederas, J.C.
Isolation, expression and biochemical characterization of recombinant hyoscyamine-6beta-hydroxylase from Brugmansia sanguinea - tuning the scopolamine production
MedChemComm
9
888-892
2018
Brugmansia sanguinea
brenda
Kohnen, K.; Sezgin, S.; Spiteller, M.; Hagels, H.; Kayser, O.
Localization and organization of scopolamine biosynthesis in Duboisia myoporoides R. Br
Plant Cell Physiol.
59
107-118
2018
Duboisia myoporoides (A0A1S6LXV6)
brenda
Li, Q.; Zhu, T.; Zhang, R.; Bu, Q.; Yin, J.; Zhang, L.; Chen, W.
Molecular cloning and functional analysis of hyoscyamine 6beta-hydroxylase (H6H) in the poisonous and medicinal plant Datura innoxia mill
Plant Physiol. Biochem.
153
11-19
2020
Datura inoxia (MH392211), Datura inoxia
brenda
Schlesinger, D.; Davidovich Rikanati, R.; Volis, S.; Faigenboim, A.; Vendramin, V.; Cattonaro, F.; Hooper, M.; Oren, E.; Taylor, M.; Sitrit, Y.; Inbar, M.; Lewinsohn, E.
Alkaloid chemodiversity in Mandragora spp. is associated with loss-of-functionality of MoH6H, a hyoscyamine 6beta-hydroxylase gene
Plant Sci.
283
301-310
2019
Mandragora autumnalis (A0A6M6R148)
brenda