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Information on EC 1.1.99.24 - hydroxyacid-oxoacid transhydrogenase and Organism(s) Rattus norvegicus

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IUBMB Comments
4-Hydroxybutanoate and (R)-2-hydroxyglutarate can also act as donors; 4-oxobutanoate can also act as acceptor.
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This record set is specific for:
Rattus norvegicus
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Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
adhfe1, hydroxyacid-oxoacid transhydrogenase, alcohol dehydrogenase iron containing 1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
transhydrogenase, hydroxy acid-oxo acid
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
transhydrogenation
-
-
SYSTEMATIC NAME
IUBMB Comments
(S)-3-hydroxybutanoate:2-oxoglutarate oxidoreductase
4-Hydroxybutanoate and (R)-2-hydroxyglutarate can also act as donors; 4-oxobutanoate can also act as acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
117698-31-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-hydroxybutyrate + 2-oxoglutarate
D-2-hydroxyglutarate + succinate semialdehyde
show the reaction diagram
-
-
-
-
?
4-hydroxybutyrate + 2-oxoglutarate
succinic semialdehyde + 2-hydroxyglutarate
show the reaction diagram
4-hydroxybutyrate + 2-oxoglutarate
succinic semialdehyde + D-2-hydroxyglutarate
show the reaction diagram
D-2-hydroxybutyrate + 2-oxoglutarate
?
show the reaction diagram
-
-
-
-
r
D-2-hydroxyglutarate + succinic semialdehyde
2-oxoglutarate + 4-hydroxybutyrate
show the reaction diagram
L-3-hydroxybutyrate + 2-oxoglutarate
acetoacetate + 2-oxoglutarate
show the reaction diagram
L-3-hydroxybutyrate + succinic semialdehyde
acetoacetate + 4-hydroxybutyrate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-hydroxybutyrate + 2-oxoglutarate
D-2-hydroxyglutarate + succinate semialdehyde
show the reaction diagram
-
-
-
-
?
4-hydroxybutyrate + 2-oxoglutarate
succinic semialdehyde + D-2-hydroxyglutarate
show the reaction diagram
-
-
-
-
r
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
IC50: 0.75 mM
cyanide
-
IC50: 0.075 mM
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no effect by EDTA and EGTA
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.018 - 0.085
2-oxoglutarate
0.06 - 0.3
4-hydroxybutyrate
4.5
D-2-hydroxybutyrate
-
no activity with L-isomer, 30°C, pH 7.1
0.42 - 4.5
D-2-hydroxyglutarate
3
L-3-hydroxybutyrate
-
-
0.0046 - 0.01
Succinic semialdehyde
additional information
additional information
-
no activity with L-isomer of 2-hydroxybutyrate, Km for succinate semialdehyde is below 0.01 mM, 30°C, pH 7.1
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.75
1,10-phenanthroline
-
30°C, pH 7.1
0.075
cyanide
-
30°C, pH 7.1
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.75
1,10-phenanthroline
Rattus norvegicus
-
IC50: 0.75 mM
0.075
cyanide
Rattus norvegicus
-
IC50: 0.075 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
very low enzyme activity
Manually annotated by BRENDA team
-
low enzyme activity
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HOT_RAT
467
0
50226
Swiss-Prot
Mitochondrion (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45000 - 50000
-
-
45000 - 55000
-
gel filtration
50000
-
1 * 50000, about, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 50000, about, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
NAD+ stabilizes during ammonium sulfate precipitation
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1300fold, partial from liver
-
native enzyme from liver 1300fold by precipitation using PEG 6000, anion exchange and hydrophobic interaction chromatography, and gel filtration
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kaufman, E.E.; Nelson, T.
An overview of gamma-hydroxybutyrate catabolism: the role of the cytosolic NADP+-dependent oxidoreductase EC 1.1.1.19 and of a mitochondrial hydroxyacid-oxoacid transhydrogenase in the initial, rate-limiting step in this pathway
Neurochem. Res.
16
965-974
1991
Rattus norvegicus
Manually annotated by BRENDA team
Kaufman, E.E.; Nelson, T.; Fales, H.M.; Levin, D.M.
Isolation and characterization of a hydroxyacid-oxoacid transhydrogenase from rat kidney mitochondria
J. Biol. Chem.
263
16872-16879
1988
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Manually annotated by BRENDA team
Kardon, T.; Noel, G.; Vertommen, D.; Van Schaftingen, E.
Identification of the gene encoding hydroxyacid-oxoacid transhydrogenase, an enzyme that metabolizes 4-hydroxybutyrate
FEBS Lett.
580
2347-2350
2006
Homo sapiens, Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team