Information on EC 1.1.5.3 - glycerol-3-phosphate dehydrogenase

New: Word Map on EC 1.1.5.3
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

SplaateEC_Number,Commentary
EC NUMBER
COMMENTARY
1.1.5.3
-
SplaateRecommended_Name,GO_Number
RECOMMENDED NAME
GeneOntology No.
glycerol-3-phosphate dehydrogenase
-
SplaateReaction,Reaction_id,Commentary,IF(Commentary != '',Organism,'') ,IF(Commentary != '',Literature,'')
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol
show the reaction diagram
ping-pong mechanism
-
sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol
show the reaction diagram
-
-
-
-
SplaateReaction_Type,Organism,Commentary,Literature
SplaatePathway,BRENDA_Link,KEGG_Link,MetaCyc_Link,Source_Database
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
glycerol degradation I
-
-
glycerol-3-phosphate shuttle
-
-
glycerol-3-phosphate to cytochrome bo oxidase electron transfer
-
-
glycerol-3-phosphate to fumarate electron transfer
-
-
glycerophosphodiester degradation
-
-
nitrate reduction IX (dissimilatory)
-
-
nitrate reduction X (periplasmic, dissimilatory)
-
-
degradation of sugar alcohols
-
-
Glycerophospholipid metabolism
-
-
Biosynthesis of secondary metabolites
-
-
SplaateSystematic_Name,Commentary_IUBMB
SYSTEMATIC NAME
IUBMB Comments
sn-glycerol 3-phosphate:quinone oxidoreductase
This flavin-dependent dehydrogenase is an essential membrane enzyme, functioning at the central junction of glycolysis, respiration and phospholipid biosynthesis. In bacteria, the enzyme is localized to the cytoplasmic membrane [6], while in eukaryotes it is tightly bound to the outer surface of the inner mitochondrial membrane [2]. In eukaryotes, this enzyme, together with the cytosolic enzyme EC 1.1.1.8, glycerol-3-phosphate dehydrogenase (NAD+), forms the glycerol-3-phosphate shuttle by which NADH produced in the cytosol, primarily from glycolysis, can be reoxidized to NAD+ by the mitochondrial electron-transport chain [3]. This shuttle plays a critical role in transferring reducing equivalents from cytosolic NADH into the mitochondrial matrix [7,8]. Insect flight muscle uses only CoQ10 as the physiological quinone whereas hamster and rat mitochondria use mainly CoQ9 [4]. The enzyme is activated by calcium [3].
SplaateSynonyms,Organism,Commentary,Literature
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
FAD-dependent glycerol-3-phosphate dehydrogenase
-
-
FAD-dependent glycerol-3-phosphate dehydrogenase
-
FAD-glycerol phosphate dehydrogenase
-
flavoprotein-dependent glycerol-3-phosphate dehydrogenase
-
-
flavoprotein-dependent glycerol-3-phosphate dehydrogenase
Rattus norvegicus Wistar
-
-
-
GlpO
Q3Y0N9
-
glycerol-3-phosphate dehydrogenase
-
-
glycerol-3-phosphate dehydrogenase
-
-
glycerol-3-phosphate dehydrogenase
-
-
glycerol-3-phosphate dehydrogenase 2
-
glycerol-3-phosphate oxidoreductase
-
-
glycerol-3-phosphate oxidoreductase
Rattus norvegicus Wistar
-
-
-
glycerophosphate dehydrogenase
mitochondrial, enzyme-bound FAD
GPDH
-
-
GPDH
Rattus norvegicus Wistar
-
-
-
SplaateCAS_Registry_Number,Commentary
CAS REGISTRY NUMBER
COMMENTARY
9001-49-4
-
SplaateOrganism, Commentary,Literature, Sequence_Code,Sequence_db,Textmining
SplaateGeneral_Information, Organism, Commentary, Literature
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
in the absence of Gpd2, hyperactivation and acrosome reaction are significantly altered, and a few changes in protein tyrosine phosphorylation are observed during sperm capacitation. The testicular size of Gpd2-deficient animals is about 10% smaller than in wild type animals. Gpd2-null animals show a 4% reduction in tubular diameter and about 10% reduction in numbers of both spermatocytes and spermatids per seminiferous tubule
physiological function
-
GPD2 enables tyrosine phosphorylation during sperm capacitation. GPD2 activity is required for reactive oxygen species generation in mouse spermatozoa during capacitation
SplaateSubstrates,Products,id,Organism_Substrates,Commentary_Substrates, Literature_Substrates, Commentary_Products, Literature_Products,Reversibility
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
sn-glycerol 3-phosphate + 1-methyl-2-decyl-3,4-dimethoxybenzoquinone
glycerone phosphate + ?
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + 2,6-dichlorophenolindophenol
glycerone phosphate + reduced 2,6-dichlorophenylindophenol
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + 2,6-dichlorophenolindophenol
glycerone phosphate + reduced 2,6-dichlorophenylindophenol
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + 2,6-dichlorophenolindophenol
glycerone phosphate + reduced 2,6-dichlorophenylindophenol
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + 2,6-dichlorophenolindophenol
glycerone phosphate + reduced 2,6-dichlorophenylindophenol
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + 2,6-dichlorophenolindophenol
glycerone phosphate + reduced 2,6-dichlorophenylindophenol
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + 2,6-dichlorophenolindophenol
glycerone phosphate + reduced 2,6-dichlorophenylindophenol
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + 2,6-dichlorophenolindophenol
glycerone phosphate + reduced 2,6-dichlorophenylindophenol
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + acceptor
glycerone phosphate + reduced acceptor
show the reaction diagram
-
key enzyme of the glycerol phosphate shuttle
-
?
sn-glycerol 3-phosphate + acceptor
glycerone phosphate + reduced acceptor
show the reaction diagram
-
enzyme is linked to the respiratory chain
-
?
sn-glycerol 3-phosphate + coenzyme Q0
glycerone phosphate + reduced coenzyme Q0
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + coenzyme Q0
glycerone phosphate + reduced coenzyme Q0
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + coenzyme Q0
glycerone phosphate + reduced coenzyme Q0
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + coenzyme Q1
glycerone phosphate + reduced coenzyme Q1
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + coenzyme Q1
glycerone phosphate + reduced coenzyme Q1
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + coenzyme Q1
glycerone phosphate + reduced coenzyme Q1
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + coenzyme Q1
glycerone phosphate + reduced coenzyme Q1
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + coenzyme Q1
glycerone phosphate + reduced coenzyme Q1
show the reaction diagram
-
63% of the activity with 2,6-dichloroindophenol
-
?
sn-glycerol 3-phosphate + coenzyme Q10
glycerone phosphate + reduced coenzyme Q10
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + coenzyme Q10
glycerone phosphate + reduced coenzyme Q10
show the reaction diagram
-
coenzyme Q10 is the only natural electron acceptor in insect flight muscles
-
?
sn-glycerol 3-phosphate + coenzyme Q2
glycerone phosphate + reduced coenzyme Q2
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + coenzyme Q2
glycerone phosphate + reduced coenzyme Q2
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + coenzyme Q2
glycerone phosphate + reduced coenzyme Q2
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + coenzyme Q5
glycerone phosphate + reduced coenzyme Q5
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + coenzyme Q6
glycerone phosphate + reduced coenzyme Q6
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + decylubiquinone
glycerone phosphate + decylubiquinol
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + duroquinone
glycerone phosphate + duroquinol
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + ferricyanide
glycerone phosphate + ferrocyanide
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + ferricyanide
glycerone phosphate + ferrocyanide
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + ferricyanide
glycerone phosphate + ferrocyanide
show the reaction diagram
-
-
-
-
sn-glycerol 3-phosphate + ferricyanide
glycerone phosphate + ferrocyanide
show the reaction diagram
-
70% of the activity with 2,6-dichloroindophenol
-
?
sn-glycerol 3-phosphate + menadione
glycerone phosphate + reduced menadione
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + menadione
glycerone phosphate + reduced menadione
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + menadione
glycerone phosphate + reduced menadione
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + methylene blue
glycerone phosphate + reduced methylene blue
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + methylene blue
glycerone phosphate + reduced methylene blue
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + oxidized coenzyme Q
glycerone phosphate + reduced coenzyme Q
show the reaction diagram
Rattus norvegicus, Rattus norvegicus Wistar
-
-
-
?
sn-glycerol 3-phosphate + phenazine methosulfate
glycerone phosphate + reduced phenazine methosulfate
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + phenazine methosulfate
glycerone phosphate + reduced phenazine methosulfate
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + phenazine methosulfate
glycerone phosphate + reduced phenazine methosulfate
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + phenazine methosulfate
glycerone phosphate + reduced phenazine methosulfate
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + phenazine methosulfate
glycerone phosphate + reduced phenazine methosulfate
show the reaction diagram
-
phenazine methosulfate-coupled reduction of thiazolyl blue tetrazolium
-
?
sn-glycerol 3-phosphate + phenazine methosulfate
glycerone phosphate + reduced phenazine methosulfate
show the reaction diagram
-
phenazine methosulfate coupled 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide reduction assay
-
?
sn-glycerol 3-phosphate + phenazine methosulfate
glycerone phosphate + reduced phenazine methosulfate
show the reaction diagram
-
specific for L-3-glycerophosphate
-
?
sn-glycerol 3-phosphate + quinone
glycerone phosphate + quinol
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + vitamin K3
glycerone phosphate + reduced vitamin K3
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + vitamin K3
glycerone phosphate + reduced vitamin K3
show the reaction diagram
-
-
-
?
glycerol 3-phosphate + a quinone
dihydroxyacetone phosphate + a quinol
show the reaction diagram
-
-
?
additional information
?
-
-
distinct binding sites on the mitochondrial L-glycerol-3-phosphate dehydrogenase for hydrophobic and hydrophilic electron acceptors in vitro
-
-
-
additional information
?
-
-
enzyme forms part of a glycerol-3-phosphate shuttle and links cytosolic glycerol-3-phosphate metabolism to carbon source utilization and energy metabolism
-
-
-
additional information
?
-
expression of DsFAD-GPDH is enhanced at first by salt treatment, and repressed by oxygen deficiency and cold stress
-
-
-
additional information
?
-
-
high susceptibility to production of reactive oxygen species by mGPDH may reflect inefficient protection of its interaction with coenzyme Q and possibly also the low Fe-S content
-
-
-
additional information
?
-
-
SDP6 is essential for glycerol catabolism
-
-
-
SplaateNatural_Substrates,Natural_Products,id,Organism_Substrates,Commentary_Substrates,Literature_Substrates,Commentary_Products,Literature_Products,Reversibility
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
sn-glycerol 3-phosphate + acceptor
glycerone phosphate + reduced acceptor
show the reaction diagram
-
key enzyme of the glycerol phosphate shuttle
-
?
sn-glycerol 3-phosphate + acceptor
glycerone phosphate + reduced acceptor
show the reaction diagram
-
enzyme is linked to the respiratory chain
-
?
sn-glycerol 3-phosphate + coenzyme Q10
glycerone phosphate + reduced coenzyme Q10
show the reaction diagram
-
coenzyme Q10 is the only natural electron acceptor in insect flight muscles
-
?
sn-glycerol 3-phosphate + oxidized coenzyme Q
glycerone phosphate + reduced coenzyme Q
show the reaction diagram
Rattus norvegicus, Rattus norvegicus Wistar
-
-
-
?
additional information
?
-
-
enzyme forms part of a glycerol-3-phosphate shuttle and links cytosolic glycerol-3-phosphate metabolism to carbon source utilization and energy metabolism
-
-
-
additional information
?
-
Q06B39
expression of DsFAD-GPDH is enhanced at first by salt treatment, and repressed by oxygen deficiency and cold stress
-
-
-
additional information
?
-
-
high susceptibility to production of reactive oxygen species by mGPDH may reflect inefficient protection of its interaction with coenzyme Q and possibly also the low Fe-S content
-
-
-
additional information
?
-
-
SDP6 is essential for glycerol catabolism
-
-
-
SplaateCofactor,Organism,Commentary,Literature,Filename
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
coenzyme Q
-
-
FAD
-
contains 1 mol of acid-liberatable FAD per 5500000 g of protein
FAD
-
contains noncovalently bound FAD
FAD
-
contains up to 1 nmol of FAD/mg of protein
FAD
-
contains FAD as coenzyme
FAD
-
contains FAD as coenzyme
FAD
-
contains 1 mol of noncovalently bound FAD per dimer
FAD
-
contains 0.4 mol of FAD per protein monomer of 58000 Da
FAD
Q3Y0N9
dependent on
FAD
-
dependent on
flavin
-
contains 3.5 flavins per 5200000 Da molecular weight
FMN
-
contains 0.1 nmol of FMN /mg of protein
SplaateMetals_Ions,Organism,Commentary, Literature
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
0.7 mM required for half-maximal activation of activity with 2,6-dichlorophenolindophenol in euthyroid rats, 0.002 mM required for half-maximal activation of activity with vitamin K3 in euthyroid rats. Ca2+ causes an alteration in the conformation of the enzyme
Ca2+
-
activates; causes a conformational change of both the soluble and membrane-embedded enzyme
Ca2+
-
activates
Ca2+
-
activated by Ca2+
Ca2+
-
activated by Ca2+
Ca2+
lowers KM-value for glycerol phosphate
Iron
-
contains 1 gatom of acid liberatable iron per 310000 g of protein
Iron
-
contains 1 nmol non-heme iron per mg of protein
Iron
-
contains iron
Iron
-
contains 2 mol of non-heme iron per dimer
K+
-
20 mM NaCl or KCl required for maximal activity
Mg2+
-
activates
Na+
-
20 mM NaCl or KCl required for maximal activity
SplaateInhibitors, Organism, Commentary, Literature,Filename
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(NH4)2MoO4
-
-
1,10-phenanthroline
-
-
1,10-phenanthroline
-
-
1,10-phenanthroline
-
-
1,2-Dihydroxybenzene 3,5-disulfonic acid
-
-
1-(2-Thenoyl)-3,3,3-trifluoroacetone
-
-
adriamycin
-
significant inhibition of reaction with hydrophobic electron acceptors, coenzyme Q or vitamin K3
bathophenanthroline
-
-
Cetylpyridinium bromide
-
-
Chloroquine
-
inhibits reaction with coenzyme Q1, no inhibition of the reaction with 2,6-dichloroindophenol
Cu2+
-
specifically inhibits phenazine methosulfate coupled 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide reduction assay
D-2-Phosphoglyceric acid
-
-
D-3-glycerophosphate
-
-
D-3-Phosphoglyceric acid
-
-
D-3-Phosphoglyceric acid
-
competitive with respect to glycerophosphate
D-3-Phosphoglyceric acid
-
-
deoxycholate
-
-
Digitonin
-
strong inhibition, the activity is recovered to 148% of control values by the addition of exogenous synthetic analog of CoQ idebenone, i.e. hydroxydecylubiquinone, and cytochrome c. The compound also strongly activates glycerol-3-phosphate oxidation inhibited by endogenous or added free fatty acids
dihydroxyacetone phosphate
-
-
Ethacrynic acid
-
-
FMN
-
reaction with methylene blue
glyceraldehyde 3-phosphate
-
D-3-glyceraldehyde phosphate; L-3-glyceraldehyde phosphate
glyceraldehyde 3-phosphate
-
-
K3PO4
-
non-competitive
m-Chlorohydroxamate
-
-
PCMB
-
-
phosphoenolpyruvate
-
-
Phosphoglycolic acid
-
-
Salicylhydroxamate
-
-
Thenoyltrifluoroacetone
-
-
Triton X100
-
-
Triton X100
-
in presence of phospholipids
Zn2+
-
specifically inhibits ferricyanide reduction assay
SplaateActivating_Compound, Organism, Commentary, Literature,Filename
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Bathocuproine
-
slight activation
cardiolipin
-
when functioning with hydrophobic electron acceptor essential in both leaflets of the membrane
Chloroform
-
slight activation
cytochrome c
-
role of cyt c in activation of glycerol-3-phosphate and succinate oxidation in frozenthawed mitochondria
Detergent
-
required for maximal activity
-
Detergent
-
detergent-depleted enzyme requires exogenous phospholipid or nondenaturing detergent for activity
-
FAD
-
stimulates the reaction with phenazine methosulfate and methylene blue. 20% increase in activity of the reaction with phenazine methosulfate with a half-maximal concentration of 200 nM
FMN
-
stimulates the reaction with phenazine methosulfate. 6fold increase in activity with a half-maximal concentration of 0.13 mM
Phospholipid
-
detergent-depleted enzyme requires exogenous phospholipid or nondenaturing detergent for activity
sodium oleate
-
activates
SplaateKM_Value,KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.076
2,6-dichlorophenolindophenol
-
-
0.125
2,6-dichlorophenolindophenol
-
-
0.125
coenzyme Q0
-
-
0.013
coenzyme Q1
-
-
0.052
coenzyme Q1
-
-
0.05
coenzyme Q10
-
-
0.023
coenzyme Q2
-
-
0.11
coenzyme Q6
-
-
5.17
DL-alpha-glycerophosphate
-
-
-
0.8
DL-glycerol 3-phosphate
-
-
1.9
DL-glycerol 3-phosphate
-
with phenazine methosulfate coupled with 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide as electron acceptor
10.2
DL-glycerol 3-phosphate
-
with ferricyanide as electron acceptor
0.55
ferricyanide
-
-
0.1
glycerol 3-phosphate
-
in absence of exogenous FMN
0.339
glycerol 3-phosphate
-
in presence of exogenous FMN
0.4
glycerol 3-phosphate
-
enzyme from testis and pancreatic islets, in presence of saturating concentrations of free Ca2+
2.5
glycerol 3-phosphate
-
enzyme from pancreatic islets, in absence of free calcium
3.2
glycerol 3-phosphate
-
enzyme from testis in absence of free calcium
3.7
L-3-glycerophosphate
-
with ferricyanide as electron acceptor
4.02
L-3-glycerophosphate
-
with phenazine methosulfate as electron acceptor
6.2
L-3-glycerophosphate
-
with coenzyme Q10 as electron acceptor
0.13
phenazine methosulfate
-
-
10
L-3-glycerophosphate
-
with 2,6-dichloroindophenol, phenazine methosulfate, coenzyme Q0, coenzyme Q1, coenzyme Q2 or coenzyme Q6 as electron acceptor
additional information
additional information
-
-
-
SplaateTurnover_Number, Turnover_Number_Maximum, Substrate,Organism,Commentary, Literature, Filename
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
33.3
glycerol 3-phosphate
Escherichia coli
-
-
SplaateKCat_KM_Value,KCat_KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
SplaateKI_Value,KI_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
SplaateIC50_Value,IC50_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
SplaateSpecific_Activity, Specific_Activity_Maximum, Organism ,Commentary, Literature
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
2.9
-
-
additional information
-
-
additional information
-
-
SplaatepH_Optimum, pH_Optimum_Maximum, Organism, Commentary, Literature
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7 - 9.5
-
-
7.4
-
assay at
SplaatepH_Range,pH_Range_Maximum, Organism,Commentary, Literature
SplaateTemperature_Optimum, Temperature_Optimum_Maximum, Organism, Commentary, Literature
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
-
assay at
SplaateTemperature_Range, Temperature_Range_Maximum, Organism, Commentary, Literature
SplaatepI_Value,pI_Value_Maximum, Organism,Commentary, Literature
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.7 - 7.1
-
isoelectric focusing
SplaateSource_Tissue, Organism, Commentary, Literature, Textmining
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
glycerol-3-phosphate dehydrogenase protein levels and enzyme activity are highest in brown adipose tissue
Manually annotated by BRENDA team
-
very low activity
Manually annotated by BRENDA team
Rattus norvegicus Wistar
-
very low activity
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar
-
-
-
Manually annotated by BRENDA team
SplaateLocalization, Organism, Commentary, id_go, Literature, Textmining
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
associated with membrane
Manually annotated by BRENDA team
Rattus norvegicus Wistar
-
-
-
Manually annotated by BRENDA team
-
inner mitochondrial membrane. The mature protein has three transmembrane helices. The first membrane-spanning region coincides with the FAD site and thus this site is placed within the membrane. The calcium-binding region lies outside the membrane exposed to the cytosolic environment
Manually annotated by BRENDA team
-
nuclear-encoded enzyme associated with the inner mitochondrial membrane
Manually annotated by BRENDA team
-
tightly bound to the outer surface of the inner mitochondrial membrane
Manually annotated by BRENDA team
-
located on the inner mitochondrial membrane
Manually annotated by BRENDA team
-
the enzyme is localized to the outer membrane of the mitochondria facing the cytoplasm
Manually annotated by BRENDA team
SplaatePDB,PDB,PDB,Organism,Uniprot_ID
PDB
SCOP
CATH
ORGANISM
UNIPROT
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
SplaateMolecular_Weight, Molecular_Weight_Maximum, Organism, Commentary, Literature
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
80000
-
gel filtration
287813
80000
-
SDS-PAGE
713077
93500
-
equilibrium sedimentation
287817
130000
-
gel filtration
287818
300000
-
gel filtration
287807
520000
-
non-denaturing PAGE
287805
SplaateSubunits, Organism, Commentary, Literature
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 75000, SDS-PAGE
?
-
x * 68000, SDS-PAGE
dimer
-
2 * 35000, SDS-PAGE
dimer
-
1 * 43000 + 1 * 62000, SDS-PAGE
dimer
-
2 * 58000, SDS-PAGE
SplaatePosttranslational_Modification, Organism, Commentary, Literature
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phosphoprotein
-
GPD2 is tyrosine phosphorylated only in capacitated spermatozoa of wild type mice
lipoprotein
-
very low phospholipid content
SplaateCommentary, Organism, Literature
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
structure of a deletion mutant of Streptococcus sp. GlpO (GlpODELTA, lacking a 50-residue insert that includes a flexible surface region) is determined using multiwavelength anomalous dispersion data and refined at 2.3 A resolution
Q3Y0N9
SplaatepH_Stability,pH_Stability_Maximum, Organism, Commentary, Literature
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.5
-
unstable below
287806
SplaateTemperature_Stability,Temperature_Stability_Maximum, Organism, Commentary, Literature
SplaateGeneral_Stability, Organism, Literature
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
unstable to repeated freezing and thawing
-
SplaateOrganic_Solvent, Organism, Commentary, Literature
SplaateOxidation_Stability,Organism,Literature
SplaateStorage_Stability, Organism, Literature
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70C or 4C, concentrated to 4-10 mg/ml, stable
-
-70C, 30% ethylene glycol, stable for at least 1 month
-
3C, 30% ethylene glycol, stable for at least 4 days
-
4C, pH 6.0-8.5, 20% ethylene glycol v/v, 10% loss of activity after 60 h
-
SplaateCommentary, Organism, Literature
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
deletion mutant of Streptococcus sp. GlpO (GlpODELTA, lacking a 50-residue insert that includes a flexible surface region)
Q3Y0N9
partial
-
SplaateCommentary, Organism, Literature
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
deletion mutant of Streptococcus sp. GlpO (GlpODELTA, lacking a 50-residue insert that includes a flexible surface region) is expressed in Escherichia coli
Q3Y0N9
SplaateCommentary, Organism, Literature
SplaateEngineering, Organism, Commentary, Literature
SplaateCommentary, Organism, Literature
SplaateApplication,Organism,Commentary,Literature