Information on EC 1.1.1.B28 - dTDP-4-dehydro-6-deoxyglucose reductase [NAD(P)H]

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The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
1.1.1.B28
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
dTDP-4-dehydro-6-deoxyglucose reductase [NAD(P)H]
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dTDP-alpha-D-fucopyranose + NAD(P)+ = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NAD(P)H + H+
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
dTDP-alpha-D-fucopyranose:NAD(P)+ oxidoreductase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dTDP-alpha-D-fucopyranose + NADH + H+
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NAD+
show the reaction diagram
dTDP-alpha-D-fucopyranose + NADP+
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+
show the reaction diagram
dTDP-alpha-D-fucopyranose + NADPH + H+
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADP+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dTDP-alpha-D-fucopyranose + NADP+
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
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NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH
NADPH
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NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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Fcf1 is a divalent cation-independent enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Co2+
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5 mM, 11% inhibition
Cu2+
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5 mM, 88% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.38
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
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37C, pH 7.4
0.84
NADH
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37C, pH 7.4
0.89
NADPH
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37C, pH 7.4
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.78
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
Escherichia coli
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37C, pH 7.4
26.67
NADH
Escherichia coli
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37C, pH 7.4
6.76
NADPH
Escherichia coli
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37C, pH 7.4
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.4
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
Escherichia coli
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37C, pH 7.4
6388
31.75
NADH
Escherichia coli
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37C, pH 7.4
8
7.6
NADPH
Escherichia coli
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37C, pH 7.4
5
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 37
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 50
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4C: 47% of maximal activity, 50C: 36% of maximal activity
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40700
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x * 40700, His6-tagged fusion protein, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, in 50% glycerol, stable for at least 5 months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
His6-tagged fusion protein
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
overexpression in Escherichia coli BL21
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