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Information on EC 1.1.1.49 - glucose-6-phosphate dehydrogenase (NADP+) and Organism(s) Plasmodium falciparum

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IUBMB Comments
The enzyme catalyses a step of the pentose phosphate pathway. The enzyme is specific for NADP+. cf. EC 1.1.1.363, glucose-6-phosphate dehydrogenase [NAD(P)+] and EC 1.1.1.388, glucose-6-phosphate dehydrogenase (NAD+).
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This record set is specific for:
Plasmodium falciparum
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The taxonomic range for the selected organisms is: Plasmodium falciparum
The enzyme appears in selected viruses and cellular organisms
Synonyms
g6pd, glucose-6-phosphate dehydrogenase, g6pdh, g-6-pd, glucose 6-phosphate dehydrogenase, g-6-pdh, g-6pd, zwf-1, glucose-6-phosphate 1-dehydrogenase, glc6pdh, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6-phosphoglucose dehydrogenase
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D-glucose 6-phosphate dehydrogenase
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Entner-Doudoroff enzyme
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G6PD
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G6PD1
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G6PD2
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G6PD3
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G6PD4
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G6PD5
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G6PD6
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G6PDH1
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G6PDH2
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G6PDH3
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G6PDH4
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G6PDH5
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G6PDH6
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glucose 6-phosphate dehydrogenase (NADP)
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glucose-6-phosphate 1-dehydrogenase
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glucose-6-phosphate dehydrogenase
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glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase
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NADP-dependent glucose 6-phophate dehydrogenase
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NADP-glucose-6-phosphate dehydrogenase
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VEG11
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Vegetative protein 11
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Zwischenferment
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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SYSTEMATIC NAME
IUBMB Comments
D-glucose-6-phosphate:NADP+ 1-oxidoreductase
The enzyme catalyses a step of the pentose phosphate pathway. The enzyme is specific for NADP+. cf. EC 1.1.1.363, glucose-6-phosphate dehydrogenase [NAD(P)+] and EC 1.1.1.388, glucose-6-phosphate dehydrogenase (NAD+).
CAS REGISTRY NUMBER
COMMENTARY hide
9001-40-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
show the reaction diagram
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?
additional information
?
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the enzyme is a bifunctional enzyme glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase, the enzyme activity is analyzed for the reaction of glucose-6-phosphate dehydrogenase, EC 1.1.1.49, in a coupled assay with diaphorase activity, which uses NADPH to reduce resazurin to form the highly fluorescent molecule resorufin
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
show the reaction diagram
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?
additional information
?
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the enzyme is a bifunctional enzyme glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase, the enzyme activity is analyzed for the reaction of glucose-6-phosphate dehydrogenase, EC 1.1.1.49, in a coupled assay with diaphorase activity, which uses NADPH to reduce resazurin to form the highly fluorescent molecule resorufin
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-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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required
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(4Z)-4-(3-bromobenzylidene)-1-(4-methylphenyl)pyrazolidine-3,5-dione
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(5Z)-1-(4-bromophenyl)-5-[[5-(2-nitrophenyl)furan-2-yl]methylidene]pyrimidine-2,4,6(1H,3H,5H)-trione
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(5Z)-1-(4-ethoxyphenyl)-5-[[5-(2-nitrophenyl)furan-2-yl]methylidene]pyrimidine-2,4,6(1H,3H,5H)-trione
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(5Z)-5-[[5-(2-methyl-4-nitrophenyl)furan-2-yl]methylidene]-1-(3-methylphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
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reversible, non-competitive inhibition versus NADP+, mixed-type versus D-glucose 6-phosphate
2-[(5Z)-5-(4-hydroxy-3-methoxybenzylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]-3-phenylpropanoate
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2-[(E)-(4-nitrocyclohexa-2,4-dien-1-yl)(2-phenylhydrazinylidene)methyl]benzene-1,4-diol
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non-competitive inhibition versus NADP+, non-competitive or mixed-type versus D-glucose 6-phosphate
2-[4-(4-chlorophenyl)-1,3-thiazol-2-yl]-5-methyl-4-(3,4,5-trimethoxybenzyl)-2,4-dihydro-3H-pyrazol-3-one
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irreversible, non-competitive inhibition versus NADP+, mixed-type versus D-glucose 6-phosphate
3-(4-hydroxyphenyl)-2-(pyridin-3-yl)quinazolin-4(3H)-one
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irreversible, non-competitive inhibition versus NADP+, mixed-type versus D-glucose 6-phosphate
3-(5-bromopyridin-2-yl)-2-(pyridin-3-yl)quinazolin-4(3H)-one
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irreversible, non-competitive inhibition versus NADP+, mixed-type versus D-glucose 6-phosphate
3-(5-[(E)-[2-(5-nitropyridin-2-yl)hydrazinylidene]methyl]furan-2-yl)benzoic acid
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4-fluoro-N-(4-hydroxynaphthalen-1-yl)benzenesulfonamide
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4-methyl-3-(propan-2-yloxy)-6H-benzo[c]chromen-6-one
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non-competitive inhibition versus NADP+, mixed-type versus D-glucose 6-phosphate
5-[[5-(4-methoxy-2-nitrophenyl)furan-2-yl]methylidene]-2-thioxodihydropyrimidine-4,6(1H,5H)-dione
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reversible, competitive inhibition versus NADP+, mixed-type versus D-glucose 6-phosphate
ellagic acid
mixed type inhibitor of glucose 6-phosphate activity
N-(4-hydroxynaphthalen-1-yl)-2,5-dimethylbenzenesulfonamide
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additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0145
D-glucose 6-phosphate
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pH 7.5, temperature not specified in the publication
0.0086
NADP+
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pH 7.5, temperature not specified in the publication
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0018
2-[(E)-(4-nitrocyclohexa-2,4-dien-1-yl)(2-phenylhydrazinylidene)methyl]benzene-1,4-diol
Plasmodium falciparum
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pH 7.5, temperature not specified in the publication
0.0039
4-fluoro-N-(4-hydroxynaphthalen-1-yl)benzenesulfonamide
Plasmodium falciparum
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pH 7.5, temperature not specified in the publication
0.00097
4-methyl-3-(propan-2-yloxy)-6H-benzo[c]chromen-6-one
Plasmodium falciparum
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pH 7.5, temperature not specified in the publication
0.000076
ellagic acid
Plasmodium falciparum
pH 7.5, 25°C
0.0053
N-(4-hydroxynaphthalen-1-yl)-2,5-dimethylbenzenesulfonamide
Plasmodium falciparum
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pH 7.5, temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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the bifunctional glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase enzyme catalyzes the first and second steps in the parasite's pentose phosphate pathway, cf. EC 3.1.1.31
physiological function
the enzyme is essential for the growth of blood stage parasites
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q27741_PLAFA
910
0
106987
TrEMBL
other Location (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 109000, calculated from sequence, x * 100000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
no significant upregulation of expression in presence of artemisinin, chloroquine or methylene blue
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
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the enzyme is a target for anti-parasite drug development
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yoshida, A.; Roth, E.F.
Glucose-6-phosphate dehydrogenase of malaria parasite Plasmodium falciparum
Blood
69
1528-1530
1987
Plasmodium falciparum
Manually annotated by BRENDA team
Preuss, J.; Hedrick, M.; Sergienko, E.; Pinkerton, A.; Mangravita-Novo, A.; Smith, L.; Marx, C.; Fischer, E.; Jortzik, E.; Rahlfs, S.; Becker, K.; Bode, L.
High-throughput screening for small-molecule inhibitors of Plasmodium falciparum glucose-6-phosphate dehydrogenase 6-phosphogluconolactonase
J. Biomol. Screen.
17
738-751
2012
Plasmodium falciparum
Manually annotated by BRENDA team
Allen, S.M.; Lim, E.E.; Jortzik, E.; Preuss, J.; Chua, H.H.; MacRae, J.I.; Rahlfs, S.; Haeussler, K.; Downton, M.T.; McConville, M.J.; Becker, K.; Ralph, S.A.
Plasmodium falciparum glucose-6-phosphate dehydrogenase 6-phosphogluconolactonase is a potential drug target
FEBS J.
282
3808-3823
2015
Plasmodium falciparum (Q8IKU0), Plasmodium falciparum
Manually annotated by BRENDA team