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Information on EC 1.1.1.49 - glucose-6-phosphate dehydrogenase (NADP+) and Organism(s) Arabidopsis thaliana
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1.1.1.49 glucose-6-phosphate dehydrogenase (NADP+)IUBMB Comments
The enzyme catalyses a step of the pentose phosphate pathway. The enzyme is specific for NADP+. cf. EC 1.1.1.363, glucose-6-phosphate dehydrogenase [NAD(P)+] and EC 1.1.1.388, glucose-6-phosphate dehydrogenase (NAD+).
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Word Map
- 1.1.1.49
- erythrocyte
- pentose
-
hemolytic
- catalase
- malaria
- 6-phosphogluconate
- anemia
- dismutase
- neonatal
- hexokinase
- hemoglobin
- gsh
-
malic
- newborn
- plasmodium
- children
-
haemolysis
- glycogen
- malate
- jaundice
-
x-linked
- sod
- falciparum
- isocitrate
- hyperbilirubinemia
-
lipogenic
-
sickle
- infant
- bilirubin
-
shunt
-
mediterranean
-
endemic
-
lipogenesis
- glucose-6-phosphatase
- vivax
-
phosphofructokinase
- transfusion
- glutathione-s-transferase
-
cytochemical
- dehydroepiandrosterone
- glucokinase
-
thiobarbituric
- hexose
- phosphoglucomutase
- methemoglobin
- thalassemia
- hemoglobinopathy
-
phototherapy
-
x-chromosome
- synthesis
- drug development
- medicine
- industry
- beta-thalassemia
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
g6pd, glucose-6-phosphate dehydrogenase, g6pdh, g-6-pd, glucose 6-phosphate dehydrogenase, g-6-pdh, g-6pd, zwf-1, glucose-6-phosphate 1-dehydrogenase, glc6pdh, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
6-phosphoglucose dehydrogenase
-
-
-
-
D-glucose 6-phosphate dehydrogenase
-
-
-
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Entner-Doudoroff enzyme
-
-
-
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G6PD
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-
-
-
G6PD1
G6PD2
G6PD3
G6PD4
G6PD5
G6PD6
G6PDH
G6PDH1
G6PDH2
-
-
-
-
G6PDH3
-
-
-
-
G6PDH4
-
-
-
-
G6PDH5
-
-
-
-
G6PDH6
-
-
-
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glucose 6-phosphate dehydrogenase (NADP)
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-
-
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glucose-6-phosphate 1-dehydrogenase
-
-
-
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glucose-6-phosphate dehydrogenase
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-
-
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NADP-dependent glucose 6-phophate dehydrogenase
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-
-
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NADP-glucose-6-phosphate dehydrogenase
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-
-
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VEG11
-
-
-
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Vegetative protein 11
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-
-
-
Zwischenferment
-
-
-
-
G6PD1
-
-
-
-
G6PD2
-
-
-
-
G6PD3
-
-
-
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G6PD4
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-
-
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G6PD5
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-
-
-
G6PD6
-
-
-
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G6PDH1
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
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redox reaction
-
-
-
-
reduction
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-
-
-
PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
-
-, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
D-glucose-6-phosphate:NADP+ 1-oxidoreductase
The enzyme catalyses a step of the pentose phosphate pathway. The enzyme is specific for NADP+. cf. EC 1.1.1.363, glucose-6-phosphate dehydrogenase [NAD(P)+] and EC 1.1.1.388, glucose-6-phosphate dehydrogenase (NAD+).
CAS REGISTRY NUMBER
COMMENTARY
9001-40-5
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
G6PDH plays a role in supplying NADPH for oil accumulation in developing seeds in which photosynthesis may be light limited
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
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-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
Trx f1 regulates G6PDH1 activity as efficiently as Trx m1 or m4. Trx x is a very poor regulator of G6PDH activity. Trx y1 is inefficient as inhibitor but it shows high efficiency in activation. Upon illumination, a strong and fast reductive inhibition of G6PDH1 activity dependent on the presence of all the components of the Fd/Trx system
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additional information
during 7 days of phosphate starvation, G6PD5 is continuously expressed throughout phosphate-starvation; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
during 7 days of phosphate starvation, G6PD5 is continuously expressed throughout phosphate-starvation; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
during 7 days of phosphate starvation, G6PD5 is continuously expressed throughout phosphate-starvation; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
during 7 days of phosphate starvation, G6PD5 is continuously expressed throughout phosphate-starvation; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
during 7 days of phosphate starvation, G6PD5 is continuously expressed throughout phosphate-starvation; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
during 7 days of phosphate starvation, G6PD5 is continuously expressed throughout phosphate-starvation; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
-
during 7 days of phosphate starvation, G6PD5 is continuously expressed throughout phosphate-starvation; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
sucrose
-
in wild-type seedlings G6PD5 and G6PD6 show small (1.7- and 2fold, respectively) but significant induction by sucrose
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
-
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
oxidative activation is strictly dependent on plastidial thioredoxins that show differential efficiencies. Trx f1 regulates G6PDH1 activity as efficiently as Trx m1 or m4. Trx x is a very poor regulator of G6PDH activity. Trx y1 is inefficient as inhibitor but it shows high efficiency in activation. G6PDH activity can be recovered by transferring from light to dark, reversibility of inactivation certifying redox modulation of the enzyme. In the dark, reactivation can be enhanced by the addition of oxidants, such as oxidized dithiothreitol and H2O2
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
additional information
additional information
the levels of transcripts are low at day 0 and increase markedly at day 1, then gradually decrease
-
additional information
the levels of transcripts are low at day 0 and increase markedly at day 1, then gradually decrease
-
additional information
the levels of transcripts are low at day 0 and increase markedly at day 1, then gradually decrease
-
additional information
the levels of transcripts are low at day 0 and increase markedly at day 1, then gradually decrease
-
additional information
the levels of transcripts are low at day 0 and increase markedly at day 1, then gradually decrease
-
additional information
the levels of transcripts are low at day 0 and increase markedly at day 1, then gradually decrease
-
additional information
-
the levels of transcripts are low at day 0 and increase markedly at day 1, then gradually decrease
-
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). G6PD1_ARATH
576
0
65428
Swiss-Prot
Chloroplast (Reliability: 1)
G6PD2_ARATH
596
0
67160
Swiss-Prot
Chloroplast (Reliability: 2)
G6PD3_ARATH
599
0
67358
Swiss-Prot
Chloroplast (Reliability: 2)
G6PD5_ARATH
516
0
59157
Swiss-Prot
other Location (Reliability: 2)
G6PD6_ARATH
515
0
59116
Swiss-Prot
other Location (Reliability: 3)
A0A654E8G0_ARATH
625
0
70277
TrEMBL
Chloroplast (Reliability: 1)
A0A7G2DSV4_ARATH
599
0
67384
TrEMBL
Chloroplast (Reliability: 2)
A0A5S9Y8D8_ARATH
575
0
65359
TrEMBL
Chloroplast (Reliability: 1)
A0A654FI00_ARATH
516
0
59131
TrEMBL
other Location (Reliability: 2)
Q0WSL0_ARATH
515
0
59116
TrEMBL
other Location (Reliability: 3)
A0A178UHI0_ARATH
596
0
67160
TrEMBL
Chloroplast (Reliability: 2)
A0A5S9YAV9_ARATH
483
0
55362
TrEMBL
other Location (Reliability: 3)
A0A1I9LLZ6_ARATH
587
2
67479
TrEMBL
Secretory Pathway (Reliability: 2)
A0A7G2FEC3_ARATH
881
0
101011
TrEMBL
other Location (Reliability: 3)
A0A178W6L2_ARATH
625
0
70196
TrEMBL
Chloroplast (Reliability: 1)
A0A178UEB2_ARATH
578
0
65532
TrEMBL
Chloroplast (Reliability: 1)
Q5E919_ARATH
516
0
59157
TrEMBL
other Location (Reliability: 2)
A0A5S9Y423_ARATH
596
0
67146
TrEMBL
Chloroplast (Reliability: 2)
A0A5S9TFV9_ARATH
625
0
70202
TrEMBL
Chloroplast (Reliability: 2)
A0A654G5D7_ARATH
576
0
65413
TrEMBL
Chloroplast (Reliability: 1)
A0A384LPG4_ARATH
515
0
59116
TrEMBL
other Location (Reliability: 3)
F4I0Y8_ARATH
635
0
71468
TrEMBL
Chloroplast (Reliability: 2)
Q56WK7_ARATH
364
0
41679
TrEMBL
Mitochondrion (Reliability: 5)
A0A1I9LLZ7_ARATH
570
0
65604
TrEMBL
Secretory Pathway (Reliability: 5)
A0A178VBL3_ARATH
516
0
59131
TrEMBL
other Location (Reliability: 2)
A0A178WP00_ARATH
599
0
67358
TrEMBL
Chloroplast (Reliability: 2)
A0A178UCB6_ARATH
435
0
50000
TrEMBL
other Location (Reliability: 2)
Q0WPI4_ARATH
625
0
70202
TrEMBL
Chloroplast (Reliability: 2)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
activities of the cytosolic isoforms G6PD5 and G6PD6 are reciprocally increased in single mutants with no increase of their respective transcript levels. Seeds of the double mutant but not of the single mutants have higher oil content and increased weight compared to those of the wild-type, with no alteration in the carbon to nitrogen ratio or fatty acid composition. Total G6PDH activity is reduced only in the double mutant
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli DH5alpha strain from the pASK-G6PDH1 plasmid
into vector pET16b and expressed in a G6PDH-deficient Escherichia coli strain. Overexpression in the cytosol of the susceptible tobacco cultivar Xanthi
transient expression of the G6PD5 and G6PD6 fused to a GFP gene, cloned into the SpeI site of pCAMBIA1302. T-DNA insertion lines transformed with cosmid clones containing the regions of G6PD5 and G6PD6 isolated from a genomic cosmid (pBIC20) library
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
because isoenzyme replacement of G6PDH in the cytosol of tobacco is beneficial under various kinds of cues, this strategy may be a tool to enhance stress tolerance in general
additional information
-
loss of cytosolic G6PDH activity affects the metabolism of developing seeds by increasing carbon substrates for synthesis of storage compounds rather than by decreasing the NADPH supply specifically for fatty acid synthesis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nee, G.; Zaffagnini, M.; Trost, P.; Issakidis-Bourguet, E.
Redox regulation of chloroplastic glucose-6-phosphate dehydrogenase: a new role for f-type thioredoxin
FEBS Lett.
583
2827-2832
2009
Arabidopsis thaliana (Q43727)
Wakao, S.; Andre, C.; Benning, C.
Functional analyses of cytosolic glucose-6-phosphate dehydrogenases and their contribution to seed oil accumulation in arabidopsis
Plant Physiol.
146
277-288
2008
Arabidopsis thaliana
Scharte, J.; Schoen, H.; Tjaden, Z.; Weis, E.; von Schaewen, A.
Isoenzyme replacement of glucose-6-phosphate dehydrogenase in the cytosol improves stress tolerance in plants
Proc. Natl. Acad. Sci. USA
106
8061-8066
2009
Nicotiana tabacum, Arabidopsis thaliana (Q8L743)
Yin, Y.; Ashihara, H.
Expression of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase isoform genes in suspension-cultured Arabidopsis thaliana cells
Z. Naturforsch. C
63
713-720
2008
Arabidopsis thaliana (Q43727), Arabidopsis thaliana (Q8L743), Arabidopsis thaliana (Q93ZW0), Arabidopsis thaliana (Q9FJI5), Arabidopsis thaliana (Q9FY99), Arabidopsis thaliana (Q9LK23), Arabidopsis thaliana, Arabidopsis thaliana T87 (Q43727), Arabidopsis thaliana T87 (Q8L743), Arabidopsis thaliana T87 (Q93ZW0), Arabidopsis thaliana T87 (Q9FJI5), Arabidopsis thaliana T87 (Q9FY99), Arabidopsis thaliana T87 (Q9LK23)
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