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Information on EC 1.1.1.41 - isocitrate dehydrogenase (NAD+) and Organism(s) Rattus norvegicus

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EC Tree
IUBMB Comments
Requires Mn2+ or Mg2+ for activity. Unlike EC 1.1.1.42, isocitrate dehydrogenase (NADP+), oxalosuccinate cannot be used as a substrate. In eukaryotes, isocitrate dehydrogenase exists in two forms: an NAD+-linked enzyme found only in mitochondria and displaying allosteric properties, and a non-allosteric, NADP+-linked enzyme that is found in both mitochondria and cytoplasm . The enzyme from some species can also use NADP+ but much more slowly .
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Rattus norvegicus
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The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Reaction Schemes
hide
isocitrate
+
NAD+
=
2-oxoglutarate
+
CO2
+
NADH
+
H+
+
=
+
+
+
Synonyms
isocitrate dehydrogenase, isocitrate dehydrogenase 2, isocitric dehydrogenase, nad-dependent isocitrate dehydrogenase, idh3a, nad-idh, nad-isocitrate dehydrogenase, nad-icdh, nad+-dependent isocitrate dehydrogenase, nad-linked isocitrate dehydrogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
beta-ketoglutaric-isocitric carboxylase
-
-
-
-
IDH1
-
-
-
-
isocitrate dehydrogenase 3alpha subunit
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isocitric acid dehydrogenase
-
-
-
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isocitric dehydrogenase
-
-
-
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NAD dependent isocitrate dehydrogenase
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-
-
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NAD isocitrate dehydrogenase
-
-
-
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NAD isocitric dehydrogenase
-
-
-
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NAD+-dependent isocitrate dehydrogenase 3
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NAD+-specific ICDH
-
-
-
-
NAD-linked isocitrate dehydrogenase
-
-
-
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NAD-specific isocitrate dehydrogenase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
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oxidative decarboxylation
-
-
-
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redox reaction
-
-
-
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reduction
-
-
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reductive carboxylation
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
isocitrate:NAD+ oxidoreductase (decarboxylating)
Requires Mn2+ or Mg2+ for activity. Unlike EC 1.1.1.42, isocitrate dehydrogenase (NADP+), oxalosuccinate cannot be used as a substrate. In eukaryotes, isocitrate dehydrogenase exists in two forms: an NAD+-linked enzyme found only in mitochondria and displaying allosteric properties, and a non-allosteric, NADP+-linked enzyme that is found in both mitochondria and cytoplasm [7]. The enzyme from some species can also use NADP+ but much more slowly [9].
CAS REGISTRY NUMBER
COMMENTARY hide
9001-58-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
isocitrate + NAD+
2-oxoglutarate + CO2 + NADH + H+
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
isocitrate + NAD+
2-oxoglutarate + CO2 + NADH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
ubiquitous tissue specific expression of isocitrate dehydrogenase 3alpha subunit
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
overexpression of IDH3alpha increases the phosphorylation level of Akt (protein kinase B) and neutralizes the cellular toxicity induced by aflatoxin B1 (AFB1) or H2O2 and apoptosis induced by AFB1, while the reduced expression of IDH3alpha by siRNA decreases the phosphorylation, indicating that IDH3alpha plays important roles in the oxidative stress-induced PI3K/Akt pathway. AFB1 treatment can increase the expression of IDH3alpha, and the activated PI3K/Aktpathway by IDH3alpha eventually neutralizes the apoptosis induced by AFB1
physiological function
IDH3alpha increases the phosphorylation level and activation of Akt (protein kinase B)
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
IDH3A_RAT
366
0
39614
Swiss-Prot
Mitochondrion (Reliability: 3)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
enzyme overexpression, or enzyme downregulation by siRNA
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
IDH3alpha, DNA and amino acid sequence analysis and comparison
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
aflatoxin B1, AFB1, upregulates the enzyme in vivo and in vitro, in cells and in the process of AFB1-induced liver lesion
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Plaut, G.W.E.
Isocitrate dehydrogenase
The Enzymes, 2nd Ed. (Boyer, P. D. , Lardy, H. , Myrbck, K. , eds. )
7
105-126
1963
Aspergillus niger, Bos taurus, Saccharomyces cerevisiae, Cavia porcellus, Columba livia, Homo sapiens, Pisum sativum, Rattus norvegicus
-
Manually annotated by BRENDA team
Rutter, G.A.; Denton, R.M.
Regulation of NAD+-linked isocitrate dehydrogenase and 2-oxoglutarate dehydrogenase by Ca2+ ions within toluene-permeabilized rat heart mitochondria. Interactions with regulation by adenine nucleotides and NADH/NAD+ ratios [published erratum appears in Biochem J 1988 Aug 1;253(3):935]
Biochem. J.
252
181-189
1988
Rattus norvegicus
Manually annotated by BRENDA team
Yang, C.; Fan, J.; Zhuang, Z.; Fang, Y.; Zhang, Y.; Wang, S.
The role of NAD+-dependent isocitrate dehydrogenase 3 subunit alpha in AFB1 induced liver lesion
Toxicol. Lett.
224
371-379
2014
Homo sapiens (P50213), Rattus norvegicus (Q99NA5), Mus musculus (Q9D6R2)
Manually annotated by BRENDA team