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Information on EC 1.1.1.41 - isocitrate dehydrogenase (NAD+) and Organism(s) Arabidopsis thaliana

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EC Tree
IUBMB Comments
Requires Mn2+ or Mg2+ for activity. Unlike EC 1.1.1.42, isocitrate dehydrogenase (NADP+), oxalosuccinate cannot be used as a substrate. In eukaryotes, isocitrate dehydrogenase exists in two forms: an NAD+-linked enzyme found only in mitochondria and displaying allosteric properties, and a non-allosteric, NADP+-linked enzyme that is found in both mitochondria and cytoplasm . The enzyme from some species can also use NADP+ but much more slowly .
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Arabidopsis thaliana
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Reaction Schemes
Synonyms
isocitrate dehydrogenase, isocitrate dehydrogenase 2, isocitric dehydrogenase, nad-dependent isocitrate dehydrogenase, idh3a, nad-idh, nad-isocitrate dehydrogenase, nad-icdh, nad+-dependent isocitrate dehydrogenase, nad-linked isocitrate dehydrogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
beta-ketoglutaric-isocitric carboxylase
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-
-
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isocitric acid dehydrogenase
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-
-
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isocitric dehydrogenase
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-
-
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NAD dependent isocitrate dehydrogenase
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-
-
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NAD isocitrate dehydrogenase
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-
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NAD isocitric dehydrogenase
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-
-
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NAD+-dependent isocitrate dehydrogenase
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NAD+-specific ICDH
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-
-
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NAD-dependent isocitrate dehydrogenase
NAD-linked isocitrate dehydrogenase
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-
-
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NAD-specific isocitrate dehydrogenase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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oxidative decarboxylation
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-
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redox reaction
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-
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reduction
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reductive carboxylation
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-
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SYSTEMATIC NAME
IUBMB Comments
isocitrate:NAD+ oxidoreductase (decarboxylating)
Requires Mn2+ or Mg2+ for activity. Unlike EC 1.1.1.42, isocitrate dehydrogenase (NADP+), oxalosuccinate cannot be used as a substrate. In eukaryotes, isocitrate dehydrogenase exists in two forms: an NAD+-linked enzyme found only in mitochondria and displaying allosteric properties, and a non-allosteric, NADP+-linked enzyme that is found in both mitochondria and cytoplasm [7]. The enzyme from some species can also use NADP+ but much more slowly [9].
CAS REGISTRY NUMBER
COMMENTARY hide
9001-58-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-isocitrate + NAD+
2-oxoglutarate + CO2 + NADH
show the reaction diagram
-
-
-
?
isocitrate + NAD+
2-oxoglutarate + CO2 + NADH + H+
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
isocitrate + NAD+
2-oxoglutarate + CO2 + NADH + H+
show the reaction diagram
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key enzyme in the Krebs cycle
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.092
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partially purified enzyme, idh-II mutant
0.169
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partially purified enzyme, wild-type
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
from liquid shaker cultures
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
IDH5_ARATH
374
0
40625
Swiss-Prot
Mitochondrion (Reliability: 2)
IDH6_ARATH
374
0
40576
Swiss-Prot
Mitochondrion (Reliability: 5)
IDH3_ARATH
368
0
39957
Swiss-Prot
-
IDH2_ARATH
367
0
39590
Swiss-Prot
-
IDH1_ARATH
367
0
39627
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32850
IDH IV, without the predicted mitochondrial target peptide, calculated from sequence of amino acids
35910
IDH III, without the predicted mitochondrial target peptide, calculated from sequence of amino acids
36040
IDH VI, without the predicted mitochondrial target peptide, calculated from sequence of amino acids
36200
IDH V, without the predicted mitochondrial target peptide, calculated from sequence of amino acids
36810
IDH II, without the predicted mitochondrial target peptide, calculated from sequence of amino acids
38900
IDH I, without the predicted mitochondrial target peptide, calculated from sequence of amino acids
39590
IDH II, with the predicted mitochondrial target peptide, calculated from sequence of amino acids
39630
IDH I, with the predicted mitochondrial target peptide, calculated from sequence of amino acids
39960
IDH III, with the predicted mitochondrial target peptide, calculated from sequence of amino acids
40580
IDH VI, with the predicted mitochondrial target peptide, calculated from sequence of amino acids
40620
IDH V, with the predicted mitochondrial target peptide, calculated from sequence of amino acids
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
subunit IDH-I can substitute for a deficient mutant subunit IDH-II
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
mitochondrial preparation
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lin, M.; Behal, R.H.; Oliver, D.J.
Characterization of a mutation in the IDH-II subunit of the NAD+-dependent isocitrate dehydrogenase from Arabidopsis thaliana
Plant Sci.
166
983-988
2004
Arabidopsis thaliana, Arabidopsis thaliana Landsberg
Manually annotated by BRENDA team
Lemaitre, T.; Hodges, M.
Expression analysis of Arabidopsis thaliana NAD-dependent isocitrate dehydrogenase genes shows the presence of a functional subunit that is mainly expressed in the pollen and absent from vegetative organs
Plant Cell Physiol.
47
634-643
2006
Arabidopsis thaliana, Arabidopsis thaliana (O81796), Arabidopsis thaliana (P93032), Arabidopsis thaliana (Q8LFC0), Arabidopsis thaliana (Q8LG77), Arabidopsis thaliana (Q945K7)
Manually annotated by BRENDA team
Lemaitre, T.; Urbanczyk-Wochniak, E.; Flesch, V.; Bismuth, E.; Fernie, A.R.; Hodges, M.
NAD-dependent isocitrate dehydrogenase mutants of Arabidopsis suggest the enzyme is not limiting for nitrogen assimilation
Plant Physiol.
144
1546-1558
2007
Arabidopsis thaliana (P93032), Arabidopsis thaliana (Q8LFC0), Arabidopsis thaliana (Q945K7)
Manually annotated by BRENDA team