Information on EC 1.1.1.38 - malate dehydrogenase (oxaloacetate-decarboxylating)

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.1.1.38
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RECOMMENDED NAME
GeneOntology No.
malate dehydrogenase (oxaloacetate-decarboxylating)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(S)-malate + NAD+ = pyruvate + CO2 + NADH
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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oxidative decarboxylation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
anaerobic energy metabolism (invertebrates, mitochondrial)
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C4 photosynthetic carbon assimilation cycle, NAD-ME type
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chitin degradation to ethanol
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gluconeogenesis I
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L-carnitine degradation III
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L-malate degradation II
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methylgallate degradation
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protocatechuate degradation I (meta-cleavage pathway)
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syringate degradation
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gluconeogenesis
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Pyruvate metabolism
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SYSTEMATIC NAME
IUBMB Comments
(S)-malate:NAD+ oxidoreductase (oxaloacetate-decarboxylating)
Unlike EC 1.1.1.39, malate dehydrogenase (decarboxylating), this enzyme can also decarboxylate oxaloacetate. cf. EC 1.1.1.40, malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+).
CAS REGISTRY NUMBER
COMMENTARY hide
9080-52-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
Amaranthus cruentus
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
archaebacterium
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
strain IFO13182
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
Eriochloa borumensis
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Manually annotated by BRENDA team
Erwinia aroidea
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
quail
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Automatic Mining of ENzyme DAta
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1016405 A+, 1106056 A+, 1144829 A+, 1200284 A+, 1232039 A+, 1249447 A+, 1332370 A+, 1332507 A+, 1332565 A+, 1332621 A+, 1332729 A+, 1332880 A+, 1336547 A+, 1336889 A+, 1336929 A+, 1370382 A+, 1375426 A+, 1375546 A+, 1427127 A+, 1427134 A+, 1427148 A+, 1427149 A+, 1427159 A+, 1427177 A+, 1427180 A+, 1427187 A+, 1427190 A+, 1427193 A+, 1427209 A+, 1427223 A+, 1427225 A+, 1427229 A+, 1427239 A+, 1427252 A+, 1427254 A+, 1427257 A+, 1427265 A+, 1427273 A+, 1427294 A+, 1427327 A+, 1427367 A+, 1427377 A+, 1427386 A+, 1427488 A+, 1427493 A+, 1427509 A+, 1433468 A+, 1433637 A+, 1433639 A+, 1434482 A+, 1457049 A+, 1470471 A+, 1573329 A+, 1573372 A+, 1573628 A+, 1574030 A+, 1598386 A+, 1651433 A+, 1655838 A+, 1683178 A+, 1683184 A+, 1684939 A+, 1686148 A+, 1693766 A+, 1696525 A+, 1697164 A+, 1697266 A+, 1697275 A+, 1698509 A+, 1698606 A+, 1698927 A+, 1699174 A+, 1699176 A+, 1699178 A+, 1699180 A+, 1699182 A+, 1699186 A+, 1699187 A+, 1699191 A+, 1699196 A+, 1699199 A+, 1699200 A+, 1699214 A+, 1699216 A+, 1699218 A+, 1699226 A+, 1699237 A+, 1699240 A+, 1699251 A+, 1699252 A+, 1699253 A+, 1699255 A+, 1699257 A+, 1699263 A+, 1699267 A+, 1699269 A+, 1699273 A+, 1699282 A+, 1699287 A+, 1699288 A+, 1699294 A+, 1699299 A+, 1699307 A+, 1699312 A+, 1699321 A+, 1699323 A+, 1699325 A+, 1699328 A+, 1699329 A+, 1699330 A+, 1699352 A+, 1699359 A+, 1699363 A+, 1699365 A+, 1699367 A+, 1699371 A+, 1699375 A+, 1699377 A+, 1699380 A+, 1699382 A+, 1699384 A+, 1699386 A+, 1699393 A+, 1699394 A+, 1699395 A+, 1699396 A+, 1699399 A+, 1699400 A+, 1699408 A+, 1699410 A+, 1699417 A+, 1699428 A+, 1699434 A+, 1699450 A+, 1699458 A+, 1699468 A+, 1699481 A+, 1699484 A+, 1699502 A+, 1699511 A+, 1699534 A+, 1699538 A+, 1699611 A+, 2167312 A+, 2241782 A+, 2241783 A+, 2241785 A+, 2241786 A+, 2241788 A+, 2241789 A+, 2241791 A+, 2241793 A+, 2241794 A+, 2241795 A+, 2241796 A+, 2241798 A+, 2241802 A+, 2241808 A+, 2241810 A+, 2241818 A+, 2241819 A+, 2241822 A+, 2241825 A+, 2241827 A+, 2241828 A+, 2241829 A+, 2241830 A+, 2241841 A+, 2241864 A+, 2242603 A+, 2350670 A+, 2468738 A+, 2470347 A+, 2504064 A+, 2510290 A+, 2510297 A+, 2552281 A+, 2552282 A+, 2552311 A+, 2552346 A+, 2557331 A+, 2690899 A+, 2750842 A+, 2750920 A+, 2751741 A+, 2752332 A+, 2762300 A+, 2762522 A+, 2762523 A+, 2762524 A+, 2762525 A+, 2762527 A+, 2762529 A+, 2762532 A+, 2762535 A+, 2762536 A+, 2762543 A+, 2762547 A+, 2762548 A+, 2762553 A+, 2762559 A+, 2762561 A+, 2762562 A+, 2762563 A+, 2762572 A+, 2762573 A+, 2762575 A+, 2762576 A+, 2762579 A+, 2762582 A+, 2762603 A+, 2762614 A+, 2762618 A+, 2762630 A+, 2762635 A+, 667894 A+, 686125 A+, 687568 A+, 782425 A+, 782679 A+, 802142 A+, 892090 A+, 998091 A+, 998102 A+, 998105 A+, 998106 A+, 998113 A+, 998123 A+, 998124 A+, 998138 A+, 998139 A+, 998155 A+, 998168 A+, 998169 A+, 998180 A+, 998181 A+, 998189 A+, 998191 A+, 998192 A+, 998215 A+, 998219 A+, 998220 A+, 998227 A+, 998230 A+, 998233 A+, 998234 A+, 998236 A+, 998237 A+, 998238 A+, 998241 A+, 998252 A+, 998261 A+, 998263 A+, 998266 A+, 998271 A+, 998272 A+, 998277 A+, 998278 A+, 998288 A+, 998309 A+, 998321 A+, 998384 A+, 998385 A+, 998423 A+, 999880 A+, 1336628 A++, 1427123 A++, 1699219 A++, 998111 A++, 779556 A+++, 804423 A+++, 997081 A+++, 997588 A+++, 998082 A+++, 998088 A+++, 998090 A+++, 998099 A+++, 998112 A+++, 998115 A+++, 998117 A+++, 998122 A+++, 998126 A+++, 998127 A+++, 998129 A+++, 998131 A+++, 998133 A+++, 998136 A+++, 998137 A+++, 998140 A+++, 998141 A+++, 998146 A+++, 998149 A+++, 998157 A+++, 998158 A+++, 998159 A+++, 998161 A+++, 998164 A+++, 998165 A+++, 998167 A+++, 998170 A+++, 998171 A+++, 998172 A+++, 998175 A+++, 998176 A+++, 998232 A+++, 998235 A+++, 998239 A+++, 998240 A+++, 998244 A+++, 998251 A+++, 998260 A+++, 998262 A+++, 998280 A+++, 998281 A+++, 998284 A+++, 998292 A+++, 998301 A+++, 998308 A+++, 998312 A+++, 998319 A+++, 998326 A+++, 998332 A+++, 998336 A+++, 998337 A+++, 998338 A+++, 998347 A+++, 998361 A+++
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
toad
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
trout
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
yeasts
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Automatic Mining of ENzyme DAta
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-malate + NAD+
CO2 + pyruvate + NADH
show the reaction diagram
(S)-malate + NAD+
pyruvate + CO2 + NADH
show the reaction diagram
(S)-malate + NADP+
CO2 + pyruvate + NADPH
show the reaction diagram
Oxaloacetate
CO2 + pyruvate
show the reaction diagram
oxaloacetate + NADH
(S)-malate + NAD+
show the reaction diagram
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divalent metal ions are required
-
?
oxaloacetate + NADH
L-malate + NAD+
show the reaction diagram
-
-
-
?
pyruvate + NADH + H+
(S)-lactate + NAD+
show the reaction diagram
pyruvate + NADH + H+
L-lactate + NAD+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-malate + NAD+
CO2 + pyruvate + NADH
show the reaction diagram
(S)-malate + NAD+
pyruvate + CO2 + NADH
show the reaction diagram
(S)-malate + NADP+
CO2 + pyruvate + NADPH
show the reaction diagram
-
20% activity compared to cofactor NAD+
-
?
Oxaloacetate
CO2 + pyruvate
show the reaction diagram
oxaloacetate + NADH
L-malate + NAD+
show the reaction diagram
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?
pyruvate + NADH + H+
L-lactate + NAD+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cs+
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30 mM Na+L-malate instead of K+L-malate reduces enzyme activity to 18% in the presence of 3 mM MnCl2, activity is restored with 5 or 50 mM CsCl
K+
-
30 mM Na+L-malate instead of K+L-malate reduces enzyme activity to 18% in the presence of 3 mM MnCl2, activity is restored with 50 mM KCl
Ni2+
-
requirement for divalent cation
Zn2+
-
requirement for divalent cation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
0.025 mM, 70% activity lost after 5 min
1-(5-O-phosphono-beta-D-ribofuranosyl)-4-[2-(trifluoromethyl)phenyl]-1H-1,2,3-triazole
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2-methylbutanoate
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5.22 mM, competitive inhibitor, 60% inhibition at pH 7.5, 54% inhibition at pH 7.0
2-methylpentanoate
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5.22 mM, competitive inhibitor, 45% inhibition at pH 7.5, 40% inhibition at pH 7.0
4,4'-dithiodipyridine
-
sulfhydryl reagent, rapid and complete loss of activity, presence of Mg2+ or Mn2+ enhances inactivation rate
4-(2-aminophenyl)-1-(5-O-phosphono-beta-D-ribofuranosyl)-1H-1,2,3-triazole
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4-(2-fluorophenyl)-1-(5-O-phosphono-beta-D-ribofuranosyl)-1H-1,2,3-triazole
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4-(2-hydroxyethyl)-1-(5-O-phosphono-beta-D-ribofuranosyl)-1H-1,2,3-triazole
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4-(2-methylphenyl)-1-(5-O-phosphono-beta-D-ribofuranosyl)-1H-1,2,3-triazole
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4-(4-fluorophenyl)-1-(5-O-phosphono-beta-D-ribofuranosyl)-1H-1,2,3-triazole
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4-(4-methoxyphenyl)-1-(5-O-phosphono-beta-D-ribofuranosyl)-1H-1,2,3-triazole
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4-(naphthalen-1-yl)-1-(5-O-phosphono-beta-D-ribofuranosyl)-1H-1,2,3-triazole
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4-phenyl-1-(5-O-phosphono-beta-D-ribofuranosyl)-1H-1,2,3-triazole
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5,5'-dithiobis(2-nitrobenzoic acid)
acetyl-CoA
AgNO3
-
inhibition with 20 mM, complete inhibition with 300 mM
Cl-
-
competitive vs. malate and NAD+
D-malate
-
competitive inhibition
diethyldicarbonate
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80% inactivation after treatment with 5 mM diethyldicarbonate for 25 min, biphasic inactivation, 40-50% inactivation in first phase, 1-2 histidine residues are acylated by diethyldicarbonate, 250 mM malate provides complete protection, 50% protection with 50 mM MgSO4, 55% enzyme activity is restored with 0.5 M hydroxylamine
iodoacetate
malonate
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competitive inhibition
N-acetyl-1-(5-O-phosphono-beta-D-ribofuranosyl)-1H-1,2,3-triazol-4-amine
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N-benzoyl-1-(5-O-phosphono-beta-D-ribofuranosyl)-1H-1,2,3-triazol-4-amine
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N-ethylmaleimide
oxaloacetate
p-(chloromercuri)benzoate
succinate
Tartronate
-
competitive inhibition vs. malate, noncompetitve vs. NAD+
tiglic acid
-
5.22 mM, competitive inhibitor, 58% inhibition at pH 7.5, 52% inhibition at pH 7.0
Zn2+
-
5 mM ZnCl2
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoic acid)
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0.5 mM, 2.7fold increase in oxaloacetate reduction activity
alpha-methyl-DL-aspartate
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1.35fold activation
aspartate
ATP
-
68% activation, 2 mM ATP, 0.005 mM CoA, 5 mM malate, 2 mM NAD+, 4 mM Mn2+
beta-methyl-DL-aspartate
-
1.34fold activation
D-Aspartate
-
2 mM, 1.27fold activation
fumarate
L-aspartate
N-ethylmaleimide
-
1 mM, 8.9fold increase in oxaloacetate reduction activity
NH4+
-
30 mM Na+L-malate instead of K+L-malate reduces enzyme activity to 18% in the presence of 3 mM MnCl2, activity is restored with 5 or 50 mM NH4Cl
p-chloromercuribenzoate
-
0.02 mM, 2.5fold increase in oxaloacetate reduction activity
phosphoenolpyruvate
-
0.5 mM
succinate
-
-
UTP
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35% activation, 2 mM UTP, 0.005 mM CoA, 5 mM malate, 2 mM NAD+, 4 mM Mn2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.42
(S)-malate
-
pH 7.2, 25°C, recombinant enzyme
0.19 - 50
L-Malate
0.12 - 0.32
Mn2+
0.004 - 0.51
NAD+
0.025
NADH
-
reduction of oxaloacetate
2.1 - 5
oxaloacetate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
134.4
(S)-malate
Escherichia coli K-12
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pH 7.2, 25°C, recombinant enzyme
130
L-Malate
Ascaris suum
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native and recombinant enzyme
28 - 30
oxaloacetate
0.06 - 0.09
pyruvate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.017
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water stress plants at daytime or at nighttime
0.036
-
control plants at daytime
0.07
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pyruvate reduction
0.107
-
control plants at nighttime
0.46
Amaranthus cruentus
-
below, extract from leaves under continuous light conditions
0.58
Amaranthus cruentus
-
below, leaf extract
1.7
-
oxaloacetate reduction
30
Erwinia aroidea
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-
38.5
-
recombinant enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
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oxaloacetate reduction
5
-
pyruvate reduction
7.3 - 7.6
7.3
-
assay at
7.3 - 7.6
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-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.9 - 8
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over 70% of maximal activity within this range
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE