Information on EC 1.1.1.353 - versiconal hemiacetal acetate reductase

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The expected taxonomic range for this enzyme is: Aspergillus parasiticus

EC NUMBER
COMMENTARY hide
1.1.1.353
-
RECOMMENDED NAME
GeneOntology No.
versiconal hemiacetal acetate reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
versicolorone + NADP+ = 1'-hydroxyversicolorone + NADPH + H+
show the reaction diagram
(1)
-
-
-
versiconol + NADP+ = versiconal + NADPH + H+
show the reaction diagram
(3)
-
-
-
versiconol acetate + NADP+ = versiconal hemiacetal acetate + NADPH + H+
show the reaction diagram
(2)
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Aflatoxin biosynthesis
-
-
versicolorin B biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
versiconol-acetate:NADP+ oxidoreductase
Isolated from the mold Aspergillus parasiticus. Involved in a metabolic grid that leads to aflatoxin biosynthesis.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1'-hydroxyversicolorone + NADPH + H+
versicolorone + NADP+
show the reaction diagram
versiconal + NADPH + H+
versiconol + NADP+
show the reaction diagram
versiconal hemiacetal acetate + NADPH + H+
versiconol acetate + NADP+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
versiconal + NADPH + H+
versiconol + NADP+
show the reaction diagram
versiconal hemiacetal acetate + NADPH + H+
versiconol acetate + NADP+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0254 - 0.0354
versiconal hemiacetal acetate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00362
-
crude extract, in 60 mM potassium phosphate buffer (pH 7.5), at 37C
0.027
-
isoform VHA reductase II, after 7.5fold purification, in 60 mM potassium phosphate buffer (pH 7.5), at 37C
0.052
-
isoform VHA reductase I, after 14.4fold purification, in 60 mM potassium phosphate buffer (pH 7.5), at 37C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
-
isoform VHA reductase I
9
-
isoform VHA reductase II
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 9
-
no activity is detected below pH 4.0
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
isoform VHA reductase II, isoelectric focusing
6.6
-
isoform VHA reductase I, isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38566
x * 38566, calculated from amino acid sequence
39000
-
10 * 39000, isoform VHA reductase I, SDS-PAGE
40000
-
10 * 40000, isoform VHA reductase II, SDS-PAGE
390000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 38566, calculated from amino acid sequence
homodecamer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate fractionation, phenyl Sepharose column chromatography, DEAE-Sepharose column chromatography, Sephacryl S-300 gel filtration, hydroxylapatite column chromatography, and Matrex gel Green A chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE