The enzyme acts in the opposite direction. In Ocimum basilicum (sweet basil), Clarkia breweri and Petunia hybrida only isoeugenol is formed [1,2]. However in Pimpinella anisum (anise) only anol is formed in vivo, although the cloned enzyme does produce isoeugenol .
the phenylpropene-forming isoeugenol, IGS, synthase belongs to a structural family of NADPH-dependent reductases that also includes eugenol synthase, EGS, pinoresinol–lariciresinol reductase, isoflavone reductase, and phenylcoumaran benzylic ether reductase, evolution and function of EGS1 and IGS1, overview
the IGS gene of Petunia axillaris subsp. parodii contains a frame-shift mutation that renders it inactive. In the absence of IGS activity the coniferyl acetate substrate is converted to dihydroconiferyl acetate, instead that eugenol synthesis is increased, overview
plants synthesize the volatile phenylpropene compounds eugenol and isoeugenol to serve in defense against herbivores and pathogens and to attract pollinators. Clarkia breweri flowers emit a mixture of eugenol and isoeugenol. Eugenol and isoeugenol differ in the position of the double bond in the propene side chain
plants synthesize the volatile phenylpropene compounds eugenol and isoeugenol to serve in defense against herbivores and pathogens and to attract pollinators. Petunia hybrida flowers emit mostly isoeugenol with small amounts of eugenol. Eugenol and isoeugenol differ in the position of the double bond in the propene side chain
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native enzyme 9.2fold from petals by alternating steps of anion exchange and hydrophobic interaction chromatography, co-purification with eugenol synthase, EC 22.214.171.1248. Recombinant His-tagged CbIGS from Escherichia coli by nickel affinity chromatography
recombinant Petunia hybrida IGS expression alone does not increase the concentration of anol or isoeugenol in agroinfiltrated strawberry fruits, concurrent silencing of CHS substantially enhances the yield of the two metabolites
Vassao, D.; Kim, S.; Milhollan, J.; Eichinger, D.; Davin, L.; Lewis, N.
A pinoresinol-lariciresinol reductase homologue from the creosote bush (Larrea tridentata) catalyzes the efficient in vitro conversion of p-coumaryl/coniferyl alcohol esters into the allylphenols chavicol/eugenol, but not the propenylphenols p-anol/isoeug