Information on EC 1.1.1.29 - glycerate dehydrogenase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.1.29
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RECOMMENDED NAME
GeneOntology No.
glycerate dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-glycerate + NAD+ = hydroxypyruvate + NADH + H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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Biosynthesis of secondary metabolites
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Glycine, serine and threonine metabolism
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Glyoxylate and dicarboxylate metabolism
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Metabolic pathways
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Methane metabolism
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Microbial metabolism in diverse environments
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photorespiration
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serine metabolism
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SYSTEMATIC NAME
IUBMB Comments
D-glycerate:NAD+ oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
9028-37-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
kohlrabi
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Manually annotated by BRENDA team
carrot
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
barley
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-
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
parsley
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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enzyme knockdown leads to an inhibition of ascorbic acid uptake
physiological function
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rates of photosynthesis under photorespiratory conditions are slightly reduced in mutant plants lacking isoform hpr1 expression or additionally lacking both of the peroxisomal malate dehydrogenase genes, mutant pmdh1pmdh2hpr1. The CO2 compensation points increase under photorespiratory conditions in both mutants. The increase in CO2 compensation points in the hpr1 and pmdh1pmdh2hpr1 plants is not associated with changes in mitochondrial respiration but with an increase in the non-respiratory CO2 released per Rubisco oxygenation reaction
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glyoxylate + NADH
glycolate + NAD+
show the reaction diagram
hydroxypyruvate + NADH
D-glycerate + NAD+
show the reaction diagram
hydroxypyruvate + NADH + H+
D-glycerate + NAD+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
hydroxypyruvate + NADH
D-glycerate + NAD+
show the reaction diagram
hydroxypyruvate + NADH + H+
D-glycerate + NAD+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Dihydroxyfumarate
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GSH
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about 72% residual activity at 2 mM
Hydroxypyruvate
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product inhibition at a concentration above 4 mM, 99% inhibition of the reverse reaction at 0.5 mM
NADH
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concentration above 0.5 mM
NADPH
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concentration above 0.5 mM
p-chloromercuribenzoate
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phenylhydrazine
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S-nitrosoglutathione
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about 80% residual activity at 2 mM
SIN-1
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about 35% residual activity at 1 mM
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.5 - 20
glyoxylate
0.062 - 0.175
Hydroxypyruvate
0.0058 - 0.055
NADH
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0051
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recombinant enzyme, at pH and °C
additional information
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activities of several Hypomicrobium and Methylobacterium strains listed
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 6.5
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reduction of hydroxypyruvate
9 - 11
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glycerate oxidation
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37000
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2 * 37000, SDS-PAGE
38000
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2 * 38000, SDS-PAGE
45200
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2 * 45200, SDS-PAGE
69000 - 70000
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gel filtration
90000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9
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286506
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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30 min, 13.3% loss of activity
35
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30 min, 30.8% loss of activity
40
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30 min, 100% loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 10 mM potassium phosphate buffer, pH 7.0, 45% v/v glycerol, 1.0 M DTT, 18 months
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4°C, 50 mM Tris-HCl buffer, pH 7.5, containing 10% glycerol and 1 mM dithiothreitol, 2 months, at least 90% of inital activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
glutathione-Sepharose 4B bead column chromatography, and Superdex 200 gel filtration
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LYTRA column chromatography, and Superdex 200 gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain BIVU0811
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme expression is decreased or lost in hepatocellular carcinoma
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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glyoxylate reductase/hydroxypyruvate reductase is a prognostic marker for hepatocellular carcinoma patients after curative resection
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