Information on EC 1.1.1.281 - GDP-4-dehydro-6-deoxy-D-mannose reductase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.281
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RECOMMENDED NAME
GeneOntology No.
GDP-4-dehydro-6-deoxy-D-mannose reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GDP-alpha-D-rhamnose + NAD(P)+ = GDP-4-dehydro-alpha-D-rhamnose + NAD(P)H + H+
show the reaction diagram
; This enzyme differs from EC 1.1.1.187, GDP-4-dehydro-D-rhamnose reductase, in that the only product formed is GDP-D-rhamnose (GDP-6-deoxy-D-mannose). D-Rhamnose is a constituent of lipopolysaccharides of Gram-negative plant and human pathogenic bacteria
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
reduction
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Amino sugar and nucleotide sugar metabolism
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Fructose and mannose metabolism
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GDP-D-rhamnose biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
GDP-6-deoxy-alpha-D-mannose:NAD(P)+ 4-oxidoreductase (D-rhamnose-forming)
This enzyme differs from EC 1.1.1.187, GDP-4-dehydro-D-rhamnose reductase, in that the only product formed is GDP-alpha-D-rhamnose. D-Rhamnose is a constituent of lipopolysaccharides of Gram-negative plant and human pathogenic bacteria.
CAS REGISTRY NUMBER
COMMENTARY hide
9075-56-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
; expression in Saccharomyces cerevisiae
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GDP-4-dehydro-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-D-mannose + NADP+
show the reaction diagram
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?
GDP-6-deoxy-D-lyxo-4-hexulose + NADH + H+
GDP-alpha-D-rhamnose + NAD+
show the reaction diagram
GDP-6-deoxy-D-lyxo-4-hexulose + NADPH + H+
GDP-alpha-D-rhamnose + NADP+
show the reaction diagram
GDP-6-deoxy-D-mannose + NAD(P)+
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDP-4-dehydro-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-D-mannose + NADP+
show the reaction diagram
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-
?
GDP-6-deoxy-D-mannose + NAD(P)+
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
show the reaction diagram
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33900
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x * 34000, SDS-PAGE of FLAG-tagged recombinant enzyme, x * 33900, calculated
34000
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x * 34000, recombinant FLAG-tagged enzyme; x * 34000, SDS-PAGE of FLAG-tagged recombinant enzyme, x * 33900, calculated
38100
x * 38100, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Saccharomyces cerevisiae
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overexpression in Escherichia coli
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
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generating GDP-rhamnose for in vitro rhamnosylation of glycoproteins and glycopeptides; generation of GDP-rhamnose by coexpression of enzyme with GDP-mannose-4,6-dehydratase in Saccharomyces cerevisiae; the functional expression of the Pseudomonas aeruginosa enzyme in Saccharomyces cerevisiae will provide a tool for generating GDP-rhamnose for in vitro rhamnosylation of glycoprotein and glycopeptides