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Information on EC 1.1.1.27 - L-lactate dehydrogenase and Organism(s) Rattus norvegicus

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IUBMB Comments
Also oxidizes other (S)-2-hydroxymonocarboxylic acids. NADP+ also acts, more slowly, with the animal, but not the bacterial, enzyme.
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Rattus norvegicus
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The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
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(S)-lactate
+
NAD+
=
pyruvate
+
NADH
+
H+
+
=
+
+
Synonyms
lactate dehydrogenase, lactic dehydrogenase, ldh-a, lactate dehydrogenase a, l-lactate dehydrogenase, ldh-5, lactic acid dehydrogenase, ldh-1, pfldh, alpha-hydroxybutyrate dehydrogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dehydrogenase, lactate
-
-
-
-
Epsilon crystallin
-
-
-
-
epsilon-crystallin
-
-
-
-
Immunogenic protein p36
-
-
-
-
L(+)-nLDH
-
-
-
-
L-(+)-lactate dehydrogenase
-
-
-
-
L-lactic acid dehydrogenase
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-
-
-
L-lactic dehydrogenase
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-
-
-
L-LDH
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-
-
-
lactate dehydrogenase
lactate dehydrogenase NAD-dependent
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-
-
-
lactic acid dehydrogenase
-
-
-
-
lactic dehydrogenase
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-
-
-
NAD-lactate dehydrogenase
-
-
-
-
proteins, specific or class, anoxic stress response, p34
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
-
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redox reaction
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-
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reduction
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-
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-
SYSTEMATIC NAME
IUBMB Comments
(S)-lactate:NAD+ oxidoreductase
Also oxidizes other (S)-2-hydroxymonocarboxylic acids. NADP+ also acts, more slowly, with the animal, but not the bacterial, enzyme.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-60-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-lactate + NAD+
pyruvate + NADH + H+
show the reaction diagram
-
-
-
-
?
pyruvate + NADH + H+
L-lactate + NAD+
show the reaction diagram
-
-
-
-
?
pyruvate + NADH + H+
lactate + NAD+
show the reaction diagram
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-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-nitropropionate
-
-
dicholesteroyl diselenide
-
inhibition of different isoforms of lactate dehydrogenase by dicholesteroyl diselenide possibly involves the modification of the thiol groups at the NAD+ binding site of the enzyme. Exerts profound concentration dependent inhibitory effect on the activity of renal LDH. Inhibitory effect on hepatic LDH is markedly pronounced at 2, 4, 8 and 10 microM. Strongly inhibits cardiac LDH activity when NAD+ is omitted from the pre-incubating medium than when lactate is absent from the pre-incubating medium, significantly inhibits the enzyme activity at 1, 2, 4, and 8 microM
diphenyl diselenide
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inhibition of different isoforms of lactate dehydrogenase by diphenyl diselenide possibly involves the modification of the thiol groups at the NAD+ binding site of the enzyme. Inhibitory effect on hepatic LDH is markedly different at 10 microM. Markedly inhibits cardiac LDH activity at 8 and 10 microM
methylmalonate
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IC50: 4.6 mM (enzyme from brain), 4.6 mM (enzyme from liver)
additional information
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short-term storage in Krebs-Henseleit buffer for 24 h at 4°C does not affect LDH activity, but a 42% decline occurs at 23°C. After 48 h, activity declines 11% at 4°C and 98% at 23°C. Frozen storage results in a 40% loss at -80°C and a 79% loss at -20°C
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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hearts perfused with Krebs-Henseleit buffer, subjected to 30 min of global ischemia followed by normoxic reperfusion, which causes tissue damage and elevate LDH release
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.79
(S)-lactate
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-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.02
methylmalonate
-
enzyme from brain
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.6
methylmalonate
Rattus norvegicus
-
IC50: 4.6 mM (enzyme from brain), 4.6 mM (enzyme from liver)
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
LDHA_RAT
332
0
36451
Swiss-Prot
other Location (Reliability: 2)
LDHB_RAT
334
0
36612
Swiss-Prot
other Location (Reliability: 2)
LDHC_RAT
332
0
35687
Swiss-Prot
other Location (Reliability: 2)
Q7TNA8_RAT
382
0
42049
TrEMBL
Mitochondrion (Reliability: 3)
Q6AYX2_RAT
332
0
35713
TrEMBL
other Location (Reliability: 2)
A0A8I6AAB9_RAT
390
0
42941
TrEMBL
Mitochondrion (Reliability: 1)
A0A8I5ZX39_RAT
361
0
39747
TrEMBL
other Location (Reliability: 3)
A0A8I6ASS6_RAT
331
0
36480
TrEMBL
other Location (Reliability: 1)
A0A8I6AMT0_RAT
362
0
39603
TrEMBL
other Location (Reliability: 3)
H9N9H4_RAT
274
0
29554
TrEMBL
other Location (Reliability: 2)
Q6P9U7_RAT
270
0
29832
TrEMBL
other Location (Reliability: 1)
B5DEN4_RAT
332
0
36451
TrEMBL
other Location (Reliability: 2)
A0A8I6AT42_RAT
308
0
34082
TrEMBL
other Location (Reliability: 3)
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, Krebs-Henseleit buffer, 24 h
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partially purified renal LDH
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
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reproducible and validated LDH assay optimized for several cell types for application in clinical medicine and biomedical sciences. Assay is cost effective and allows for experiment-specific optimization
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Saad, L.O.; Mirandola, S.R.; Maciel, E.N.; Castilho, R.F.
Lactate dehydrogenase activity is inhibited by methylmalonate in vitro
Neurochem. Res.
31
541-548
2006
Bos taurus, Rattus norvegicus
Manually annotated by BRENDA team
Kade, I.J.; Paixao, M.W.; Rodrigues, O.E.; Ibukun, E.O.; Braga, A.L.; Zeni, G.; Nogueira, C.W.; Rocha, J.B.
Studies on the antioxidant effect and interaction of diphenyl diselenide and dicholesteroyl diselenide with hepatic delta-aminolevulinic acid dehydratase and isoforms of lactate dehydrogenase
Toxicol. In Vitro
23
14-20
2009
Rattus norvegicus
Manually annotated by BRENDA team
Starnes, J.
Effect of storage conditions on lactate dehydrogenase released from perfused hearts
Int. J. Cardiol.
127
114-116
2008
Rattus norvegicus
Manually annotated by BRENDA team
Kaja, S.; Payne, A.J.; Naumchuk, Y.; Koulen, P.
Quantification of lactate dehydrogenase for cell viability testing using cell lines and primary cultured astrocytes
Curr. Protoc. Toxicol.
72
2.26.1-2.26.10
2017
Rattus norvegicus
Manually annotated by BRENDA team