Information on EC 1.1.1.227 - (-)-borneol dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.227
-
RECOMMENDED NAME
GeneOntology No.
(-)-borneol dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(-)-borneol + NAD+ = (-)-camphor + NADH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
(-)-camphor biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
(-)-borneol:NAD+ oxidoreductase
NADP+ can also act, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
111940-48-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene LiBDH
-
-
Manually annotated by BRENDA team
tansy
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
-
the enzyme is a member of the SDR superfamily of enzymes
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(-)-borneol + NAD+
(-)-camphor + NADH
show the reaction diagram
(-)-borneol + NAD+
(-)-camphor + NADH + H+
show the reaction diagram
-
-
-
-
ir
additional information
?
-
-
no activity with other monoterpenes, e.g. alpha terpineol, 1,8-cineole, citronellol, linalool, lavandulol, nerol, geraniol, and perillyl alcohol, and sesquiterpene farnesol, or with menthol, overview
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-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(-)-borneol + NAD+
(-)-camphor + NADH
show the reaction diagram
-
biosynthesis of camphor from borneol
-
?
(-)-borneol + NAD+
(-)-camphor + NADH + H+
show the reaction diagram
-
-
-
-
ir
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
-
preferred cofactor
additional information
-
no activity with NADP+
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.053
(-)-borneol
-
pH 8.0, 30C, recombinant enzyme
additional information
additional information
-
Michaelis-Menten kinetics, overview
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0004
(-)-borneol
Lavandula x intermedia
-
pH 8.0, 30C, recombinant enzyme
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0075
(-)-borneol
Lavandula x intermedia
-
pH 8.0, 30C, recombinant enzyme
8975
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
specifically in glandular trichomes of mature flowers
Manually annotated by BRENDA team
additional information
-
the gene exppression is regulated in a tissue-specific manner
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27500
-
x * 27500, about, sequence calculation
70000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 27500, about, sequence calculation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
stable to lyophilization
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-40C, lyophilized, under argon, stable for several months without deterioration
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3)pLysSby nickel affinity chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene LiBDH, DNA and amino acid sequence determination and analysis, phylogenetic tree, gene expression is regulated in a tissue-specific manner, expression of the His-tagged enzyme in Escherichia coli strain Rosetta (DE3)pLysS
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
-
the gene encoding the borneol dhdrogenase is a target for metabolic engineering for improvement of essential oil production