Information on EC 1.1.1.197 - 15-hydroxyprostaglandin dehydrogenase (NADP+)

Word Map on EC 1.1.1.197
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.197
-
RECOMMENDED NAME
GeneOntology No.
15-hydroxyprostaglandin dehydrogenase (NADP+)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-13-enoate + NADP+ = (13E)-11alpha-hydroxy-9,15-dioxoprost-13-enoate + NADPH + H+
show the reaction diagram
Acts on prostaglandins E2, F2alpha and B1, but not on prostaglandin D2[cf. EC1.1.1.141 (NADP)]. May be identical with EC1.1.1.189 prostaglandin-E2 9-reductase
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-13-enoate:NADP+ 15-oxidoreductase
Acts on prostaglandins E2, F2alpha and B1, but not on prostaglandin D2 [cf. EC 1.1.1.141 15-hydroxyprostaglandin dehydrogenase (NAD+) and EC 1.1.1.196 15-hydroxyprostaglandin-D dehydrogenase (NADP+)]. May be identical with EC 1.1.1.189 prostaglandin-E2 9-reductase.
CAS REGISTRY NUMBER
COMMENTARY hide
54989-39-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
bovine
-
-
Manually annotated by BRENDA team
-
Swissprot
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
rat, Holtzman
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-methyl-3-glutathionyl-1,4-benzoquinone + NADPH
? + NADP+
show the reaction diagram
-
toluquinone thioether
-
-
?
2-methyl-3-glutathionyl-1,4-naphthoquinone + NADPH
? + NADP+
show the reaction diagram
-
menadione thioether
-
-
?
4,5-benzo[a]pyrenequinone + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
5,6-chrysenequinone + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
7,8-benzo[a]pyrenequinone + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
9,10-phenanthrenequinone + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
acid form of latanoprost + NADP+
? + NADPH
show the reaction diagram
-
-
-
?
glutathione-9-hydroxy-prostaglandin A1 thioether + NADP+
? + NADPH
show the reaction diagram
-
-
-
-
?
glutathione-prostaglandin A1 thioether + NADP+
? + NADPH
show the reaction diagram
-
PGA1-SG, excellent substrate for the placental enzyme
-
-
?
latanoprost + NADP+
? + NADPH
show the reaction diagram
-
-
-
?
menadione + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
prostaglandin A1 + NADP+
15-oxoprostaglandin A1 + NADPH + H+
show the reaction diagram
-
-
-
-
?
prostaglandin B1 + NADP+
15-oxoprostaglandin B1 + NADPH + H+
show the reaction diagram
-
-
-
-
?
prostaglandin E + NADP+
? + NADPH
show the reaction diagram
prostaglandin E1 + NADP+
15-keto-prostaglandin E1 + NADPH + H+
show the reaction diagram
-
-
-
-
?
prostaglandin E2 + NADP+
15-oxoprostaglandin E2 + NADPH + H+
show the reaction diagram
-
-
-
-
?
prostaglandin E2 + NADP+
prostaglandin F2alpha + NADPH
show the reaction diagram
prostaglandin F + NADP+
? + NADPH
show the reaction diagram
prostaglandin F2alpha + NADP+
prostaglandin E1 + NADPH
show the reaction diagram
prostaglandin I2 + NADP+
? + NADPH
show the reaction diagram
-
poor substrate for the enzyme
-
-
?
thromboxane B2 + NADP+
? + NADPH
show the reaction diagram
-
slowly oxidized
-
-
?
toluquinone + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
the trans-dihydrodiols trans-dihydroxy-4,5-dihydrobenzo[alpha]pyrene, trans-7,8-dihydroxy-7,8-dihydrobenzo[alpha]pyrene, trans-3,4-dihydroxy-3,4-dihydrobenz[alpha]anthracene and its 7-methyl, 12-methyl, and 7,12-dimethyl derivatives are not substrates for the enzyme
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
prostaglandin E2 + NADP+
15-oxoprostaglandin E2 + NADPH + H+
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
NADPH
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-glutathionyl-2-hydroxy-3(allyloxy)propane
-
competitive inhibition of prostaglandin B1 oxidation
beta-mercaptoacetate
-
-
beta-mercaptolactate
-
-
beta-Mercaptopyruvate
-
-
glutathione-9-hydroxy-prostaglandin A1 thioether
-
inhibition of polycyclic aromatic hydrocarbon quinone reduction
-
GSSG
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0024
2-methyl-3-glutathionyl-1,4-benzoquinone
-
-
0.0034
2-methyl-3-glutathionyl-1,4-naphthoquinone
-
-
0.00018
4,5-benzo[a]pyrenequinone
-
-
0.00051
5,6-chrysenequinone
-
-
0.00087
7,8-benzo[a]pyrenequinone
-
-
-
0.00036
9,10-phenanthrenequinone
-
-
0.00033
glutathione-prostaglandin A1 thioether
-
-
-
0.0076
menadione
-
-
0.09
prostaglandin A1
-
-
0.157
prostaglandin E1
-
-
0.03
toluquinone
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0012
substrate latanoprost, pH 7.0, 25C
0.0022
substrate acid form of latanoprost, pH 7.0, 25C
0.0061
substrate prostaglandin F2alpha, cosubstrate NAD+, pH 7.0, 25C
0.0098
substrate prostaglandin E2, cosubstrate NADP+, pH 7.0, 25C
0.0243
substrate prostaglandin F2alpha, cosubstrate NADP+, pH 7.0, 25C
0.09
-
-
6.8
-
form I, pI 5.8
7.5
-
form II, pI 4.8
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
mesenteric vessels, arteries and veins
Manually annotated by BRENDA team
epithelial cells of cornea
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30500
x * 30500, deduced from gene sequence, x * 31000, SDS-PAGE
31000
x * 30500, deduced from gene sequence, x * 31000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 30500, deduced from gene sequence, x * 31000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE