Information on EC 1.1.1.187 - GDP-4-dehydro-D-rhamnose reductase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.1.1.187
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RECOMMENDED NAME
GeneOntology No.
GDP-4-dehydro-D-rhamnose reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GDP-alpha-D-rhamnose + NAD(P)+ = GDP-4-dehydro-alpha-D-rhamnose + NAD(P)H + H+
show the reaction diagram
In the reverse reaction, a mixture of GDP-D-rhamnose and its C-4 epimer is formed
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
-
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redox reaction
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-
-
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reduction
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Amino sugar and nucleotide sugar metabolism
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Fructose and mannose metabolism
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GDP-6-deoxy-D-talose biosynthesis
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GDP-D-rhamnose biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
GDP-6-deoxy-alpha-D-mannose:NAD(P)+ 4-oxidoreductase
The enzyme, which operates in the opposite direction to that shown, forms a mixture of GDP-alpha-D-rhamnose and its C-4 epimer, GDP-6-deoxy-alpha-D-talose. cf. EC 1.1.1.281, GDP-4-dehydro-6-deoxy-D-mannose reductase and EC 1.1.1.135, GDP-6-deoxy-D-talose 4-dehydrogenase.
CAS REGISTRY NUMBER
COMMENTARY hide
9075-56-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
soil bacterium GS, ATCC 19241
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
GDP-6-deoxy-D-mannose + NAD(P)+
show the reaction diagram
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
GDP-D-talometylose + NAD(P)+
show the reaction diagram
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lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function
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?
GDP-4-dehydro-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-D-talose + NADP+
show the reaction diagram
GDP-6-deoxy-D-lyxo-4-hexulose + NAD(P)H
GDP-alpha-D-rhamnose + NAD(P)+
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
GDP-6-deoxy-D-mannose + NAD(P)+
show the reaction diagram
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
GDP-D-talometylose + NAD(P)+
show the reaction diagram
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lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
GDP-alpha-D-mannose
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competitive inhibitor
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.025 - 0.05
GDP-4-dehydro-6-deoxy-D-mannose
0.027
NADH
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0.028
NADPH
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.72
GDP-mannose
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competitive inhibition
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7
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with NADPH as reductant
8
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with NADH as reductant
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000 - 70000
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gel filtration
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56
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loss of activity in 5 min
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
DTT reduces instability at room temperature from 51% to 35%
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389366
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, half-life: 5 months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity, fractionation-, chromatography-techniques
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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