Information on EC 1.1.1.122 - D-threo-aldose 1-dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
1.1.1.122
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RECOMMENDED NAME
GeneOntology No.
D-threo-aldose 1-dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a D-threo-aldose + NAD+ = a D-threo-aldono-1,5-lactone + NADH + H+
show the reaction diagram
Acts on L-fucose, D-arabinose and L-xylose. The animal enzyme was also shown to act on L-arabinose, and the enzyme from Pseudomonas caryophylli on L-glucose
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Ascorbate and aldarate metabolism
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Biosynthesis of secondary metabolites
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Fructose and mannose metabolism
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Metabolic pathways
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
D-threo-aldose:NAD+ 1-oxidoreductase
Acts on L-fucose, D-arabinose and L-xylose; the animal enzyme was also shown to act on L-arabinose, and the enzyme from Pseudomonas caryophylli on L-glucose.
CAS REGISTRY NUMBER
COMMENTARY hide
9082-70-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain K-28
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Manually annotated by BRENDA team
rabbit
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Manually annotated by BRENDA team
Ovis aries aries
sheep
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Manually annotated by BRENDA team
No. 1143
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Manually annotated by BRENDA team
porcine
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-deoxy-D-ribose + NAD+
? + NADH
show the reaction diagram
Ovis aries aries
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10% of the activity compared to L-fucose or D-arabinose
-
?
3-amino-3-deoxy-D-arabinose + NAD+
3-amino-3-deoxy-D-arabino-1,5-lactone + NADH
show the reaction diagram
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15% of the activity compared to L-fucose
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?
6-deoxy-L-galactose + NAD+
6-deoxy-L-galactono-1,5-lactone + NADH
show the reaction diagram
D-arabinose + NAD+
D-arabinono-1,4-lactone + NADH
show the reaction diagram
D-lyxose + NAD+
? + NADH
show the reaction diagram
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4% of the activity compared to L-fucose
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?
D-ribose + NAD+
? + NADH
show the reaction diagram
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8% of the activity compared to D-arabinose or L-xylose
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?
D-xylose + NAD+
D-xylono-1,5-lactone + NADH + H+
show the reaction diagram
fuconolactone + NADPH + H+
L-fucose + NADP+
show the reaction diagram
L-galactose + NAD+
L-galactono-1,5-lactone + NADH
show the reaction diagram
L-glucose + NAD+
L-glucono-1,5-lactone + NADH
show the reaction diagram
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68% of the activity compared to D-arabinose or L-xylose
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?
L-xylose + NAD+
L-xylono-1,5-lactone + NADH
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-deoxy-D-ribose + NAD+
? + NADH
show the reaction diagram
Ovis aries aries
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10% of the activity compared to L-fucose or D-arabinose
-
?
3-amino-3-deoxy-D-arabinose + NAD+
3-amino-3-deoxy-D-arabino-1,5-lactone + NADH
show the reaction diagram
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15% of the activity compared to L-fucose
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?
6-deoxy-L-galactose + NAD+
6-deoxy-L-galactono-1,5-lactone + NADH
show the reaction diagram
D-arabinose + NAD+
D-arabinono-1,4-lactone + NADH
show the reaction diagram
D-lyxose + NAD+
? + NADH
show the reaction diagram
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4% of the activity compared to L-fucose
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?
D-ribose + NAD+
? + NADH
show the reaction diagram
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8% of the activity compared to D-arabinose or L-xylose
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?
D-xylose + NAD+
D-xylono-1,5-lactone + NADH + H+
show the reaction diagram
L-galactose + NAD+
L-galactono-1,5-lactone + NADH
show the reaction diagram
L-glucose + NAD+
L-glucono-1,5-lactone + NADH
show the reaction diagram
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68% of the activity compared to D-arabinose or L-xylose
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?
L-xylose + NAD+
L-xylono-1,5-lactone + NADH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-Amino-3-deoxy-D-arabinose
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ADP
Ovis aries aries
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slight inhibition
ATP
Ovis aries aries
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slight inhibition
Cd2+
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inhibitory
D-arabinose
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competitive inhibition of L-fucose oxidation at 2.5 mM
D-galactose
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79% inhibition of L-glucose oxidation
D-glucose
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competitive inhibition of L-glucose oxidation, Ki: 130 mM
Heavy metal ions
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strong inhibition
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iodoacetic acid
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10% inhibition at 1 mM
L-arabinose
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74% inhibition of L-glucose oxidation
L-rhamnose
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74% inhibition of L-glucose oxidation
N-ethylmaleimide
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strong inhibition at 0.1 mM
NADH
Ovis aries aries
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Ki: 0.04 mM
p-chloromercuribenzoate
p-hydroxymercuribenzoate
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20% loss of activity, saturated
sodium iodoacetate
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20% loss of activity at 20 mM, pH 8.0
thiol reagents
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strong inhibition
Zn2+
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40% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
hydroxylamine
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stimulation at high concentrations
KNO3
Ovis aries aries
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activation at 1-1.3 M, optimal pH shifts to 9-9.5
NaBr
Ovis aries aries
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activation at 1-1.3 M, optimal pH shifts to 9-9.5
NaCl
Ovis aries aries
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activation at 1-1.3 M, optimal pH shifts to 9-9.5
NaNO3
Ovis aries aries
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activation at 1-1.3 M, optimal pH shifts to 9-9.5
Tris-HCl
Ovis aries aries
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activation at 1-1.3 M, optimal pH shifts to 9-9.5
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8
3-amino-D-arabinose
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0.07 - 12.8
6-deoxy-L-galactose
0.4 - 40
D-arabinose
11.1
D-galactose
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48
D-Lyxose
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0.2 - 50
L-galactose
4.5 - 44
L-Xylose
0.01 - 1.89
NAD+
0.02 - 0.6
NADP+
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.03
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purified enzyme
1.88
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crude enzyme, cells grown in sucrose-containing medium
3.66
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purified enzyme
53
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purified enzyme
375
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purified enzyme
433
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purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.7
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10.4
Ovis aries aries
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ionic strength: 0.05
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9
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active within
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
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1 * 37000 + 1 * 28000, SDS-PAGE
30000
Ovis aries aries
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x * 30000, SDS-PAGE, x * 35000, guanidinium-HCl gel filtration
35000
Ovis aries aries
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x * 30000, SDS-PAGE, x * 35000, guanidinium-HCl gel filtration
37000
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1 * 37000 + 1 * 28000, SDS-PAGE
37291
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4 * 37291, calculated from sequence
40000
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gel filtration
64000
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gel filtration
71000
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gel filtration
82000 - 123000
Ovis aries aries
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sedimentation equilibrium analysis: 123000 Da, gel filtration: 82000 Da
92000
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gel filtration
96000
Ovis aries aries
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electro focusing
100000
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gel filtration
149700
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mass spectrometry
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
Ovis aries aries
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x * 30000, SDS-PAGE, x * 35000, guanidinium-HCl gel filtration
dimer
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1 * 37000 + 1 * 28000, SDS-PAGE
homotetramer
monomer
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1 * 40000, SDS-PAGE
tetramer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
the structure is solved to a resolution of 1.80 A by single-wavelength anomalous diffraction and phased using the two endogenous zinc ions per subunit. The structure reveals a catalytic and cofactor binding domain
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 12
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stable between
285805
7.5 - 9
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stable within
285798
7.5 - 9.5
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stable within
285800
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
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rapid loss of activity
25
Ovis aries aries
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at pH 7.2: half-life: 1.8 days
45
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stable up to
85
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half-life: 42 min
90
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half-life: 26 min
additional information
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thermal stability of the enzyme is increased by means of dextran-modification or addition of chelating reagents at high concentrations
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
crude enzyme preparations stable, purified enzyme very unstable
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NAD+ stabilizes
Ovis aries aries
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sensitive to repeated freezing and thawing
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stable to refrigeration at 5C for a week
Ovis aries aries
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thermal stability of the enzyme is increased by means of dextran-modification or addition of chelating reagents at high concentrations
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 2 months
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-20C, minimum concentration: 1 mg/ml protein, 1 month
Ovis aries aries
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-20C, presence of NAD+, 1 year
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4C, 50% loss of activity within 1 month
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4C, pH 7.2, half-life: 39 days
Ovis aries aries
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity, chromatography steps, preparative gel electrophoresis
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to homogeneity, chromatography steps, preparative gel electrophoresis, affinity chromatography
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to homogeneity, chromatography techniques
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
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determination of bound-fucose in biological materials by a coupled enzymatic method in a single buffer system
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