Information on EC 1.1.1.102 - 3-dehydrosphinganine reductase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.102
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RECOMMENDED NAME
GeneOntology No.
3-dehydrosphinganine reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
sphinganine + NADP+ = 3-dehydrosphinganine + NADPH + H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ceramide de novo biosynthesis
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Metabolic pathways
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sphingolipid biosynthesis (plants)
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sphingolipid biosynthesis (yeast)
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Sphingolipid metabolism
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ceramide biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
D-erythro-dihydrosphingosine:NADP+ 3-oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
37250-36-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
genes At3g06060/TSC10A and At5g19200/TSC10B, i.e. tsc10A and tsc10B
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Manually annotated by BRENDA team
gene ksrA
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Manually annotated by BRENDA team
gene ksr1
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Manually annotated by BRENDA team
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S4SCP5
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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perturbation of sphingolipid biosynthesis in the Arabidopsis tsc10a mutant leads to an altered leaf ionome, including increases in Na, K, and Rb and decreases in Mg, Ca, Fe, and Mo, associated with increases in root suberin and alterations in Fe homeostasis. Loss of function of both TSC10A and TSC10B Is lethal in Arabidopsis thaliana
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-2-amino-1-hydroxyeicosane-3-one + NADPH
(S)-2-amino-1-(R)-3-dihydroxyeicosane + NADP+
show the reaction diagram
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slower reduced than (S)-2-amino-1-hydroxyoctadecane-3-one
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?
(S)-2-amino-1-hydroxyoctadecane-3-one + NADPH
(S)-2-amino-1-(R)-3-dihydroxyoctadecane + NADP+
show the reaction diagram
2-amino-1-hydroxyoctadecane-3-one + NADPH
2-amino-1,3-dihydroxyoctadecane + NADP+
show the reaction diagram
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-
-
?
2-amino-1-hydroxyoctadecane-3-one + NADPH
2-amino-1-(R)-3-dihydroxyoctadecane + NADP+
show the reaction diagram
3-dehydrosphinganine + NADPH
sphinganine + NADP+
show the reaction diagram
3-dehydrosphinganine + NADPH + H+
sphinganine + NADP+
show the reaction diagram
3-ketodihydrosphingosine + NADPH
dihydrosphingosine + NADP+
show the reaction diagram
3-oxosphinganine + NADPH + H+
D-erythro-dihydrosphingosine + L-threo-dihydrosphingosine + NADP+
show the reaction diagram
3-oxosphinganine + NADPH + H+
dihydrosphingosine + NADP+
show the reaction diagram
S4SCP5
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-2-amino-1-hydroxyoctadecane-3-one + NADPH
(S)-2-amino-1-(R)-3-dihydroxyoctadecane + NADP+
show the reaction diagram
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enzyme acts at the cytoplasmic side of endoplasmic reticulum
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?
2-amino-1-hydroxyoctadecane-3-one + NADPH
2-amino-1,3-dihydroxyoctadecane + NADP+
show the reaction diagram
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-
?
3-dehydrosphinganine + NADPH
sphinganine + NADP+
show the reaction diagram
3-oxosphinganine + NADPH + H+
D-erythro-dihydrosphingosine + L-threo-dihydrosphingosine + NADP+
show the reaction diagram
3-oxosphinganine + NADPH + H+
dihydrosphingosine + NADP+
show the reaction diagram
S4SCP5
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
siRNA
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reduces expression of transfected FVT1 mRNA and protein by at least 70%
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
tergitol
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0.2%, 10fold activation of 2-amino-1-hydroxyoctadecane-3-one reduction
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.03
(S)-2-amino-1-hydroxyeicosane-3-one
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0.015
(S)-2-amino-1-hydroxyoctadecane-3-one
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0.009
3-dehydrosphinganine
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pH 7.5, recombinant enzyme
0.003
3-ketodihydrosphingosine
pH 7.5, 37°C
0.028
NADPH
pH 7.5, 37°C
additional information
additional information
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binding kinetics of substrate analogues with shorter chain lengths
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.07
3-dehydrosphinganine
Candida albicans
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pH 7.5, recombinant enzyme
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000121
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vesicles from smooth and rough endoplasmic reticulum
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
low expression
Manually annotated by BRENDA team
S4SCP5
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Manually annotated by BRENDA team
pheripheral blood, low expression
Manually annotated by BRENDA team
highest expression
Manually annotated by BRENDA team
high expression
Manually annotated by BRENDA team
high expression
Manually annotated by BRENDA team
low expression
Manually annotated by BRENDA team
additional information
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the 3-KDS reductase genes are functionally redundant and ubiquitously expressed in Arabidopsis thaliana
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
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the plant isozymes contain an N-terminal membrane-spanning domain, subcellular localization study, overview
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
150000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
additional information
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molecular modeling of the enzyme with bound substrates, a significant portion of the aliphatic chain of 3-dehydrosphinganine protrudes from the enzyme, overview
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant Myc-His-tagged enzyme, Ni-NTA agarose
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Triton X-100, ammonium sulfate, calcium apatite, density gradient
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli, Myc-His-tagged enzyme
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expression in yeast cells
FVT1 expressed in CHO cells or yeast
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gene 3kdsr, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative expression analysis
S4SCP5
gene ksr1, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain JM109(DE3)
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gene ksrA, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain JM109(DE3)
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gene tsc10, expression of mutant tscDELTA38 in Escherichia coli strain BL21(DE3)
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genes At3g06060/TSC10A and At5g19200/TSC10B, i.e. tsc10A and tsc10B, expression of GFP-TSC10A and GFP-TSC10B in tsc10DELTA yeast mutant cells, heterologous expression of the two genes from Arabidopsis thaliana in 3-KDS reductase-deficient Saccharomyces cerevisiae mutant strain, tsc10DELTA, restores the enzyme activity
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TSC10p expressed in CHO cells or yeast
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the enzyme is upregulated showing increased expression during the bacterial challenge
S4SCP5
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
food industry
naturally occuring missense mutation A175T is linked to animals with bovine spinal muscular atrophy. Protein exhibits no detectable in vitro catalytic activity, but the mutated gene complements the growth defect of a homologous yeast knock-out strain as well as the healthy variant