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1.1.3.8: L-gulonolactone oxidase

This is an abbreviated version!
For detailed information about L-gulonolactone oxidase, go to the full flat file.

Word Map on EC 1.1.3.8

Reaction

L-gulono-1,4-lactone
+
O2
=
L-xylo-hex-2-ulono-1,4-lactone
 +
H2O2

Synonyms

At5g11540, AtGulLO2, AtGulLO3, AtGulLO5, GLO, GLOase, GulLO, GulLO3, GulLO5, GULO, gulonolactone oxidase, L-GulL oxidase, L-gulono-1,4-lactone dehydrogenase, L-gulono-1,4-lactone oxidase, L-gulono-gamma-lactone dehydrogenase, L-gulono-gamma-lactone oxidase, L-gulono-gamma-lactone: O2 oxidoreductase, L-gulono-gamma-lactone:oxidoreductase, L-gulonolactone oxidase, LGO

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.8 L-gulonolactone oxidase

Cloned

Cloned on EC 1.1.3.8 - L-gulonolactone oxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
biologically functional GLO activity is obtained by direct delivery of an expression vector containing the GLO cDNA into kidney of Silurus asotus, which lacks endogenous GLO
-
DNA and amino acid sequence determination and analysis, phylogenetic tree
-
expressed in COS1-cells
-
expressed in Escherichia coli JM109, transfected into guinea pig cell line 104C1
-
expressed in Nicotiana benthamiana leaves
expressed in silkworm cell line BmN4 with baculovirus vector
-
expressen in endothelial cell
-
expression in Nicothiana benthamiana
gene AtGulLO2, transgenic expression of the isozyme in Nicotiana tabacum BY-2 cells, the overexpression does not affect the cells. Expression of His-tagged isozyme in Escherichia coli strain BL21(DE3)pLysS is not successful
gene AtGulLO3, transgenic expression of the isozyme in Nicotiana tabacum BY-2 cells, the overexpression does not affect the cells. Expression of His-tagged isozyme in Escherichia coli strain BL21(DE3)pLysS is not successful
gene AtGulLO5, transgenic expression of the isozyme in Nicotiana tabacum BY-2 cells, the overexpression does not affect the cells. Expression of His-tagged isozyme in Escherichia coli strain BL21(DE3)pLysS is not successful
gene gulo, cloned from kidney, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time PCR expression analysis
overexpression in transgenic tomato fruits using the Agrobacterium tumefaciens strain EHA 105 and CaMV35S constitutive promoter in transfection, semi-quantitative RT-PCR expression analysis. Compared with wild-type, ascorbic acid is 1.5fold higher in red fruits from transgenic plants. Overexpression of GLOase improves tolerance to salt stress of tomato plant
-
quantitative real-time PCR expression analysis
-