1.1.3.6: cholesterol oxidase
This is an abbreviated version!
For detailed information about cholesterol oxidase, go to the full flat file.
Word Map on EC 1.1.3.6
-
1.1.3.6
-
biosensors
-
electrode
-
esterase
-
electrochemical
-
brevibacterium
-
fabric
-
oxidases
-
amperometric
-
film
-
rhodococcus
-
raft
-
cholestenone
-
voltammetry
-
cholest-4-en-3-one
-
flavoenzyme
-
cholesteryl
-
nanocomposite
-
biosensing
-
erythropolis
-
filipin
-
4-cholesten-3-one
-
nocardia
-
medicine
-
3hcholesterol
-
electropolymerization
-
lavendulae
-
biotechnology
-
agriculture
-
co-immobilized
-
methyl-beta-cyclodextrin
-
diagnostics
-
bioelectrode
-
luminol
-
screen-printed
-
electrocatalytic
-
analysis
-
3.1.1.13
-
polypyrrole
-
multiwalled
-
beta-ol
-
synthesis
-
drug development
- 1.1.3.6
-
biosensors
-
electrode
- esterase
-
electrochemical
- brevibacterium
-
fabric
- oxidases
-
amperometric
-
film
- rhodococcus
-
raft
- cholestenone
-
voltammetry
- cholest-4-en-3-one
-
flavoenzyme
-
cholesteryl
-
nanocomposite
-
biosensing
- erythropolis
- filipin
- 4-cholesten-3-one
- nocardia
- medicine
-
3hcholesterol
-
electropolymerization
- lavendulae
- biotechnology
- agriculture
-
co-immobilized
- methyl-beta-cyclodextrin
- diagnostics
-
bioelectrode
- luminol
-
screen-printed
-
electrocatalytic
- analysis
-
3.1.1.13
-
polypyrrole
-
multiwalled
- beta-ol
- synthesis
- drug development
Reaction
Synonyms
3beta-hydroxy steroid oxidoreductase, 3beta-hydroxysteroid: oxygen oxidoreductase, 3beta-hydroxysteroid:oxygen oxidoreductase, 3beta-hydroxysterol oxidase, BsChOx, CgChoA, CHO, CHO-U, ChO3, ChoA, choBb, CHOD, ChoG, ChoL, cholesterol oxidase, cholesterol oxidase I, cholesterol oxidase II, cholesterol-O2 oxidoreductase, CHOLOX, choM, ChoM1, ChoM2, choP, ChoRI, ChoRII, ChoS, ChOx, CO, CO1, COase, COD, COD-B, COX, HCEO-forming enzyme, HMPREF0204_11499, oxidase, cholesterol, PimE, ShChOx, type I ChOx
ECTree
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Substrates Products
Substrates Products on EC 1.1.3.6 - cholesterol oxidase
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REACTION DIAGRAM
1,4-androstadiene-3,17-dione + H2O
?
-
11% of the activity with cholesterol, enzyme CO1
-
-
?
17beta-hydroxymethyl-5-androsten-3beta-ol + O2
17beta-hydroxymethyl-5-androsten-3-one + H2O2
Nocardia erythropolis
-
-
-
?
20,22-dihydroxycholesterol + O2
20,22-dihydroxycholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
20,25-diazacholesterol + O2
20,25-diazacholest-4-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
20-hydroxycholesterol + O2
20-hydroxycholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
22-hydroxycholesterol + O2
22-hydroxycholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
23,24-dinor-5-cholen-3beta,22-diol + O2
23,24-dinor-5-cholen-22-ol-3-one + H2O2
Nocardia erythropolis
-
-
-
?
23,24-dinor-5-cholene-3beta,22-diol + O2
23,24-dinor-5-cholen-22-ol-3-one + H2O2
Nocardia erythropolis
-
-
-
-
r
24-hydroxycholesterol + O2
24-hydroxycholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
25-hydroxycholesterol + O2
25-hydroxycholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
4,4-dimethylcholesterol + O2
4,4-dimethylcholest-5-en-3-one
Nocardia erythropolis
-
-
-
?
4alpha-methylcholesterol + O2
4alpha-methylcholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
4beta-methylcholesterol + O2
4beta-methylcholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
5,7-cholestadien-3beta-ol + O2
5,7-cholestadien-3-one + H2O2
Nocardia erythropolis
-
-
-
?
5-androstene-3beta,17beta-diol + O2
5-androsten-17beta-ol-3-one + H2O2
Nocardia erythropolis
-
-
-
?
5-cholene-3beta,24-diol + O2
5-cholen-24-ol-3-one + H2O2
Nocardia erythropolis
-
-
-
?
5-pregnen-3beta,20beta-diol + O2
5-pregnen-20beta-ol-3-one + H2O2
Nocardia erythropolis
-
-
-
?
5alpha-androstan-3-ol-17-one + O2
5alpha-androstan-3,17-dione + H2O2
-
15% of the activity with cholesterol
-
-
?
5alpha-cholest-8(14)-en-3beta-ol + O2
5alpha-cholest-8(14)-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
beta-stigmasterol + O2
?
-
36% of the activity with cholesterol
-
-
?
beta-stigmasterol + O2
? + H2O2
-
68% activity compared to cholesterol
-
-
?
cholest-5-en-3beta-ol 7-one + O2
cholest-5-en-3,17-dione + H2O2
-
low activity with wild-type and mutant enzymes L119A, L119F, V145Q, P357N, Q286R and S379T
-
-
?
cholestenol + O2
5alpha-cholest-8-en-3-one + H2O2
-
43% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
?
-
27% of the activity with cholesterol
-
-
?
dehydroepiandrosterone + O2
? + H2O2
-
30% activity compared to cholesterol
-
-
?
epiandrosterone + O2
? + H2O2
-
15% activity compared to cholesterol
-
-
?
ergosta-5,7,22-trien-3beta-ol + O2
ergostan-5,7,22-trien-3-one + H2O2
-
18% of the activity with cholesterol
-
-
?
estrone + O2
estra-1,5(10)-dien-3,17-dione + H2O2
-
low activity with wild-type enzyme, no activity with mutant enzymes L119A, L119F, V145Q, P357N, Q286R and S379T
-
-
?
pregnenolone + O2
pregn-5-ene-3,20-dione + H2O2
low activity
-
-
?
sitost-5-en-3beta-ol + O2
sitost-5-en-3-one + H2O2
-
88% of the activity with cholesterol
-
-
?
stigmast-5-en-3beta-ol + O2
stigmast-5-en-3-one + H2O2
-
68% of the activity with cholesterol
-
-
?
26-hydroxycholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
?
26-hydroxycholesterol + O2
26-hydroxycholest-5-en-3-one + H2O2
Nocardia erythropolis
-
-
-
-
?
3beta-chlorocholest-5-ene + O2
? + H2O2
low activity
-
-
?
androst-5-en-3,17-dione + H2O2
-
30% of the activity with cholesterol
-
-
?
3beta-hydroxyandrost-5-en-17-one + O2
androst-5-en-3,17-dione + H2O2
-
30% of the activity with cholesterol
-
-
?
pregn-5-en-3,20-dione + H2O2
-
42% of the activity with cholesterol
-
-
?
3beta-hydroxypregn-5-en-20-one + O2
pregn-5-en-3,20-dione + H2O2
-
42% of the activity with cholesterol
-
-
?
5alpha-cholestan-3-one + H2O2
-
-
-
?
5alpha-cholestan-3beta-ol + O2
5alpha-cholestan-3-one + H2O2
-
13.2% of activity with cholesterol
-
-
?
5alpha-cholestan-3beta-ol + O2
5alpha-cholestan-3-one + H2O2
Nocardia erythropolis
-
-
-
?
5alpha-cholestan-3beta-ol + O2
5alpha-cholestan-3-one + H2O2
Nocardia erythropolis
-
87% of activity
-
?
5alpha-cholestan-3beta-ol + O2
5alpha-cholestan-3-one + H2O2
-
equally active as cholesterol
-
-
?
5alpha-cholestan-3beta-ol + O2
5alpha-cholestan-3-one + H2O2
-
equally active as cholesterol
-
-
?
5alpha-cholestan-3beta-ol + O2
5alpha-cholestan-3-one + H2O2
-
-
-
?
?
-
72% of the activity with cholesterol, enzyme CO1
-
-
?
5alpha-cholestane + O2
?
-
90% of the activity with cholesterol, enzyme CO2
-
-
?
5alpha-cholestane-3,7,dione
-
74% of the activity with cholesterol, enzyme CO1
-
-
?
5alpha-cholestane-3beta-ol-7-one + O2
5alpha-cholestane-3,7,dione
-
84% of the activity with cholesterol, enzyme CO2
-
-
?
cholest-4,7-dien-3-one + H2O2
-
14% of the activity with cholesterol
-
-
?
7-dehydrocholesterol + O2
cholest-4,7-dien-3-one + H2O2
-
26% of the activity with cholesterol
-
-
?
7alpha-hydroxy-cholest-4-en-3-one + H2O2
-
-
-
?
7alpha-hydroxy-cholesterol + O2
7alpha-hydroxy-cholest-4-en-3-one + H2O2
-
-
-
-
?
7alpha-hydroxy-cholesterol + O2
7alpha-hydroxy-cholest-4-en-3-one + H2O2
-
-
-
-
?
7beta-hydroxy-cholest-4-en-3-one + H2O2
-
-
-
?
7beta-hydroxy-cholesterol + O2
7beta-hydroxy-cholest-4-en-3-one + H2O2
-
-
-
-
?
7beta-hydroxy-cholesterol + O2
7beta-hydroxy-cholest-4-en-3-one + H2O2
-
-
-
-
?
7E,22-ergostadien-3-one + H2O2
-
-
-
?
7E,22-ergostadien-3beta-ol + O2
7E,22-ergostadien-3-one + H2O2
Nocardia erythropolis
-
-
-
?
7E,22-ergostadien-3beta-ol + O2
7E,22-ergostadien-3-one + H2O2
-
-
-
?
?
13% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
69% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
95% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
-
95% of the activity with cholesterol
-
-
?
beta-cholestanol + O2
?
75% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
-
101% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
Streptoverticillium cholesterolieum
-
96% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
-
79% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
?
-
79% activity compared to cholesterol
-
-
?
? + H2O2
-
96% activity compared to cholesterol
-
-
?
beta-cholestanol + O2
? + H2O2
Streptomyces rimosus MTCC 10792
-
96% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
?
-
about 55% of the activity with cholesterol, cholesterol oxidase II
-
-
?
beta-sitosterol + O2
? + H2O2
-
88% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
? + H2O2
Streptomyces rimosus MTCC 10792
-
88% activity compared to cholesterol
-
-
?
stigmast-5-en-3-one + H2O2
-
57% of the activity with cholesterol, enzyme CO1
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
79% of the activity with cholesterol, enzyme CO2
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
57% of the activity with cholesterol, enzyme CO1
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
79% of the activity with cholesterol, enzyme CO2
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
19.7% of activity with cholesterol
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
about 30% of the activity with cholesterol, cholesterol oxidase I
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
20% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
84% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
84% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
80% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
Corynebacterium cholesterolicum
-
50% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
19% of the activity with cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
29% of the activity with cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
64% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
85% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
85% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
64% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
72% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
64% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
Streptoverticillium cholesterolieum
-
39% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
29% activity compared to cholesterol
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
-
29% activity compared to cholesterol
-
-
?
stigmasta-5,22-dien-3-one + H2O2
10% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
59% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
36% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
Corynebacterium cholesterolicum
-
12% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
47% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
40% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
40% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
33% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
83% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
83% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
33% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
37% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
50% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
Streptoverticillium cholesterolieum
-
17% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
32% activity compared to cholesterol
-
-
?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
32% activity compared to cholesterol
-
-
?
campesterol + O2
(24R)-ergost-5-en-3-one + H2O2
Nocardia erythropolis
-
67% of activity with cholesterol
-
?
6beta-hydroxycholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
6beta-hydroxycholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
overview: substrate specificity
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
specific for steroid substrates with an OH group in position 3, preference for steroids with at least a C2 chain at position C17
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Corynebacterium cholesterolicum
-
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia erythropolis
-
-
the assumed intermediate 5-cholesten-3-one is rapidly isomerized by the enzyme to cholest-4-en-3-one
r
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia erythropolis
-
overview: substrate specificity
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia erythropolis
-
overview: substrate specificity
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia erythropolis
-
relatively unreactive 17keto-DELTA5-3beta-hydroxysteroids are converted to satisfactory substrates by formation of isopentyloximes or benzyloximes
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia erythropolis
-
size and shape of steroid 17beta-side-chain and oxygenation of nucleus
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Nocardia erythropolis
-
enzyme specifically oxidizes 3beta-hydroxy groups in steroids
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
enzyme specifically oxidizes 3beta-hydroxy groups in steroids
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
highest activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
specific for steroid substrates with an OH group in position 3, preference for steroids with at least a C2 chain at position C17
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
cholesterol oxidase is required for the biosynthesis of the antifungal polyene pimaricin
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
cholesterol oxidase is required for the biosynthesis of the antifungal polyene pimaricin, apparently acting as an antifungal sensor
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
via cholest-5-en-3-one
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
via cholest-5-en-3-one
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
overview: substrate specificity
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
Streptoverticillium cholesterolieum
-
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
100% activity
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
-
100% activity
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
first step in cholesterol degradation
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia erythropolis
-
first step in cholesterol degradation
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
Nocardia rhodochrous
-
first step in cholesterol degradation
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
-
subsequent isomerization to cholest-4-en-3-one
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
assay with phenazine sulfate and dichlorophenylindophenol as electron acceptors
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
a combination of molecular docking and molecular dynamics simulations is used to predict the binding interactions of CHL in the active site of ChOx. The hydrophobic triad residues (Y107A, F444 and Y446A) are observed to change conformation upon CHL binding in the wild-type form of the enzyme, leading to an optimal binding mode for catalytic efficiency
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
the enzyme catalyzes the oxidation of the substrate via a hydride transfer mechanism and concomitant reduction of the FAD cofactor. The Glu361 residue acts as a catalytic base facilitating the hydride transfer from the substrate to the cofactor
-
-
?
cholesterol + O2
cholest-5-en-3-one + H2O2
-
first step in cholesterol degradation
-
?
?
-
10% of the activity with cholesterol, enzyme CO2
-
-
?
cholesterol-5alpha,6beta-epoxide + O2
?
-
9% of the activity with cholesterol, enzyme CO1
-
-
?
?
-
63% of the activity with cholesterol, enzyme CO1
-
-
?
coprostane + O2
?
-
67% of the activity with cholesterol, enzyme CO2
-
-
?
?
-
about 35% of the activity with cholesterol, cholesterol oxidase I
-
-
?
dehydro-epi-androsterone + O2
?
-
about 5% of the activity with cholesterol, cholesterol oxidase II
-
-
?
androst-5-en-3,17-dione + H2O2
-
41.4% of the activity with cholesterol
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
41% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
16% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
16% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
27% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
Corynebacterium cholesterolicum
-
5% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
-
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
-
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
-
28% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
24% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
-
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
-
37% activity compared to cholesterol
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
-
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
-
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
Streptoverticillium cholesterolieum
-
48% activity compared to cholesterol
-
-
?
dehydroisoandrosterone + O2
androst-5-en-3,17-dione + H2O
-
low activity with wild-type and mutant enzymes L119A, L119F, V145Q, P357N, Q286R and S379T
-
-
?
desmosterol + O2
cholesta-5,24-dien-3-one + H2O2
Nocardia erythropolis
-
98% of activity with cholesteol
-
?
5alpha-cholestan-3-one + H2O2
-
58% of the activity with cholesterol, enzyme CO1
-
-
?
dihydrocholesterol + O2
5alpha-cholestan-3-one + H2O2
-
70% of the activity with cholesterol, enzyme CO2
-
-
?
dihydrocholesterol + O2
5alpha-cholestan-3-one + H2O2
-
22% of the activity with cholesterol
-
-
?
dihydrocholesterol + O2
5alpha-cholestan-3-one + H2O2
-
79% of the activity with cholesterol
-
-
?
diosgenone + H2O2
i.e. (25R)-spirost-5-en-3beta-ol
-
-
?
diosgenin + O2
diosgenone + H2O2
i.e. (25R)-spirost-5-en-3beta-ol
-
-
?
?
10% activity compared to cholesterol
-
-
?
ergosterol + O2
? + H2O2
Streptomyces rimosus MTCC 10792
-
12% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
20% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
20% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
12% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
Corynebacterium cholesterolicum
-
4% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
-
17% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
-
51% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
-
51% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
12% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
-
14% activity compared to cholesterol
-
-
?
ergosterol + O2
dehydroergosterol + H2O2
Streptoverticillium cholesterolieum
-
5% activity compared to cholesterol
-
-
?
5alpha-(25R)-spirostan-3,12-dione + H2O2
-
66% of the activity with cholesterol, enzyme CO1
-
-
?
hecogenin + O2
5alpha-(25R)-spirostan-3,12-dione + H2O2
-
80% of the activity with cholesterol, enzyme CO2
-
-
?
5alpha-lanosta-8,24-dien-3-one + H2O2
-
4% of the activity with cholesterol, enzyme CO1
-
-
?
lanosterol + O2
5alpha-lanosta-8,24-dien-3-one + H2O2
-
5% of the activity with cholesterol, enzyme CO2
-
-
?
5-pregnen-3,20-dione + H2O2
-
22.4% of activity with chelesterol
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
22% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
32% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
32% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
95% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
Corynebacterium cholesterolicum
-
38% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
-
6% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
-
20% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
99% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
-
42% activity compared to cholesterol
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
Streptoverticillium cholesterolieum
-
37% activity compared to cholesterol
-
-
?
pregnenolone + O2
?
-
about 60% of the activity with cholesterol, cholesterol oxidase I
-
-
?
pregnenolone + O2
?
-
about 60% of the activity with cholesterol, cholesterol oxidase II
-
-
?
pregnenolone + O2
? + H2O2
Streptomyces rimosus MTCC 10792
-
46% activity compared to cholesterol
-
-
?
stigmastanone + H2O2
-
15% of the activity with cholesterol
-
-
?
stigmastanol + O2
stigmastanone + H2O2
-
7% of the activity with cholesterol
-
-
?
stigmasta-5,22-dien-3-one + H2O2
-
102% of the activity with cholesterol, enzyme CO2
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
86% of the activity with cholesterol, enzyme CO1
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
102% of the activity with cholesterol, enzyme CO2
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
86% of the activity with cholesterol, enzyme CO1
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
10% of activity with cholesterol
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
about 10% of the activity with cholesterol, cholesterol oxidase I
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
about 10% of the activity with cholesterol, cholesterol oxidase II
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
15% of the activity with cholesterol
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
-
32% of the activity with cholesterol
-
-
?
?
-
-
optimization of assay conditions, concerning buffer type and molarity, temperature, pH, deergent addition, ionogenic state etc.
-
-
?
additional information
?
-
no activity with ergosterol
-
-
?
additional information
?
-
cholesterol oxidase is a bifunctional enzyme that catalyzes the oxidation and isomerization of cholesterol to cholest-4-en-3-one while dioxygen is finally reduced to H2O2 as by-product. The enzymatic overall cholesterol oxidation comprises three steps. In the first one the 3beta-OH group of cholesterol is oxidized to the corresponding ketone with the concomitant reduction of the FAD cofactor. In a second step an isomerization of the double bond from the DELTA5-6 position to the DELTA4-5 position takes place. The FAD is recycled in a redox reaction with dioxygen, yielding hydrogen peroxide
-
-
?
additional information
?
-
-
cholesterol oxidase is a bifunctional enzyme that catalyzes the oxidation and isomerization of cholesterol to cholest-4-en-3-one while dioxygen is finally reduced to H2O2 as by-product. The enzymatic overall cholesterol oxidation comprises three steps. In the first one the 3beta-OH group of cholesterol is oxidized to the corresponding ketone with the concomitant reduction of the FAD cofactor. In a second step an isomerization of the double bond from the DELTA5-6 position to the DELTA4-5 position takes place. The FAD is recycled in a redox reaction with dioxygen, yielding hydrogen peroxide
-
-
?
additional information
?
-
the enzyme also acts as a DELTA5-ketosteroid isomerase, EC 5.3.3.1, converting a 3-oxo-DELTA5-steroid to a 3-oxo-DELTA4-steroid
-
-
?
additional information
?
-
-
the enzyme also acts as a DELTA5-ketosteroid isomerase, EC 5.3.3.1, converting a 3-oxo-DELTA5-steroid to a 3-oxo-DELTA4-steroid
-
-
?
additional information
?
-
cholesterol oxidase is a bifunctional enzyme that catalyzes the oxidation and isomerization of cholesterol to cholest-4-en-3-one while dioxygen is finally reduced to H2O2 as by-product. The enzymatic overall cholesterol oxidation comprises three steps. In the first one the 3beta-OH group of cholesterol is oxidized to the corresponding ketone with the concomitant reduction of the FAD cofactor. In a second step an isomerization of the double bond from the DELTA5-6 position to the DELTA4-5 position takes place. The FAD is recycled in a redox reaction with dioxygen, yielding hydrogen peroxide
-
-
?
additional information
?
-
the enzyme also acts as a DELTA5-ketosteroid isomerase, EC 5.3.3.1, converting a 3-oxo-DELTA5-steroid to a 3-oxo-DELTA4-steroid
-
-
?
additional information
?
-
-
less than 3% of the activity with cholesterol: ergosterol and epiandrosterone. No activity: dehydroepiandrosterone, pregnenolone
-
-
?
additional information
?
-
-
no activity with: ergosterol, dehydroepiandrosterone, epiandrosterone, pregnenolone
-
-
?
additional information
?
-
-
intact ChoD is important for the growth of Mycobacterium tuberculosis in peritoneal macrophages and lungs of mice. Cholesterol oxidase is required for virulence of Mycobacterium tuberculosis
-
-
?
additional information
?
-
the bifunctional FAD-dependent enzyme catalyzes the oxidation and isomerization of sterols to sterones, typically cholesterol (5-cholesten-3-beta-ol) to 4-cholesten-3-one (cholestenone) and H2O2. The enzyme shows higher activity towards the 3beta-hydroxy steroids with long alkyl chains at C17
-
-
?
additional information
?
-
the bifunctional FAD-dependent enzyme catalyzes the oxidation and isomerization of sterols to sterones, typically cholesterol (5-cholesten-3-beta-ol) to 4-cholesten-3-one (cholestenone) and H2O2. The enzyme shows higher activity towards the 3beta-hydroxy steroids with long alkyl chains at C17
-
-
?
additional information
?
-
the bifunctional FAD-dependent enzyme catalyzes the oxidation and isomerization of sterols to sterones, typically cholesterol (5-cholesten-3-beta-ol) to 4-cholesten-3-one (cholestenone) and H2O2. The enzyme shows higher activity towards the 3beta-hydroxy steroids with long alkyl chains at C17
-
-
?
additional information
?
-
the bifunctional FAD-dependent enzyme catalyzes the oxidation and isomerization of sterols to sterones, typically cholesterol (5-cholesten-3-beta-ol) to 4-cholesten-3-one (cholestenone) and H2O2. The enzyme shows higher activity towards the 3beta-hydroxy steroids with long alkyl chains at C17
-
-
?
additional information
?
-
the enzyme oxidizes 3beta-hydroxysteroids such as cholesterol, beta-cholestanol, and beta-sitosterol at high rates. No activity with 5alpha-androstan-3beta-ol-17-one, and 3beta-hydroxyandrost-5-en-17-one
-
-
?
additional information
?
-
-
the enzyme oxidizes 3beta-hydroxysteroids such as cholesterol, beta-cholestanol, and beta-sitosterol at high rates. No activity with 5alpha-androstan-3beta-ol-17-one, and 3beta-hydroxyandrost-5-en-17-one
-
-
?
additional information
?
-
the enzyme oxidizes 3beta-hydroxysteroids such as cholesterol, beta-cholestanol, and beta-sitosterol at high rates. No activity with 5alpha-androstan-3beta-ol-17-one, and 3beta-hydroxyandrost-5-en-17-one
-
-
?
additional information
?
-
-
no activity with dehydroepiandrosterone
-
-
?
additional information
?
-
the oxidation of the allylic alcohols by cholesterol oxidase from Rhodococcus erythropolis is stereo- and enantioselective. The enzyme oxidizes (S)-alcohol at a higher rate than (R)-alcohol
-
-
?
additional information
?
-
no activity with cholic acid, progesterone and 4-androsten-3,17-dione
-
-
?
additional information
?
-
the enzyme does not use glycerol as substrate
-
-
?
additional information
?
-
-
the enzyme does not use glycerol as substrate
-
-
?
additional information
?
-
-
the enzyme shows substrate specificity for 3beta-hydroxysteroids. No activity with cholic acid and cholesteryl linoleate
-
-
?
additional information
?
-
the enzyme does not use glycerol as substrate
-
-
?
additional information
?
-
-
the enzyme shows substrate specificity for 3beta-hydroxysteroids. No activity with cholic acid and cholesteryl linoleate
-
-
?
additional information
?
-
no activity with cholic acid, progesterone and 4-androsten-3,17-dione
-
-
?
additional information
?
-
-
cholesterol oxidase is involved in pimaricin biosynthesis
-
-
?
additional information
?
-
-
no activity with cholest-5-en-3alpha-ol
-
-
?
additional information
?
-
-
no activity with cholest-5-en-3alpha-ol
-
-
?
additional information
?
-
-
the wild-type enzyme also shows cholesterol dehydrogenase activity with 0.001 U/mg
-
-
?
additional information
?
-
-
the enzyme catalyzes the oxidation of steroid substrates which have a hydroxyl group at the 3beta-position of the steroid ring system
-
-
?
additional information
?
-
-
cholesterol oxidase is a bifunctional FAD-containing oxidoreductase which catalyzes the oxidation and isomerization of cholesterol into 4-cholesten-3-one
-
-
?
additional information
?
-
cholesterol oxidase is a bifunctional flavoenzyme that catalyzes the oxidation and isomerization of cholesterol to cholest-4-en-3-one
-
-
?
additional information
?
-
the enzyme catalyzes the oxidation and isomerization of steroids containing a 3-hydroxyl group, with the cholestane group conferring binding specificity in the active site. The enzyme is also able to oxidize small-molecule alcohols such as 2-propanol and methanol, with a preference for those containing aromatic ring, but with lower activity. Substrate oxidation results in reduction of the cofactor during the reductive half reaction. An oxidative half reaction occurs with re-oxidation of the flavin cofactor by molecular oxygen to form hydrogen peroxide. Structure-function relationship and analysis overview
-
-
?
additional information
?
-
-
no activity with ergosterol, pregnenolone, dehydroepiandrosterone, and epiandosterone
-
-
?
additional information
?
-
-
no activity with ergosterol, pregnenolone, dehydroepiandrosterone, and epiandosterone
-
-
?