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1.1.3.17: choline oxidase

This is an abbreviated version!
For detailed information about choline oxidase, go to the full flat file.

Word Map on EC 1.1.3.17

Reaction

Betaine aldehyde
+
O2
 +
H2O
=
betaine
 +
H2O2

Synonyms

alkaliphilic choline oxidase, ANI01nite_22550, An_CodA, APChO-syn, CHO, choline oxidase, choline-oxygen 1-oxidoreductase, choline:oxygen 1-reductase, ChOx, ChOx protein, codA, COX

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.17 choline oxidase

Renatured

Renatured on EC 1.1.3.17 - choline oxidase

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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
conformational change reverting the enzyme to the native form, rate of approaching steady state independent of concentrations of choline and enzyme, increased to a limiting value with increasing pH
fully oxidized form of the enzyme is obtained via slow oxidation of the flavosemiquinone by extensive dialysis at pH 6 and 4°C
incubation of the inactive enzyme at pH values of pH 6.5 and above, and 25°C results in a fast and partial reactivation of the enzyme, which occurrs with slow onset of steady state during enzymatic turnover, overview
-
Systematic exchange of individual segments of the Arthrobacter pascens gene with segments from a choline oxidase-encoding gene from Arthrobacter globiformis yielding a functional chimeric enzyme when produced in Escherichia coli
-