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1.1.3.17: choline oxidase

This is an abbreviated version!
For detailed information about choline oxidase, go to the full flat file.

Word Map on EC 1.1.3.17

Reaction

Betaine aldehyde
+
O2
+
H2O
=
betaine
+
H2O2

Synonyms

alkaliphilic choline oxidase, ANI01nite_22550, An_CodA, APChO-syn, CHO, choline oxidase, choline-oxygen 1-oxidoreductase, choline:oxygen 1-reductase, ChOx, ChOx protein, codA, COX

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.17 choline oxidase

Cloned

Cloned on EC 1.1.3.17 - choline oxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Arabidopsis thaliana transformed with codA gene from Arthrobacter globiformis which encodes choline oxidase
-
Balb/c mice used for toxicity studies of choline oxidase - no significant difference from control in terms of growth, body weight, food consumption, and blood biochemical indices. Analysis of various tissues show no significant effect
-
choline oxidase from Arthrobacter pascens facilitates adaptation to osmotic stress in Escherichia coli
-
cloning of Sinorhizobium meliloti bet genes, Tn5 mutant LtS23-1020 is completed by plasmid pCHO34
-
codA gene introduced into Synecochoccus sp. PCC 7942 and Arabidopsis thaliana leads to accumulation of glycinebetaine and enhanced tolerance to salt and cold stress, rice Oryza sativa L. genetically engineered, Agrobacterium-mediated transformation, ability to synthesize glycinebetaine is established by introducing the codA gene for choline oxidase from Arthrobacter globiformis
-
expressed in Escherichia coli from plasmid pET/codA1
expressed in Escherichia coli Rosetta(DE3)pLysS cells
expressed in Escherichia coli, plant expression vector pGAH/codA,codA gene and nos terminator fragments excised from pGAH/codA, ligated to stress-inducible SWPA2 promoter, fused into pCAMBIA3300 binary vector, resulting pScodA transformed into Solanum tuberosum by using Agrobacterium tumefaciens strain AGL0
-
expressed in Escherichia coli, plasmid pET/codA1
expressed in Escherichia coli, strain Rosetta(DE3)pLysS, pET/codAmg plasmid, wild-type and Glu312 variants generated by site-directed mutagenesis
expressed in Escherichia coli, wild-type and H351A variant, plasmid pET/codAmg1-H351A
expressed in Eucalyptus globulus
expression of wild-type and mutant enzymes in Escherichia coli
-
expression of wild-type and mutant enzymes in Escherichia coli strain Rosetta(DE3)pLysS
-
expression of wild-type and mutants in Escherichia coli strain Rosetta(DE3)pLysS
gene codA, expression in Euccalyptus globulus via Agrobaccterium tumefaciens strain EHA105 transfection method using vectors pBI121 and pGW23codA
-
gene codA, expression of Strep-tagged wild-type and mutant enzymes in Escherichia coli strain BL21-Gold
-
heterologous expression in Escherichia coli DH5 alpha and BL21(DE3), respectively. Systematic exchange of individual segments of the Arthrobacter pascens gene with segments from a choline oxidase-encoding gene from Arthrobacter globiformis yielding a functional chimeric enzyme
-
mutant enzyme S101A is expressed in Escherichia coli Rosetta(DE3)pLysS cells
overexpression of the enzyme, fused to a chloroplast-transit peptide from Pisum sativum, in transgenic Oryza sativa plants using the Agrobacterium tumefaciens transfection system
-
recombinant expression in Escherichia coli
recombinant expression in Escherichia coli strain Rosetta (DE3)pLysS