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1.1.3.13: alcohol oxidase

This is an abbreviated version!
For detailed information about alcohol oxidase, go to the full flat file.

Word Map on EC 1.1.3.13

Reaction

a primary alcohol
+
O2
=
an aldehyde
+
H2O2

Synonyms

alcohol oxidase 1, alcohol oxidase 2, alcohol oxidase A, alcohol oxidase B, alcohol oxidase I, alcohol: O2 oxidoreductase, alcohol:dioxygen-oxidoreductase, alcohol:O2 oxidoreductase, AlcOx, AOd, AOD1, AOext, AOint, AOX, AOX1, AOX2, AOXI, broad substrate specific alcohol oxidase, EC 1.1.3.31, ethanol oxidase, extracellular alcohol oxidase, FAD-dependent alcohol oxidase, FAO1, GLRG_05590, intracellular alcohol oxidase, long chain fatty alcohol oxidase, methanol oxidase, Mod1p, Mod2p, oxidase, alcohol, P-AOD, peroxisomal alcohol oxidase, primary alcohol oxidase, SCAO, short chain alcohol oxidase, short-chain alcohol oxidase, VAO, veratryl alcohol oxidase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.13 alcohol oxidase

Purification

Purification on EC 1.1.3.13 - alcohol oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation
-
ammonium sulfate precipitation, DEAE-cellulose column chromatography, Sepharose 2B column chromatography, and Sephadex G-25 gel filtration
Pichia putida
-
ammonium sulfate precipitation, phenyl-Toyopearl 650 M resin column chromatography, Q-Toyopearl 650 M column chromatography, and hydroxyapatite column chromatography
-
CIM isobutyl column can be used as a hydrophobic column for the purification of P-AOD
-
DEAE-cellulose column chromatography and Biogel P-100 gel filtration
-
native enzyme 490fold in three steps by anion exchange and hydrophobic interaction chromatographies, using a convection interaction media isobutyl disc monolithic column, preparation, overview, to homogeneity
-
native enzyme by microsomal membrane preparation by differential centrifugation. The enzyme is solubilized from the microsomal membrane using 0.5% (v/v) 3-[(3-cholamidopropyl) dimethyl ammonio] propane-1-sulfonic acid, i.e. CHAPS, and further purified by ammonium sulfate fractionation followed by anion exchange chromatography
-
native enzyme from strain AIU 063 in a three-step chromatographic process
-
native enzyme to homogeneity
-
native enzyme to homogeneity by two different steps of anion exchange chromatography
-
native intracellular enzyme, 9.24fold, and extracellular enzyme, 314fol, from strain NBRC 31693, by streptomycin precipitation, dialysis, anion exchange chromatography and gel filtration or by polethylene glycol precipitation, anion exchange and hydrophobic interaction chromatography, and gel filtration, respectively
-
Q-Sepharose column chromatography and Superose 6 gel filtration
recombinant C-terminally His6-tagged enzyme from Pichia pastoris strain GS115 by nickel affinity chromatography and gel filtration. The recombinant enzyme shows high thermal stability and resistance to H2O2 inhibition
-
recombinant enzyme 7.7fold from overproducing Hansenula polymorpha strain C-105 by ammonium sulfate fractionation and ion exchange chromatography to homogeneity
-
single step chromatographic separation on a DEAE-Sepharose column
Superdex 75 gel filtration