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1,12-dichlorododecane
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-
2,2'-dipyridyl
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1 mM, 13% inhibition
2-Aminoethanol
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1 mM, 70% inhibition
3-Pentanol
-
1 mM, 16% inhibition
4-chloromercuribenzoate
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0.1 mM, 31% inhibition
5,5'-dithiobis(2-nitrobenzoate)
5,5'-dithiobis-(2-nitrobenzoic acid)
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-
8-hydroxyquinoline
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1 mM, 6% inhibition
AgNO3
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1 mM, complete loss of activity
carbonyl cyanide m-chlorophenyl hydrazone
competitive with respect to FAD
-
dinitrophenol
competitive with respect to FAD
dithiothreitol
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85% residual activity at 0.5 mM
DMSO
-
complete inhibition by 50% DMSO
Fe2+
-
slight inhibition of extracellular enzyme
HgCl2
-
1 mM, 44% inhibition
imidazole
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1 mM, 20% inhibition
iodoacetic acid
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59% residual activity at 1 mM
KBr
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3.5 M at 30°C for 1-2 h: partial inactivation, KBr + urea: complete inactivation
L-cysteine
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70% residual activity at 0.5 mM
Mercuric acetate
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0.1 mM, complete inhibition
Mercuric chloride
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1 mM, complete inhibition
metal chelators
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slight
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methanol
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substrate inhibition
Mo6+
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1 mM, 94% inhibition
Monoiodoacetic acid
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1 mM, 31% inhibition
N-ethylmaleimide
-
84% residual activity at 1 mM
Na+
-
slight inhibition of extracellular enzyme
o-phenanthroline
-
93% residual activity at 1 mM
p-chloromercuribenzoate
Pichia putida
-
complete inhibition at molar excess of reagents in relation to alcohol oxidase 20:1
p-chloromercuribenzoic acid
-
-
p-hydroxymercuribenzoate
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1 mM, complete inhibition
propargyl alcohol
-
irreversible
Semicarbazide
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91% residual activity at 1 mM
Tetraethylthiuram disulfide
-
5 mM, more than 70% loss of activity
Thiosemicarbazide
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1 mM, 31% loss of activity
Urea
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6 M, at 30°C for 1-2 h: partial inactivation, urea + KBr: complete inactivation
Zn2+
-
35% residual activity at 0.5 mM
1,10-phenanthroline
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1 mM, 56% loss of activity
1,10-phenanthroline
-
1 mM, 10% inhibition
1,4-butynediol
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irreversible
4-Hydroxy-2-butynal
-
-
4-Hydroxy-2-butynal
-
irreversible
5,5'-dithiobis(2-nitrobenzoate)
Pichia putida
-
complete inhibition at molar excess of reagents in relation to alcohol oxidase 20:1
5,5'-dithiobis(2-nitrobenzoate)
-
1 mM, 35% inhibition
acetamide
-
-
acetamide
-
1 mM, 10% inhibition
Ag+
-
-
Cd2+
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1 mM, 48% inhibition
Cd2+
-
65% residual activity at 0.5 mM
Cu2+
-
1 mM, 86% inhibition
Cu2+
-
15% residual activity at 0.5 mM
Cu2+
-
91% residual activity at 1 mM
Cu2+
-
1 mM CuCl2, complete inhibition
Cu2+
-
1 mM CuSO4, 50% inhibition
Cu2+
-
1 mM, 95% inhibition
Cu2+
-
reversible by EDTA
Cu2+
-
1 mM CuSO4, 30 min, strong
Cyclopropanone
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-
Cyclopropanone
-
suicide substrate
Cyclopropanone
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0.05 mM, pH 7.5, 20°C, pseudo-first order loss of enzymatic activity, t1/2: 39 min, no reversal by removal of the inhibitor, rate of inactivation is reduced in the presence of substrate, product, or glutathione, inactivation is completely prevented by reduction of the FAD coenzyme prior to addition of the inactivator
diethyldicarbonate
-
-
EDTA
-
75% residual activity at 0.5 mM
EDTA
-
91% residual activity at 1 mM
EDTA
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slight inhibition of intra- and extracellular enzymes
FeSO4
-
-
FeSO4
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1 mM, complete inhibition
formaldehyde
-
-
H2O2
-
-
H2O2
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inhibits at 5 mM, at 7.8 mM the enzyme maintains 50% of its initial activity after 0.5 h of incubation at 4°C
H2O2
-
methanol or catalase partially protects
Hg2+
-
0.1 mM, complete inhibition
Hg2+
-
52% residual activity at 0.5 mM
Hg2+
-
strong inhibition of intra- and extracellular enzymes
Hg2+
-
reversible by mercaptoethanol
Hg2+
-
1 mM HgCl2, 30 min, strong
hydrazine
-
1 mM, complete inhibition
hydrazine
-
89% residual activity at 1 mM
hydroquinone
-
-
hydroquinone
-
1 mM, 30 min, strong
hydroxylamine
-
1 mM, complete inhibition
hydroxylamine
-
72% residual activity at 1 mM
hydroxylamine
-
1 mM, 21% inhibition
iodoacetate
-
-
iodoacetate
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1 mM, 38% inhibition
iodoacetate
-
time-dependent inactivation, reversible on removal of excess inhibitor
K+
-
75% residual activity at 0.5 mM
K+
-
slight inhibition of extracellular enzyme
KCN
-
1 mM 56% inhibition
KCN
-
93% residual activity at 1 mM
KCN
-
inhibition of intra- and extracellular enzymes
Mg2+
-
90% residual activity at 0.5 mM
Mg2+
-
93% residual activity at 1 mM
Mn2+
-
62% residual activity at 0.5 mM
Mn2+
-
slight inhibition of intra- and extracellular enzymes
NaN3
-
-
NaN3
-
competitive, reversible
NaN3
Poria contigua
-
strong
NaN3
-
inhibition of intra- and extracellular enzymes
NEM
-
1 mM, 10% inhibition
NEM
-
slight inhibition of intra- and extracellular enzymes
NEM
-
1 mM, 30 min, strong
NiCl2
-
-
NiCl2
-
1 mM, complete inhibition
PCMB
-
5 mM, complete loss of activity
PCMB
-
0.1 mM; 39% inhibition
PCMB
Poria contigua
-
1 mM, complete inhibition
PCMB
-
1 mM, complete inhibition
PCMB
-
t1/2: 11 min, beta-mercaptoacetic acid restores activity, substrates or products slow the inactivation rate
PCMB
-
1 mM, 30 min, strong
phenylhydrazine
-
1 mM, complete inhibition
phenylhydrazine
-
1 mM, complete inhibition
phenylhydrazine
-
1% residual activity at 1 mM
Propynal
-
-
Sodium acetate
-
-
Sodium azide
-
the enzyme is completely inhibited by 0.5 mM sodium azide
Sodium azide
-
97% residual activity at 1 mM
Sodium azide
-
1 mM, 15% inhibition
additional information
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no inhibition by ethylene glycol, even at high concentrations
-
additional information
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not inhibited by Ba2+, Ca2+, and Na+
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additional information
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glycerol represses enzyme expression, the enzyme expression is derepressed under glycerol limitations, growth on both methanol and glycerol are not repressed
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additional information
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2-propin-1-ol and methylenecyclopropyl alcohol are not suicide substrates
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